Mosquito Surveillance 2020
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Mosquito Species Identification Using Convolutional Neural Networks With
www.nature.com/scientificreports OPEN Mosquito species identifcation using convolutional neural networks with a multitiered ensemble model for novel species detection Adam Goodwin1,2*, Sanket Padmanabhan1,2, Sanchit Hira2,3, Margaret Glancey1,2, Monet Slinowsky2, Rakhil Immidisetti2,3, Laura Scavo2, Jewell Brey2, Bala Murali Manoghar Sai Sudhakar1, Tristan Ford1,2, Collyn Heier2, Yvonne‑Marie Linton4,5,6, David B. Pecor4,5,6, Laura Caicedo‑Quiroga4,5,6 & Soumyadipta Acharya2* With over 3500 mosquito species described, accurate species identifcation of the few implicated in disease transmission is critical to mosquito borne disease mitigation. Yet this task is hindered by limited global taxonomic expertise and specimen damage consistent across common capture methods. Convolutional neural networks (CNNs) are promising with limited sets of species, but image database requirements restrict practical implementation. Using an image database of 2696 specimens from 67 mosquito species, we address the practical open‑set problem with a detection algorithm for novel species. Closed‑set classifcation of 16 known species achieved 97.04 ± 0.87% accuracy independently, and 89.07 ± 5.58% when cascaded with novelty detection. Closed‑set classifcation of 39 species produces a macro F1‑score of 86.07 ± 1.81%. This demonstrates an accurate, scalable, and practical computer vision solution to identify wild‑caught mosquitoes for implementation in biosurveillance and targeted vector control programs, without the need for extensive image database development for each new target region. Mosquitoes are one of the deadliest animals in the world, infecting between 250–500 million people every year with a wide range of fatal or debilitating diseases, including malaria, dengue, chikungunya, Zika and West Nile Virus1. -
Caracterização Molecular Do Isolado Viral AR115: Evidência Da Circulação De Vírus Do Sorogrupo Gamboa No Sudeste Do Brasil
14 INSTITUTO EVANDRO CHAGAS NÚCLEO DE ENSINO E PÓS-GRADUAÇÃO PROGRAMA DE PÓS-GRADUAÇÃO EM VIROLOGIA Aline Gonçalves da Costa Caracterização molecular do isolado viral AR115: evidência da circulação de vírus do sorogrupo Gamboa no sudeste do Brasil ANANINDEUA 2018 15 Aline Gonçalves da Costa Caracterização molecular do isolado viral AR115: evidência da circulação de vírus do sorogrupo Gamboa no sudeste do Brasil Dissertação apresentada ao Programa de Pós- Graduação em Virologia do Instituto Evandro Chagas, para obtenção do título de Mestre em Virologia Orientadora: Prof.ª Dr.ª Ana Cecília Ribeiro Cruz ANANINDEUA 2018 16 Dados Internacionais de Catalogação na Publicação (CIP) Biblioteca do Instituto Evandro Chagas Costa, Aline Gonçalves da. Caracterização molecular do isolado viral AR115: evidência da circulação de vírus do sorogrupo Gamboa no sudeste do Brasil./ Aline Gonçalves da Costa. – Ananindeua, 2018. 54 f.: il.; 30 cm Orientadora: Dra. Ana Cecília Ribeiro Cruz Dissertação (Mestrado em Virologia) – Instituto Evandro Chagas, Programa de Pós-Graduação em Virologia, 2018. 1. Classificação. 2. Arbovírus. 3. Ortobunyavírus. 4. Artropodes. I. Cruz, Ana Cecília Ribeiro, orient. II. Instituto Evandro Chagas. III. Título. CDD: 579.2562 17 Aline Gonçalves da Costa Caracterização molecular do isolado viral AR115: evidência da circulação de vírus do sorogrupo Gamboa no sudeste do Brasil Dissertação apresentada ao Programa de Pós- Graduação em Virologia do Instituto Evandro Chagas, para obtenção do título de Mestre em Virologia Aprovado em: 10/01/2018 BANCA EXAMINADORA Profa. Dra. Daniele Barbosa de Almeida Medeiros Instituto Evandro Chagas Prof. Dr. Carlos Alberto Marques de Carvalho Instituto Evandro Chagas Dra. Adriana Ribeiro Carneiro Folador Universidade Federal do Pará Dr. -
Wild Species 2010 the GENERAL STATUS of SPECIES in CANADA
