Tuck-Weng Kok Curriculum Vitae January 2019

Home , SA Pathology, University of Adelaide

University of Adelaide School of Biological Sciences Department of Molecular & Cellular Biology North Terrace, Adelaide South Australia 5005. [email protected] Mobile +61- 410 691 268

EDUCATION & QUALIFICATIONS

FFSc (RCPA) Founding Fellow, Faculty of Science, Royal College of Pathologists - Australasia 2010 FASM Fellow of the Australian Society for Microbiology 2007 Dissertation: HIV Unintegrated DNA is Transcriptionally Active PhD University of Adelaide 1998 Thesis: Early events in the replication cycle of human immunodeficiency virus MSc University of Adelaide 1986 Thesis: Improved Laboratory Diagnosis of Mycoplasma pneumoniae infection BSc (Hons) University of Adelaide 1976 (Immunology)

Adjunct Academic Affiliations

2017 - Visiting Research Fellow, Sch of Biological Sciences, University of Adelaide 2013 - 2017 Professor - School of Medical Sciences, RMIT University, Melbourne. 2010 - Assoc. Professor – Adelaide Medical School, University of Adelaide 2009 - 2014 Assoc. Professor - School of Pharmacy & Medical Sciences, Uni. South Australia

PROFESSIONAL APPOINTMENTS

2017 - Principal Examiner (Microbiology) Royal College Pathologists Australasia 2014 - Editor, Journal of Virological Methods (Elsevier) 2009-14 Chief Medical Scientist (Excellence level), IMVS - SA Pathology 2003-04 World Health Organization (Virology Consultant) Beijing, P. R. China 2000-09 Chief Medical Scientist (Virology), IMVS - SA Pathology 1998-99 Consultant Microbiologist, Cooperative Research Centre for Molecular Engineering and Technology, Sydney 1996-98 Principal Medical Scientist, Head of Virology Laboratory, Institute of Medical & Veterinary Science (IMVS), Adelaide. 1981-95 Senior Medical Scientist, Head of Rapid Viral Diagnostic and Cell Culture Laboratories, IMVS. 1976-80 Medical scientist, Division of Clinical Microbiology, IMVS. 1975-76 Research Assistant, Dept. Human Physiology & Pharmacology, University of Adelaide.

PUBLICATIONS (peer-reviewed)

2019 Sequencing and analysis of globally obtained human parainfluenza viruses 1 and 3 genomes.

M. E. Bose et al. (2019) PLoS One (submitted)

2018 Mycoplasma pneumoniae DNA detection and specific antibody class response in patients from two tertiary care hospitals in tropical Sri Lanka. Lakmini Wijesooriya, Tuckweng Kok, Jennifer Perera, Y. Tilakarathne and N.P. Suni- Chandra. Journal of Medical Microbiology (2018) 67:1232 - 42, doi: 10.1099/jmm.0.000813

2018 Colocalization of intracellular specific IgA (icIgA) with influenza virus in patients' nasopharyngeal aspirate cells TuckWeng Kok, Mauritzio Costabile, Greg Tannock and Peng Li J. Virological Methods (2018), 252: 8-14.

2016 A Histopathological Re-examination of a Fatal Ten-Year Multisystem Dysfunctional Illness after Acute Encephalitis Coxiella Dormancy and Pathogenesis in Slow Motion Olga Sukocheva, Jim Manavis, Tuck-Weng Kok*, Mark Turra, Angelo Izzo, Peter Blumbergs and Barrie Marmion. BMC Infectious Diseases 2016, 16:165 doi:10.1186/s12879-016-1497-z

2014 Michael E Bose, Jie He, Susmita Shrivastava, Martha I Nelson,..Tuckweng Kok,..and Kelly Henrickson. Sequencing and Analysis of Global Human Respiratory Syncytial Virus A and B Genome Sequences. PLoS ONE March 20, 2015 DOI: 10.1371/journal.pone.0120098

2014 Tuckweng Kok, A. J. Gaeguta, J. Finnie, P R Gorry, M. Churchill & Peng Li (2014) Designer Antigens for Elicitation of Broadly Neutralizing Antibodies Against HIV Clinical & Translational Immunology Sep 2014; 3(9): e24.

2013 Leang, S. K...Kok, T..and Hurt, A. C. Influenza antiviral resistance in the Asia-Pacific region during 2011. Antiviral Research 97: 206-10

2013 The volume of viral inoculums used is critical in experimental influenza A infection in mice. Darren Miller, Tuckweng Kok and Peng Li. Laboratory Animals 47(1): 74-7.

