Xlii Naukowa Konferencja Pszczelarska
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przez Ministerstwo Nauki i Informatyzacji Wydawnictwo materia³ów z Konferencji dofinansowane ISBN 83-88707-57-4 8-9 marca 2005 marca 8-9 AWY £ PU T E D I R S U L U G N A S E N M O I H I M M U R A R R E T E T S I KONFERENCJA PSZCZELARSKA XLII NAUKOW XLII A WO NAUKOWE WO ARZYST TOW ICZE PSZCZELN A TW IC LN ZE ZC PS £ IA DZ OD Redakcja techniczna: Oddzia³ Pszczelnictwa w Pu³awach A TW RS A CI IA KW I A TW IC N W O D SA T TU Y ST IN INDEKS AUTORÓW Aretyns’ka T.B. – 3 Jagie³³o Ryszard – 146 Arkhypov A.O. – 3, 33 Jasiñski Zygmunt – 28, 30 Bakier S³awomir – 136, 138, 140 Jendreják Rudolf – 76 Balzekas Jonas – 62 Jojczyk Agata – 28 B¹k Beata – 28, 60 Kaba³a-Dzik Agata – 160 Bieñkowska Ma³gorzata – 21, 25, 65 Kamler František – 38 Blaþytë-Èereðkienë Laima – 16 Kania Artur – 30 Bogdanov G. – 55, 142 Katysheva Olga – 131 Bondarchuk L.I. – 55 Kazimierczak Jerzy –69 Borisov Vadim – 131 Kêdzia Bogdan – 144, 156 Borsuk Grzegorz – 34, 146 Klepacz-Baniak Joanna – 99 Bo¿ek Ma³gorzata – 91, 92 Kokta O.D. – 85 Brajczewska Beata – 44 Kolbina Lidia – 39, 100 Bratkowski Janusz – 22, 28, 36, 56 Ko³towski Zbigniew – 102, 103 Buczek Krzsztof – 72, 73 Komissar Alexander – 14, 40 Èeksterytë Violeta – 37 Konarska Agata – 95, 105, 122 Chmielewski Wit – 132 Kopernický Ján – 42 Chorbiñski Pawe³ – 4, 23, 68, 89 Korpysa Gra¿yna – 43 Chuda-Mickiewicz Bo¿ena – 28, 69 Kotsjumbas I.J. – 55 Chwil Miros³awa – 93, 95 Kravetsky L.J. – 33, 84 Ciowch Agnieszka – 146 Krieg Pavel – 133 Czekoñska Krystyna – 71, 99 Krivtsova L.S. – 26 Denisow B. – 96, 97 Krzy¿añska Katarzyna – 78 Dmitruk Marta – 121 Kwiatkowski Stanis³aw – 106, 108, 121 Dronik G. – 142 Kyuryishkin Mihckail – 100 Gerula Dariusz – 21, 65 Labaèiauskas Jonas – 37 Giejdasz Karol – 134 Levchenko Ivan – 6, 8 Gliñski Zdzis³aw – 72, 73 Lipiec Antoni – 146 Gontarz Aldona – 25 Lipiñski Zbigniew – 6 Grankin N.N. – 26 Loc Krzysztof – 25 Grzebalska Agnieszka – 153 Lokutova O. – 110, 142 Hajnos Micha³ – 106 Londzin Wies³aw – 79, 80 Hoffman Marian – 50, 52 Luft-Deptu³a Dorota – 72, 73 Ho³derna-Kêdzia El¿bieta – 144 Lutsenko Yriy – 8 Hoñko Stanis³aw – 75 Madras Beata – 28 Huras Bogumi³a – 69 Marzec Janina – 57 Irzyk Maria – 79, 80 Masierowska Marzena – 105 Ivchenko V. – 6 Medvedeva Anna – 131 Mikhailov A. – 142 Sokó³ Rajmund – 87 Mitrowska Kamila – 148 Stawiarz Ernest – 150 Motoshkova Natalia – 100 Stojko Artur – 158, 160 Musyenko O.V. – 86 Stojko Jerzy – 160 Najda Agnieszka – 106, 108 Strza³kowska Monika – 116, 119 Nepeivoda Sofia – 39, 100 Sulborska Aneta – 118 Neumann Peter – 22 Szczêsna Teresa – 149, 152 Niedzielska Jolanta – 148 Szklanowska Kazimiera –119 Olszewski Krzysztof – 10 Szkoda Józef – 153 Paleolog Jerzy – 10, 34 Szymula Jerzy – 21, 31, 65 Pa³ach Ryszard S. – 43 Teper Dariusz – 110, 154 Panasiuk Beata – 21, 65 Tlusta Yulia P. – 3 Pêkala Leszek – 138 Tlusty Anatoliy – 16 Piech Katarzyna – 134 Tofilski Adam – 32 Piletskaya Irina – 81 Tomaszewska Barbara – 4, 23, 68, 89 Piotrowska Krystyna – 111, 113 Trikoz V.O. – 3 Pliszczyñski Mariusz – 72, 73 Tsigankov N. – 142 Pohorecka Krystyna – 65, 83 Vorobiy O.A. – 84 Polaczek Benedikt – 134 Weryszko-Chmielewska El¿bieta – 95,105,121, 122 Polishuk