University of South Florida Scholar Commons Graduate Theses and Dissertations Graduate School March 2019 DNA Barcoding of Fish Eggs in the Gulf of Mexico Makenzie Burrows University of South Florida,
[email protected] Follow this and additional works at: https://scholarcommons.usf.edu/etd Part of the Other Oceanography and Atmospheric Sciences and Meteorology Commons Scholar Commons Citation Burrows, Makenzie, "DNA Barcoding of Fish Eggs in the Gulf of Mexico" (2019). Graduate Theses and Dissertations. https://scholarcommons.usf.edu/etd/8342 This Thesis is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact
[email protected]. DNA Barcoding of Fish Eggs in the Gulf of Mexico by Makenzie Burrows A thesis submitted in partial fulfillment of the requirements for the degree of Master of Science College of Marine Science University of South Florida Major Professor: Mya Breitbart, Ph.D. Ernst Peebles, Ph.D. Steven A. Murawski, Ph.D. Date of Approval: March 7, 2019 Keywords: Genetics, molecular biology, PCR, spawning, Cuba, Florida Copyright © 2019, Makenzie Burrows ACKNOWLEDGEMENTS First, I would like to thank my advisor Dr. Mya Breitbart for giving me the opportunity to grow and succeed in her lab, and for her constant support, passion and guidance. I would also like to thank my committee members Dr. Ernst Peebles and Dr. Steve Murawski for their expertise in fish and their help with statistical analyses. Special thanks are due to Jeremy Browning, for picking out all the eggs for these projects, Eva-Maria Bonnelycke for helping me process all the Cuba samples, and Mason Kerr for helping me get through all of the processing complications.