(12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2014/132141 A2 4 September 2014 (04.09.2014) P O P C T

(51) International Patent Classification: Not classified KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (21) International Application Number: OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, PCT/IB20 14/00 1106 SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, (22) International Filing Date: TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, 3 1 January 2014 (3 1.01 .2014) ZW. (25) Filing Language: English (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, (26) Publication Language: English GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, (30) Priority Data: UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, 61/759,099 31 January 2013 (3 1.01.2013) US TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, (71) Applicants: BASF SE [DE/DE]; Carl-Bosch-Strasse 38, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, 67056 Ludwigshafen am Rhein (DE). UNIVERSITY OF TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, GUELPH [CA/CA]; Business Development Office, 130 KM, ML, MR, NE, SN, TD, TG). Research Lane, Unit 4, Guelph, Ontario, NIG 5G3 (CA). Declarations under Rule 4.17 : (72) Inventors: HALL, J., Christopher; 31 Lambert Crescent, — as to applicant's entitlement to applyfor and be granted a Guelph, ON NIG 2R4 (CA). JUGULAM, Mithila; 2301 patent (Rule 4.1 7(H)) Hillview Drive, Manhattan, KS 66502 (US). MANKIN, Scots, Llewellyn; 4800 Deerwood Drive, Raleigh, NC — as to the applicant's entitlement to claim the priority of the 27612 (US). WESTON, Brigette, J.; Roemerweg 103a, earlier application (Rule 4.1 7(in)) 67434 Neustadt (DE). Published: (74) Agents: SIEGERT, G. Dr. et al; Hoffmann . Eitle, Patent — without international search report and to be republished und Rechtsanwalte, Arabellastrasse 4, 81925 Munchen upon receipt of that report (Rule 48.2(g)) (DE). — with (an) indication^) in relation to deposited biological (81) Designated States (unless otherwise indicated, for every material furnished under Rule 13bis separately from the kind of national protection available): AE, AG, AL, AM, description (Rules 13bis.4(d)(i) and 48.2(a)(viii)) AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, — with sequence listingpart of description (Rule 5.2(a)) DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR,

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(54) Title: AUXINIC -TOLERANT PLANTS (57) Abstract: Described herein are auxmic herbicide-tolerant plants, auximc herbicide-tolerance characteristics, and nucleic acids and polypeptides conferring said auximc herbicide-tolerance characteristics. Also described are methods for controlling the growth of weeds by applying an auximc herbicide to which the auximc herbicide -tolerant plants described herein are tolerant. AUXINIC HERBICIDE-TOLERANT PLANTS

RELATED APPLICATIONS This application claims priority under 35 U.S.C. § 119 to U.S. Provisional Patent Application No. U.S. 61/759,099, that was filed on January 31, 2013, and is incorporated by reference herein in its entirety.

TECHNICAL FIELD This application relates to the field of agriculture, particularly to auxinic herbicide- tolerant plants.

BACKGROUND Auxinic herbicide tolerance has been reported as occurring in weeds, such as Kochia and Charlock, but not in Brassica crop plants, such as domesticated B. napus, B. rapa, or B.juncea. See, e.g., Nandula and Mathey (2002); Jugulam et al. (2005). Attempts have been made to provide an auxinic herbicide tolerance trait for use in Brassica crop plants, but to date these have not been demonstrated to provide a commercial level of herbicide tolerance in such plants; moreover, these attempts have involved transgenic insertion of exogenous auxinic herbicide tolerance trait genes. See, e.g., U.S. Patent Nos. 8,071,847 and 8,603,755. The herbicide tolerance of the mustard weed reported by Jugulam et al. (2005) has, despite intense efforts, eluded discovery and thus has not been successfully transferred into crop plants. See, e.g., Jugulam et al. (2005); Jasienuik et al. (1995). It would be useful to obtain crop plants that possess auxinic herbicide tolerance. Yet, lacking definitive information as to which gene, gene product, or mechanism of action may be involved in such tolerance in the above-described weeds, it has not been possible to leverage this discovery in mustard weed to a useful application in crop plants. Thus, there remains a need for Brassica crop plants exhibiting a commercial level of tolerance to auxinic herbicide(s), and for such auxinic herbicide tolerant Brassica crop plants that are non-transgenic, as well as for plant parts, seeds, and products thereof. BRIEF SUMMARY One embodiment described herein is an auxinic herbicide tolerant crop plant having a commercial level of tolerance to an auxinic herbicide. Another aspect described herein is a non- transgenic auxinic herbicide tolerant crop plant having a commercial level of tolerance to an auxinic herbicide. Another embodiment described herein is an auxinic herbicide tolerant plant or plant part thereof having the auxinic herbicide-tolerance characteristic of any of Sinapis arvensis lines DT- 0 1 SA2-R or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent Deposit Designation Numbers PTA-1 1213 and PTA-1 1214, respectively, with the proviso that the plant is a monocot or dicot species other than Sinapis arvensis. In one aspect, the auxinic herbicide tolerance trait is referred to as "DART." Lines DT-01 SA2-R and DT-01 BC8SA2-R also are known as "SaParR" and "SaBC8R," respectively. Another embodiment described herein is a nucleic acid comprising: (a) a chimeric polynucleotide comprising both Sinapis arvensis nucleotide sequence and Brassica nucleotide sequence, wherein said polynucleotide encodes the DART trait of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT- 0 1 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent Deposit Designation Numbers PTA-

120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA-1 1213, and PTA-1 1214, respectively; or (b) a mutagenized or recombinant polynucleotide encoding the DART trait of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with

ATCC under Patent Deposit Designation Numbers PTA-120132, PTA-1 121 1, PTA-12050, PTA- 11212, PTA-1 1213, and PTA-1 1214, respectively. In one aspect, the DART trait is encoded by the Sinapis arvensis polynucleotide. In another aspect, the nucleic acid encodes a functional DART trait, said nucleic acid comprising (a) a nucleotide sequences according to any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80; or (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence according to any one of SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 81, respectively; or a mature form thereof. In some aspects, a nucleotide sequence encoding the amino acid sequence is at least 90% homologous to any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, or a synonymous codon substituted variant thereof. In other aspects, the encoded amino acid sequence is 70%> homologous to any one of SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 81, or a mature form thereof. In another aspect, the polynucleotide is isolated. In another aspect, the nucleic acid is operably linked to a promoter operable in plant cells capable of expressing the polypeptide encoded by the nucleic acid, the expression of the polypeptide conferring to the plant or cell tolerance to an auxinic herbicide. Another embodiment described herein is an auxinic herbicide tolerant plant or plant part thereof having the auxinic herbicide-tolerance characteristic of any of Brassica napus lines DT- 0 1 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, or DT-01 BC5Bn#13-l-18, a representative sample of seed of each line having been deposited with American Type Culture

Collection (ATCC) under Patent Deposit Designation Numbers PTA-120132, PTA-1 121 1, PTA- 12050, and PTA-1 1212, respectively, with the proviso that the plant is a monocot or dicot species other than Sinapis arvensis; and wherein the auxinic herbicide tolerance is greater than that exhibited by a wild type variety of said plant lacking said auxinic herbicide tolerance. Introgressed Brassica napus lines containing an auxinic herbicide tolerant trait include DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, and DT-01 BC5Bn#13-l-18. In one aspect, the auxinic herbicide tolerance trait present in any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, is referred to as "DART." In some aspects, the auxinic herbicide tolerant plant is a Brassica juncea, B. napus, or B. rapa plant. In other aspects, the auxinic herbicide tolerant plant is a canola plant. Another embodiment is a descendant of the auxinic herbicide tolerant plant wherein the descendant has the auxinic herbicide-tolerance characteristic. In one aspect, the auxinic herbicide-tolerance characteristic is DART. Another embodiment described herein is a seed of or capable of producing a plant having the auxinic herbicide-tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, with the proviso that the seed is other than a Sinapis arvensis seed. In one aspect, the auxinic herbicide-tolerance characteristic is DART. Another embodiment is a seed of or capable of producing a plant with the auxinic herbicide-tolerance characteristic. In one aspect, the auxinic herbicide-tolerance characteristic is DART. In another aspect, the seed has, disposed on a surface thereof, a composition comprising at least one agronomically acceptable ingredient. In one aspect, the ingredient is at least one agronomically acceptable herbicide, fungicide, nematicide, or insecticide, or a combination thereof. In another aspect, the insecticide comprises at least one anti-coleopteran agent, anti- hemipteran agent, anti-lepidopteran agent, or a combination thereof. Another embodiment is a method for treating a seed with an agronomically acceptable composition. In one aspect, the method comprises contacting the seed with an agronomically acceptable composition. In another aspect, the agronomically acceptable composition comprises an auxinic herbicide. Another embodiment described herein is a cell of a plant having the auxinic herbicide- tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, with the proviso that the cell is of a monocot or dicot species other than Sinapis arvensis. In one aspect, the auxinic herbicide-tolerance characteristic is DART. Another embodiment described herein is a plant product produced from a plant having the auxinic herbicide-tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R. Another embodiment is a plant product of a plant comprising the auxinic herbicide-tolerance characteristic is DART. In one-aspect plant products, include, but are not limited to, seeds, hulled or dehulled grain, oil, and meal. In other aspects, plant products include whole plant, aerial plant part, stem, and/or leaf feed, fodder, and forages. In another aspect, the plant product is grain, meal, or oil. One embodiment described herein is a method for controlling weeds at a locus for growth of a plant. In one aspect, the locus for growth of a plant is a field. In one aspect, the method comprises: applying a composition comprising an auxinic herbicide to the locus, wherein the plant has an auxinic herbicide-tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R. In one aspect, the auxinic herbicide-tolerance characteristic is the DART trait. Another embodiment described herein is a method for controlling weeds in a field by application of an auxinic herbicide without significantly inhibiting the growth of a Brassica plant, the method comprising: (a) providing a Brassica plant or seed of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R; and (b) applying an herbicide composition comprising an effective amount of an auxinic herbicide: (i) to the field, followed by planting of said plant or seed in therein; (ii) to the field, during or after planting of said seed therein; (iii) to the plant in said field and to weeds in the vicinity of the plant; (iv) to said seed, followed by planting of said seed in the field; or (v) to a plant by the seed after it has been planted in the field, and to weeds in the vicinity of the plant; wherein the effective amount of the auxinic herbicide would significantly inhibit the growth of a corresponding wild-type variety of said Brassica plant; thereby controlling weeds. In one aspect, the plant is a Brassicajuncea, B. napus, or B. rapa plant. In another aspect, the plant is a canola plant. In another aspect, the step of applying comprises performing post-emergent treatment of the plant by applying an herbicide composition, comprising auxinic herbicide(s), to the plant and its immediate vicinity, at a dose rate of about 10 to about 5000 grams active ingredient per hectare (ai/ha). In another aspect, the step of applying comprises performing pre-emergent treatment, or 0 to 30 days-pre-planting treatment, of the plant by applying an herbicide composition, comprising auxinic herbicide(s), to the seed planting locus thereof and its immediate vicinity, at a dose rate of about 10 to about 5000 g ai/ha. Another embodiment described herein is a method for controlling weeds in a crop field by use of an auxinic herbicide without significantly inhibiting the growth of a crop plant, the method comprising: (a) providing a seed-treatment-treated seed comprising the nucleic acid of claim 41, the expression of said nucleic acid conferring to the plant or seed tolerance to an auxinic herbicide, the seed treatment comprising an auxinic herbicide; and (b) planting said treated seed in the field. Another embodiment described herein is a method for producing an auxinic herbicide- tolerant progeny plant. In one aspect, the method comprises: crossing the parent plant with an auxinic herbicide tolerant plant having the auxinic herbicide-tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT- 0 1 SA2-R, or DT-01 BC8SA2-R, with the proviso that the plant is a monocot or dicot species other than Sinapis arvensis, to introduce the auxinic herbicide-tolerance characteristic into the germplasm of the progeny plant, wherein the progeny plant has increased tolerance to the auxinic herbicide relative to the parent plant. Another embodiment described herein is a method for producing an auxinic herbicide- tolerant progeny plant. In one aspect, the method comprises: (a) providing a parent plant of a desired line; and (b) crossing the parent plant with any one of the plants according of claims 1-4 to introduce the auxinic herbicide-tolerance characteristic into the germplasm of the progeny plant, wherein the progeny plant thereby has increased tolerance to an auxinic herbicide relative to the parent plant. In another aspect, the method further comprises introgressing the auxinic herbicide-tolerance characteristic of the progeny plant through traditional plant breeding techniques to obtain a descendent plant having the auxinic herbicide-tolerance characteristic. In another aspect, the parent plant comprises at least one herbicide tolerant (HT) mutant AHASL gene. In another aspect, the parent plant is a dicot. In another aspect, the parent plant is a Brassica or Rhaphanus plant. In another aspect, the parent plant is a Brassicajuncea, B. napus, or B. rapa plant. In yet another aspect, the parent plant is a canola plant. Another embodiment described herein is a method for identifying an auxinic herbicide tolerant plant, or plant part thereof. In one aspect, the method comprises: (a) providing biological material from a plant comprising the DART trait; (b) performing PCR, hybridization testing, or sequencing of said nucleic acid in said biological material to determine if said plant comprises the DART trait; and (c) identifying, based on the results of step (b), that the plant comprises the DART trait. In one aspect the plant comprises: (i) any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT- 0 1 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent Deposit Designation Numbers PTA-

120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA-1 1213, and PTA-1 1214, respectively; (ii) a mutant, recombinant, or a genetically engineered derivative of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT- 0 1 BC8SA2-R and that expressed the DART trait thereof; or (iii) a plant that is a progeny of at least any one of the plants of (i)-(ii) and that expressed the DART trait thereof. In another aspect, the biological material comprises a Brassica or a Raphanobrassica plant, plant part thereof, seed, or cell. BRIEF DESCRIPTION OF THE DRAWINGS Figure 1: Photographic results of an in vitro, whole plant kill curve. Figure 2 : Photographic images of a trichome trait observed in parent lines and Fl hybrid plant. Figure 3 : Dendrogram generated from SNP analysis. Figure 4 : Backcrossing scheme illustrating a procedure for development of wild mustard near-isogenic-lines (NILs) with auxinic herbicide-R (resistance) and herbicide-S (susceptibility). Figure 5 : Photographic images of wild mustard plants 1 days after planting (DAP). A and B indicate S and R plants, respectively. Figure 6 : Bar chart presenting leaf area (cm2) of first true leaf of wild mustard (31 DAP).

S : Parental-S; R : Parental-R; BC-Pl and BC-P2 are BC4F 1 progeny with larger leaf area and smaller leaf area, respectively. Vertical bars represent SEM (Standard Error of the Means). Figure 7 : Nucleotide sequence of markers 1-6 (M1-M6) (SEQ ID NOs:l-6, respectively). Figure 8 : Linkage map of wild mustard with markers 1-6 (M1-M6). R : Auxinic herbicide resistance genetic locus. Figure 9 : Ovule/embryo rescue procedure to produce hybrids between B.juncea, B. rapa, and S. arvensis. A, represents the immature silique cultured on media (A or B); B represents ovule excised from the siliques and C denotes the hybrid plant regeneration form the ovule. Figure 10: Production of hybrids between B. juncea, B. rapa, and S. arvensis. A, B, C represent B. juncea, B. rapa, and S. arvensis, respectively. D, Dl, and E, El illustrate hybrids produced via embryo rescue from crosses between B. juncea x S. arvensis and B. rapa x S. arvensis, respectively. Note, the hybrids exhibiting intermediate characteristics of the parents. Figure 11: Plant response to dicamba, 200 g acid equivalents/hectare (ae/ha), 5 weeks after treatment. A, B, C represent B. juncea, B. rapa, and S. arvensis, respectively. D and E indicate hybrids produced by crossing B. juncea S. arvensis and B. rapa x S. arvensis, respectively. DETAILED DESCRIPTION Definitions As used herein, "tolerant" or "herbicide-tolerant" indicates a plant or plant part thereof capable of growing in the presence of an amount of herbicide that normally causes growth inhibition or phytotoxicity in a non-herbicide-tolerant (e.g., a wild-type) plant or portion thereof. Levels of herbicide that normally inhibit growth of a non-tolerant plant are known and readily determined by those skilled in the art. Examples include the quantity of herbicide or rate of application recommended by herbicide manufacturers. The maximum level or rate of herbicide application is the amount of herbicide that would normally inhibit the growth or cause phytotoxicity of a non-herbicide tolerant plant (e.g., a wild type plant or weed). As described herein, the terms "herbicide-tolerant" and "herbicide-resistant" are used interchangeably and are intended to have an equivalent meaning and an equivalent scope. Similarly, the terms "herbicide-tolerance" and "herbicide-resistance" are used interchangeably and are intended to have an equivalent meaning and an equivalent scope. Similarly, the terms "tolerant" and "resistant" are used interchangeably and are intended to have an equivalent meaning and an equivalent scope. As used herein, the phrases "auxinic herbicide tolerance trait" or "auxinic herbicide tolerance characteristic" are used interchangeably and are intended to have an equivalent meaning and an equivalent scope. As used herein, the auxinic herbicide tolerance trait is the auxinic herbicide tolerance phenotype present in any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, and is referred to herein as "DART." In various embodiments, the auxinic herbicide tolerance trait is effected by a functional expression product of a nucleic acid comprising the nucleotide sequence of any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, or a synonymous codon substituted variant thereof. For example, the expression product may be a polypeptide comprising the amino acid sequence of any one of SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 81, or a mature form thereof, or a conservatively substituted variant thereof. In some embodiments, a nucleotide sequence encoding the amino acid sequence is at least 90% homologous to any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, or a synonymous codon substituted variant thereof. In other embodiments, the encoded amino acid sequence is 70% homologous to any one of SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 81, or a mature form thereof. As used herein, the phrases "without inhibiting the growth" or "without significantly inhibiting the growth" are used to mean that a dicamba-treated plant comprising the DART trait will exhibit a dicamba tolerance of 75% or more, as determined 5 weeks after treatment with an effective amount of the herbicide. Yet, that amount of dicamba will inhibit the growth of, or can even result in the death of, a wild type variety of the plant, i.e. one lacking the DART trait. As used herein in regard to useful in various embodiments hereof, terms such as auxinic herbicide, AHAS inhibitor, acetyl-Coenzyme A carboxylase (ACCase) inhibitor, PPO inhibitor, EPSPS inhibitor, imidazolinone, sulfonylurea, and the like, refer to those agronomically acceptable herbicide active ingredients (A.I.s) recognized in the art. Similarly, terms such as fungicide, nematicide, pesticide, and the like, refer to other agronomically acceptable A.I.s recognized in the art. When used in reference to a particular mutant enzyme or polypeptide, terms such as herbicide tolerant (HT) and herbicide tolerance refer to the ability of such enzyme or polypeptide to perform its physiological activity in the presence of an amount of an herbicide A.I. that would normally inactivate or inhibit the activity of the wild-type (non-mutant) version of said enzyme or polypeptide. For example, when used specifically with regard to an AHAS enzyme, or AHASL polypeptide, it refers specifically to the ability to tolerate an AHAS-inhibitor. Classes of AHAS-inhibitors include sulfonylureas, imidazolinones, triazolopyrimidines, sulfonylaminocarbonyltriazolinones, and pyrimidinyloxy[thio]benzoates. In some embodiments in which an imidazolinone herbicide is to be used, the AHASL is preferably one that comprises at least one herbicide tolerance mutation located at amino acid residue position 122, 205, 574, or 653 {Arabidopsis thaliana AHASL numbering); and in some embodiments in which a sulfonylurea herbicide is to be used, the AHASL is preferably one that comprises at least one herbicide tolerance mutation located at amino acid residue position 197 or 574 {Arabidopsis thaliana AHASL numbering). As used herein, "recombinant," when referring to nucleic acid or polypeptide, indicates that such material has been altered as a result of human application of a recombinant technique, such as by polynucleotide restriction and ligation, by polynucleotide overlap-extension, or by genomic insertion or transformation. A gene sequence open reading frame is recombinant if that nucleotide sequence has been removed from its natural context and cloned into any type of artificial nucleic acid vector. The term recombinant also can refer to an organism having a recombinant material, e.g., a plant that comprises a recombinant nucleic acid can be considered a recombinant plant. The term "transgenic plant" refers to a plant that comprises a heterologous polynucleotide that has been inserted by the use of a recombinant DNA technique. Preferably, the heterologous polynucleotide is stably integrated within the genome such that the polynucleotide is passed on to successive generations. The heterologous polynucleotide may be integrated into the genome alone or as part of a recombinant expression cassette. "Transgenic" is used herein to refer to any cell, cell line, callus, tissue, plant part, or plant, the genotype of which has been so altered by the presence of the heterologous nucleic acid introduced by the use of a recombinant DNA technique, including those transgenic organisms or cells initially so altered, as well as those created by crosses or asexual propagation from the initial transgenic organism or cell. A transgenic organism can also be referred to herein as a "recombinant" organism. The term "transgenic" as used herein is not intended to encompass the alteration of the genome (chromosomal or extra-chromosomal) by conventional plant breeding methods (e.g., crosses) or by naturally occurring events such as, e.g., self-fertilization, random cross-fertilization, non- recombinant viral infection, non-recombinant bacterial transformation, non-recombinant transposition, or spontaneous mutation. As used herein, "mutagenized" refers to an organism or DNA thereof having alteration(s) in the biomolecular sequence of its native genetic material as compared to the sequence of the genetic material of a corresponding wild-type organism or DNA, wherein the alteration(s) in genetic material were induced and/or selected by a non-recombinant technique through human action. Any mutagenesis method known in the art can be used to induce mutations. Methods of inducing mutations can induce mutations in random positions in the genetic material or can induce mutations in specific locations in the genetic material (i.e., can be directed mutagenesis techniques), such as by use of a genoplasty technique. Specific examples of mutagenesis, followed by selection with an herbicide (e.g., auxinic herbicides), in order to obtain a mutagenized organism having an herbicide tolerance trait or DNA encoding such trait, include, but are not limited to: tissue culture of plant cells (e.g., calli) to induce tissue culture mutagenesis, treatment of plant cells with a chemical mutagen, or treatment of plant cells with radiation (e.g., gamma rays, X-rays, or subatomic particles). As used herein, "chimeric" biomolecule is a non-naturally-occurring biomolecule that contains sequence segments from two different species, attached to each other. In some embodiments, a non-naturally-occurring, chimeric DNA can be prepared by artificially cross- pollinating plants of different species. For example, a chimeric nucleic acid can comprise nucleotide sequence from a first species attached to a nucleotide sequence from a second species. The different sequence segments are directly attached one to another. Thus, e.g., a Sinapis arvensis polynucleotide segment attached to a Brassica polynucleotide segment will together constitute a chimeric nucleic acid. Similarly, chimeric polypeptides can also be formed by expression of a chimeric nucleic acid coding sequence. As used herein, a non-transgenic, non- naturally-occurring, chimeric DNA, prepared by artificially cross-pollinating plants of different species, is one that in nature is not transmitted to progeny without having been artificially propagated at its inception, e.g., by embryo rescue and/or by tissue culture and plantlet regeneration. As used herein, a "genetically modified organism" (GMO) is an organism whose genetic characteristics contain alteration(s) that were produced by human effort causing transfection that results in transformation of a target organism with genetic material from another or "source" organism, or with synthetic or modified-native genetic material, or an organism that is a descendant thereof that retains the inserted genetic material. The source organism can be of a different type of organism (e.g., a GMO plant can contain bacterial genetic material) or from the same type of organism (e.g., a GMO plant can contain genetic material from another plant). As used herein with regard to plants and other organisms, "recombinant," "transgenic," and "GMO" are considered synonyms and indicate the presence of genetic material from a different source. As used herein, "wild-type" or "corresponding wild-type plant" means the typical form of an organism or its genetic material, as it normally occurs, as distinguished from, e.g., mutagenized and/or recombinant forms. As used herein, "descendant" refers to any generation plant. As used herein, "progeny" refers to a first generation plant. The term "seed" comprises seeds of all types, such as, for example, true seeds, caryopses, achenes, fruits, tubers, seedlings and similar forms. In the context of Brassica and Sinapis species, "seed" refers to true seed(s) unless otherwise specified. The seed used can be seed of the useful plants mentioned above, but also the seed of transgenic plants or plants obtained by traditional breeding methods. Examples of traditional breeding methods can include cross breeding, selfmg, back-crossing, embryo rescue, in-crossing, out-crossing, inbreeding, selection, asexual propagation, and other traditional techniques as are known in the art. As used herein, the term "derived from," unless otherwise specified, indicates that a particular thing (e.g., plant, seed, etc.) or group of things has originated from the source specified, but has not necessarily been obtained directly from the specified source. Although exemplified with reference to specific plants or plant varieties, e.g., canola/oilseed rape (OSR) (B. napus), wild mustard {Sinapsis arvensis), and their hybrids, in various embodiments, the presently described methods using auxinic herbicides can be employed with a variety of commercially valuable plants including, but not limited to, auxinic herbicide- tolerant Brassica species, such as B. oleracea, B. rapa, B. nigra, and B. juncea. Auxinic herbicide-tolerant plant lines described as useful herein can be employed in weed control methods either directly or indirectly, i.e., either as crops for herbicide treatment or as auxinic herbicide-tolerance trait donor lines for development, as by traditional plant breeding, to produce other varietal and/or hybrid crops containing such trait. All such resulting variety or hybrids crops, containing the ancestral auxinic herbicide-tolerance trait can be referred to herein as progeny of the ancestral, auxinic herbicide-tolerant line(s). Such resulting plants can be said to "retain the herbicide tolerance characteristic(s) of the ancestral plant, i.e., meaning that they possess and express the ancestral genetic molecular components responsible for the trait. In the case of Brassica A-, B-, and C-genome-located auxinic herbicide traits, these can be bred into Brassica species having a corresponding genome, e.g., B. napus (AACC), B.juncea (AABB), B. oleracea (CC), B. rapa (AA), B. nigra (BB), B. carinata (BBCC), and Raphanobrassica varieties that are progeny of a cross between any of the foregoing and a Raphanus spp., e.g., Raphanobrassica var. 'rabbage' (RRCC) from B. oleracea Raphanus sativus or Raphanobrassica var. 'raparadish' (RRAA) from B. rapa Raphanus sativus. Among these, B. napus, B. rapa, and B. juncea are of particular interest, with B. napus being preferred in some embodiments. For example, in one embodiment, the auxinic herbicide-tolerant plants described herein can be employed as auxinic herbicide-tolerance characteristic donor lines to produce other varietal and/or hybrid crops containing such a characteristic. In some embodiments, the selected Brassica will be a canola variety. Non-limiting examples of Brassica taxa that can provide useful canola varieties include B. rapa (esp. of Oleifera group), B. napus (esp. of Napus group), and B.juncea (esp. of Juncea group). As used herein, a "canola" plant, line, or variety is a Brassica plant, line, or variety that has been bred or otherwise engineered to produce seed oil that, compared to seed oil of members of the parent line or of a wild-type line, is reduced in erucic acid content, or in both erucic acid and glucosinolate content. In some embodiments, the canola will be a food-grade canola, i.e., wherein: (1) the seed oil thereof contains 2% by weight or less of its fatty acids as erucic acid, preferably less than or about 1%, 0.5% or, 0.2% thereof, or essentially 0%> thereof; and (2) the oil-removed, dry seed meal contains less than 30 micromoles per gram of any one or any mixture of 3-butenyl glucosinolate, 2-hydroxy-3-butenyl glucosinolate, 4-pentenyl glucosinolate, and 2-hydroxy-4- pentenyl glucosinolate, preferably less than or about 20, 15, 10, 5, or 2 umol/g thereof, or essentially 0 µιηοΐ g thereof. In some embodiments, the canola plant, line, or variety is one in which the indole-glucosinolate content, e.g., the content of glucobrassicin (3-indolylmethyl- glucosinolate), 4-hydroxy-glucobrassicin, 4-methoxy-glucobrassicin, and/or neoglucobrassicin (1-methoxy-glucobrassicin), in the seed meal is modified compared to that in seed meal of the parent plant or a wild-type, line, or variety; in some embodiments, such a canola can also be a food-grade canola. Where the seed meal is intended for use as livestock feed, in some embodiments, the canola is preferably selected to be one in which such meal indole- glucosinolate content is decreased compared to that of the parent plant or of a wild-type plant. As used herein, the term "canola" can appear per se or in combination terms such as "canola/OSR" and "canola/oilseed rape;" the combined term referring to oilseed Brassica canola varieties. The term "oilseed rape," when not modified by combination with the term "canola" is understood as encompassing both canola and non-canola varieties of oilseed Brassicas, i.e., Brassicas that express useful seed oils. Seed oils can be removed from the seed by crushing, pressing, solvent extraction, any other techniques known useful in the art. One embodiment described herein is an auxinic herbicide-tolerant plant or plant part thereof, wherein the plant is a plant of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT- 0 1 BC4Bn#13-l, or DT-01 BC5Bn#13-l-18. In some embodiments, the herbicide-tolerance characteristic of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, and DT-01 BC5Bn#13-l-18 are the same herbicide tolerance-characteristic. In other embodiments, the herbicide-tolerance characteristic of lines DT-01 SA2-R and DT-01 BC8SA2-R are the same herbicide tolerance-characteristic. In one aspect, the herbicide-tolerance characteristic is DART. A deposit of a representative sample of seeds of each of lines DT-01 BC3Bn#13, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R was made by BASF Plant Science, 26 Davis Drive, Research Triangle Park, NC 27709, United States of America and University of Guelph, Unit 102, 150 Research Lane, Guelph, Ontario, NIG 4T2, Canada with the ATCC, 10801 University Blvd., Manassas, Virginia, 201 10, United States of America on July 16, 2010; a deposit of a representative sample of seeds of line DT-01 BC4Bn#13-l was made by BASF

Plant Science and University of Guelph with the ATCC on August 30, 201 1; and a deposit of a representative sample of seeds of line DT-01 Cyc2 BNS4 was made by BASF Plant Science and University of Guelph with the ATCC on January 15, 2013. The depositors, BASF Plant Science and University of Guelph, hereby authorize BASF SE and University of Guelph to use the deposited seeds. The deposits will be maintained at the ATCC depository under the terms of the Budapest Treaty on the International Recognition of the Deposit of Microorganisms for the Purposes of Patent Procedure. Additionally, Applicants have satisfied all the requirements of 37 C.F.R. §§ 1.801-1.809, including providing an indication of the viability of the sample. Applicants impose no restrictions on the availability of the deposited material from the ATCC after the issuance of a patent from this application. Applicants have no authority to wave any restrictions imposed by law on the transfer of biological material or its transportation in commerce. Applicants do not waive any infringement of Applicants' rights granted under any patent issued from this application. Additional deposits will be made at the ATCC, as needed, to ensure availability subject to the conditions described herein for the respective deposits. One embodiment described herein is the DART auxinic herbicide tolerance trait. Another embodiment is a nucleic acid comprising: (a) a chimeric polynucleotide comprising both a Sinapis arvensis polynucleotide portion and a Brassica polynucleotide portion, wherein said chimeric polynucleotide encodes the DART trait of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent Deposit Designation Numbers PTA-

120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA-1 1213, and PTA-1 1214, respectively; or (b) a mutagenized or recombinant polynucleotide encoding the DART trait of any one of lines DT- 0 1 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2- R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent Deposit Designation Numbers PTA-

120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA-1 1213, and PTA-1 1214, respectively. In one aspect, the nucleic acid is isolated. In another aspect, the nucleic acid comprises (a) a nucleotide sequence according to any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80; or (b) a nucleotide sequence encoding a polypeptide comprising an amino acid sequence according to any one of SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 81, respectively; or a mature form thereof. In other aspects, the nucleic acids can be 90% homologous to any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, or a synonymous codon substituted variant thereof. In other aspects, the encoded amino acid sequence is 70%> homologous to any one of SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 81, or a mature form thereof. In one embodiment the nucleic acid comprises: (a) the nucleotide sequence of SEQ ID NO: 8; (b) a nucleotide sequence encoding a translation elongation factor EF1A/initiation factor family polypeptide comprising the amino acid sequence of SEQ ID NO:9, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:8 and encodes an auxinic-herbicide-tolerant, functional, translation elongation factor EF1A/initiation factor family polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, translation elongation factor EF1A/initiation factor family polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:9. In another embodiment the nucleic acid comprises: (a) the nucleotide sequence of SEQ ID NO: 12; (b) a nucleotide sequence encoding a KAT2 peroxisomal 3-ketoacyl-CoA thiolase 3 polypeptide comprising an amino acid sequence according to SEQ ID NO: 13, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO: 12 and encodes an auxinic-herbicide-tolerant, functional, KAT2 peroxisomal 3-ketoacyl-CoA thiolase 3 polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, KAT2 peroxisomal 3-ketoacyl-CoA thiolase 3 polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO: 13. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO: 16; (b) a nucleotide sequence encoding a calmodulin 5-like polypeptide comprising an amino acid sequence according to SEQ ID NO: 17, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO: 16 and encodes an auxinic- herbicide-tolerant, functional, calmodulin 5-like polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, calmodulin 5-like polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO: 17. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO: 18; (b) a nucleotide sequence encoding an ARF21 transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO: 19, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO: 18 and encodes an auxinic-herbicide-tolerant, functional, ARF21 transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, ARF21 transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO: 19. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:22; (b) a nucleotide sequence encoding a TPR3 transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:23, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:22 and encodes an auxinic- herbicide-tolerant, functional, TPR3 transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, TPR3 transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:23. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:26; (b) a nucleotide sequence encoding a Chloroplast Trans-Membrane, - Associated Protein- 1 (cpTAAP-1) polypeptide comprising an amino acid sequence according to SEQ ID NO:27, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:26 and encodes an auxinic-herbicide-tolerant, functional, Chloroplast Trans-Membrane, Auxin-Associated Protein- 1 (cpTAAP-1) polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, Chloroplast Trans- Membrane, Auxin-Associated Protein- 1 (cpTAAP-1) polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:27. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:30; (b) a nucleotide sequence encoding a Endo-Mitochondrial, Auxin-Associated Protein- 1 (mtAAP-1) polypeptide comprising an amino acid sequence according to SEQ ID NO:31, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:30 and encodes an auxinic-herbicide-tolerant, functional, Endo-Mitochondrial, Auxin-Associated Protein- 1 (mtAAP-1) polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, Endo-Mitochondrial, Auxin-Associated Protein- 1 (mtAAP-1) polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:31. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:34; (b) a nucleotide sequence encoding a zinc-binding ribosomal protein family polypeptide comprising an amino acid sequence according to SEQ ID NO:35, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:34 and encodes an auxinic-herbicide-tolerant, functional, zinc-binding ribosomal protein family polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, zinc-binding ribosomal protein family polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:35. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:36; (b) a nucleotide sequence encoding a SAUR-like auxin-responsive protein family transcription factor polypeptide comprising an amino acid sequence according to SEQ ID

