Content

Antibodies for research in the following areas: Page Acrosomal Proteins 2 Cell Adhesion Molecules 3 Cell Cycle Associated Markers 15 Cell Signaling Research 17 Cluster Differentiation Markers 21 Collagens 70 Cytokines 75 Enzymes 83 Growth Factors and Receptors 86 Haematology 96 Hormones 99 Immunoglobulins 107 Keratins 110 Lymphoid Markers 116 Microbiology/Virology 124 Microtubuli 128 Multidrug Resistance Antigens 129 Muscles 135 Neuroscience 137 Special Interest 145 Tumour Markers 153 Secondary Reagents 170 Mouse Isotype Controls 171 Streptavidin 171 Aluminium Cryo-tubes for Storage in Liquid Nitrogen 172 Target Unmasking Fluid 172 General Technical Instructions for Use 173 Product Name Index 176 Product Code Index 189 Distributor Information 202

F = Frozen Sections (IHC) IP = Immunoprecipitation P = Paraffin Sections (IHC) IF = Immunofluorescence C = Flow Cytometry EM = Electron Microscopy E = ELISA A = Agglutination WB = Western Blotting B = Blocking IC = Immuno Cytology N = Neutralization Inh = Inhibition of biological activity FS = Functional Studies MC = Micro Cytotoxity Testing

All antibodies are reactive with human antigens, unless stated otherwise. Acrosomal Proteins Cell Adhesion Molecules

Intra-acrosomal protein, Bull Intra-acrosomal protein αvβ3 INTEGRIN β Catenin

Cat.no. MON 8015 (Monoclonal) Cat.no. MON 8017 (Monoclonal) Cat.no. MON 2032 (Monoclonal) Cat. no. MON 3055 (Monoclonal) NEW

Clone ACR.4 Clone Hs-14 Clone BV3 Clone 9F2 The antibody reacts with intra-acrosomal protein 28 kDa of bull The antibody reacts with human intra-acrosomal proteins (220 kDa). This monoclonal antibody (subclass IgG1) binds to human αvβ3 integrin Beta-catenin belongs together with alpha- and gamma-catenin to the spermatozoa, can be used for detection of state of acrosome of Strong reactivity was also observed with mouse sperms. Monoclonal present on human endothelium cells. catenin family. Catenins mediates cell-cell adhesion by interaction with undamaged spermatozoa. antibodies can be used (in IF test) for diagnostic of sperm pathology cadherins. Beta-catenin is highly homologous to gamma-catenin (quantitative detection of proteins) and for evaluation of physiological Use: IP, C (plakoglobin) although its function differs from that of plakoglobin. Use: IC, WB state of sperm cells (state of acrosome before or after acrosome Quantity: 100 µg Whereas plakoglobin has been found to suppress tumorigenicity, Quantity: 100 µg reaction). Finally, monoclonal antibodies could be useful for selection beta-catenin potentiates hyperproliferation and tumor formation. In of suitable method of Fertilization (IUI, standard IVF, ICSI) in the the nucleus beta-catenin it complexes with transcription factors and laboratories of assisted reproduction. αvβ3 INTEGRIN thus regulates the expression of specific genes. By its dual role, i.e. a Intra-acrosomal protein, Canine structural role in cell-cell junctions and a regulatory role in the nucleus, Use: IC, WB beta-catenin can transduce changes in cell adhesion and junction Quantity: 100 µg Cat.no. MON 2033 (Monoclonal) formation to control transmembrane signalling and gene expression. Cat.no. MON 8019 (Monoclonal) Clone BV4 Use: WB, IP, IF Clone Ds-2 Acrosin, Porcine This monoclonal antibody (subclass IgG1) binds to human αvβ3 integrin Quantity: 100 µg This antibody reacts with several forms of canine intra-acrosomal present on human endothelium cells. proteins (92 and 42 kDa). Antibody can be used for detection of the state of acrosome of undamaged spermatozoa. Cat.no. MON 9015 (Monoclonal) Use: WB, IP, C β1 INTEGRIN Quantity: 100 µg Use: IC, WB Clone ACR.2 Quantity: 100 µg The antibody reacts with various forms of porcine acrosin (55, 53, 45 Cat.no. MON 2031 (Monoclonal) and 35 kDa) in the acrosome of undamaged spermatozoa. Negative α Catenin reactivity was observed with human, bull and canine sperms. Clone BV7 Intra-acrosomal protein This monoclonal antibody (subclass IgG1) binds to human β1-integrin Use: IC, WB Cat. no. MON 3083 (Monoclonal) NEW chain present on tumour cells. BV7 is active on HT-29 colon carcinoma Quantity: 100 µg cells and on HCCP-2998 tumour cells. BV7 binds to several other tumour Cat.no. MON 8016 (Monoclonal) Clone 1G5 cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo The monoclonal antibody 1G5 reacts with human alpha-catenin. colon carcinoma) but does not affect adhesion to endothelial cells. Clone Hs-8 Alpha-catenin belongs together with beta- and gamma-catenin to the The antibody reacts with human and boar intra-acrosomal proteins catenin family. Catenins comprise a large family of Ca2+-dependent, Use: WB, IP, C, Inh. (220, 50, 34 and 21 kDa). Monoclonal antibodies can be used (in IF homotypic cell-cell adhesion molecules that play important roles in Quantity: 100 µg test) for diagnostic of sperm pathology (quantitative detection of development, epithelial cell polarity and tumor progression. Cadherins proteins) and for evaluation of physiological state of sperm cells (state are single pass, type I transmembrane proteins that are localized in of acrosome before or after acrosome reaction). Finally, monoclonal the adherens junction. To be fully functional, the cadherin cytoplasmic antibodies could be useful for selection of suitable method of domain must be linked to the actin cytoskeleton through a group of Fertilization (IUI, standard IVF, ICSI) in the laboratories of assisted proteins termed catenins: alpha-catenin, beta-catenin and plakoglobin. reproduction. Alpha catenin interacts with the cadherin indirectly via interactions with beta-catenin or plakoglobin. Use: IC, WB Quantity: 100 µg Use: WB, IP, IF Quantity: 100 µg

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Barmotin/7H6 antigen Desmoglein 2 E-Cadherin, uvomorulin, L-CAM E-Selectin, CD62E, Biotinylated

Cat. no. MON 3054 (Monoclonal) NEW Cat. no. MON 3057 (Monoclonal) NEW Cat.no. MON 7022 (Monoclonal) Cat.no. PS 017 (Polyclonal)

Clone 7H6 Clone 6D8 Clone 6F9 The antibody reacts with the Lectin Domain of E-selectin, CD62-E, Tight junctions function to maintain cellular polarity and permeability Desmoglein 2 is a member of the desmosomal cadherin family that One of the epithelial cell adhesion molecules, E-Cadherin, plays an formerly designated Endothelial Leucocyte Adhesion Molecule-1 barriers in epithelial and endothelial cells. Several molecules of the tight comprise the desmogleins and desmocollins. Desmosomes are cell-cell important role in the formation of cell-cell contacts in epithelia (ELAM-1). The antibody reacts with human endothelial cells activated junction complex have been identified. The monoclonal antibody 7H6 junctions in most epithelial cells and serve to link the intermediate irrespective their origin form ecto-, meso- or endodermal tissue. This with TNF, IL-1 or endotoxin. The antibody was found to react also with reacts with a 155 kD tight junction-associated protein also designated filament cytoskeletons of adjacent cells. Within the desmosome the early adhesion event between epithelial cells is a prerequisite for the cells transfected with the ELAM-1 gene. The antibody inhibits the barmotin. This protein is preferentially localized at the periphery of tight extracellular domain of desmoglein is essential for calcium dependent assembly of all elements of the junctional complex. Furthermore, adhesion of granulocytes both neutrophilic and eosinophilic. junctions of hepatocytes and other epithelia. The 7H6 antigen is also heterophilic binding to the desmocollins, whereas the intracellular E-Cadherin plays a crucial role in the maintenance of the epithelial detectable in the intestine and kidney. Studies with Madin-Darby canine domain is essential for binding to the desmosomal plaque protein, junctional complex and is as such an important molecule in maintaining Use: F, IP, WB kidney (MDCK) cells indicate the 7H6 antigen to be an important plakoglobin. Desmoglein 2 is synthesized in the basal layers, depending epithelial integrity. Over 90% of the malignant tumors are carcinomas. Quantity: 100 µg molecule for regulation of the barrier function of tight junctions. on the certain types of epidermis. One of the prerequisites for the release of carcinoma cells from the Confocal laser scanning microscopy revealed that the expression of The antibody 6D8 reacts with the 165 kDa desmoglein 2 protein. primary site might be a defect in intercellular adhesion mediated by 7H6 antigen decreased sequentially during hepatocarcinogenesis. Furthermore it is not cross reactive with desmoglein 1 or desmoglein 3. the absence of E-Cadherin expression. Therefore, the expression of E-selectin, CD62E, ELAM-1 The 7H6 antigen was expressed intensely at the apical and basolateral E-Cadherin might be an important parameter for the determination of membrane of cancer cells with glandular pattern. Use: WB, IP, IF the invasive potential of epithelial neoplasms, and for the transition of Examination of human gastric and colon cancer tissues showed that Quantity: 100 µg a benign to a malignant neoplasm. Antigen used: human uvomorulin. Cat.no. MON 6010 (Monoclonal) tight junctions were maintained highly in the well-differentiated Antigen location: lateral border of epithelial cells. (gland-forming) adenocarcinomas, but reduced in poorly-differentiated Clone ENA1 adenocarcinomas. In MDCK cells the disappearance of 7H6 expression Desmoglein 3 Use: WB, F, P ENA1 reacts with E-selectin CD62-E, previous designated as the is closely related with cell spreading in vitro induced hepatocyte growth Quantity: 1 ml Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1)[1-4]. The antibody factor/scatter factor. reacts with human endothelial cells activated with TNF, IL-1 or endotoxin. This antibody reacts with the human, canine, mouse, rat and chicken Cat. no. MON 3058 (Monoclonal) NEW The antibody was found to react also with cells transfected with the protein. E-Cadherin, uvomorulin, L-CAM ELAM-1 gene. The antibody inhibits the adhesion of granulocytes both Clone 5G11 neutrophilic and eosinophilic. Use: WB, F Desmoglein 3 is a member of the desmosomal cadherin family that Quantity: 100 µg comprise the desmogleins and desmocollins. Desmosomes are cell-cell Cat.no. MON 7024 (Monoclonal) Use: F junctions in most epithelial cells and serve to link the intermediate Quantity: 100 µg filament cytoskeletons of adjacent cells. Within the desmosome the Clone 5H9 Also available as BIOTIN conjugate (MON 6010B - 50 µg) Desmoglein 1 extracellular domain of desmoglein is essential for calcium dependent One of the epithelial cell adhesion molecules, E-Cadherin, plays an heterophilic binding to the desmocollins, whereas the intracellular important role in the formation of cell-cell contacts in epithelia domain is essential for binding to the desmosomal plaque protein, irrespective their origin form ecto-, meso- or endodermal tissue. This E-selectin, CD62E, ELAM-1, F(ab’)2 Cat. no. MON 3056 (Monoclonal) NEW plakoglobin. Desmoglein 3 is synthesized in both the basal and the lower early adhesion event between epithelial cells is a prerequisite for the suprabasal compartments. assembly of all elements of the junctional complex. Furthermore, Clone 27B2 The antibody 5G11 reacts with the 140 kDa desmoglein 3 protein. E-Cadherin plays a crucial role in the maintenance of the epithelial Cat.no. MON 6011 (Monoclonal) Desmoglein 1 is a member of the desmosomal cadherin family that Furthermore it is not cross reactive with desmoglein 1 or desmoglein 2. junctional complex and is as such an important molecule in maintaining comprise the desmogleins and desmocollins. Desmosomes are cell-cell epithelial integrity. Over 90% of the malignant tumors are carcinomas. Clone ENA2 junctions in most epithelial cells and serve to link the intermediate Use: WB, IP, IF, P One of the prerequisites for the release of carcinoma cells from the ENA2 reacts with E-selectin CD62-E, previous designated as the filament cytoskeletons of adjacent cells. Within the desmosome the Quantity: 100 µg primary site might be a defect in intercellular adhesion mediated by Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1)[1-4]. The antibody extracellular domain of desmoglein is essential for calcium dependent the absence of E-Cadherin expression. Therefore, the expression of reacts with human endothelial cells activated with TNF, IL-1 or endotoxin. heterophilic binding to the desmocollins, whereas the intracellular E-Cadherin might be an important parameter for the determination of The antibody was found to react also with cells transfected with the domain is essential for binding to the desmosomal plaque protein, the invasive potential of epithelial neoplasms, and for the transition of E-selectin gene. The antibody inhibits the adhesion of granulocytes both plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal a benign to a malignant neoplasm. Antigen used: Human uvomorulin. neutrophilic and eosinophilic. layers. Intact and functionally active desmoglein 1 is essential to Antigen location: lateral border of epithelial cells. epidermal integrity. Use: F The antibody 27B2 reacts with the 165 kDa desmoglein 1 protein. Use: WB, F, P Quantity: 1 ml Furthermore it is not cross reactive with desmoglein 2 or desmoglein 3. Quantity: 1 ml