Wild Species 2010 THE GENERAL STATUS OF SPECIES IN CANADA Canadian Endangered Species Conservation Council National General Status Working Group This report is a product from the collaboration of all provincial and territorial governments in Canada, and of the federal government. Canadian Endangered Species Conservation Council (CESCC). 2011. Wild Species 2010: The General Status of Species in Canada. National General Status Working Group: 302 pp. Available in French under title: Espèces sauvages 2010: La situation générale des espèces au Canada. ii Abstract Wild Species 2010 is the third report of the series after 2000 and 2005. The aim of the Wild Species series is to provide an overview on which species occur in Canada, in which provinces, territories or ocean regions they occur, and what is their status. Each species assessed in this report received a rank among the following categories: Extinct (0.2), Extirpated (0.1), At Risk (1), May Be At Risk (2), Sensitive (3), Secure (4), Undetermined (5), Not Assessed (6), Exotic (7) or Accidental (8). In the 2010 report, 11 950 species were assessed. Many taxonomic groups that were first assessed in the previous Wild Species reports were reassessed, such as vascular plants, freshwater mussels, odonates, butterflies, crayfishes, amphibians, reptiles, birds and mammals. Other taxonomic groups are assessed for the first time in the Wild Species 2010 report, namely lichens, mosses, spiders, predaceous diving beetles, ground beetles (including the reassessment of tiger beetles), lady beetles, bumblebees, black flies, horse flies, mosquitoes, and some selected macromoths. The overall results of this report show that the majority of Canada’s wild species are ranked Secure. -
Data-Driven Identification of Potential Zika Virus Vectors Michelle V Evans1,2*, Tad a Dallas1,3, Barbara a Han4, Courtney C Murdock1,2,5,6,7,8, John M Drake1,2,8
RESEARCH ARTICLE Data-driven identification of potential Zika virus vectors Michelle V Evans1,2*, Tad A Dallas1,3, Barbara A Han4, Courtney C Murdock1,2,5,6,7,8, John M Drake1,2,8 1Odum School of Ecology, University of Georgia, Athens, United States; 2Center for the Ecology of Infectious Diseases, University of Georgia, Athens, United States; 3Department of Environmental Science and Policy, University of California-Davis, Davis, United States; 4Cary Institute of Ecosystem Studies, Millbrook, United States; 5Department of Infectious Disease, University of Georgia, Athens, United States; 6Center for Tropical Emerging Global Diseases, University of Georgia, Athens, United States; 7Center for Vaccines and Immunology, University of Georgia, Athens, United States; 8River Basin Center, University of Georgia, Athens, United States Abstract Zika is an emerging virus whose rapid spread is of great public health concern. Knowledge about transmission remains incomplete, especially concerning potential transmission in geographic areas in which it has not yet been introduced. To identify unknown vectors of Zika, we developed a data-driven model linking vector species and the Zika virus via vector-virus trait combinations that confer a propensity toward associations in an ecological network connecting flaviviruses and their mosquito vectors. Our model predicts that thirty-five species may be able to transmit the virus, seven of which are found in the continental United States, including Culex quinquefasciatus and Cx. pipiens. We suggest that empirical studies prioritize these species to confirm predictions of vector competence, enabling the correct identification of populations at risk for transmission within the United States. *For correspondence: mvevans@ DOI: 10.7554/eLife.22053.001 uga.edu Competing interests: The authors declare that no competing interests exist. -
A Mosquito Psorophora Ciliata (Fabricius) (Insecta: Diptera: Culicidae)1 Ephraim V
EENY-540 A Mosquito Psorophora ciliata (Fabricius) (Insecta: Diptera: Culicidae)1 Ephraim V. Ragasa and Phillip E. Kaufman2 Introduction For additional information on mosquitoes, see http://edis. ifas.ufl.edu/IN652. Psorophora ciliata (Fabricius) is a large mosquito (Cutwa and O’Meara 2005) that has developed an outsized reputa- tion because of its relatively intimidating heft and persistent Synonymy biting behavior (Gladney and Turner 1969), including Psorophora ciliata (Fabricius 1794) anecdotal historical accounts of its legendary aggressiveness Culex ciliata Fabricius (1794) (Wallis and Whitman 1971) and ‘frightening appearance’ Culex conterrens Walker (1856) (King et al. 1960). The ‘gallinipper’ or ‘shaggy-legged Culex molestus Weidemann (1820) gallinipper’ was used as a common name for Psorophora Culex rubidus Robineau-Desvoidy (1827) ciliata in various published reports (Ross 1947; King et al. Psorophora boscii Robineau-Desvoidy (1827) 1960; Breeland et al. 1961; Goddard et al. 2009). The term Psorophora ctites Dyar (1918) was