DOCSLIB.ORG

Author's Personal Copy Montanoa Tomentosa Glandular Trichomes

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Author's Personal Copy Montanoa Tomentosa Glandular Trichomes Author's personal copy Fitoterapia 80 (2009) 12–17 Contents lists available at ScienceDirect Fitoterapia journal homepage: www.elsevier.com/locate/fitote Montanoa tomentosa glandular trichomes containing kaurenoic acids chemical profile and distribution Ramón E. Robles-Zepeda a, Edmundo Lozoya-Gloria b, Mercedes G. López c, María L. Villarreal d, Enrique Ramírez-Chávez c, Jorge Molina-Torres c,⁎ a Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, Mexico b Departamento de Ingeniería Genética, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Guanajuato, Mexico c Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Irapuato, Guanajuato, Mexico d Departamento de Productos Naturales, Centro de Investigación en Biotecnología, UAEM, Cuernavaca, Morelos, Mexico article info abstract Article history: Montanoa tomentosa has been used in traditional medicine in Mexico to treat diverse female Received 20 August 2008 health disorders; it is particularly useful in inducing childbirth. Microscopic analysis of leaf Accepted in revised form 3 September 2008 surfaces of M. tomentosa revealed the presence of glandular trichomes. The chemical profile Available online 14 September 2008 and distribution of glandular trichomes from different developmental stages of M. tomentosa leaves were investigated. Two diterpenic acids, kaurenoic and grandiflorenic were detected in Keywords: glandular trichomes through the glandular microsampling technique and GC/MS analysis. In Montanoa tomentosa the glandular trichomes of the leaves also up to twenty-six volatile terpenes were identified, Asteraceae β Zoapatle where -eudesmol and valencene were the most abundant terpenes. Glandular trichomes © 2008 Elsevier B.V. All rights reserved. Volatile terpenes Kaurenoic acid Grandiflorenic acid 1. Introduction of their exudates throughout leaf development may have important consequences for plant adaptation to abiotic and The aerial surface of most plants contains trichomes, which biotic factors [6]. can be divided into glandular (GT) and non-glandular (NGT). Montanoa tomentosa Cerv. (Asteraceae), a perennial shrub GTs are secretory structures varying in size, form, location, known as zoapatle or cihuapatli, has been used in traditional and function in plants of different species. Peltate and capitate medicine in Mexico to treat diverse female health disorders glandular trichomes are common in plants [1] and are [7,8], as for example inducing childbirth [9] and some authors constituted of four to eight cells in a single disc [2]. Glandular attribute theses pharmacological properties of M. tomentosa trichomes have a spherical morphology after reaching the to two diterpenic acids: grandiflorenic (GA) [kaura-9 (11), 16- mature stage. Their characteristic morphology develops as a dien-18-oic acid] and kaurenoic (KA) [kaur-16-en-18-oic acid] result of the accumulation of secretory products [3]. They [10]. These diterpenic acids are present in other plants and contain chemical compounds with diverse functions, includ- have been fully chemically characterized previously [11,12] ing defense [4]. Secondary metabolites in trichomes are (Fig. 1). ecologically bioactive compounds of interest as pesticides, Stereomicroscopic observation of the surface of leaves of pharmaceuticals, flavors, and fragrances [5]. Changes in M. tomentosa confirmed the presence of abundant glandular number of trichomes and in composition and concentrations trichomes. In the present work, we studied the distribu- tion and chemical profiles of glandular trichomes of leaves of M. tomentosa at different development stages. Emphasis is ⁎ Corresponding author. Tel.: +52 462 6239 645; fax: +52 462 6245 996. put in the presence of kaurenoic acids, putative bioactive com- E-mail address: [email protected] (J. Molina-Torres). ponents responsible for the ethnomedicinal use of this species. 0367-326X/$ – see front matter © 2008 Elsevier B.V. All rights reserved. doi:10.1016/j.fitote.2008.09.002 Author's personal copy R.E. Robles-Zepeda et al. / Fitoterapia 80 (2009) 12–17 13 2. Experimental from about 700 glands, was dissolved in 500 μl of hexane: ethyl acetate (85:15). The solvent was evaporated to dryness 2.1. Plant under nitrogen and the residue re-suspended in 100 μlof ethanol. Samples were kept at 4 °C for further analysis. M. tomentosa leaves were collected from field-grown mature one-year-old plants cultivated at CINVESTAV-IPN, 2.4. Glandular trichomes terpenes content Unidad Irapuato (N 20° 43′ 8.9″, W 101° 19′ 42.6″ altitude 1740 m), Guanajuato, Mexico. Samples were collected form A more general procedure was used to evaluate the September to December after the rainy season. Dr. Jerzy terpenes content of the GTs. First, a surface extraction was Rzedowski from the Instituto de Ecología, Centro Regional del carried out with dichloromethane as previously described Bajío in Pátzcuaro, Michoacán, México