Use: WB, IP, IF, F Quantity: 100 µg

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Ep-Cam (ESA) GP Ic, VLA-6 alpha-chain, CD49f ICAM-1, Intracellular Adhesion Molecule-1, ICAM-1, Intracellular Adhesion Molecule-1, CD54 CD54 Cat.no. MON 7021 (Monoclonal) Cat.no. MON 1128 (Monoclonal) Cat.no. MON 1016 (Monoclonal) Cat.no. MON 1133 (Monoclonal) Clone VU-1D9 Clone CLB-701 Ep-Cam (also called ESA, EGP40, 17-1A antigen, KSA, GA7333-2) is a The monoclonal antibody is directed against the CD49f antigen, Clone HM-1 Clone MEM-112 40 kD epithelial protein expressed on baso-lateral cell surfaces in very molecular weight 30/31, 120 kD (GP Ic or VLA-6 alpha-chain), which can The antibody reacts with the 90 kD glycoprotein Intracellular Adhesion This antibody reacts with human broadly expressed adhesive molecule many epithelial tissues (but absent from mesothelial tissues). The form distinct complexes with either the CD29 antigen (GP IIa or VLA Molecule-1 (ICAM-1). The adhesion molecule ICAM-1 belongs to the ICAM-1, the 90 kD glycoprotein Intracellular Adhesion Molecule-1 324AA have 3 potential glycosylation sites and is a trans membrane beta chain), resulting in the VLA-6 (alpha-6, beta-1) complex which is immunoglobulin super family, C2 subset; it is a ligand for the Integrins (CD54). The adhesion molecule ICAM-1 belongs to the immunoglobulin glycoprotein. The extracellular domain has a cysteine-rich repeat and a expressed on human platelets, or with the beta-4 integrin resulting in LFA-1 and MAC-1 and for CD43. family, it is a ligand for the integrin LFA-1. Immunogen originated from small domain with homology to nidogen. It is a homophyllic cell-cell the alpha-6, beta-4 complex which is expressed on various human Raji cell line. adhesion molecule, recently called Ep-Cam (Litvinov et al., 1994). It reacts epithelial cells. Platelet VLA-6 functions as a laminin receptor and platelet Use: F, IF with most epithelial cells and carcinomas. adhesion to laminin is inhibited by this antibody. The monoclonal Quantity: 100 µg Use: C, F, IF, WB antibody reacts with platelets, megakaryocytes, T-lymphocytes and Also available as BIOTIN conjugate (MON 1016B - 50 µg) Quantity: 100 µg Use: C, E, P, WB common acute lymphoblastic leukaemia cells (alpha-6, beta-1). In Quantity: 100 µg immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, ICAM-1, Intracellular Adhesion Molecule-1, ICAM-1, Intracellular Adhesion Molecule-1, placenta cyto- and syncytotrophoblasts. CD54 CD54, Biotinylated GPIIb/IIIa complex, CD41 Use: F, IF Quantity: 1 ml Cat.no. MON 1132 (Monoclonal) Cat.no. PS 018 (Polyclonal) Cat.no. MON 1120 (Monoclonal) Clone MEM-111 The antibody reacts with both intact human ICAM-1, CD54 and with Clone CLB-48 H-CAM, CD44, lymphocyte homing receptor This antibody reacts with human broadly expressed adhesive molecule soluble human ICAM-1. The antibody reacts also with ICAM-1 present Apparent molecular weight under reducing conditions: GPIIIa; 110 kD; ICAM-1, the 90 kD glycoprotein Intracellular Adhesion Molecule-1 on chimpanzee, rhesus monkey, cynomolgus monkey and baboon tissues. GPIIb alpha chain; 125 kD, beta chain; 25 kD. The monoclonal antibody (CD54). The adhesion molecule ICAM-1 belongs to the Immunoglobulin recognizes the intact platelet GPIIb/IIIa complex, i.e. it does not bind to Cat.no. MON 1150 (Monoclonal) family, it is a ligand for the intergrin LFA-1. Immunogen originated from Use: F, IP, WB dissociated GPIIb or GPIIIa. The antibody reacts with human trombocytes Raji cell line. Quantity: 50 µg and megakaryocytes. The antibody does not bind to platelets from Clone NKI-P2 patients with Glanzmann thrombasthenia and does not recognize the The antibody reacts with an 90 kD glycoprotein, the human antigen Use: F, IF, IP, WB, E vitronectin receptor, which contains GPIIa as a beta subunit (cf. CD61). CD44, also designated pgp-1. The CD44 antigen is widely expressed by Quantity: 100 µg Integrin α-6 chain, Laminin receptor, CDw49f The monoclonal antibody activates human thrombocytes and induces hematopoietic cells including T-lymphocytes, myeloid cells, macrophages, thrombocyte aggregation in the absence of trombocyte inhibitors such dendritic cells, red blood cells and brain, epithelial cell lines. The CD44 as EDTA. Immungen: human platelets. molecule is considered to be the lymphocyte homing receptor, H-CAM. ICAM-1, Intracellular Adhesion Molecule-1, Cat.no. MON 1176 (Monoclonal) The antibody blocks adhesion of lymphocytes to high endothelial venules. Use: IF CD54, conjugated Clone 4F10 Quantity: 1 ml Use: F, IF 4F10 is specific to the integrin α-6 chain which is expressed on epithelial Quantity: 1 ml Cat.no. MON 1132F / MON 1132R (Monoclonal) and endothelial basement membrane. This VLA α-6 chain, which consists of a single disulphide linked 120 kDa subunit and a small doublet Clone MEM-111 subunit of 25/30 kDa, is non covalently associated with the VLA β-chain This antibody reacts with the 90 kD glycoprotein Intracellular Adhesion in VLA-6 or / complex or with integrin chain ( / ). The H-CAM, CD44, lymphocyte homing receptor α6 β1 β4 α6 β4 Molecule-1 (ICAM-1). The adhesion molecule ICAM-1 belongs to the antigen is also expressed on platelets, monocytes, thymocytes and Integrin family, it is a ligand for the integrin LFA-1. Antigen specificity: T-lymphocytes. Cat.no. MON 1123 (Monoclonal) by flow cytometry, the main cellular reactivities are endothelial cells, activated. Use: F, IP Clone MEM-85 Quantity: 1 ml The antibody reacts with an 80 kD glycoprotein, the human antigen Use: C CD44, H-CAM. The CD44 antigen is present on T-lymphocytes, granu- Quantity: 100 µg locytes, brain, red blood cells and epithelial cell lines. The antibody reacts Available as FITC conjugate (MON 1132F - 100 µg) with the same epitope as Hermes-1 on the molecule, which is a leucocyte Available as R-PE conjugate (MON 1132R - 100 µg) adhesion molecule, (hyaluronan receptor), participating at homing.

Use: C, F, IF, IP Quantity: 100 µg Also available as FITC conjugate (MON 1123F - 100 µg) Also available as R-PE conjugate (MON 1123R - 100 µg)

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Junctional Adhesion Molecule-1 (JAM-1), Junctional Adhesion Molecule-1 (JAM-1) Junctional Adhesion Molecule-C (JAM-C), LFA-1 alpha chain, CD11a Mouse Mouse Cat. no. MON 3053 (Monoclonal) NEW Cat.no. MON 1151 (Monoclonal) Cat. no. MON 2069 (Monoclonal) NEW Cat. no. MON 4024 (Monoclonal) NEW Clone M.Ab.F11 Clone MEM-83 Clone BV12 Junctional adhesion molecule-1 (JAM-1) also known as the human Clone CRAM-18 F26 This antibody reacts with the α-chain of LFA-1, the CD11a/CD18 integrin Junctional adhesion molecule-1 (JAM-1) also known as the mouse platelet F11-Receptor (F11R) is a cell adhesion molecule (CAM). JAM-1 Junctional adhesion molecule-C (JAM-C) also known as JAM-2 is a 45 kD heterodimer present on human T- and B-lymphocytes, granulocytes and platelet F11-Receptor (F11R) is a cell adhesion molecule (CAM). JAM-1 is a member of the immunoglobulin superfamily found on the surface cell adhesion molecule (CAM). JAM-C is a transmembrane protein monocytes. The antibody activates the LFA-1 molecule leading to an is a member of the immunoglobulin superfamily found on the surface of human platelets and at intercellular junctions of endothelial cells which is a member of the immunoglobulin superfamily found at enhanced binding affinity for the ligand ICAM-1. Recognizes uniquely an of mouse platelets and at intercellular junctions of endothelial cells and epithelial cells. JAM-1 belongs together with JAM-2 and JAM-3 to intercellular junctions of endothelial cells. JAM-C belongs together with epitope involved in “activation” of LFA-1 (the mAb induces transition and epithelial cells. JAM-1 belongs together with JAM-2 and JAM-3 to a family of adhesion proteins with a V-C2 immunoglobulin domain JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of to high-affinity state). a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is adhesion proteins with a V-C2 immunoglobulin domain organization. organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It JAM plays an important role in tight junctions where it is involved in Use: IF, FS, IP involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cell-to-cell adhesion through homophilic interaction. It codistributes with Quantity: 100 µg codistributes with other tight junction components as ZO-1, 7H6 cingulin and occludin. JAM-1 plays a role in platelet aggregation, other tight junction components as ZO-1, 7H6 antigen, cingulin and antigen, cingulin and occludin. In human JAM-1 plays a role in platelet secretion, adhesion, spreading. occludin. JAM-C is potentially involved in the junctional sealing of the aggregation, secretion, adhesion and spreading. In the platelet F11R/Jam-1 is a membrane protein involved in 2 distinct vascular endothelium, in particular of high endothelial venules (HEV). LFA-3, CD58 processes initiated on the platelet surface. Antibody-induced platelet In adult murine tissue JAM-C expression is reported to be restricted to Use: C, E aggregation and secretion both dependent on FcgammaRII and GPIIb/ high endothelial venules of lymphoid organs, lymphoendothelial cells Quantity: 100 µg IIIa integrin, a process that may be involved in pathophysiological and endothelial cells in kidney. Monoclonal antibody CRAM-18 F26 also Cat.no. MON 1177 (Monoclonal) processes associated with certain thrombocytopenias. Antibody mediated reacts with human JAM-C. In humans, JAM-C expression is not restricted platelet adhesion independent from FcgammaRII or fibrinogen receptor to endothelial cells, but is also expressed on human lymphocytes. Clone BRIC 5 Junctional Adhesion Molecule-1 (JAM-1) and that appears to play a role in physiological processes associated LFA-3 is a membrane glycoprotein of 55-70 kDa which occurs either as with platelet adhesion and aggregation. A physiological role for the Use: IP, E, F, C a trans membrane moiety with a cytoplasmic domain or resident in the F11R/Jam-1 protein was demonstrated by its phosphorylation after the Quantity: 100 µg membrane. CD58 is a cell adhesion molecule of the Super Ig family, Cat. no. MON 3052 (Monoclonal) NEW stimulation of platelets by thrombin and collagen. A pathophysiological and facilitates antigen recognition via CD2. The antigen is present on role for the F11R/Jam-1 was revealed by demonstrating the presence erythrocytes, platelets, monocytes, bone marrow cells, a lymphocyte Clone BV16 of F11R/Jam-1 antibodies in patients with thrombocytopenia. LFA-1 alpha chain, CD11a subset and epithelial and endothelial cells. Junctional adhesion molecule-1 (JAM-1) also known as the human Adhesion of platelets through the F11R resulted in events characteristic platelet F11-Receptor (F11R) is a cell adhesion molecule (CAM). JAM-1 of the action of cell adhesion molecules (CAMs). Recent data suggests Use: C, WB, F is a member of the immunoglobulin superfamily found on the surface a role for F11R/Jam-1 in the adhesion of platelets to cytokine-inflamed Cat.no. MON 1104 (Monoclonal) Quantity: 1 ml of human platelets and at intercellular junctions of endothelial cells endothelial cells and thus in thrombosis and atherosclerosis induced in and epithelial cells. JAM-1 belongs together with JAM-2 and JAM-3 to non-denuded blood vessels by inflammatory processes. Clone MEM-25 a family of adhesion proteins with a V-C2 immunoglobulin domain The antibody is directed to the Lymphocyte Functional Antigen-1 (LFA-1), MAC1, C3bi receptor CR3, CD11b organization. JAM plays an important role in tight junctions where it is Use: C, WB, IP CD11a, which forms a heterodimer molecule (180-95 kD) consisting of involved in cell-to-cell adhesion through homophilic interaction. It Quantity: 100 µg the integrin molecules CD11a and CD18. The antigen plays a role in codistributes with other tight junction components as ZO-1, 7H6 antigen, intercellular communication between leukocytes and in adhesion of Clone BL-M/G 1 Cat.no. MON 1105A (Monoclonal) cingulin and occludin. JAM-1 plays a role in platelet aggregation, leukocytes to endothelial cells. The antibody blocks binding of LFA-1 to Clone BL-M/G 2 Cat.no. MON 1105B (Monoclonal) secretion, adhesion, spreading. ICAM-1. On endothelial cells the ligand of LFA-1 is ICAM-1 (CD54). Clone BL-M/G 4 Cat.no. MON 1105C (Monoclonal) In the platelet F11R/Jam-1 is a membrane protein involved in 2 distinct processes initiated on the platelet surface. Antibody-induced platelet Use: C, IP The antibodies react with the CR3 alpha chain of the CD11b/CD18 aggregation and secretion both dependent on FcgammaRII and GPIIb/ Quantity: 100 µg (p165, 95) integrin heterodimer present on human NK-cells, monocytes IIIa integrin, a process that may be involved in pathophysiological Also available as FITC conjugate (MON 1104F - 100 µg) and granulocytes. The antigens function as the C3Bi receptor. The processes associated with certain thrombocytopenias. Antibody mediated Also available as R-PE conjugate (MON 1104R - 100 µg) antibodies react strong with monocytes, weaker with granulocytes, also platelet adhesion independent from FcgammaRII or fibrinogen receptor a subpopulation of LGL/NK-cells is stained. and that appears to play a role in physiological processes associated with platelet adhesion and aggregation. A physiological role for the Use: C, F, IF F11R/Jam-1 protein was demonstrated by its phosphorylation after the Quantity: 1 ml stimulation of platelets by thrombin and collagen. A pathophysiological role for the F11R/Jam-1 was revealed by demonstrating the presence of F11R/Jam-1 antibodies in patients with thrombocytopenia. Adhesion of platelets through the F11R resulted in events characteristic of the action of cell adhesion molecules (CAMs). Recent data suggests a role for F11R/Jam-1 in the adhesion of platelets to cytokine-inflamed endothelial cells and thus in thrombosis and atherosclerosis induced in non-denuded blood vessels by inflammatory processes.

Use: C, F Quantity: 100 µg

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Nectin-2, Mouse Neural Cell Adhesion Molecule, NCAM, Neural Cell Adhesion Molecule, NCAM, P-selectin, GMP 140, PADGEM, CD62P, CD56 CD56 R-PE-conjugated Cat. no. MON 2092 (Monoclonal) NEW Cat.no. MON 9006 (Monoclonal) Cat.no. MON 9021 (Monoclonal) Cat.no. MON 1148R (Monoclonal) Clone 502-57 Nectin (originally isolated as poliovirus receptor-related protein (PRR)) Clone 123C3 Clone RNL-1 Clone CRC81 is a cell-cell adhesion molecule of the immunoglobulin supergene family. The antibody recognizes a trans membrane glycoprotein of 140 and The RNL-1 antibody, directed against N-CAM, is reactive with normal The monoclonal antibody is directed against the CD62-antigen, Nectin is colocalized with afadin at cadherin-based cell-cell adherence 180 kD which has been identified as NCAM (Neural Cell Adhesion neuronal tissues, and endocrine glands, such as pancreatic islets cells, (an integral membrane sialoglycoprotein 140 kD) also designated junctions in various tissues and cell lines. The nectin family consist of Module). It is involved in intercellular adhesion and play a role in pituitary gland, and adrenal medulla. Expression was also found in GMP-140 (granule membrane protein 140) or PADGEM, which is nectin-1, nectin-2 and nectin-3. Nectin-2 also known as PRR2 or CD112 outgrowth of neurites and the development of the nervous system. Leydig cells of the testis, in the thyroid and in smooth-muscle cells of expressed in platelet alpha granules that are translocated to the is a plasma membrane adhesion molecule localized at adherens junctions Several other isoforms have been identified which are expressed in a the small intestine, colon and bladder. This antibody is a valuable platelet surface upon activation with strong agonists. A simular protein, which is widely expressed in various cell lines including neuronal, developmental and tissue specific pattern. Two major epitopes have marker for the characterisation of several neuroendocrine tumours. present in endothelial cell Weibel-Palade bodies, is transiently expressed endothelial, epithelial and hematopoietic cells. Next to its role in been defined, NKI-nbl-1 (MON 9012) reacts with epitope 1 and 123C3 In immunoblotting the antibody is reactive with three main clusters on the surface of activated endothelial cells and functions as adhesion adherens junctions it functions as alphaherpus virus receptor and acts (MON 9006) reacts with epitope 2. At the international Workshop on of protein bands in respectively the 200 kDa, 100 kDa and 25-27 kDa molecule for granulocytes. The antibody does not interfere with the also as intercellular adhesion molecule and pseudorabies virus receptor. SCLC antibodies 123C3 has been categorized as cluster 1 antibody region. Antigen origin: small lung cancer cell line NC1-H82. Antigen functional activity of CD62 as an adhesion receptor for neutrophils. Disruption of mouse nectin-2 leads to infertility of male mice. Monoclonal (Stahel et al 1994). Most importantly, 123C3 can be used on routing location: cell surface. antibody 502-57 has been raised against the extracellular domain of formalin fixed paraffin section after retrieval. All cells in small cell Use: C mouse nectin-2. The antibody cross reacts with human nectin-2. carcinomas and carcinoids of the lung are strongly positive for 123C3. Use: F Quantity: 100 µg A minority of cases of other major types of lung carcinoma are Quantity: 1 ml Use: E, WB, IP, C, F sometimes positive as well: however this positivity is generally weak Quantity: 100 µg and focal. Adenoid cystic carcinomas of bronchial glands are strongly PECAM-1, CD31 positive. Neuroblastoma’s and Wilms tumors are usually also staining P-selectin, GMP-140, PADGEM, CD62P strongly positive. In non small lung cell carcinomas, 123C3 staining Nectin-3, Mouse has been associated with more advanced stage and a decreased survival Cat.no. MON 1199 (Monoclonal) after surgery (Kibbelaar et al. 1991). Positive staining with other tumors, Cat.no. MON 1137 (Monoclonal) include medullary thyroid carcinomas and some ovarian tumors. Clone BV8 Cat. no. MON 4000 (Monoclonal) NEW NCAM (CD56) is a marker for natural killer: 123C3 can be used to Clone CLB/thromb/6 This monoclonal antibody (subclaas IgG1) binds to human PECAM-1 distinguish NK-cells from other haematopoietic cell populations. Although The monoclonal antibody is directed against the CD62P-antigen, (platelet endothelial cell adhesion molecule-1; CD31) a specific of Clone 103-A1 expression of CD56 is uncommon among lymphomas, this feature has (an integral membrane sialoglycoprotein 140 kD) also designated endothelial cell junctions. It is also expressed in platelets and leukocytes. Nectin (originally isolated as poliovirus receptor-related protein (PRR)) defined a distinctive and important category of lymphoma: the putative GMP-140 or PADGEM, which is expressed in platelet alpha granules that is a cell-cell adhesion molecule of the immunoglobulin supergene family. natural killer (NK) cell lymphoma, which shows a predilection for the are translocated to the platelet surface upon activation with strong Use: E, IP, C Nectin is colocalized with afadin at cadherin-based cell-cell adherence upper aerodigestive tract, skin, skeletal muscle, and other extranodal agonists. A similar protein, present in endothelial cell Weibel-Palade Quantity: 100 µg junctions in various tissues and cell lines. The nectin/PRR-family, also sites and pursues an aggressive clinical course. Furthermore, this bodies, is transiently expressed on the surface of activated endothelial described as the alpha herpes virus receptor family consists of four antibody can be used to support diagnosis of lymphoma or to detect cells and functions as adhesion molecule for granulocytes. Immunogen: members, nectin-1, -2, -3 and -4. All the members of the nectin family residual disease for cases of CD56 positive T/NK-cell lymphoma in which Human Platelets. PECAM-1, CD31 have two or three slice variants. For nectin-3; nectin-3alpha, -3beta and the neoplastic lymphoid cells are small and show minimal atypia, -3gamma isoforms exist. Both nectin-2 and nectin-3 are ubiquitously especially in small biopsies. Use: IF expressed whereas nectin-1 is abundantly expressed in brain. Nectin-2 Quantity: 1 ml Cat.no. MON 1116 (Monoclonal) or -3 is expressed in cells where cadherin are not expressed, such as Use: P, F, IF blood cells and spermatids. Nectin-3 also known as PRR3 is a Quantity: 1 ml Clone CLB-HEC/75 transmembrane protein that is mainly expressed in testis and placental P-selectin, GMP 140, PADGEM, CD62P The monoclonal antibody is directed against the CD31 antigen, a 140 kD tissues. Nectin-3/PRR3 interacts in vivo with both long and short protein, which is expressed on human platelets. The antibody reacts isoforms of afadin. Monoclonal antibody 103-A1 has been raised against Neural Cell Adhesion Molecule, NCAM, with platelets, monocytes/macrophages, granulocytes and B-cells. It the recombinant mouse nectin-3 extracellular domain (50-400 aa). Cat.no. MON 1148 (Monoclonal) stains the cell boundaries of endothelial cells with a staining pattern Antibody 103-A1 does not react with human and rat nectin-3. CD56 identical to that of silver staining. Furthermore, in histology it stains Clone CRC81 weakly histocytes and glomeruli. Immunogen: human endothelial cells. Use: E, IP, C, F Cat.no. MON 9012 (Monoclonal) The monoclonal antibody is directed against the CD62-antigen, Quantity: 100 µg (an integral membrane sialoglycoprotein 140 kD) also designated, Use: F, IF Clone NKI-nbl-1 P-selectin, GMP-140 (granule membrane protein 140) or PADGEM, which Quantity: 100 µg The antibody recognizes a heterodimeric glycoprotein of 145, 185 kD is expressed in platelet alpha granules that are translocated to the which has been identified as NCAM (Neural Cell Adhesion Module). platelet surface upon activation with strong agonists. A similar protein, Two major epitopes have been defined, NKI-nbl-1 reacts with epitope 1 present in endothelial cell Weibel-Palade bodies, is transiently expressed and 123C3 (MON 9006) reacts with epitope 2. on the surface of activated endothelial cells and functions as adhesion molecule for granulocytes. The antibody does not interfere with the Use: F functional activity of CD62 as an adhesion receptor for neutrophils. Quantity: 1 ml (MON 9012-1) 5 ml (MON 9012-5) Use: IF, WB Quantity: 1 ml