V. – 110 Wieloch Grzegorz – 50, 52 Popoviè Ivan – 115 Wieniarska Justyna – 92 Postoenko V.O. – 84, 85 Wiêckowska Joanna – 12 Posyniak Andrzej – 148 Wilde Jerzy – 17, 22, 28, 36, 56, 60 Prabucki Jaros³aw – 28, 69 Wilde Maria – 17 Raèys Jurgis – 37 Wilkaniec Zdzis³aw – 134 Rogoziñski Tomasz – 50, 52 Witkiewicz Wies³aw – 46 Roman Adam – 12, 44 W³odarczyk Marek – 4, 23, 68, 89 Romaniuk Konstanty – 46 Wolski Tadeusz – 106, 108, 121 Rudenko E.V. – 86 Woyke Jerzy – 17, 28 Rybak-Chmielewska Helena – 149, 152 Wójcik Joanna – 144 Rzepecka Anna – 160 Wróblewska Anna – 123 Samborski Jerzy – 28, 69 Wrzesieñ M. – 96 Schricker Burkhard – 134 Yefimenko Tatiana M. – 3, 19 Semkiw Piotr – 48 Zorina Marina – 100 Shumakova Irina – 14 Zvinchuk I.O. – 85 Sieben Stefan – 134 ¯mudzki Jan – 148, 153 Siuda Maciej – 28, 36, 56, 60 ¯uraw Beata – 125, 126, 127, 128 Skirkevièius Algirdas – 16 Skowronek Wojciech – 48 Skubida Piotr – 48 BEE BIOLOGY - BIOLOGIA THE EFFECT OF EXTRACTS FROM ANTHERAEAE PERNYI PUPPAE ON THE HONEY BEE, Apis mellifera A.O. Arkhipov1 , Tatiana M. Yefimenko1 , Yulia P. Tlusta1 , T.B. Aretyns’ka2 , V.O. Trikoz2 1 Petro Prokopovych Apiculture Institute (Kyiv, Ukraine). 2 National University of Agriculture (Kyiv, Ukraine). The authors investigated the effect of the Antheraeae pernyi puppae extract on the honey bee, Apis mellifera, the extract having been proposed as a drug (Trokoz et al. Patent of Ukraine ¹ 16965; 1997) possessing rather marked adaptogene properties for many animals (Trokoz et al. 2003). In our laboratory we studied optimal concentrations to be fed to healthy honey bees and the extract effect on these insects artificially infected by a microsporidian, Nosema apis. We have also investigated the effect of optimal extract concentration in natural conditions, these experiments having been carried out with bee colonies inhabited in hives with natural levels of invasive and infective diseases. In laboratory conditions the extract was fed together with sugar syrup (50%), in field experiments with the candy. Three drug concentrations were studied – 0.5, 0.1, and 0 0.01%. In laboratory experiments with bees preliminary infected by the N. apis, the insects were fed by this parasite spores (103 spores/per bee). Control bees were without infection and without extract introduction. Our laboratory experiments were carried out with flying bees taken preliminary to cages and maintained in a thermostat, the temperature and humidity conditions being the optimal. The insects were observed on the every second day. The death factors were determined by microscopic analysis of dead insects. In field conditions the bee colonies were found to be moderately or heavily infected by N. apis (Grobov 1987). Colonies chosen for experiments were genetically similar containing bees with the similar reproductive potencies. Both in experimental and control colonies, 50 insects were taken for microscopic analysis to determine the dynamics of nosematosis level decrease according to the drop of N.apis spore concentration in bees investigated. Our laboratory experiments show the optimal Antheraeae pernyi puppae extract concentration studied is 0.1%. The bees fed by such extract may survive by 8.31 ± 1.51 days (i.e. by 35%) longer