mentioned much earlier by Flanery (1897) describing (From ITIS 2011) the mosquito as ‘the little zebra-legged thing—the shyest, slyest, meanest, and most venomous of them all’ [sic] but Distribution did not specify what species it was. The word gallinipper Psorophora ciliata usually is associated with other flood- originated as a vernacular term in the southeastern region water mosquitoes, including many species from the Aedes of the United States referring to ‘a large mosquito or other genera (Breeland et al. 1961), and has a wide distribution insect that has a painful bite or sting’ and has appeared in the New World. Floodwater mosquitoes often lay in folk tales, traditional minstrel songs, and a blues their eggs in low-lying areas with damp soil and grassy song referencing a large mosquito with a ‘fearsome bite’ overgrowth. -
The Mosquitoes of Minnesota
Technical Bulletin 228 April 1958 The Mosquitoes of Minnesota (Diptera : Culicidae : Culicinae) A. RALPH BARR University of Minnesota Agricultural Experiment Station ~2 Technirnl Rull!'lin :z2g 1-,he Mosquitoes of J\ilinnesota (Diptera: Culicidae: Culicinae) A. llALPII R\lm University of Minnesota Agricultural Experiment Station CONTENTS I. Introduction JI. Historical Ill. Biology of mosquitoes ................................ Zoogeography Oviposition ......................................... Breeding places of larvae ................................... I) Larrnl p;rowth ....................................... Ill ,\atural factors in the control of larvae .................. JI The pupal stage ............................................... 12 .\lating .................................... _ ..... 12 Feeding of adults ......................................... 12 Hibernation 11 Seasonal distribution II I\ . Techniques Equipment Eggs ............................... · .... · · · · · · · · · · · · · · · · · · · · · · · · · · · · · Larvae Pupae Adults Colonization and rearing . IB \. Systematic treatment Keys to genera Adult females . l'J \fale terminalia . 19 Pupae ······················································· .... ········ 2.'i Larvae ····················································· ..... ········ 2S :-n Anopheles ········································· ··························· Anopheles (Anopheles) barberi .................... · · · · · · · · · · · · · · · · · · · · · · · · earlei ...•......................... · · · · · -
Biology and Control of Aquatic Plants
BIOLOGY AND CONTROL OF AQUATIC PLANTS A Best Management Practices Handbook Lyn A. Gettys, William T. Haller and Marc Bellaud, editors Cover photograph courtesy of SePRO Corporation Biology and Control of Aquatic Plants: A Best Management Practices Handbook First published in the United States of America in 2009 by Aquatic Ecosystem Restoration Foundation, Marietta, Georgia ISBN 978-0-615-32646-7 All text and images used with permission and © AERF 2009 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means, electronic or mechanical, by photocopying, recording or otherwise, without prior permission in writing from the publisher. Printed in Gainesville, Florida, USA October 2009 Dear Reader: Thank you for your interest in aquatic plant management. The Aquatic Ecosystem Restoration Foundation (AERF) is pleased to bring you Biology and Control of Aquatic Plants: A Best Management Practices Handbook. The mission of the AERF, a not for profit foundation, is to support research and development which provides strategies and techniques for the environmentally and scientifically sound management, conservation and restoration of aquatic ecosystems. One of the ways the Foundation accomplishes the mission is by providing information to the public on the benefits of conserving aquatic ecosystems. The handbook has been one of the most successful ways of distributing information to the public regarding aquatic plant management. The first edition of this handbook became one of the most widely read and used references in the aquatic plant management community. This second edition has been specifically designed with the water resource manager, water management association, homeowners and customers and operators of aquatic plant management companies and districts in mind. -
Pesticide Discharge Management Plan