10 For research use only. Not for drug, diagnostic or other use. www.monosan.com 11 Cell Adhesion Molecules Cell Adhesion Molecules

PECAM-1, CD31 Platelet GPIb, CD42b Platelets GPIIb/IIIa complex, Platelet GPIIIa, Vitronectin Receptor

alphaIIb/beta3-integrin, CD41a β-chain, CD61, β3-integrin Cat.no. MON 1142 (Monoclonal) Cat.no. MON 1146 (Monoclonal) Cat.no. MON 1144 (Monoclonal) Cat.no. MON 1147 (Monoclonal) Clone VM64 Clone VM16d The monoclonal antibody is directed against the CD31 antigen, a 140 kD The monoclonal antibody is directed against the CD42b antigen, platelet Clone VM16a Clone CRC54 protein, which is expressed on human platelets and endothelial cells glycoprotein GPIb, that serves as a receptor for von Willebrand factor The monoclonal antibody recognizes the intact platelet GPIIb/IIIa The anti CD61 antibody reacts with the platelet glycoprotein gpIIIa/

and is designated as PECAM-1 (platelet-endothelial cell adhesion and as a high affinity thrombin receptor. The antigen is expressed on complex (alphaIIb-/beta3), i.e. it does not bind to dissociated GPIIb or VNR β-chain. This glycoprotein is the common β-chain (β3 Integrin) of molecule). The antibody reacts with platelets, monocytes/macrophages, human platelets and megakaryocytes. The antigen is absent or present GPIIIa. The antibody reacts with human platelets and megakaryocytes. the cytoadhesins, gpIIbIIIa and VNR. The antibody reacts with platelets granulocytes and B-cells. It stains the cell boundaries of endothelial in very low levels on platelets of patients with the Bernard-Soulier Apparent molecular weight under reducing conditions: GPIIIa; 110 kD; and megakaryocytes. The antibody reacts preferentially with the cells with a staining pattern identical to that of silver staining. syndrome. VM16d inhibits low conc. thrombin (<0.1 U/ml) induced GPIIb alpha chain; 125 kD, beta chain; 25 kD. The antibody does not activated/occupied form of platelet GPIIb-IIIa (ligand-induced biding Furthermore in histology it stains weakly histocytes and glomeruli. platelet aggregation and decreases thrombin binding to platelets. bind to platelets from patients with Glanzmann thrombasthenia and site or LIBS epitope) and dissociated form of GPIIIa. CRC54-epitope is

F(ab’)2 and F(ab’) fragments are equally effective as whole intact does not recognize the vitronectin receptor, which contains GPIIIa as a located within the N-terminal region of GPIIIa (first 100 aminoacids). Use: F, IF, WB antibodies. The VM16d-epitope is located within the 45 kDa N-terminal beta subunit (cf. CD61). VM16a inhibits platelet Fc-receptor dependent CRC54 does not react with platelets of patients with Glanzmann Quantity: 1 ml domain of GPIb alpha-chain. VM16d does not effect platelet interaction reactions due to the proposed topographical association of the platelet thrombastenia. with von Willebrand factor, leading to the conclusion that the antibody Fc-receptor and the glycoprotein IIb-IIIa complex. reacts with or nearby the thrombin receptor site. Use: C, F, IF PECAM-1, CD31, Mouse Use: C, IF Quantity: 1 ml Use: IF Quantity: 1 ml Quantity: 1 ml Cat.no. MON 1149 (Monoclonal) VCAM-1, Vascular-Cell Adhesion Molecule-1, Platelet GPIIIa, Vitronectin Receptor CD106 Clone MEC7.46 Platelet GPIIb/ IIIa complex, The antibody reacts with the mouse form of the Platelet-Endothelial β-chain, CD61, β3-integrin Cell Adhesion Molecule. The reactivity of the antibody is restricted to alphaIIb/beta3-integrin, CD41a Cat.no. MON 6017 (Monoclonal) the isoform of the molecule that is selectively expressed by endothelial Cat.no. MON 1051 (Monoclonal) cells. The antibody precipitates a 130 kDa molecule present on the Cat.no. MON 1145 (Monoclonal) Clone 1G11B1 membrane of endothelial cell presents on all mouse blood vessels both Clone BL-E6 The antibody reacts with VCAM-1, the vascular cell adhesion molecule in normal and inflamed or tumour tissues. The antigen is predominantly Clone CRC64 The anti CD61 antibody recognizes the platelet glycoprotein gpIIIa/ (1), which is a member of the immunoglobulin super family. The antigen

present at the lateral borders of endothelial cells as described for The monoclonal antibody recognizes the intact platelet GPIIb/IIIa VNR β-chain. This glycoprotein is the common β-chain (β3 Integrin) of is induced on human endothelial cells by TNF, IL-1, IFN-γ or endotoxin. human PECAM-1. complex (alphaIIb-/beta3-integrin), i.e. it does not bind to dissociated the cytoadhesins, gpIIIbIIIa and VNR. The antibody reacts with thrombo- The ligand for VCAM-1 is VLA-4 (CD49d/CD29) an integrin that is GPIIb or GPIIIa. The antibody reacts with human platelets and mega- cytes and megakaryocytes. constitutively expressed by thymocytes, lymphocytes and monocytes. Use: IP, C karyocytes. Apparent molecular weight under reducing conditions: Quantity: 100 µg GPIIIa; 110 kD; GPIIb alpha chain; 125 kD, beta chain; 25 kD. The Use: F Use: F, E Also available as BIOTIN conjugate (MON 1149B - 50 µg) antibody does not bind to platelets from patients with Glanzmann Quantity: 1 ml Quantity: 100 µg thrombasthenia and does not recognize the vitronectin receptor, Also available as BIOTIN conjugate (MON 6017B - 50 µg) which contains GPIIIa as a beta subunit (cf. CD61). CRC64 (both intact antibody and F(ab’) fragments) inhibits platelet aggregation by Plakoglobin (gamma-catenin) 2 Platelet GPIIIa, Vitronectin Receptor blocking fibrinogen binding to the glycoprotein IIb-IIIa complex. VLA-α3 chain, Collagen and Fibronectin β-chain, CD61, β3-integrin Conjugated Cat. no. MON 3059 (Monoclonal) NEW Use: C, IF receptor, CD49c Quantity: 1 ml Cat.no. MON 7051B/F (Monoclonal) Clone 15F11 Cat.no. MON 1274 (Monoclonal) Plakoglobin, also known as gamma-catenin belongs together with Clone CLB-thromb/1 alpha- and beta catenin to the catenin family. Catenins mediates cell-cell This antibody recognizes the CD61 antigen. Also known as integrin , Clone MIKd2 Platelet GPIIb/IIIa complex, β3 adhesion by interaction with cadherins. Plakoglobin is found in the CD61 antigen is expressed on platelets, megakaryocytes, osteoclasts The antibody recognizes the VLA-α3 chain of the VLA-3 complex and desmosomes and adherens junctions. Plakoglobin is highly homologous alphaIIb/beta3-integrin, CD41a and endothelial cells. is reactive with epithelial tissue and basal renal distal tubules. Reacts to beta-catenin although its function differs from that of beta-catenin. with human, other species not tested. Whereas beta-catenin has been found in potentiating hyperproliferation Cat.no. MON 1173 (Monoclonal) Use: C and tumor formation, plakoglobin can suppress tumorigenicity. Quantity: 0.5 ml Use: C, IP Overexpression of plakoglobin has been shown to suppress cell Clone M148 Available as BIOTIN conjugate (MON 7051B - 0.5 ml) Quantity: 1 ml proliferation and cell tumorigenicity in animals. Furthermore reduced The antibody is specific to glycoprotein IIb/IIIa complex on platelets, Available as FITC conjugate (MON 7051F - 0.5 ml) plakoglobin expression has been found in tumor tissues and metastatic megakaryocytes, and monocytes. Acute megakaryoblastic leukaemia lesions of renal cells, esophageal carcinomas and in skin carcinomas. cells are positive for the gpIIb/IIIa antigen whereas common acute Antibody 15F11 cross reacts with rat and weakly with mouse. lymphoblastic leukaemia and acute myeloid leukaemia are negative.

Use: WB, IP, IF Use: IF Quantity: 100 µg Quantity: 1 ml

12 For research use only. Not for drug, diagnostic or other use. www.monosan.com 13 Cell Adhesion Molecules Cell Cycle Associated Markers

VLA-α4 chain, CDw49d Citrate synthetase Golgi zone

Cat.no. MON 1175 (Monoclonal) Cat.no. MON 2057 (Monoclonal) Cat.no. MON 8055 (Monoclonal)

Clone 44H6 Clone 8D3-G4 Clone AE-6 The antibody reacts with the 150 kDa VLA-α4 chain (lymphocyte homing 8D3-G4 specifically recognize the enzyme citrate synthetase. This enzyme AE-6 recognizes an antigen found in the golgi zone of human cells. receptor) which is associated with the CD29 molecule in the VLA-4 is involved in the oxidative metabolism (Krebs’ cycle) and abundantly Source: A BALB/c mouse was immunized with Su-DHL-1 large lymphoma complex. Expressed ALL, AML, T- and B-cells, monocytes, thymocytes present in mitochondrial compartment of the cell. cells. The isolated splenocytes were fused with mouse myeloma cells. and Langerhans cells. No reactivity with human kidney or brain sections. Immunogen: Hoon pre-B leukaemia cell line. Use: F Use: F, P, C Quantity: 100 µg Quantity: 100 µg Use: C, IP Quantity: 1 ml DAXX Histone H1

Cat.no. MON 5000 (Monoclonal) Cat.no. MON 3048 (Monoclonal)

Clone DAXX-01 Clone AE-4 Daxx is a novel regulator/modulator of apoptosis implicated in Fas – and AE-4 recognizes histone H1 in the nuclei of animal and plant cells. TGF-b-induced apoptotic pathways. It was also found to be associated It produces a speckled nuclear staining pattern in normal and malignant with PML protein in discrete nuclear structures known as PML oncogenic cells. Tonsil is the recommended positive control tissue. Source: domains (PODs). Daxx as predominantly nuclear protein also functions A BALB/c mouse was immunized with human leukaemia biopsy cells. as a repressor of Pax3 and Ets1 induced transactivation. Interestingly, The isolated splenocytes were fused with mouse myeloma cells. in association with ASK1 kinase Daxx translocates from nuclei to cytoplasm. Though Daxx is thought to be mainly pro-apoptotic protein, Use: F, P, E, WB extensive embryonic apoptosis in Daxx-deficient mice as well as its Quantity: 100 µg anti-apoptotic role in CD43-induced apoptosis suggest a promiscuous function of Daxx in the regulation of apoptosis. Nuclear membrane Use: WB, IC Quantity: 100 µg Cat.no. MON 7061 (Monoclonal)

Double stranded DNA Clone AE-5 AE-5 recognizes an antigen associated with the nuclear membrane expressed in human cells. Source: A BALB/c mouse was immunized with Cat.no. MON 6030 (Monoclonal) nuclei of myeloid leukaemia biopsy cells. The isolated splenocytes were fused with mouse myeloma cells. Clone AE-2 AE-2 recognizes double stranded DNA expressed in the nucleus of Use: F, P, C human cells. Source: A BALB/c mouse was immunized with nuclei of Quantity: 100 µg Raji Burkitt’s lymphoma cells. The isolated splenocytes were fused with mouse myeloma cells. NuMa Use: F, P, C Quantity: 100 µg Cat.no. MON 6029 (Monoclonal)

Clone A73-B/D12 A73-B/D12 recognizes a cell nucleus and mitotic cell antigen and specifically poles of the mitotic spindle, adjacent microtubules and centrosomes. Source: A NZB mouse was immunized with live Ls 174T cells (colon carcinoma). Splenocytes were fused with mouse myeloma X63-Ag8.653 cells.