performed species [13]. Briefly, each leaf section used for determination of GTS authentication. A voucher specimen (IEB-57689) was depos- density was subsequently submerged in 20 ml dichloro- ited in this Institute. methane for 20 s. Then, the solvent was evaporated under a nitrogen stream and the residue re-suspended in 1 ml of the 2.2. Leaf glandular trichomes distribution same solvent and kept at 4 °C for further analysis. Leaves were collected in four size ranges: 7 to 7.5 cm, 9 to 2.4.1. Grandiflorenic and kaurenoic acids evaluation 9.5 cm, 14 to 14.5 cm, and 15 to 15.5 cm. These were classified The identification and quantification of these acids was as new, young, mature and old leaves, respectively. Within accomplished as follows: 100 μl of each surface extract was these size ranges, the smaller leaves were found in a higher dissolved in hexane:ethyl acetate (85:15), and the diterpenic position in the plant, whereas the older leaves were found at acids present derivatized adding100 μl of a mixture of BSTFA lower level. In order to evaluate the glandular trichomes (GT) [bis-(trimethyl) sililtrifluoroacetamide from Sigma Co.] and distribution density, each leaf was divided in three zones TMCS (trimethylchlorosilane from Alltech) in a 100:5 v/v characterized as basal, middle and apical. relation and heating for 30 min at 60 °C. The derivatized The evaluation of GTs density in the different leaf zones compounds were analyzed using a gas chromatograph was performed with the aid of a stereomicroscope (Nikon coupled to a mass selective detector (GC-MSD Hewlett- SMZ-2B, Schott KL500 lamp). The average number of GTs was Packard 6890 Series II) equipped with a capillary column calculated. Three replicas were evaluated for each leaf size. HP-1 (30 m long, 0.25 mm internal diameter and 0.25 μm film thickness). Helium was used as the carrier gas, at a 2.3. Glandular trichomes microsampling constant flow rate of 1 ml/min. The injector temperature was maintained at 200 °C. The oven temperature profile was the Three replicas of GTs direct extraction (microsampling) following: 3 min at 150 °C, followed by a temperature were performed with micro capillaries (prepared from 10 μl increment rate of 4 °C/min up to 300 °C, then kept at 300 °C capillary tubes by heat stretching) under the stereomicro- for 20 min. The mass selective detector consisted of a scope at a 100x magnification. The glandular fluid collected quadrupole analyzer with the ionization source set at 70 eV. Scan parameters were: mass range from 30.0 to 550 uam with 5.36 scans per second, and the temperatures of source and quadrupole were 230 °C and 150 °C respectively. The mass spectra identification and calibration curves for these com- pounds were made by comparison with authenticated standards obtained as a gift from Dr. Raúl Enríquez (Instituto de Química, UNAM, México). 2.4.2. Terpenic compounds profile The GTs dichloromethane extract of basal, middle, and apical zones, from leaves of different sizes were analyzed by GC-MSD. One microliter of each extract was injected onto a GC (Hewlett-Packard 5890 series II) coupled to a mass selective quadrupole detector (Hewlett-Packard/MSD 5972 series) for the characterization of components present in the samples. Compound separation was carried out using a capillary column HP-FFAP (25 m long, 0.32 mm internal diameter and 0.52 μm film thickness from Hewlett-Packard). The carrier gas was helium at a constant flow rate of 1 ml/min. The in- jector temperature was maintained at 180 °C. The oven temperature program was: 3 min at 40 °C, followed by a temperature increment rate of 3 °C/min up to 120 °C and then at a rate of 5 °C/min up to 200 °C. This temperature was maintained for 13 min. Peaks were identified by comparison Fig. 1. Structure of diterpenic acids in this study: A) Kaurenoic acid; with the NIST 98 mass spectral database. Results are presented B) Grandiflorenic acid. as a percent of the total area produced by the compounds Author's personal copy 14 R.E. Robles-Zepeda et al. / Fitoterapia 80 (2009) 12–17 identified in each leaf zone. The values presented are the aver- age of three replicas. 3. Results and discussion 3.1. Leaf glandular trichomes distribution The abaxial surface presented numerous GTs and Non- Glandular trichomes (NGT). On the adaxial surface only NGTs were observed. Based on observations using a stereomicro- scope, the GTs were distributed on the leaf lamina, on sec- ondary, tertiary, and quaternary veins with the exception of the main vein, where only NGTs were observed. The GTs have a green-yellowish color and a spherical shape. NGTs in both surfaces were multicellular and erect varying in length. As the GTs were restricted to the abaxial side of the leaf, the results presented here on the density of GTs are exclusively related to the abaxial surface of the leaves. GTs density did not vary significantly between different zones of the new, mature, and old leaves, but in young leaves, a higher GTs density was observed throughout this surface, as shown in the graph presented in Fig.