2011 Surface scattering Plasmon resonance fibre sensors: demonstration of rapid Influenza A virus detection. A. Francois, J. Boehm, S.Y. Oh, T. Kok and T. Monro. Proceedings of SPIE Defense, Security and Sensing

2010 Francois, A., Boehm, J., Oh, S. Y., Kok, T. & Monro, T. M. Collection mode surface plasmon fibre sensors: a new biosensing platform. Biosensors & bioelectronics 26, 3154-59.

2011 Darren Miller, John Finnie, Timothy Bowden, Anita Scholz, Sawyin Oh, Tuckweng Kok*, Christopher Burrell, Lee Trinidad, David Boyle, Peng Li. Preclinical Efficacy Studies of Influenza A Haemagglutinin Precursor Cleavage Loop Peptides as a Potential Vaccine. J. General Virology 92: 1152-61

2010 Kok, T. & Gosbell, I. B. Editorial First Words: Diagnostic Microbiology in the 21st Century - A Quiet Revolution. Microbiology Australia 31, 102-103.

2010 Roberts, J. A., Kok, T. & Thorley, B. New Approaches to Enterovirus Identification. Microbiology Australia 31, 138-141.

2009 Q fever: persistence of antigenic non-viable cell residues of Coxiella burnetii in the host-- implications for post Q fever infection fatigue syndrome and other chronic sequelae BP Marmion, O. Sukocheva, PA Storm, M. Lockhart, M. Turra, T. Kok, ... and S. Graves. Quarterly J. of Medicine 102 (10): 673-84.

2009 Emergence and spread of oseltamivir-resistant A (H1N1) influenza viruses in Oceania, South East Asia and South Africa. A. Hurt, J. Ernest,..T. Kok . I. Barr. Antiviral Research 83: 90- 3.

2009 Neutralizing monoclonal antibodies to different clades of Influenza A H5N1 viruses SY Oh, P Selleck, P Chan, P. Lim, G. Higgins, C J Burrell, TW Kok J Virological Methods 157:161-7

2008 Rapid Detection of Influenza A Virus in Clinical Samples Using an Ion Channel Switch Biosensor (2008). SY Oh, B A Cornell, D Smith, G. Higgins, C J Burrell, TW Kok Biosensors and Bioelectronics 23: 1161-5

2007 Ratcliff, R. M., G. Chang, T. Kok and T. P. Sloots. Molecular diagnosis of medical viruses Current Issues Molecular Biology 9: 87-102.

2007 Real-time PCR for Detection of Trichomonas vaginalis in Female Genital Specimens Paul Simpson, Geoff Higgins, Qiao Ming and Tuckweng Kok. Journal of Medical Microbiology 56: 772-777

2006 Human Immunodeficiency Viral Unintegrated DNA is Transcriptionally Active TuckWeng Kok. Thesis by peer-reviewed examination for Fellow of the Australian Society for Microbiology. Pg. 1-21.

2004 Tuckweng Kok. Review, Assessment and Recommendations for Influenza, SARS and other respiratory viral test programs in China. Pg 1-26. W.H.O. Consultancy Report.

2004 Belinda Stone, Julie Burrows, Geoff Higgins, Sonia Schepetiuk, Alan Hampson, Robert Shaw and Tuckweng Kok (2004). Rapid detection and simultaneous subtype confirmation of influenza A virus by real time PCR. Journal of Virological Methods 117: 103-112.

2003 Tuckweng Kok. Suspect case of SARS in Guangdong, China Pg 1-4. (Dec 2003) World Health Organization Consultancy Report.

2002 Julie Burrows, Andreas Nitsche, Belinda Bayly, E. Walker, G. Higgins and Tuckweng Kok Detection and subtyping of Herpes simplex virus in clinical samples by LightCycler PCR, enzyme immunoassay and cell culture. BioMed Central-Microbiology 2, 12 (www.biomedcentral.com)

2001 Tuckweng Kok, Li Peng and Christopher Burrell. HIV DNA integration during cell-to-cell transmission of infection model: evidence for partially integrated structures in acutely infected cells. Archives of Virology 146: 1963-78.

2000 Tuckweng Kok, Satiya Wati, Belinda Bayly and David Devonshire-Gill Comparison of six nucleic acid extraction methods for detection of viral DNA or RNA sequences in various non-serum specimen types. J Clin Virology 16: 59-63.