Use: F, E, WB Quantity: 100 µg

14 For research use only. Not for drug, diagnostic or other use. www.monosan.com 15 Cell Cycle Associated Markers Cell Signaling Research

P34cdc-2 Nuclear Protein Ribonucleoprotein Particles BLNK (B cell linker protein), Mouse Csk-binding protein (PAG)

Cat.no. MON 7039 (Monoclonal) Cat.no. MON 8034 (Monoclonal) Cat. no. PS280 (Polyclonal) NEW Cat. no. MON 2081 (Monoclonal) NEW

Clone POH-1 Clone 58-15 BLNK (B cell linker protein) is an adaptor molecule that plays key role Clone PAG-C1 POH-1 detects the three bands within the 34 kD region corresponding This antibody identifies cells active in the cell cycle and hence can be in B cell activation initiated by cross-linking the B cell receptor (BCR). The antibody recognizes an epitope located in the C-terminal domain to the p34 protein and its cleavage products. It is positive in immuno- used to measure the mitotic activity of cell populations. Since the This activation leads to an increase in the tyrosine phosphorylation of of Csk-binding protein (Cbp), also known as protein associated with blotting on HeLa cell lysate and negative on fibroblast (LEP strain) cell antibody can be used in paraffin embedded tissue sections, it can identify a number of proteins due to activation of nonreceptor protein tyrosine glycosphingolipid-enriched microdomains (PAG). lysate. Source: A BALB/c mouse was immunized with recombinant human actively cycling cells within routinely fixed tissue specimens. Source: kinases (PTK) including Syk, which is responsible for the tyrosine Immunogen: C-terminal peptide (last 15 amino acids) of human Csk p34.cdc2 fusion protein. Splenocytes were fused with mouse myeloma A BALB/c mouse was immunized with isolated nuclei. Splenocytes were phosphorylation of BLNK. BLNK is cytosolic protein identified as two binding protein coupled to KLH. P3-X63-Ag8.653 cells. fused with mouse myeloma NS-1 cells. phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively Cross reactive with Human, Mouse, Rat, Bovine, Other not tested. spliced forms of human BLNK). Mapping of the tyrosine residues in Use: P, WB Use: F, P, C BLNK reveal three tyrosines that bind PLC-gamma2, and two tyrosines Use: IP Quantity: 100 µg Quantity: 100 µg that bind the Tec family kinase Btk, the Vav nucleotide exchange factor Quantity: 100 µg and the adaptor Nck.

P105 (PANA) WAF1/p21 protein, Human Use: WB Csk-binding protein (PAG) Quantity: 100 µg

Cat.no. MON 7038 (Monoclonal) Cat.no. MON 7048 (Monoclonal) Cat. no. PS279 (Polyclonal) NEW Csk-binding protein (Cbp/PAG) Clone 2B3 Clone WA-1 Csk binding protein, also known as PAG (phosphoprotein associated Protein p105, found in all human cells, is a proliferation-associated The mAb WA-1 reacts with human and other mammalian p21 protein. with GEMs), is a ubiquitously expressed 46 kDa transmembrane nuclear antigen which is absent in non-cycling cells in Go. It is a protein The intracellular protein p21 is a 21 kD protein and also known as Cat. no. MON 4022 (Monoclonal) NEW adaptor protein present in membrane rafts (glycosphingolipid-enriched component of nuclear spliceosomes (Nature 343, 437-441, 1990), wild-type p53-activated fragment 1 (WAF1). It is an inhibitor of cyclin- microdomains), which however migrates on SDS PAGE gels anomalously a site of RNA splicing. During G2 and mitosis, p105 is dramatically dependent kinases (Cdks) and proliferating-cell nuclear antigen (PCNA). Clone MEM-255 as an 80 kDa molecule. Following tyrosine phosphorylation by Src family increased, possibly due to the unmasking of nucleolar antigen resulting Human protein associated with glycosphingolipid-enriched microdomains kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, in a large accumulation of antigen in the mitotic cytoplasm. On Para Use: F, P, E, WB (PAG)/Csk-binding protein (Cbp). the major negative regulator of the Src family kinases. Signaling via formaldehyde fixed human cells, 2B3 produces a speckled nuclear Quantity: 100 µg the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) staining pattern in cycling cells. Cells in mitosis show a very bright Use: WB in mast cells leads to PAG increased tyrosine phosphorylation and Csk homogeneous pattern in the mitotic cytoplasm. Chromosomes show a Quantity: 100 µg binding, while T cell receptor signaling causes PAG dephosphorylation, halo pattern of staining and the mitotic spindle appears unstained. loss of Csk binding and increased activation of the protein tyrosine Immunogold electron microscopy demonstrates specific staining of kinase Lck. nuclear interchromatin granules (spliceosomes). Csk Reactive with human and mouse. Use: F, P, WB Use: WB, IP Quantity: 100 µg Cat. no. MON 3088 (Monoclonal) NEW Quantity: 100 µg

Clone CSK-04 Proliferating Cell Nuclear Antigen (PCNA) C-terminal Src kinase (Csk) is a non-receptor protein tyrosine kinase. LAT One of its major functions is to specifically phosphorylate a conserved C-terminal tyrosine of Src family kinases thereby down-regulating Cat.no. MON 6004 (Monoclonal) their kinase activity. CSK is expressed ubiquitously as a protein of Cat.no. MON 4023 (Monoclonal) approximately 50 KDa in cytosol, but a fraction distributes to lipid rafts Clone PC10 due to interaction with the lipid raft-associated protein PAG Clone LAT-01 Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin, is a (Csk-binding protein). The Linker for Activation T-cells (LAT) is the trans membrane adapter 36 kD nuclear protein, involved in the DNA replication process. The Reactive with human and mouse. protein (36-38kDa) which plays an important role in the transduction of PCNA reaches its maximum expression level during the S-phase of the TCR-initiated, T-cell specific signaling events. Mab LAT-01 is suitable for cell cycle. The presence of PCNA in the cell cycle correlates with the Use: IP, WB detection of LAT by Western Blotting as well as for immunoprecipitation expression of the Ki-67 antigen and with the incorporation of BrdU. Quantity: 100 µg studies. LAT-01 does not cross reacts with mouse homologues. PCNA is and excellent marker of proliferative cells in routinely processed tissue sections. This antibody cross reacts with all vertebrates. Antigen Use: WB, IP localisation: nuclear. Immunogen: Rat PCNA made in the protein A Quantity: 100 µg expression vector pR1T2T.

Use: WB, P Quantity: 1 ml

16 www.monosan.com For research use only. Not for drug, diagnostic or other use. 17 Cell Signaling Research Cell Signaling Research

Lck-interacting molecule (LIME) Lck-interacting molecule (LIME), Mouse SHP2 interacting TRAP (SIT) SOCS3

Cat. no. MON 3079 (Monoclonal) NEW Cat. no. PS277 (Polyclonal) NEW Cat.no. MON 4021 (Monoclonal) Cat. no. MON 3090 (Monoclonal) NEW

Clone LIME-06 The antibody reacts specifically with mouse LIME Clone SIT-01 Clone SO4 LIME (Lck-interacting molecule) is a new raft-associated adaptor protein (Lck-interacting molecule). The antibody reacts with human SHP2-interacting trans membrane The cytokine signaling is known to be regulated by a negative feedback involved in regulation of T cell activation by coreceptors. LIME becomes Immunogen: Bacterially expressed recombinant fragment of mouse LIME. adapter protein (SIT). Weaker reactivity was observed with mouse regulator called suppressor of cytokine signaling (SOCS) inhibitors tyrosine phosphorylated after cross-linking of the CD4 or CD8 homolog. family. Members of the SOCS family have been identified due to their coreceptors. Phospho-LIME associates with the Src family kinase Lck Use: WB, IP marked homology on the SH2 domain and a unique conserved motif and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T Quantity: 100 µg Use: WB, IP referred to as the SOCS box. Expression of SOCS3 is transiently induced cells results in an increase of Csk in lipid rafts, increased phosphorylation Quantity: 100 µg in a variety of immune and inflammatory situations by a wide variety of Lck and higher Ca2+ response to CD3 stimulation. of cytokines. SOCS3 is also involved in growth hormone function, NTAL leptin and insulin signaling. SOCS3 inhibits Janus kinase (JAK) tyrosine Use: IP SLP-76 kinase activity. Quantity: 100 µg Cat. no. MON 2082 (Monoclonal) NEW Use: WB, IC Cat. no. MON 3085 (Monoclonal) NEW Quantity: 100 µg Lck-interacting molecule (LIME) Clone NAP-03 The antibody recognizes the defined epitope (aa 196-212) of Non-T Clone SLP-76/03 cell activation linker (NTAL), also known as LAB (linker of activated B SLP-76 (SH2 domain-containing leukocyte protein) is an adaptor STAT 1 Cat. no. MON 3082 (Monoclonal) NEW cells), a 30 kDa transmembrane adaptor protein present in membrane molecule that is involved in T cell development and activation. microdomains (rafts) of B cells, NK cells and myeloid cells. Thymocytes from transgenic mice expressing a SLP-76 mutant with Clone LIME-10 deletion of the amino terminus are unable to develop to CD4-CD8- stage. Cat. no. MON 2089 (Monoclonal) NEW LIME (Lck-interacting molecule) is a new raft-associated adaptor protein Use: WB, IP Mature T cells do develop in mice expressing SLP-76 with mutations in involved in regulation of T cell activation by coreceptors. LIME becomes Quantity: 100 µg proline rich region or SH2 domain, but they fail to upregulate CD69 or Clone SM1 tyrosine phosphorylated after cross-linking of the CD4 or CD8 proliferate appropriately in response to anti-CD3 stimulation. SLP-76 is The antibody recognizes an epitope included within amino acids 721- coreceptors. Phospho-LIME associates with the Src family kinase Lck cytosolic protein identified as phosphoprotein migrating at 76 kDa in 733 of the 91 kDa STAT 1 protein. Cross reactive with Human, Mouse, and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T NTAL SDS-PAGE. Amino-terminal domain of SLP-76 containing several tyrosines Other not tested. cells results in an increase of Csk in lipid rafts, increased phosphorylation is followed by PR (proline rich) region and an SH2 domain. SLP-76 of Lck and higher Ca2+ response to CD3 stimulation. associates with LAT via Gads or Grb 2. The PR region of SLP-76 Use: IP, WB Cat. no. MON 2083 (Monoclonal) NEW constitutively associates with carboxy-terminal SH3 domain of Gads Quantity: 100 µg Use: WB and P-1 segment of the PR region was also identified that associates Quantity: 100 µg Clone NAP-07 with the SH3 domain of PLC-gamma1. The PR region therefore provides The antibody reacts with Non-T cell activation linker (NTAL), also known another pathway for PLC-gamma1 recruitment to the LAT signaling SYK as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor complex in T cells. The SH2 domain of SLP-76 has been identified as Lck-interacting molecule (LIME) protein present in membrane microdomains (rafts) of B cells, NK cells the region involved in binding the serine/threonine kinase HPK1. HPK1 and myeloid cells. may act as both a positive and a negative regulator by promoting the Cat. no. MON 2084 (Monoclonal) NEW Jnk mitogen activated protein kinase (MAPK) pathway and inhibiting Cat. no. PS276 (Polyclonal) NEW Use: WB, IP the pathway leading to AP-1 activation. Clone SYK-01 Quantity: 100 µg Reactive with human, mouse and porcine. The antibody reacts with Protein tyrosine kinase p72Syk (Syk family LIME (Lck-interacting molecule) is a new raft-associated adaptor protein tyrosine-specific phosphotransferase. Cross reactive with Human, Mouse, involved in regulation of T cell activation by coreceptors. LIME becomes Use: WB Rat, Other not tested. tyrosine phosphorylated after cross-linking of the CD4 or CD8 PAG/Cbp Quantity: 100 µg coreceptors. Phospho-LIME associates with the Src family kinase Lck Use: IP, WB, IC and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T Quantity: 100 µg cells results in an increase of Csk in lipid rafts, increased phosphorylation Cat.no. MON 4022 (Monoclonal) SLP-76 of Lck and higher Ca2+ response to CD3 stimulation. Clone MEM-255 T-cell receptor interacting molecule (TRIM) Use: WB The antibody reacts with human protein associated with glycosphin- Cat. no. PS278 (Polyclonal) NEW Quantity: 100 µg golipid-enriched micro domains (PAG)/Csk-binding protein (Cbp). The antibody reacts with SLP-76 (SH2 domain-containing leukocyte Cat.no. MON 4020 (Monoclonal) Use: WB protein), 76kDa cytosolic adaptor molecule that is involved in T cell Quantity: 100 µg development and activation. Clone TRIM-04 This antibody recognizes the human T-cell receptor interacting Use: WB molecule (TRIM) Quantity: 100 µg Use: WB, IP Quantity: 100 µg

18 For research use only. Not for drug, diagnostic or other use. www.monosan.com 19 Cell Signaling Research Cluster Differentiation Markers

TCR (V beta 5 related) CD1 CD1b

Cat. no. MON 2090 (Monoclonal) NEW Cat.no. MON 1022 (Monoclonal) Cat.no. MON 2039 (Monoclonal)

Clone MEM-262 Clone RIV12 Clone 100-1A5 The antibody recognizes beta chains of the TCR expressed by HPB-ALL RIV12, selected by ELISA, cytotoxicity tests and FACS analysis appeared 100-1A5 binds with CD1b which is a 45 kD membrane glycoprotein cell line (carrying V(beta5.3) and a small subset of peripheral blood T to be an CD1 reacting with a 49 kDa molecule on human thymocytes. expressed together with beta2 microglobulin. A BALB/c mouse was cells. This subset is larger than that recognized by other V(beta5.3)- Source: A BALB/c mouse was immunized with human peripheral immunized with stimulated human leucocytes. Splenocytes were fused specific antibodies. Cross reactive with Human, Other not tested. lymphocytes. Splenocytes of the mouse were fused with mouse myeloma with mouse myeloma NS1-cells. cells of the 653 cell line. Use: C, IP, WB, FS Use: F, C Quantity: 100 µg Use: F, C, E Quantity: 100 µg Quantity: 100 µg

CD2, T-cells CD1a Cat.no. MON 1066 (Monoclonal) Cat.no. MON 1061 (Monoclonal) Clone BL-TP 2/1 Clone BL-THY 1 The antibody reacts with a 50 kD molecule specific for the CD2 antigen. The antibody reacts with a 49 kD molecule, associated with This antigen is also known as sheep erythrocyte receptor and functions β-2-microglobulin, specific for the CD1a antigen. The antibody reacts as receptor for the CD58-antigen (LFA-3). The monoclonal antibody reacts with 70-80% of the human thymocytes (mainly corticoid) as well as with all human peripheral T-lymphocytes and a fraction of the NK-cell with cells from some leukaemia’s and lymphomas of T-cell origin. In (LGL) population. In histology the antibody stains T-lymphocytes. tissues sections the antibody reacts also with Langerhans cells. Use: C, F, IF Use: C, F Quantity: 1 ml Quantity: 1 ml

CD3 CD1a Cat.no. MON 1062 (Monoclonal) Cat.no. MON 1023 (Monoclonal) Clone BL-TP 3b Clone CBT6 The antibody reacts with a 20 kD molecule specific for the CD3 antigen. CBT6 specifically reacts with CD1a, a trans membrane glycoprotein of 49 kD which is non-covalently associated with beta-microglobulin. The Use: C, F, IF antigen is strongly expressed on cortical thymocytes and dendritic cells Quantity: 1 ml and disappear at later stages of maturation. Source: A BALB/c mouse was immunized with Jurkat T-ALL cells. After three weeks the mouse was boosted and the splenocytes fused with myeloma cell line NS0. CD3 A positive hybridoma obtained after three limiting dilutions served as source for the hybridoma. Cat.no. MON 1004 (Monoclonal) Use: F, E Quantity: 100 µg Clone MEM-57 Human CD3 T-Cell antigen; 20 kDa recognizes an epitope on CD3 and δ-chain. Can be used for immunoisolation of the antigen and for immunostaining of T-cells.

