NeoBiotechnologies, Inc. 2 Union Square Union City, CA 94587 Tel: 510-376-5603 Email: [email protected] , [email protected] Website: www.NeoBiotechnologies.com

UACA / Nucling (Nuclear Membrane Marker) Mouse Monoclonal Antibody [Clone UACA/1222]

Catalog No Format Size Price (USD)

55075-MSM2-P0 Purified Ab with BSA and Azide at 200ug/ml 20 ug 199.00

55075-MSM2-P1 Purified Ab with BSA and Azide at 200ug/ml 100 ug 459.00

55075-MSM2-P1ABX Purified Ab WITHOUT BSA and Azide at 1.0mg/ml 100 ug 459.00

Human ID 55075 Immunogen Recombinant human UACA

Human SwissProt Q9ZF9 Host / Ig Isotype Mouse / IgG1, kappa

Human Unigene 108049 Mol. Weight of Antigen 160kDa

Human Gene Symbol UACA Cellular Localization Nuclear membrane

Human 15q22-q24 Species Reactivity Human. Mouse. Location Positive Control HeLa or 293T cells. Highly expressed in skeletal muscle, Synonyms Nuclear membrane binding protein; Uveal autoantigen with heart, kidney and pancreas. Also expressed in epidermal coiled-coil domains and ankyrin repeats (UACA); NUCLING melanocytes, eye muscles and thyroid follicular cells.

Western Blot of A549 cell lysate using UACA / Nucling Monoclonal Antibody (UACA/1222)

Specificity & Comments Supplied As UACA (Uveal Autoantigen with Coiled-coil domains and Ankyrin repeats) is 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. a 1,416 amino acid nuclear membrane protein. It was originally identified as Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available an autoantigen in patients with panuveitis, a characteristic of WITHOUT BSA & azide at 1.0mg/ml. Vogt-Koyanagi-Harada disease, and in patients with Graves' disease. UACA was also later identified as Nucling, an mRNA differentially expressed in F9 Storage and Stability embryonal carcinoma cells during cardiac muscle differentiation. UACA appears to function as a pro-apoptotic protein that recruits the Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 apaf-1-pro-caspase-9 complex for the induction of apoptosis to mediate the to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS cell-death pathway. required.

Known Applications & Suggested Dilutions Limitations This antibody is available for research use only and is not approved for use in diagnosis.

Western Blot (1-2ug/ml) Optimal dilution for a specific application should be determined. Warranty There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic Key References loss resulting from this product.

1. Ohkura, T., et al. 2004. Detection of the novel autoantibody (anti-UACA antibody) in patients with Graves disease. Biochem. Biophys. Res. Commun. 321: 432-440. 2. Yamada, K., et al. 2001. Identification of a novel autoantigen UACA in patients with panuveitis. Biochem. Biophys. Res. Commun. 280: 1169-1176.

NBT01222