1998 Tuckweng Kok. Early events in the replication cycle of human immunodeficiency virus PhD Thesis Pg.1-160, University of Adelaide.

1998 Suzana Benovic, Tuckweng Kok, A. Stephenson, J. McInnes, C. Burrell and Peng Li De novo reverse transcription of HTLV-1 RNA following cell-to-cell transmission of infection. Virology 244: 294-301.

1998 T-W Kok, P. Li and C J Burrell. Further characterization of HIV RNA synthesis early after cell-to-cell transmission infection. Archives of Virology 143: 1911-1926.

1998 Tuckweng Kok, Tom Pryor and Lyn Payne. Comparison of Rhabdomyosarcoma, Buffalo green monkey kidney epithelial, A549 (human lung epithelial) cells and human embryonic lung fibroblasts for isolation of enteroviruses from clinical samples. J Clin Virol 11: 61-65.

1998 T-W Kok, A.Sharley, L. Payne, R. B.Johnson, J. Pacina. A composite enzyme immunoassay for detection of Rotavirus and Adenovirus in faecal samples Australian J Med. Sc. 19: 56-59.

1997 Tuckweng Kok, Sonia Schepetiuk and Lance Mickan. Rapid detection, culture and typing of Herpes simplex virus. Clinical & Diagnostic Virology 10: 67-74.

1997 Sonia Schepetiuk, Tuckweng Kok, Leanne Martin, Russell Waddell and G. Higgins Detection of Chlamydia trachomatis in urine samples by nucleic acid tests with culture and enzyme immunoassay of genital samples. J Clin Microbiology 35: 3355-3357.

1997 Tuckweng Kok and Geoffrey Higgins. Prevalence of respiratory viruses and Mycoplasma pneumoniae in sputum samples from unselected adult patients. Pathology 29: 300-302.

1997 Jiang Fan, Peng Li, Tuckweng Kok and Christopher Burrell. AZT blocks down-regulation of IL-2 and IFN gene expression in HIV acutely infected cells. Archives of Virology 142:1035 - 43.

1995 Robert Norton, Sonia Schepetiuk and Tuckweng Kok. Chlamydia pneumoniae pneumonia with endocarditis. The Lancet 345: 1376-1377.

1995 S K Schepetiuk, R Norton and T W Kok (1995). Rapid diagnosis of chlamydial respiratory infection in children. Scand. J Infect. Dis. 27: 507-509.

1994 T-W Kok, L Mickan and C.J.Burrell. Routine diagnosis of seven respiratory viruses and Mycoplasma pneumoniae by enzyme immunoassay. J Virol. Meth. 50: 87-100. This publication involved in-house growth and purification of 7 different viruses and M. pneumoniae, production and characterization of various antisera as well as development of the composite enzyme immunoassay and validation with paediatric and adult specimens.

1994 J Williamson, B P Marmion, T Kok, R. Antic and R J Harris. Confirmation of fatal Mycoplasma pneumoniae infection by polymerase chain reaction detection of the adhesin gene in fixed lung tissue. J Infect. Dis. 170: 1052-53.

1994 L Mickan and T-W Kok. Recognition of adenovirus types in faecal samples by Southern hybridization in South Australia. Epidemiology and Infection 112: 603-613.

1994 P Li, A. Stephenson, L Kuiper, L. Karageorgos, T-W Kok and C. J. Burrell. Initiation of reverse transcription during cell-to-cell transmission of HIV infection uses pre-existing reverse transcriptase. J. Gen. Virol 75: 1917-1926.

1993 S K Schepetiuk, R Norton, T-W Kok and L Irving (1993). Outbreak of Adenovirus Type 4

conjunctivitis in South Australia. J. Medical Virology 41: 316-318.

1993 B P Marmion, J Williamson, T-W Kok and R J Harris. Experience with newer techniques for the laboratory detection of Mycoplasma pneumoniae infection: Adelaide, 1978-1992. Clinical Infect. Dis. 17 (Suppl): S90-99.

1993 L Mickan and T W Kok (1993). Experiences with microtiter plates and washing conditions in enzyme immunoassays. Pan American Group for Rapid Viral Diagnosis 19: 5-6.

1993 T W Kok, P Li and C J Burrell. Cell to cell transmission of human immunodeficiency virus infection induces two distinct phases of viral RNA synthesis under separate regulatory control. J Gen Virology 74: 33-38.