Use: IP, C, FS Quantity: 100 µg

20 www.monosan.com For research use only. Not for drug, diagnostic or other use. 21 Cluster Differentiation Markers Cluster Differentiation Markers

CD3, conjugated CD3 CD4, Helper T-cell CD5, pan T-cell

Cat.no. MON 1004F/R (Monoclonal) NEW Cat.no. MON 1003 (Monoclonal) Cat.no. MON 2048 (Monoclonal) Cat.no. MON 1063 (Monoclonal)

Clone MEM-57 Clone SPV-T3b Clone MEM-241 Clone BL-TP 5a(6E3) Human CD3 T-Cell antigen; 20 kDa recognizes an epitope on CD3 and This antibody reacts with CD3, 19-29 kD, present on all resting and Human CD4 antigen is a trans membrane glycoprotein (59 kDa) of the The antibody reacts with a 67 kD molecule specific for the CD5 antigen. δ-chain. Can be used for immunoisolation of the antigen and for activated human T-cells, on T-leukaemia cells and a proportion of human immunoglobulin supergene family present on subset of T-lymphocytes The antigen is present on the majority of circulating and non-circulating immunostaining of T-cells. thymocytes. Immunogen: HG-38 (“helper/inducer” T-cells) and also expressed at a lower level on T-cells as well as on the majority of cortical and medullary thymocytes. monocytes and granulocytes. MAb MEM-241 exhibits superior perfor- The antigen is also present on a subpopulation of B-lymphocytes. In Use: C Use: F, IF mances in cytofluorometric analysis of cells and is excellent for the histology the antibody stains in lymphoid tissues primarily mature Quantity: 100 tests Quantity: 1 ml (MON 1003-1) detection of CD4 by Western blotting (under non-reducing conditions). T-lymphocytes and a small subpopulation of B-lymphocytes. Available as FITC conjugate (MON 1004F - 100 tests) 5 ml (MON 1003-5) Available as R-PE conjugate (MON 1004R - 100 tests) Use: WB, C Use: C, F, IF Quantity: 100 µg Quantity: 1 ml CD4, Helper T-cells CD3 CD4, conjugated CD5, rat Cat.no. MON 1036 (Monoclonal) Cat.no. MON 1017 (Monoclonal) Clone BL-TH 4 Cat.no. MON 2048F/R/A (Monoclonal) NEW Cat.no. MON 2022 (Monoclonal) Clone WT32 The antibody reacts with a 59 kD glycoprotein specific for the CD4 The antibody reacts with the T-cell specific antigen CD3. Binding of WT32 antigen, which functions as a receptor for MHC class II and for HIV. Clone MEM-241 Clone 2-4-2B1 to T-cells is blocked by OKT3 and immunoprecipitation with WT32 Human CD4 antigen is a trans membrane glycoprotein (59 kDa) of the The antibody recognizes CD5 of rats. yields a pattern of bands similar to that obtained with OKT3. WT32 is Use: C, F, IF immunoglobulin supergene family present on subset of T-lymphocytes mitogenic for human T-cells, it inhibits T-cell mediated cytotoxicity, and Quantity: 1 ml (“helper/inducer” T-cells) and also expressed at a lower level on Use: F, C can induce non-specific cytotoxicity. monocytes and granulocytes. MAb MEM-241 exhibits superior perfor- Quantity: 100 µg mances in cytofluorometric analysis of cells and is excellent for the Use: IF, MC CD4, Helper T-cell detection of CD4 by Western blotting (under non-reducing conditions). Quantity: 1 ml (MON 1017-1) CD6, pan T-cell 5 ml (MON 1017-5) Use: C Cat.no. MON 1100 (Monoclonal) Quantity: 100 tests Available as FITC conjugate (MON 2048F - 100 tests) Cat.no. MON 1037 (Monoclonal) CD3 Clone MEM-115 Available as R-PE conjugate (MON 2048R - 100 tests) The antibody reacts with a 59 kD glycoprotein specific for the CD4 Available as APC conjugate (MON 2048A - 100 tests) Clone BL-TP 6a antigen, which functions as a receptor for MHC class II and for HIV. The The antibody reacts with a 120 kD glycoprotein specific for the CD6 Cat.no. MON 1080 (Monoclonal) antigen is characteristic for helper T-cells. The specificity was confirmed antigen. CD6 is present on normal mature T-lymphocytes and a by testing with CD4 cDNA transfected cells, with purified CD4 antigen CD5, pan T-cell subpopulation of B-cells in peripheral blood. Clone BL-A8 and competition studies with other CD4 antibodies. The antibody reacts with the T-cell receptor α/β-complex antigen on Use: C, F, IF CD3. The antibody reacts with the majority of the mature peripheral Use: IP, FS, C Cat.no. MON 1064 (Monoclonal) Quantity: 1 ml T-lymphocytes and approx. 35% of the thymocytes. Although the Quantity: 100 µg antibody reacts in fluorescence only with α/β-cells, in a cellular Clone BL-TP 5a(3F4) cytotoxicity test the antibody appeared also to react with α/δ-cells. The antibody reacts with a 67 kD molecule specific for the CD5 antigen. CD7, pan T-cell CD4, Rat The antigen is present on the majority of circulating and non-circulating Use: C, F, IF T-cells as well as on the majority of cortical and medullary thymocytes. Quantity: 1 ml The antigen is also present on a subpopulation of B-lymphocytes. In Cat.no. MON 1006 (Monoclonal) Cat.no. MON 2021 (Monoclonal) histology the antibody stains in lymphoid tissues primarily mature T-lymphocytes and a small subpopulation of B-lymphocytes. Clone WT1 Clone 15-8A2 This antibody reacts with the 40 kD antigen CD7 which is present on The antibody recognizes CD4 of rats. Use: C, F, IF human thymocytes and the majority of peripheral T-cells and NK-cells. Quantity: 1 ml WT1 strongly binds to activated T-cells and thymic T-blasts. CD7 is Use: F, C considered to be an important marker of lymphoblastic leukaemia’s. Quantity: 100 µg Use: F, IF Quantity: 1 ml (MON 1006-1) 5 ml (MON 1006-5)

22 For research use only. Not for drug, diagnostic or other use. www.monosan.com 23 Cluster Differentiation Markers Cluster Differentiation Markers

CD8, Cytotoxic/Suppressor T-cell CD8, conjugated CD9, Platelet, Monocyte, precursor B-cells CD10, CALLA, conjugated

Cat.no. MON 1101 (Monoclonal) Cat.no. MON 1102F/R (Monoclonal) NEW Cat.no. MON 1113 (Monoclonal) Cat.no. MON 1184B/F/R/T (Monoclonal)

Clone BL-Ts 8/2 Clone MEM-31 Clone CLB-47 Clone SJ5-1B4 The monoclonal antibody reacts with the alpha chain of the T-cell The antibody is directed to the human CD8 alpha determinant, a 32 kD The monoclonal antibody is directed against the 24 kD CD9-antigen Expressed on early B- and T-lymphoid precursors, B-blasts, some associated CD8 antigen (gp32), which functions as a receptor for MHC glycoprotein, which is present on a subpopulation of T-cells and on (BA-2-antigen), which is expressed on human B-cell precursors, human granulocytes, bone marrow stromal cells and various epithelia with Class I antigens. This antigen is present on a subpopulation of mature NK-cells. The molecule is involved in T-cell activation and in interactions platelets and human monocytes. The monoclonal antibody reacts with especially high expression on brush border of kidney and gut, some peripheral T-lymphocytes, the cytotoxic/suppressor T-cell subset. with MHC Class I antigens on target cells. early B-cell precursors, pre-pre-B-cells, pre-B-cells, platelets and a smooth muscle and myoepithelial cells, brain cultured fibroblasts and The antigen is also present on ±70% of thymocytes. subpopulation of monocytes. Immunogen: human B-cell precursors certain tumour cell lines. CD10 is widely used as a marker of common Use: C (cALL and ALL). (pre-B) acute lymphocytic leukaemia’s and certain lymphomas. Use: C, F Quantity: 100 tests Quantity: 1 ml Available as FITC conjugate (MON 1102F - 100 tests) Use: C, IF Use: C Available as R-PE conjugate (MON 1102R - 100 tests) Quantity: 1 ml Quantity: 0.5 ml Available as BIOTIN conjugate (MON 1184B - 0.5 ml) CD8, Cytotoxic/Suppressor T-cell Available as FITC conjugate (MON 1184F - 0.5 ml) CD8, Cytotoxic/Suppressor T-cell CD10, Common Acute Leukaemia Antigen, Available as R-PE conjugate (MON1184R - 0.5 ml) Available as TRI-COLOR conjugate (MON 1184T - 0.5 ml) Cat.no. MON 1008 (Monoclonal) CALLA Cat.no. MON 1018 (Monoclonal) Clone FK18 Cat.no. MON 1038 (Monoclonal) CD11a, Lymphocyte Functional Antigen-1, The antibody reacts with CD8, the 33 kD T8 antigen present on the Clone WT82 human cytotoxic/suppressor T-cell subset. It strongly blocks the cytolytic The antibody reacts with the T-cell specific antigen CD8, which is present Clone BL-A11 LFA-1 activity of T8-positive CTLs. It also reacts with a subpopulation of Rhesus on the human cytotoic/suppressor T-cell subset. The antibody can inhibit The antibody reacts with the B-cell associated differentiation antigen monkey lymphocytes which is identical to the population of human T8 T-cell mediated cytotoxicity. CD10 (gp 100). This antigen is a neutral endopeptidase and known as Cat.no. MON1020 (Monoclonal) positive cells. the common acute lymphoblastic leukaemia antigen or cALL associated Use: IF, MC Antigen (CALLA). Clone SPV-L7 Use: F Quantity: 1 ml (MON 1018-1) This antibody reacts with the alpha chain of LFA-1, the CD11a/CD18 Quantity: 1 ml (MON 1008-1) 5 ml (MON 1018-5) Use: C, F integrin heterodimer present on human T- and B-lymphocytes, 5 ml (MON 1008-5) Quantity: 1 ml granulocytes and monocytes. The antibody inhibits cell-cell contact dependent functions such as CTL mediated cytolysis. CD8, Rat CD8, Cytotoxic/Suppressor T-cell CD10, Common Acute Leukaemia Antigen, Use: IF, FS Quantity: 1 ml (MON 1020-1) Cat.no. MON 2023 (Monoclonal) CALLA 5 ml (MON 1020-5) Cat.no. MON 1102 (Monoclonal) Clone 15-11C5 Cat.no. MON 1103 (Monoclonal) Clone MEM-31 The antibody recognizes the CD8 of rats. CD11a, Lymphocyte Functional Antigen-1, The antibody is directed to the human CD8 alpha determinant, a 32 kD Clone MEM-78 glycoprotein, which is present on a subpopulation of T-cells and on Use: F, C The antibody reacts with the B-cell associated differentiation antigen LFA-1 NK-cells. The molecule is involved in T-cell activation and in interactions Quantity: 100 µg CD10. This antigen is a neutral endopeptidase (100 kD glycoprotein) and with MHC Class I antigens on target cells. known as the common acute lymphoblastic leukaemia antigen (CALLA) Cat.no. MON 1025 (Monoclonal) or cALL associated antigen. Use: IF, C, IP CD9, Platelet, Monocyte, precursor B-cell Clone 87-6F9 Quantity: 100 µg Use: IF, C, IP, WB CD11a is expressed on lymphocytes, granulocytes, monocytes and Also available as FITC conjugate (MON 1102F - 100 tests) Quantity: 100 µg macrophages with increase in levels on memory T-cells. CD11a or LFA-1 Also available as R-PE conjugate (MON 1102R - 100 tests) Cat.no. MON 1141 (Monoclonal) has a key role in mediating leukocyte adhesion to endothelium during inflammatory responses through binding to ICAM-1 (CD54). Other Clone M-M2/57 ligands are ICAM-2 and ICAM-3. It is also involved in many T-cell The antibody reacts with the 25 kD molecule of the platelet cell surface. functions and immune phenomena. Mol. weight in SDS gels is about It is a marker for non T-acute lymphoblastic leukaemic cells and acute 180 kD. Part of heterodimer with CD18 (integrin β-2). A defect in β-2 myeloid and chronic lymphoid leukaemia’s. Cross reactivity has been expression results in leucocytes adhesion deficiency (LAD) in which demonstrated by immunofluorescence on rabbit platelets and fibroblasts, patients suffer from recurring bacterial infections. Source: A BALB/c human keratinocytes and MDCK-cell. A 25 kD band is obtained by mouse was immunized and SP2/0 cells were used for fusion with immunoprecipitation of lysates of 36S-labelled human keratinocytes, splenocytes. rabbit fibroblasts and MDCK-cells. Antigen used: human platelet membrane. Use: F, C Quantity: 100 µg Use: F Quantity: 2 ml

24 www.monosan.com For research use only. Not for drug, diagnostic or other use. 25 Cluster Differentiation Markers Cluster Differentiation Markers

CD11a, Lymphocyte Functional Antigen-1, CD11a, Lymphocyte Functional Antigen-1, CD11b, CR3 CD11c, gp150.95 alpha chain, conjugated LFA-1 LFA-1 Clone BL-M/G 1 Cat.no. MON 1105A (Monoclonal) Cat.no. MON 2106F/R (Monoclonal) Cat.no. MON 1039 (Monoclonal) Cat. no. MON 1151 (Monoclonal) Clone BL-M/G 2 Cat.no. MON 1105B (Monoclonal) Clone BL-M/G 4 Cat.no. MON 1105C (Monoclonal) Clone BU15 Clone BL-LEUK 11a Clone MEM-83 The antibody recognizes the CD11c antigen. CD11c is a 150 kDa The antibody reacts with a 180 kD molecule specific for the CD11a This antibody reacts recognizes an unique epitope on CD11a antigen The antibodies react with the CR3 alpha chain of the CD11b/CD18 glycoprotein that associates with CD18 to form the CD11c/CD18 antigen, the alpha chain of the CD11a/CD18 integrin heterodimer. This (LFA-1 alpha chain) implicated in activation of LFA-1 complex. (p165, 95) integrin heterodimer present on human NK-cells, monocytes adhesion complex. CD11c is expressed on granulocytes, monocytes antigen is also known as leucocyte functional antigen or LFA-1, it is the and granulocytes. The antigens function as the C3Bi receptor. The and macrophages, NK-cells, T-cells, and subset of B-cells. ligand of ICAM-1. Use: C, FS antibodies react strong with monocytes, weaker with granulocytes, also Quantity: 100 µg a subpopulation of LGL/NK-cells is stained. Use: C Use: C, F, IF Quantity: 0.5 ml Quantity: 1 ml Use: C, F, IF Available as FITC conjugate (MON 2106F - 0.5 ml) CD11b, CR3 Quantity: 1 ml Available as R-PE conjugate (MON 2106R - 0.5 ml) CD11a, Lymphocyte Functional Antigen-1, Cat.no. MON 1019 (Monoclonal) LFA-1 CD11c, gp150-95 alpha chain CD13 Clone Bear-1 Cat.no. MON 1104 (Monoclonal) This antibody reacts with the CR3 alpha chain of the CD11b/CD18 Cat.no. MON 1106 (Monoclonal) Cat. no. MON 9054 (Monoclonal) NEW (p165, 95) integrin heterodimer present on human NK-cells, monocytes Clone MEM-25 and granulocytes. Clone BL-4H4 Clone WM15 The antibody is directed to the Lymphocyte Functional Antigen-1 (LFA-1). The antibody reacts with the alpha chain of the CD11c/CD18 (gp150-95) The antibody recognizes the human CD13 cell surface glycoprotein, a CD11a, which forms a heterodimer molecule (180-95 kD) consisting of Use: IF, F, IP integrin heterodimer present on human monocytes and granulocytes. 150kD molecule expressed by granulocytes and monocytes, and by the integrin molecules CD11a and CD18. The antigen plays a role in Quantity: 1 ml (MON 1019-1) The antibody reacts strong with monocytes, weaker with granulocytes, myeloid leukaemia cells. CD13 functions as an aminopeptidase enzyme intercellular communication between leukocytes and in adhesion of 5 ml (MON 1019-5) also activated T-cells are stained. In tissue sections the antibody stains and is also a receptor for coronavirus. leukocytes to endothelial cells. The antibody blocks binding of LFA-1 to macrophages. ICAM-1. On endothelial cells the ligand of LFA-1 is ICAM-1 (CD54). Use: C, F, IP, E, FS CD11b, CR3, conjugated Use: C, F, IF Quantity: 100 µg Use: C, IP Quantity: 1 ml Quantity: 100 µg Cat.no. MON1019F/R (Monoclonal) CD13, conjugated CD11c, gp150/95 alpha chain CD11a, LFA-1, conjugated Clone VIM12 This antibody recognizes the CD11b antigen. CD11b associates with Cat.no. MON 1185F/R/T (Monoclonal) CD18 to form the heterodimeric complex known as Mac-1. This complex Cat.no. MON 1011 (Monoclonal) Cat.no. MON 1104F/R (Monoclonal) serves as a receptor for the iC3b component. Mac-1 also serves as an Clone Tük 1 adhesion molecule for intracellular adhesion molecule-1, also known Clone FK24 CD13 is expressed on cells of the myelomonocyte lineage including Clone MEM-25 as CD54. Mac-1 is expressed on cells of the mycloid lineage as well as The antibody reacts with the alpha chain the 150 kD molecule of the myeolomonocyte precursors, monocytes, basophils, eosinophils, The antibody is directed to the Lymphocyte Functional Antigen-1 (LFA-1), natural killer cells. CD11c/CD18 integrin complex (g150/95) which is expressed on neutrophils and myeloid leukaemia’s. The antigen is also present on small CD11a, which forms a heterodimer molecule (180-95 kD) consisting of monocytes and in a lower density on granulocytes and NK-cells of intestine and renal proximal tubule epithelial cells, synaptic membranes the integrin molecules CD11a and CD18. The antigen plays a role in Use: C peripheral blood. It also reacts with many myelomonocytic and of the central nervous system, fibroblasts and osteoclasts. The main intercellular communication between leukocytes and in adhesion of Quantity: 0.5 ml monocytic leukaemia’s and (weakly) with part of the T8 positive cells cellular activity of this antibody is monocytes and granulocytes. Other leukocytes to endothelial cells. This antibody blocks the binding of LFA-1 Available as FITC conjugate (MON1019F - 0.5 ml) (OKM1-like). The integrins are important molecules in cellular adhesion reactive cells in flow cytometry incl. myeloid leukaemia’s and myeloid to ICAM-1. On endothelial cells the ligand of LFA-1 is ICAM-1 (CD54). Available as R-PE conjugate (MON1019R - 0.5 ml) and cellular interactions. cell lines. Other reactive cells in immunohistology incl. histiocytes, high endothelial venules and kidney tubules. Use: C Use: F, IF Quantity: 0.5 ml Quantity: 1 ml (MON 1011-1) Use: C Available as FITC conjugate (MON 1104F - 100 tests) 5 ml (MON 1011-5) Quantity: 0.5 ml Available as R-PE conjugate (MON 1104R - 100 tests) Available as FITC conjugate (MON 1185F - 0.5 ml) Available as R-PE conjugate (MON 1185R - 0.5 ml) Available as TRI-COLOR conjugate (MON 1185T - 0.5 ml)

