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Development and Maintenance of Epidermal Stem Cells in Skin Adnexa
International Journal of Molecular Sciences Review Development and Maintenance of Epidermal Stem Cells in Skin Adnexa Jaroslav Mokry * and Rishikaysh Pisal Medical Faculty, Charles University, 500 03 Hradec Kralove, Czech Republic; [email protected] * Correspondence: [email protected] Received: 30 October 2020; Accepted: 18 December 2020; Published: 20 December 2020 Abstract: The skin surface is modified by numerous appendages. These structures arise from epithelial stem cells (SCs) through the induction of epidermal placodes as a result of local signalling interplay with mesenchymal cells based on the Wnt–(Dkk4)–Eda–Shh cascade. Slight modifications of the cascade, with the participation of antagonistic signalling, decide whether multipotent epidermal SCs develop in interfollicular epidermis, scales, hair/feather follicles, nails or skin glands. This review describes the roles of epidermal SCs in the development of skin adnexa and interfollicular epidermis, as well as their maintenance. Each skin structure arises from distinct pools of epidermal SCs that are harboured in specific but different niches that control SC behaviour. Such relationships explain differences in marker and gene expression patterns between particular SC subsets. The activity of well-compartmentalized epidermal SCs is orchestrated with that of other skin cells not only along the hair cycle but also in the course of skin regeneration following injury. This review highlights several membrane markers, cytoplasmic proteins and transcription factors associated with epidermal SCs. Keywords: stem cell; epidermal placode; skin adnexa; signalling; hair pigmentation; markers; keratins 1. Epidermal Stem Cells as Units of Development 1.1. Development of the Epidermis and Placode Formation The embryonic skin at very early stages of development is covered by a surface ectoderm that is a precursor to the epidermis and its multiple derivatives. -
Abnormal Hair Follicle Development and Altered Cell Fate of Follicular
ERRATUM Development 137, 1775 (2010) doi:10.1242/dev.053116 Abnormal hair follicle development and altered cell fate of follicular keratinocytes in transgenic mice expressing Np63 Rose-Anne Romano, Kirsten Smalley, Song Liu and Satrajit Sinha There was a technical problem with the ePress version of Development 137, 1431-1439 published on 24 March 2010. A number of Greek symbols were missing from the text and figure headings in the Results section. A replacement ePress article was published on 31 March 2010. The online issue and print copy are correct. We apologise to authors and readers for this error. DEVELOPMENT AND STEM CELLS RESEARCH ARTICLE 1431 Development 137, 1431-1439 (2010) doi:10.1242/dev.045427 © 2010. Published by The Company of Biologists Ltd Abnormal hair follicle development and altered cell fate of follicular keratinocytes in transgenic mice expressing DNp63a Rose-Anne Romano1, Kirsten Smalley1, Song Liu2,3 and Satrajit Sinha1,* SUMMARY The transcription factor p63 plays an essential role in epidermal morphogenesis. Animals lacking p63 fail to form many ectodermal organs, including the skin and hair follicles. Although the indispensable role of p63 in stratified epithelial skin development is well established, relatively little is known about this transcriptional regulator in directing hair follicle morphogenesis. Here, using specific antibodies, we have established the expression pattern of DNp63 in hair follicle development and cycling. DNp63 is expressed in the developing hair placode, whereas in mature hair its expression is restricted to the outer root sheath (ORS), matrix cells and to the stem cells of the hair follicle bulge. To investigate the role of DNp63 in hair follicle morphogenesis and cycling, we have utilized a Tet-inducible mouse model system with targeted expression of this isoform to the ORS of the hair follicle. -
Anatomy and Physiology of Hair