1993 S K Schepetiuk and Tuckweng Kok. The use of MDCK, MEK and LLC-MK2 cell lines with enzyme immunoassay for the isolation of influenza and parainfluenza viruses from clinical specimens. J Virological Methods 42: 241-250.

1993 T W Kok, L E Payne, S E Bailey, R G Waddell. Urine and the laboratory diagnosis of Chlamydia trachomatis in males. Genitourinary Medicine 69: 51-53.

1992 J W Williamson, B P Marmion, T-W Kok, G. Tannock, R. Herd and R Harris Laboratory diagnosis of Mycoplasma pneumoniae infection. 4. Antigen capture and PCR- gene amplification for detection of the mycoplasma: problems of clinical correlation Epidemiology and Infection 109: 519-537.

1990 P N Goldwater, A J Martin, T-W Kok, … and C J Burrell. A survey of nosocomial respiratory viral infections in a children’s hospital: Occult respiratory infection in patients admitted during an epidemic season. Infection Control & Hospital Epidemiology 12: 231-38.

1990 T-W Kok, K Barancek and C J Burrell. Evaluation of the Becton Dickinson Directigen test for respiratory syncytial virus in nasopharyngeal aspirates. Journal of Clinical Microbiology 28: 1458-1459.

1989 T-W Kok, B P Marmion, G Varkanis, D A Worswick and A J Martin Laboratory diagnosis of Mycoplasma pneumoniae infection. 3. Detection of IgM antibodies to M. pneumoniae by a modified indirect haemagglutination test. Epidemiology and Infection 103; 613-623.

1989 T-W Kok and C J Burrell. A comparison of five enzyme immunoassays, electron microscopy and latex agglutination tests for the detection of Rotavirus in faecal specimens Journal of Clinical Microbiology 27: 364-366.

1988 R Harris, B P Marmion, G Varkanis, T-W Kok, B Lunn and A J Martin Laboratory diagnosis of Mycoplasma pneumoniae infection. 2. Comparison of methods for the direct detection of specific antigen or nucleic acid sequences in respiratory exudates Epidemiology and Infection 101: 685-694.

1988 T-W Kok, G Varkanis, B P Marmion, J Martin and A Esterman Laboratory diagnosis of Mycoplasma pneumoniae infection. 1. Direct detection of antigen in respiratory exudates by enzyme immunoassay. Epidemiology and Infection 101: 669-684.

1987 T-W Kok. Mycoplasmas and cell cultures. Cell Focus 2: 1-4.

1986 T-W Kok. Improved laboratory diagnosis of Mycoplasma pneumoniae infection Master of Science Thesis Pg. 1-174, University of Adelaide.

1984 A R Sabato, A J Martin, B P Marmion, T-W Kok and D M Cooper. Mycoplasma pneumoniae: acute illness, antibiotics and subsequent pulmonary function. Archives of Disease in Childhood 59: 1034-1037.

Book publications

2007 Rodney Ratcliff, Grace Chang, TuckWeng Kok and Theo Sloots Molecular Diagnosis of Medical Viruses, Chapter 11, Pg. 269-298 in Molecular Diagnostics: Current Technology and Applications Eds. J R Rao, C. Fleming & J E Moore, Horizon Press Ltd.

2000 T Kok, P Li and I Gardner Applications of monoclonal antibodies in microbiology (Chapter 5, Pg. 101-126) Monoclonal Antibodies, ed H. Zola. BIOS Scientific Publishers, Oxford, United Kingdom.

1997 Tuckweng Kok and Li Peng Diagnostic Virology: specimen collection, transport and processing (Chp. 21 Pg. 115-117) Cell cultures, virus isolation and electron microscopy (Chp. 22 Pg. 119-128) Detection of viral antigens, nucleic acids and antibodies (Chp. 23 Pg. 129-140) Respiratory viruses (Chp. 25 Pg. 151-160) Arboviruses (Chp. 27 Pg. 169-174) Chlamydiae and Mycoplasmas (Chp. 29 Pg. 181-186) In Principles and Practice of Medical Laboratory Science vol. II ed. Anthony S-Y Leong. Churchill Livingstone, London.