26 For research use only. Not for drug, diagnostic or other use. www.monosan.com 27 Cluster Differentiation Markers Cluster Differentiation Markers

CD14, Monocyte, Macrophage marker CD14, Monocyte, Macrophage marker CD15, Granulocytes, FITC-conjugated CD16, FcγRIII

Cat.no. MON 1040 (Monoclonal) Cat.no. MON 1135 (Monoclonal) Cat.no. MON 1186F (Monoclonal) clone BL-LGL/1 - Cat. no. MON 1041A (Monoclonal) clone BL-LGL/2 - Cat. no. MON 1041B (Monoclonal) Clone BL-4G7 Clone Tük 4 Clone V1MC6 clone BL-LGL/3 - Cat. no. MON 1041C (Monoclonal) The antibody reacts with a 55 kD molecule specific for the CD14 antigen. CD14 is present on most monocytic and macrophage like cell types: This antibody reacts with X-hapten (3-fucosyl-N-Acetyllactosamine - The CD14 determinant is present on monocytic cells, weakly on monocytes, macrophages, Kupffer cells, pleural phagocytic cells and 3-FAL, 220 kD), that is expressed on >95 % of granulocytes and to a The antibodies react with a 50-70 kD glycoprotein specific for the granulocytes and in tissues also detectable on interdigitating cells. dendritic reticular cells. CD14 is present in low density on a subpopulation varying degree of monocytes. CD16 antigen. CD16 is the low affinity Fc gamma III present on Large granulocytes and activated or transformed B-cells. By flow cytometry, Granular Lymphocytes (LGL), NK-cells, granulocytes and a macrophage Use: F, C the main cellular reactivities are monocytes. Use: C subpopulation. It is a late myeloid differentiation antigen and expressed Quantity: 1 ml Quantity: 100 µg on Chronic Myeloid Leukaemia cells. The antibodies LGL/1 and LGL/3 Use: C show a polymorphism for granulocytes. LGL/1 reacts with granulocytes Quantity: 100 µg of a proportion of the human population and LGL/3 reacts with the CD14, Monocyte, Macrophage marker CD15, Granulocytes, X-Hapten granulocytes of another proportion of the population. The nature of this polymorphism of granulocytes has not yet been revealed. CD14, Monocyte, Macrophage marker Cat.no. MON 1108 (Monoclonal) Cat.no. MON 1109 (Monoclonal) Use: F, C Quantity: 1 ml Clone MEM-18 Cat.no. MON 1072 (Monoclonal) Clone BL-G15 MEM-18 is a well characterized antibody that reacts with the human The antibody reacts with a carbohydrate determinant, X-hapten monocyte marker CD14, a 53 kDa glycoprotein. The CD14 molecule has Clone MEM-15 (3-fucosyl-N-Acetyl-lactosamin), that is present both on glycoproteins CD16, FcγRIII, conjugated been reported to be involved in the endotoxin mediated release of Reacts with soluble and monocyte surface CD14. It does not block the and on glycolipids. The antigen is expressed by all granulocytes and Tumour Necrosis Factor α by monocytic cells. MEM-18 inhibits the binding of LPS-LPS Binding Protein (LPS-LBP) complex to monocytes. It monoblastoid precursor cells. endotoxin induced release of TNF by monocytes because it blocks the will react with both reduced as non-reduced antigen in western blotting. Cat.no. MON 1187F/R/T (Monoclonal) binding of LPS-LBP to monocytes. CD14 is present on most monocytic Use: C, F, IF and macrophage like cell types: monocytes, macrophages, Kupffer cells, Use: C, IP Quantity: 1 ml Clone 3G8 pleural phagocytic cells and dendritic reticular cells. CD14 is present in Quantity: 100 µg The main cellular reactivities are NK-cells, macrophages and granulocytes. low density on a subpopulation granulocytes and activated or trans- formed B-cells. The antibody reacts with both soluble and monocyte CD16 Use: C surface CD14. CD15, Granulocytes Quantity: 0.5 ml Available as FITC conjugate (MON 1187F - 0.5 ml) Use: WB, C, E, FS, IP Cat.no. MON1029 (Monoclonal) Available as R-PE conjugate (MON 1187R - 0.5 ml) Quantity: 100 µg Cat.no. MON 1027 (Monoclonal) Available as TRI-COLOR conjugate (MON 1187T - 0.5 ml) Clone MEM-154 Clone BRA4F1 This antibody reacts on Human CD16 (Fc gamma RIII) expressed mainly CD14, Monocyte, Macrophage marker, BRA4F1 specifically detects CD15. CD15 contains the pentasaccharide on granulocytes and NK-cells, 50-70 kDa. CD16 molecule in granulocytes CDw17, Lactosylceramide lacto-N-fucopentatose III and is particularly expressed on granulocytes is known to display the NA1/NA2 polymorphism. This antibody conjugated and mature neutrophils and on a wide variety of tumour cells including recognizes a polymorphism of CD16A (the NK-cell form), i.e. reacts myeloid leukaemia, breast, colorectal, and lung cancer cells. The mAb very weakly with NK-cells of some subjects. Antibody reacts well with Cat.no. MON 1107 (Monoclonal) Cat.no. MON 1108F/R/T (Monoclonal) BRA4F1 was clustered at the IVth International Workshop on Leucocyte granulocytes (CD16B) of all subjects. Differentiation Antigens. Source: A BALB/c mouse was immunized with Clone MEM-74 Clone Tük 4 K562 (an erythroid-myeloid leukaemia cell line). Splenocytes were fused Use: C, IP, WB, FS The antibody reacts with the human CDw17 antigen, lactosylceramide, CD14 is present on most monocytic and macrophage like cell types: with mouse myeloma SP2.0-Ag14 cells. Quantity: 100 µg which is a glycolipid expressed on granulocytes, thrombocytes and a monocytes, macrophages, Kupffer cells, pleural phagocytic cells and monocyte subpopulation. dendritic reticular cells. CD14 is present in low density on a subpopulation Use: F, P, C, E granulocytes and activated or transformed B-cells. Quantity: 100 µg Use: C Quantity: 100 µg Use: C Quantity: 0.5 ml CD15, Granulocytes Available as FITC conjugate (MON 1108F - 0.5 ml) Available as R-PE conjugate (MON 1108R - 0.5 ml) Available as TRI-COLOR conjugate (MON 1108T - 0.5 ml) Cat.no. MON 1182 (Monoclonal)

Clone MEM-158 The antibody reacts granulocyte antigen CD15.

Use: IP, C Quantity: 100 µg

28 www.monosan.com For research use only. Not for drug, diagnostic or other use. 29 Cluster Differentiation Markers Cluster Differentiation Markers

CD18, β-chain of LFA-1, Mac-1, gp150.95 CD19, B-cell, conjugated CD20, B-cell, conjugated CD21, B-cell subset, C3d receptor, CR2, EBV receptor Cat.no. MON 1110 (Monoclonal) Cat.no. MON 1188B/F/R/T (Monoclonal) Cat.no. MON 1189F/R/T (Monoclonal) Cat.no. MON 1028 (Monoclonal) Clone MEM-48 Clone SJ25-C1 Clone H147 The antibody recognizes the human leucocyte integrin subunit β2, CD18 The CD19 antigen is the most broadly expressed surface marker for CD20 is present on all normal B-cells in peripheral blood, lymph nodes, Clone NZ1 molecule, a 95 kD common beta chain of the CD11/CD18 heterodimer B-cells appearing at the earliest stages of B-cell differentiation. The spleen, tonsils and bone marrow. The antigen is also present on most The antibody reacts with CD21, a 140 kD glycoprotein. The antigen is integrins which are expressed on all leukocytes. CD19 antigen is expressed at all stages of B-cell maturation and is lost B-cell lines and on non-TALL and CLL cells. present on B-cells in sec. follicles of lymph nodes, tonsils and spleen; during terminal B-cell differentiation to plasma cells. some malignant B-cells and on EBV transfected B-cell lines. This antibody Use: C, WB, IP, FS Use: C does not block the biological function of CD21. Quantity: 100 µg Use: C Quantity: 0.5 ml Quantity: 0.5 ml Available as FITC conjugate (MON 1189F - 0.5 ml) Use: F Available as BIOTIN conjugate (MON 1188B - 0.5 ml) Available as R-PE conjugate (MON 1189R - 0.5 ml) Quantity: 1 ml (MON 1028-1) CD18, β-chain of LFA-1, Mac-1, gp150.95, Available as FITC conjugate (MON 1188F - 0.5 ml) Available as TRI-COLOR conjugate (MON 1189T - 0.5 ml) 5 ml (MON 1028-5) Available as R-PE conjugate (MON 1188R - 0.5 ml) conjugated Available as TRI-COLOR conjugate (MON 1188T - 0.5 ml) CD21, CR2 CD21, B-cell subset, C3d receptor, CR2, Cat.no. MON 1110F/R (Monoclonal) EBV receptor CD20, B-cell antigen Clone CLB-LFA-1/1 Cat. no. MON 3089 (Monoclonal) NEW The antibody recognizes the CD18 molecule, a 95 kD Integrin molecule clone BL-B21/1 - Cat. no. MON 1112A (Monoclonal) that forms the common beta chain of the CD11/CD18 heterodimer Cat.no. MON 1178 (Monoclonal) Clone 21B9 clone BL-B21/3 - Cat. no. MON 1112B (Monoclonal) Integrins which are expressed on all leukocytes. The monoclonal antibody 21B9 reacts with human CD21. CD21 also Clone B-Ly1 known as complement receptor 2 (CR2), C3d receptor or EBV receptor The antibodies react with the B-cell differentiation antigen CD21 Use: C The antibody reacts with a 35/37 kD phosphoprotein specific for the is a 140 kD protein. CD21 is a glycosylated type I transmembrane protein (gp140), which functions as a receptor for C3d (CR2) and for EB-virus. Quantity: 0.5 ml CD20 antigen. CD20 is present on all normal B-cells in peripheral blood, consisting on the extracellular face of a serie of 15 or 16 CCP domains. The antigen is present on the majority of the mature peripheral Available as FITC conjugate (MON 1110F - 0.5 ml) lymph nodes, spleen, tonsils and bone marrow. The antigen is also CD21 is the receptor for complement components C3d and iC3b as well B-lymphocytes. In frozen sections of lymphoid tissues the antibodies Available as R-PE conjugate (MON 1110R - 0.5 ml) present on most B-cell lines and on non-TALL and CLL cells. The as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 react with B-lymphocytes and follicular dendritic reticulum cells. specificity was confirmed by reactivity with CD20 cDNA transfected cells (sCD21) was shown to efficiently trigger CD23-signalling pathways in and immunoprecipitation. human monocytes. By inducing release of proinflammatory cytokines and Use: F, C CD19, B-cell upregulating expression of molecules involved in antigen presentation, Quantity: 1 ml Use: F sCD21 modulates critical monocyte functions that may be relevant to Quantity: 1 ml allergic and inflammatory disorders. The monoclonal antibody 21B9 Cat.no. MON 1026 (Monoclonal) reacts with CD21 on B-cells and dendritic cells. The monoclonal antibody CD21, B-cell subset, C3d receptor, CR2, 21B9 is a unique antibody which is also useful to recognize cells with EBV receptor, conjugated Clone BC3 CD20, Mature B-cell low CD21 expression such as a subset of T cells, epithelial cells and The antibody reacts with the CD19 antigen (90 kD), which is expressed astrocytes on all normal B-cells, early B-cells and follicular dendritic cells. The Cat.no. MON 1112R/T (Monoclonal) antibody shows a reactivity of 97-100% with cells of several pre-B-cell Cat.no. MON 1111 (Monoclonal) Use: C, WB and B-cell lines. T-cell and monocytic cell lines are negative. In flow Quantity: 100 µg Clone BU32 cytometrical analysis 70% of human tonsil-, 35% of spleen-, 12% of Clone MEM-97 The antibody reacts with CD21, the 140 kD second complement receptor bone marrow and 10% of peripheral blood lymphocytes are positive. The antibody reacts with a 35 kD phosphoprotein specific for the CD20 (CR2) that is expressed on mature B-cells and follicular dendritic cells. The antibody reacts with non-T acute lymphoblastic leukaemia (non antigen. The specificity was confirmed by reactivity with CD20 cDNA CD21, B-cell subset, C3d receptor, CR2, CD21 functions as a receptor for C3d and EB-virus. T-ALL) and chronic lymphatic leukaemia (CLL) cells, whereas T-ALL and transfected cells, immunoprecipitation and competition studies with acute myelogenous leukaemia cells are negative. Antigen location: other CD20 antibodies. CD20 is present on all normal B-cells in peripheral EBV receptor Use: C membrane. Antigen isolated from: chronic Lymphocytes leukaemia cells. blood, lymph nodes, spleen, tonsils and bone marrow. The antigen is Quantity: 0.5 ml also present on most B-cell lines and on non-TALL and CLL cells. Cat.no. MON 7019 (Monoclonal) Available as R-PE conjugate (MON 1112R - 0.5 ml) Use: F, C Available as TRI-COLOR conjugate (MON 1112T - 0.5 ml) Quantity: 1 ml Use: C, IP Clone 4C5 Quantity: 100 µg This antibody reacts with the CD21 (140 kD) molecule, expressed (moderate) on mature B-cells and (at high density) on follicular dendritic cells (FDC).