Load more

Recommended publications
  • Volatile Organic Compounds of Leaves and Flowers of Montanoa Tomentosa
    FLAVOUR AND FRAGRANCE JOURNAL VOLATILE OILS OF MONTANOA TOMENTOSA Flavour Fragr. J. (In press) Published online in Wiley InterScience (www.interscience.wiley.com). DOI: 10.1002/ffj.1560 Volatile organic compounds of leaves and flowers of Montanoa tomentosa Ramón Enrique Robles-Zepeda,1 Jorge Molina-Torres,2 Edmundo Lozoya-Gloria2 and Mercedes G. López2* 1 Universidad de Sonora, Encinas y Rosales, 83000, Hermosillo, Sonora, México 2 Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, 36500 Irapuato, Guanajuato, México Received 15 January 2004; Revised 7 September 2004; Accepted 4 October 2004 ABSTRACT: Montanoa tomentosa is a valued plant due to its medicinal properties, mainly as a menstruation and child- birth inducer. The volatile constituents of the aerial parts (leaves and flowers) of this medicinal plant were analysed by SPME–GC–MS. The major constituents of the volatile fraction were monoterpenes, such sabinene, α-pinene and α- thujene, which accounted for 65% of the total fraction. Other identified compounds were sesquiterpenes, like α-gurjunene, caryophyllene and germacrene D, but in minor amount. The total proportion of identified compounds was 86.7% (17 volatiles) and 82.1% (14 volatiles) for leaves and flowers, respectively. Some of the most abundant terpenes found in this plant are well known for their relevant roles in the environment and in many industrial applications. The characteriza- tion of volatile from M. tomentosa is established in this study for the first time, therefore their utility in different aspects, opens an interesting area to carry out further investigation of the plant. Copyright © 2005 John Wiley & Sons, Ltd.
    [Show full text]
  • ABSTRACTS 117 Systematics Section, BSA / ASPT / IOPB
    Systematics Section, BSA / ASPT / IOPB 466 HARDY, CHRISTOPHER R.1,2*, JERROLD I DAVIS1, breeding system. This effectively reproductively isolates the species. ROBERT B. FADEN3, AND DENNIS W. STEVENSON1,2 Previous studies have provided extensive genetic, phylogenetic and 1Bailey Hortorium, Cornell University, Ithaca, NY 14853; 2New York natural selection data which allow for a rare opportunity to now Botanical Garden, Bronx, NY 10458; 3Dept. of Botany, National study and interpret ontogenetic changes as sources of evolutionary Museum of Natural History, Smithsonian Institution, Washington, novelties in floral form. Three populations of M. cardinalis and four DC 20560 populations of M. lewisii (representing both described races) were studied from initiation of floral apex to anthesis using SEM and light Phylogenetics of Cochliostema, Geogenanthus, and microscopy. Allometric analyses were conducted on data derived an undescribed genus (Commelinaceae) using from floral organs. Sympatric populations of the species from morphology and DNA sequence data from 26S, 5S- Yosemite National Park were compared. Calyces of M. lewisii initi- NTS, rbcL, and trnL-F loci ate later than those of M. cardinalis relative to the inner whorls, and sepals are taller and more acute. Relative times of initiation of phylogenetic study was conducted on a group of three small petals, sepals and pistil are similar in both species. Petal shapes dif- genera of neotropical Commelinaceae that exhibit a variety fer between species throughout development. Corolla aperture of unusual floral morphologies and habits. Morphological A shape becomes dorso-ventrally narrow during development of M. characters and DNA sequence data from plastid (rbcL, trnL-F) and lewisii, and laterally narrow in M.