1997 Li Peng and Tuckweng Kok Basic principles (Chp.17 Pg. 85-90) Replication (Chp.18 Pg. 91-100) Immune responses (Chp. 19 Pg. 101-108) Vaccines and antivirals (Chp. 20 Pg. 109-114) Blood-borne viruses (Chp. 24 Pg. 141-150) Contact-transmitted viruses (Chp. 26 Pg. 161-168) Enteric viruses (Chp. 28 Pg. 175-180) In Principles and Practice of Medical Laboratory Science vol. II ed. Anthony S-Y Leong. Churchill Livingstone, London.

1996 T-W Kok, D W Worswick and E. Gowans Some serological techniques for microbial and viral infections (Chapter 9: 179-204) in: Practical Medical Microbiology, ed. J. G. Collee, A. G. Fraser, B. P. Marmion and A. Simmons. 14th edition, Churchill Livingstone, London.

AWARDS

1998 Tecra Award for Best Rapid Method Satiya Wati, Tuckweng Kok, E Seah, P Wright, J Marshall and G Higgins Rapid detection of small round structured viruses (SRSVs) in faecal samples

Australian Society for Microbiology, Hobart (Sept).

1997 Australian Society for Microbiology Poster Award T-W Kok, A.Sharley, L. Payne, R. B.Johnson, J. Pacina A composite enzyme immunoassay for detection of Rotavirus and Adenovirus in faecal samples Australian Society for Microbiology Annual Conference, Adelaide (Sept).

1996 W.H.O. Influenza Award TW Kok, S Schepetiuk, L Mickan, R Shaw and A Hampson Awarded the first European Scientific Working Group on Influenza Prize at the international conference on ‘Options for the Control of Influenza III (Cairns, Australia) - World Health Organization and Australian Society for Microbiology (May).

RESEARCH & DEVELOPMENT GRANTS

2018 Influenza & Colds consultancy (Maurizio Costable & Tuckweng Kok): Kineo – City & Guilds Group, Adelaide.

2014 Haemagglutination Inhibition Assay for Influenza Antibodies ($15,000): Tuckweng Kok, Maurizio Costabile (Dr. Germain J.P. Fernando - Australian Institute for Bioengineering & Nanotechnology (AIBN), University of Queensland)

2011 Designer antigens for HIV broadly neutralizing antibody production: A tool for novel vaccine studies. P. Li, TW Kok, J. Finnie, Paul Gorry and Melissa Churchill Australian Centre for HIV & Hepatitis Virology (Awarded Dec 2011- 2014, $180,000)

2009 Australian Research Council Linkage Projects (2009-11) $500,000. Environmental Genomics: Mining, Climate Change, Water, Crime & Health. A. Cooper, D. L. Adelson, B. W. Brook, J. M. Facelli, H. B. Cross, M. I. Stevens, J. C. Paton & TW Kok.

2006 TW Kok and K. St.George. Influenza A Genomic Sequences ($3000). The Institute for Genomic Research (now part of J Craig Venter Institute). New York State Dept. of Health.

2006 Pandemic Influenza: exploiting the conserved HA cleavage site ($245000). NHMRC urgent research into avian-induced pandemic influenza. P Li and TW Kok.

2006 Rapid Point-of-Care detection of avian influenza virus using an ion channel switch biosensor NHMRC urgent research into avian-induced pandemic influenza ($424500). T W Kok, B Cornell, C J Burrell and G Higgins

2003 Influenza A & B immunochromatographic rapid tests ($35000). T W Kok, T Ziegler and Eivor Svens. Medix Biochemica, Asematie, Finland

2003 Rhinometrica Common Cold Study ($6000). Royal Adelaide Hospital. D Taverner, J Latte and T Kok.

2001 Co-operative Research Centre for sustainable aquaculture of finfish ($5000). M Crane and T W Kok. Multi-centre grant in collaboration with Australian Animal Health Laboratory Commonwealth Scientific and Industrial Research Organization, Geelong, Australia.

2001 Mycoplasmas and Articular cartilage repair. Tissue Engineering Mercy Hospital, Melbourne, Australia.

2000 Real time quantitative PCR for Influenza A & B viruses ($5000). T W Kok. Roche Molecular Diagnostics, Australia.

2000 Influenza virus culture & neuraminidase inhibitor T W Kok $20,000. R W Johnson PRI, New Jersey, USA and Jansen-Cilag, Australia.