Pesticide Discharge Management Plan 1. PDMP Team a. Person(s) responsible for managing pests in relation to pest management area: All operational and biological support staff along with the Director of Mosquito Management Services (Wade Brennan, 5531 Pinkney Ave. Sarasota, FL. 34233) b. Person(s) responsible for developing and revising PDMP John Eaton, Operations Supervisor, and Wade Brennan Environmental Scientist III, are the individuals responsible for monitoring changes in Federal and State regulatory agencies that govern mosquito control operations. c. John Eaton, and Wade Brennan, are the individuals responsible for developing, revising and implementing corrective actions and other effluent requirements d. Person(s) responsible for pesticide applications Persons (supervisors and above) who direct applicators these include: All Operational staff employed by Sarasota County Mosquito Management Services that hold a Public Health Pest Control License administered by Florida Department of Agriculture and Consumer Services are directly responsible for pesticide applications (because they can oversee uncertified applicators) additionally, Sarasota County’s awarded Contractors must have required state certification (s). 2. Pest Management Area Description Overview Sarasota County Mosquito Management Services (SCMMS) has been mitigating pestiferous nuisance host seeking mosquitoes of public health importance for over 60 years. A total of forty- four mosquito species are found in Sarasota County of which a dozen are in need of management through a typical peak mosquito season, April through November. When intervention action plans are developed and implemented more than one species is usually involved. Past and current mitigation strategies for both larval and adult mosquitoes have always been in full compliance with FIFRA conditions which have met water quality standards. -
MOSQUITOES of the SOUTHEASTERN UNITED STATES
L f ^-l R A R > ^l^ ■'■mx^ • DEC2 2 59SO , A Handbook of tnV MOSQUITOES of the SOUTHEASTERN UNITED STATES W. V. King G. H. Bradley Carroll N. Smith and W. C. MeDuffle Agriculture Handbook No. 173 Agricultural Research Service UNITED STATES DEPARTMENT OF AGRICULTURE \ I PRECAUTIONS WITH INSECTICIDES All insecticides are potentially hazardous to fish or other aqpiatic organisms, wildlife, domestic ani- mals, and man. The dosages needed for mosquito control are generally lower than for most other insect control, but caution should be exercised in their application. Do not apply amounts in excess of the dosage recommended for each specific use. In applying even small amounts of oil-insecticide sprays to water, consider that wind and wave action may shift the film with consequent damage to aquatic life at another location. Heavy applications of insec- ticides to ground areas such as in pretreatment situa- tions, may cause harm to fish and wildlife in streams, ponds, and lakes during runoff due to heavy rains. Avoid contamination of pastures and livestock with insecticides in order to prevent residues in meat and milk. Operators should avoid repeated or prolonged contact of insecticides with the skin. Insecticide con- centrates may be particularly hazardous. Wash off any insecticide spilled on the skin using soap and water. If any is spilled on clothing, change imme- diately. Store insecticides in a safe place out of reach of children or animals. Dispose of empty insecticide containers. Always read and observe instructions and precautions given on the label of the product. UNITED STATES DEPARTMENT OF AGRICULTURE Agriculture Handbook No. -
ABSTRACT Title of Thesis: IMPROVING the SURVEILLANCE and CONTROL of VECTOR MOSQUITOES in HETEROGENEOUS LANDSCAPES Kaitlin
ABSTRACT Title of Thesis: IMPROVING THE SURVEILLANCE AND CONTROL OF VECTOR MOSQUITOES IN HETEROGENEOUS LANDSCAPES Kaitlin Michelle Saunders, Master of Science, 2020 Thesis Directed By: Paul T. Leisnham, Associate Professor, Department of Environmental Science and Technology Mosquitoes are often called the deadliest animals on earth, posing major public health issues in the United States and worldwide. The most common mosquito species in urban areas in the eastern United States are Aedes albopictus and Culex pipiens , which are vectors of numerous diseases including West Nile virus. Surveillance and management of Ae. albopictus and Cx. pipiens is particularly challenging due to the heterogeneity of urban landscapes, which change on relatively small spatial scales because of underlying social factors such as socioeconomic status (SES) and related