    [Show full text]
  • Everywhere but Antarctica: Using a Super Tree to Understand the Diversity and Distribution of the Compositae
    BS 55 343 Everywhere but Antarctica: Using a super tree to understand the diversity and distribution of the Compositae VICKI A. FUNK, RANDALL J. BAYER, STERLING KEELEY, RAYMUND CHAN, LINDA WATSON, BIRGIT GEMEINHOLZER, EDWARD SCHILLING, JOSE L. PANERO, BRUCE G. BALDWIN, NURIA GARCIA-JACAS, ALFONSO SUSANNA AND ROBERT K. JANSEN FUNK, VA., BAYER, R.J., KEELEY, S., CHAN, R., WATSON, L, GEMEINHOLZER, B., SCHILLING, E., PANERO, J.L., BALDWIN, B.G., GARCIA-JACAS, N., SUSANNA, A. &JANSEN, R.K 2005. Everywhere but Antarctica: Using a supertree to understand the diversity and distribution of the Compositae. Biol. Skr. 55: 343-374. ISSN 0366-3612. ISBN 87-7304-304-4. One of every 10 flowering plant species is in the family Compositae. With ca. 24,000-30,000 species in 1600-1700 genera and a distribution that is global except for Antarctica, it is the most diverse of all plant families. Although clearly mouophyletic, there is a great deal of diversity among the members: habit varies from annual and perennial herbs to shrubs, vines, or trees, and species grow in nearly every type of habitat from lowland forests to the high alpine fell fields, though they are most common in open areas. Some are well-known weeds, but most species have restricted distributions, and members of this family are often important components of 'at risk' habitats as in the Cape Floral Kingdom or the Hawaiian Islands. The sub-familial classification and ideas about major patterns of evolution and diversification within the family remained largely unchanged from Beutham through Cronquist. Recently obtained data, both morphologi- cal and molecular, have allowed us to examine the distribution and evolution of the family in a way that was never before possible.
    [Show full text]
  • TPG Index Volumes 1-35 1986-2020
    Public Garden Index – Volumes 1-35 (1986 – 2020) #Giving Tuesday. HOW DOES YOUR GARDEN About This Issue (continued) GROW ? Swift 31 (3): 25 Dobbs, Madeline (continued) #givingTuesday fundraising 31 (3): 25 Public garden management: Read all #landscapechat about it! 26 (W): 5–6 Corona Tools 27 (W): 8 Rocket science leadership. Interview green industry 27 (W): 8 with Elachi 23 (1): 24–26 social media 27 (W): 8 Unmask your garden heroes: Taking a ValleyCrest Landscape Companies 27 (W): 8 closer look at earned revenue. #landscapechat: Fostering green industry 25 (2): 5–6 communication, one tweet at a time. Donnelly, Gerard T. Trees: Backbone of Kaufman 27 (W): 8 the garden 6 (1): 6 Dosmann, Michael S. Sustaining plant collections: Are we? 23 (3/4): 7–9 AABGA (American Association of Downie, Alex. Information management Botanical Gardens and Arboreta) See 8 (4): 6 American Public Gardens Association Eberbach, Catherine. Educators without AABGA: The first fifty years. Interview by borders 22 (1): 5–6 Sullivan. Ching, Creech, Lighty, Mathias, Eirhart, Linda. Plant collections in historic McClintock, Mulligan, Oppe, Taylor, landscapes 28 (4): 4–5 Voight, Widmoyer, and Wyman 5 (4): 8–12 Elias, Thomas S. Botany and botanical AABGA annual conference in Essential gardens 6 (3): 6 resources for garden directors. Olin Folsom, James P. Communication 19 (1): 7 17 (1): 12 Rediscovering the Ranch 23 (2): 7–9 AAM See American Association of Museums Water management 5 (3): 6 AAM accreditation is for gardens! SPECIAL Galbraith, David A. Another look at REPORT. Taylor, Hart, Williams, and Lowe invasives 17 (4): 7 15 (3): 3–11 Greenstein, Susan T.