Chapter 2 Provisional chapter Anatomy and Physiology of Hair Anatomy and Physiology of Hair Bilgen Erdoğan ğ AdditionalBilgen Erdo informationan is available at the end of the chapter Additional information is available at the end of the chapter http://dx.doi.org/10.5772/67269 Abstract Hair is one of the characteristic features of mammals and has various functions such as protection against external factors; producing sebum, apocrine sweat and pheromones; impact on social and sexual interactions; thermoregulation and being a resource for stem cells. Hair is a derivative of the epidermis and consists of two distinct parts: the follicle and the hair shaft. The follicle is the essential unit for the generation of hair. The hair shaft consists of a cortex and cuticle cells, and a medulla for some types of hairs. Hair follicle has a continuous growth and rest sequence named hair cycle. The duration of growth and rest cycles is coordinated by many endocrine, vascular and neural stimuli and depends not only on localization of the hair but also on various factors, like age and nutritional habits. Distinctive anatomy and physiology of hair follicle are presented in this chapter. Extensive knowledge on anatomical and physiological aspects of hair can contribute to understand and heal different hair disorders. Keywords: hair, follicle, anatomy, physiology, shaft 1. Introduction The hair follicle is one of the characteristic features of mammals serves as a unique miniorgan (Figure 1). In humans, hair has various functions such as protection against external factors, sebum, apocrine sweat and pheromones production and thermoregulation. The hair also plays important roles for the individual’s social and sexual interaction [1, 2]. -
Diapositiva 1
Ingegneria delle tecnologie per la salute Fondamenti di anatomia e istologia Apparato tegumentario aa. 2017-18 INTEGUMENTARY SYSTEM integumentary system = refers to skin and its accessory structures responsible for much more than simply human outward appearance: about 16% of body weight, covering an area of 1.5 to 2 m2 (= largest organ system in human body). • skin protects inner organs INTEGUMENTARY SYSTEM • skin = even not typical, but an organ, made of tissues that work together as a single structure to perform unique and critical functions • integumentary system = skin + its accessory structures, providing body with overall protection. • made of multiple layers of cells and tissues, which are held to underlying structures by connective tissue: deeper layer of skin is well vascularized (has numerous blood vessels) and also has numerous sensory, and autonomic and sympathetic nerve fibers ensuring communication to and from brain. INTEGUMENTARY SYSTEM Overview • Largest organ (15% of body weight) • Epidermis – keratinized stratified squamous epithelium • Dermis – connective tissue layer • Hypodermis • Thickness variable, normally 1-2 mm – dermis may thicken, up to 6 mm – stratum corneum layer increased • calluses on hands and feet Structure of the Skin 2 layers: epidermis + dermis SKIN: histology SKIN: histology SKIN: histology Cells of the Epidermis • Stem cells – undifferentiated cells in deepest layers • Keratinocytes – most of the skin cells • Melanocytes – synthesize pigment that shield UV • Tactile (merkel) cells – receptor cells associated with nerve fibers • Dendritic (langerhans) cells – macrophages guard against pathogens Cell and Layers of the Epidermis Epidermis: histology = composed of keratinized, stratified squamous epithelium, made of 4 or 5 layers of epithelial cells, depending on its location in body. -
Skates and Rays Diversity, Exploration and Conservation – Case-Study of the Thornback Ray, Raja Clavata
UNIVERSIDADE DE LISBOA FACULDADE DE CIÊNCIAS DEPARTAMENTO DE BIOLOGIA ANIMAL SKATES AND RAYS DIVERSITY, EXPLORATION AND CONSERVATION – CASE-STUDY OF THE THORNBACK RAY, RAJA CLAVATA Bárbara Marques Serra Pereira Doutoramento em Ciências do Mar 2010 UNIVERSIDADE DE LISBOA FACULDADE DE CIÊNCIAS DEPARTAMENTO DE BIOLOGIA ANIMAL SKATES AND RAYS DIVERSITY, EXPLORATION AND CONSERVATION – CASE-STUDY OF THE THORNBACK RAY, RAJA CLAVATA Bárbara Marques Serra Pereira Tese orientada por Professor Auxiliar com Agregação Leonel Serrano Gordo e Investigadora Auxiliar Ivone Figueiredo Doutoramento em Ciências do Mar 2010 The research reported in this thesis was carried out at the Instituto de Investigação das Pescas e do Mar (IPIMAR - INRB), Unidade de Recursos Marinhos e Sustentabilidade. This research was funded by Fundação para a Ciência e a Tecnologia (FCT) through a PhD grant (SFRH/BD/23777/2005) and the research project EU Data Collection/DCR (PNAB). Skates and rays diversity, exploration and conservation | Table of Contents Table of Contents List of Figures ............................................................................................................................. i List of Tables ............................................................................................................................. v List of Abbreviations ............................................................................................................. viii Agradecimentos ........................................................................................................................ -