2000 Respiratory Syncytial virus and Parainfluenza virus type III trial L Payne, T. Kok and D Roberton $14,000. Wyeth-Lederle (Australia) Pty Ltd

1999 Neuraminidase Inhibitor Ro 64-0796 (GS4104). D Worswick, W Winslow, S Schepetiuk and T Kok $25000. Roche Pharmaceuticals, Basel, Switzerland.

1999 Parainfluenza virus type 3 (cp45) vaccine trial (D125-P2). T Kok, L Payne and D Roberton $8000. Wyeth-Lederle Vaccines and Pediatrics

1997 Influenza A & B rapid tests. T-W Kok $7000. Biostar, Colorado, USA and Biota, Australia

1996 Rotavirus passive vaccine studies. R B Johnson, J Pacyna and T W Kok Numico Research Laboratories $5000, Northfield, South Australia.

1995 Poliovirus inactivation by electrolysis of sewerage samples. T W Kok $8000 Australian Water Quality, Salisbury, South Australia.

1994 Cytotoxicity from leached products. T W Kok $24000. Australian Water Quality, Salisbury, South Australia.

1993 Mycoplasma as adventitious contaminants in cell cultures T W Kok. Mercy Hospital, Melbourne and Royal Adelaide Hospital.

1992 Rapid diagnostic test for the common cold virus T-W Kok and L Mickan $20000 . Coles-Myer, Melbourne, Australia.

1991 Chlamydia confirmatory EIA for urine samples T-W Kok and R Waddell $7000. Pharmacia Biosystems, Australia.

1989 Papillomavirus expression and associated antibody response in genital infection C J Burrell, T-W Kok and G Higgins $50000. NHMRC, Australia.

1988 Clinical trial of Becton Dickinson DIRECTIGEN RSV test T-W Kok, C J Burrell and T Carski $10000. Becton-Dickinson, USA.

1988 Improved laboratory diagnosis of M. pneumoniae infection B P Marmion, R J Harris, T-W Kok and A J Martin (1988-90) $45000. NHMRC, Australia.

1985 Novel non-radioactive nucleic acid probes in the diagnosis of human non-bacterial and viral infections R H Symons, B P Marmion, C J Burrell and T Kok $10000 National Biotechnology Program, Dept. Science & Technology, Canberra, Australia.

1981 Laboratory diagnosis of M. pneumoniae infection B P Marmion, T W Kok and A J Martin $12000. Abbott Laboratories, Australia.

1977 Vibrio cholera outer membrane proteins and protection in suckling mice D Horsfall, T W Kok and D Rowley. Department of Micro & Imm, University of Adelaide.

INVITED CONSULTANCIES

2018 Influenza & Colds consultancy (Maurizio Costable & Tuckweng Kok): Kineo – City & Guilds Group, Adelaide.

2016 Visiting Professorial Fellow, Department of Microbiology, University of Kelaniya, Sri Lanka.

2014-current Editor - Journal of Virological Methods

2014 NH&MRC Australia Project Grants Review Panels invited member

2014 Public Health Virology Diagnostics, University of Medicine 1, Yangon, Myanmar

2013 NH&MRC Australia Program Grants Review Panel invited member

2012 NH&MRC Urgent Research Call into Hendra virus research project grant panel member

2012 Austrian Science Fund (July). Influenza Grant Reviewer

2010 Infectious Disease Laboratory Diagnostic Priorities Workshop (Dec 2010), China Centre for Disease Control & Prevention, Ministry of Health and W.H.O. Beijing, China

2010 Health Research Council of New Zealand (Jan and March) Grant Review Panel member for the H1N1 Rapid Response Research Initiatives

2009 National Medical Research Council of Singapore (Dec). Grants Reviews Committee

2009 NH&MRC Urgent call for H1N1 Swine Flu research. Invited member of H1N1 Influenza Review Assessment Panel

2008 Senior Advisory Group, Institute for Photonics and Advanced Sensing (IPAS), University of Adelaide.

2004 World Health Organization (Virology Consultant), Beijing, China. Influenza & Highly Pathogenic Avian Influenza, Severe Acute Respiratory Syndrome` and other respiratory viral diagnostics, surveillance and public health assessments (Feb-May).

2003 World Health Organization Virology Consultant, Beijing, China (December -January) Assessment of suspect, new case of SARS, Guangdong, China

2003 SARS Urgent Research Working Party, NHMRC Australia (July) Invited member, Committee on Assessment of SARS project grants for NH&MRC Australia.