    [Show full text]
  • Taxonomy and Floristics / Taxonomía Y Florística
    Botanical Sciences 99(3): 708-716. 2021 Received: September 19, 2020, Accepted: January 13, 2021 DOI: 10.17129/botsci.2754 DichrocephalaOn integrifolia line first: April in Mexico15, 2021 Taxonomy and Floristics / Taxonomía y Florística DICHROCEPHALA INTEGRIFOLIA (ASTEREAE, ASTERACEAE), A NEW EXOTIC GENUS AND SPECIES FOR MEXICO AND SECOND RECORD FOR THE NEW WORLD DICHROCEPHALA INTEGRIFOLIA (ASTEREAE, ASTERACEAE), UN GÉNERO Y ESPECIE EXÓTICOS NUEVOS PARA MÉXICO Y SEGUNDO REGISTRO PARA EL NUEVO MUNDO OSCAR HINOJOSA-ESPINOSA1*, DANIEL POTTER1, MARIO ISHIKI2, ENRIQUE ORTIZ3, AND JOSÉ LUIS VILLASEÑOR3 1 Department of Plant Sciences, University of California, Davis, California, USA. 2 El Colegio de la Frontera Sur, Unidad San Cristóbal, San Cristóbal de Las Casas, Chiapas, México. 3 Departamento de Botánica, Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México, México *Author for correspondence: [email protected] Abstract Background: Dichrocephala is an Old-World genus of the tribe Astereae within the family Asteraceae. One species, D. integrifolia, has been recently reported as introduced in the New World from a pair of collections from Guatemala. During field work in the state of Chiapas in south- ern Mexico, the species was found and collected. This is the first record of both the genus and species in Mexico and the second record for these taxa in the Americas. Question: Can D. integrifolia occur in more areas in the New World besides those known from Guatemala and Chiapas? Studied species: Dichrocephala integrifolia Study site: Mexico, Central America, and the Caribbean. Methods: An ecological niche model was made and it was projected into the New World. Results: The ecological niche model predicts the records of D.
    [Show full text]
  • Taxonomic Studies of the Genus Polymnia L
    This dissertation has been 64—6976 microfilmed exactly as received WELLS, James Ray, 1932— TAXONOMIC STUDIES OF THE GENUS POLYMNIA L. The Ohio State University, Ph.D., 1963 Botany University Microfilms, Inc., Ann Arbor, Michigan TAXONOMIC STUDIES OF THE GENUS POLYMNIA L. DISSERTATION Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By JAMES RAY WELLS, B.S., M.S. The Ohio State University 1963 Approved by Adviser Department of Botany and Plant Pathology ACKNOWLEDGMENTS I wish to express my sincere gratitude to Dr. T. v Richard Fisher, my adviser, who suggested this problem and under whose guidance the work wan carried out. I am also grateful to Drs. Clara Weishaupt, J.W.A. Burley, C. E. Taft, and Glenn W. Blaydes for reading this dissertation and for offering helpful suggestions and criticisms. I am indebted to the curators of the several herbaria for making their material available. Permission to use Goode Base Maps (Copyright by the University of Chicago) for plotting plant distributions is gratefully acknowledged. This permission was granted through Goode Base Map Series, Department of Geography, The University of Chicago. My wife Jan, is due special recognition for her loyal support and cooperation throughout this endeavor. TABLE OF CONTENTS Page ACKNOWLEDGMENTS....................................... ii LIST OF T A B L E S ........................................ iv LIST OF ILLUSTRATIONS................................ v INTRODUCTION ........................................... 1 MORPHOLOGY ............................................. 3 GEOGRAPHIC DISTRIBUTION .............................. 5 ECONOMIC IMPORTANCE . .............................. 7 TAXONOMY ............................................... 8 KEY TO SPECIES AND VARIETIES.......................... 11 DESCRIPTION AND DISCUSSION OF T A X A .................... 15 EXPERIMENTAL TAXONOMIC INVESTIGATIONS ...............