Vocabulario De Morfoloxía, Anatomía E Citoloxía Veterinaria
Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) Servizo de Normalización Lingüística Universidade de Santiago de Compostela COLECCIÓN VOCABULARIOS TEMÁTICOS N.º 4 SERVIZO DE NORMALIZACIÓN LINGÜÍSTICA Vocabulario de Morfoloxía, anatomía e citoloxía veterinaria (galego-español-inglés) 2008 UNIVERSIDADE DE SANTIAGO DE COMPOSTELA VOCABULARIO de morfoloxía, anatomía e citoloxía veterinaria : (galego-español- inglés) / coordinador Xusto A. Rodríguez Río, Servizo de Normalización Lingüística ; autores Matilde Lombardero Fernández ... [et al.]. – Santiago de Compostela : Universidade de Santiago de Compostela, Servizo de Publicacións e Intercambio Científico, 2008. – 369 p. ; 21 cm. – (Vocabularios temáticos ; 4). - D.L. C 2458-2008. – ISBN 978-84-9887-018-3 1.Medicina �������������������������������������������������������������������������veterinaria-Diccionarios�������������������������������������������������. 2.Galego (Lingua)-Glosarios, vocabularios, etc. políglotas. I.Lombardero Fernández, Matilde. II.Rodríguez Rio, Xusto A. coord. III. Universidade de Santiago de Compostela. Servizo de Normalización Lingüística, coord. IV.Universidade de Santiago de Compostela. Servizo de Publicacións e Intercambio Científico, ed. V.Serie. 591.4(038)=699=60=20 Coordinador Xusto A. Rodríguez Río (Área de Terminoloxía. Servizo de Normalización Lingüística. Universidade de Santiago de Compostela) Autoras/res Matilde Lombardero Fernández (doutora en Veterinaria e profesora do Departamento de Anatomía e Produción Animal. -
Corrective Gene Transfer of Keratinocytes from Patients with Junctional Epidermolysis Bullosa Restores Assembly of Hemidesmosomes in Reconstructed Epithelia
Gene Therapy (1998) 5, 1322–1332 1998 Stockton Press All rights reserved 0969-7128/98 $12.00 http://www.stockton-press.co.uk/gt Corrective gene transfer of keratinocytes from patients with junctional epidermolysis bullosa restores assembly of hemidesmosomes in reconstructed epithelia J Vailly1, L Gagnoux-Palacios1, E Dell’Ambra2, C Rome´ro1, M Pinola3, G Zambruno3, M De Luca2,3 J-P Ortonne1,4 and G Meneguzzi1 1U385 INSERM, Faculte´ de Me´decine, Nice; 4Service de Dermatologie, Hoˆpital L’Archet, Nice, France; Laboratories of 2Tissue Engineering and 3Molecular and Cell Biology, Istituto Dermopatico dell’Immacolata, Rome, Italy Herlitz junctional epidermolysis bullosa (H-JEB) provides deposited into the extracellular matrix. Re-expression of a promising model for somatic gene therapy of heritable laminin-5 induced cell spreading, nucleation of hemides- mechano-bullous disorders. This genodermatosis is mosomal-like structures and enhanced adhesion to the cul- caused by the lack of laminin-5 that results in absence of ture substrate. Organotypic cultures performed with the hemidesmosomes (HD) and defective adhesion of squam- transduced keratinocytes, reconstituted epidermis closely ous epithelia. To establish whether re-expression of lami- adhering to the mesenchyme and presenting mature hemi- nin-5 can restore assembly of the dermal-epidermal attach- desmosomes, bridging the cytoplasmic intermediate fila- ment structures lacking in the H-JEB skin, we corrected the ments of the basal cells to the anchoring filaments of the genetic mutation hindering expression of the 3 chain of basement membrane. Our results provide the first evi- laminin-5 in human H-JEB keratinocytes by transfer of a dence of phenotypic reversion of JEB keratinocytes by laminin 3 transgene. -
The Distribution of Sweat Glands Over the Human Body Has So Far Been In