Use: C, F, IF Quantity: 1 ml

30 For research use only. Not for drug, diagnostic or other use. www.monosan.com 31 Cluster Differentiation Markers Cluster Differentiation Markers

CD22, B-cells CD22, mature B-cells and early CD24, pre-, intermediate-, mature B-cell CD26 B-progenitor cells Cat.no. MON 1042 (Monoclonal) Cat.no. MON 1119 (Monoclonal) Cat.no. MON 1030 (Monoclonal) Cat.no. MON 1059 (Monoclonal) Clone BL-B3C4 Clone CLB-134 Clone 202.36 The antibody reacts with the CD22 antigen. CD22 is a heterodimer Clone RFB4 The monoclonal antibody is directed against the (45, 55, 65 kD) CD24 202.36 reacts with human CD26 (Dipeptidyl peptidase IV), a 110 kD consisting out of 2 glycoproteins (130 and 140 kD). The molecule is The antigen is a heterodimer composed of two glycoprotein chains of antigen, which is expressed on virtually all B-cells: early B-cell protein which occurs in the cell membrane of T-lymphocyte and is up involved in signal transduction in B-cells activated via surface Ig. CD22 130-140 kD. It is present as both cytoplasmic and membrane antigen precursors, pre-pre-B-cells, pre-B-cells, B-cells, intermediate B-cells, regulated after activation. CD26 is also present on endothelial cells. is in principle a B-lineage antigen, that is present in the cytoplasm of on B-lymphocytes. It is expressed in the cytoplasm of the majority of mature B-cells and some plasmacytoid cells. All these cells are positive The mAb was clustered at the VI WLDA. Source: A BALB/c mouse was progenitor B-cells and on the membrane of the majority of mature B-cells including the early B-progenitor cells. Expression appears early, while plasma cells are negative. The monoclonal antibody reacts with immunized with a T-cell clone. Splenocytes were fused with mouse peripheral B-lymphocytes. Furthermore, it is strongly expressed on at approximately the same stage as the CD19 antigen. Surface expression human granulocytes and thier precursors ( from promyelocytic stage). myeloma NS1-cells. Hairy Cell Leukaemia cells and very weakly on some other leukaemias. of the CD22 antigen by B-lymphocytes is variable. Circulating The monoclonal antibody also reacts with virtually all pre-B-cell lines B-lymphocytes are CD22 positive and follicular mantle and marginal and Burkitt cell lines, but the antigen is only expressed on 50% of the Use: C, IP Use: F, C zone B-lymphocytes show strong binding of this monoclonal. Germinal B-cell lymphoblatoid cell lines. Non T-ALL, B-cell NHL and 50% of Quantity: 100 µg Quantity: 1 ml centre B-cells exhibit only weak expression of the CD22 antigen. The myelomas as well as a subpopulation of AML are found to be positive. CD22 antigen is poorly expressed by non T-ALL cells and by a proportion Hairy Cell Leukaemia is found to be weakly positive. of B-CLL-cells. CD27, Activated T-cell antigen CD22, B-cells Use: IF Use: C, F Quantity: 1 ml Quantity: 2 ml Cat.no. MON 1162 (Monoclonal) Cat.no. MON 1067 (Monoclonal) CD25, IL2R α-chain Clone LT27 Clone MEM-01 CD23, IgE, Fcε RII The antibody precipitates a molecule of MW 55 kDa (110 kDa in The antibody reacts uniquely with the β-chain of CD22 antigen. It non-reduced conditions) designated the CD27 antigen. This antigen western blotting it recognizes the 140 kD CD22α whereas it is negative clone BL-D/D1 - Cat.no. MON 1044A (Monoclonal) is expressed on activated T-lymphocytes and belongs to a family of for CD22β. The specificity has been determined by flow cytometry with Cat.no. MON 1043 (Monoclonal) clone RO/G10 - Cat.no. MON 1044B (Monoclonal) proteins associated with the nerve growth factor receptor. Cd27 is PBL and several cell lines, western blotting and competition with a CD22 expressed by thymocytes and peripheral T-cells, and also by EBV standard. Clone BL-C/B8 The antibodies react with the alpha chain of the Interleukin-2 Receptor transformed B-cell lines. The antibody reacts with the B-cell differentiation antigen CD23, (55 kD glycoprotein). CD25 is expressed on activated T-lymphocytes Use: C, WB, IP a 45-50 kD glycoprotein. This antigen acts as a low affinity IgE receptor and B-lymphocytes. The antibody RO/G10 (Cat.no. MON 1044B) reacts Use: C Quantity: 100 µg (Fc epsilon R). The antibody reacts with a subpopulation of earlier and shows earlier a strong signal on activated T-cells than the Quantity: 100 µg B-lymphocytes in peripheral blood and also in frozen sections of lymphoid antibody BL-D/D1 (Cat.no. MON 1044A). tissues. In lymphoid tissues the antibody stains also a fraction of CD22, B-cells, conjugated follicular dendritic reticulum cells and on activated B-lymphocytes a Use: C, F CD27, mature T-cell, B-cell subset higher fraction of positive cells is found. Quantity: 1 ml

Cat.no. MON 1042F/R/T (Monoclonal) Use: F, C Cat.no. MON 1114 (Monoclonal) Quantity: 1 ml CD25, IL2R α-chain, conjugated Clone RFB4 Clone CLB-27 The antibody reacts with the CD22 antigen. CD22 is a heterodimer The monoclonal antibody is directed against the CD27 antigen, a 55 kD consisting out of 2 glycoproteins (130 and 140 kD). The molecule is CD23, IgE, Fcε RII, FITC-conjugated Cat.no. MON 1190F/R/T (Monoclonal) protein, which is expressed on a large subpopulation (75%) of peripheral involved in signal transduction in B-cells activated via surface Ig. CD22 blood T-lymphocytes and most medullary thymocytes. Little binding is in principle a B-lineage antigen, that is present in the cytoplasm of Clone CD25-3G10 was observed with normal human B-cells, CD3 negative leukemic cells, progenitor B cells and on the membrane of the majority of mature Cat.no. MON 1043F (Monoclonal) The antibody reacts with the α-chain of the Interleukin-2 Receptor B-cells derived and myeloid leukaemias. Immunogen: E-rosette positive peripheral B-lymphocytes. Furthermore, it is strongly expressed on (55 kD glycoprotein). CD25 is expressed on activated T-lymphocytes human lymphocytes. Hairy Cell Leukaemia cells and very weakly on some other leukaemias. Clone Tü 1 and B-lymphocytes. The antibody reacts with the B-cell differentiation antigen CD23, Use: C, IF Use: F, C (+Fitc IF) a 45-50 kD glycoprotein. This antigen acts as a low affinity IgE receptor Use: C Quantity: 1 ml Quantity: 0.5 ml (Fc epsilon R). The antibody reacts with a subpopulation of Quantity: 0.5 ml Available as FITC conjugate (MON 1042F - 0.5 ml) B-lymphocytes in peripheral blood and also in frozen sections of lymphoid Available as FITC conjugate (MON 1190F - 0.5 ml) Available as R-PE conjugate (MON 1042R - 0.5 ml) tissues. In lymphoid tissues the antibody stains also a fraction of Available as R-PE conjugate (MON 1190R - 0.5 ml) Available as TRI-COLOR conjugate (MON 1042T - 0.5 ml) follicular dendritic reticulum cells and on activated B-lymphocytes a Available as TRI-COLOR conjugate (MON 1190T - 0.5 ml) higher fraction of positive cells is found.

Use: C, F Quantity: 100 µg

32 www.monosan.com For research use only. Not for drug, diagnostic or other use. 33 Cluster Differentiation Markers Cluster Differentiation Markers

CD28 CD29 CD31, PECAM-1 CD31, Platelet GPIIa’, PECAM-1

Cat.no. MON 1079 (Monoclonal) Cat.no. MON 1078 (Monoclonal) Cat.no. MON 1149 (Monoclonal) Cat.no. MON 1142 (Monoclonal)

Clone 204.12 Clone Moon-4 Clone MEC7.46 Clone VM64 204.12 remained unassigned at the VI international workshop on human Moon-4 is specific for CD29, an adhesion receptor and co stimulatory The antibody reacts with the mouse form of the Platelet-Endothelial The monoclonal antibody is directed against the CD31 antigen, a 140 kD leukocyte differentiation antigens. Source: A BALB/c mouse was molecule. This antigen was initially characterized as gpIIa on platelets Cell Adhesion Molecule. The reactivity of the antibody is restricted to protein, which is expressed on human platelets and endothelial cells immunized with stimulated human leucocytes. Splenocytes were fused and as the common β1 subunit of the very late antigen (VLA) protein the isoform of the molecule that is selectively expressed by endothelial and is designated as PECAM-1 (platelet-endothelial cell adhesion with NS1. family. CD29 forms a non-covalent heterodimeric complex with integrin-α cells. The antibody precipitates a 130 kDa molecule present on the molecule). The antibody reacts with platelets, monocytes/macrophages, subunits. It also mediates adhesion to invasin and thrombospondin. membrane of endothelial cell presents on all mouse blood vessels both granulocytes and B-cells. It stains the cell boundaries of endothelial Use: F, C Source: A BALB/c mouse was immunized. After three weeks the mouse in normal and inflamed or tumour tissues. The antigen is predominantly cells with a staining pattern identical to that of silver staining. Quantity: 100 µg was boosted and the splenocytes fused with myeloma cell line NSO. present at the lateral borders of endothelial cells as described for human Furthermore in histology it stains weakly histocytes and glomeruli. A positive hybridoma obtained after three limiting dilutions served as PECAM-1. source for the hybridoma. Use: F, IF, WB CD28 Use: IP, C Quantity: 1 ml Use: F Quantity: 100 µg Quantity: 100 µg Also available as BIOTIN conjugate (MON 1149B - 50 µg) Cat.no. MON 1183 (Monoclonal) CD32, FcγRII, R-PE-conjugated Clone YTH 913.12 CD29, Integrin β1 chain CD31, PECAM-1 The antibody reacts with CD28 transfectants and has been clustered Cat.no. MON 1152R (Monoclonal) as a CD28 at the 4th leukocyte workshop in Vienna. Functionally it enhances the MLR response. It reacts with an antigen of 41 kD. This Cat.no. MON 1171 (Monoclonal) Cat.no. MON 1199 (Monoclonal) Clone C1KM5 antibody has been shown to enhance T-cell responses such as the MLR. The antibody reacts with proteins coded by a group of closely related In general CD28 antibodies are most effective at activating cells when Clone TDM29 Clone BV8 genes which show alternative splicing. All forms are present on used in combination with other T-cell markers, particularly CD2. The antigen is a membrane glycoprotein (130 kDa) that can complex with This monoclonal antibody (subclass IgG1) binds to human PECAM-1 monocytes, the FcγRIIB forms are present on B-lymphocytes, and the

Immunogen: human peripheral blood T-cells. the 6 different α-chains of the VLA’s. It is also known as VLA β1-chain (platelet endothelial cell adhesion molecule-1; CD31) a specific of FcγRIIA and C-forms are found on neutrophils. FcγRII proteins are also and as fibronectin receptor. It is not expressed on erythrocytes and endothelial cell junctions. It is also expressed in platelets and leukocytes. found on placental trophoblasts and endothelial cells. In transfected Use: C, F, IF, FS only weakly stains granulocytes. mouse B-cell line BHK-21 it reacts with FcγRIIA (isoforms-LR and -HR) Quantity: 2 ml Use: E, IP, C and FcγRIIB (isoforms -1 and -2). In transfected mouse B-cell line Use: IP, C Quantity: 100 µg HAI.6 it reacts with FcγRIIA (isoforms-LR and -HR). Quantity: 1 ml CD29 Use: C CD31, Platelet GPIIa’, PECAM-1 Quantity: 100 µg CD30, HSR-cells, activated lymphocytes Cat.no. MON 1075 (Monoclonal) Cat.no. MON 1116 (Monoclonal) CD32 Clone MEM-101A Cat.no. MON 1115 (Monoclonal) Human CD29 antigen (integrin beta-1 subunit), broadly expressed on Clone CLB-HEC/75 many cell types, 130 kDa. Clone Ki 1 The monoclonal antibody is directed against the CD31 antigen, a 140 kD Cat.no. MON4038 (Monoclonal) NEW The monoclonal antibody is directed against the 105-120 kD CD30 protein, which is expressed on human platelets. The antibody reacts Use: C, IP, WB antigen, which is expressed on Hodgkin an Sternberg-Reed (HSR) cells with platelets, monocytes/macrophages, granulocytes and B-cells. It Clone AT10 Quantity: 100 µg in Hodgkin disease. The monoclonal antibody also reacts with activated stains the cell boundaries of endothelial cells with a staining pattern AT10 recognizes the human CD32 antigen, a 40kD glycoprotein that lymphocytes but does not react with resting lymphocytes. identical to that of silver staining. Furthermore, in histology it stains acts as a low affinity receptor for IgG (also known as Fc gamma RII). weakly histocytes and glomeruli. Immunogen: human endothelial cells. The antigen mediates several functions including endocytosis, activation Use: F, IF of secretion, cytotoxicity and immunomodulation. CD32 is expressed Quantity: 2 ml Use: F, IF by B cells, monocytes, granulocytes and platelets. Quantity: 1 ml Clone AT10 blocks the binding of IgG to Fc gamma R11

Use: C, F, IP Quantity: 200 µg Also available as BIOTIN conjugate (MON 4038B - 100 µg) Also available as FITC conjugate (MON 4038F - 100 µg)

34 For research use only. Not for drug, diagnostic or other use. www.monosan.com 35 Cluster Differentiation Markers Cluster Differentiation Markers

CD33, pan Myeloid, conjugated CD34, endothelial cell, conjugated CD35, C3b receptor, CR1 CD36, Platelet GPIV, Thrombospondin Receptor Cat.no. MON 1191F/R/T (Monoclonal) Cat.no. MON 1192B/R (Monoclonal) Cat.no. MON 1117 (Monoclonal) Cat.no. MON 1118 (Monoclonal) Clone CD33-4D3 Clone ICH-3 Clone E11 The monoclonal antibody reacts with human CD33. The CD33 antigen The antibody recognizes the CD34 antigen, some cross reactions with The monoclonal antibody is directed against the 250 kD and 220/25 kD Clone CLB-IVC7 is expressed on monocytes, myeloid progenitor cells and mast cells. vascular associated adventitia and some basement membranes. Ultra CD35 antigen, which has been demonstrated to be CR1, the receptor The monoclonal antibody is directed against the CD36 antigen (a 90 kD structures studies, using an indirect immuno-gold technique on ultra for complement component C3b. The monoclonal antibody reacts with glycoprotein). The antigen is also called GP IV or GP IIIb. It is expressed Use: C thin sections of human umbilical artery, have revealed that this antigen divers cell types, like erythrocytes, B-lymphocytes, monocytes, on human platelets. The monoclonal antibody reacts with platelets, Quantity: 0.5 ml is associated to a degree with the basoluminal surfaces of endothelium granulocytes and kidney podocytes. monocytes, macrophages, erythroblasts and weak with B-cells. In Available as FITC conjugate (MON 1191F - 0.5 ml) and shows a slight reaction with an intracytoplasmic vesicular immunohistology the monoclonal antibody reacts with some endothelial Available as R-PE conjugate (MON 1191R - 0.5 ml) compartment. Predominant staining on the endothelial cell membrane. Use: F, IF, P cells, adipocytes and the granular layer of the skin. The GP IV (GP IIIb) Available as TRI-COLOR conjugate (MON 1191T - 0.5 ml) Quantity: 1 ml molecule has been shown to function as thrombospondin receptor on Use: C platelets and monocytes and as a receptor for Plasmodium falciparum Quantity: 0.5 ml infected erythrocytes. Immunogen: human monocytes. CD34 Available as BIOTIN conjugate (MON 1192B - 0.5 ml) CD36 Available as R-PE conjugate (MON 1192R - 0.5 ml) Use: F, IF Quantity: 1 ml Cat.no. MON 1198 (Monoclonal) Cat. no. MON 3084 (Monoclonal) NEW CD34, endothelial cell Clone ICO-115 Clone FA6-152 CD36, Platelet GPIV, Thrombospondin ICO-115 detects CD34 selectively expressed on haemopoietic progenitor Monoclonal antibody FA6-152 reacts with CD36 (88-kDa), a cell surface cells, vascular endothelium and some tissue fibroblasts. CD34 is a trans Cat.no. MON 1164 (Monoclonal) class B scavenger receptor. CD36 is a heavily N-glycosylated trans- Receptor membrane phosphoglycoprotein likely to be involved in cellular signal membrane protein of 90 kDa with two short intracellular domains and transduction and in mediation of intercellular adhesion. ICO-115 have Clone QBEND-10 a large extracellular domain. CD36 expression is primarily controlled Cat.no. MON 1143 (Monoclonal) been characterised on the VIth international workshop on human The antibody recognizes the CD34 antigen, some cross reactions with by the transcription heterodimer PPARg-RXR (peroxisome proliferator- differentiation antigens. Source: A BALB/c mouse was immunized with vascular associated adventitia and some basement membranes. Ultra activated receptor-g-retinoid-X-receptor). CD36 is preferentially found Clone VM58 blast cells of a chronic myeloid leukaemia patient and the isolated structure studies, using an indirect immuno-gold technique on ultra within lipid rafts, which facilitates its association with receptors, The monoclonal antibody VM58 is directed against the CD36 antigen, splenocytes were fused with NS1-cells. thin sections of human umbilical artery, have revealed that this antigen signalling and adaptor molecules. CD36 is expressed on platelets, a 90kD glycoprotein, also called GP IIIb expressed on human platelets. is associated to a degree with the basoluminal surfaces of endothelium mature monocytes and macrophages, microvascular endothelial cells, The GP IV (GP IIIb) molecule has been shown to function as throm- Use: F, C and shows a slight reaction with an intracytoplasmic vesicular mammary endothelial cells, during stages of erythroid cell development bospondin receptor on platelets and monocytes and as receptor for Quantity: 100 µg compartment. Predominant staining is on the endothelial cell membrane. and on some macrophage derived dendritic cells. CD36 has been Plasmodium falciparum infected erythrocytes. The monoclonal implicated in many biological processes including angiogenesis, antibody reacts with platelets, monocytes, macrophages, erythroblasts QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110 kD phagocytosis, inflammation, and lipid and glucose metabolism. CD36 and (weakly) with B-cells. In immunohistology the monoclonal antibody CD34, Mouse expressed on haematopoietic cells and on the established myeloid is associated with Src-family kinases and with the integrins a3b1 and reacts with some endothelial cells, adipocytes and the granular layer leukemic cell line KG1A. Its target epitope is distinct from those of a6b1. It is a receptor and transporter of oxidized lipids and long chain of the skin. The antibody VM58 induces Fc-receptor-dependent platelet other anti-CD34 reactive mAbs as demonstrated by its lack of sensitivity fatty acids. CD36 has been shown to function as phagocytic receptor aggregation after binding to platelet. The heterogeneity of the response Cat.no. MON 1159 (Monoclonal) to neuraminidase treatment, and fluorescence staining profiles during for apoptotic cells. Several in vivo models support the role of the found in healthy individuals is dependent on the polymorphism of the flow cytometry analysis of target cell populations. The anti CD34 thrombospondin / CD36 system in angiogenesis and tumor growth. Fc RII on platelets. Clone MEC 14.7 reactivity of QBEND-10 was established in a molecular test for specificity, An important role for CD36 has been found in Malaria as major receptor The antibody reacts with Mouse CD34, a protein present on endothelial the results are published in the proceedings of the 4th International for P. falciparum-infected red blood cells. Many different ligands have Use: F, IF cells and hematopoietic progenitor cells. The antibody recognizes a Workshop on leukocyte Antigens. Antigen used: human endothelial been reported to interact with CD36, suggesting that CD36 could Quantity: 1 ml neuraminidase sensitive epitope on endothelium in vivo, particularly vesicles. Acetone fixed rat lung endothelial cell (90% RECA positive) recognize a structure-based domain rather than specific contact residues. on small vessels and neoformed capillaries and developing vascular stain negative with this antibody. Also this antibody does not cross Monoclonal antibody FA6-152 blocks the biological activity of CD36 structures in embryonal structures. Furthermore the antibody reacts react with tested species such as sheep, cow, dog and rat. Immunogen: by blocking collagen/thrombospondin binding. with hematopoietic progenitors particularly of myelomonocytic colony human endothelial vesicles. forming cells. Use: IP, E, C, WB, F Use: C, F, P Quantity: 100 µg Use: C, F, IF, IP, E, WB Quantity: 2 ml Quantity: 100 µg