    [Show full text]
  • Generating and Filtering Major Phenotypic Novelties: Neogoldschmidtian Saltation Revisited
    Chapter 7 Generating and filtering major phenotypic novelties: neoGoldschmidtian saltation revisited Richard N\. Bateman and William A. D/M/che/e ABSTRACT Further developing a controversial neoGoldschmidtian paradigm that we first pub- lished in 1994, we here narrowly define saltational evolution as a genetic modifica- tion that is expressed as a profound phenotypic change across a single generation and results in a potentially independent evolutionary lineage termed a prospecies (the 'hopeful monster' of Richard Goldschmidt). Of several saltational and parasaltational mechanisms previously discussed by us, the most directly relevant to evolutionary-developmental genetics is dichotomous saltation, which is driven by mutation within a single ancestral Uneage. It can result not only in instantaneous speciation but also in the simultaneous origin of a profound phenotypic novelty more likely to be treated as a new supraspecific taxon. Saltational events form unusually long branches on morphological phylogenies, which follow a punctuated equilibrium pattern, but at the time of origin they typically form zero length branches on the contrastingly gradualistic molecular phylogenies. Our chosen case- studies of heterotopy (including homeosis) and heterochrony in fossil seed-ferns and extant orchids indicate that vast numbers of prospecies are continuously generated by mutation of key developmental genes that control morphogenesis. First principles suggest that, although higher plant mutants are more likely to become established than higher animals, the fitness of even plant prospecies is in at least most cases too low to survive competition-mediated selection. The establishment of prospecies is most Hkely under temporary release from selection in environments of low biotic competition for resources, followed by honing to competitive fitness by gradual rein- troduction to neoDarwinian selection.
    [Show full text]
  • Montanoa Tomentosa
    Latin American Journal of Pharmacy Regular Article (formerly Acta Farmacéutica Bonaerense) Received: May 6, 2011 Revised version: May 9, 2011 Lat. Am. J. Pharm. 30 (7): 1366-71 (2011) Accepted: May 17, 2011 Accumulation and Distribution of Diterpenic Acids in Leaves of Montanoa tomentosa Ramón E. ROBLES-ZEPEDA 1, Edmundo LOZOYA-GLORIA 2, Mercedes G. LÓPEZ 3, & Jorge MOLINA-TORRES *3 1 Departamento de Ciencias Químico Biológicas, Universidad de Sonora, Hermosillo, Sonora, México. 2 Departamento de Ingeniería Genética, 3 Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Km. 9.6 Libramiento Norte, Irapuato, Guanajuato, México. SUMMARY. Montanoa tomentosa has been used for at least last five centuries in traditional medicine in Mexico as a remedy for reproductive impairments. The accumulation of diterpenic acids in M. tomentosa leaves was determined. Using Scanning Electron Microscopy, the type and distribution of glandular tri- chomes (GTs) was observed on the abaxial and adaxial sides of leaves. GTs and non-glandular trichomes (NGTs) were observed on leaf surface, but the latter are confined to the leaves abaxial side. On the adaxial surface, only non-glandular trichomes were observed. Accumulation in GTs and leaf lamina of kaurenoic (KA) and grandiflorenic (GFA) acids was determined by Gas Chromatography coupled to an Electron Im- pact Mass Spectrometric Detector (GC/EI-MSD). GC-MSD analysis indicated that GTs accumulated KA and GFA, with KA accumulation being at a higher level than GFA in these structures. Attention on GFA and KA is due to their importance as plant growth regulator precursors with potential pharmacological applications.