NO:37, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:36 and encodes an auxinic-herbicide-tolerant, functional, SAUR-like auxin- responsive protein family transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, SAUR-like auxin-responsive protein family transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:37. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:40; (b) a nucleotide sequence encoding a HTA13 histone polypeptide comprising an amino acid sequence according to SEQ ID NO:41, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:40 and encodes an auxinic-herbicide- tolerant, functional, HTA13 histone polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, HTA13 histone polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:41. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:44; (b) a nucleotide sequence encoding a Knotted 1 like (KNAT4) transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:45, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:44 and encodes an auxinic-herbicide-tolerant, functional, Knotted 1 like (KNAT4) transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, Knotted 1 like (KNAT4) transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:45. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:48; (b) a nucleotide sequence encoding a Zinc-Finger-Like, Auxin-Associated Protein- 1 (zFAAP-1) polypeptide comprising an amino acid sequence according to SEQ ID NO:49, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:48 and encodes an auxinic-herbicide-tolerant, functional, Zinc-Finger-Like, Auxin- Associated Protein- 1 (zFAAP-1) polypeptide; or (d) a nucleotide sequence encoding an auxinic- herbicide-tolerant, functional, Zinc-Finger-Like, Auxin-Associated Protein- 1 (zFAAP-1) polypeptide comprising an amino acid sequence at least 70%> identical to SEQ ID NO:49. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:52; (b) a nucleotide sequence encoding a Alba DNA/RNA-binding protein transcription factor polypeptide comprising an amino acid sequence according to SEQ ID

NO:53, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:52 and encodes an auxinic-herbicide-tolerant, functional, Alba DNA/RNA-binding protein transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic- herbicide-tolerant, functional, Alba DNA/RNA-binding protein transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:53. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:56; (b) a nucleotide sequence encoding an IAA16 transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:57, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:56 and encodes an auxinic- herbicide-tolerant, functional, IAA16 transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, IAA16 transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:57. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:62; (b) a nucleotide sequence encoding an IAA12 bodenlos/monopteros transcription factor polypeptide comprising an amino acid sequence according to SEQ ID

NO:63, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:62 and encodes an auxinic-herbicide-tolerant, functional, IAA12 bodenlos/monopteros transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, IAA12 bodenlos/monopteros transcription factor polypeptide comprising an amino acid sequence at least 70%> identical to SEQ ID NO:63. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:66; (b) a nucleotide sequence encoding an RUB1 polypeptide comprising an amino acid sequence according to SEQ ID NO:67, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:66 and encodes an auxinic-herbicide- tolerant, functional, RUB1 polypeptide; or (d) a nucleotide sequence encoding an auxinic- herbicide-tolerant, functional, RUB1 polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:67. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:76; (b) a nucleotide sequence encoding a CAM7 transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:77, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:76 and encodes an auxinic- herbicide-tolerant, functional, CAM7 transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, CAM7 transcription factor polypeptide comprising an amino acid sequence at least 70%> identical to SEQ ID NO:77. In another embodiment the nucleic acid comprises: (a) a nucleotide sequence according to SEQ ID NO:80; (b) a nucleotide sequence encoding a cytochrome P450 CYP83A1 polypeptide comprising an amino acid sequence according to SEQ ID NO:81, or a mature form thereof; (c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO: 80 and encodes an auxinic-herbicide-tolerant, functional, cytochrome P450 CYP83A1 polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, cytochrome P450 CYP83Alpolypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:81. Another embodiment described herein is an auxinic herbicide-tolerant plant or plant part thereof having the auxinic herbicide-tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, with the proviso that the plant is a monocot or dicot species other than Sinapis arvensis. In one aspect, the auxinic herbicide-tolerance characteristic is DART. In other embodiments, the auxinic herbicide-tolerant plant is a descendent of a member of any one of DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l- 18, DT-01 SA2-R, or DT-01 BC8SA2-R. In one aspect, the descendant was obtained by traditional plant breeding from said member. In another aspect, the plant is a monocot or dicot species other than a Sinapis species. In one aspect, the auxinic herbicide-tolerant plant is a Brassica species, such as B. napus, B. oleracea, B. rapa, B. nigra, and B.juncea. One aspect described herein is a progeny or a descendant of an auxinic herbicide-tolerant plant, as well as seeds derived from the auxinic herbicide-tolerant plants, and cells derived from the auxinic herbicide-tolerant plants. In some embodiments, plant cells described herein are capable of regenerating a plant or plant part. In other embodiments, plant cells are not capable of regenerating a plant or plant part. Examples of cells not capable of regenerating a plant include, but are not limited to, endosperm, seed coat (testa and pericarp), and root cap. One embodiment described herein is a plant product prepared from the auxinic herbicide- tolerant plants hereof. In some embodiments, examples of plant products include, without limitation, grain, oil, and meal. In one embodiment, a plant product is canola grain (e.g., grain suitable for use as feed or for processing), canola oil (e.g., oil suitable for use as food or biodiesel), or canola meal (e.g., meal suitable for use as feed). In other embodiments, auxinic herbicide-tolerant plant lines described as useful herein can be employed as auxinic herbicide-tolerance characteristic(s) donor lines for development, as by traditional plant breeding, to produce other varietal and/or hybrid crops containing such trait. All such resulting variety or hybrids crops, containing the ancestral auxinic herbicide-tolerance characteristic or characteristic can be referred to herein as progeny or descendant of the ancestral, auxinic herbicide-tolerant line(s). One embodiment described herein is a method for producing an auxinic herbicide- tolerant progeny plant, the method comprising: crossing a parent plant with an auxinic herbicide- tolerant plant having an auxinic herbicide-tolerance characteristic to introduce the auxinic herbicide-tolerance characteristic into the germplasm of the progeny plant, wherein the progeny plant has increased tolerance to the auxinic herbicide relative to the parent plant. In some embodiments, the auxinic herbicide-tolerant plant that is crossed with the parent plant is a plant of line DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R. In other embodiments, the method further comprises the step of introgressing the auxinic herbicide-tolerance characteristic of the progeny plant through traditional plant breeding techniques to obtain a descendent plant having the auxinic herbicide-tolerance characteristic. One example of a progeny plant is an interspecies crossed between different Brassica species. Another example of a progeny plant is an intergeneric cross between Raphanus sativus with Brassica oleracea to provide a Raphanobrassica. For example, in the case of Brassica A-, B-, and C-genome auxinic herbicide trait(s), these can be bred into Brassica species having a corresponding genome, e.g. : B. napus (AACC), B. juncea (AABB), B. oleracea (CC), B. rapa (AA), B. nigra (BB), B. carinata (BBCC), and Raphanobrassica varieties that are progeny of a cross between any of the foregoing and a Raphanus spp., e.g., Raphanobrassica var. 'rabbage' (RRCC) from B. oleracea Raphanus sativus or Raphanobrassica var. 'raparadish' (RRAA) from B. rapa Raphanus sativus. Among these, in some embodiments, B. napus, B. rapa, and B.juncea are of particular interest, with B. napus being preferred in other embodiments. In some embodiments, traditional plant breeding is employed whereby the auxinic herbicide-tolerant trait is introgressed to obtain a descendent plant resulting therefrom, wherein the descendent plant is tolerant to an auxinic herbicide. In one embodiment, the descendent plant has the auxinic herbicide-tolerance characteristic of line DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R. Other embodiments described herein are plants, including those plants which, in addition to being auxinic herbicide-tolerant, have been subjected to further genetic modifications by breeding, mutagenesis or genetic engineering, e.g., have been rendered tolerant to applications of specific other classes of herbicides, such as AHAS inhibitors; bleaching herbicides such as hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors or phytoene desaturase (PDS) inhibitors; enolpyruvyl shikimate 3-phosphate synthase (EPSPS) inhibitors such as ; glutamine synthetase (GS) inhibitors such as ; protoporphyrinogen-IX oxidase (PPO) inhibitors; lipid biosynthesis inhibitors such as ACCase inhibitors; or oxynil (i.e., or ioxynil) herbicides as a result of conventional methods of breeding or genetic engineering, Thus, auxinic herbicide tolerant plants can be made resistant to multiple classes of herbicides through multiple genetic modifications, such as resistance to both glyphosate and glufosinate or to both glyphosate and a herbicide from another class such as HPPD inhibitors, AHAS inhibitors, or ACCase inhibitors. These herbicide resistance technologies are, for example, described by Arias et al. (2005); Inui and Ohkawa (2005); Li and Nicholl (2005); Matringe et al. (2005); Tan et al. (2005); Behrens et al. (2007); Charles et al. (2007); Dill et al. (2008); Green et al. (2008); Green (2009), and references quoted therein. For example, in some embodiments, auxinic herbicide-tolerant plants may be tolerant to ACCase inhibitors, such as "dims" (e.g., cycloxydim, , clethodim, or tepraloxydim), "fops" (e.g., clodinafop, diclofop, , haloxyfop, or quizalofop), and "dens" (such as pinoxaden); to EPSPS inhibitors, such as glyphosate; to PPO inhibitors, such as saflufenacil; and to GS inhibitors, such as glufosinate. In addition to these classes of inhibitors, auxinic herbicide- tolerant plants may also be tolerant to herbicides having other modes of action, for example, /carotenoid pigment inhibitors, cell membrane disruptors, photosynthesis inhibitors, cell division inhibitors, root inhibitors, shoot inhibitors, and combinations thereof. Such tolerance traits may be expressed, e.g., as mutant ACCase proteins, mutant EPSPS proteins, or mutant glutamine synthetase proteins; or as mutant native, inbred, or transgenic aryloxyalkanoate dioxygenase (AAD or DHT), haloarylnitrilase (BXN), 2,2-dichloropropionic acid dehalogenase (DEH), glyphosate-N-acetyltransferase (GAT), glyphosate decarboxylase (GDC), glyphosate oxidoreductase (GOX), glutathione-S-transferase (GST), phosphinothricin acetyltransferase (PAT or bar), or cytochrome P450 (CYP450) proteins having an herbicide-degrading activity. Auxinic herbicide-tolerant plants hereof can also be stacked with other traits including, but not limited to, pesticidal traits such as Bt Cry and other proteins having pesticidal activity toward coleopteran, lepidopteran, nematode, or other pests; nutrition or nutraceutical traits such as modified oil content or oil profile traits, high protein or high amino acid concentration traits, and other trait types known in the art. Furthermore, in other embodiments, auxinic herbicide-tolerant plants (e.g., oilseed rape (canola)) are also covered which are, by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such characteristic, rendered able to synthesize one or more insecticidal proteins, especially those known from the bacterial genus Bacillus, particularly from Bacillus thuringiensis, such as δ-endotoxins, e.g., CrylA(b), CrylA(c), CrylF, CryIF(a2), CryIIA(b), CrylllA, CrylllB(bl) or Cry9c; vegetative insecticidal proteins (VIP), e.g., VIP1, VIP2, VIP3 or VIP3A; insecticidal proteins of bacteria colonizing nematodes, e.g., Photorhabdus spp. or Xenorhabdus spp.; toxins produced by , such as scorpion toxins, arachnid toxins, wasp toxins, or other -specific neurotoxins; toxins produced by fungi, such streptomycete toxins; plant lectins, such as pea or barley lectins; agglutinins; proteinase inhibitors, such as trypsin inhibitors, serine protease inhibitors, patatin, cystatin or papain inhibitors; ribosome-inactivating proteins (RIP), such as ricin, maize-RIP, abrin, luffin, saporin or bryodin; steroid metabolism enzymes, such as 3-hydroxy-steroid oxidase, ecdysteroid-IDP- glycosyl-transferase, cholesterol oxidases, ecdysone inhibitors or HMG-CoA-reductase; ion channel blockers, such as blockers of sodium or calcium channels; juvenile hormone esterase; diuretic hormone receptors (helicokinin receptors); stilben synthase, bibenzyl synthase, chitinases or glucanases. As described herein, these insecticidal proteins or toxins are to be understood expressly also as pre-toxins, hybrid proteins, truncated or otherwise modified proteins. Hybrid proteins are characterized by a new combination of protein domains, (see, e.g., WO 02/015701). Further examples of such toxins or genetically modified plants capable of synthesizing such toxins are disclosed, e.g., in EP-A 374 753, WO 93/007278, WO 95/34656, EP-A 427 529, EP-A 451 878, WO 03/18810 und WO 03/52073. The methods for producing such genetically modified plants are generally known to the person skilled in the art and are described, e.g., in the publications mentioned above. These insecticidal proteins contained in the genetically modified plants impart to the plants producing these proteins tolerance to harmful pests from all taxonomic groups of , especially to beetles (Coeloptera), two-winged (Diptera), and () and to nematodes (Nematoda). In some embodiments, expression of one or more protein toxins (e.g., insecticidal proteins) in the auxinic-herbicide tolerant plants is effective for controlling organisms that include, for example, members of the classes and orders: Coleoptera such as the American bean weevil Acanthoscelides obtectus; the leaf beetle Agelastica alni; click beetles (Agriotes lineatus, Agriotes obscurus, Agriotes bicolor); the grain beetle Ahasverus advena; the summer schafer Amphimallon solstitialis; the furniture beetle Anobium punctatum; Anthonomus spp. (weevils); the Pygmy mangold beetle Atomaria linearis; carpet beetles (Anthrenus spp., Attagenus spp.); the cowpea weevil Callosobruchus maculates; the fried fruit beetle Carpophilus hemipterus; the cabbage seedpod weevil Ceutorhynchus assimilis; the rape winter stem weevil Ceutorhynchus picitarsis; the wireworms Conoderus vespertinus and Conoderus falli; the banana weevil Cosmopolites sordidus; the New Zealand grass grub Costelytra zealandica; the June beetle Cotinis nitida; the sunflower stem weevil Cylindrocopturus adspersus; the larder beetle Dermestes lardarius; the corn rootworms Diabrotica virgifera, Diabrotica virgifera virgifera, and Diabrotica barberi; the Mexican bean beetle Epilachna varivestis; the old house borer Hylotropes bajulus; the lucerne weevil Hypera postica; the shiny spider beetle Gibbium psylloides; the cigarette beetle Lasioderma serricorne; the Colorado potato beetle Leptinotarsa decemlineata; Lyctus beetles (Lyctus spp.); the pollen beetle Meligethes aeneus; the common cockshafer Melolontha melolontha; the American spider beetle Mezium americanum; the golden spider beetle Niptus hololeucus; the grain beetles Oryzaephilus surinamensis and Oryzaephilus Mercator; the black vine weevil Otiorhynchus sulcatus; the mustard beetle Phaedon cochleariae, the crucifer flea beetle Phyllotreta cruciferae; the striped flea beetle Phyllotreta striolata; the cabbage steam flea beetle Psylliodes chrysocephala; Ptinus spp. (spider beetles); the lesser grain borer Rhizopertha dominica; the pea and been weevil Sitona lineatus; the rice and granary beetles Sitophilus oryzae and Sitophilus granaries; the red sunflower seed weevil Smicronyx fulvus; the drugstore beetle Stegobium paniceum; the yellow mealworm beetle Tenebrio molitor; the flour beetles Tribolium castaneum and Tribolium confusum; warehouse and cabinet beetles (Trogoderma spp.); the sunflower beetle Zygogramma exclamationis; Dermaptera (earwigs) such as the European earwig Forficula auricularia and the striped earwig Labidura riparia; Dictyoptera such as the oriental cockroach Blatta orientalis; the greenhouse millipede Oxidus gracilis; the beet fly Pegomyia betae; the frit fly Oscinellafrit; fruitfiies (Dacus spp. , Drosophila spp.); Isoptera (termites) including species from the families Hodotermitidae, Kalotermitidae, Mastotermitidae, Rhinotermitidae, Serritermitidae, Termitidae, Termopsidae; the tarnished plant bug Lygus lineolaris; the black bean aphid Aphis fabae; the cotton or melon aphid Aphis gossypii; the green apple aphid Aphis pomi; the citrus spiny whitefly Aleurocanthus spiniferus; the sweet potato whitefly Bemesia tabaci; the cabbage aphid Brevicoryne brassicae; the pear psylla Cacopsylla pyricola; the currant aphid Cryptomyzus ribis; the grape phylloxera Daktulosphaira vitifoliae; the citrus psylla Diaphorina citri; the potato leafhopper Empoasca fabae; the bean leafhopper Empoasca Solana; the vine leafhopper Empoasca vitis; the woolly aphid Eriosoma lanigerum; the European fruit scale Eulecanium corni; the mealy plum aphid Hyalopterus arundinis; the small brown planthopper Laodelphax striatellus; the potato aphid Macrosiphum euphorbiae; the green peach aphid Myzus persicae; the green rice leafhopper Nephotettix cinticeps; the brown planthopper Nilaparvata lugens; the hop aphid Phorodon humuli; the bird-cherry aphid Rhopalosiphum padi; the grain aphid Sitobion avenae; Lepidoptera such as Adoxophyes orana (summer fruit tortrix ); Archips podana (fruit tree tortrix moth); Bucculatrix pyrivorella (pear leafminer); Bucculatrix thurberiella (cotton leaf perforator); Bupalus piniarius (pine looper); Carpocapsa pomonella (codling moth); Chilo suppressalis (striped rice borer); Choristoneura fumiferana (eastern spruce budworm); Cochylis hospes (banded sunflower moth); Diatraea grandiosella (southwestern corn borer); Eupoecilia ambiguella (European grape berry moth); Helicoverpa armigera (cotton bollworm); Helicoverpa zea (cotton bollworm); Heliothis virescens (tobacco budworm), Homeosoma electellum (sunflower moth); Homona magnanima (oriental tea tree tortrix moth); Lithocolletis blancardella (spotted tentiform leafminer); Lymantria dispar (gypsy moth); Malacosoma neustria (tent caterpillar); Mamestra brassicae (cabbage armyworm); Mamestra configurata (Bertha armyworm); Operophtera brumata (winter moth); Ostrinia nubilalis (European corn borer), Panolis flammea (pine beauty moth), Phyllocnistis citrella (citrus leafminer); Pieris brassicae (cabbage white butterfly); Rachiplusia ni ( looper); Spodoptera exigua (beet armyworm); Spodoptera littoralis (cotton leafworm); Sylepta derogata (cotton leaf roller); Trichoplusia ni (cabbage looper); Orthoptera such as the common cricket Acheta domesticus, tree locusts (Anacridium spp.), the migratory locust Locusta migratoria, the two striped grasshopper Melanoplus bivittatus, the differential grasshopper Melanoplus differentialis, the red legged grasshopper Melanoplus femurrubrum, the migratory grasshopper Melanoplus sanguinipes, the northern mole cricket Neocurtilla hexadectyla, the red locust Nomadacris septemfasciata, the shortwinged mole cricket Scapteriscus abbreviatus, the southern mole cricket Scapteriscus borellii, the tawny mole cricket Scapteriscus vicinus, and the desert locust Schistocerca gregaria; Symphyla such as the garden symphylan Scutigerella immaculata; Thysanoptera such as the tobacco thrips Frankliniella fusca, the flower thrips Frankliniella intonsa, the western flower thrips Frankliniella occidentalis, the cotton bud thrips Frankliniella schultzei, the banded greenhouse thrips Hercinothrips femoralis, the soybean thrips Neohydatothrips variabilis, Kelly's citrus thrips Pezothrips kellyanus, the avocado thrips Scirtothrips perseae, the melon thrips Thrips palmi, and the onion thrips Thrips tabaci; and the like, and combinations comprising one or more of the foregoing organisms. In some embodiments, expression of one or more protein toxins (e.g., insecticidal proteins) in the auxinic-herbicide tolerant plants is effective for controlling flea beetles, i.e., members of the flea beetle tribe of family Chrysomelidae, preferably against Phyllotreta spp., such as Phyllotreta cruciferae and/or Phyllotreta triolata. In other embodiments, expression of one or more protein toxins (e.g., insecticidal proteins) in the auxinic-herbicide tolerant plants is effective for controlling cabbage seedpod weevil, the Bertha armyworm, Lygus bugs, or the diamondback moth. Furthermore, in one embodiment, auxinic herbicide-tolerant plants (e.g., oilseed rape (canola)) are also covered which are, e.g., by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such trait, rendered able to synthesize one or more proteins to increase the resistance or tolerance of those plants to bacterial, viral or fungal pathogens. The methods for producing such genetically modified plants are generally known to the person skilled in the art. Furthermore, in another embodiment, auxinic herbicide-tolerant plants (e.g., oilseed rape (canola)) are also covered which are, e.g., by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such trait, rendered able to synthesize one or more proteins to increase the productivity (e.g., oil content), tolerance to drought, salinity or other growth-limiting environmental factors or tolerance to pests and fungal, bacterial or viral pathogens of those plants. Furthermore, in other embodiments, auxinic herbicide-tolerant plants (e.g., oilseed rape (canola)) are also covered which are, e.g., by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such trait, altered to contain a modified amount of one or more substances or new substances, for example, to improve human or nutrition, e.g., oil crops that produce health-promoting long-chain omega-3 fatty acids or unsaturated omega-9 fatty acids (e.g., Nexera ® rape, Dow Agro Sciences, Canada). Furthermore, in some embodiments, auxinic herbicide-tolerant plants are also covered which are, e.g., by the use of recombinant DNA techniques and/or by breeding and/or otherwise selected for such trait, altered to contain increased amounts of vitamins and/or minerals, and/or improved profiles of nutraceutical compounds. In one embodiment, auxinic herbicide-tolerant plants described herein, relative to a wild- type plant, comprise an increased amount of, or an improved profile of, a compound selected from the group consisting of: glucosinolates (e.g., glucoraphanin (4-methylsulfmylbutyl- glucosinolate), sulforaphane, 3-indolylmethyl-glucosinolate (glucobrassicin), l-methoxy-3- indolylmethyl-glucosinolate (neoglucobrassicin)); phenolics (e.g., flavonoids (e.g., quercetin, kaempferol), hydroxycinnamoyl derivatives (e.g., l,2,2'-trisinapoylgentiobiose, 1,2- diferuloylgentiobiose, 1,2'-disinapoyl-2-feruloylgentiobiose, 3-O-caffeoyl-quinic (neochlorogenic acid)); and vitamins and minerals (e.g., vitamin C, vitamin E, carotene, folic acid, niacin, riboflavin, thiamine, calcium, iron, magnesium, potassium, selenium, and zinc). In another embodiment, auxinic herbicide-tolerant plants described herein, relative to a wild-type plant, comprise an increased amount of, or an improved profile of, a compound selected from the group consisting of: progoitrin; isothiocyanates; indoles (products of glucosinolate hydrolysis); glutathione; carotenoids such as beta-carotene, lycopene, and the xanthophyll carotenoids such as lutein and zeaxanthin; phenolics comprising the flavonoids such as the flavonols (e.g., quercetin, rutin), the flavans/tannins (such as the procyanidins comprising coumarin, proanthocyanidins, catechins, and anthocyanins); flavones; phytoestrogens such as coumestans, lignans, resveratrol, isoflavones e.g., genistein, daidzein, and glycitein; resorcyclic acid lactones; organosulphur compounds; phytosterols; terpenoids such as carnosol, rosmarinic acid, glycyrrhizin and saponins; chlorophyll; chlorophyllin, sugars, anthocyanins, and vanilla. In other embodiments, auxinic herbicide-tolerant plants described herein, relative to a wild-type plant, comprise an increased amount of, or an improved profile of, a compound selected from the group consisting of: vincristine, vinblastine, taxanes (e.g., taxol (paclitaxel), baccatin III, 10-desacetylbaccatin III, 10-desacetyl taxol, xylosyl taxol, 7-epitaxol, 7-epibaccatin III, 10-desacetylcephalomannine, 7-epicephalomannine, taxotere, cephalomannine, xylosyl cephalomannine, taxagifme, 8-benxoyloxy taxagifme, 9-acetyloxy taxusin, 9-hydroxy taxusin, taiwanxam, taxane la, taxane lb, taxane Ic, taxane Id, GMP paclitaxel, 9-dihydro 13- acetylbaccatin III, 10-desacetyl-7-epitaxol, tetrahydrocannabinol (THC), cannabidiol (CBD), genistein, diadzein, codeine, morphine, quinine, shikonin, ajmalicine, serpentine, and the like. In one embodiment, auxinic herbicide-tolerant plants described herein also can be tolerant to herbicides that inhibit acetohydroxyacid synthase (AHAS). As used herein, "herbicide tolerant AHASL" refers to the AHAS large subunit polypeptide expressed from one mutant AHASL allele of an AHASL gene in a plant cell and/or from either or both of two homologous alleles of the same mutant AHASL gene, i.e., in the same genome of, the plant cell, whereby that mutant AHASL can provide herbicide tolerance to an AHAS enzyme of the plant cell. A mutant AHASL gene can be recombinant, or can be obtained by application of a mutagenesis process, a breeding process, or other process known in the art. Such a gene can be hemizygous, heterozygous, or homozygous. As used herein, "AHAS" and "AHASL" respectively refer to functional, plastidic AHAS enzymes and AHASL polypeptides thereof, i.e., which are functional in cells of the plants as described herein. Similarly, terms such as "gene" and "polynucleotide," when used in reference to those encoding such an "AHAS" and "AHASL," refer to functional genes therefor, i.e., genes that are expressible in such a cell. As used herein, standard one-letter abbreviations for amino acids will be used, for example, A indicates alanine, P indicates proline, W indicates tryptophan, X indicates any amino acid, etc. Mutations as compared to the wild-type sequence will be indicated by specifying the wild-type amino acid and position followed by the amino acid present in the mutant. For example, P197X will be used to indicate that the proline at position 197 can be substituted with any amino acid. For ease of understanding, when referring to amino acid positions in an AHASL, the amino acid numbering system used herein may be the industry standard numbering used for the Arabidopsis thaliana {At) AHASL sequence, and can be denoted with an {At). For example, P197( t) can refer to the proline residue at the position in a Brassica AHASL that corresponds to the proline at position 197 of the Arabidopsis thaliana AHASL. As used herein, an AHAS herbicide-tolerance-inducing mutation is an alteration in the amino acid sequence of an AHASL enzyme that confers tolerance to one or more herbicides (i.e., sulfonylurea herbicides, imidazolinone herbicides, etc.). The following Table 1 provides a non-limiting list of possible sites for AHASL mutations, permissible substitutions, preferred substitutions, and more preferred substitutions. X indicates any amino acid.

In some embodiments, AHASL mutations can be selected from the group consisting of A122X, P197X, R199X, A205X, S653X, and G654X, and combinations thereof. In other embodiments, AHASL mutations can be selected from the group consisting of A122T, A122V, A122D, A122P, A122Y, P197S, P197L, P197T, R199A, R199E, A205V, A205C, A205D, A205E, A205R, A205T, A205W, A205Y, A205N, S653N, S653I, S653F, S653T, G654Q, G654C, W574L, W574M, W574C, W574S, W574R, W574G, W574A, W574F, W574Q, W574Y, G654E, G654D, and combinations thereof. In some embodiments, AHASL mutations can be selected from the group consisting of A122T, A122V, R199A, R199E, A205V, S653N, G654E, and combinations thereof. Sources of useful plastidic AHASL genes can be provided from any of the following deposited cell lines listed in Table 2, of Brassica napus (Bn) and Brassicajuncea (Bj), wherein their AHAS-inhibitor-tolerant AHAS large subunit (AHASL) alleles are referred to as shown below, with the final letter indicating the Brassica genome (A, B, or C) to which the allele is native: BnAHASLlA or BnAHASLIC for B. napus, and BjAHASLlA or BjAHASLIB for B. juncea. Note that AHASL mutation positions are stated with reference to the standardized nomenclature in the field, in which the Arabidopsis thaliana {At) plastidic AHASL polypeptide provides the standard for residue position numbering.

Patents documents referred to in Table 2 are herein incorporated by reference entirety. As is WO 2009/046334 to Schopke et al. In various embodiments, auxinic herbicide-tolerant plants, e.g., Brassica plants, may further comprise an AHASL containing both a W574(^t )X and a S653(^t )X in plastidic AHASL polypeptides. These AHASL mutations can be present in different alleles, such as on different genomes, with each containing a single mutation in the respective AHASL gene, or these two can be present in a single AHASL, as double-mutant allele. In various embodiments, these can be W574(^t )L and S653(^t )N: the former can be referred to as the "PM2" mutation and the latter as the "PM1" mutation. In some embodiments, the auxinic herbicide-tolerant plants hereof can be inbred varieties, e.g., open-pollinated varieties, or hybrids, e.g., Fl hybrids. In other embodiments, the auxinic herbicide-tolerant plants further comprise one or more mutant AHASL genes that may be transgenic or non-transgenic mutant AHAS genes. In various embodiments, the AHAS trait can be non-transgenic, i.e., obtained by a process, excluding recombinant DNA techniques, and comprising mutagenesis, genoplasty, and/or isolation of spontaneous mutant plants. Many mutagenesis techniques are known in the art and these can involve application of a mutagenic chemical agent or radiation to seeds, plants parts, or cultured plant cells; alternatively, or in addition, the culturing of plant cells, or the conditions under which plant cells are cultured, can increase the rate of occurrence or accumulation of spontaneous mutations. Genoplasty techniques can include directed mutation-type strategies, such as methods comprising introduction, into the plant cell nucleus, of oligonucleotides that facilitate mismatch-repair-system-mediated nucleotide substitution. Other aspects described herein are auxinic herbicide-tolerant plants further comprising tolerance to at least one ACCase inhibitor herbicide at levels that would normally inhibit the growth of wild-type plant. In some embodiments, the auxinic herbicide-tolerant plant described herein expresses an ACCase in which the amino acid sequence differs from an amino acid sequence of an ACCase of a wild-type plant. For ease of understanding, when referring to amino acid positions in an ACCase, the amino acid numbering system used herein may be the industry standard numbering system used for the ACCase from Alopecurus myosuroides [Huds.] (also referred to as black grass). The mR A (cDNA) sequence encoding the A. myosuroides ACCase is available at GenBank accession number AJ3 10767 and the protein sequence is available at GenBank accession No. CAC84161 both of which are specifically incorporated herein by reference. The number of the amino acid referred to will be followed with (Am) to indicate the amino acid in the Alopecurus myosuroides sequence to which the amino acid corresponds. Table 3 shows the Alopecurus myosuroides ACCase amino acid sequence GenBank accession No. CAC84161. Amino acids that can be altered in the ACCase enzymes are indicted in bold double underline. Table 3. Alopecurus myosuroides ACCase amino acid sequence (SEQ ID NO:7) (GenBank accession No. CAC84161). 1 MGSTHLPIVG FNASTTPSLS TLRQINSAAA AFQSSSPSRS SKKKSRRVKS IRDDGDGSVP 6 1 DPAGHGQSIR QGLAGI IDLP KEGASAPDVD ISHGSEDHKA SYQMNGILNE SHNGRHASLS 121 KVYEFCTELG GKTPIHSVLV ANNGMAAAKF MRSVRTWAND TFGSEKAIQL IAMATPEDMR 181 INAEHIRIAD QFVEVPGGTN NNNYANVQLI VEIAERTGVS AV PG GHAS ENPELPDALT 241 AKGIVFLGPP ASSMNALGDK VGSALIAQAA GVPTLAWSGS HVEIPLELCL DSIPEEMYRK 301 ACVTTADEAV ASCQMIGYPA MIKASWGGGG KGIRKVNNDD EVKALFKQVQ GEVPGSPIFI 361 MRLASQSRHL EVQLLCDEYG NVAALHSRDC SVQRRHQKI EEGPVTVAPR ETVKELEQAA 421 RRLAKAVGYV GAATVEYLYS METGEYYFLE LNPRLQVEHP VTESIAEVNL PAAQVAVGMG 481 IPLWQIPEIR RFYGMDNGGG YDIWRKTAAL ATPFNFDEVD SQWPKGHCVA VRITSENPDD 541 GFKPTGGKVK EISFKSKPNV WGYFSVKSGG GIHEFADSQF GHVFAYGETR SAAITSMSLA 601 LKEIQIRGEI HTNVDYTVDL LNAPDFRENT IHTGWLDTRI AMRVQAERPP WYISWGGAL 661 YKTITTNAET VSEYVSYLIK GQIPPKHISL VHSTISLNIE ESKYTIEIVR SGQGSYRLRL 721 NGSLIEANVQ TLCDGGLLMQ LDGNSHVIYA EEEAGGTRLL IDGKTCLLQN DHDPSRLLAE 781 TPCKLLRFLI ADGAHVDADV PYAEVEVMKM CMPLLSPAAG VINVLLSEGQ AMQAGDLIAR 841 LDLDDPSAVK RAEPFEGSFP EMSLPIAASG QVHKRCAASL NAARMVLAGY DHAANKWQD 901 LVWCLDTPAL PFLQWEELMS VLATRLPRRL KSELEGKYNE YKLNVDHVKI KDFPTEMLRE 961 TIEENLACVS EKEMVTIERL VDPLMSLLKS YEGGRESHAH FIVKSLFEEY LSVEELFSDG 1021 IQSDVIERLR LQYSKDLQKV VDIVLSHQGV RNKTKLILAL MEKLVYPNPA AYRDQLIRFS 1081 SLNHKRYYKL ALKASELLEQ TKLSELRTSI ARNLSALDMF TEEKADFSLQ DRKLAINESM 1141 GDLVTAPLPV EDALVSLFDC TDQTLQQRVI QTYISRLYQP QLVKDSIQLK YQDSGVIALW 1201 EFTEGNHEKR LGAMVILKSL ESVSTAIGAA LKDASHYASS AGNTVHIALL DADTQLNTTE 12 6 1 DSGDNDQAQD KMDKLSFVLK QDWMADLRA ADVKWSCIV QRDGAIMPMR RTFLLSEEKL 1321 CYEEEPILRH VEPPLSALLE LDKLKVKGYN EMKYTPSRDR QWHIYTLRNT ENPKMLHRVF 1381 FRTLVRQPSA GNRFTSDHIT DVEVGHAEEP LSFTSSSILK SLKIAKEELE LHAIRTGHSH 1441 MYLCILKEQK LLDLVPVSGN TWDVGQDEA TACSLLKEMA LKIHELVGAR MHHLSVCQWE 1501 VKLKLVSDGP ASGSWRWTT NVTGHTCTVD IYREVEDTES QKLVYHSTAL SSGPLHGVAL 1561 NTSYQPLSVI DLKRCSARNN KTTYCYDFPL TFEAAVQKSW SNISSENNQC YVKATELVFA 1621 EKNGSWGTPI IPMQRAAGLN DIGMVAWILD MSTPEFPSGR QIIVIANDIT FRAGSFGPRE 1681 DAFFEAVTNL ACEKKLPLIY LAANSGARIG IADEVKSCFR VGWTDDSSPE RGFRYIYMTD 1741 EDHDRIGSSV IAHKMQLDSG EIRWVIDSW GKEDGLGVEN HGS IASA YSRAYEETFT 1801 LTFVTGRTVG IGAYLARLGI RCIQRIDQPI ILTGFSALNK LLGREVYSSH MQLGGPKIMA 1861 TNGYVHLTVP DDLEGVSNIL RWLSYVPANI GGPLPITKSL DPIDRPVAYI PENTCDPRAA 1921 ISGIDDSQGK WLGGMFDKDS FVETFEGWAK TWTGRAKLG GIPVGVIAVE TQTMMQLVPA 1981 DPGQPDSHER SVPRAGQV^F PDSATKTAQA MLDFNREGLP LFILAN^RGF SGGQRDLFgG 2041 ILQAGSTIYE NLRTYNQP^F VYIPKAAELR GGAWWI DSK INPDRIEgYA ERTAKGNVLE 2101 PQGLIEIKFR SEELKECMGR LDPELIDLKA RLQGANGSLS DGESLQKSIE ARKKQLLPLY 2161 TQIAVRFAEL HDTSLRMAAK GVIRKWDWE DSRSFFYKRL RRRLSEDVLA KEIRGVIGEK 2221 FPHKSAIELI KKWYLASEAA AAGSTDWDDD DAFVAWRENP ENYKEYIKEL RAQRVSRLLS 2281 DVAGSSSDLQ ALPQGLSMLL DKMDPSKRAQ FIEEVMKVLK

Examples of amino acid positions at which an ACCase of an auxininc herbicide-tolerant plant differs from the ACCase of the corresponding wild-type plant include, but are not limited to, one or more of the following positions: \,l%\(Am), \,l%5(Am), \,l%6(Am), \ , \ \(Am), \,%24(Am), \ 4(Am) , 1,999(Am), Ifill(Am), 2,039(Am), 2,041 (Am), 2,049(Am), 2,059(Am), 2,014(Am), 2,015(Am), 2,07S(Am), 2,019(Am), 2,080(Am), 2,0Sl(Am), 2,0SS(Am), 2,095(Am), 2,096(Am), o 2,098(Am). In other aspects, a method for treating a plant described herein is provided. In some embodiments, the method comprises contacting the plant with an agronomically acceptable composition. In one embodiment, the agronomically acceptable composition comprises an auxinic herbicide A.I. Another aspect described herein is a method for preparing a descendent seed. The method comprises planting a seed of or capable of producing a plant described herein. For example, one embodiment described herein is a method for preparing a descendent seed, the method comprising planting a seed of or capable of producing an auxinic herbicide tolerant plant or plant part thereof having the auxinic herbicide-tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, with the proviso that the plant is a monocot or dicot species other than Sinapis arvensis. In another embodiment, the method further comprises growing a descendent plant from the seed and harvesting a descendant seed from the descendent plant. In other embodiments, the method further comprises applying an auxinic herbicidal composition to the descendent plant. A further aspect described herein is a method for producing a plant product. In some embodiments, the method comprises processing a plant or plant part thereof described herein. For example, one embodiment described herein is a method for producing a plant product, the method comprising processing an auxinic herbicide tolerant plant or plant part thereof having the auxinic herbicide-tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, with the proviso that the plant is a monocot or dicot species other than Sinapis arvensis, to obtain the plant product therefrom. In some embodiments, the plant product is fodder, seed meal, oil, or seed-treatment-coated seeds. In other embodiments, the plant part is a seed. Herbicides In some embodiments, herbicide compositions described herein comprise one or more auxinic herbicides that can be chosen from among the compounds listed in Table 4 and their agronomically acceptable salts and esters. As used herein "auxinic herbicides" include these compounds and such salts, esters, or derivatives thereof. In some embodiments, the l dose rates for auxinic herbicide A.I.s useful herein are shown below in Table 5; these are also applicable to the salt or ester forms thereof.