2001 University of Indonesia Visiting virologist, Department of Microbiology, University of Indonesia, Jakarta.

1998 Numico Research Laboratory (Rotavirus vaccine), Northfield, South Australia.

1997 SA Water Quality (Pathogen Inactivation), Bolivar, South Australia. Pathogen separation and

inactivation using electrolysis.

INVITED PRESENTATIONS

2015 Intracellular inhibition of Influenza A replication by anti-viral IgA 11th Australian Influenza Symposium (W.H.O.) Geelong, Victoria (October)

2010 The Past Prepares for The Future W.H.O. Workshops: Laboratory Network of Emergency Response and Surveillance of Infectious Diseases, Guangzhou (Dec 11-14) & Beijing (Dec 15-17), China.

2010 Influenza – The Infection and its Control 100 Years of Tropical Medicine Conference James Cook University, Townsville (June)

2010 Mycoplasmas as adventitious agents in cell cultures International Society for Cellular Therapy Australia-China-Japan Biotherapeutic Symposium (April)

2009 Laboratory Diagnosis of Viral Infections: Back to the Future 8th Asia Pacific Congress of Medical Virology, Hong Kong (Feb)

2007 Respiratory Viral Infections Postgraduates Virology Master Class, University of Adelaide (June 2006 & 2007)

2006 Rapid detection of Influenza using an ion-channel switch biosensor First National Influenza Symposium, Canberra (2006)

2004 The Joys of Virus Hunting Capital Institute of Pediatrics, Chao Yang District, Beijing (Feb)

2004 Respiratory Viral Infections World Bank-Ministry of Health, SARS & Influenza Workshop, Beijing (Mar)

2004 Laboratory Diagnosis of Influenza Training Workshop - Human and Avian Influenza, Sino-Danish Centre, Beijing (Mar)

2002 Viruses and Products in Blood Gambro Blood Component Technology Centre, Denver, Colorado.

2001 Virology – Back to Basics Departments of Immunology and Microbiology, Chinese University of Hong Kong (Dec)

2001 Viruses – Detection and Epidemiology Naval Health Research Centre US Navy, San Diego, California (July)

2001 Virus Detection - Traditional to Current Molecular Approaches Singapore Society for Microbiology & Biotechnology, Singapore (July)

2001 Cytomegalovirus: Culture to Molecular Diagnosis – End Organ Disease Department of Microbiology, University of Indonesia, Jakarta (May)

2001 Virus Detection - Traditional to Current Molecular Approaches Department of Microbiology, University of Indonesia, Jakarta (May)

1999 Recombinant phage display Fab antibody libraries to Bacillus anthracis, Yersinia pestis and Q fever. Australian Membrane and Biotechnology Research Institute, Co-operative Research Centre Molecular Engineering & Technology, Sydney, Australia (Feb)

1998 Virus Detection - A cocktail of strategies. Australian Society for Microbiology, Hobart, Australia (July)

1998 The state of clinical and diagnostic virology Australian Society for Microbiology, Hobart, Australia (July)

1998 Improved rapid detection of measles virus Standardization of the nomenclature for genetic characteristics of wild-type measles virus WHO Geneva, Switzerland (July).

1998 HIV RNA transcription patterns Institute of Veterinary Virology, University of Bern, Switzerland (July)

1996 Laboratory diagnosis of Chlamydia trachomatis infection by nucleic acid tests, enzyme immunoassay and culture with urine and genital samples Australian Institute of Medical Scientists Annual Convention, Adelaide, Australia (July)

1994 Experiences with rapid diagnosis of human rotavirus in Australia. Dakopatts International Conference on Rapid Diagnosis of Infectious Diseases, Santa Barbara, California (Sept)

1994 HIV RNA synthesis and DNA integration kinetics (1994) Department of Haematology-Oncology, University of California, Los Angeles (Sept)

1993 The use of enzyme immunoassays for diagnosis of viral and chlamydial infections ELISA National Conference, Dakopatts, Sydney, Australia (Aug)

1991 Rapid virus diagnosis and enzyme immunoassay (1991) Rapid Virus Diagnosis Symposium, Australian Society for Microbiology, Queensland, Australia (July)

1990 Rapid diagnosis of respiratory viral and mycoplasmal infections Department of Virology, University of Turku, Finland (Oct)

1987 Laboratory diagnosis of Mycoplasma pneumoniae infection Department of Virology, University of Turku, Finland (Sept)

TEACHING (Lectures and Tutorials)

University of Adelaide Infection & Immunity – Respiratory Viruses and Diagnostic Virology (2000 – 2013) Bachelor of Science, B. Biomedical Science, B. Biotechnology and M.B.B.S.