    [Show full text]
  • Chemical Composition and Antioxidant DPPH Activity of the Floral and Leaves Essential Oils of Montanoa Speciosa DC
    American Journal of Plant Sciences, 2017, 8, 745-753 http://www.scirp.org/journal/ajps ISSN Online: 2158-2750 ISSN Print: 2158-2742 Chemical Composition and Antioxidant DPPH Activity of the Floral and Leaves Essential Oils of Montanoa speciosa DC Durcy Ruiz-Ciau1, Luis Cuevas-Glory2, Leovigildo Quijano3, Enrique Sauri-Duch2 1Facultad de Química, Universidad Autónoma de Yucatán, Mérida, México 2División de Estudios de Posgrado e Investigación, Departamento de Ingeniería Química y Bioquímica del Instituto Tecnológico de Mérida, Tecnológico Nacional de México, Km. 5 Carr. Mérida-Progreso, Mérida, México 3Facultad de Química, Universidad Nacional Autónoma de México, Mérida, México How to cite this paper: Ruiz-Ciau, D., Abstract Cuevas-Glory, L., Quijano, L. and Sauri- Duch, E. (2017) Chemical Composition The essential oils obtained by hydrodistillation from leaves and flowers of and Antioxidant DPPH Activity of the Montanoa speciosa collected in southeastern Mexico (Yucatan) were analyzed Floral and Leaves Essential Oils of Monta- by GC-MS. A total of 71 and 79 components, representing 98.44% and 97.69% noa speciosa DC. American Journal of Plant Sciences, 8, 745-753. of the leaf and flower oils, respectively, were characterized. The main consti- https://doi.org/10.4236/ajps.2017.84052 tuents found were β-caryophyllene (20.73%, 17.95%), δ-cadinene (9.88%, 9.28%), caryophyllene oxide (9.48%, 8.68%), and germacrene D (6.94%, 5.85%). The Received: April 28, 2016 essential oils were screened for their antioxidant potentials by DPPH assay. Accepted: March 17, 2017 Published: March 20, 2017 The leaves oil exhibited higher DPPH scavenging capability (72.85 ± 0.28 mmol TE/g essential oil and 147.83 ± 0.41 mg/mL Vit C/g essential oil) than Copyright © 2017 by authors and the floral oil (68.43 ± 0.10 mmol TE/g essential oil and 131.59 ± 0.87 mg/mL Scientific Research Publishing Inc.
    [Show full text]
  • The Leipzig Catalogue of Plants (LCVP) ‐ an Improved Taxonomic Reference List for All Known Vascular Plants
    Freiberg et al: The Leipzig Catalogue of Plants (LCVP) ‐ An improved taxonomic reference list for all known vascular plants Supplementary file 3: Literature used to compile LCVP ordered by plant families 1 Acanthaceae AROLLA, RAJENDER GOUD; CHERUKUPALLI, NEERAJA; KHAREEDU, VENKATESWARA RAO; VUDEM, DASHAVANTHA REDDY (2015): DNA barcoding and haplotyping in different Species of Andrographis. In: Biochemical Systematics and Ecology 62, p. 91–97. DOI: 10.1016/j.bse.2015.08.001. BORG, AGNETA JULIA; MCDADE, LUCINDA A.; SCHÖNENBERGER, JÜRGEN (2008): Molecular Phylogenetics and morphological Evolution of Thunbergioideae (Acanthaceae). In: Taxon 57 (3), p. 811–822. DOI: 10.1002/tax.573012. CARINE, MARK A.; SCOTLAND, ROBERT W. (2002): Classification of Strobilanthinae (Acanthaceae): Trying to Classify the Unclassifiable? In: Taxon 51 (2), p. 259–279. DOI: 10.2307/1554926. CÔRTES, ANA LUIZA A.; DANIEL, THOMAS F.; RAPINI, ALESSANDRO (2016): Taxonomic Revision of the Genus Schaueria (Acanthaceae). In: Plant Systematics and Evolution 302 (7), p. 819–851. DOI: 10.1007/s00606-016-1301-y. CÔRTES, ANA LUIZA A.; RAPINI, ALESSANDRO; DANIEL, THOMAS F. (2015): The Tetramerium Lineage (Acanthaceae: Justicieae) does not support the Pleistocene Arc Hypothesis for South American seasonally dry Forests. In: American Journal of Botany 102 (6), p. 992–1007. DOI: 10.3732/ajb.1400558. DANIEL, THOMAS F.; MCDADE, LUCINDA A. (2014): Nelsonioideae (Lamiales: Acanthaceae): Revision of Genera and Catalog of Species. In: Aliso 32 (1), p. 1–45. DOI: 10.5642/aliso.20143201.02. EZCURRA, CECILIA (2002): El Género Justicia (Acanthaceae) en Sudamérica Austral. In: Annals of the Missouri Botanical Garden 89, p. 225–280. FISHER, AMANDA E.; MCDADE, LUCINDA A.; KIEL, CARRIE A.; KHOSHRAVESH, ROXANNE; JOHNSON, MELISSA A.; STATA, MATT ET AL.