In some embodiments, auxinic herbicide tolerant plants described herein comprise tolerance to application of auxinic herbicide in an amount of about 5 to about 5000 g/ha A.I., illustratively, about 10 to about 5000, about 50 to about 4950, about 100 to about 4900, about 150 to about 4850, about 200 to about 4800, about 250 to about 4750, about 300 to about 4700, about 350 to about 4650, about 400 to about 4600, about 450 to about 4550, about 500 to about 4500, about 550 to about 4450, about 600 to about 4400, about 650 to about 3500, about 700 to about 3450, about 750 to about 3400, about 800 to about 3350, about 850 to about 3300, about 900 to about 3250, about 950 to about 3200, about 1000 to about 3150, about 1050 to about 3100, about 1100 to about 3050, about 1150 to about 3000, about 1200 to about 2950, about 1250 to about 2900, about 1300 to about 2850, about 1350 to about 2800, about 1400 to about 2750, about 1450 to about 2700, about 1500 to about 2650, about 1550 to about 2600, about 1600 to about 2550, about 1650 to about 2500, about 1700 to about 2450, about 1750 to about 2400, about 1800 to about 2350, about 1850 to about 2300, about 1900 to about 2250, about 1950 to about 2200, about 2000 to about 2150, and about 2050 to about 2100 g/ha A.I. In one embodiment, in either pre-emergent or post-emergent weed control methods hereof, the method can utilize 1 auxinic herbicide application rates with no significant injury to the plant; in some embodiments thereof, the application rate can exceed 1 auxinic herbicide; in some embodiments, the rate can be up to 4 auxinic herbicide, though more typically it will be about 2.5 or less, or about 2x or less. Where a combination of these auxinic herbicide A.I.s is employed, the herbicide application rate will preferably provide a summed rate that falls within at least about 0.25 x , illustratively, about 0.25 x to about 4x, about 0.5 X to about 3X, about 1 to about 2x auxinic herbicide range. In one embodiment, the auxinic herbicide composition comprises at least one of clomeprop; cloprop ("3-CPA"); 4-chlorophenoxyacetic acid ("4-CPA"); 2-(4- chlorophenoxy)propionic acid ("4-CPP"); 2,4-dichlorophenoxy acetic acid ("2,4-D"); (3,4- dichlorophenoxy)acetic acid ("3,4-DA"); 4-(2,4-dichlorophenoxy)butyric acid ("2,4-DB"); 2- (3,4-dichlorophenoxy)propionic acid ("3,4-DP"); tris[2-(2,4-dichlorophenoxy)ethyl]phosphite ("2,4-DEP"); ("2,4-DP"); 2,4,5-trichlorophenoxyacetic acid ("2,4,5-T"); ("2,4,5-TP"); 2-(4-chloro-2-methylphenoxy)acetic acid ("MCPA"); 4-(4-chloro-2- methylphenoxy)butyric acid ("MCPB"); ("MCPP"); ; dicamba; tricamba; 2,3,6-trichlorobenzoic acid ("TBA"); ; ; ; ; ; ; quinmerac; or benazolin. In another embodiment, the auxinic herbicide composition comprises at least one of clomeprop; cloprop ("3-CPA"); 4-chlorophenoxyacetic acid ("4-CPA"); 2-(4- chlorophenoxy)propionic acid ("4-CPP"); (3,4-dichlorophenoxy)acetic acid ("3,4-DA"); 4-(2,4- dichlorophenoxy)butyric acid ("2,4-DB"); 2-(3,4-dichlorophenoxy)propionic acid ("3,4-DP"); tris[2-(2,4-dichlorophenoxy)ethyl]phosphite ("2,4-DEP"); dichlorprop ("2,4-DP"); 2,4,5- trichlorophenoxyacetic acid ("2,4,5-T"); fenoprop ("2,4,5-TP"); 4-(4-chloro-2- methylphenoxy)butyric acid ("MCPB"); chloramben; dicamba; tricamba; 2,3,6-trichlorobenzoic acid ("TBA"); aminopyralid; clopyralid; picloram; quinclorac; quinmerac; or benazolin. In another embodiment, the auxinic herbicide composition comprises at least one of: 4- (2,4-dichlorophenoxy)butyric acid ("2,4-DB"); dicamba; aminopyralid; picloram; or quinclorac. In another embodiment, the auxinic herbicide composition comprises at least one of: aminopyralid or picloram. In another embodiment, the auxinic herbicide composition comprises dicamba. In other embodiments, in addition to the one or more auxinic herbicides, the herbicide compositions described herein, optionally, can further comprise one or more agronomically acceptable A.I.(s), e.g., herbicidal A.I.s. As used herein, agronomically acceptable A.I.(s) include the A.I.s and their agronomically acceptable salts and esters. Additional classes of herbicides include, but are not limited to, AHAS inhibitors; bleaching herbicides such as hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors or phytoene desaturase (PDS) inhibitors; enolpyruvyl shikimate 3-phosphate synthase (EPSPS) inhibitors such as glyphosate; glutamine synthetase (GS) inhibitors such as glufosinate; protoporphyrinogen-IX oxidase (PPO) inhibitors; lipid biosynthesis inhibitors such as ACCase inhibitors; or oxynil (i.e., bromoxynil or ioxynil) herbicides. AHAS-inhibitor herbicides include, e.g., imidazolinone herbicides, one or more SU herbicides selected from the group consisting of amidosulfuron, flupyrsulfuron, foramsulfuron, imazosulfuron, iodosulfuron, mesosulfuron, nicosulfuron, thifensulfuron, and tribenuron, agronomically acceptable salts and esters thereof, and combinations thereof. ACCase inhibitor herbicides include, e.g., "dims" (e.g., cycloxydim, sethoxydim, clethodim, or tepraloxydim), "fops" (e.g., clodinafop, diclofop, fluazifop, haloxyfop, or quizalofop), and "dens" (such as pinoxaden). In addition to the one or more auxinic herbicides, the herbicide compositions described herein, optionally, can further comprise one or more agronomically acceptable A.I.(s) of other classes, e.g., agronomic fungicides, bactericides, algicides, nematicides, insecticides, and the like (e.g., malathion, pyrethrins/pyrethrum, carbaryl, spinosad, permethrin, bifenthrin, and esfenvalerate). The herbicidal compositions hereof comprising one or more auxinic herbicides, and optionally other agronomic A.I.(s) can be used in any agronomically acceptable format. For example, these can be formulated as ready-to-spray aqueous solutions, powders, suspensions; as concentrated or highly concentrated aqueous, oily, or other solutions, suspensions, or dispersions; as emulsions, oil dispersions, pastes, dusts, granules, or other broadcastable formats. The herbicide compositions can be applied by any means known in the art, including, for example, spraying, atomizing, dusting, spreading, watering, seed treatment, or co-planting in admixture with the seed. The use forms depend on the intended purpose; in any case, they should ensure the finest possible distribution of the A.I.s described herein. In some embodiments, an herbicidal composition hereof can comprise, e.g., a combination of: auxinic herbicide(s), e.g., dicamba; AHAS-inhibitor(s), e.g., imidazolinone(s) and/or sulfonylurea(s); ACCase-inhibitor(s); EPSPS inhibitor(s), e.g., glyphosate; glutamine synthetase inhibitor(s), e.g.., glufosinate; protoporphyrinogen-IX oxidase (PPO) inhibitor(s), e.g., saflufenacil; fungicide(s), e.g., strobilurin fungicide(s) such as pyraclostrobin; and the like. In some embodiments, an herbicidal composition hereof can comprise, e.g., a combination of auxinic herbicide(s), e.g., dicamba; and strobilurin fungicide(s) such as pyraclostrobin(s). An herbicidal composition will be selected according to the tolerances of a plant hereof, and the plant can be selected from among those having stacked tolerance traits. In some embodiments, where the optional A.I. includes an AHAS-inhibitor, this can be selected from: (1) the imidazolinones, e.g., imazamox, imazethapyr, , , imazaquin, and imazamethabenz, preferably from imazamox, imazethapyr, imazapyr, and imazapic, preferably imazamox; (2) the sulfonylureas, e.g., amidosulfuron, flupyrsulfuron, foramsulfuron, imazosulfuron, iodosulfuron, mesosulfuron, nicosulfuron, thifensulfuron, and tribenuron; (2) the pyrimidinyloxy[thio]benzoates, e.g., including the pyrimidinyloxybenzoates (e.g., bispyribac, pyriminobac, and ) and the pyrimidinylthiobenzoates (e.g., pyrithiobac and pyriftalid); and (3) the sulfonamides, i.e., including the sulfonylaminocarbonyltriazolinones (e.g., flucarbazone and propoxycarbazone) and the triazolopyrimidines (e.g., cloransulam, diclosulam, florasulam, flumetsulam, metosulam, and penoxsulam). The agronomically acceptable salts and esters of the foregoing are also included, as are combinations thereof. In other embodiments, where the optional A.I. includes an herbicide from a different class to which the plant(s) hereof would normally be susceptible, the plant to be used is selected from among those that further comprise a trait of tolerance to such herbicide. Such further tolerance traits can be provided to the plant by any method known in the art, e.g., including techniques of traditional breeding to obtain a tolerance trait gene by hybridization or introgression, of mutagenesis, and/or of transformation. Such plants can be described as having "stacked" traits. Optional A.I.s of other herbicide classes include: ACCase inhibitors, PPO inhibitors, EPSPS inhibitors, glutamine synthetase inhibitors, p-hydroxyphenylpyruvate dioxygenase (4- HPPD) inhibitors. Optional A.I.s of other types include, but are not limited to fungicides such as strobilurins, e.g., pyraclostrobin, alone or in combination with, e.g., boscalid, epiconazole, metaconazole, tebuconazole, kresoxim-methyl, fluxapyroxad, isopyrazam, flutolanil, and the like; insecticides such as lepidoptericides, coleoptericides, and the like; nematicides; molluskicides; and others known in the art. In some embodiments, optional A.I.s include fungicides such as strobilurins (e.g., pyraclostrobin), boscalid, epiconazole, metaconazole, tebuconazole, kresoxim-methyl, fluxapyroxad, isopyrazam, flutolanil, and the like, each alone or in combination. For example, suitable examples of herbicides that are ACCase inhibitors include, but are not limited to, cyclohexanedione herbicides (DIMs, also referred to as: cyclohexene oxime cyclohexanedione oxime; and CHD), aryloxyphenoxy propionate herbicides (also referred to as aryloxyphenoxy propanoate; aryloxyphenoxyalkanoate; oxyphenoxy; APP; AOPP; APA; APPA; FOP, note that these are sometime written with the suffix '-oic'), and phenylpyrazole herbicides (also known as DENs; and sometimes referred to under the more general class of Phenylpyrazole such as pinoxaden (e.g., herbicides sold under the trade names Axial and Traxos). In some methods of controlling weeds and/or growing herbicide-tolerant plants, at least one herbicide is selected from the group consisting of sethoxydim, cycloxydim, tepraloxydim, haloxyfop, haloxyfop-P or a derivative of any of these herbicides. Table 6 lists herbicides that interfere with ACCase activity. Table 6. Examples of ACCase inhibitors. ACCase Inhibitor Class Company Examples of Synonyms and Trade Names Fervin, Kusagard, NP-48Na, BAS 90 1H, alloxydim DIM BASF Carbodimedon, Zizalon butroxydim DIM Syngenta Falcon, ICI-A0500, Butroxydim clethodim DIM Valent Select, Prism, Centurion, RE-45601, Motsa clodinafop-propargyl FOP Syngenta Discover, Topik, CGA 184 927 clofop FOP Alopex cloproxydim FOP chlorazifop FOP cycloxydim DIM BASF Focus, Laser, Stratos, BAS 517H cyhalofop-butyl FOP Dow Clincher, XDE 537, DEH 112, Barnstorm Hoegrass, Hoelon, Illoxan, HOE 23408, Dichlorfop, diclofop-methyl FOP Bayer Illoxan Super Whip, Option Super, Exel Super, HOE-46360, fenoxaprop-P-ethyl FOP Bayer Aclaim, Puma S, Fusion fenthiaprop FOP Taifun; Joker Fusilade, Fusilade 2000, Fusilade DX, ICI-A 0009, fluazifop-P-butyl FOP Syngenta ICI-A 0005, SL-236, IH-773B, TF-1 169, Fusion haloxyfop-etotyl FOP Dow Gallant, DOWCO 453EE haloxyfop-methyl FOP Dow Verdict, DOWCO 453ME haloxyfop-P-methyl FOP Dow Edge, DE 535 isoxapyrifop FOP metamifop FOP Dongbu NA pinoxaden DEN Syngenta Axial profoxydim DIM BASF Aura, Tetris, BAS 625H, Clefoxydim propaquizafop FOP Syngenta Agil, Shogun, Ro 17-3664, Correct Assure, Assure II, DPX-Y6202-3, Targa Super, NC- quizalofop-P-ethyl FOP DuPont 302, Quizafop quizalofop-P-tefuryl FOP Uniroyal Pantera, UBI C4874 Poast, Poast Plus, NABU, Fervinal, NP-55, Sertin, Sethoxydim DIM BASF BAS 562H, Cyethoxydim, Rezult tepraloxydim DIM BASF BAS 620H, Aramo, Caloxydim Achieve, Splendor, ICI-A0604, Tralkoxydime, tralkoxydim DIM Syngenta Tralkoxidym trifop FOP

The herbicidal compositions comprising an auxinic herbicide, and optionally other agronomic A.I.(s) and/or their agriculturally suitable salts and esters can also comprise auxiliaries, which are customary for the formulation of crop protection agents. Examples of auxiliaries customary for the formulation of crop protection agents include inert auxiliaries, solid carriers, surfactants (such as dispersants, protective colloids, emulsifiers, wetting agents and tackifiers), organic and inorganic thickeners, penetrants (such as penentration-enhancing organosilicone surfactants or acidic sulfate chelates, e.g., CT-301™ available from Cheltec, Inc.), safeners, bactericides, antifreeze agents, antifoams, colorants, and adhesives. Formulations of the herbicide compositions useful herein can be prepared according to any method known useful therefor in the art. Examples of thickeners (i.e., compounds which impart to the formulation modified flow properties, i.e., high viscosity in the state of rest and low viscosity in motion) are polysaccharides, such as xanthan gum (Kelzan® from Kelco), Rhodopol ® 23 (Rhone Poulenc) or Veegum ® (from R.T. Vanderbilt), and also organic and inorganic sheet minerals, such as Attaclay ® (from Engelhardt). Examples of antifoams are silicone emulsions (such as, for example, Silikon® SRE, Wacker or Rhodorsil ® from Rhodia), long-chain alcohols, fatty acids, salts of fatty acids, organofluorine compounds and mixtures thereof. Bactericides can be added for stabilizing the aqueous herbicidal formulations. Examples of bactericides are bactericides based on dichlorophen and benzyl alcohol hemiformal (Proxel® from ICI or Acticide ® RS from Thor Chemie and Kathon ® MK from Rohm & Haas), and also isothiazolinone derivatives, such as alkylisothiazolinones and benzisothiazolinones (Acticide MBS from Thor Chemie). Examples of antifreeze agents are ethylene glycol, propylene glycol, , or glycerol. Examples of colorants include members of colorant classes such as the sparingly water- soluble pigments and the water-soluble dyes. Some specific examples of these include the dyes known under the names Rhodamin B, C.I. Pigment Red 112 and C.I. Solvent Red 1, and also pigment blue 15:4, pigment blue 15:3, pigment blue 15:2, pigment blue 15:1, pigment blue 80, pigment yellow 1, pigment yellow 13, pigment red 112, pigment red 48:2, pigment red 48:1, pigment red 57:1, pigment red 53:1, pigment orange 43, pigment orange 34, pigment orange 5, pigment green 36, pigment green 7, pigment white 6, pigment brown 25, basic violet 10, basic violet 49, acid red 51, acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108. Examples of adhesives are polyvinylpyrrolidone, polyvinyl acetate, polyvinyl alcohol, and tylose. Suitable inert auxiliaries are, for example, the following: mineral oil fractions of medium to high boiling point, such as kerosene and diesel oil, furthermore coal tar oils and oils of vegetable or animal origin, aliphatic, cyclic and aromatic hydrocarbons, for example paraffin, tetrahydronaphthalene, alkylated naphthalenes and their derivatives, alkylated benzenes and their derivatives, alcohols such as methanol, ethanol, propanol, butanol and cyclohexanol, ketones such as cyclohexanone or strongly polar solvents, for example amines such as N- methylpyrrolidone, and water. Suitable carriers include liquid and solid carriers. Liquid carriers include e.g., non-aqueous solvents such as cyclic and aromatic hydrocarbons, e.g., paraffins, tetrahydronaphthalene, alkylated naphthalenes and their derivatives, alkylated benzenes and their derivatives, alcohols such as methanol, ethanol, propanol, butanol and cyc-lohexanol, ketones such as cyclohexanone, strongly polar solvents, e.g., amines such as N-methylpyrrolidone, and water as well as mixtures thereof. Solid carriers include e.g., mineral earths such as silicas, silica gels, silicates, talc, kaolin, limestone, lime, chalk, bole, loess, clay, dolomite, diatomaceous earth, calcium sulfate, magnesium sulfate and magnesium oxide, ground synthetic materials, fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate and , and products of vegetable origin, such as cereal meal, tree bark meal, wood meal and nutshell meal, cellulose powders, or other solid carriers. Suitable surfactants (adjuvants, wetting agents, tackifiers, dispersants and also emulsifiers) are the alkali metal salts, alkaline earth metal salts and ammonium salts of aromatic sulfonic acids, for example lignosulfonic acids (e.g., Borrespers-types, Borregaard), phenolsulfonic acids, naphthalenesulfonic acids (Morwet types, Akzo Nobel) and dibutylnaphthalenesulfonic acid (Nekal types, BASF AG), and of fatty acids, alkyl- and alkylarylsulfonates, alkyl sulfates, lauryl ether sulfates and fatty alcohol sulfates, and salts of sulfated hexa-, hepta- and octadecanols, and also of fatty alcohol glycol ethers, condensates of sulfonated naphthalene and its derivatives with formaldehyde, condensates of naphthalene or of the naphthalenesulfonic acids with phenol and formaldehyde, polyoxyethylene octylphenol ether, ethoxylated isooctyl-, octyl- or nonylphenol, alkylphenyl or tributylphenyl polyglycol ether, alkylaryl polyether alcohols, isotridecyl alcohol, fatty alcohol/ethylene oxide condensates, ethoxylated castor oil, polyoxyethylene alkyl ethers or polyoxypropylene alkyl ethers, lauryl alcohol polyglycol ether acetate, sorbitol esters, lignosulfite waste liquors and proteins, denatured proteins, polysaccharides (e.g., methylcellulose), hydrophobically modified starches, polyvinyl alcohol (Mowiol types, Clariant), polycarboxylates (BASF AG, Sokalan types), polyalkoxylates, polyvinylamine (BASF AG, Lupamine types), polyethyleneimine (BASF AG, Lupasol types), polyvinylpyrrolidone and copolymers thereof. Powders, materials for broadcasting and dusts can be prepared by mixing or concomitant grinding the A.I.s together with a solid carrier. Granules, for example, coated granules, impregnated granules and homogeneous granules, can be prepared by binding the A.I.s to solid carriers. Aqueous use forms can be prepared from emulsion concentrates, suspensions, pastes, wettable powders, or water-dispersible granules by adding water. To prepare emulsions, pastes or oil dispersions, the herbicidal compositions comprising an auxinic herbicide, and optionally other agronomic A.I.(s) and/or their agriculturally suitable salts and esters, either as such or dissolved in an oil or solvent, can be homogenized in water by means of a wetting agent, tackifier, dispersant or emulsifier. Alternatively, it is also possible to prepare concentrates comprising active compound, wetting agent, tackifier, dispersant or emulsifier and, if desired, solvent or oil, which are suitable for dilution with water. The concentrations of the herbicides present in the herbicidal composition comprising an auxinic herbicide, and optionally other agronomic A.I.(s) and/or their agriculturally suitable salts and esters can be varied within wide ranges. In general, the formulations comprise approximately from 0.001 to 98% by weight, preferably 0.01 to 95% by weight of at least one active ingredient. The A.I.s are employed in a purity of from 90% to 100%, preferably 95% to

100% (according to NMR spectrum). In the formulation, in some embodiments, the herbicides are present in suspended, emulsified, or dissolved form. The formulation described herein can be in the form of aqueous solutions, powders, suspensions, also highly-concentrated aqueous, oily, or other suspensions or dispersions, aqueous emulsions, aqueous microemulsions, aqueous suspo-emulsions, oil dispersions, pastes, dusts, materials for spreading or granules. In various embodiments, the herbicides can, for example, be formulated as follows: 1. Products for Dilution with Water A . Water-soluble concentrates 10 parts by weight of active compound are dissolved in 90 parts by weight of water or a water-soluble solvent. As an alternative, wetting agent(s) or other adjuvants are added. The active compound dissolves upon dilution with water. This gives a formulation with an active compound content of 10% by weight. B. Dispersible concentrates 20 parts by weight of active compound are dissolved in 70 parts by weight of cyclohexanone with addition of 10 parts by weight of a dispersant, for example polyvinylpyrrolidone. Dilution with water gives a dispersion. The active compound content is 20% by weight. C. Emulsifiable concentrates

15 parts by weight of active compound are dissolved in 75 parts by weight of an organic solvent (e.g., alkylaromatics) with addition of calcium dodecylbenzenesulfonate and castor oil ethoxylate (in each case 5 parts by weight). Dilution with water gives an emulsion. The formulation has an active compound content of 15% by weight. D. Emulsions 25 parts by weight of active compound are dissolved in 35 parts by weight of an organic solvent (e.g., alkylaromatics) with addition of calcium dodecylbenzenesulfonate and castor oil ethoxylate (in each case 5 parts by weight). This mixture is introduced into 30 parts by weight of water by means of an emulsifier (Ultraturrax) and made into a homogeneous emulsion. Dilution with water gives an emulsion. The formulation has an active compound content of 25% by weight. E. Suspensions In an agitated ball mill, 20 parts by weight of active compound are comminuted with addition of 10 parts by weight of dispersants and wetting agent(s) and 70 parts by weight of water or an organic solvent to give a fine active compound suspension. Dilution with water gives a stable suspension of the active compound. The active compound content in the formulation is 20% by weight. F. Water-dispersible granules and water-soluble granules 50 parts by weight of active compound are ground finely with addition of 50 parts by weight of dispersants and wetting agent(s) and made into water-dispersible or water-soluble granules by means of technical appliances (for example extrusion, spray tower, fluidized bed). Dilution with water gives a stable dispersion or solution of the active compound. The formulation has an active compound content of 50% by weight. G. Water-dispersible powders and water-soluble powders 75 parts by weight of active compound are ground in a rotor-stator mill with addition of 25 parts by weight of dispersants, wetting agent(s) and silica gel. Dilution with water gives a stable dispersion or solution of the active compound. The active compound content of the formulation is 75% by weight. H. Gel formulations In a ball mill, 20 parts by weight of active compound, 10 parts by weight of dispersant, 1 part by weight of gelling agent, and 70 parts by weight of water or of an organic solvent are mixed to give a fine suspension. Dilution with water gives a stable suspension with active compound

content of 20%> by weight.

Products Applied Undiluted A. Dusts 5 parts by weight of active compound are ground finely and mixed intimately with 95 parts by weight of finely divided kaolin. This gives a dusting powder with an active compound content of 5% by weight. B. Granules (GR, FG, GG, MG) 0.5 parts by weight of active compound are ground finely and associated with 99.5 parts by weight of carriers. Current methods here are extrusion, spray-drying, or the fluidized bed. This gives granules to be applied

undiluted with an active compound content of 0.5%> by weight. C. ULV solutions (UL) 10 parts by weight of active compound are dissolved in 90 parts by weight of an organic solvent, for example xylene. This gives a product to be applied undiluted with an active compound content of 10% by weight.

Aqueous use forms can be prepared from emulsion concentrates, suspensions, pastes, wettable powders, or water-dispersible granules by adding water. The herbicides or the herbicidal compositions comprising them can be applied pre-, post- emergence or pre-plant, or together with the seed. It is also possible to apply the herbicidal composition or active compounds by applying seed, pretreated with the herbicidal compositions or active compounds, of a crop plant. In a further embodiment, the herbicides or herbicidal compositions can be applied by treating seed. The treatment of seeds comprises essentially all procedures familiar to the person skilled in the art (e.g., seed dressing, seed coating, seed dusting, seed soaking, seed film coating, seed multilayer coating, seed encrusting, seed dripping and seed pelleting). In some embodiments, the herbicidal compositions can be applied diluted or undiluted. One embodiment is a method for treating a seed comprising: (a) providing a seed comprising nucleic acid of any one of SEQ ID NOs: 8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, the expression of the nucleic acid conferring to the plant or seed tolerance to an auxinic herbicide; and (b) contacting said seed with an agronomically acceptable composition. In one embodiment, the auxinic herbicides, and optionally other agronomic A.I.(s), can be mixed with a large number of representatives of other herbicidal or growth-regulating active ingredient groups and then applied concomitantly. Suitable components for mixtures are, for example, 1,2,4-thiadiazoles, 1,3,4-thiadiazoles, , aminophosphoric acid and its derivatives, aminotriazoles, anilides, (het)aryloxyalkanoic acids and their derivatives, benzoic acid and its derivatives, benzothiadiazinones, 2-aroyl-l,3-cyclohexanediones, 2-hetaroyl-l,3- cyclohexane-diones, hetaryl aryl ketones, benzylisoxazolidinones, meta-CF3-phenyl derivatives, carbamates, quinolinecarboxylic acid and its derivatives, chloroacetanilides, cyclohexenone oxime ether derivatives, diazines, dichloropropionic acid and its derivatives, dihydro- benzofurans, dihydrofuran-3-ones, dinitroanilines, dinitrophenols, diphenyl ethers, dipyridyls, halocarboxylic acids and their derivatives, ureas, 3-phenyluracils, imidazoles, imidazolinones, N- phenyl-3,4,5,6-tetrahydrophthalimides, oxadiazoles, oxiranes, phenols, aryloxy- and hetaryloxyphenoxypropionic esters, phenylacetic acid and its derivatives, 2-phenylpropionic acid and its derivatives, pyrazoles, phenylpyrazoles, pyridazines, pyridinecarboxylic acid and its derivatives, pyrimidyl ethers, sulfonamides, sulfonylureas, triazines, triazinones, triazolinones, triazolecarboxamides, , phenyl pyrazolines and isoxazolines and derivatives thereof. For the purposes of this paragraph, auxinic herbicide members are excluded from the following classes: benzoic acid and its derivatives; quinolinecarboxylic acid and its derivatives; 2- phenylpropionic acid and its derivatives; and pyridinecarboxylic acid and its derivatives. It may furthermore be beneficial to apply the herbicides alone or in combination with other herbicides, or else in the form of a mixture with other crop protection agents, for example together with agents for controlling pests or phytopathogenic fungi or bacteria. Also of interest is the miscibility with mineral salt solutions, which are employed for treating nutritional and trace element deficiencies. Other additives such as non-phytotoxic oils and oil concentrates can also be added. Moreover, it may be useful to apply the herbicides in combination with safeners. Safeners are chemical compounds, which prevent or reduce herbicide-induced injury to useful plants without having a major impact on the herbicidal action of the herbicides. They can be applied either before sowings (e.g., on seed treatments, shoots or seedlings) or in the pre- emergence application or post-emergence application of the useful plant. The safeners and the herbicides can be applied simultaneously or in succession. Suitable safeners are e.g., (quinolin-8-oxy)acetic acids, l-phenyl-5-haloalkyl-lH-l,2,4- triazol-3-carboxylic acids, l-phenyl-4,5-dihydro-5-alkyl-lH-pyrazol-3,5-dicarboxylic acids, 4,5- dihydro-5,5-diaryl-3-isoxazol carboxylic acids, dichloroacetamides, alpha- oximinophenylacetonitriles, acetophenonoximes, 4,6-dihalo-2-phenylpyrimidines, N-[[4- (aminocarbonyl)phenyl]sulfonyl]-2-benzoic amides, 1,8-naphthalic anhydride, 2-halo-4- (haloalkyl)-5-thiazol carboxylic acids, phosphorthiolates and N-alkyl-O-phenyl-carbamates and their agriculturally acceptable salts and their agriculturally acceptable derivatives such amides, esters, and thioesters, provided they have an acid group. Examples of safeners are benoxacor, cloquintocet, cyometrinil, cyprosulfamide, dichlormid, dicyclonon, dietholate, fenchlorazole, fenclorim, flurazole, fluxofenim, furilazole, isoxadifen, mefenpyr, mephenate, naphthalic anhydride, oxabetrinil, 4-(dichloroacetyl)-l-oxa-4- azaspiro[4.5]decane (MON4660, CAS 71526-07-3) and 2,2,5-trimethyl-3-(dichloroacetyl)-l,3- oxazolidine (R-29148, CAS 52836-31-4). Those skilled in the art will recognize that some of the above mentioned herbicides and/or safeners are capable of forming geometrical isomers, for example E/Z isomers. It is possible to use both, the pure isomers and mixtures thereof, in the compositions described herein. Furthermore, some of the above mentioned herbicides and/or safeners have one or more centers of chirality and, as a consequence, are present as enantiomers or diastereomers. It is possible to use both, the pure enantiomers and diastereomers and their mixtures, in the compositions described herein. In particular, some of the aryloxyphenoxy propionate herbicides are chiral, and some of them are commonly used in enantiomerically enriched or enantiopure form, e.g., clodinafop, cyhalofop, fenoxaprop-P, fluazifop-P, haloxyfop-P, metamifop, propaquizafop or quizalofop-P. As a further example, glufosinate may be used in enantiomerically enriched or enantiopure form, also known as glufosinate-P. Those skilled in the art will recognize that any derivative of the above mentioned herbicides and/or safeners can be used in the practice of the embodiments or aspects described herein, for example agriculturally suitable salts and esters.