University of South Australia Comprehensive Virology course – 14 lectures p.a. (2000 – 2013) Classical to Molecular Virology and Viral Pathogenesis Bachelor of Laboratory Medicine, B. Biotechnology and B. Medical Science

University of Adelaide and Royal College of Pathologists Australasia Initiated Virology Master Class Postgraduate Course and Convener (2006-2010) Viral Diagnostics & Public Health for Medical Microbiol. registrars training for Fellow of RCPA.

PROFESSIONAL SOCIETY/COMMITTEE APPOINTMENTS

International 2008-2011 Scientific Advisory Council, International Congress of Virology, International Union of Microbiological Societies 2008-2012 Scientific Advisory Council member, Asia Pacific Congress of Medical Virology 2012 Asia Pacific Congress of Med. Virology Dep. Chair, Adelaide.

National

2017 Principal Examiner (Microbiology) (RCPA) 2015-16 Dep. Principal Examiner (Microbiology) Royal College of Pathologists Australasia(RCPA) 2012-14 Chairman - Institutional Biosafety Committee (Genetic Manipulation and Biocontainment) 2011 Royal College of Pathologists Australasia, Faculty of Science Examiners 2010-2017 National Examinations & Qualifications Board, Australian Soc. Microbiology (Registrar) 2010 Physical Containment Levels 2 & 3 Facilities Manager (IMVS and SA Pathology) 2009 National Task Force, Australian Society for Microbiology 2009 In vitro Medical Device Advisory Committee, Therapeutic Goods Administration, Australia. 2006 IMVS - Institute Biosafety Committee member. 2005 National Scientific Advisory-Virology Chair, Australian Society Microbiology. 2002 Chairman (2002) & Treasurer (2001) of South Australian Medical Scientists Association. 2000 IMVS & RAH Medical Scientist Assessment Committee member 1999 Corporate OH & S committee – Garvan Inst. Med. Research, Sydney.

PATENT

Immunogenic polypeptides derived from the cleavage loop of precursor hemagglutinin of an influenza virus (Peng Li, Tuckweng Kok and Darren Miller) PCT International Application 3695PCT (April 2007) Australia

LABORATORY SKILLS

Comprehensive working knowledge and experience with the following skills:

Viral and Cell Biology Viral assays and Serology (Haemagglutination Inhibition, Plaque Neutralizations, Viral quantal assays - TCID50, etc.).

Purifications of >12 virus types (PC2 and PC3 containments) Virus cultures and identification of specific cytopathic effects Suckling mice models for viruses and bacteria (Salmonella and E.coli serotyping) Virus inoculations in embryonated chicken eggs. Cell and tissue cultures (primary, continuous and explants) Mitochondria purification

Antibody Technologies Monoclonal and polyclonal antibody productions Enzyme immunoassays, Immunofluorescence, Immunohistochemistry Crossed immune-electrophoresis Immune Electron Microscopy Phage display recombinant Fab antibody libraries

Molecular and Recombinant Biology Southern and Northern blot hybridizations PCR, Molecular Cloning, Sequence Analysis Human papillomavirus (Types 6, 11, 16 & 18) antigens in E.coli expression system

Certifications Office of the Gene Technology Regulator (OGTR Australia) – Genetic Manipulation

Security Sensitive Biological Agents and Quarantine Approved Premises, Department of Agriculture, Australia.

W.H.O., WPRO Consultancy Field Safety, Manila, Philippines.

MICROBIOLOGY SOCIETIES

Member of the Society for General Microbiology Member of the American Society for Virology Life Member of the Pan American Society for Clinical Virology Life Member of the Asia Pacific Society for Medical Virology

OTHER PERSONAL DETAILS

Citizenship: Australian Country of Birth: Malaysia Languages: English & Cantonese (native), Malay (fluent), Mandarin (conversational) Secondary School: Anglo Chinese School, Ipoh, Malaysia. Primary School: Anglo Chinese School, Kampar, Malaysia.

COMMUNITY SERVICE

2015 President Rotary Club St. Peters, South Australia 2014 Rotary International, Paul Harris Fellow 2012 National Youth Science Foundation, Canberra, Rotary District 9520 Chairman 2006 Treasurer - Rotary Club of St. Peters, South Australia

------o------