NOTES ON THE VERTICAL DISTRIBUTION OF THE HUMAN SWEAT GLANDS SHUNZO TAKAGI AND KO TOBARU* Institute of Physiology, School of Medicine, University of Nagoya•õ The distribution of sweat glands over the human body has so far been in- vestigated in the dimension of area, and we have no general idea how deep they are distributed in the skin. In 1943, Kuno and his collaborators (3) expressed the opinion that chloride would be accumulated in the skin during the activity of sweat glands. The truth of this assumption has been confirmed with more certainty by Yoshimura and Chihaya (unpublished), who measured the chloride content in the skin tissue by means of Ag-AgCl electrodes. The chloride may presumably be accumulated in the immediate neighbourhood of the glomeruli of sweat glands, or more diffusely in the layers of skin tissues where the glomeruli are situated. For consideration of the amount of the accumulated chloride, the total volume of these skin layers, which can be estimated by the vertical distribution of the sweat-gland glomeruli, seems to be useful. The fol- lowing investigation was therefore performed. MATERIALS AND METHOD Skin samples of 33 regions of the body, as specified in table 1, were taken from the corpse of a Japanese male of 30 years old, who died an accidental death. The samples were fixed in 10 per cent formalin, embedded in celloidin and cut into sections 15 micra thick. The sections were stained with Delafield's hematoxylin and eosin. Observations of sweat glands were made with 2-3 pieces of the skin about 100 sq. -
Long-Lasting Muscle Thinning Induced by Infrared Irradiation Specialized with Wavelengths and Contact Cooling: a Preliminary Report
Long-Lasting Muscle Thinning Induced by Infrared Irradiation Specialized With Wavelengths and Contact Cooling: A Preliminary Report Yohei Tanaka, MD, Kiyoshi Matsuo, MD, PhD, and Shunsuke Yuzuriha, MD, PhD Department of Plastic and Reconstructive Surgery, Shinshu University School of Medicine, Matsumoto, Nagano 390-8621, Japan Correspondence: [email protected] Published May 28, 2010 Objective: Infrared (IR) irradiation specialized with wavelengths and contact cooling increases the amount of water in the dermis to protect the subcutaneous tissues against IR damage; thus, it is applied to smooth forehead wrinkles. However, this treatment consistently induces brow ptosis. Therefore, we investigated whether IR irradiation induces muscle thinning. Methods: Rat central back tissues were irradiated with the specialized IR device. Histological evaluation was performed on sagittal slices that included skin, panniculus carnosus, and deep muscles. Results: Significant reductions in panniculus carnosus thickness were observed between controls and irradiated tissues at postirradiation day 30 (P30), P60, P90, and P180; however, no reduction was observed in nonirradiated controls from days 0 to 180. No significant changes were observed in the trunk muscle over time. From day 0, dermal thickness was significantly reduced at P90 and P180; however, no difference was observed between P180 and nonirradiated controls at day 180. DNA degradation consistent with apoptosis was detected in the panniculus carnosus at P7 and P30. Conclusions: We found that IR irradiation induced long-lasting superficial muscle thinning, probably by a kind of apoptosis. The panniculus carnosus is equivalent to the superficial facial muscles of humans; thus, the changes observed here reflected those in the frontalis muscle that resulted in brow ptosis. -
Enabling Sweat-Based Biosensors: Solving the Problem of Low
Enabling sweat-based biosensors: Solving the problem of low biomarker concentration in sweat A dissertation submitted to the Graduate School of the University of Cincinnati in partial fulfillment of the requirements for the degree of Doctor of Philosophy in the Department of Biomedical Engineering of the College of Engineering & Applied Science by Andrew J. Jajack B.S., Biology, Wittenberg University, 2014 Committee Chairs: Jason C. Heikenfeld, Ph.D. and Chia-Ying Lin, Ph.D. Abstract Non-invasive, sweat biosensing will enable the development of an entirely new class of wearable devices capable of assessing health on a minute-to-minute basis. Every aspect of healthcare stands to benefit: prevention (activity tracking, stress-level monitoring, over-exertion alerting, dehydration warning), diagnosis (early-detection, new diagnostic techniques), and management (glucose tracking, drug-dose monitoring). Currently, blood is the gold standard for measuring the level of most