Methods for Controlling Weeds Herbicide-tolerant plants described herein can be used in conjunction with an herbicide to which they are tolerant. Herbicides can be applied to the plants described herein using any techniques known to those skilled in the art. Herbicides can be applied at any point in the plant cultivation process. For example, herbicides can be applied pre-planting, at planting, pre- emergence, post-emergence or combinations thereof. Herbicides may be applied to seeds and dried to form a layer on the seeds. One embodiment described herein is a method for controlling weeds at a locus for growth of a plant or plant part thereof, the method comprising: applying a composition comprising an auxinic herbicide to the locus. In one aspect, the locus for growth of a plant is a field. In one aspect, the plant has the auxinic herbicide-tolerance characteristic of any of Sinapis arvensis lines DT-01 SA2-R or DT-01 BC8SA2-R. In another aspect, the plant has the auxinic herbicide- tolerance characteristic DART. One embodiment described herein is a method for controlling weeds in a field by an application of an auxinic herbicide without significantly inhibiting the growth of a Brassica plant, the method comprising: (a) providing a Brassica plant or seed comprising the nucleic acid of: (i) a chimeric polynucleotide comprising both a Sinapis arvensis polynucleotide portion and a Brassica polynucleotide portion, wherein said chimeric polynucleotide encodes the DART trait of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent

Deposit Designation Numbers PTA-120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA-1 1213, and PTA-1 1214, respectively; or (ii) a mutagenized or recombinant polynucleotide encoding the DART trait of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with the ATCC under Patent Deposit Designation Numbers

PTA-120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA-1 1213, and PTA-1 1214, respectively; the expression of said nucleic acid conferring to the plant or seed tolerance to an auxinic herbicide; and (b) applying an herbicide composition comprising an effective amount of an auxinic herbicide: (i) to the field, followed by planting of said plant or seed in therein; (ii) to the field, during or after planting of said seed therein; (iii) to the plant in said field and to weeds in the vicinity of the plant; (iv) to said seed, followed by planting of said seed in the field; or (v) to a plant by the seed after it has been planted in the field, and to weeds in the vicinity of the plant; wherein said effective amount of said auxinic herbicide would significantly inhibit the growth of a corresponding wild-type variety of said Brassica; thereby controlling weeds. In one aspect, nucleic acid comprises the DART auxinic herbicide-tolerance trait. In another aspect, the herbicide composition is applied to the weeds and to the plant, seed, or the plant produced by the seed. Another embodiment described herein is a method for controlling weeds in a field by application of an auxinic herbicide without significantly inhibiting the growth of a Brassica plant, the method comprising: (a) providing a Brassica plant or seed comprising the nucleic acid of any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, the expression of the nucleic acid conferring to the plant or seed tolerance to an auxinic herbicide; and (b) applying an herbicide composition comprising an effective amount of an auxinic herbicide: (i) to the field, followed by planting of said plant or seed in therein; (ii) to the field, during or after planting of said seed therein; (iii) to the plant in said field and to weeds in the vicinity of the plant; (iv) to said seed, followed by planting of said seed in the field; or (v) to a plant by the seed after it has been planted in the field, and to weeds in the vicinity of the plant; wherein the effective amount would significantly inhibit the growth of a corresponding wild-type variety of said Brassica; thereby controlling weeds. In one aspect, nucleic acid comprises the DART auxinic herbicide-tolerance trait. Another embodiment described herein is a method for combating undesired vegetation in a field comprising contacting a seed of a crop plant before sowing and/or after pregermination with an auxinic herbicide composition without significantly inhibiting the growth of said crop plant. In one aspect, the seed comprises the DART auxinic herbicide-tolerance trait. In another aspect, the auxinic herbicide composition is applied to the weeds and to the plant, seed, or the plant produced by the seed. Herbicide compositions described herein can be applied, e.g., as foliar treatments, soil treatments, seed treatments, or soil drenches. Application can be made, e.g., by spraying, dusting, broadcasting, or any other mode known useful in the art. In one embodiment, herbicides can be used to control the growth of weeds that may be found growing in the vicinity of the herbicide -tolerant plants described herein. In embodiments of this type, an herbicide can be applied to a plot in which herbicide-tolerant plants described herein are growing in vicinity to weeds. An herbicide to which the herbicide-tolerant plant described herein is tolerant can then be applied to the plot at a concentration sufficient to kill or inhibit the growth of the weed. Concentrations of herbicide sufficient to kill or inhibit the growth of weeds are known in the art and are disclosed above. Anther embodiment described herein is a method for controlling weeds in the vicinity of an auxinic herbicide -tolerant plant as described herein. The method comprises applying an effective amount of an auxinic herbicide to the weeds and to the auxinic herbicide -tolerant plant, wherein the plant has increased tolerance to auxinic herbicide when compared to a wild-type plant. In some embodiments, the auxinic herbicide-tolerant plants described herein are preferably crop plants, including, but not limited to, sunflower, alfalfa, Brassica sp., soybean, cotton, safflower, peanut, tobacco, tomato, potato, wheat, rice, maize, sorghum, barley, rye, millet, and sorghum. Another embodiment described herein is a method for controlling weeds in a field or crops by use of an auxinic herbicide without significantly inhibiting the growth of the crop plant, the method comprising: (a) providing a seed-treatment-treated seed comprising the nucleic acid of any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, the expression of the nucleic acid conferring to the plant or seed tolerance to an auxinic herbicide, the seed treatment comprising an auxinic herbicide; and (b) planting said treated seed in the field. In other embodiments, herbicide(s) (e.g., auxinic herbicides) can be used as a seed treatment. In some embodiments, an effective concentration or an effective amount of herbicide(s), or a composition comprising an effective concentration or an effective amount of herbicide(s) can be applied directly to the seeds prior to or during the sowing of the seeds. Such compositions are described herein as seed treatment compositions. In one aspect, the seed treatment composition comprises an auxinic herbicide. In one embodiment, the seed has, disposed on a surface thereof, a seed treatment composition comprising at least one agronomically acceptable ingredient. In one aspect, the ingredient is at least one agronomically acceptable herbicide, fungicide, nematicide, or insecticide, or a combination thereof. In another aspect, the said insecticide comprises at least one anti-coleopteran agent, anti-hemipteran agent, anti-lepidopteran agent, or a combination thereof. Another embodiment described herein is a method for treating the seed with an agronomically acceptable composition, the method comprising contacting the seed with the agronomically acceptable composition, wherein the composition comprises an auxinic herbicide. Another embodiment described herein is a method for preparing a treated seed, the method comprising: providing a seed and applying thereto a seed treatment composition. Seed treatment formulations may additionally comprise binders and optionally colorants. Binders can be added to improve the adhesion of the active materials on the seeds after treatment. In one embodiment, suitable binders are block copolymers EO/PO surfactants but also polyvinylalcohols, polyvinylpyrrolidones, polyacrylates, polymethacrylates, polybutenes, polyisobutylenes, polystyrene, polyethyleneamines, polyethyleneamides, polyethyleneimines (Lupasol®, Polymin®), polyethers, polyurethanes, polyvinylacetate, tylose and copolymers derived from these polymers. Optionally, also colorants can be included in the formulation. Suitable colorants or dyes for seed treatment formulations are Rhodamin B, C.I. Pigment Red

112, C.I. Solvent Red 1, pigment blue 15:4, pigment blue 15:3, pigment blue 15:2, pigment blue

15:1, pigment blue 80, pigment yellow 1, pigment yellow 13, pigment red 112, pigment red 48:2, pigment red 48:1, pigment red 57:1, pigment red 53:1, pigment orange 43, pigment orange 34, pigment orange 5, pigment green 36, pigment green 7, pigment white 6, pigment brown 25, basic violet 10, basic violet 49, acid red 51, acid red 52, acid red 14, acid blue 9, acid yellow 23, basic red 10, basic red 108. The phrase "seed treatment" comprises all suitable seed treatment techniques known in the art, such as seed dressing, seed coating, seed dusting, seed soaking, and seed pelleting. One embodiment described herein is a method of treating soil by the application, in particular into the seed drill: either of a granular formulation containing the auxinic herbicide as a composition/formulation (e.g., a granular formulation), with optionally one or more solid or liquid, agriculturally acceptable carriers and/or optionally with one or more agriculturally acceptable surfactants. This method is advantageously employed, for example, in seedbeds of cereals, maize, cotton, and sunflower. Another embodiment described herein comprises seeds coated with and/or containing a seed treatment formulation comprising auxinic herbicide and at least one other herbicide such as, e.g., an AHAS-inhibitor consisting of amidosulfuron, azimsulfuron, bensulfuron, chlorimuron, chlorsulfuron, cinosulfuron, cyclosulfamuron, ethametsulfuron, ethoxysulfuron, , flupyrsulfuron, foramsulfuron, halosulfuron, imazosulfuron, iodosulfuron, mesosulfuron, metsulfuron, nicosulfuron, oxasulfuron, primisulfuron, prosulfuron, pyrazosulfuron, rimsulfuron, sulfometuron, sulfosulfuron, thifensulfuron, triasulfuron, tribenuron, trifloxysulfuron, triflusulfuron, tritosulfuron, imazamethabenz, imazamox, imazapic, imazapyr, imazaquin, imazethapyr, cloransulam, diclosulam, florasulam, flumetsulam, metosulam, penoxsulam, bispyribac, pyriminobac, propoxycarbazone, flucarbazone, pyribenzoxim, pyriftalid and pyrithiobac. The term "coated with and/or containing" generally signifies that the active ingredient is for the most part on the surface of the propagation product at the time of application, although a greater or lesser part of the ingredient may penetrate into the propagation product, depending on the method of application. When the said propagation product is (re)planted, it may absorb the active ingredient. In some embodiments, the seed treatment application with auxinic herbicide or with a formulation comprising the auxinic herbicide is carried out by spraying or dusting the seeds before sowing and before emergence of the plants. In other embodiments, in the treatment of seeds, the corresponding formulations are applied by treating the seeds with an effective amount of auxinic herbicide or a formulation comprising the auxinic herbicide. Another aspect described herein is a method for combating undesired vegetation or controlling weeds comprising: contacting the seeds of the auxinic herbicide-tolerant plants described herein before sowing and/or after pregermination with auxinic herbicide. The method can further comprise sowing the seeds, for example, in soil, in a field, or in a potting medium in a greenhouse. The method finds particular use in combating undesired vegetation or controlling weeds in the immediate vicinity of the seed. The control of undesired vegetation is understood as the killing of weeds and/or otherwise retarding or inhibiting the normal growth of the weeds. Weeds, in the broadest sense, are understood as meaning all those plants that grow in locations where they are undesired. The weeds described herein include, for example, dicotyledonous and monocotyledonous weeds. Dicotyledonous weeds include, but are not limited to, weeds of the genera: Sinapis, Lepidium, Galium, Stellaria, Matricaria, Anthemis, Galinsoga, Chenopodium, Urtica, Senecio, Amaranthus, Portulaca, Xanthium, Convolvulus, Ipomoea, Polygonum, Sesbania, Ambrosia, Cirsium, Carduus, Sonchus, Solanum, Rorippa, Rotala, Lindernia, Lamium, Veronica, Abutilon, Emex, Datura, Viola, Galeopsis, Papaver, Centaurea, Trifolium, Ranunculus, and Taraxacum. Monocotyledonous weeds include, but are not limited to, weeds of the genera: Echinochloa, Setaria, Panicum, Digitaria, Phleum, Poa, Festuca, Eleusine, Brachiaria, Lolium, Bromus, Avena, Cyperus, Sorghum, Agropyron, Cynodon, Monochoria, Fimbristyslis, Sagittaria, Eleocharis, Scirpus, Paspalum, Ischaemum, Sphenoclea, Dactyloctenium, Agrostis, Alopecurus, and Apera. In addition, the weeds described herein can include, for example, crop plants that are growing in an undesired location. For example, a volunteer maize plant in a field that predominantly comprises soybean plants can be considered a weed, if the maize plant is undesired in the field of soybean plants. Other embodiments described herein are nucleic acids and proteins providing auxin herbicide tolerance or resistance to a plant. Such nucleic acids encode polypeptides conferring auxin herbicide tolerance to plants. In one aspect, these nucleic acids encode functional polypeptides that confer the DART auxin herbicide tolerance trait. Other embodiments described herein are auxin herbicide susceptible nucleic acids that can be mutagenized to create auxin herbicide tolerant plants. Without being limited by any theory, in one aspect, the mutagenized nucleic acids encode functional polypeptides that confer the DART auxin herbicide tolerance trait. One embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame ¾r_R_8134.g27742.tl (SEQ ID NO:8), and encodes a translation elongation factor EF1A/initiation factor family protein (SEQ ID NO:9) to provide the auxin herbicide tolerance. See Table 7. In another aspect, the nucleic acid of SEQ ID NO:8 that encodes the polypeptide of SEQ ID NO:9 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises SEQ ID NO: 10 and encodes a translation elongation factor EF1A/initiation factor family protein (SEQ ID NO: 11). In one aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. TCAGATTCATTAAGTCAACTTAATCTTGCCATCGTTGGACATGTTGATTCCGGTAAATC AACACTTTCTGGTAGACTACTGCATCTATTGGGAAGAATATCTCAAAAGCAAATGCACA AGTTTGAGAAAGAAGCAAAGTTGCAGGGCAAAGGGTCCTTTGCATATGCCTGGGCATTG GATGAGAGTACTGAAGAGAGGGAACGAGGAATAACAATGACAGTGGCAGTTGCTTATTT CAACACCAAAAGACATCATGTTGTTT TGCTAGACTCTCCTGGACACACAGACTTTGTTC CGAACATGATAGCAGGGGCAACACAGGCAGATGCTGCGATTCTCGTCGTAGATGCATCT ATCGGTGCTTTTGAAGCTGGTTTTGATAATTTGAAAGGCCAGACAAGGGAGCATGCACG TGTTCTTAGAGGCTTTGGCGTGGAGCAAGTCATCGTTGCAGTCAACAAAATGGATATTG TTGGGTATTCCAAGGACAGGTTTGATTTGATTAAGCAGCATGTTGGATCTTTTCTGCAG TCATGTCGCTTTAAAGAGTCGTGTCTGACATGGATTCCGTTAAGTGCCATGGAAAACCA AAACTTGGTTTCAGCTCCCTCTGAAAGCCGCCTATCCTCATGGTATCAAGGTCCATGTT TATTGGATGCAATTGACTCTGTCAACTCTCCTGATAGAGACGTATCAAAGCCTCTGCTC ATGCCTATATGTGACGTTGTAAGATCAACCTCGCAAGGGCAGGTATCTGCATGTGGCAA ACTTGAAGCTGGAGCTGTTCGGCCAGGATCCAAGATAATGGTTATGCCATCGGGAGATC AAGGAACCGTGCGGTCTCTGGAGCGTGACTCTCAGGGTTGCACCATCGCAAGAGCTGGA GATAACGTAGCAATAGCTTTGCAAGGGATCGATGCAAATCAAGTGATGGCAGGAGGTGT GCTGTGCCATCCTGATTACCCGGTTTCAGTAGCAACTCATTTAGAACTGATGGTGCTCG TCTTGGAAGGTGCAACACCGATCTTGCTCGGTTCTCAGTTGGAGTTTCATGTCCACCAT GCAAAGGAAGCAGCAACAGTTGTGAAACTTGTGGCAATGCTAGATCCCAAAACAGGGGA GCCGACAAAGAAGTCTCCTCGTTGTCTAACTGCTAAACAGAGTGCAATGCTCGAGGTGA GTCTTCAGTATCCAGTTTGTGTGGAGACATTTTCTGAAAGTAGAGCTCTTGGAAGAGTG TTCCTTAGATCATCAGGAAGAACAGTGGCAATGGGCAAAGTTACTCGGATCATCCAAGA TTCATAA MSLFPEVTWLSYCYMLDKS SDFAI SSAFLTLTASMKQKEKQDRAEQKVPLKKERDRSET SSQGRHAHVAGVGGIRSGKSLPKAKADTSNETNS SSKHMEASESLTGTMNKMSLTGETE SSRDVKIRSARSKSKHKPEERMLLDKESDSLSQLNLAIVGHVDSGKSTLSGRLLHLLGR I SQKQMHKFEKEAKLQGKGSFAYAWALDESTEERERGI TMTVAVAYFNTKRHHWLLDS PGHTDFVPNMIAGATQADAAI LWDAS IGAFEAGFDNLKGQTREHARVLRGFGVEQVIV RAA AVNKMDIVGYSKDRFDLIKQHVGSFLQSCRFKESCLTWI PLSAMENQNLVSAPSESRLS 9 SWYQGPCLLDAI DSVNS PDRDVSKPLLMPICDWRSTSQGQVSACGKLEAGAVRPGSKI MVMPSGDQGTVRSLERDSQGCT IARAGDNVAIALQGI DANQVMAGGVLCHPDYPVSVAT HLELMVLVLEGATPI LLGSQLEFHVHHAKEAATWKLVAMLDPKTGEPTKKS PRCLTAK QSAMLEVSLQYPVCVETFSESRALGRVFLRS SGRTVAMGKVTRI IQDS

Sar_S_5332.gl3823.tl: Translation elongation factor EF1A/initiation factor family protein ATGCCTCGCAAAGGATTCTCCAATTTCGAT GACTACGATGATGGTTTAGACGATGACGA TGCGTATGATTATGACTATGATGATGATGATGATGATGATGATGAACATGAAGCTGCTG AACCAAAGGAAGTAGAAGTAAATGATCGGCAAGGGATTTGGAGATGTGGAATCTGTACA TATGACAATGATGAGAGTATGAATGTGTGTGAGATTTGCGGTTCTATTCGTCATCCAGT GGCTGGTGGAAACCAAACTATCAATAACAATACAGCAAGCATGAAGCAGAAAGAGAGGC AGTACATGTCAGAACAGAATCCTGTAAAGAAAGAAAGAGATAGATCAGAAACTAGCTCT CAAGGTAGACACGCCAATGTTGGCGGCATAAAATCTGGCAAAAGTTTGCCAAAATCAAA AGCAGATACGTCTAATGAGACAAATTCTTCGTCAAAAAATATGGAGGCGTCAGAGAGTC TTACTGGTACTATGAACAAAATGTCTTTGACTGGGGAAACAGAAAGCTCCAGACATATT AAAATTAGAAGCGCAAGATCAAAATCAAACCATAAGCCAGAGGAATGGATGCTCCTTGA S NT 10 TAAAGAATCAGATACACTAAGTCAACTTAATCTTGCCATCGTTGGACATGTTGATTCCG GTAAATCAACACTTTCTGGTAGACTACTGCATCTATTGGGAAGAATATCTCAAAAGCAA ATGCACAAGTTTGAGAAAGAAGCAAAGTTGCAGGGCAAAGGGTCCTTTGCATATGCCTG GGCATTGGATGAGAGTACTGAAGAGAGGGAACGAGGAATAACAATGACAGTGGCAGTTG CTTATTTCAACACCAAAAGACATCATGTTGTTT TGCTAGACTCTCCTGGACACACAGAC TTTGTTCCGAACATGATAGCAGGGGCAACACAGGCAGATGCTGCGATTCTCGTCGTAGA TGCATCTATCGGTGCTTTTGAAGCTGGTTTTGATAATTTGAAAGGCCAGACAAGGGAGC ATGCACGTGTTCTTAGAGGCTTTGGCGTGGAGCAAGTCATCGTTGCAGTCAACAAAATG GATATTGTTGGGTATTCCAAGGACAGGTTTGATTTGATTAAGCAGCATGTTGGAACTTT TCTGCAGTCATGTCGCTTTAAAGAGTCGTGTCTGACATGGATTCCGTTAAGTGCCATGG AAAACCAAAACTTGGTTTCAGCTCCCTCTGAAAGCCGCCTATCCTCATGGTATCAAGGT CCATGTTTATTGGATGCAATTGACTCTGTCAACTCTCCTGATAGAGACGTATCAAAGCC TCTGCTCATGCCTATATGTGACGTTGTAAGATCAACTTCACAAGGGCAGGTATCTGCAT GTGGCAAACTTGGAGCTGGAGCTGTTCGGCCAGGATCCAAGATAATGGTTATGCCATCG GGAGATCAAGGAACCGTGCGGTCTCTGGAGCGTGACTCTCAGGGTTGCACCATCGCAAG AGCTGGAGATAACGTAGCAATAGCTTTGCAAGGGATCGATGCAAATCAAGTGATGGCAG GAGGTGTGCTGTGCCATCCTGATTACCCGGTTTCAGTAGCAACTCATTTAGAACTGATG GTGCTCGTCTTGGAAGGTGCAACACCGATCTTGCTCGGTTCTCAGTTGGAGTTTCATGT GCACCATGCAAAGGAAGCAGCAACAGTTGTGAAACTTGTGGCAATGCTTGATCCCAAAA CAGGGGAGCCGACAAAGAAGTCTCCTCGTTGTCTAACTGCTAAACAGAGTGCAATGCTT GAGGTGAGTCTTCAGTATCCAGTTTGTGTGGAGACATTTTCTGAAAGTAGAGCTCTTGG AAGAGTGTTCCTTAGATCATCAGGAAGAACAGTGGCAATGGGCAAAGTTACTCGGATCA TCCAAGATTCATAA MPRKGFSNFDDYDDGLDDDDAYDYDYDDDDDDDDEHEAAEPKEVEVNDRQGIWRCGICT YDNDESMNVCE ICGS IRHPVAGGNQT INNNTASMKQKERQYMSEQNPVKKERDRSETS S QGRHANVGGIKSGKSLPKSKADTSNETNS S SKNMEASESLTGTMNKMSLTGETES SRHI KIRSARSKSNHKPEEWMLLDKESDTLSQLNLAIVGHVDSGKSTLSGRLLHLLGRI SQKQ MHKFEKEAKLQGKGSFAYAWALDESTEERERGI TMTVAVAYFNTKRHHWLLDS PGHTD 1 S AA FVPNMIAGATQADAAI LWDAS IGAFEAGFDNLKGQTREHARVLRGFGVEQVIVAVNKM 1 1 DIVGYSKDRFDLIKQHVGTFLQSCRFKESCLTWI PLSAMENQNLVSAPSESRLS S YQG PCLLDAI DSVNS PDRDVSKPLLMPICDWRSTSQGQVSACGKLGAGAVRPGSKIMVMPS GDQGTVRSLERDSQGCT IARAGDNVAIALQGI DANQVMAGGVLCHPDYPVSVATHLELM VLVLEGATPI LLGSQLEFHVHHAKEAATWKLVAMLDPKTGEPTKKS PRCLTAKQSAML EVSLQYPVCVETFSESRALGRVFLRS SGRTVAMGKVTRI IQDS

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame Sar_R_2008.g8030.tl (SEQ ID NO: 12), and encodes a KAT2 peroxisomal 3- ketoacyl-CoA thiolase 3 (SEQ ID NO: 13) to provide the auxin herbicide tolerance. See Table 8. In another aspect, the nucleic acid of SEQ ID NO: 12 that encodes the polypeptide of SEQ ID

NO: 13 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_22769.g45642.tl (SEQ ID NO: 14), and encodes a KAT2 peroxisomal 3-ketoacyl-CoA thiolase 3 (SEQ ID NO: 15). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. Table 8. S. arvensis Auxin-Herbicide Tolerance Trait Sequence Gene 2 Nucleic Acid or Amino Acid Sequence SEQ ID Sar R 2008.g8030.tl: KAT2 peroxisomal 3-ketoacyl-CoA thiolase 3 ATGGAGAAAGCGATCGAGAGGCAACGAGTTCTTCTTGAACATCTCCGTCCTTCTTCTTC CTCCTCCTCCCACAGTTTTGAAGGCTCTCTCTCTGCTTCAGCTTGCTTGGCTGGGGATA GTGCTGCGTATCAGAGGACCTCTCTCTATGGAGATGATGTAGTCATTGTTGCGGCACAT AGGACTGCACTATGCAAGTCGAAACGTGGCAATTTCAAGGATACTTATCCGGATGATCT CCTCGCCCCGGTTTTGAGGGCATTGATAGAGAAGACAAATCTTAACCCAAGTGATGTTG GTGACATTGTTGTCGGTACTGTTTTGGCACCGGGATCTCAGAGAGCCAGCGAATGCAGG ATGTCTGCTTTCTATGCCGGTTTCCCTGAAACCGTTCCGGTTAGAACCGTGAATAGACA GTGCTCTTCTGGGCTTCAGGCTGTTGCTGACGTAGCCGCTGCCATCAAAGCTGGATTTT ATGATATTGGTATTGGGGCTGGATTGGAGTCCATGACTACTAATCCGATGGCATGGGAA GGGTCAGTCAACCCTGCGGTGAAGAAGTTTGAGCAAGCACAGAATTGTCTTCTCCCTAT GGGTGTTACTTCAGAAAATGTAGCACAGCGCTTTGGTGTCTCAAGGCAGGAGCAAGATC AAGCTGCTGTTGACTCGCACAGAAAGGCAGCTGCTGCAACTGCTGCTGGTAAATTCAAG 12 RNT GATGAGATCATTCCGGT CAAAACCAAGCTTGTTGACCCAAAGACAGGCGATGAGAAACC CATTACAGTTTCTGTTGATGATGGGATCCGACCAACCACAACCCTTGCTACTCTTGGGA AGCTGAAGCCAGTCTTTAAAAAGGATGGAACCACAACAGCTGGAAATTCCAGCCAAGTT AGTGATGGTGCAGGAGCGGTTCTCCTCATGAGGAGAAGTGTTGCTACGCAGAAAGGACT TCCCGTTCTTGGGGTATTCAGGACATTTGCTGCAGTTGGTGTTGACCCAGCAATCATGG GTGTTGGTCCAGCAGTTGCAATTCCTGCTGCAGTTAAGGCGGCTGGTTTAGAACTCGAT GACATCGACTTGTTTGAGATCAACGAGGCATTTGCATCTCAGTTTGTTTATTGCCGTAA CAAGTTGGGACTTGACCCGGAGAAAATCAATGTCAACGGTGGCGCAATGGCCATAGGAC ATCCTTTGGGTGCTACAGGAGCACGTTGTGTTGCTACTTTGTTGCACGAGATGAAACGC CGTGGAAAAGACTGTCGATTTGGGGTAGTGTCAATGTGCATTGGGACGGGGATGGGTGC AGCGGCTGTGTTTGAGAGAGGAGATGGAGTAGATGAGCTTCGCAACGCAAGAAAAGTTG AATCGCAAGGCTTTTTGTCCAAGGACGCTCGTTAG MEKAIERQRVLLEHLRPS SSSSSHSFEGSLSASACLAGDSAAYQRTSLYGDDWIVAAH RTALCKSKRGNFKDTYPDDLLAPVLRALIEKTNLNPSDVGDIWGTVLAPGSQRASECR MSAFYAGFPETVPVRTVNRQCS SGLQAVADVAAAIKAGFYDIGIGAGLESMTTNPMAWE GSVNPAVKKFEQAQNCLLPMGVTSENVAQRFGVSRQEQDQAAVDSHRKAAAATAAGKFK 2 RAA 13 DE PVKTKLVDPKTGDEKPI TVSVDDGIRPTTTLATLGKLKPVFKKDGTTTAGNS SQV SDGAGAVLLMRRSVATQKGLPVLGVFRTFAAVGVDPAIMGVGPAVAI PAAVKAAGLELD DI DLFE INEAFASQFVYCRNKLGLDPEKINVNGGAMAIGHPLGATGARCVATLLHEMKR RGKDCRFGWSMC IGTGMGAAAVFERGDGVDELRNARKVESQGFLSKDAR Sar S 22769.g45642.tl: KAT2 peroxisomal 3-ketoacyl-CoA thiolase 3 ATGGAGAAAGCAATAGAGAGACAACGAGTTCTCCTTGAACATCTCCGTCCTTCTTCCTC CTCCTCGCACAGTTTCGAAGGCTCTCTCTCTGCCTCTGCTTGCTTGGCTGGAGACAGTG CTGCATATCAGAGGACCTCTCTCTATGGAGATGATGTTGTCATTGTCGCGGCGCATAGG ACAGCTCTGTGCAAGTCCAAACGTGGCAACTTCAAGGATACTTATCCTGATGATCTCCT TGCACCTGTTTTGAGGGCATTGATAGAGAAGACAAATCTAGACCCAAGTGAAGTTGGTG ACATTGTTGTGGGTACTGTTTTGGCTCCGGGGTCTCAGAGAGCCAGCGAATGCAGGATG TCTGCTTTCTATGCTGGTTTCCCCGAAACCGTGGCCGTGAGGACCGTGAATAGACAGTG TTCCTCTGGGCTTCAGGCTGTTGCTGACGTGGCTGCTGCCATCAAAGCTGGATTTTATG ATATTGGTATTGGGGCTGGACTGGAGTCCATGACTACCAATCCGATGGCATGGGAAGGG TCTGTCAACCCAGCGGTGAAGAAGTTTGCGCAAGCACAGAGTTGTCTTCTCCCTATGGG 14 2 S NT TGTTACTTCAGAAAATGTAGCACACCGCTTTGGTGTCTCGAGGCAGGAGCAAGATCAAG CTGCTGTCGACTCGCACAGAAAGGCAGCTGCTGCTACTGCTGCTGGTAAATTCAAGGAT GAGATCATTCCTGTT CAAACCAAGCTTGTTGACCCAAAGACAGGTGATGAGACACCCAT AACAGTTTCTGTTGATGATGGGATCCGACCAAGCACAACCCTTGCTACTCTTGGGAAGC TGAAGCCTGTGTTTAAAAAGGATGGCACCACAACTGCTGGAAACTCCAGTCAAGTAAGT GATGGTGCAGGAGCTGTTCTCCTCATGAAGAGAAGTGTTGCTATGCAAAAAGGACTTTC CGTTCTTGGTGTATTCAGGACATTTGCTGCAGTTGGTGTTGATCCAGCAATCATGGGTG TCGGTCCAGCAGTTGCCATCCCAGCTGCAGTTAAGGCAGCTGGTTTAGAGCTCGATGAC ATCGACTTGTTTGAGATTAACGAGGCATTTGCATCTCAGTTTTTGTATTGCCGGAACAA GTTGGGACTTGACCCAGAGAAGGTCAATGTCAACGGTGGCGCTATGGCCATTGGACATC CTTTGGGCGCTACAGGAGCACGTTGCGTTGCTACATTGTTGCACGAGATGAAGCGCCGT GGAAAAGACTGTCGATTTGGGGTAGTGTCAATGTGCATTGGGACGGGGATGGGTGCAGC AGCTGTGTTCGAGAGAGGAGATGGAGTTGATGAGCTTCGCAACGCAAGAAAAGTTGAAG GGCAAGGCTATTTGTCCAAGGACGCTCGTTAG MEKAIERQRVLLEHLRPS SSSSHSFEGSLSASACLAGDSAAYQRTSLYGDDWIVAAHR TALCKSKRGNFKDTYPDDLLAPVLRALIEKTNLDPSEVGDIWGTVLAPGSQRASECRM SAFYAGFPETVAVRTVNRQCSSGLQAVADVAAAIKAGFYDIGIGAGLESMTTNPMAWEG SVNPAVKKFAQAQSCLLPMGVT SENVAHRFGVSRQEQDQAAVDSHRKAAAATAAGKFKD 15 2 S AA E PVQTKLVDPKTGDETPI TVSVDDGIRPSTTLATLGKLKPVFKKDGTTTAGNS SQVS DGAGAVLLMKRSVAMQKGLSVLGVFRTFAAVGVDPAIMGVGPAVAIPAAVKAAGLELDD IDLFE INEAFASQFLYCRNKLGLDPEKVNVNGGAMAIGHPLGATGARCVATLLHEMKRR GKDCRFGWSMC IGTGMGAAAVFERGDGVDELRNARKVEGQGYLSKDAR

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame is 5ar_R_942.g3734.tl (SEQ ID NO: 16), and encodes a calmodulin 5-like protein that facilitates protein-protein interactions (SEQ ID NO: 17) to provide the auxin herbicide tolerance. See Table 9. No auxin herbicide susceptible counterpart was identified. In another aspect, the nucleic acid of SEQ ID NO: 16 that encodes the polypeptide of SEQ ID NO: 17 provides the DART auxinic herbicide tolerance trait to a plant.

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame Sar_R_6352.g22471.tl (SEQ ID NO:18), and encodes an ARF21 transcription factor (SEQ ID NO: 19) to provide the auxin herbicide tolerance.. See Table 10. In another aspect, the nucleic acid of SEQ ID NO: 18 that encodes the polypeptide of SEQ ID NO: 19 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_14769.g33259.tl (SEQ ID NO:20), and encodes an ARF21 transcription factor (SEQ ID NO:21). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. KVRWSNEERENELEPKEQLVAEDATLNNGWKQEEYIGKKGGTARRDTSTTIQDIPTSS Sar S 14769.g33259.tl: ARF21 transcription factor ATGGCAAGTGATCAAATCATGCATGCGCAACCCGGAGTTTCAGCTATTGATGGAAGCAA TAACTATTTGAACGGTCAATTATGGAAGTTATGTGCTGGACCTCTTTTTGATACTCCAA AAATTGGAGAGAAAG AGAGACCAAAACCGA GAAATTTATGCGGAAGTTTCTTTGTTG CCTGATATATCTGATATTGAAATCCCTATTCCTAAAAACGAAAACACCATACAAAACAT TAACTATTTCACCAAGGTGTTAAGTGTTTCTGATACCCAAAAAAGTGGTGGTTTTGTTT TGTATAAAAGACATGCCATTGAATGTCTTCCCCTGCTGGACATGTCCCAGCCAACCCCA AATCAAGATATAATTGCTAAAGATATTCATGGTCAGGAATGGAGTTTTAAACACACTTT AAGAGGTACACCAAAAAGACATATTTTCGCATCTGGTTGGAATGAGTTTGCAAAACAAA AACATTTGGTTGCTGGAGACTCTTTTGTATTCCTTCGAGGAGAGAATGGGGAATCACGG GTTGGAATCAGTAAAGCAGCTCATCAGCAACGCAACATAGTAACATCTTTAATGTCAAA ACAGAGTATGCACCATGGTGTAGTTGCTACTGCACTGAATGCTATTAAGAACAAATGTA TGTTCGTTGTGTTCTATAAGCCACGGTCGAGTCAATTCCTTGTAAACATCAATAAATTT GTAGATGTAGTGAATAAGAAGTTTAGTAGAGGC TCCAAAT TTTCAATGAAATTCGAAGG 4 SNT AAAAAAT TTAAATGAAATAAGATACAATGGAACAATAGTAGGAGTGAGAGATTTCTCCA 20 CTCATTGGAAGGATTCAGAATGGCGAAGTCTAGAAGTGCAATGGGATGAAGCTGCCACT ATTCCAAGACCTGATAAGG TATCTCCATGGGAGATTGAGCTGTTAACACATTCCTCAAA CATTTTCGAGTCAGATTATCTAAAACATAAACGTCAAATCAAAGTACATGAGTTTGGTT CAAAAATGGGGGCTCCTATCTTGTCCCAAGGCCCAGAAAATGGACAGTCGACGATACAA TCTTCCATGAGATACTCATTCCCTACCATGTCCAAACCAAACTACAATGAGAAAATGGT TCAAGCAATGAAAGAAACGTCAACCACAACCGCAAGTACTAGCTGCAGACTGTTTGGAG TTAATCTAACGGTTCCCACGACAGCAAAGGATCCAACGGAACCTATTGACACCTACAAA AAATTAAAGATGTCTAATATCTTT GAAGAAGAAAAGGC TGACCATGTCCAAGC TAGAAT TCATACTAAGGTTCATATGGAAGGTGATGTAATCCTGATCGAACCAGAAAACAATGCAA ACATGGTCAAGTTCAGATCCTCGACTGAAGATAAACATGAAAACAATACAAGCGCTGTC AAGGTCAGATGGTCAAATGAAGAACGTGAGAATGAGCTTGAACCGAAGGAGCAGCTTGT AGCTGAGGATGCAACATTGAATAATGGTTGGAAACAAGAAGAATACATTGGTAAAAAGG GAGGGAATGCTAGAAGATACACCTTAACCACCATTCAAGACATTCCAACTTCAAGC TGA MASDQIMHAQPGVSAIDGSNNYLNGQLWKLCAGPLFDTPKIGEKVETKTDEIYAEVSLL PDISDIEIPIPKNENTIQNINYFTKVLSVSDTQKSGGFVLYKRHAIECLPLLDMSQPTP NQDI IAKDIHGQEWSFKHTLRGTPKRHIFASGWNEFAKQKHLVAGDSFVFLRGENGESR VGISKAAHQQRNIVTSLMSKQSMHHGWATALNAIKNKCMFWFYKPRSSQFLVNINKF 4 S AA VDWNKKFSRGSKFSMKFEGKNLNEIRYNGTIVGVRDFSTHWKDSEWRSLEVQWDEAAT 2 1 IPRPDKVSPWEIELLTHSSNIFESDYLKHKRQIKVHEFGSKMGAPILSQGPENGQSTIQ SSMRYSFPTMSKPNYNEKMVQAMKETSTTTASTSCRLFGVNLTVPTTAKDPTEPIDTYK KLKMSNIFEEEKADHVQARIHTKVHMEGDVILIEPENNANMVKFRSSTEDKHENNTSAV KVRWSNEERENELEPKEQLVAEDATLNNGWKQEEYIGKKGGNARRYTLTTIQDIPTSS