comparing to control ones. The use of such extract (0.1%) for artificially diseased bees and healthy ones accelerates the dying out of N.apis-infected insects by 3.5 ± 2.5 days (i.e. by 8.2 %) (P<0.95) comparing to non-treated diseased bees; at the same time the drug introduction to healthy insects makes their life longer by 10.6 ± 2.5 days (P>0.999) (i.e. by 35.6%). According to microscopy data, the main bee death factor is their heavy invasion by the parasite. In natural condition, the use of candy containing the puppae extract (0.1 %) increases significantly the brood development, the number of bees obtained becoming higher by 11,127 ± 9,625 insects (i.e. 35.5%) comparing to the control. Different nosematosis Materia³y z Naukowej Konferencji Pszczelarskiej, Pu³awy 2005 3 effects on bees led to non-significant difference between experimental and control bee groups. According to the microscopy data, the drug introduction led to decreased bee infection by the N. apis in experimental bee groups comparing to control ones; therefore, we observed the stimulating drug effect both on bees and their parasite. This fact confirms the Steche idea (1977) about stimulating bee feeding which increases both brood and nosematosis acceleration. Our data suggest a favorable effect of Antheraeae pernyi puppae extract on healthy bees. Our preparation (0.1 %) might be used to make the bee life longer and to increase their reproductive potencies (Yefimenko et al. 2004; Patent of Ukraine ¹ 68304 À). However, taking the negative drug effect on infected bees, we think this stimulator to be used only in bee colonies free from clinical nosematosis. 1. Patent of Ukraine ¹ 16965. An approach to obtain a therapeutic extract/V.Î. Trokoz, T.D. Lotosh, A.B. Abramova et al. – Received 03.10.89. – Published 29.06.97. – Bull. N 7 (in Ukrainian). 2. Patent of Ukraine ¹ 68304 À. An approach to increase the life potencies of the honey bee/T.M. Yefimenko, A.O. Arkhipov, Yu.P. Omelchenko et al. – Received 03.12.2003. – Published 15.07.2004. – Bull.N7. 3. Steche W. Unsolved problems of the Nosema apis Z. biology. In the: Biological Problems of Nosematosis. Apimondia Symposium on Honey Bee Biology & Pathology (Merelbecke, Belgium, 14-16 July, 1976. - Bucharest. - 1977. - P.23 - 36. 4. Trokoz V.O., Aretynska T.B., Yefimenko T.M., Trokoz N.V. The problem of Antheraeae pernyi development and use of different trees leaves for their feeding / Lisivnytstvo (Forest Science). - Vol 4. – NN 1-2. – 2003. – p. 81-84 (in Ukrainian). PRÓBY OKREŒLENIA ¯YWOTNOŒCI PLEMNIKÓW W ZBIORNICZKACH NASIENNYCH M£ODYCH MATEK PSZCZELICH Pawe³ Chorbiñski, Marek W³odarczyk, Barbara Tomaszewska Katedra Epizootiologii i Administracji Weterynaryjnej, Wydzia³ Medycyny Weterynaryjnej AR we Wroc³awiu Materia³ badawczy stanowi³o 65 prób nasienia pobranego ze zbiorników nasien- nych m³odych inseminowanych matek pszczelich. Przyjêto za³o¿enie, ¿e w 1 zbior- niczku nasienia u matki zawarte jest od 4,5 do 5,7 × 106 plemników. Matki pszczele uœmiercano przez dekapitacje. Przy u¿yciu lupy binokularnej wyp- reparowywano z nich zbiorniki nasienia. Zawartoœæ pojedynczego zbiornika nasienia zawieszano w buforze tak, aby otrzymaæ koncentracjê ok. 1 × 106 plemników w 1000 µl buforu.