biomarkers in the body. Unlike blood, sweat can be measured outside of the body with little inconvenience. While some biomarkers are produced in the sweat gland itself, most are produced elsewhere and must diffuse into sweat. These biomarkers come directly from blood or interstitial fluid which surrounds the sweat gland. However, a two-cell thick epithelium acts as barrier and dilutes most biomarkers in sweat. As a result, many biomarkers that would be useful to monitor are diluted in sweat to concentrations below what can be detected by current biosensors. This is a core challenge that must be overcome before the advantages of sweat biosensing can be fully realized. The objective of this dissertation is to develop methods of concentrating biomarkers in sweat to bring them into range of available biosensors. -
An Analysis of Benign Human Prostate Offers Insights Into the Mechanism
www.nature.com/scientificreports OPEN An analysis of benign human prostate ofers insights into the mechanism of apocrine secretion Received: 12 March 2018 Accepted: 22 February 2019 and the origin of prostasomes Published: xx xx xxxx Nigel J. Fullwood 1, Alan J. Lawlor2, Pierre L. Martin-Hirsch3, Shyam S. Matanhelia3 & Francis L. Martin 4 The structure and function of normal human prostate is still not fully understood. Herein, we concentrate on the diferent cell types present in normal prostate, describing some previously unreported types and provide evidence that prostasomes are primarily produced by apocrine secretion. Patients (n = 10) undergoing TURP were prospectively consented based on their having a low risk of harbouring CaP. Scanning electron microscopy and transmission electron microscopy was used to characterise cell types and modes of secretion. Zinc levels were determined using Inductively Coupled Plasma Mass Spectrometry. Although merocrine secretory cells were noted, the majority of secretory cells appear to be apocrine; for the frst time, we clearly show high-resolution images of the stages of aposome secretion in human prostate. We also report a previously undescribed type of epithelial cell and the frst ultrastructural image of wrapping cells in human prostate stroma. The zinc levels in the tissues examined were uniformly high and X-ray microanalysis detected zinc in merocrine cells but not in prostasomes. We conclude that a signifcant proportion of prostasomes, possibly the majority, are generated via apocrine secretion. This fnding provides an explanation as to why so many large proteins, without a signal peptide sequence, are present in the prostatic fuid. Tere are many complications associated with the prostate from middle age onwards, including benign prostatic hyperplasia (BPH) and prostate cancer (PCa). -
Periodic Acid-Schiff Positive Material Accumulating Within the Lumen of Eccrine Sweat Glands*
View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector PERIODIC ACID-SCHIFF POSITIVE MATERIAL ACCUMULATING WITHIN THE LUMEN OF ECCRINE SWEAT GLANDS* GEORGE W. HAMBRICK, JR., M.D. The purpose of this paper is to record findings with regard to diastase-resistant, periodic acid-Schiff positive material in the lumen of eccrine ducts and glands of two individuals. This material has two possible sources in the normal eccrine sweat gland, namely, the cuticle lining of the eccrine duct and the cells of the secretory tubule. Holyoke and Lobits (3) studying 35 normal skin biopsies re- B pp HI D FIG. 1. A. Vertical section through skin of the control area showing dilatation of an eccrine sweat duct with eosinophilic material in the lumen. H. and E., X178. B. Same find- ings as in A above from an area treated daily with 3 per cent hexachloronaphthalene in acetone for one week, Xl7S. C. Section through dermal parts of eccrine duct containing a cast. H. and E., X355. D. Section through dermal eccrine duct containing periodic acid- Schiff positive material in the lumen, X660. *Fromthe Department of Dermatology (Donald M. Pillsbury, M.D., Director), School of Medicine, University of Pennsylvania, Philadelphia 4, Pennsylvania. This study was supported by U. S. Army grant DA-49-007-MD-154. Received for publication March 29, 1957. 213 214 THF JOURNAL OF INVESTIGATIVE DERMATOLOGY ported the presence of material in the lumen of the eccrine duct and gland; they classified the material as amorphous, cast, cellular or bacterial.