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame is Sar_R_5279.gl9199.tl (SEQ ID NO:22), and encodes a TPR3 transcription factor (SEQ ID NO:23) to provide the auxin herbicide tolerance. See Table 11. In another aspect, the nucleic acid of SEQ ID NO:22 that encodes the polypeptide of SEQ ID NO:23 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_4l 12.gl0923.tl (SEQ ID NO:24), and encodes a TPR3 transcription factor (SEQ ID NO:25). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. GAGTCTAACCCGTGCATCGCTCTCTCTAAGAACGACTCATATGTCATGTCGGCTGCTGG AGGAAAAGTCTCGTTGTTCAACATGATGACTTTTAAGGTGATGACAACATTCATGCAAC CTCCACCGGCTTCAACATTTTTGGCGTTCCATCCTCAGGACAACAACATCATTGCCATT GGAATGGAGGACTCCACGATTCACATCTACAATGTCC GAGTGGATGAGGTCAAATCAAA GCTAAAGGGTCACCAGAAACGCATCACTGGCTTAGCATTTTCTACAACACTCAATATCT TGGTTTCATCTGGTGCTGATGCACAGATATGCTTTTGGAGCATTGACACATGGGAGAAG AGAAAATCCGTTGCAATACAAATGCCAGCAGGAAAAGCCGCCAATGGAGACACGCGTGT ACAGTTCCATGTGGATCAGATCCGTATCCTCGTAGTCCACGAGACACAACTAGCGATAT TCGATGCTTCCAAGATGGAATGTATCCGACAGTGGATTCCTCAAGACTCGTTGTCTGCT CCTATATCTTCAGCAGTGTATGCCTGCAACAGCCAGTTGATCTACACCACTTTCCGCGA TGGTAACATCGGAGTGTTCGACGCAGACACTCTTAGATTAAGATGTCGTATCTCTCCAT CCGCCTATTTGCCTCAAGGGAACCAAGGCTTGTCCCCTCTAGTTGTGGCGGCTCACCCG CAAGAGCCAAACCAGTTTGCGGTCGGTTTGAATGATGGGTCAGTTAAGGTGATAGAACC GACCGAGGCTGAAGGAAAGTGGGGGATGGTTCCACCTTCCGAAGCCTTCAACACTTCGC CATCCACCACAAACAACCAAACTCAAGAACACTTACAAAGATGA MSSLSRELVFLILQFLEEEKFKESVHRLEKESGFFFNTKYFDEKVLAGEWDEVEKYLSG FTKVDDNRYSMKIFFEIRKQKYLEALDRQDKAKAVEILVQDLRVFSTFNEDLYKEITQL LTLQNFRENDQLSKYGDTKTARTVMLAEVKKLIEANPLFRDKLTFPTLRSSRLRTLINQ SLNWQHQLCKNPRPNPDIKTLFTDHTCAVPNGPLAPSSVNQPVTTLTKPTAFPSLGAHG PFPPGAAVAAAANAGALASWMAAASGASAVQAAWT PAPMPLPQNQVAI LKRPRT PPAT PGIVDYQNPDHELMKRLRPAPSVEEVTYPAPRQQAPWSPEDLPLKVALALHQGSTVTSI EFHPMQNTLLLVGSATGEITLWELAVREKLVSRPFKVWDMTNCTNQFQALAAKETPISV TRVAWSPDGNFIGVAYSKHLVHLYAFSGPNDLRQHAEIDAHVGAVNDLAFAIPNRQLCV VTCGDDKLIKVWDVQGRKHFTFEGHDTPVYSICPHHKENIQFIFSTAIDGKIKAWLYDN RAA MGSRVDYDAPGKWCTTMLYSADGSRLFSCGTSKDGDFFLVEWNESEGSIKRTYLGFHKK 23 LAGWQFDTSKNHFLAVGEDGQIKFWDMDNINVLTSSDAEGGLPALPRLRFNREGNLLA VSTADNGFKILANTAGFRSLRAMEASAFETMRNPVDSSLTKAVPGAPVASGSCKIERGS PVRPSPILNGIDPSKPRIDDSTDKPRPWQLAEIMDPAQCRQATLPDTAGSSTKWRLLY TNSGAGMLALGLNGIQRLWKWVRSEQNPSGKATTAVAPQHWQPNSGLVMANDVSGVNLE ESNPCIALSKNDSYVMSAAGGKVSLFNMMTFKVMTTFMQPPPASTFLAFHPQDNNI A GMEDSTIHIYNVRVDEVKSKLKGHQKRITGLAFSTTLNILVSSGADAQICFWSIDTWEK RKSVAIQMPAGKAANGDTRVQFHVDQIRILWHETQLAIFDASKMECIRQWIPQDSLSA PISSAVYACNSQLIYTTFRDGNIGVFDADTLRLRCRISPSAYLPQGNQGLSPLWAAHP QEPNQFAVGLNDGSVKVIEPTEAEGKWGMVPPSEAFNTSPSTTNNQTQEHLQR Sar S 4112.gl0923.tl : TPR3 transcription factor ATGTCGTCGTTAAGCAGAGAGCTGGTGTTTCTCATACTTCAGTTTCTCGAGGAAGAGAA ATTCAAAGAGTCTGTTCACAGGCTGGAGAAAGAGTCGGGATTCTACTTTAATACGAAGT ATTTCGACGAGAAAGTGCTGGCTGGAGAGTGGGATGAGGTTGAGAAGTACTTGTCTGGG TTCACCAAGCTTGATGATAACAGATACTCCATGAAGATTTTCTTTGAAATTAGGAAGCA GAAGTATCTCGAGGCTCTTGATAAGCAAGATAAGGCCAAAGCAGTTGAGATATTGGTGC AGGACTTGAGAGTCTTCTCCACTTTCAACGAGGAGCTCTACAAAGAGATTACTCAACTT TTGACTTTGCACAACTTCAGGGAAAATGAGCAGCTTTCCAAATACGGAGACACTAAAAC TGCTCGGACCATAATGTTAGGCGAACTAAAGAAACTAATTGAAGCAAATCCTCTATTTC GTGACAAATTGACGTTCCCTACTTTGAGATCTTCGAGACTGCGGACTCTGATTAATCAA AGCCTTAATTGGCAGCACCAGCTGTGCAAGAATCCTAGGCCAAACCCAGATATTAAAAC SNT TCTATTCACAGACCACACATGCGCAGTTCCCAATGGTCCTCTGGCACCTTCACCAGTTA 24 ATCAGCCAGTTACGACCTTGACAAAGCCAACAGCTTTTCCGTCGCTTGGAGCTCATGGT CCCTTTCCTCCTCCTGGTGCTGCTGTTGCTGCTGCTGCTAATGCTGGCGCTTTAGCTAG TTGGATGGCTGCTGCCTCTGGTGCTTCTACTGTCCAAGCTGCCGTTGTCACTCCTGCGT CTATTCCCTTGCCTCAGAATCAAGTGTCAATCTTGAAGCGACCAAGAACACCACCAGCA ACTCCAGGTGTAGTAGATTATCAGAATCCTGATCATGAACTAATGAAGCGTCTCCGCCC TGCCCCATCTGTAGAGGAGGTGACATATCCTGCACCTAGGCAGCATGCTCCATTGTCGC TGGAAGACTTGCCGTTAAAGGCGGCTCTAGCGTTGCATCAAGGGTCCACTGTGACTAGC ATGGAGTTTCACCCTATGCAGAATACGTTACTTCTTGTCGGATCTGCCACCGGAGAAAT CACTTTATGGGAACTAGCTGTTCGAGAGAAGCTGGTTTCAAGGCCATTCAAAATATGGG ATATGGCTAATTGCTCACTTCCACTTCAGGCTTTGATAGCTAAAGAAACAGCAATGTCT GTCACCCGCGTTGCATGGAGTCCTGATGGAAATTTCATTGGGGTTGCGTATACGAAACA TGTTATTCACTTGTATGCTTTCTCTGGACCTAACGACCTTCGCCAGCATGCTGAGATTG ATGCCCATGTGGGTGCTGTGAACGACTTGGCTTTCGCTAATCCAAACAGACAGTTGTGT GTAGTTACTTGCGGAGATGATAAGCTGATCAAGGTATGGGACGTTTCAGGTCGGAAGCA TTTTACCTTTGAAGGTCACGAGGCTCCTGTTTATTCCATTTGCCCTCATCACAAAGAGA ACATTCAGTTCATATTTTCAACGGCCATAGATGGGAAAATAAAAGCCTGGCTTTATGAT AACATGGGTTCCAGAGTTGACTATGATGCTCCCGGTAAATGGTGTACTACAATGCTTTA TAGCGCTGATGGGACCAGATTGTTTTCTTGTGGAACGAGTAAAGATGGAGATTTTCACC TAGTTGAGTGGAACGAAAGTGAAGGATCAATCAAAAGGACCTATCTTGGGTTTCAGAAA AAGTTGGCAGGTATGGTTCAGTTTGATACCTCAAAGAACCACTTTCTGGCTGTTGGCGA GGATGGGCAAATCAAGTTCTGGGATATGGACAACATCAATGTTCTTACCAGCACTGATG CTGAGGGTGGACTTCCGGCTCTTCCTCGTTTGAGATTTAACAGGGAAGGAAATCTTCTA GCCGTTACTACGGCAGACAACGGATTTAAAGTCCTAGCAAACCAAGCTGGTTTCAGATC TCTGAGAGCCATGGAAACTTCAGCTTTTGAAAAGATGAGGACTCCAGCCGATTCTTCTT TAAGTAAAGCTGTAATGTTTCCCAACTTTGTGGTTGCTGGTGCTCCTGTTGCATCTGTC AGCTGTAAAGTTGAACGAGGCTCTCCTGCTAGACCTTCACCAATGCTGAATGGAGCTGA TCTCCCAAAGCCAAGAATCGATGACTCAACAGACAAACCAAAACCTTGGCTGTTAGCTG AAATCTTGGACCCTGCCCAGTGTCGTCAGGCCACTTTACCTGATACCACTGGTTCTTCC ACAAAGGTGGTTCGGCTTCTGTATACGAATTCCGGCGCTGGAATCTTGGCACTTGGTTT CAACGGTATTCAGAGGCTCTGGAAGTGGGTTCGCAATGAGCAAAACCCCAGTGGAAAGG CAACTGCCGCTGCTGTTCCTCAGCATTGGCAACCAAACAGTGGTCTTCTCATGACCAAC GATGTCTCTGGTGTAAACCTTGAAGAGGCCAACCCGTGCATCGCTCTCTCTAAGAACGA CTCATATGTCATGTCGGCTGCTGGAGGAAAAGTTTCGTTGTTCAACATGATGACTTTTA AGGTGATGACAACATTCATGCCACCTCCACCGGCATCAACATTTTTGGCGTTCCATCCT CAGGACAACAACATCATTGCCATTGGAATGGAGGACTCCACGATTCACATCTACAATGT CCGAGTGGATGAGGTCAAATCAAAGCTAAAGGGTCACCAGAAACGCATCACTGGCTTAG CATTTTCCACAGCCCTCAATATCTTGGTTTCATCTGGTGCTGATGCTCAGATATGCTTT TGGAGCATTGACACATGGGAGAAGAGAAAATCCGTTGCAATACAAATGCCAGCAGGAAA AGCCGCCAACGGAGACACGCGTGTTCAGTTTCATGTGGATCAGATCCGTATCCTCGCAG TCCACGAGACACAACTCGCGATATTTGATGCTTCCAAGATGGAATGTATTCGACAGTGG ATTCCTCAAGACTCGTTGGCTTCTCCTATATCTTCAGCAGTGTATGCATGTAACAGCCA GTTGATCTACACCACTTTCCGTGATGGTAACATTGGAGTGTTTGACGCAGACACTCTTA GATTAAGATGCCGTATCTCTCCATCCGCCTACTTGCCTCAAGGGAACCAAGGTTTGTCT CCTCTAGTTGTGGCAGCTCACCCGCAAGAGCCAAACCAGTTTGCGGTTGGTTTGAATGA CGGGTCAGTTAAGGTGATAGAACCGACCGAGGCTGAAGGCAAGTGGGGGATGGTTCCAC CCTCTGAAGCCATCACCACTTCACCATCCACTACAAGCAACCAAACTCCAGAACAGTTG CAAAGATGA MSSLSRELVFLILQFLEEEKFKESVHRLEKESGFYFNTKYFDEKVLAGEWDEVEKYLSG FTKLDDNRYSMKIFFEIRKQKYLEALDKQDKAKAVEILVQDLRVFSTFNEELYKEITQL LTLHNFRENEQLSKYGDTKTARTIMLGELKKLIEANPLFRDKLTFPTLRSSRLRTLINQ SLNWQHQLCKNPRPNPDIKTLFTDHTCAVPNGPLAPSPVNQPVTTLTKPTAFPSLGAHG PFPPPGAAVAAAANAGALASWMAAASGAS TVQAAWT PAS IPLPQNQVS ILKRPRT PPA TPGWDYQNPDHELMKRLRPAPSVEEVTYPAPRQHAPLSLEDLPLKAALALHQGSTVTS MEFHPMQNTLLLVGSATGEITLWELAVREKLVSRPFKIWDMANCSLPLQALIAKETAMS VTRVAWSPDGNFIGVAYTKHVIHLYAFSGPNDLRQHAEIDAHVGAVNDLAFANPNRQLC WTCGDDKLIKVWDVSGRKHFTFEGHEAPVYSICPHHKENIQFIFSTAIDGKIKAWLYD NMGSRVDYDAPGKWCTTMLYSADGTRLFSCGTSKDGDFHLVEWNESEGSIKRTYLGFQK SAA KLAGMVQFDTSKNHFLAVGEDGQIKFWDMDNINVLTSTDAEGGLPALPRLRFNREGNLL 25 AVTTADNGFKVLANQAGFRS LRAMETSAFEKMRT PADSSLSKAVMFPNFWAGAPVAS V SCKVERGSPARPSPMLNGADLPKPRIDDSTDKPKPWLLAEILDPAQCRQATLPDTTGSS TKWRLLYTNSGAGILALGFNGIQRLWKWVRNEQNPSGKATAAAVPQHWQPNSGLLMTN DVSGVNLEEANPCIALSKNDSYVMSAAGGKVSLFNMMTFKVMTTFMPPPPASTFLAFHP QDNNI IAIGMEDSTIHIYNVRVDEVKSKLKGHQKRITGLAFSTALNILVSSGADAQICF WSIDTWEKRKSVAIQMPAGKAANGDTRVQFHVDQIRILAVHETQLAIFDASKMECIRQW IPQDSLASPISSAVYACNSQLIYTTFRDGNIGVFDADTLRLRCRISPSAYLPQGNQGLS PLWAAHPQEPNQFAVGLNDGSVKVIEPTEAEGKWGMVPPSEAITTSPSTTSNQTPEQL QR Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame S r R l 119.g4334.tl (SEQ ID NO:26), and encodes a Chloroplast Trans- Membrane, Auxin-Associated Protein- 1 (cpTAAP-1) (SEQ ID NO:27) to provide the auxin herbicide tolerance. See Table 12. In another aspect, the nucleic acid of SEQ ID NO:26 that encodes the polypeptide of SEQ ID NO:27 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_31217.g55588.tl (SEQ ID NO:28), and encodes a Chloroplast Trans-Membrane, Auxin-Associated Protein- 1 (cpTAAP-1) (SEQ ID NO:29). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. ASLVAQRSLDFVENCDLPTPQKMNRSYYGSPRSFDSDHSFSNQTTHVTRRGNSFKNRTE EASSESELSKSELLEALRRSQTRAREAENMAKEACAEKEHLVKLLLKQASELFGYKQLL QLLQLESLYLQIKSKKIEDSKETPPASIPWSNTKGRKGGRKRRSKRSKPNGFVGLALGM SLVGAGLLLGWTVGWMQMLSF Sar_S_31217.g55588.tl: Chloroplast Trans-Membrane, Auxin-Associated Protein-1 (cpTAAP-1) ATGATGGCAGCAGGAGAAGCAAGAGCTGTGTGGCAAAGAACAGTAAACCGTTACTTTGT CCAAGAAGACGCTAAAAGAGCTCCCAAGTTAACCTCCTCTTGCCAATCTTCTTCTTCCT CAACAGCTTCCACTAAACAGGTTGAAGATTCTCGTCCTGTTGTTGATCCACAGAACCAA TCACCATCTTGTGCAGGTTTCATGCCTCTTCAACCAAACCCCAGTTTCCCTCATCTGTC ACCACAGAGAACCAGTTTGTGGGGCCATCATCATCACATTCAGCAAGACCACAAGGAAC AAGTGAAGACGCCATTGGAAGCTGAGTCCGAGGGAGCCAAGTCTGAAAGGTTTCAAGAG TTTATAGAGTTGATGGAGACAAGGGAGAGTTATGGTTCGGTTGGGAATGATGAGTCCTC TGAGAAAAAGTTAGCAAGTGAGCTAGCTTTTGATCCCAGCTCTCCTTGGAATCCTCTCT CTAGCGAGAAAGCTGCACCCTGGTGGAGAACTACAGACAAAGATGAACTAGCTTCCTTG 6 SNT GTTGCACAAAGGTCTCTAGACTTTGTTGAGAACTGTGACCTGCCAACGCCGCAGAAGAT 28 GAACCGCTCTTACTATGGTAGTCCACGCTCTTTCGACTCTGATCACTCTTTCTCTAACC AAACCACCCACGTGCCAAGGAGAGGAAACAGCTTCAAGAACAGAACAGAGGAAGCTTCC TCTGAGTCTGAACTGAGCAAATCCGAGCTGCTTGAAGCGCTGAGGCGTTCTCAAACGCG AGCTAGGGAAGCTGAAAACATGGCAAAAGAAGCGTGCGCAGAGAAAGAGCATTTGGTGA AGCTCTTGTTAAAGCAAGCCTCAGAGCTTTTTGGGTATAAGCAGTTGCTGCAGCTGCTC CAGCTCGAATCACTTTACCTCCAAATCCAGAACAAGAAGATCGAGGACAGCAAGGAGAC ACCGCCGGCTTCCATCCCTTGGAGCAACACCAAAGGAAGAAAAGGAGGGAGAAAGAGAA GAAGCAAAAGGAGTAAACCGAATGGGTTTGTTGGTTTAGCATTGGGGATGAGTCTGGTT GGTGCTGGTTTGCTTCTGGGATGGACTGTTGGATGGATGCAGATGCTTTCTTTCTAA MMAAGEARAVWQRTVNRYFVQEDAKRAPKLTSSCQSSSSSTASTKQVEDSRPWDPQNQ SPSCAGFMPLQPNPSFPHLSPQRTSLWGHHHHIQQDHKEQVKTPLEAESEGAKSERFQE FIELMETRESYGSVGNDESSEKKLASELAFDPSSPWNPLSSEKAAPWWRTTDKDELASL 6 S AA VAQRSLDFVENCDLPTPQKMNRSYYGSPRSFDSDHSFSNQTTHVPRRGNSFKNRTEEAS 29 SESELSKSELLEALRRSQTRAREAENMAKEACAEKEHLVKLLLKQASELFGYKQLLQLL QLESLYLQIQNKKIEDSKETPPASIPWSNTKGRKGGRKRRSKRSKPNGFVGLALGMSLV GAGLLLGWTVGWMQMLSF

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame 5ar_R_21396.g52442.tl (SEQ ID NO:30), and encodes an Endo-Mitochondrial, Auxin-Associated Protein-1 (mtAAP-1) (SEQ ID NO:31) to provide the auxin herbicide tolerance. See Table 13. In another aspect, the nucleic acid of SEQ ID NO:30 that encodes the polypeptide of SEQ ID NO:31 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_20600.g42686.tl (SEQ ID NO:32), and encodes an Endo-Mitochondrial, Auxin-Associated Protein-1 (mtAAP-1) (SEQ ID NO:33). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide.

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame S r_R_3394.gl2813.tl (SEQ ID NO:34), and encodes a zinc-binding ribosomal protein family protein (SEQ ID NO:35) to provide the auxin herbicide tolerance. See Table 14. In another aspect, the nucleic acid of SEQ ID NO:34 that encodes the polypeptide of SEQ ID NO:35 provides the DART auxinic herbicide tolerance trait to a plant. No wild type auxin herbicide susceptible counterpart was identified.

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame Sar_R_4824.gl7686.tl (SEQ ID NO:36), and encodes a SAUR-like auxin- responsive protein family transcription factor (SEQ ID NO:3 ) to provide the auxin herbicide tolerance. See Table 15. In another aspect, the nucleic acid of SEQ ID NO:36 that encodes the polypeptide of SEQ ID NO:37 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises 5ar_S_12132.g28371.tl (SEQ ID NO:38), and encodes a SAUR-like auxin-responsive protein family transcription factor (SEQ ID NO:39). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. AGCTTCGATTTCGGCGACTGCGTCGAGGAATTTCTTTGA MMMRKRIASFKNLAKRVNS INTKEGGSES PLIGEADDS S STAWAAAKTPTGSCAVYVG 9 RAA EERVRRWPTSYLSHPLFRMLLDKSYDELHCFEQKVMLWPCSLSVFQDWNAIESCNG 37 SFDFGDCVEEFL Sar_S_12132.g28371.tl: SAUR-like auxin-responsive protein family transcription factor ATGATGATGAGGAAAAGAATAGC TTCCTTCAAGAAT CTAGCCAAGAGAGTGAACAGCAT TAACACGAAAGAAGGCGGCTCCGAGTCTCCTCTGATCGGTGATGCGGACGACTCGTCTT CGACAGCGGTGGTGGCGGCGGCTAAGACGCCTACGGGATCTTGTGCGGTGTACGTAGGG 9 S NT GAGGAGCGCGTGAGACGCGTGGTGCCGACAAGCTATTTGAGTCATCCTCTCTTCAGGAT 38 GTTACTAGACAAGTCATACGACGAGTTGCACTGTTTCGAGCAGAAGGTTATGTTGGTTG TCCCTTGTAGCCTGTCCGTTTTTCAAGACGTCGTCAACGCCATCGAGTCTTGTAACGGC AGCTTCGATTTCGGCGACTGTGTCGAGGAATTTCTTTGA MMMRKR AS FKN LAKRVN S TKEGGSE S P L GDADDS S S TAWAAAKT P TGSCAVYVG 9 S AA EERVRRWPTSYLSHPLFRMLLDKSYDELHCFEQKVMLWPCSLSVFQDWNAIESCNG 39 SFDFGDCVEEFL

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame ar_R_38470.g64923.t (SEQ ID NO:40), and encodes a HTA13, histone protein (SEQ ID NO:41) to provide the auxin herbicide tolerance. See Table 16. In another aspect, the nucleic acid of SEQ ID NO:40 that encodes the polypeptide of SEQ ID NO:41 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_4108.gl 0914.t l (SEQ ID NO:42), and encodes a HTA13, histone protein (SEQ ID NO:43). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. Sar S 4108.gl0914.tl: HTA13, histone ATGACGGGTCGTGGAAAAACTCTCGGATCTGGGGTTGCTAAGAAGGCGACGTCTCGGAG CAGCAAGGCGGGTCTCCAGTTCCCCGTCGGTCGTATCGCCCGGTTTCTGAAGAACGGCA AGTACGCCGAACGTGTTGGCGCCGGAGCTCCGGTTTACTTGGCCGCCGTACTCGAATAC 10 S NT CTCGCCGCAGAGGTTTTGGAATTGGCTGGAAACGCGGCGAGGGACAACAAGAAGACGAG 42 AATCGTGCCGCGTCACATTCAATTGGCGGTGAGGAACGACGAGGAGCTGAGTAAGTTGC TTGGAGACGTGACGATTGCTAATGGAGGTGTGATGCCGAACATTCACAACCTTCTTCTT CCCAAGAAGGCTGGTGGGGCTTCCAAACCTTCCGGTGATGACGAATAG MTGRGKTLGSGVAKKATSRSSKAGLQFPVGRIARFLKNGKYAERVGAGAPVYLAAVLEY 10 S AA LAAEVLELAGNAARDNKKTRIVPRHIQLAVRNDEELSKLLGDVTIANGGVMPNIHNLLL 43 PKKAGGASKPSGDDE

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame Sar_R_706.G2840.Tl (SEQ ID NO:44), and encodes a knotted 1 like (KNAT4) transcription factor (SEQ ID NO:45) to provide the auxin herbicide tolerance. See Table 17. In another aspect, the nucleic acid of SEQ ID NO:44 that encodes the polypeptide of SEQ ID NO:45 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_5265.G13674.Tl (SEQ ID NO:46), and encodes a knotted 1 like (KNAT4) transcription factor (SEQ ID NO:47). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. ATAAGAGAGGAGATATTGAGAAAGAGAAGAGCTGGGAAATTACCAGGAGACACCACCTC GGTTCTCAAAGCTTGGTGGCAGTCTCATTCTAAGTGGCCTTACCCTACTGAGGAAGATA AGGCGAGGTTGGTGCAGGAGACGGGTTTGCAGCTCAAACAGATAAACAATTGGTTCATC AATCAAAGAAAGAGGAATTGGCATAGTAATCCATCTTCTTCCACCGCCTCAAAGAACAA ACGCAAAAGATTAAGAGGGACTAGGGCTAATAGTGGCAATTCAGTAGTAGCAGAAGGGC GTCTCCTT AC TTAACTTTAAGAGACAAAAAGACACAGAGGAATAA MAFHHNHFNHFTDQQHQPPPLPPPQTQQEHHFHESTPPNWLLRSDNNFLNLHTPASAAA TSSDSPSSAAANQWLSRSSSFLQRGGGASNNNAGSGDAMENVTGGGEESMIGENARHKA EILSHPLYEQLLSAHVACLRIATPVDQLPRIDAQLAQSQNWAKYSTLDAAAQGLIPGD 1 1 RAA DKELDHFMTHYVLLLCSFKEQLQQHVRVHAMEAVMACWEIEQSLQSFTGVSPGEGTGAT 45 MSEDEDEQVESDAHLFDGSLDGLGFGPLVPTESERSLMERVRQELKHELKQGYKEKIVD IREEILRKRRAGKLPGDTTSVLKAWWQSHSKWPYPTEEDKARLVQETGLQLKQINNWFI NQRKRNWHSNPSSSTASKNKRKRLRGTRANSGNSWAEGRLLYFNFKRQKDTEE Sar S 5265.G13674.T1: Knotted 1 like (KNAT4) transcription factor ATGGCGTTTCATCACAATCATTTCAATCACTTCACCGACCAACAACACCAGCCTCCTCC TCTTCCTCCGCCACAGACGCAGCAGGAACACCATTTCCACGAATCCACACCGCCTAACT GGCTCCTCCGCTCCGACAACAACTTTCTAAACCTCCACACTCCCGCCTCCGCCGCCGCT ACAAGCTCAGATTCTCCATCCTCCGCCGCCGCTAACCAGTGGCTCTCTCGTTCCTCCTC CTTCCTCCAACGCGGCGGCGGCGCCAGCAACAATAACGCCGGCTCTGGTGACGCCATGG AAAACGTTACCGGTGGAGGAGAGGAGTCGATGATCGGCGAGAATGCGAGGCAAAAGGCG GAGATACTGTCTCATCCACTATACGAGCAGCTGTTGTCGGCACACGTGGCGTGCCTGAG GATCGCGACGCCGGTGGATCAGCTTCCGAGGATCGACGCACAGCTTGCTCAGTCGCAGA ACGTCGTGGCTAAGTACTCAACGTTAGACGCTGCTGCTCAAGGACTCATCCCCGGAGAT GATAAGGAGCTTGACCACTTCATGACGCATTATGTACTATTGCTTTGCTCTTTTAAAGA 1 1 SNT ACAGTTGCAACAGCATGTTCGTGTCCATGCAATGGAAGCTGTTATGGCTTGTTGGGAGA 46 TTGAGCAGTCTCTTCAAAGCTTCACTGGAGTGTCTCCTGGTGAAGGCACAGGAGCAACA ATGTCTGAAGACGAAGATGAGCAAGTAGAGAGTGACGCTCATTTGTTTGATGGAAGCTT AGATGGGTTAGGGTTTGGTCCTCTTGTCCCCACTGAGAGTGAGAGATCCTTGATGGAAC GAGTTAGACAAGAACTCAAACATGAACTCAAGCAGGGCTACAAGGAGAAAATTGTAGAC ATAAGAGAGGAGATATTGAGAAAGAGAAGAGCTGGGAAATTACCAGGAGACACCACCTC GGTTCTCAAAGCTTGGTGGCAGTCTCATTCTAAGTGGCCTTACCCTACTGAGGAAGATA AGGCGAGGTTGGTGCAGGAGACGGGTTTGCAGCTCAAACAGATAAACAATTGGTTCATC AATCAAAGAAAGAGGAATTGGCATAGTAATCCATCTTCTTCCACCGCCTCAAAGAACAA ACGCAAAAGATTAAGAGGGACTAGGGCTAATAGTGGCAATTCAGTAGTAGCAGAAGGGC GTCTCCTTTACTTTAACTTTAAGAGACAAAAAGACACAGAGGAATAA MAFHHNHFNHFTDQQHQPPPLPPPQTQQEHHFHESTPPNWLLRSDNNFLNLHTPASAAA TSSDSPSSAAANQWLSRSSSFLQRGGGASNNNAGSGDAMENVTGGGEESMIGENARQKA EILSHPLYEQLLSAHVACLRIATPVDQLPRIDAQLAQSQNWAKYSTLDAAAQGLIPGD 11S AA DKELDHFMTHYVLLLCSFKEQLQQHVRVHAMEAVMACWEIEQSLQSFTGVSPGEGTGAT 47 MSEDEDEQVESDAHLFDGSLDGLGFGPLVPTESERSLMERVRQELKHELKQGYKEKIVD IREEILRKRRAGKLPGDTTSVLKAWWQSHSKWPYPTEEDKARLVQETGLQLKQINNWFI NQRKRNWHSNPSSSTASKNKRKRLRGTRANSGNSWAEGRLLYFNFKRQKDTEE

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame Sar_R_19858.g50467.tl (SEQ ID NO:48), and encodes a Zinc-Finger-Like, Auxin-Associated Protein-1 (zFAAP-1) (SEQ ID NO:49) to provide the auxin herbicide tolerance. See Table 18. In another aspect, the nucleic acid of SEQ ID NO:48 that encodes the polypeptide of SEQ ID NO:49 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises 5ar_S_68453.g74218.tl (SEQ ID NO:50), and encodes a Zinc-Finger-Like, Auxin-Associated Protein-1 (zFAAP-1) (SEQ ID NO:51). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide.

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame Sar_R_10390.g33598.tl (SEQ ID NO:52), and encodes an Alba DNA/RNA- binding protein transcription factor (SEQ ID NO:53) to provide the auxin herbicide tolerance. See Table 19. In another aspect, the nucleic acid of SEQ ID NO:52 that encodes the polypeptide of SEQ ID NO:53 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_2341 l.g46486.tl (SEQ ID NO:54), and encodes an Alba DNA/RNA-binding protein transcription factor (SEQ ID NO:55). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. GAGGAAGAGGAAGGGGACGAGGTGGCAGAGATGGAGGTTGGGAAGGTAACCAGTCGGAT GGTAGAGGAAGAGGGCGTGGCAGAGGACGCAGTAGTCGTGGTCGAGGAAGAGGAGGATA CAGTGGTCCTCCAAATGAGTATGATGCACCACAAGATAGAGGTTACGGTTACGATGCCC CTCATGAACACCGTGGATATGATGATCATGGTGGTTATGATGGTCCTCGTCAGGGCCGC GGTGGTTACAATGGTCCTCTGCGTGGACGTGGAAGGGGAGGTCGTGGAAGAGGACGAGG TGGTCGTGGTATTGGTGGTGGATTCAACAACAGCTCAGATGGACCAACAAACCAGGCAG TCGCTTTAGACATATGA MEKYDRWKKKEERT DANE R SMGRARNY YAMTLLQEKGS TEWFKAMGRAINK TV VEL KRR PGLHQNTS GS D TDTWEPKEEGLLPIETTRHVSMI T LSTKELN 13 S AA TS SVGYQCPI PIEMVKPLGDTDYEGGAEGPPGGRGRGRGRGRGRGRGGRDGGWEGNQSD 55 GRGRGRGRGRS SRGRGRGGYSGPPNEYDAPQDRGYGYDAPHEHRGYDDHGGYDGPRQGR GGYNGPLRGRGRGGRGRGRGGRG GGGFNNS SDGPTNQAVALD

