W&M ScholarWorks
Presentations
10-9-2015
Integrative Taxonomy, a New Tool for Fisheries Conservation
Adela Roa-Varon Virginia Institute of Marine Science
Eric J. Hilton Virginia Institute of Marine Science
Follow this and additional works at: https://scholarworks.wm.edu/presentations
Part of the Aquaculture and Fisheries Commons, Biodiversity Commons, and the Zoology Commons
Recommended Citation Roa-Varon, Adela and Hilton, Eric J.. "Integrative Taxonomy, a New Tool for Fisheries Conservation". 10-9-2015. VIMS 75th Anniversary Alumni Research Symposium.
This Presentation is brought to you for free and open access by W&M ScholarWorks. It has been accepted for inclusion in Presentations by an authorized administrator of W&M ScholarWorks. For more information, please contact [email protected]. Integra ve Taxonomy: a New Tool for Fisheries Conserva on Adela Roa-Varon and Eric Hilton Virginia Ins tute of Marine Science, William & Mary College [email protected], [email protected]
What is Integra ve Taxonomy? The Process of Integra ve Results to Date Glossary Taxonomy Baits libraries were designed based on Species play a central role in nearly all candidate single-copy markers for disciplines of biology. Therefore delimita on Gadus morhua genome, using the Species are the fundamental of species has broad implica ons for efforts taxonomic unit for a wide array of MYBaits target enrichment system. A biological studies and applied fields ranging from biological conserva on to preliminary analysis was run to test the such as conserva on planning. compara ve evolu onary analyses. The rise of baits using Merluccius bilinearis and new genomic and bioinforma cs tools led for Gadus morhua as a posi ve control. Species delimita on: the process of species delimita on is becoming increasingly Approximately, 1500 to 3000 target iden fying species-level biological objec ve and integra ve. Gene capture in single-copy gene markers were diversity from empirical data. combina on with Next genera on iden fied. A er corrobora ng the Integra ve Taxonomy an approach Global distribu on of Merluccius spp. Sequencing (NGS) approaches allow collec on efficiency of the molecular technique, to taxonomic research that aims to of 1000s of loci for 100s of individuals. These 70 species were sequenced, including 23 incorporate the diverse data types new approaches also allow integra on of hakes represen ng all the species with and methods used in systema c gene c and non-gene c data in a unified biology to document biodiversity and Molecular Data excep on of Merluccius gayi-peruanus. framework. the evolu onary processes that Morphological data (meris c and morphometric data and internal Data is in the early stages of analysis promote divergence. morphology) are collected for at least 25 specimens from museum and at least 40 more samples are We use integra ve taxonomy to provide a specimens and voucher specimens from our molecular sampling. Hakes (Merluccius, Merlucciidae) are Type specimens will be examined. expected to be sequenced represen ng more rigorous species delimita on by all the species in the genus. some of the most important incorpora ng morphological and molecular Morphological data are being collected commercially harvested fishes in the data. Using hakes (Merlucciidae), an Genome-wide Gene c Data world. Iden fying their species and analyzed. This study will result in a remains problema c due to similar ecologically and commercially important be er understanding of the evolu on of morphology across species, which family of fishes, as a model, this study aims to: commercially valuable fishes, which is results in difficul es in assessing necessary for effec ve fisheries species distribu ons and stocks. 1) Iden fy species using integra ve management and conserva on. taxonomy Gene Capture or DNA hybridiza on Reference allows for hundreds of pre-specified 2) Reconstruct their phylogene c Li, C., Hofreiter, M., Straube, N., Corrigan, S., genes to be targeted and isolated for rela onships using a mul species Naylor, G.J.P. 2013. Capturing protein-coding sequencing in a single experiment. coalescent approach. DNA sequences from single-copy protein-coding genes shared genes across highly divergent species. across hakes were targeted using the gene capture method BioTechniques 54:321–26 Next Genera on Sequencing (NGS) 3) Inves gate the global biogeography and developed by Li et al (2013). also known as high-throughput diversifica on of hakes. Acknowledgments Expected Results sequencing, is the term used to We thank Dr. Chenhong Li for training ARV in 1) Phylogeny of the family describe a number of different the molecular technique at Shanghai Ocean Due to the drama c declines in abundance Merlucciidae modern sequencing technologies University. Funding was provided by the and commercial landings, preserving the including: Illumina, Roche 454, Ion 2) Species delimita on of Na onal Science Founda on (NSF-EAPSI), Merlucccius torrent and SOLiD sequencing. biodiversity requires precise knowledge of the Student Research Grant and Associate Dean of taxonomy of hakes. 3) Biogeography of hakes Academic Studies at VIMS, and EJH research grants.