    [Show full text]
  • The Asteraceae of Northwestern Pico Zunil, a Cloud Forest in Western Guatemala
    NUMBER 10 QUEDENSLEY AND BRAGG: ASTERACEAE OF PICO ZUNIL, GUATEMALA 49 THE ASTERACEAE OF NORTHWESTERN PICO ZUNIL, A CLOUD FOREST IN WESTERN GUATEMALA Taylor Sultan Quedensley1 and Thomas B. Bragg2 1Plant Biology Graduate Program, The University of Texas, 1 University Station A6720, Austin, Texas 78712 2Department of Biology, The University of Nebraska, 6001 Dodge Street, Omaha, Nebraska 68182-0040 Abstract: From 2003 to 2005, 46 genera and 96 species of native Asteraceae were collected on the northwestern slopes of Pico Zunil, a montane cloud forest habitat in southwestern Guatemala. Combining the present survey with past collections, a total of 56 genera and 126 species of Asteraceae have been reported from Pico Zunil, five of which are naturalized Old World species. In the present study, the Heliantheae contains the greatest number of native species (29). The most diverse genus is Ageratina (Eupatorieae, 9 species). Species richness of native Asteraceae measured along an elevational gradient ranged from a low of 16 species at 3400–3542 m to a high of 68 species at 2300–2699 m, where human land use most actively affects cloud forest habitat. Of the plants collected, Ageratina rivalis and Verbesina sousae were new species records for Guatemala. Six more species were new records for the Departmentof Quetzaltenango: Ageratina pichinchensis, A. prunellaefolia, A. saxorum, Koanophyllon coulteri, Stevia triflora, and Telanthophora cobanensis. In addition, 16 of the 96 native species collected are known only from to the western montane departments of Guatemala and the montane regions of southern Chiapas, Mexico. We provide a base of information against which future studies can measure temporal changes in presence of species such as may accompany environmental changes resulting from human activities and/or climate change.
    [Show full text]
  • JOSE L. PANERO JULY 2010 ADDRESS Section Of
    JOSE L. PANERO JULY 2010 ADDRESS Section of Integrative Biology The University of Texas Austin, TX 78712 Ph. (512) 232-1990; Fax: (512) 471-3878 E-mail: [email protected] Citizenship: U.S.A. EDUCATION 1990 Ph.D., Botany, University of Tennessee 1986 M.S., Botany, University of Tennessee 1984 B.A., Biology, University of Miami 1979 High School Diploma, Centro Escolar del Lago, México PROFESSIONAL EXPERIENCE 2002-present Associate Professor of Integrative Biology, and Associate Director of the Plant Resources Center (Herbarium TEX-LL). 1996-2002 Assistant Professor of Botany, and Assistant Director of the Plant Resources Center (Herbarium TEX-LL). 1993-1996 Assistant Professor, and Director, Michigan State University Herbarium. 1991-1993 Research Associate, University of Texas at Austin. 1990-1991 Lecturer, Grinnell College, Grinnell, Iowa. 1988-1990 Graduate Teaching Assistant, University of Tennessee. 1985-1988 Graduate Research Assistant, University of Tennessee. 1984-1985 Graduate Teaching Assistant, University of Tennessee. 1984 Research Assistant, Fairchild Tropical Gardens, Miami, Florida. GRANTS RECEIVED 2004-2007 National Science Foundation, DEB-0344116. Collaborative Research: Systematic Analysis of Evolutionary Radiation of Eupatorieae (Asteraceae). $311, 391. 2004-2006 Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO), CS011. México. Electronic catalogue of Mexican Asteraceae names; final phase. $5, 000. 2002-2004 Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO), V057. México. Electronic Catalogue of Mexican Vascular Plant Specimens Deposited at the University of Texas at Austin Herbaria; fourth phase. $57, 000. 2001-2003 Comisión Nacional para el Conocimiento y Uso de la Biodiversidad (CONABIO), AE024. México. Electronic catalogue of Mexican Asteraceae names; third phase.
    [Show full text]