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame 5ar_R_241 1.G9430.Tl (SEQ ID NO:56), and encodes an IAA16 transcription factor (SEQ ID NO:57) to provide the auxin herbicide tolerance. See Table 20. In another aspect, the nucleic acid of SEQ ID NO:56 that encodes the polypeptide of SEQ ID NO:57 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises 5ar_S_29291.G53554.Tl (SEQ ID NO:58), and encodes an IAA16 transcription factor (SEQ ID NO:59). In another aspect, a second auxin herbicide susceptible nucleic acid open reading frame is 5ar_S_3202.G8615.Tl (SEQ ID NO:60), and encodes an IAA16 transcription factor (SEQ ID NO:61). In another aspect, either of the auxin herbicide susceptible nucleic acids is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. TCTCTTTGTGGATCACTCCTGTCGCTTACCAAATGATGCACGAGCACCATCCCCTGTCA ACAATCCGCTGCTTGGATCACTACCAAAAGCCGGGGGATTTCCTCCTTTAGGTGCACAT GGGCCATTTCAACCAACACCTTCTCAGGTTCCGACACCTCTTGCTGGTTGGATGTCTAG TTCTTCCTCTGTTCCTCATCCAGCTGTGTCTGGAGGAGCCATAGCTCTAGGTGCTCCAT CCATCCAAGCCTTGAAGCACCCGAGAACTCCTCCAAGTAATTCCGCTGTAGACTATCCA TCAGGTGATTCGGACCATGTCTCAAAAAGAACAAGACCTATGGGAATCTCTGACGAGGT GAATCTTGGTGTGAACATGTTACCGATGACATTCCCGGGACAGGCACATGGTCATACCC AAGCCTTCAAAGCACCTGATGATTTGCCTAAGACAGTAGCACGAACTTTGAGCCAAGGC TCATCTCCCATGAGCATGGATTTCCATCCAATTAAACAGACTCTCCTACTAGTTGGTAC AAACGTTGGAGATATTGGACTCTGGGAAGTCGGTTCTCGAGAACGTCTAGTACAAAAGA CTTTCAAAGTTTGGGACTTGAGTAAATGTTCAATGCCCTTGCAGGCTGCTTTAGTGAAA GAACCTGTTGTTTCTGTCAACCGTGTGATTTGGAGCCCAGATGGTTCTTTATTCGGAGT TGCTTATTCGAGACATATTGTACAGCTATACTCTTATCACGGTGGTGAAGATATGAGAC AACACCTTGAGATTGATGCTCATGTTGGTGGTGTAAATGACATTGCATTCTCCACTCCA AACAAGCAACTATGTTTTACTACTTGTGGTGATGACAAAACCATCAAGGTCTGGGATGC TGCAACGGGTGTAAAAAGGCATACTTTCGAAGGCCATGAAGCTCCTGTTTACTCTATCT GCCCTCATTACAAGGAAAACATTCAGTTCATCTTTTCAACTGCGCTTGACGGGAAAATA AAAGCGTGGCTATATGATAATATGGGTTCTCGGGTTGACTACGAAGCTCCTGGTCGCTG GTGTACAACGATGGCCTACAGTGCTGATGGAACTCGGCTATTTTCTTGTGGGACGAGTA AAGATGGGGATTCATACATAGTTGAGTGGAATGAAAGCGAAGGAGCTGTTAAGAGAACT TATCAAGGATTCCACAAGCGTTCCCTTGGTGTCGTTCAGTTTGATACTACTAAAAACCG CTATCTTGCTGCTGGTGATGACTTCTCCATTAAGTTCTGGGATATGGACAATGTACAGC TGTTGACTGCCATTGATGCTGATGGTGGTCTTCAGGCAAGCCCACGGATCCGGTTTAAC AAGGAAGGCTCTCTCTTGGCCGTTTCTGCCAATGACAATATGATTAAGGTTATGGCAAA CACAGATGGTTTAAGGCTACTGCACACGGTCGACAACTTATCTTCTGAATCCTCCAAGC CTGCGATCAACAACATTGCAGTGGCAGAGAGAGCTGCTGAGAGACCTGCCTCTGTAGTC TCCATCCCTGGAATGAATGGAGATTCACGGAATATGGTAGATGTGAAGCCAGTGATCAC TGAAGAATCAAA GA AAG C AAGA A GGAAGCTTACTGAAGTCGGTGAACCCTCTC AATGCCGCTCGTTGAGACTCCCTGAGAATATGAGAGTCACCAAGATATCGAGATTGATC TTCACAAATTCTGGAAATGCGATACTGGCATTGGCATCAAACGCTATTCATCTGCTTTG GAAATGGCAGCGAAATGACCGTAACGCTACTGGAAAGGCAACGGCTTCTTTACCTCCTC AGCAGTGGCAACCAGCGAGTGGAATCTTAATGACAAACGATGTGACTGAAACTAATTCA GAGGAAGCTGTACCGTGTTTTGCTTTATCCAAGAATGATTCGTATGTGATGTCAGCATC TGGAGGAAAGATCTCTTTATTTAATATGATGACGTTTAAGACAATGGCTACTTTCATGC CGCCTCCGCCTGCAGCAACGTTTCTGGCTTTTCACCCTCAGGACAACAATATAATTGCA ATTGGAATGGATGATAGTACAATACAGATTTACAATGTTCGCGTTGATGAGGTCAAGAG CAAGCTTAAAGGACATTCCAAGAGAATAACCGGCCTCGCATTCTCCAACGTACTAAACG TTCTGGTTTCATCTGGAGCAGACGCTCAGATTTGTGTATGGAACACGGATGGATGGGAG AAGCAGAGAAGCAAGGTTCTGCAAGTTCCACAGGGAAGATCAACGGGTGCTCTTTCAGA CACACGCGTTCAGTTTCATCAAGATCAAGTACACTTCCTCGTGGTCCATGAAACTCAGC TCGCTATATACGAAACAACCAAGCTTGAATGCATGAAACAGTGGCCTGTGCGTGAATCA TCAGCTCCAATCACACATGCCACATTCTCATGTGATAGCCAGTTGATATACGCAAGTTT CATGGATGCTACAATCTGCGTCTTCACCTCTGCAAACCTTCGATTGCGTTGCAGAGTCA ATCCCTCTGCGTATTTGCCTGCTTCTCTCAGCAACTCGAACGTCCATCCGCTGGTGATT GCGGCTCATCCTCAAGAATCCAATATGTTTGCTGTGGGTCTCTCAGACGGTGGGGTCCA TATATTCGAGCCGCTCGAGTCAGAAGGTAAATGGGGAGTGGCTCCACCACCTGAGAACG GCTCATCCAGCGCTTTGGCGTCTGCACCTTCCGTTGGAGCTTCCGCATCCGATCAGCCT CAGAGATAA MSSLSRELVFLILQFLDEEKFKETVHKLEQESGFFFNMKYFEDEVHSGNWDEVEKYLSG FTKVDDNRYSMKIFFEIRKQKYLEALDKHDRPKAVEILVKDLRVFSTFNEELFKEITQL LTLENFRENEQLSKYGDTKSARSIMLVELKKLIEANPLFRDKLQFPTLRNSRLRTLINQ SLNWQHQLCKNPRPNPDIKTLFVDHSCRLPNDARAPSPVNNPLLGSLPKAGGFPPLGAH RAA GPFQPTPSQVPTPLAGWMSSSSSVPHPAVSGGAIALGAPSIQALKHPRTPPSNSAVDYP 57 SGDSDHVSKRTRPMGISDEVNLGVNMLPMTFPGQAHGHTQAFKAPDDLPKTVARTLSQG SSPMSMDFHPIKQTLLLVGTNVGDIGLWEVGSRERLVQKTFKVWDLSKCSMPLQAALVK EPWSVNRVIWSPDGSLFGVAYSRHIVQLYSYHGGEDMRQHLEIDAHVGGVNDIAFSTP NKQLCFTTCGDDKTIKVWDAATGVKRHTFEGHEAPVYSICPHYKENIQFIFSTALDGKI KAWLYDNMGSRVDYEAPGRWCTTMAYSADGTRLFSCGTSKDGDSYIVEWNESEGAVKRT YQGFHKRSLGWQFDTTKNRYLAAGDDFSIKFWDMDNVQLLTAIDADGGLQASPRIRFN KEGSLLAVSANDNMIKVMANTDGLRLLHTVDNLSSESSKPAINNIAVAERAAERPASW SIPGMNGDSRNMVDVKPVITEESNDKSKIWKLTEVGEPSQCRSLRLPENMRVTKISRLI FTNSGNAILALASNAIHLLWKWQRNDRNATGKATASLPPQQWQPASGILMTNDVTETNS EEAVPCFALSKNDSYVMSASGGKISLFNMMTFKTMATFMPPPPAATFLAFHPQDNNIIA IGMDDSTIQIYNVRVDEVKSKLKGHSKRITGLAFSNVLNVLVSSGADAQICVWNTDGWE KQRSKVLQVPQGRSTGALSDTRVQFHQDQVHFLWHETQLAIYETTKLECMKQWPVRES SAPITHATFSCDSQLIYASFMDATICVFTSANLRLRCRVNPSAYLPASLSNSNVHPLVI AAHPQESNMFAVGLSDGGVHIFEPLESEGKWGVAPPPENGSSSALASAPSVGASASDQP QR Sar S 29291.G53554.T1: IAA16 transcription factor ATGTCTTCTCTCAGTAGAGAGCTCGTGTTCTTGATCTTACAGTTTCTAGATGAAGAGAA GTTCAAAGAGACTGTTCATAAGCTGGAACAAGAGTCTGGGTTTTTCTTCAATATGAAGT ATTTTGAGGATGAAGTGCACAGTGGGAACTGGGATGAGGTGGAGAAGTATCTCTCTGGT TTTACCAAAGTTGATGATAATAGATACTCCATGAAGATATTCTTCGAGATTAGGAAGCA GAAGTATCTCGAGGCCTTGGATAAGCATGATCGTCCAAAGGCTGTTGAGATTTTAGTGA AAGATTTGAGAGTGTTTTCCACTTTTAATGAGGAGCTTTTCAAGGAAATCACTCAGCTC TTGACCTTAGAGAACTTTCGGGAGAACGAGCAGTTATCCAAGTACGGAGACACAAAGTC AGCCAGATCTATCATGTTGGTGGAACTTAAGAAGTTGATTGAAGCGAATCCATTATTCC GTGATAAGCTGCAGTTTCCTACCCTTAGAAACTCACGGCTGAGAACGCTGATTAACCAG AGCTTAAACTGGCAACATCAGCTTTGTAAAAACCCAAGGCCAAATCCTGATATCAAGAC TCTCTTTGTGGATCACTCCTGTCGCTTACCAAATGATGCACGAGCACCATCCCCTGTCA ACAATCCGCTGCTTGGATCACTACCAAAAGCCGGGGGATTTCCTCCTTTAGGTGCACAT GGGCCATTTCAACCAACACCTTCTCAGGTTCCGACACCTCTTGCTGGTTGGATGTCTAG TTCTTCCTCTGTTCCTCATCCAGCTGTGTCTGGAGGAGCCATAGCTCTAGGTGCTCCAT CCATCCAAGCCTTGAAGCACCCGAGAACTCCTCCAAGTAATTCCGCTGTAGACTATCCA TCAGGTGATTCGGACCATGTCTCAAAAAGAACAAGACCTATGGGAATCTCTGACGAGGT GAGTCTTGGCGTGAACATGTTACCGATGACATTCCCGGGACAGGCACATGGTCATACCC AAGCTTTCAAAGCACCTGATGACTTGCCTAAGACAGTAGCACGAACTTTGAGCCAAGGC TCTTCTCCCATGAGCATGGATTTCCATCCAATCAAACAGACTCTCCTACTCGTTGGTAC AAATGTTGGGGATATTGGGCTCTGGGAAGTCGGTTCTCGAGAACGTCTAGTACAAAAGA CTTTCAAAGTTTGGGACTTGAGTAAATGTTCAATGCCCTTGCAGGCTGCTTTAGTGAAA GAACCTGTTGTTTCTGTCAACCGTGTGATTTGGAGCCCAGATGGTTCTTTATTCGGAGT S NT TGCTTATTCGAGACATATTGTACAGCTATACTCTTATCACGGTGGTGAAGATATGAGAC 58 AACACCTTGAGATTGATGCTCATGTTGGTGGTGTAAATGACATTGCATTCTCCACTCCA AACAAGCAACTATGTTTTACTACTTGTGGTGATGACAAAACCATCAAGGTCTGGGATGC TGCAACGGGTGTAAAAAGGCATACTTTCGAAGGCCATGAAGCTCCTGTTTACTCTATCT GCCCTCATTACAAGGAAAACATTCAGTTCATCTTTTCAACTGCGCTTGACGGGAAAATA AAAGCGTGGCTATATGATAATATGGGTTCTCGGGTTGACTACGAAGCTCCTGGTCGCTG GTGTACAACGATGGCCTACAGTGCTGATGGAACTCGGCTATTTTCTTGTGGGACGAGTA AAGATGGGGATTCATACATAGTTGAGTGGAATGAAAGCGAAGGAGCTGTTAAGAGAACT TATCAAGGATTCCACAAGCGTTCTCTTGGTGTCGTTCAGTTTGATACTACTAAAAACCG CTATCTTGCTGCTGGTGATGACTTCTCCATTAAGTTCTGGGATATGGACAATGTACAGC TGTTGACTGCCATTGATGCTGATGGTGGTCTTCAGGCAAGCCCACGGATCCGGTTTAAC AAGGAAGGCTCTCTCTTGGCCGTTTCTGCCAATGACAATATGATTAAGGTTATGGCAAA CACAGATGGTTTAAGGCTACTGCACACGGTCGACAACTTATCTTCTGAATCCTCCAAGC CTGCGATCAACAACATTGCAGTGGCAGAGAGAGCTGCTGAGAGACCTGCCTCTGTAGTC TCCATCCCTGGAATGAATGGAGATTCACGGAATATGGTAGATGTGAAGCCAGTGATCAC TGAAGAATCAAATGATAAGTCTAAGATATGGAAGCTTACTGAAGTCGGTGAACCCTCTC AATGCCGCTCGTTGAGACTCCCTGAGAATATGAGAGTCACCAAGATATCGAGATTGATC TTCACAAATTCTGGAAATGCGATACTGGCATTGGCATCAAACGCTATTCATCTGCTTTG GAAATGGCAGCGAAATGACCGTAACGCTACTGGAAAGGCAACGGCTTCTTTACCTCCTC AGCAGTGGCAACCAGCGAGTGGAATCTTAATGACAAACGATGTGACTGAAACTAATTCA GAGGAAGCTGTACCGTGTTTTGCTTTATCCAAGAATGATTCGTATGTGATGTCAGCATC TGGAGGAAAGATCTCTTTATTTAATATGATGACGTTTAAGACAATGGCTACTTTCATGC CGCCTCCGCCTGCAGCAACGTTTCTGGCTTTTCACCCTCAGGACAACAATATAATTGCA ATTGGAATGGATGATAGTACAATACAGATTTACAATGTTCGCGTTGATGAGGTCAAGAG CAAGCTTAAAGGACATTCCAAGAGAATAACCGGCCTCGCATTCTCCAACGTACTAAACG TTCTGGTTTCATCTGGAGCAGACGCTCAGATTTGTGTATGGAACACGGATGGATGGGAG AAGCAGAGAAGCAAGGTTCTGCAAGTTCCACAGGGAAGATCAACGGGTGCTCTTTCAGA CACACGCGTTCAGTTTCATCAAGATCAAGTACACTTCCTCGTGGTCCATGAAACTCAGC TCGCTATATACGAAACAACCAAGCTTGAATGCATGAAACAGTGGCCTGTGCGTGAATCA TCAGCTCCAATCACACATGCCACATTCTCATGTGATAGCCAGTTGATATACGCAAGTTT CATGGATGCTACAATCTGCGTCTTCACCTCTGCAAACCTTCGATTGCGTTGCAGAGTCA ATCCCTCTGCGTATTTGCCTGCTTCTCTCAGCAACTCGAACGTCCATCCGCTGGTGATT GCGGCTCATCCTCAAGAATCCAATATGTTTGCTGTGGGTCTCTCAGACGGTGGGGTCCA TATATTCGAGCCGCTCGAGTCAGAAGGTAAATGGGGAGTGGCTCCACCACCTGAGAACG GCTCATCCAGCGCTTTGGCGTCTGCACCTTCCGTTGGAGCTTCCGCATCCGATCAGCCT CAGAGATAA MSSLSRELVFLILQFLDEEKFKETVHKLEQESGFFFNMKYFEDEVHSGNWDEVEKYLSG FTKVDDNRYSMKIFFEIRKQKYLEALDKHDRPKAVEILVKDLRVFSTFNEELFKEITQL LTLENFRENEQLSKYGDTKSARSIMLVELKKLIEANPLFRDKLQFPTLRNSRLRTLINQ SLNWQHQLCKNPRPNPDIKTLFVDHSCRLPNDARAPSPVNNPLLGSLPKAGGFPPLGAH GPFQPTPSQVPTPLAGWMSSSSSVPHPAVSGGAIALGAPSIQALKHPRTPPSNSAVDYP SGDSDHVSKRTRPMGISDEVSLGVNMLPMTFPGQAHGHTQAFKAPDDLPKTVARTLSQG SSPMSMDFHPIKQTLLLVGTNVGDIGLWEVGSRERLVQKTFKVWDLSKCSMPLQAALVK EPWSVNRVIWSPDGSLFGVAYSRHIVQLYSYHGGEDMRQHLEIDAHVGGVNDIAFSTP NKQLCFTTCGDDKTIKVWDAATGVKRHTFEGHEAPVYSICPHYKENIQFIFSTALDGKI KAWLYDNMGSRVDYEAPGRWCTTMAYSADGTRLFSCGTSKDGDSYIVEWNESEGAVKRT S AA YQGFHKRSLGWQFDTTKNRYLAAGDDFSIKFWDMDNVQLLTAIDADGGLQASPRIRFN 59 KEGSLLAVSANDNMIKVMANTDGLRLLHTVDNLSSESSKPAINNIAVAERAAERPASW SIPGMNGDSRNMVDVKPVITEESNDKSKIWKLTEVGEPSQCRSLRLPENMRVTKISRLI FTNSGNAILALASNAIHLLWKWQRNDRNATGKATASLPPQQWQPASGILMTNDVTETNS EEAVPCFALSKNDSYVMSASGGKISLFNMMTFKTMATFMPPPPAATFLAFHPQDNNIIA IGMDDSTIQIYNVRVDEVKSKLKGHSKRITGLAFSNVLNVLVSSGADAQICVWNTDGWE KQRSKVLQVPQGRSTGALSDTRVQFHQDQVHFLWHETQLAIYETTKLECMKQWPVRES SAPITHATFSCDSQLIYASFMDATICVFTSANLRLRCRVNPSAYLPASLSNSNVHPLVI AAHPQESNMFAVGLSDGGVHIFEPLESEGKWGVAPPPENGSSSALASAPSVGASASDQP QR Sar S 3202.G8615.T1: IAA16 transcription factor ATGTCTTCTCTCAGTAGAGAGCTCGTGTTCTTGATCTTACAGTTTCTAGATGAAGAGAA GTTCAAAGAGACTGTTCATAAGCTGGAACAAGAGTCTGGGTTTTTCTTCAATATGAAGT ATTTTGAGGATGAAGTGCACAGTGGGAACTGGGATGAGGTGGAGAAGTATCTCTCTGGT TTTACCAAAGTTGATGATAATAGATACTCCATGAAGATATTCTTCGAGATTAGGAAGCA GAAGTATCTCGAGGCCTTGGATAAGCATGATCGTCCAAAGGCTGTTGAGATTTTAGTGA AAGATTTGAGAGTGTTTTCCACTTTTAATGAGGAGCTTTTCAAGGAAATCACTCAGCTC TTGACCTTAGAGAACTTTCGGGAGAACGAGCAGTTATCCAAGTACGGAGACACAAAGTC AGCCAGATCTATCATGTTGGTGGAACTTAAGAAGTTGATTGAAGCGAATCCATTATTCC GTGATAAGCTGCAGTTTCCTACCCTTAGAAACTCACGGCTGAGAACGCTGATTAACCAG AGCTTAAACTGGCAACATCAGCTTTGTAAAAACCCAAGGCCAAATCCTGATATCAAGAC NT TCTCTTTGTGGATCACTCCTGTCGCTTACCAAATGATGCACGAGCACCATCCCCTGTCA 60 ACAATCCGCTGCTTGGATCACTACCAAAAGCCGGGGGATTTCCTCCTTTAGGTGCACAT GGGCCATTTCAACCAACACCTTCTCAGGTTCCGACACCTCTTGCTGGTTGGATGTCTAG TTCTTCCTCTGTTCCTCATCCAGCTGTGTCTGGAGGAGCCATAGCTCTAGGTGCTCCAT CCATCCAAGCCTTGAAGCACCCGAGAACTCCTCCAAGTAATTCCGCTGTAGACTATCCA TCAGGTGATTCGGACCATGTCTCAAAAAGAACAAGACCTATGGGAATCTCTGACGAGGT GAGTCTTGGCGTGAACATGTTACCGATGACATTCCCGGGACAGGCACATGGTCATACCC AAGCTTTCAAAGCACCTGATGACTTGCCTAAGACAGTAGCACGAACTTTGAGCCAAGGC TCTTCTCCCATGAGCATGGATTTCCATCCAATCAAACAGACTCTCCTACTCGTTGGTAC AAATGTTGGGGATATTGGGCTCTGGGAAGTCGGTTCTCGAGAACGTCTAGTACAAAAGA CTTTCAAAGTTTGGGACTTGAGTAAATGTTCAATGCCCTTGCAGGCTGCTTTAGTGAAA GAACCTGTTGTTTCTGTCAACCGTGTGATTTGGAGCCCAGATGGTTCTTTATTCGGAGT TGCTTATTCGAGACATATTGTACAGCTATACTCTTATCACGGTGGTGAAGATATGAGAC AACACCTTGAGATTGATGCTCATGTTGGTGGTGTAAATGACATTGCATTCTCCACTCCA AACAAGCAACTATGTTTTACTACTTGTGGTGATGACAAAACCATCAAGGTCTGGGATGC TGCAACGGGTGTAAAAAGGCATACTTTCGAAGGCCATGAAGCTCCTGTTTACTCTATCT GCCCTCATTACAAGGAAAACATTCAGTTCATCTTTTCAACTGCGCTTGACGGGAAAATA AAAGCGTGGCTATATGATAATATGGGTTCTCGGGTTGACTACGAAGCTCCTGGTCGCTG GTGTACAACGATGGCCTACAGTGCTGATGGAACTCGGCTATTTTCTTGTGGGACGAGTA AAGATGGGGATTCATACATAGTTGAGTGGAATGAAAGCGAAGGAGCTGTTAAGAGAACT TATCAAGGATTCCACAAGCGTTCTCTTGGTGTCGTTCAGTTTGATACTACTAAAAACCG CTATCTTGCTGCTGGTGATGACTTCTCCATTAAGTTCTGGGATATGGACAATGTACAGC TGTTGACTGCCATTGATGCTGATGGTGGTCTTCAGGCAAGCCCACGGATCCGGTTTAAC AAGGAAGGCTCTCTCTTGGCCGTTTCTGCCAATGACAATATGATTAAGGTTATGGCAAA CACAGATGGTTTAAGGCTACTGCACACGGTCGACAACTTATCTTCTGAATCCTCCAAGC CTGCAATCAACAACATTGCAGTGGCAGAGAGAGCTGCTGAGAGACCTGCCTCTGTTGTT TCCATCCCTGGAATGAATGGAGATTCACGGAATATGGTAGATGTGAAGCCAGTGATCAC CGAAGAAACAAATGA AAG C AAGA A GGAAGCTTACTGAAGTCGGTGAACCCTCTC AATGCCGCTCATTGAGACTCCCTGAGAATATGAGAGTCACCAAGATATCGAGATTGATT TTCACAAATTCTGGAAATGCGATATTGGCATTGGCATCAAACGCTATTCATCTGCTTTG GAAATGGCAGCGAAATGACCGTAACGCTACTGGAAAGGCAACGGCTTCACTACCTCCTC AGCAGTGGCAACCAGCGAGCGGAATCTTAATGACAAACGATGTGACTGAAACTAATTCA GAGGAAGCTGTACCGTGTTTTGCTTTATCCAAGAATGATTCATATGTGATGTCAGCATC TGGAGGAAAGATCTCTTTGTTTAATATGATGACGTTTAAGACAATGGCTACTTTCATGC CGCCTCCGCCTGCAGCAACGTTTCTGGCTTTTCACCCCCAGGACAACAATATAATTGCA ATTGGAATGGATGATAGCACAATACAGATTTACAATGTTCGCGTTGATGAGGTCAAGAG CAAGCTGAAAGGACATTCCAAGAGAATAACCGGCCTCGCCTTCTCCAACGTACTAAATG TCCTGGTTTCGTCTGGAGCAGACGCTCAGCTTTGTGTCTGGAACACGGATGGATGGGAG AAGCAGAGGAGCAAGGTTCTGCAAGTTCCACAGGGAAGATCAACGGGAGCACTTTCAGA CACGCGTGTTCAGTTTCATCAAGATCAAGTACACTTCCTCGTGGTCCATGAAACTCAGC TCGCTATATACGAAACAACCAAGCTTGAATGCATGAAACAGTGGCCTGTGCGTGAATCA TCAGCTCCAATCACACATGCCACGTTCTCATGTGATAGCCAGTTGATATACGCAAGTTT CATGGATGCTACAATCTGTGTATTCACCTCTGCAAACCTTCGATTGCGTTGCAGAGTCA ATCCCTCTGCGTATTTGCCTGCTTCTCTCAGCAACTCGAACGTCCATCCGCTGGTGATT GCGGCTCATCCTCAAGAATCCAATATGTTTGCTGTGGGTCTCTCAGACGGTGGGGTCCA TATATTCGAGCCGCTCGAGTCAGAAGGTAAATGGGGAGTGGCTCCGCCACCTGAGAACG GCTCATCCAGCGCTTTGGCGTCTACACCTTCCGTTGGAGCTTCCGCATCCGATCAGCCT CAGAGATAA MSSLSRELVFLILQFLDEEKFKETVHKLEQESGFFFNMKYFEDEVHSGNWDEVEKYLSG FTKVDDNRYSMKIFFEIRKQKYLEALDKHDRPKAVEILVKDLRVFSTFNEELFKEITQL LTLENFRENEQLSKYGDTKSARSIMLVELKKLIEANPLFRDKLQFPTLRNSRLRTLINQ SLNWQHQLCKNPRPNPDIKTLFVDHSCRLPNDARAPSPVNNPLLGSLPKAGGFPPLGAH GPFQPTPSQVPTPLAGWMSSSSSVPHPAVSGGAIALGAPSIQALKHPRTPPSNSAVDYP SGDSDHVSKRTRPMGISDEVSLGVNMLPMTFPGQAHGHTQAFKAPDDLPKTVARTLSQG SSPMSMDFHPIKQTLLLVGTNVGDIGLWEVGSRERLVQKTFKVWDLSKCSMPLQAALVK EPWSVNRVIWSPDGSLFGVAYSRHIVQLYSYHGGEDMRQHLEIDAHVGGVNDIAFSTP NKQLCFTTCGDDKTIKVWDAATGVKRHTFEGHEAPVYSICPHYKENIQFIFSTALDGKI KAWLYDNMGSRVDYEAPGRWCTTMAYSADGTRLFSCGTSKDGDSYIVEWNESEGAVKRT YQGFHKRSLGWQFDTTKNRYLAAGDDFSIKFWDMDNVQLLTAIDADGGLQASPRIRFN 6 1 KEGSLLAVSANDNMIKVMANTDGLRLLHTVDNLSSESSKPAINNIAVAERAAERPASW SIPGMNGDSRNMVDVKPVITEETNDKSKIWKLTEVGEPSQCRSLRLPENMRVTKISRLI FTNSGNAILALASNAIHLLWKWQRNDRNATGKATASLPPQQWQPASGILMTNDVTETNS EEAVPCFALSKNDSYVMSASGGKISLFNMMTFKTMATFMPPPPAATFLAFHPQDNNIIA IGMDDSTIQIYNVRVDEVKSKLKGHSKRITGLAFSNVLNVLVSSGADAQLCVWNTDGWE KQRSKVLQVPQGRSTGALSDTRVQFHQDQVHFLWHETQLAIYETTKLECMKQWPVRES SAPITHATFSCDSQLIYASFMDATICVFTSANLRLRCRVNPSAYLPASLSNSNVHPLVI AAHPQESNMFAVGLSDGGVHIFEPLESEGKWGVAPPPENGSSSALASTPSVGASASDQP QR Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame Sar_R_21966.G53064.Tl (SEQ ID NO:62), and encodes an IAA12 bodenlos/monopteros transcription factor (SEQ ID NO:63) to provide the auxin herbicide tolerance. See Table 21. In another aspect, the nucleic acid of SEQ ID NO:62 that encodes the polypeptide of SEQ ID NO:63 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises &r_S_12886.G29900.Tl/ S r_S_59713.G71763.Tl (SEQ ID NO:64), and encodes an IAA12 bodenlos/monopteros transcription factor (SEQ ID NO:65). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. AATTGGGTCACACAGGATGAACAAGGTGAACAACCAAGCTATGAAGGCGGTCAAAGTCG ACGACGAAGAACAAGAAGGGAAGAAGAAGAATGATGAGCC AAAGA GTCTTCTCAGTG AATGGGAATGTTCATGGTCTAGGGTATGTGAAAGTGAACATGGATGGAGTTGGTATAGG CAGAAAAGTGGATATGAGAGCTCATACGTCTTATGAAAACTTGGCTCAGACGCTTGACG AGATGTTCTTCGGAATGACACGTGCTACTACTTCACGGGAAAAGGTGAAACCTTGGACA CTTTTAGACGGATCATCAGAGTTTGTACTCACTTATGAAGACAAGGAGGGAGATTGGAT GCTGGTTGGAGATGTCCCATGGAGAATGTTTGTCACTTCAGTGAAAAGGGTTCGGATCA TGGGAACCTCGGAAGCTAATGGACTTGCTCCAAGACATCAAGAACAGAAAGAGAGACAG AGAAAATAA MRCGGSEPETGKKSNLLPAESELELGLGLSIGGGAWKERGRILTAKDFPSVGSKRAADS SSHHQGASPPRSSQIVGWPPIGSHRMNKVNNQAMKAVKVDDEEQEGKKKNDEPKDVFSV 16 S AA NGNVHGLGYVKVNMDGVGIGRKVDMRAHTSYENLAQTLDEMFFGMTRATTSREKVKPWT 65 LLDGSSEFVLTYEDKEGDWMLVGDVPWRMFVTSVKRVRIMGTSEANGLAPRHQEQKERQ RK

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from 5. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame 5ar_R_36336.g63877.tl (SEQ ID NO:66), and encodes RUBl (SEQ ID NO:67) to provide the auxin herbicide tolerance. See Table 22. In another aspect, the nucleic acid of SEQ ID NO:66 that encodes the polypeptide of SEQ ID NO:67 provides the DART auxinic herbicide tolerance trait to a plant. In one embodiment, the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises 5ar_S_7171.gl8066.tl (SEQ ID NO:68), and encodes RUBl (SEQ ID NO:69). See Table 22. In another embodiment, an additional auxin herbicide susceptible nucleic acid open reading frame is 5ar_S_16564.g36270.tl (SEQ ID NO:70), and encodes RUBl (SEQ ID NO:71). In another embodiment an additional auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises 5ar_S_19347.g4081 1.tl (SEQ ID NO:72), and encodes RUBl (SEQ ID NO:73). See Table 22. In another embodiment an additional auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises 5ar_S_23559.g46658.tl (SEQ ID NO:74), and encodes RUBl (SEQ ID NO:75). See Table 22. In another embodiment, any of the auxin herbicide susceptible nucleic acids is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. Table 22. S. arvensis Auxin-Herbicide Tolerance Trait Sequence Genes 17-19 Nucleic Acid or Amino Acid Sequence SEQ ID Sar R 36336.g63877.tl: RUB1 ATGCAGATCTTCGTGAAAACCTTGACCGGAAAAACCATAACCCTCGAGGTTGAAAGCAG CGACACCATCGACAATGTCAAATCCAAAATCCAGGACAAAGAGGGGATACCACCTGATC AACAGAGACTCATCTTCGCCGGGAAACAACTCGAAGATGGTCGAACGCTAGCCGACTAC AACATCCAGAAAGAGTCCACTCTTCACTTGGTTCTGAGGCTTAGGGGTGGGACCATGAT 17 RNT CAAGGTCAAGACTCTCACTGGTAAAGAGATCGAGATCGATATCGAACCAACCGACACTA 66 TTGATCGGATCAAGGAACGTGTTGAGGAGAAAGAAGGCATCCCTCCTGTTCAACAAAGG CTCATCTATGCGGGGAAACAGCTGGCTGATGACAAAACGGCAAAGGACTACAACATAGA GGGAGGCTCTGTTCTTCATCTGGTCCTTGCTCTTAGGGGTGGTTCTGACTAG MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 17 RAA NIQKESTLHLVLRLRGGTMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQR 67 L YAGKQLADDKTAKDYN EGGSVLHLVLALRGGS D Sar S 7171.gl8066.tl: RUB1 ATGCAGATCTTCGTCAAAACCCTGACCGGGAAAACCATAACCCTAGAGGTTGAAAGCAG CGACACCATCGACAATGTCAAAGCCAAAATCCAGGACAAGGAGGGAATACCACCGGATC AGCAGAGGCTGATATTCGCTGGTAAACAACTTGAAGATGGTCGAACGCTTGCGGACTAC AACATCCAGAAAGAGTCTACTCTTCACTTGGTTCTGAGGCTTAGGGGTGGGACCATGAT 17 S N T CAAGGTCAAGACTCTCACTGGCAAAGAAATCGAGATTGATATCGAACCTACCGACACCA 68 TTGATCGTATCAAGGAACGTGTTGAGGAGAAAGAAGGCATCCCTCCTGTTCAACAAAGG CTCATCTACGCTGGGAAACAGCTAGCTGATGACAAAACGGCAAAAGACTACAACATCGA GGGAGGCTCTGTTCTGCATCTAGTTCTTGCTCTTAGGGGTGGTTTTGGTCTTCTCTAG MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 17 S AA NIQKESTLHLVLRLRGGTMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQR 69 L YAGKQLADDKTAKDYN EGGSVLHLVLALRGGFGLL Sar S 16564.g36270.tl: RUB1 ATGCAGATATTCGTGAAAACCTTGACCGGAAAAACCATAACCCTAGAAGTTGAAAGCAG CGACACCATCGACAATGTCAAATCCAAAATCCAGGACAAAGAAGGGATACCACCTGATC AGCAGAGGCTCATCTTTGCTGGGAAACAACTAGAGGATGGTCGAACGCTGACTGACTAC AACATCCAGAAAGAGTCCACTCTTCACTTGGTCTTGAGGCTTAGGGGTGGCACCATGAT 18 S N T CAAGGTCAAGACTCTCACTGGCAAAGAAATCGAGATTGATATCGAACCTACCGACACTA 70 TTGATCGCATCAAGGAGCGTGTTGAGGAGAAAGAAGGCATCCCTCCTGTCCAACAAAGG CTCATATACGCCGGAAAACAGCTGGCTGATGACAAAACGGCAAAGGACTACAACATCGA GGGAGGCTCTGTTCTTCATCTGGTCCTTGCTCTTAGAGGTGGTGGTTTTGCTCTTTTCT GA MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLTDY 18 S AA NIQKESTLHLVLRLRGGTMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQR 7 1 L IYAGKQLADDKTAKDYN IEGGSVLHLVLALRGGGFALF Sar S 19347.g40811.tl: RUB1 ATGCAGATCTTCGTGAAAACCCTCACCGGAAAAACGATAACCCTAGAGGTGGAGAGCAG CGACACCATCGACAATGTCAAAGCCAAAATCCAGGACAAGGAAGGTATACCGCCAGACC AGCAAAGGCTCATCTTCGCCGGTAAGCAGCTCGAAGACGGCAGAACCCTAGCCGATTAC AACATCCAGAAAGAGTCTACTCTTCATCTCGTCTTGAGGCTCCGTGGTGGTACCATGAT 19.1 S N T CAAGGTCAAGACTCTCACCGGCAAAGAGATCGAGATTGATATCGAACCAACCGACACTA 72 TCGATCGTATCAAGGAACGTGTCGAGGAGAAAGAAGGCATCCCTCCCGTCCAACAAAGG CTTATCTATGCGGGGAAGCAGCTTGCTGATGACAAGACGGCCAAGGATTATAACATAGA GGGTGGCTCTGTTCTTCATCTGGTTCTTGCTCTTAGGGGTGGTGGTGGTCGTGTTTGA MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADY 19.1 S AA NIQKESTLHLVLRLRGGTMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQR 73 L IYAGKQLADDKTAKDYN IEGGSVLHLVLALRGGGGRV Sar S 23559.g46658.tl: RUB1 ATGCAGATCTTCGTGAAAACCCTGACCGGAAAAACCATAACTCTCGAGGTTGAAAGCAG 19.2 S N T CGACACCATCGACAATGTCAAATCCAAAATCCAGGACAAAGAGGGGATACCACCTGATC 74 AACAGAGACTCATCTTCGCCGGGAAACAACTCGAAGATGGTCGAACGCTTTCTGACTAC AACATCCAGAAAGAGTCCACTCTTCACTTGGTTCTGAGGCTTAGGGGTGGGACCATGAT CAAGGTCAAGACTCTAACCGGTAAAGAGATCGAGATCGATATCGAACCAACCGACACTA TTGATCGGATCAAGGAACGTGTTGAGGAGAAAGAAGGCATCCCTCCTGTTCAACAAAGG CTCATATATGCGGGGAAACAGCTGGCTGATGACAAAACGGCAAAGGACTACAACATTGA GGGAGGCTCTGTTCTTCATCTGGTCCTTGCTCTTAGGGGTGGTTCTGACTAG MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDY 19.2 S AA NIQKESTLHLVLRLRGGTMIKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQR 75 L YAGKQLADDKTAKDYN EGGSVLHLVLALRGGS D

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame 5ar_R_16466.g45572.tl (SEQ ID NO:76), and encodes a CAM7 transcription factor (SEQ ID NO:77) to provide the auxin herbicide tolerance. See Table 23. In another aspect, the nucleic acid of SEQ ID NO:76 that encodes the polypeptide of SEQ ID NO:77 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises 5ar_S_29545.g53829.tl (SEQ ID NO:78), and encodes a CAM7transcription factor (SEQ ID NO:79). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide.