infrastructure. As a result, mosquito habitat and distribution varies at correspondingly fine scales. The overall goal of my thesis is to assess relationships between SES and its associated environmental variables with Aedes and Culex mosquitoes in urban landscapes. The results of my research provide recommendations for integrated pest management strategies and highlight environmental justice issues related to disease transmission in low income areas. IMPROVING THE SURVEILLANCE AND CONTROL OF VECOR MOSQUITOES IN HETEROGENEOUS URBAN LANDSCAPES by Kaitlin Michelle Saunders Thesis submitted to the Faculty of the Graduate School of the University of Maryland, College Park, in partial fulfillment of the requirements for the degree of Master of Science 2020 Advisory Committee: Dr. Paul T. Leisnham, Chair Dr. Mitchell Pavao-Zuckerman Dr. Lance Yonkos © Copyright by Kaitlin Michelle Saunders 2020 Acknowledgements Many thanks to my thesis advisor, Dr. -
Mosquitoes and the Diseases They Transmit J
B-6119 6-02 Mosquitoes and the Diseases they Transmit J. A. Jackman and J. K. Olson* osquitoes are among the most important The length of time that a mosquito takes to complete insect pests affecting the health of people its life cycle varies according to food availability, weath- er conditions and the species of mosquito. Under favor- and animals. Biting female mosquitoes not M able conditions, some mosquitoes can complete their only irritate people and animals, but they can also entire life cycle in only 8 to 10 days. transmit many disease-causing organisms. Egg Annoying populations of mosquitoes can occur any- where in Texas because there are habitats favorable for One way to identify mosquito species almost everywhere in the state. the breeding sites of mosquitoes is to find the To control mosquitoes effectively, it helps to under- eggs. Mosquito eggs may stand their life cycle, to be able to identify the various be laid in clusters called kinds of mosquitoes, and to know what steps work best rafts on the water sur- for the different species and specific locations. face. They may also be laid singly on the water Life history surface or in dry areas Adult mosquito laying eggs. Mosquitoes have four distinct stages during their life that are flooded periodi- cycle: egg, larva, pupa and adult. The adult stage is free- cally. flying; the other stages are aquatic. When first laid, mosquito eggs are white, but within a few hours they become dark brown to black. The shape and size of mosquito eggs vary, with most being football- shaped or boat-shaped and 0.02 to 0.04 inch long. -
1980-1989 of NJMCA Annual Meetings
Table of Contents for 1980-1989 of NJMCA Annual Meetings Proceedings of the Association's annual meetings are published each year. The table of contents for Proceedings issued from 1980-1989 are available below. In addition to the referenced papers each proceedings includes a list of the NJMCA Officers, Trustees, Past Presidents, Mosquito Extermination Commissions and Mosquito Control Agencies in New Jersey and Business Meeting minutes. Papers of the 1989 NJMCA Annual Meeting • Presidential Address, Judy A. Hansen • Report from the State Mosquito Control Commission, Leonard E. Spiegel and Kenneth W. Bruder • An update on the Federal Endangered Species for Mosquito Control in Freshwater Wetlands, Teresa Yaegel-Souffront • Development of Best Management Practices for Mosquito Control in Freshwater Wetland, George O'Carroll and Kenneth W. Bruder • Worldwide Sata on Mosquitoes and Lyme Disease in 1988, Helen Scollers- Riedel • Introduction to the Symposium, Robert Kent -Moderator • The New Jersey Light Trap: An Old Standard for Most Mosquito Control Programs, William C. Reinert • The CDC Trap as a Special Monitoring Tool, James R. McNelly • Landing Rates and Bite Counts for Nuisance Evaluation, Roderic F. Schmidt • Complaints: An Underrated Surveillance Parameter, Michael Romanowski and Richard D. Huggins • Guidelines for Larval Surveillance, Claudia M. O'Malley • Closing Remarks, Robert Kent - Moderator • SYMPOSIUM: Lesser Known Mosquitoes in New Jersey • Introduction to the Symposium, James McNelly - Moderator • Psorophora howardii , a Species with an Increasing Range in New Jersey, Wayne J. Crans • Aedes dorsalis In New Jersey: Larval Habitat And Identification, Michael Romanowski • The Larval Habitat of Culex erraticus in Southern New Jersey, James R. McNelly and Wayne J.