Another embodiment described herein is a nucleic acid that provides auxin herbicide tolerance to a plant. In one aspect, the auxinic herbicide tolerance trait is DART. In some aspects, the nucleic acid is an isolated, mutagenized, or recombinant nucleic acid from S. arvensis. In another aspect, the auxin herbicide tolerance nucleic acid comprises the open reading frame Sar_R_3970.gl4672.tl (SEQ ID NO:80), and encodes a cytochrome P450 CYP83A1 (SEQ ID NO:81) to provide the auxin herbicide tolerance. See Table 24. In another aspect, the nucleic acid of SEQ ID NO:80 that encodes the polypeptide of SEQ ID NO:81 provides the DART auxinic herbicide tolerance trait to a plant. In some embodiments the auxin herbicide susceptible nucleic acid corresponding to the auxin herbicide tolerance nucleic acid comprises Sar_S_51371.g68743.tl (SEQ ID NO:82), and encodes a cytochrome P450 CYP83A1 (SEQ ID NO:83). In another aspect, the auxin herbicide susceptible nucleic acid is mutagenized to create an auxin herbicide tolerant polynucleotide encoding an auxin herbicide tolerant polypeptide. AGTDTAAAAWWGMT YLMKYPQVMKKSQAEVRE YARE KGST EDDLNNLPYFK ALVKETLRIEPVI PLLVPRAC IQDTKIAGYDI PAGTT INVNAWAVSRDEKEWGPNPDEF RPERFLEKDVDFKGTDYEFI PFGSGRRMCPGMRLGTAMLEVPYANLLLNFDFKLPDGMK PEE INMDVMTGLAMHKSDHLKLVPQKVSK Sar S 51371.g68743.tl: cytochrome P450 CYP83A1 ATGGAAGATGTCATCATCGGCGTGGTGGCTATCGCTGCAGTTCTCCTCTTGTTCCACTA CCAAAGAAAAAAAACCAAACGGTACAAGCTACCTCCGGGACCAAAGGCACTTCCGGTCA TCGGAAACCTCCACCAGCTTCAGAGCCTTAACCCACAACGGTTCTTCTGTGGATGGGCC AAAAAATACGGACCAATCTTGTCATACAAGATAGGAAGTAGAACAATGGTGGTGATATC TTCGGCTGAACTAACCAGAGAGCTTCTCAAGACGCAAGACGTCAACTTTGCAGACCGGC CTGTGCACCGTGGCCAGGAGTTCATGTCCTACGGCCGACGTAACATGGCGTTTCATCAT TATACACCGTATTACCGGGATTTAAGGAAGATGGCGATGAACCACTTGTTCTCACCCAC ACGTGTGGCCACCTTTAAGCACGTACGGGAGGAGGAGGCTAAGAGGATGATGGATAAGA 2 1 S NT 82 TCAGTGACGCCGCTGATAAATCCACAGCGGTCGATATAAGCGAGCTTATGTTGACATTC ACCAACTCCGTTGTGTGTAGACAAGCGTTCGGGAAGAAGTACAATGAAGATGGGGAAGA GATGAAAAGGTTCATCAAGATTCTTTATGGGAGTCAGAGCGTTTTGGGGAAGAATTTTT TCTCTGATTCTTTTCCTTTTACTGGCTACGTTCTCGACGATTTGACGAAGCTCACCGCT ATATGAAAGAATGTTTCGAAAGACAAGACACTTATTTGCAAGAGATTATCGATGAAAC GCTTGATCCCAATAGGGCCAAGCCTGAAACCGAGAGCATGATTGATCTCTTGATGGAGA TCTACAGAGATCAACCTTTCGCCTCCAAGTTCACTCTTGAGAATGTCAAAGCCGTGGTT CTGTGA MEDVI IGWAIAAVLLLFHYQRKKTKRYKLPPGPKALPVIGNLHQLQSLNPQRFFCGWA KKYGPI LSYKIGSRTMWI S SAELTRELLKTQDVNFADRPVHRGQEFMSYGRRNMAFHH YTPYYRDLRKMAMNHLFS PTRVATFKHVREEEAKRMMDKI SDAADKSTAVDI SELMLTF 2 1 S AA TNSWCRQAFGKKYNEDGEEMKRFIKI LYGSQSVLGKNFFSDSFPFTGYVLDDLTKLTA 83 YMKECFERQDTYLQE DETLDPNRAKPETESMI DLLME IYRDQPFASKFTLENVKAW L

In another embodiment described herein, the nucleic acids comprise polynucleotides having nucleotide sequences that encode polypeptides comprising the amino acid sequences of any one ofSEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 81. In other embodiments, the nucleic acid molecules comprise polynucleotides comprising the nucleotide sequences of any one of SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, or degenerate, homologous, or codon-optimized variants thereof. A polynucleotide having a nucleotide sequence at least, for example, 90% "identical" to a reference nucleotide sequence encoding a polypeptide, as used herein, is intended to mean that the nucleotide sequence of the polynucleotide be identical to the reference sequence except that the polynucleotide sequence can include up to about ten substitutions or point mutations per each 100 nucleotides of the reference nucleotide sequence. In other words, to obtain a polynucleotide having a nucleotide sequence about 0% identical to a reference nucleotide sequence, up to 10% of the nucleotides in the reference sequence can be deleted or substituted with another nucleotide, or a number of nucleotides up to 10%> of the total nucleotides in the reference sequence can be inserted into the reference sequence. These substitutions or mutations of the reference sequence can occur at the 5'- or 3'-terminal positions of the reference nucleotide sequence or anywhere between those terminal positions, interspersed either individually among nucleotides in the reference sequence, or in one or more contiguous groups within the reference sequence. As noted above, two or more polynucleotide sequences can be compared by determining their percent identity (or "homology"). Two or more amino acid sequences likewise can be compared by determining their percent identity. The percent identity of two sequences, whether nucleic acid or peptide sequences, is generally described as the number of exact matches between two aligned sequences divided by the length of the shorter sequence and multiplied by 100. Numerous applications are available for preforming sequence comparisons and alignments, e.g., BLAST. Altschul et al, (1990); Altschul et al, (1997). For example, due to the degeneracy of the genetic code, one having ordinary skill in the art will recognize that a large number of the nucleic acid molecules having a sequence at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to the nucleic acid sequence shown in SEQ ID NOs:8, 12, 16, 18, 22, 26, 30, 34, 36, 40, 44, 48, 52, 56, 62, 66, 76, or 80, or degenerate, homologous, or codon-optimized variants thereof. The polynucleotides described herein include those encoding mutations, variations, substitutions, and particular examples of the polypeptides described herein. For example, guidance concerning how to make phenotypically silent amino acid substitutions is known in the art. Thus, fragments, derivatives, or analogs of the polypeptides of SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 8 1 can be (i) ones in which one or more of the amino acid residues (e.g., 1, 2, 3, 4, 5, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 25, 30, 35, 40, 45, or 50 residues, or even more) are substituted with a conserved or non-conserved amino acid residue (preferably a conserved amino acid residue). Such substituted amino acid residues may or may not be one encoded by the genetic code, or (ii) ones in which one or more of the amino acid residues includes a substituent group (e.g., 1, 2, 3, 4, 5, 7, 8, 9, 10, 15, 20, 25, 30, 35, 40, 45, or 50 residues or even more), or (iii) ones in which the mature polypeptide is fused with another compound, such as a compound to increase the half-life of the polypeptide (for example, polyethylene glycol), or (iv) ones in which the additional amino acids are fused to a mature polypeptide, such as an IgG Fc fusion region peptide or leader or secretory sequence or a sequence which is employed for purification of the mature polypeptide or a proprotein sequence. Such fragments, derivatives, and analogs are deemed to be within the scope of those skilled in the art from the teachings herein. In addition, fragments, derivatives, or analogs of the polypeptides of SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 8 1 can be substituted with one or more conserved or non-conserved amino acid residues. In some aspects, an amino acid residue will be substiuted with a conserved amino acid residue. In some cases these polypeptides, fragments, derivatives, or analogs thereof will have a polypeptide sequence at least 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to the polypeptide sequence shown in SEQ ID NOs:9, 13, 17, 19, 23, 27, 31, 35, 37, 41, 45, 49, 53, 57, 63, 67, 77, or 8 1 and will comprise functional proteins or enzymes. Preferably, the native function of the polypeptide will be retained by any such substitutions. As described herein, in many cases the amino acid substitutions or mutations are preferably of a minor nature, such as conservative amino acid substitutions that do not significantly affect the folding or activity of the protein. Of course, the number of amino acid substitutions a skilled artisan would make depends on many factors, including those described herein. Generally, the number of substitutions for any given polypeptide will not be more than about 100, 90, 80, 70, 60, 50, 40, 30, 25, 20, 19, 18, 17, 16, 15, 14, 13, 12, 11, 10, 9, 8, 7, 5, 6, 4,

3, 2, or 1. The polypeptides described herein can be provided in an isolated form. The phrase "isolated polypeptide" implies that a polypeptide is removed from its native environment. Thus, a polypeptide produced and/or contained within a recombinant cultured host cell is considered isolated for purposes described herein. Further, "isolated polypeptides" are polypeptides that have been purified, partially or substantially, from a recombinant cultured host. Polypeptides having an amino acid sequence of an indicated percent identity to a reference amino acid sequence can be determined using methods, including computer-assisted methods. Polypeptide amino acid sequences are examined and compared just as are the nucleotide sequences in the foregoing discussion. One of skill in the art will recognize that such concepts as the molecular endpoints discussed for polynucleotides will have direct analogs when considering the corresponding use of such methods and programs for polypeptide analysis. Another embodiment described herein is a method for identifying an auxinic herbicide tolerant plant, or plant part thereof, the method comprising: (a) providing biological material from a plant comprising the DART trait; (b) performing PCR, hybridization testing, or sequencing of said nucleic acid in said biological material to determine if said plant comprises the DART trait; and (c) identifying, based on the results of step (b), that the plant comprises the DART trait. In one aspect, the plant comprises: (i) any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture

Collection (ATCC) under Patent Deposit Designation Numbers PTA-120132, PTA-1 121 1, PTA- 12050, PTA-1 1212, PTA-1 1213, and PTA-1 1214, respectively; (ii) a mutant, recombinant, or a genetically engineered derivative of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R and that expressed the DART trait thereof; or (iii) a plant that is a progeny of at least any one of the plants of (i)-(ii) and that expressed the DART trait thereof. In another aspect, the biological material comprises a Brassica or a Raphanobrassica plant, plant part thereof, seed, or cell.

It will be readily apparent to one of ordinary skill in the relevant arts that suitable modifications and adaptations to the compositions, methods, and applications described herein can be made without departing from the scope of any embodiments or aspects thereof. Having now described the inventions in detail, the same will be more clearly understood by reference to the following examples, which are included herewith for purposes of illustration only and are not intended to be limiting. The scope of the compositions and methods described herein include all actual or potential combinations of aspects, embodiments, examples, and preferences herein described. All patents and publications cited herein are entirely incorporated by reference herein for the specific teachings thereof. EXAMPLES

EXAMPLE 1: Dicamba Tolerant Canola Two dicamba tolerant Sinapis arvensis lines (DT-01 SA2-R and DT-01 BC8SA2-R), 3 Brassica napus lines (Westar, Topaz, and BN02), and 1Brassicajuncea line (Arid) were used as pollen recipients and donors in the initial crosses. Later pollinations used the S. arvensis lines only as the male. On the third day after pollination, entire inflorescence stalks (peduncles) with pollinated buds were collected from the plants and the cut ends of the flower stalks were placed in a container of water. All petals and sepals were removed from the flowers and the remaining ovary, together with the flower pedicel, were placed in a 15 mL or 50 mL sterile tube for surface sterilization. Ovaries were surface sterilized in 70% ethanol for 2-3 minutes followed by 10% commercial (Clorox) bleach for 10 minutes. Ovaries were then rinsed 3 times with sterile distilled water, each ovary was cut at the base to remove the pedicel, and cultured upright on BOCBN medium (Table 25). After 30-45 days in culture, ovaries were cut open and in vitro seed was removed and cultured on BOCBN medium. Germinating seeds were transferred to M-LS-513 medium {see Table 25) in PlantCons and were allowed to grow until plants developed good roots. All in vitro material was grown at 23 °C with 16 hours light. Plants were then transplanted into Metro-mix potting soil in the greenhouse and grown to maturity. Peduncle cultures were initiated from inflorescences of 22, Fl plants in an effort to stimulate chromosomal recombination. The chosen peduncles had closed flower buds and few open flowers. Only the youngest, soft, green tissue was used. Flower buds and pedicels were discarded. The peduncles were surface sterilized with 70% ethanol for 5 minutes, followed by 10% commercial (Clorox) bleach for 10 minutes. The peduncles were rinsed three times with sterile distilled water and the damaged ends were removed. The peduncles were then cut into explants approximately 5 mm long and were cultured horizontally on BPC-BAP medium (Table 25). Callus formed at the cut ends of the peduncle explants usually within 2 weeks, after which shoot buds, and later, shoots regenerated. After 3-4 weeks of culture, the entire regenerating ends of explants (shoot pads) were removed and transferred to BPC-0 medium for shoot elongation. Individual shoots were excised and cultured on M-LS-513 medium for further growth and rooting. Each shoot regenerated from peduncle culture was further propagated in vitro and maintained on M-LS-513 medium. An in vitro dicamba kill curve was developed for screening seedlings and BC1 plants. Seed of dicamba susceptible (B. napus line BN02 and S. arvensis S line) and resistant (S. arvensis saBC8R and saParR) lines were surface sterilized with 70% ethanol for 2 minutes, then with 30% commercial (Clorox) bleach for 10 minutes, followed by three rinses with sterile distilled water. Seeds were placed on M-LS-513 medium in PlantCons and were allowed to germinate at 23 °C and 16 hours light. When seedlings were 14-21 days old, M-LS-504C medium was melted and mixed with various amounts of dicamba solution to final concentrations ranging from 0-200 mg/L. The dicamba-504C medium combination was then poured on top of the existing M-LS-513 medium, allowed to harden, and the PlantCons were placed back in the growth chamber. After 2-3 weeks of exposure to dicamba, the plants were evaluated. A level of 2.5 mg/L dicamba was determined to give the best differential response (Figure 1). The development of an efficient in vitro embryo rescue was useful for producing hybrid plants from Brassica napus Sinapis arvensis crosses. Once the protocol was developed, over 2,000 ovaries and 686 individual ovules were cultured to produce 37 putative Fl plants (Tables 26 and 27). All Fl plants were B. napus crossed with dicamba tolerant (DT) S. arvensis. An in vitro embryo rescue also was performed for producing hybrid plants from Brassica juncea Sinapis arvensis crosses. Table 27. Summary of Fl hybrid plants produced. Experiment Appearance of Confirmed Plant ID Cross ID Plant Hybrid SNPs BN02xSa-A LABI 11008 BN02 DT-01 BC8SA2-R lots of trichomes Y BN02xSa-B LABI 11008 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-C LAB 120408 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-D LABI 12108 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-E LABI 12008 BN02 x DT-01 SA2-R lots of trichomes Y BN02xSa-F LAB 120408 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-G LAB 120808 BN02 x DT-01 SA2-R lots of trichomes Y BN02xSa-H LAB 120808 BN02 x DT-01 SA2-R lots of trichomes Y BN02xSa-I LAB 120808 BN02 x DT-01 SA2-R lots of trichomes Y BN02xSa-J LAB 120808 BN02 x DT-01 SA2-R lots of trichomes Y BN02xSa-K LAB 120808 BN02 x DT-01 SA2-R lots of trichomes Y BN02xSa-L LABI 11008 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-M LABI 11008 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-N LABI 11408 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-O LAB 120808 BN02 x DT-01 SA2-R lots of trichomes Y BN02xSa-P LAB0 12209 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-Q LAB020209 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-R LAB020509 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-S LAB022309 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-T LAB022609 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-U LAB030509 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-V LAB0 12209 BN02 x DT-01 BC8SA2-R lots of trichomes Y BN02xSa-W LAB020209 BN02 x DT-01 BC8SA2-R lots of trichomes NT BN02xSa-X LAB030209 BN02 x DT-01 BC8SA2-R lots of trichomes NT BN02xSa-Y LAB030509 BN02 x DT-01 BC8SA2-R lots of trichomes NT BN02xSa-Z LAB030909 BN02 x DT-01 BC8SA2-R lots of trichomes NT BN02xSa-l LAB030909 BN02 x DT-01 BC8SA2-R lots of trichomes NT BN02xSa-2 LAB030209 BN02 x DT-01 BC8SA2-R lots of trichomes NT BN02xSa-3 LAB0 12209 BN02 x DT-01 BC8SA2-R lots of trichomes NT BN02xSa-4 LAB030209 BN02 x DT-01 BC8SA2-R lots of trichomes NT

looks like normal WestarxSa-A LAB 103 108 Westar x DT-01 SA2-R NT Westar WestarxSa-B LAB 12 1108 Westar x DT-01 BC8SA2-R lots of trichomes Y WestarxSa-C LAB 12 1108 Westar x DT-01 BC8SA2-R lots of trichomes Y looks like normal WestarxSa-D LAB121 108 Westar x DT-01 BC8SA2-R NT Westar

TopazxSa-A LABI 11008 Topaz x DT-01 SA2-R lots of trichomes Y TopazxSa-B LAB 120508 Topaz x DT-01 BC8SA2-R looks normal N TopazxSa-C LAB 121208 Topaz x DT-01 SA2-R looks normal NT S. arvensis DT lines produced many trichomes on the young leaves and stems, while plants from the Brassica lines had very few trichomes. Thirty-three of the 37 putative Fl plants produced from embryo rescue also displayed the S. arvensis trichome trait (Figure 2). Leaf tissue was collected from 26 of the Fl plants as well as the parent lines and sent to DNA Landmark for SNP analysis (Figure 3). The SNP analysis confirmed that the presence of trichomes was correlated with true Fl hybrids. None of the Fl hybrid plants set seed regardless of whether the plant was self-pollinated or crossed with B. napus. Therefore, a second round of embryo rescue was initiated from Fl plants following the same procedure as described below. Over 1,300 ovaries were cultured and 5 BC1 plants were produced (Table 28).

Peduncle culture was also initiated from 22 Fl hybrid plants in an effort to stimulate chromosomal recombination. Christey and Earle, (1991). Plants were regenerated from 12 Fl hybrid lines. All BC1 and peduncle-culture-derived plants were propagated in vitro. Plants were tested for dicamba tolerance using an in vitro dicamba screen as well as spray testing in the greenhouse to confirm introgression of the dicamba tolerant trait. EXAMPLE 2 : Development of Near-Isogenic Lines and Identification of Markers Linked to Auxinic Herbicide Resistance in Wild Mustard {Sinapis arvensis L.).

Raising Plants, Crosses, and Pollinations Unless indicated otherwise, all plants were grown in a growth room in plastic pots containing Promix (Plant Products, Bramalea, Ontario), with a 16 h photoperiod and 22/15 °C day/night temperatures. Light intensity and the relative humidity were maintained at 350 µ οΐ - 1 rr and 65-75%, respectively. Plants were irrigated when required and fertilized weekly with 20:20:20 (N:P:K). Development of homozygous auxinic herbicide-resistant (R; genotype RR) and -susceptible (S; genotype rr) parental lines, genetic crosses to generate F2, and backcross (BC) progeny, pollination methodology and picloram selection have been described. See Jugulam et al. (2005).

Development of Near-Isogenic Lines The protocol used to generate NILs is presented in Figure 4. A total of 8 backcrosses were performed, i.e., B CN F I , where N equals the number of backcrosses. The homozygous recessive S (rr) was used as the recurrent as well as the seed parent, and B C F1 progeny were evaluated for response to picloram (100 g ai/ha). Jugulam et al. (2005). Survivors (genotype Rr) were used as the pollen parent for the next BC generation. Individual BCsFi survivors of the picloram treatment were designated BCsFi/A, where A indicates a randomly assigned number for each BCgFi survivor. Plant BCgFi/l was self-pollinated to generate BCgF2 seed. Each BC F2 plant was raised without picloram selection and self-pollinated to generate BCgF3 seed families

(-40-60 seeds/family), designated BCgF3/B, where B indicates a randomly assigned number for each BCgF2 plant. Twenty individual BCgF3 families were raised and screened with picloram (20 to 30 seedlings per family), to determine the segregation ratio of the R and r alleles and the genotype of the BC8F2/B plants. Homozygous RR and rr BC8F3/B families were chosen as NILs.

Biomass, Seed Yield, Leaf Area and Leaf Shape of NILs At least four plants each of homozygous R and S NILs were treated with picloram (100 g ai/ha, as above) at the four-leaf stage, and as many of each line were untreated to determine the dry weights of roots and shoots 6 weeks after planting. Dry weights were obtained by rinsing with water and drying roots and shoots at 68 °C for 48 h before weighing. Silique number and seed yield were also determined from four other untreated plants of homozygous R and S NILs allowing them to open pollinate naturally. A morphological trait (serrated leaf margin of first true leaf) associated with auxinic herbicide-resistance in wild mustard was observed. This morphological trait was tested for linkage to the auxinic herbicide-resistance gene. Parental R and S, NIL R and S (30 in each), as well as 75 BC4F1 progeny were assessed for this characteristic 1 DAP (days after planting), and first true leaf areas measured 3 1 DAP. All seedlings were scored as serrated or smooth before treatment with picloram to determine the genotype at the auxinic herbicide resistance locus of the

BC4F1plants. Frequencies of R and S phenotypes were tabulated for Fi and backcross populations. Chi-square tests were performed to determine the goodness of fit to specific genetic ratios. The analysis of other data parameters was performed using ANOVA. For the experiments that determined the fitness of wild mustard NILs, data were collected from at least four replications and the experiments were repeated twice.

The progeny from all backcrosses (BC 1F1 to BCgFi) showed similar responses following picloram application (100 g ai/ha; Table 29). Seedlings exhibiting picloram injury one week after treatment exhibited epinasty and eventually died. Remaining backcross progeny showed no picloram injury, and Chi-square tests confirmed 1:1 segregations (R [Rr]:S [rr]) among all B C F I progeny, as expected. Jugulam et al. (2005). NILs were selected from B C 8F 3/B families. Upon picloram application (100 g ai/ha), all plants in the families # 1 and # 8 were unaffected by herbicide treatment, confirming that the genotype of these individuals is 'RR ' (Table 30).

Table 30. Segregation of resistant (R) and susceptible (S) plants among BC8F3 families (raised 1 from self-pollinated BC F2) following treatment with 100 g ai/ha picloram .

1 Resistance and susceptibility were assessed by comparing the response of progeny from backcrosses to the responses of seedlings from R and S parental populations following treatment with picloram (lOO g ai ha 1). 2 Progeny raised from self-pollination of BCgF2. Chi-square values are the results of tests for goodness of fit to a 3 :1 (R:S) segregation model. 4 Probability of accepting or rejecting the null hypothesis. Accept the null hypothesis that progeny segregate 3 :1 (R:S) Reject the null hypothesis that the progeny segregate 3:1 (R:S) 7 Not subjected to χ2because of zero expected value for one class Conversely, in other families, i.e., # 5, # 16, and # 18, all plants exhibited epinasty and eventually died (Table 30), indicating that these families are of the V ' genotype. These families constitute the chosen NILs with auxinic herbicide-R (#1 and 8) and -S (#5, 16, and 18).

Morphological Marker Linked to Auxinic Herbicide Resistance The development of near-isogenic lines (NILs) of a single weed species segregating for auxinic herbicide-R and -S gene variants has not yet been reported. In this study, NILs with auxinic herbicide-R and -S gene variants were developed in wild mustard using repeated- backcrosses. These lines were used in growth room studies to examine plant biomass and seed yield parameters that contribute to plant fitness. A morphological marker (leaf shape) was identified that was closely linked to the auxinic herbicide resistance gene in this weed species. R and S wild mustard biotypes showed differences with regard to the shape of first true leaves (Figure 5). The R biotype plants exhibited a serrated leaf margin whereas the S parental plants had a smooth margin. The leaf area of first true leaves of the plants was also significantly greater for S than R (Figure 6). First true leaves of BC4F 1 (75 plants) were measured for leaf area before picloram treatment, then scored for picloram resistance (Table 31).

χ2 is set for the probability of leaf area unlinked to the resistance locus; the results (χ2 37.9, degrees of freedom: 3; p < 0.001) suggests that the gene loci that control leaf morphology and auxinic herbicide resistance are linked. Linkage Percentage: 14.6% based on the following calculation: # of recombinants/total # of BC4F 1 progeny x 100; [ 1 1/75= 0.146 100 = (14.6 %)]. The serrated leaf margin showed perfect correlation (100%) to the smaller leaf area. The segregation of leaf shape and auxinic herbicide resistance trait confirmed that the smaller leaf area/serrated leaf trait is linked and in phase with the R allele, and demonstrated that the gene controlling leaf shape and the gene encoding the DART auxinic herbicide resistance trait are separated by a distance of 14.6 centimorgan (cM) in the wild mustard genome (Table 31). Analysis of Fitness of NILs Analysis of growth and seed yield of R and S NILs indicated that untreated S plants produced more whole plant biomass and had a greater seed yield than R plants (Table 32).

Conversely, the total plant biomass and seed yield of R plants that survived picloram treatment did not significantly differ from untreated R plants (Table 31).

EXAMPLE 3 : Field Trials of Introgressed Progeny. The field data presented shows auxinic herbicide-tolerance of two plant lines: a Sinapis arvensis line (DT-01 SA2-R) and a Brassica napus line (DT-01 BC4Bn#13-l). The auxinic herbicide-tolerance trait was transferred from the Sinapis arvensis (DT-01 SA2-R) line into the Brassica napus (DT-01 BC4Bn#13-l) line by interspecific crossing. Following introgression, the DT-01 BC4Bn#13-l line was selfed two generations and then subjected to a caged seed increase. The DT-01 SA2-R line and the check lines were also increased in cages in the field. Seed from these caged field productions were used to conduct an herbicide tolerance trial in North Dakota, USA. The three-repetition trial used a randomized split block design. The trial was designed to measure the percent phytotoxicity (% crop injury) observed on the Sinapis arvensis DT-01 SA2- R line versus its non-herbicide-tolerant comparator line, Sinapis arvensis, and the Brassica napus DT-01 BC4Bn#13-l line versus its non-herbicide-tolerant comparator line, Westar. The herbicide used for this study was Clarity (dicamba) with 0.25% NIS (non-ionic surfactant). Three treatment rates of dicamba were applied at the 3-4 leaf stage: 0 g ai/ha dicamba; 240 g ai/ha dicamba; and 480 g ai/ha dicamba. Each plot in the trial was assessed for the level of phytotoxicity (percent crop injury) on a scale from 0-100% at 36 days after treatment. Mean values for each entry across the 3 replications along with their statistical significance (ANOVA) are presented in Table 33.

The percent phytotoxicity (% crop injury) ratings were transposed into Herbicide Tolerance ratings by subtracting the % crop injury rating (Table 33) for each line from 100% to obtain the percent herbicide tolerance, and then calculating the percent herbicide tolerance present in the target lines, DT-01 BC4Bn#13-l and DT-01 SA2-R, as a function of their non- herbicide tolerant checks (Table 34). As used herein in regard to auxinic herbicide-treated plants, terms such as "without significantly inhibiting the growth" of a plant, refer to plants that exhibit about 50% or less phytotoxicity, as measured 36 days after treatment with an auxinic herbicide, as compared to that exhibited by plants of the corresponding wild-type variety, i.e. the same variety lacking the auxinic herbicide tolerant DART trait found in the herbicide tolerant plant. For example, as shown in Table 33, when treated with dicamba at rates of 240 and 480 g ai/ha, the herbicide tolerant B. napus plants exhibit about 56% or less of the phytotoxicity of the corresponding Westar Check line. The mean percent herbicide tolerance was also calculated to show that certain genotypes of Brassica napus have a natural tolerance level to dicamba herbicide. The level of tolerance contributed by the auxinic herbicide-tolerance trait in both DT-01 SA2-R and the Brassica napus DT-01 BC4Bn#13-l lines was significantly higher than the background tolerances observed in their non-herbicide tolerant counterparts (Checks in Table 34).

In a separate study, dicamba was applied to plants of the Brassica napus DT-01 BC4Bn#13-l line as well as to the non-herbicide-tolerant comparator line, each at the 2-3 leaf stage, at a rate equivalent to 240 g ai/ha dicamba in order to further determine plant injury. By 36 days after treatment, the positive controls, non-resistant Brassica plants of the comparator line showed significant visible injury, defined as (a) leaf epinasty and stem epinasty or (b) leaf epinasty and stem swelling, each in conjunction with a delay in maturity/maturation of more than 2 days. By contrast, plants of line Brassica napus DT-01 BC4Bn#13-l showed no significant visible injury at 36 days. These results demonstrate that the auxinic herbicide tolerance trait discovered in Sinapis arvensis and transferred to Brassica napus through interspecific crossing confers a significantly higher level of herbicide tolerance to auxinic herbicides, such as dicamba, than the background tolerance levels observed in the near-isogenic check lines. Additional dicamba-tolerant Brassica germplasm was generated by crossing the dicamba tolerant Sinapis arvensis with Brassica rapa; and dicamba tolerant Sinapis arvensis with Brassica juncea; and Fl hybrids tolerant to 200 g ai/ha dicamba were successfully generated from these crosses. Moreover, the dicamba tolerant Sinapis arvensis Brassica juncea Fl hybrids were pollen fertile and carried through subsequent backcrosses to generate BC3F1 progeny. EXAMPLE 4 : Identification of Markers Linked to Auxinic Herbicide Resistance in Wild Mustard {Sinapis arvensis L.) using Near Isogenic-Lines (NILs). NILs with auxinic herbicide-R and -S were identified upon screening (picloram 100 g ai/ha) BC8F3 families. To identify markers linked to the auxinic herbicide resistance locus, marker analysis was performed using genomic DNA isolated from wild mustard R and S plants chosen from BC1F1, BC7F1, and BC8F1 as well as parental and NILs. Linkage of markers to auxinic herbicide resistance locus was determined by analysis of segregation data from BC progeny. Six markers (Markers 1-6 (M1-M6)), were found linked to R locus and all six markers were sequenced (Figure 7). Based on the recombination frequency, a genetic map was constructed (Figure 8); the genetic distances between the resistance locus and the closely-linked markers ranged from -1.5 to 26 cM.

EXAMPLE 5 : Tolerance of Dicamba-tolerant Sinapis arvensis to Various Auxinic Herbicides. Table 35 shows the tolerance of dicamba-tolerant Sinapis arvensis to auxinic herbicides. EXAMPLE 6 : Transfer of Auxinic Herbicide Tolerance from Sinapis arvensis to Brassica juncea and Brassica rapa through Embryo Rescue. Development of a method for transferring auxinic herbicide tolerance from S. arvensis to B.juncea and B. rapa crops was attempted. This involved significantly altering in vitro culture conditions to obtain a high frequency of embryo regeneration and a high rate of hybrid plant establishment. This work was successful and resulted in a method in which the desired results were achieved, as further described below. Successful transfer of auxinic herbicide tolerance into the hybrids was assessed by whole plant screening of hybrids with dicamba. The hybrids were also tested for fertility (pollen and pistil) and their ability to produce backcross progeny. The auxinic herbicide-resistant trait was further introgressed into B. juncea by backcross breeding. DNA ploidy of the hybrids as well as the backcross progeny was estimated by flow cytometry.

Production of Hybrids between S. arvensis, B.juncea, and B. rapa Auxinic herbicide-R and -susceptible (S) S. arvensis, B.juncea, and B. rapa were raised from seed. The seeds were sown in 6-inch plastic pots containing Promix (Plant Products, Canada), and placed in a growth chamber having a 16 h photoperiod and 22/15 °C day/night temperature. The light intensity and the relative humidity were maintained at 350 µιηοΐ m 2 s 1 and 65-75%, respectively. Each pot contained one plant and the plants were irrigated when required. Plants were fertilized weekly with 20:20:20 (NPK). When plants were flowering, crosses were performed between auxinic herbicide-R S. arvensis and B. juncea or B. rapa following the procedure described by Jugulam et al, (2005). The hybrid seed failed to develop in vivo. Siliques (narrow elongated seed capsules) did not grow completely and ceased before maturity. Therefore, an in vitro method was used for completion of embryo maturation and plantlet formation. To achieve embryo regeneration and plantlet formation via embryo rescue, immature siliques were harvested 3 to 5 d after pollination from crosses between auxinic herbicide-R S. arvensis and B.juncea or B. rapa. Siliques were dismfected with 70% ethanol for 1 to 2 mi l. followed by 20% commercial bleach (sodium hypochlorite, 5.25%) containing three to four drops of Tween* 20 for 5 to 20 min., and subsequently rinsed four to five times with sterile deionized water. The siliques were aseptically cultured in a Petri dish (Fig. 9A) containing 15 mL of either of the following two media: (A). Murashige and Skoog (1962) salts with Gamborg vitamins (1968), sucrose (3%), and 500 mg casein hydrolysate; or (B). Murashige and Skoog (1962) salts with Gamborg vitamins (1968), sucrose (3%), 0.5 mg NAA (naphthalene acetic acid), and 2.5 mg kinetin. The pH of the media was adjusted to 5.8 and 8 g of agar (Difco; micropropagation grade agar was used in all other experiments) was added before autoclaving at 121 °C for 20 min. The siliques were allowed to grow on these media for ~2 weeks. For ovule maturation and regeneration into plantlets, ovules were excised aseptically from the siliques with a forceps and scalpel and cultured on a Petri dish (2 to 3 ovules per dish; Fig. 9B) containing fresh medium (A or B). All cultures were incubated in a growth room at 24 °C in light (16 h photoperiod; 50 µιηοΙ s_ 1) provided by cool white fluorescent lamps (Philips Canada, Scarborough, ON). Four weeks after ovule culture, hybrid plant regeneration occurred (Fig. 9C) and these young plantlets were subsequently transferred individually to a Magenta box (300 mL plastic vessels, Magenta Corp., Chicago IL, USA) containing medium C). Murashige and Skoog (1962) salts with Gamborg vitamins (1968) and sucrose (1.5%). The pH of medium C was adjusted to

5.8 and 8 g of agar was added before autoclaving at 121 °C for 20 min. After four weeks on medium C, the hybrids developed roots and shoots. Hybrid plants were clonally multiplied by aseptically culturing nodal segments in Magenta boxes containing medium C. After six weeks of culture, nodal segments with well-developed roots and shoots were transferred to soil (Promix) and grown under the same conditions as described before for growing S. arvensis and B.juncea or B. rapa parental plants.

Assessment of Transfer of Dicamba Tolerance and Fertility of Hybrids Whole-plant screening was performed to determine the transfer of auxinic herbicide tolerance from S. arvensis into hybrids. Auxinic herbicide-R S. arvensis, B.juncea, and B. rapa, as well as hybrids were grown in a growth chamber (as described earlier). The seedlings were treated with dicamba, 200 g acid equivalents/hectare (ae/ha), at three to four leaf stage of development using a motorized hood sprayer. Dicamba was used because it is a widely used broadleaf weed herbicide and also S. arvensis biotypes were found highly resistant to dicamba. Heap and Morrison (1992). The sprayer was equipped with a flat-fan nozzle (8002 E) and calibrated to deliver 200 L/ha at 276 kPa. One and two weeks after treatment, the seedlings were visually rated for injury. The hybrid plants were classified as R or S by comparing the injury response with those of S. arvensis (dicamba-R) or B. juncea or B. rapa (dicamba-S) seedling response. Susceptibility of plants to dicamba was assessed based on epinasty symptoms (downward curling of leaf and stem tissue) followed by death; R plants were not affected by dicamba application. The fertility of the hybrids was tested by performing reciprocal crosses between hybrids and B.juncea or B. rapa.

Repeated Backcrosses to Introgress Dicamba Tolerance from Hybrids into B.juncea or B. rapa The first generation of backcross progeny (BCiFi) was produced by performing reciprocal crosses between dicamba-R hybrids and B. juncea or B. rapa. The hybrids that survived dicamba treatment, as well as B.juncea, and B. rapa were grown in a growth chamber (as described before). Cross-pollinations were performed between hybrids and B. juncea or B. rapa to obtain BCiFi seed. Jugulam et al. (2005). Mature seed (BCiFi) was harvested only from the cross between B.juncea hybrid. BCiFi seed of B.juncea x hybrid was raised in a growth chamber (as described previously). Because there was poor BCiFi production and germination, only three BCiFi seedlings were grown to maturity. These BCiFi seedlings were not screened for dicamba tolerance. Upon flowering, two of BCiFi plants were found male fertile because the pollen from these plants was able to produce BC2Fi seed when used as pollen parent. Up to 25

BC2Fiseeds were produced, and only 10 were germinated. When BC2Fi seedlings were at three to four leaf stage of development, they were treated with dicamba (200 g ae/ha).

Assessment of DNA Ploidy of Hybrids and Backcross Progeny The DNA ploidy of hybrids, and backcross progeny, was assessed by flow cytometry following the protocol described by Kron et al, (2007). Auxinic herbicide-R S. arvensis, B.juncea and B. rapa were used as controls. Based on DNA content obtained by flow cytometry, DNA ploidy of hybrids as well as backcross progeny was estimated.

Production of Hybrids between B.juncea or B. rapa and S. arvensis Cross-pollination between B. juncea, or B. rapa and dicamba-R S. arvensis produced siliques but failed to develop in vivo as they were unable to grow and eventually aborted. Ovule/embryo rescue from immature siliques facilitated hybrid plant production. More hybrids (up to 32) were produced in vitro in crosses between B. rapa x S. arvensis compared to B.juncea x S. arvensis (6 hybrids) (Table 36).

There was no significant difference in number of hybrids produced when embryos were cultured on either medium A or B. The hybrids exhibited several morphological traits of both the parents (e.g., leaf shape, stem, and plant height; Fig. 10). The clones of the hybrids were also successfully established in vitro by nodal cuttings.

Dicamba Tolerance in Hybrids and Backcross Progeny The results of whole plant screening demonstrated that one hybrid of B. juncea S. arvensis and 10 hybrids of B. rapa S. arvensis were dicamba-R. After treatment with dicamba, the dicamba-R hybrids exhibited little or no epinasty. This response was similar to dicamba-R S. arvensis plants. These plants grew and produced flowers (Fig. 11D and E). B.juncea, B. rapa as well as S hybrids died three weeks after dicamba treatment (Fig. 11A and B). The dicamba-R hybrids were maintained in vitro by nodal cuttings while the dicamba-S hybrids were discarded. Reciprocal crosses were performed between parents and dicamba-R hybrids to assess fertility of hybrids as well as generate backcross progeny. Crosses were successful only when B. juncea was used as a female parent. These results suggest that the sole dicamba-R hybrid derived from a cross between B.juncea and S. arvensis was only pollen fertile. All other crosses were not successful. About 15 BCiFi seeds were harvested from crosses between B.juncea and dicamba-R hybrid, and only three seed germinated. Ten seedlings of BC2Fi were established, and when treated with dicamba, the BC2Fi progeny showed varying responses. One week after dicamba treatment, six seedlings exhibited severe epinasty and eventually died. The remaining four plants showed minor dicamba injury initially and recovered from the symptoms. Chi-square tests were performed to determine the goodness of fit to a 1:1 (R:S) segregation, since tolerance to dicamba is determined by a single dominant gene in S. arvensis (Jugulam et al, 2005, Jasienuik et al, 1995). The null hypothesis was stated as "the observed frequencies of R and S upon dicamba treatment are in accordance with the expected frequencies for a 1:1 segregation." χ2 The progeny from BC2Fi showed 4:6 (R:S) segregation with a value of 0.4 (which is less than the table value of '3.841' at 0.05 probability for 1 degree of freedom). Therefore, the null hypothesis was accepted for observed frequencies of R and S plants in BC2Fi The 1:1 segregation of R:S in backcross 2 shows that the gene controlling dicamba tolerance was transferred to B.juncea. In the case of B. rapa S. arvensis hybrids, five out of ten dicamba-R hybrids also produced pollen. Assessment DNA Ploidy of Hybrids and Backcross Progeny Flow cytometry quantified the amount of DNA in hybrid, as well as in parental plants, and based on the amount, the DNA ploidy of the hybrid plants as well as backcross progeny was estimated. These data indicated that only one hybrid derived from B.juncea x S. arvensis was a DNA triploid (estimated DNA ploidy of 2.96; Table 37) while the five other hybrids were tetraploids (Table 37). These results show that the five hybrids that were DNA tetraploids (Table 37) were derived in vitro from somatic tissue rather than the fertile embryo. Moreover, all these hybrids were found to be dicamba-S. Because B. rapa was a diploid same as that of S. arvensis, although DNA was quantified by flow cytometry (Table 37), the hybridity of plants derived by embryo rescue could not be determined by flow cytometry data. The hybridity of B. rapa S. arvensis plants was ascertained by plant morphology as well as their tolerance response to dicamba treatment suggested they were hybrids. The flow cytometry results of BC1F1 and BC2F i progeny of B. juncea indicated that these plants were either DNA tetraploids (4 possibly somatic), or aneuploids (plants possessing abnormal number of chromosomes i.e., an extra or missing chromosome; e.g., 4.2x, 4.3*, 4.4x, 4.6* or 4.7*; Table 37). In intergeneric (or interspecific) crosses for transferring agronomically important traits, successful interspecific gene transfer encounters a number of barriers, specifically among genera and species having different genomes or different chromosome numbers, e.g., because of potential irregularities during meiosis etc. The family Brassicaceae consists of diploids (e.g., B. rapa, B. oleraceae, B. nigra, and S. arvensis etc.) as well as allotetraploids (e.g., B. juncea, B. napus, B. carinata). Successful production of hybrids and transfer of auxinic herbicide tolerance from S. arvensis to B.juncea S. arvensis hybrids was achieved. Specifically, the procedure of in vitro culturing of immature siliques 3-5 days after pollination for 2 weeks, followed by excision of embryos/ovules unexpectedly yielded higher number of hybrids in crosses between B. rapa x S. arvensis (32 hybrids) compared to B.juncea x S. arvensis (6 hybrids) (Table 36). These results offer a basis for further development of auxinic herbicide-R B. juncea and B. rapa oilseed crops. Creation of herbicide-resistant Brassica crops by this non-transgenic approach facilitates effective weed control, encourages less tillage, provides herbicide rotation options, minimizes occurrence of herbicide tolerance, and may have wider acceptance of these crops among growers across the globe. In addition, the presence of the herbicide-tolerance trait in different Brassica genomes offers the ability to more readily introgress the trait into other Brassica species and their derivatives, e.g., Raphanobrassica.

EXAMPLE 7 : Identification of S. arvensis Auxin-Herbicide Resistance Trait Genes (e.g., Dicamba-Tolerance). The full genomes of dicamba-herbicide-susceptible (dicamba-S) and dicamba-herbicide- tolerant (dicamba-R) Sinapis arvensis plants were sequenced and the genomic sequences were cross-compared for SNPs. Similarly, dicamba-herbicide-susceptible B. napus and introgressed dicamba-herbicide-tolerant B. napus plant genomes were sequenced and compared for SNPs. SNPs that occurred in coding sequences (CDSs) and resulted in an expressed amino acid substitution were identified and are shown in Table 38. The CDSs of these genes were annotated for the function of the encoded protein that either: (i) matched a protein listed as a dicamba- binding protein; or (ii) matched a protein listed as an auxin-signal or auxin-transport protein. The resulting group of genes that met either criterion (i) or criterion (ii) comprises the genes and translated polypeptides shown in Table 38. Based on the above analysis, each of the auxin herbicide tolerance trait (-R) genes identified in Table 38 was ascribed as independently providing dicamba herbicide tolerance, i.e. each of these genes encodes a DART auxin herbicide tolerance trait. Of the 18 amino-acid- altered, DART trait genes identified in this analysis, 16 were found to correspond to herbicide- susceptible genes. See Table 38, SEQ ID NOs:8, 12, 18, 22, 26, 30, 36, 40, 44, 48, 52, 56, 62, 66, 76, and 80 (Gene Nos. 1-2, 4-7, 9-21). For two of the identified 18 DART trait genes, no corresponding herbicide-susceptible gene has yet been identified. See Table 38, SEQ ID NOs:16 and 34 (i.e., Gene Nos. 3 and 8, respectively). Gene No. 15 (SEQ ID NO:58) has been assigned as an alternative dicamba-susceptible gene corresponding to DART trait Gene 14R (SEQ ID NO:56). Similarly, Gene Nos. 18, 19.1, and 19.2 (i.e. SEQ ID NOs:70, 72, and 74) were assigned as alternative dicamba-susceptible genes corresponding to DART trait gene 17R (SEQ ID NO:66). Table 38. S. arvensis Auxin-Herbicide Tolerance Trait Sequences (e.g., Dicamba-Tolerance). Chloroplast Trans- Membrane, Auxin- Sar_R_ 1119.g4334.tl SEQ ID NO:26 SEQ ID NO:27 6 R Associated Protein- 1 (cpTAAP-1) Chloroplast Trans- Membrane, Auxin- Sar_S_31217.g55588.tl SEQ ID NO:28 SEQ ID NO:29 6 S Associated Protein- 1 (cpTAAP-1) Endo-Mitochondrial, Sar_R_21396.g52442.tl Auxin-Associated Protein- 1 SEQ ID NO:30 SEQ ID NO:31 7 R (mtAAP-1) Endo-Mitochondrial, Sar_S_20600.g42686.tl Auxin-Associated Protein- 1 SEQ ID NO:32 SEQ ID NO:33 7 S (mtAAP-1) Zinc-binding ribosomal Sar_R_3394.gl2813.tl SEQ ID NO:34 SEQ ID NO:35 8 R* protein family protein SAUR-like auxin- Sar_R_4824.gl7686.tl responsive protein family SEQ ID NO:36 SEQ ID NO:37 9 R transcription factor SAUR-like auxin- Sar_S_12132.g28371.tl responsive protein family SEQ ID NO:38 SEQ ID NO:39 9 S transcription factor Sar_R_38470.g64923.tl HTA13 histone SEQ ID NO:40 SEQ ID NO:41 10 R

Sar_S_4108.gl0914.tl HTA13 histone SEQ ID NO:42 SEQ ID NO:43 10 S Knotted 1 like (KNAT4) Sar_R_706.G2840.Tl SEQ ID NO:44 SEQ ID NO:45 11 R transcription factor Knotted 1 like (KNAT4) Sar_S_5265.G13674.Tl SEQ ID NO:46 SEQ ID NO:47 11 S transcription factor Zinc-Finger-Like, Auxin- Sar_R_19858.g50467.tl Associated Protein- 1 SEQ ID NO:48 SEQ ID NO:49 12 R (zFAAP-1) Zinc-Finger-Like, Auxin- Sar_S_68453.g74218.tl Associated Protein- 1 SEQ ID NO:50 SEQ ID NO:51 12 S (zFAAP-1) Alba DNA/RNA-binding Sar_R_10390.g33598.tl SEQ ID NO:52 SEQ ID NO:53 13 R protein transcription factor Alba DNA/RNA-binding Sar_S_2341 1.g46486.tl SEQ ID NO:54 SEQ ID NO:55 13 S protein transcription factor Sar_R_241 1.G9430.Tl IAA16 transcription factor SEQ ID NO:56 SEQ ID NO:57 14 R

Sar_S_29291.G53554.Tl IAA16 transcription factor SEQ ID NO:58 SEQ ID NO:59 14 S

Sar_S_3202.G8615.Tl IAA16 transcription factor SEQ ID NO:60 SEQ ID NO:61 15 S 1 IAA12 Sar_R_21966.G53064.Tl bodenlos/monopteros SEQ ID NO:62 SEQ ID NO:63 16 R transcription factor Table 38. S. arvensis Auxin-Herbicide Tolerance Trait Sequences (e.g., Dicamba-Tolerance). IAA12 Sar S 12886.G29900.T1 bodenlos/monopteros SEQ ID NO: 64 SEQ ID NO:65 16 S Sar_S_59713.G71763.Tl transcription factor Sar_R_36336.g63877.tl RUB1 SEQ ID NO: 66 SEQ ID NO: 67 17 R

Sar_S_7171.gl8066.tl RUB1 SEQ ID NO:68 SEQ ID NO: 69 17 S 2

Sar_S_16564.g36270.tl RUB1 SEQ ID NO: 70 SEQ ID NO:71 18 S

Sar_S_19347.g4081 1.tl RUB1 SEQ ID NO: 72 SEQ ID NO: 73 19.1 S 2

Sar_S_23559.g46658.tl RUB1 SEQ ID NO: 74 SEQ ID NO: 75 19.2 S 2

Sar_R_16466.g45572.tl CAM7 transcription factor SEQ ID NO: 76 SEQ ID NO:77 20 R

Sar_S_29545.g53829.tl CAM7 transcription factor SEQ ID NO:78 SEQ ID NO:79 20 S cytochrome P450 Sar_R_3970.gl4672.tl SEQ ID NO:80 SEQ ID NO:81 2 1 R CYP83A1 cytochrome P450 Sar_S_51371.g68743.tl SEQ ID NO: 82 SEQ ID NO:83 2 1 S CYP83A1 * No dicamba susceptible counterpart gene yet identified. 1 15S is a second dicamba susceptible gene corresponds to resistant gene 14R. 2 Genes 18, 19.1, and 19.2 are three additional dicamba susceptible genes correspond to resistant gene 17R.

Transformation of Arabidopsis Plants The 18 dicamba resistant, DART trait genes listed in Table 38 were cloned from dicamba-R S. arvensis and used to construct Arabidopsis expression vectors (i.e. the 'R' genes among Gene Nos. 1-21, as shown in Table 38). The herbicide susceptible genes corresponding to the herbicide resistant genes were cloned from dicamba-S S. arvensis and used to construct Arabidopsis expression vectors (i.e., the 'S' genes among Gene Nos. 1-21, as shown in Table 38). The expression vectors were designed for over-expression of these genes. Each of the resulting expression vectors was used to transform A. thaliana plants. Three groups of each of the transformed plants are sprayed with 50 g ai/ha of dicamba or aminopyralid or 2,4-D. At 2 weeks after treatment, the degree of auxinic herbicide tolerance provided by each DART trait is scored based on the amount of phytotoxicity exhibited by the transformed plants. 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What is claimed is:

1. A non-transgenic auxinic herbicide tolerant Brassica crop plant, or plant part thereof, comprising the auxinic herbicide-tolerance characteristic of any of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent Deposit Designation

Numbers PTA-120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA-1 1213, and PTA- 11214, respectively, with the proviso that the Brassica plant is of a species other than Sinapis arvensis; and wherein the auxinic herbicide tolerance is greater than that exhibited by a wild type variety of said plant lacking said auxinic herbicide tolerance.

2. The plant of claim 1, wherein the plant is a Brassicajuncea, B. napus, or B. rapa plant.

3. The plant of claim 1 or 2, wherein the plant is a canola plant.

4. A descendant of the plant of any one of claims 1-3 wherein the descendant has the auxinic herbicide-tolerance characteristic.

5. A seed of or capable of producing a plant according to any one of claims 1-4.

6. The seed according to claim 5, wherein the seed has, disposed on a surface thereof, a composition comprising at least one agronomically acceptable ingredient.

7. The seed according to claim 5 or 6, wherein said ingredient is at least one agronomically acceptable herbicide, fungicide, nematicide, or insecticide, or a combination thereof. 8. The seed according to claim 7, wherein said insecticide comprises at least one anti- coleopteran agent, anti-hemipteran agent, anti-lepidopteran agent, or a combination thereof.

9. A method for treating the seed of claim 5 with an agronomically acceptable composition, the method comprising contacting the agronomically acceptable composition with the seed, wherein the composition comprises an auxinic herbicide.

10. A method for preparing a treated seed comprising providing a seed according to claim 5 and applying thereto a seed treatment composition.

11. A cell of the plant according to claim 1.

12. A plant product produced from the plant according to claim1.

13. The plant product of claim 12, wherein the plant product is grain, meal, or oil.

14. A method for controlling weeds in a field by application of an auxinic herbicide without significantly inhibiting the growth of a Brassica plant, the method comprising: (a) providing a Brassica plant or seed of any one of claims 1-5; and (b) applying an herbicide composition comprising an effective amount of an auxinic herbicide: (i) to the field, followed by planting of said plant or seed in therein; (ii) to the field, during or after planting of said seed therein; (iii) to the plant in said field and to weeds in the vicinity of the plant; or (iv) to said seed, followed by planting of said seed in the field; wherein the effective amount of the auxinic herbicide would significantly inhibit the growth of a corresponding wild-type variety of said Brassica plant; thereby controlling weeds. 15. The method of claims 14, wherein the plant is a Brassica juncea, B. napus, or B. rapa plant.

16. The method of claim 14 or 15, wherein the plant is a canola plant.

17. The method of any one of claims 14-16, wherein the step of applying comprises performing post-emergent treatment of the plant by applying an herbicide composition, comprising auxinic herbicide(s), to the plant and its immediate vicinity, at a dose rate of about 10 to about 5000 g/ha active ingredient (A.I.).

18. The method of any one of claims 14-17, wherein the step of applying comprises performing pre-emergent treatment, or 0 to 30 days-pre-planting treatment, of the plant by applying an herbicide composition, comprising auxinic herbicide(s), to the seed planting locus thereof and its immediate vicinity, at a dose rate of about 10 to about 5000 g/ha A.I.

19. A method for producing an auxinic herbicide-tolerant progeny plant, the method comprising: (a) providing a parent plant of a desired line; and (b) crossing the parent plant with any one of the plants according of claims 1-4 to introduce the auxinic herbicide-tolerance characteristic into the germplasm of the progeny plant, wherein the progeny plant thereby has increased tolerance to an auxinic herbicide relative to the parent plant.

20. The method of claim 19 further comprising: introgressing the auxinic herbicide-tolerance characteristic of the progeny plant through traditional plant breeding techniques to obtain a descendent plant having the auxinic herbicide-tolerance characteristic.

The method of claim 19 or 20, wherein the parent plant comprises at least one herbicide tolerant (HT) mutant AHASL gene. 22. The method of any one of claims 19-21, wherein the parent plant is a dicot.

23. The method of any one of claims 19-22, wherein the parent plant is a Brassica or Rhaphanus plant.

24. The method of any one of claims 19-23, wherein the parent plant is a Brassicajuncea, B. napus, or B. rapa plant.

25. The method of any one of claims 19-24, wherein the parent plant is a canola plant.

26. A plant produced by the method of claim 19 or 20.

27. A descendant of the plant of claim 26, wherein the descendant has the auxinic herbicide- tolerance characteristic.

28. The plant of any of claims 19 and 20, wherein the plant is non-transgenic.

29. A method for treating the plant of any one of claims 1-4, 12, and 26-28 with an agronomically acceptable composition, the method comprising contacting the plant with the agronomically acceptable composition.

30. Method of claim 29, wherein the composition comprises an auxinic herbicide.

31. The method of claim 30, wherein the composition further comprises a fungicide, an insecticide, or a nematicide.

32. A method for preparing a descendant seed, the method comprising planting a seed of claim 5.

33. The method of claim 32, further comprising harvesting seed from said plant. 34. The method of claim 32 or 33 further comprising applying an auxinic herbicidal composition to the descendent plant.

35. A method for producing a plant product from the plant of any one of claims 1-4, 12, and 26-28, the method comprising processing the plant or a plant part thereof to obtain the plant product.

36. The method of claim 35, wherein the plant product is fodder, seed meal, oil, or seed- treatment-coated seeds.

37. The method of claim 35 or 36, wherein the plant part is a seed.

38. The plant of claim 1, wherein the plant is a descendent of a member of any one of said lines.

39. The plant of claim 38, wherein the descendant was obtained by traditional plant breeding from said member.

40. The plant of any one of claims 1-4, 12, and 26-28, wherein said plant shows no significant visible injury 36 days after treatment with dicamba at the 2-3 leaf stage, at a rate equivalent to 240 g/ha dicamba A.I.

41. Nucleic acid comprising: (a) a chimeric polynucleotide comprising both a Sinapis arvensis polynucleotide portion and a Brassica polynucleotide portion, wherein said chimeric polynucleotide encodes the DART trait of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent Deposit Designation Numbers PTA-120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA- 11213, and PTA-1 1214, respectively; or (b) a mutagenized or recombinant polynucleotide encoding the DART trait of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with the ATCC under Patent Deposit

Designation Numbers PTA-120132, PTA-1 121 1, PTA-12050, PTA-1 1212, PTA- 11213, and PTA-1 1214, respectively.

42. The nucleic acid of claim 41, wherein the DART trait is encoded by the Sinapis arvensis polynucleotide.

43. The nucleic acid of claim 41, wherein the nucleic acid is isolated.

44. The nucleic acid of any one of claims 41-43 operably linked to a promoter operable in plant cells.

45. A plant, or plant part thereof, comprising the nucleic acid of claim 4 1 and expressing the DART trait therefrom.

46. The plant or plant part of claim 45, wherein the plant is nontransgenic.

47. The plant or plant part of claim 45, wherein the plant is a Brassica or Raphanobrassica.

48. A plant product produced from the plant according to claim 45.

49. The plant product of claim 48, wherein the plant product is grain, meal, or oil.

50. A seed comprising the nucleic acid of claim 41, wherein the seed is capable of expressing the DART trait therefrom. 51. The seed of claim 50, wherein the seed is treated with a seed treatment composition optionally comprising an auxinic herbicide.

52. The seed of claim 50, wherein the seed is a Brassica or Raphanobrassica.

53. The seed of claim 50, wherein the seed is nontransgenic.

54. A cell comprising the nucleic acid of claim 4 1 and capable of expressing the DART trait therefrom.

55. The cell of claim 54, wherein the cell is a plant cell.

56. The cell of claim 54, wherein the cell is a Brassica or Raphanobrassica.

57. The cell of claim 54, wherein the cell is nontransgenic.

58. The plant, seed, or cell of any one of claims 45, 50, or 54, respectively, wherein the plant, seed, or cell expresses the DART trait and, as a result, is tolerant to an auxinic herbicide.

59. A method for controlling weeds in a field by an application of an auxinic herbicide without significantly inhibiting the growth of a crop plant, the method comprising: (a) providing a crop plant or seed or said crop plant comprising the nucleic acid of claim 41, the expression of said nucleic acid conferring to the plant or seed tolerance to an auxinic herbicide; and (b) applying an herbicide composition comprising an effective amount of an auxinic herbicide: (i) to the field, followed by planting of said plant or seed in therein; (ii) to the field, during or after planting of said seed therein; (iii) to the plant in said field and to weeds in the vicinity of the plant; (iv) to said seed, followed by planting of said seed in the field; or (v) to a plant by the seed after it has been planted in the field, and to weeds in the vicinity of the plant; wherein said effective amount of said auxinic herbicide would significantly inhibit the growth of a corresponding wild-type variety of said crop plant; thereby controlling weeds.

60. A method for controlling weeds in a crop field by use of an auxinic herbicide without significantly inhibiting the growth of a crop plant, the method comprising: (a) providing a seed-treatment-treated seed comprising the nucleic acid of claim 41, the expression of said nucleic acid conferring to the plant or seed tolerance to an auxinic herbicide, the seed treatment comprising an auxinic herbicide; and (b) planting said treated seed in the field.

6 1. The method of claim 59 or 60, wherein the crop is a Brassica or Raphanobrassica crop.

62. The method of claim 61, wherein the Brassica is B. napus, B.juncea, or B. rapa.

63. The method of claim 59 or 60, wherein the crop is nontransgenic.

64. The method of any one of claims 59-63, wherein the auxinic herbicide composition comprises at least one of clomeprop; cloprop ("3-CPA"); 4-chlorophenoxyacetic acid ("4-CPA"); 2-(4-chlorophenoxy)propionic acid ("4-CPP"); 2,4-dichlorophenoxy acetic acid ("2,4-D"); (3,4-dichlorophenoxy)acetic acid ("3,4-DA"); 4-(2,4- dichlorophenoxy)butyric acid ("2,4-DB"); 2-(3,4-dichlorophenoxy)propionic acid ("3,4- DP"); tris[2-(2,4-dichlorophenoxy)ethyl]phosphite ("2,4-DEP"); dichlorprop ("2,4-DP"); 2,4,5-trichlorophenoxyacetic acid ("2,4,5-T"); fenoprop ("2,4,5-TP"); 2-(4-chloro-2- methylphenoxy)acetic acid ("MCPA"); 4-(4-chloro-2-methylphenoxy)butyric acid ("MCPB"); mecoprop ("MCPP"); chloramben; dicamba; tricamba; 2,3,6-trichlorobenzoic acid ("TBA"); aminopyralid; clopyralid; fluroxypyr; picloram; triclopyr; quinclorac; quinmerac; or benazolin. 65. The method of any one of claims 59-63, wherein the auxinic herbicide composition comprises at least one of clomeprop; cloprop ("3-CPA"); 4-chlorophenoxyacetic acid ("4-CPA"); 2-(4-chlorophenoxy)propionic acid ("4-CPP"); (3,4-dichlorophenoxy)acetic acid ("3,4-DA"); 4-(2,4-dichlorophenoxy)butyric acid ("2,4-DB"); 2-(3,4- dichlorophenoxy)propionic acid ("3,4-DP"); tris[2-(2,4-dichlorophenoxy)ethyl]phosphite ("2,4-DEP"); dichlorprop ("2,4-DP"); 2,4,5-trichlorophenoxyacetic acid ("2,4,5-T"); fenoprop ("2,4,5-TP"); 4-(4-chloro-2-methylphenoxy)butyric acid ("MCPB"); chloramben; dicamba; tricamba; 2,3,6-trichlorobenzoic acid ("TBA"); aminopyralid; clopyralid; picloram; quinclorac; quinmerac; or benazolin.

66. The method of any one of claims 59-63, wherein the auxinic herbicide composition comprises at least one of: 4-(2,4-dichlorophenoxy)butyric acid ("2,4-DB"); dicamba; aminopyralid; picloram; or quinclorac.

67. The method of any one of claims 59-63, wherein the auxinic herbicide composition comprises at least one of: aminopyralid or picloram.

68. The method of any one of claims 59-63, wherein the auxinic herbicide composition comprises dicamba.

69. A method for treating a seed comprising: (a) providing a seed comprising the nucleic acid of claim 41, the expression of the nucleic acid conferring to the plant tolerance to an auxinic herbicide; and (b) contacting said seed with an agronomically acceptable composition.

70. The method of claim 69, wherein the agronomically acceptable composition comprises an auxinic herbicide.

71. The method of claim 69, wherein the agronomically acceptable composition further comprises a fungicide, an insecticide, a nematicide, or a further herbicide. 72. A method for preparing a seed, the method comprising: growing a plant comprising the nucleic acid of claim 41, the expression of the nucleic acid conferring to the plant tolerance to an auxinic herbicide.

73. The method of claim 72, further comprising harvesting seed from said plant.

74. The method of claim 72, further comprising applying auxinic herbicide.

75. A method for producing a plant product from a plant comprising the nucleic acid of claim 41, or plant part thereof, the method comprising processing the plant, or a plant part thereof to obtain the plant product.

76. The method of claim 75, wherein the plant product is grain, fodder, meal, oil, or seed- treatment-coated seeds.

77. The method of claim 75, wherein the plant part is a seed.

78. A method of producing a progeny plant having tolerance to an auxinic herbicide, the method comprising crossing a first auxinic herbicide-tolerant plant with a second plant to produce an auxinic herbicide-tolerant progeny plant, wherein the first plant is the plant of claim 45.

79. The method according to any one of claims 69, 72, 75, or 78, wherein the seed of claim 69 or 72, or the plant according to claim 75 or the first plant according to claim 78, is a Brassica or Raphanobrassica seed or plant, respectively.

80. The method of claim 79, wherein the seed or plant is non-transgenic.

81. A method for identifying an auxinic herbicide tolerant plant, or plant part thereof, the method comprising: (a) providing biological material from a plant comprising the DART trait; (b) performing PCR, hybridization testing, or sequencing of said nucleic acid in said biological material to determine if said plant comprises the DART trait; and (c) identifying, based on the results of step (b), that the plant comprises the DART trait.

82. The method of claim 81, wherein the plant comprises: (i) any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13-l-18, DT-01 SA2-R, or DT-01 BC8SA2-R, a representative sample of seed of each line having been deposited with American Type Culture Collection (ATCC) under Patent Deposit Designation Numbers PTA-120132,

PTA-1 121 1, PTA- 12050, PTA-1 1212, PTA-1 1213, and PTA-1 1214, respectively; (ii) a mutant, recombinant, or a genetically engineered derivative of any one of lines DT-01 Cyc2 BNS4, DT-01 BC3Bn#13, DT-01 BC4Bn#13-l, DT-01 BC5Bn#13- 1-18, DT-01 SA2-R, or DT-01 BC8SA2-R and that expressed the DART trait thereof; or (iii) a plant that is a progeny of at least any one of the plants of (i)-(ii) and that expressed the DART trait thereof.

83. The method of claim 8 1 or 82, wherein the biological material comprises a Brassica or a Raphanobrassica plant, plant part thereof, seed, or cell.

84. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) the nucleotide sequence of SEQ ID NO: 8; (b) a nucleotide sequence encoding a translation elongation factor EF1A/initiation factor family polypeptide comprising the amino acid sequence of SEQ ID NO:9, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO: 8 and encodes an auxinic-herbicide-tolerant, functional, translation elongation factor EF1A/initiation factor family polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, translation elongation factor EF1A/initiation factor family polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:9.

85. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) the nucleotide sequence of SEQ ID NO: 12; (b) a nucleotide sequence encoding a KAT2 peroxisomal 3-ketoacyl-CoA thiolase 3 polypeptide comprising an amino acid sequence according to SEQ ID NO: 13, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO: 12 and encodes an auxinic-herbicide-tolerant, functional, KAT2 peroxisomal 3-ketoacyl- CoA thiolase 3 polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, KAT2 peroxisomal 3-ketoacyl-CoA thiolase 3 polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO: 13.

86. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO: 16; (b) a nucleotide sequence encoding a calmodulin 5-like polypeptide comprising an amino acid sequence according to SEQ ID NO: 17, or a mature form thereof

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO: 16 and encodes an auxinic-herbicide-tolerant, functional, calmodulin 5-like polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional,

calmodulin 5-like polypeptide comprising an amino acid sequence at least 70%> identical to SEQ ID NO: 17.

87. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO: 18; (b) a nucleotide sequence encoding an ARF21 transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO: 19, or a mature form thereof (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO: 18 and encodes an auxinic-herbicide-tolerant, functional, ARF21 transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, ARF21 transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO: 19.

88. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:22; (b) a nucleotide sequence encoding a TPR3 transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:23, or a mature form thereof;

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:22 and encodes an auxinic-herbicide-tolerant, functional, TPR3 transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, TPR3 transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:23.

89. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:26; (b) a nucleotide sequence encoding a Chloroplast Trans-Membrane, Auxin- Associated Protein- 1 (cpTAAP-1) polypeptide comprising an amino acid sequence according to SEQ ID NO:27, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:26 and encodes an auxinic-herbicide-tolerant, functional, Chloroplast Trans-Membrane, Auxin-Associated Protein- 1 (cpTAAP-1) polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, Chloroplast Trans-Membrane, Auxin-Associated Protein- 1 (cpTAAP-1) polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:27.

The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:30; (b) a nucleotide sequence encoding an Endo-Mitochondrial, Auxin-Associated Protein- 1 (mtAAP-1) polypeptide comprising an amino acid sequence according to SEQ ID NO:31, or a mature form thereof;

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:30 and encodes an auxinic-herbicide-tolerant, functional, Endo-Mitochondrial, Auxin- Associated Protein-1 (mtAAP-1) polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, Endo- Mitochondrial, Auxin-Associated Protein-1 (mtAAP-1) polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:31.

The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:34; (b) a nucleotide sequence encoding a zinc-binding ribosomal protein family polypeptide comprising an amino acid sequence according to SEQ ID NO:35, or a mature form thereof;

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:34 and encodes an auxinic-herbicide-tolerant, functional, zinc-binding ribosomal protein family polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, zinc- binding ribosomal protein family polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:35.

92. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:36; (b) a nucleotide sequence encoding a SAUR-like auxin-responsive protein family transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:37, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:36 and encodes an auxinic-herbicide-tolerant, functional, SAUR-like auxin-responsive protein family transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, SAUR- like auxin-responsive protein family transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:37.

93. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:40; (b) a nucleotide sequence encoding a HTA13 histone polypeptide comprising an amino acid sequence according to SEQ ID NO:41, or a mature form thereof;

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:40 and encodes an auxinic-herbicide-tolerant, functional, HTA13 histone polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, HTA13

histone polypeptide comprising an amino acid sequence at least 70%> identical to SEQ ID NO:41.

94. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:44; (b) a nucleotide sequence encoding a Knotted 1 like (KNAT4) transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:45, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:44 and encodes an auxinic-herbicide-tolerant, functional, Knotted 1 like (KNAT4) transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, Knotted 1 like (KNAT4) transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:45.

95. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:48; (b) a nucleotide sequence encoding a Zinc-Finger-Like, Auxin-Associated Protein-1 (zFAAP-1) polypeptide comprising an amino acid sequence according to SEQ ID NO:49, or a mature form thereof;

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:48 and encodes an auxinic-herbicide-tolerant, functional, Zinc-Finger-Like, Auxin- Associated Protein- 1 (zFAAP-1) polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, Zinc- Finger-Like, Auxin-Associated Protein- 1 (zFAAP-1) polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:49.

96. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:52; (b) a nucleotide sequence encoding an Alba DNA/RNA-binding protein transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:53, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:52 and encodes an auxinic-herbicide-tolerant, functional, Alba DNA/RNA-binding protein transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, Alba DNA/RNA-binding protein transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:53.

The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:56; (b) a nucleotide sequence encoding an IAA16 transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:57, or a mature form thereof;

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:56 and encodes an auxinic-herbicide-tolerant, functional, IAA16 transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, IAA16 transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:57.

The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:62; (b) a nucleotide sequence encoding an IAA12 bodenlos/monopteros transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:63, or a mature form thereof;

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:62 and encodes an auxinic-herbicide-tolerant, functional, IAA12 bodenlos/monopteros transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, IAA12 bodenlos/monopteros transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:63.

99. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:66; (b) a nucleotide sequence encoding an RUB1 polypeptide comprising an amino acid sequence according to SEQ ID NO:67, or a mature form thereof; (c) a nucleotide sequence that is at least 90% homologous to SEQ ID NO:66 and encodes an auxinic-herbicide-tolerant, functional, RUB1 polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, RUB1

polypeptide comprising an amino acid sequence at least 70%> identical to SEQ ID NO:67.

100. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO:76; (b) a nucleotide sequence encoding a CAM7 transcription factor polypeptide comprising an amino acid sequence according to SEQ ID NO:77, or a mature form thereof;

(c) a nucleotide sequence that is at least 90%> homologous to SEQ ID NO:76 and encodes an auxinic-herbicide-tolerant, functional, CAM7 transcription factor polypeptide; or (d) a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, CAM7 transcription factor polypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:77.

101. The nucleic acid, plant, plant part, seed, or cell according to any one of claims 41-59, or the method according to any one of claims 60-83, wherein the DART trait comprises: (a) a nucleotide sequence according to SEQ ID NO: 80; a nucleotide sequence encoding a cytochrome P450 CYP83A1 polypeptide comprising an amino acid sequence according to SEQ ID NO:81, or a mature form thereof; a nucleotide sequence that is at least 90% homologous to SEQ ID NO: 80 and encodes an auxinic-herbicide-tolerant, functional, cytochrome P450 CYP83A1 polypeptide; or a nucleotide sequence encoding an auxinic-herbicide-tolerant, functional, cytochrome P450 CYP83Alpolypeptide comprising an amino acid sequence at least 70% identical to SEQ ID NO:81.