36 www.monosan.com For research use only. Not for drug, diagnostic or other use. 37 Cluster Differentiation Markers Cluster Differentiation Markers

CD37 CD39 CD41, GPIIb/IIIa complex CD41a, Platelet GPIIb/ IIIa complex, alphaIIb/beta3-integrin Cat.no. MON 1035 (Monoclonal) Cat. no. MON 4026 (Monoclonal) NEW Cat.no. MON 1120 (Monoclonal) Cat.no. MON 1145 (Monoclonal) Clone IPO-24 Clone HIM80 Clone CLB-48 The antigen CD37 defined by IPO-24 is B-lineage restricted and is This monoclonal antibody reacts with the CD39 antigen. Apparent molecular weight under reducing conditions: GPIIIa; 110 kD; Clone CRC64 strongly expressed throughout B- cell ontogeny from pre-B cells until GPIIb alpha chain; 125 kD, beta chain; 25 kD. The monoclonal antibody The monoclonal antibody recognizes the intact platelet GPIIb/IIIa

the plasmoblasts. CD37 is an extensively glycosylated molecule and Use: P, F, C recognizes the intact platelet GPIIb/IIIa complex, i.e. it does not bind to complex (alphaIIb-/beta3-integrin), i.e. it does not bind to dissociated usually two and sometimes three N-linked carbohydrate chain are Quantity: 100 µg dissociated GPIIb or GPIIIa. The antibody reacts with human trombocytes GPIIb or GPIIIa. The antibody reacts with human platelets and attached to the protein. IPO-24 reacts with human B-cell lines Daudi, and megakaryocytes. The antibody does not bind to platelets from megakaryocytes. Apparent molecular weight under reducing conditions: Raji, Namalva, Ramos and CABIL, whereas T-cell lines CEM, Molt-4, patients with Glanzmann thrombasthenia and does not recognize the GPIIIa; 110 kD; GPIIb alpha chain; 125 kD, beta chain; 25 kD. The Jurkat, MT-4, H9 and non-lymphoid cell lines MeWo and A-431 are not CD40, B-cells vitronectin receptor, which contains GPIIa as a beta subunit (cf. CD61). antibody does not bind to platelets from patients with Glanzmann labelled. IPO-24 stains B-cell follicles and the most strongly labelled The monoclonal antibody activates human thrombocytes and induces thrombasthenia and does not recognize the vitronectin receptor, which elements are mantle zone lymphoid cells. Neutrofils, macrophages, thrombocyte aggregation in the absence of trombocyte inhibitors such contains GPIIIa as a beta subunit (cf. CD61). CRC64 (both intact antibody

thymocytes and dendritic reticulum cells are negative. Source: IPO-24 Cat.no. MON 1046 (Monoclonal) as EDTA. Immunogen: human platelets. and F(ab’)2 fragments) inhibits platelet aggregation by blocking was generated after immunization of BALB/c mice with spleen cells of fibrinogen binding to the glycoprotein IIb-IIIa complex. a patient with hairy cell leukaemia. Mice splenocytes were fused with Clone BL-C4 Use: IF the P3-X63-Ag8.653 mouse myeloma cell line. The antibody reacts with CD40, a 50 kD glycoprotein. CD40 is expressed Quantity: 1 ml Use: C, IF on all mature peripheral B-lymphocytes. In tissue sections it reacts with Quantity: 1 ml Use: F, P, C, E B-lymphocytes and with interdigitating reticulum cells. Quantity: 100 µg CD41a Use: C, F CD41a, Platelets GPIIb/IIIa complex, Quantity: 1 ml alpha /beta -integrin CD38, T10 Cat. no. MON 4027 (Monoclonal) NEW IIb 3 CD40, FITC-conjugated Clone HIP8 Cat.no. MON 1144 (Monoclonal) Cat.no. MON 1045 (Monoclonal) The HIP8 monoclonal antibody reacts with the human CD41 molecule, the integrin alphaIIb also known as platelet GPIIb. CD41 non-covalently Clone VM16a Clone BL-D2 Cat.no. MON 2146F (Monoclonal) associates with betaIII integrin (GPIIIa, CD61) and is expressed by The monoclonal antibody recognizes the intact platelet GPIIb/IIIa

The antibody reacts with a 45 kD molecule specific for the CD38 (T10) megakaryocytes and platelets. The CD41/CD61 complex is a receptor complex (alphaIIb-/beta3), i.e. it does not bind to dissociated GPIIb or antigen. CD38 is present on NK-cells (LGL), and a subpopulation of Clone HB14 for fibronectin, fibrinogen, von Willebrand factor, vitronectin and GPIIIa. The antibody reacts with human platelets and megakaryocytes. T- and B-lymphocytes in peripheral blood. Furthermore it is expressed The antibody reacts with CD40. The CD40 antigen is a member of a thrombospondin and mediates platelets aggregation. HIP8 blocks Apparent molecular weight under reducing conditions: GPIIIa; 110 kD; by hematopoietic precursor cells, thymocytes, plasma cells and activated receptor family that includes CD27, CD30, Fas, the low affinity Nerve platelet aggregation. CD41 (clone HIP8) completely inhibits ADP-, GPIIb alpha chain; 125 kD, beta chain; 25 kD. The antibody does not T- and B-lymphocytes. CD38 is also seen on ALL, B-cell progenitor lines Growth Factor Receptor (NGFR), Tumor Necrosis Factor Receptors epinephrine-, and collagen-induced platelet activation, and partially bind to platelets from patients with Glanzmann thrombasthenia and and on cell lines obtained from Burkitt lymphoma. (TNFR: I & II), as well as others. This antibody is a pan-B cell marker. inhibits ristocetin- and thrombin-induced platelet activation. This does not recognize the vitronectin receptor, which contains GPIIIa as a The CD40 antigen is also expressed on interdigitating cells (IDC), basal antibody is useful in the morphological and physiological studies of beta subunit (cf. CD61). VM16a inhibits platelet Fc-receptor dependent Use: C, F epithelial cells, and some carcinomas. The CD40 is also expressed, to a platelets and megakaryocytes. The GPIIb/IIIa may be absent or strongly reactions due to the proposed topographical association of the Quantity: 1 ml lesser extent, on follicular dendritic cells, macrophages, and some reduced in Glanzmann’s thrombasthenia (GT). platelet Fc-receptor and the glycoprotein IIb-IIIa complex. epithelial cells. The CD40 antibody has demonstrated the ability to suppress apoptosis. Use: F, C Use: C, IF CD38, conjugated Quantity: 100 µg Quantity: 1 ml Use: C Quantity: 0.5 ml Cat.no. MON 1193B/F/T (Monoclonal) CD41a, Platelet GPIIb/IIIa Clone HIT2 CD38 is present on NK-cells (LGL), and a subpopulation of T- and Cat.no. MON 1173 (Monoclonal) B-lymphocytes in peripheral blood. Furthermore it is expressed by hematopoietic precursor cells, thymocytes, plasma cells and activated Clone M148 T- and B-lymphocytes. CD38 is also seen on ALL, B-cell progenitor lines The antibody is specific to glycoprotein IIb/IIIa complex on platelets, and on cell lines obtained from Burkitt lymphoma. megakaryocytes, and monocytes. Acute megakaryoblastic leukaemia cells are positive for the gpIIb/IIIa antigen whereas common acute Use: C lymphoblastic leukaemia and acute myeloid leukaemia are negative. Quantity: 0.5 ml Available as BIOTIN conjugate (MON 1193B - 0.5 ml) Use: IF Available as FITC conjugate (MON 1193F - 0.5 ml) Quantity: 1 ml Available as TRI-COLOR conjugate (MON 1193T - 0.5 ml)

38 For research use only. Not for drug, diagnostic or other use. www.monosan.com 39 Cluster Differentiation Markers Cluster Differentiation Markers

CD41b CD42d, Platelets GP V CD44, lymphocyte homing receptor, H-CAM CD45, Leukocyte Common Antigen, LCA

Cat. no. MON 4028 (Monoclonal) NEW Cat.no. MON 1121 (Monoclonal) Cat.no. MON 1123 (Monoclonal) Cat.no. MON 1049 (Monoclonal)

Clone HIP2 Clone CLB-SW16 Clone MEM-85 Clone BL-LEUK 45 CD41 (GPIIb) is a calcium-depedent, noncovalently associated hetero- The monoclonal antibody is directed against the 82 kD GP V antigen, The antibody reacts with an 80 kD glycoprotein, the human antigen The antibody reacts with the Leukocyte Common Antigen. Dependent dimer and contains a heavy chain (GPIIb-alpha) and a light chain which is expressed on human platelets. The monoclonal antibody reacts CD44, H-CAM. The CD44 antigen is present on T-lymphocytes, on the cell type involved glycoproteins with 220, 205, 190, 180 and (GPIIb-beta) linked by a single disulfied bond. The HIP2 monoclonal with human platelet. It has been shown that the glycoproteins GP V granulocytes, brain, red blood cells and epithelial cell lines. The antibody sometimes 170 kD are precipitated by this antibody.

antibody reacts with the human CD41 molecule IIb also known as and GPIb-GP IX form a non-covalent complex in the platelet membrane. reacts with the same epitope as Hermes-1 on the molecule, which is a platelet GPIIb. CD41 non-covalently associates with beta3 integrin The monoclonal antibody does not react with lymphocytes, granulocytes, leukocyte adhesion molecule, (hyaluronan receptor), participating at Use: C, IF (GPIIIa, CD61) and is expressed by platelet precursors and platelets monocytes and erythrocytes. Antigen is expressed on human platelets homing. Quantity: 1 ml (megakaryocytes). The CD41/CD61 complex is a receptor for fibronectin, and megakaryocytes. The antigen is absent or present in very low levels fibrinogen, von Willebrand factor, vitronectin and thrombospondin and on platelets of patients with the Bernard-Soulier syndrome. Immunogen: Use: C, F, IF mediates platelets aggregation. The GPIIb/IIIa may be absent or strongly human platelets Quantity: 100 µg CD45, Leukocyte Common Antigen, LCA reduced in Glanzmann’s thrombasthenia(GT). Use: IF, C Use: F, C Quantity: 1 ml CD44, H-CAM, conjugated Cat.no. MON 1179 (Monoclonal) Quantity: 100 µg Clone MEM-28 CD43, Leukosialin Cat.no. MON 1123F/R (Monoclonal) The antibody reacts with human pan-leukocyte antigen CD45; a 170- CD42a, Platelet GPIX 220 kDa glycoprotein. MEM-55 reacts with all alternative forms of CD45. Clone MEM-85 Cat.no. MON 1048 (Monoclonal) The antibody reacts with an 80 kD glycoprotein, the human antigen Use: C, IP, WB, P Cat.no. MON 1047 (Monoclonal) CD44, H-CAM. The CD44 antigen is present on T-lymphocytes, Quantity: 100 µg Clone BL-E/G3 granulocytes, brain, red blood cells and epithelial cell lines. The antibody Clone BL-H6 The antibody reacts with CD43, the glycoprotein Leukosialin (95 and reacts with the same epitope as Hermes-1 on the molecule, which is a The antibody reacts with CD42a, a 23 kD glycoprotein also called gpIX. 115 kD). The antigen is present all T-lymphocytes, most of the leukocyte adhesion molecule, (hyaluronan receptor), participating at CD45, Leucocyte Common Antigen, LCA, The antigen is present on thrombocytes and also on a so far unidentified B-lymphocytes and on granulocytes and monocytes in peripheral blood. homing. subpopulation of T-lymphocytes. In tissue sections T-cells are stained very brightly, also in formalin fixed conjugated and paraffin embedded tissues. Use: C Use: C, F Quantity: 0.5 ml Cat.no. MON 1194F/R/T (Monoclonal) Quantity: 1 ml Use: C, F, P Available as FITC conjugate (MON 1123F - 0.5 ml) Quantity: 1 ml Available as R-PE conjugate (MON 1123R - 0.5 ml) Clone H130 The antibody reacts with the Leukocyte Common Antigen (LCA), also CD42b, Platelet GPIb designated as CD45. CD43, Leukosialin CD44, lymphocyte homing receptor, H-CAM Use: C Cat.no. MON 1146 (Monoclonal) Quantity: 0.5 ml Cat.no. MON 1122 (Monoclonal) Cat.no. MON 1150 (Monoclonal) Available as FITC conjugate (MON 1194F - 0.5 ml) Clone VM16d Available as R-PE conjugate (MON 1194R - 0.5 ml) The monoclonal antibody is directed against the CD42b antigen, platelet Clone MEM-59 Clone NKI-P2 Available as TRI-COLOR conjugate (MON 1194T - 0.5 ml) glycoprotein GPIb, that serves as a receptor for von Willebrand factor The antibody reacts with the human CD43 antigen (gp 95, leukosialin). The antibody reacts with an 90 kD glycoprotein, the human antigen and as a high affinity thrombin receptor. The antigen is expressed on CD43 is a non-lineage antigen that is expressed on several cell types CD44, also designated pgp-1. The CD44 antigen is widely expressed by human platelets and megakaryocytes. The antigen is absent or present such as T-lymphocytes, granulocytes and monocytes. The epitope haematopoietic cells including T-lymphocytes, myeloid cells, macro- CD45RA in very low levels on platelets of patients with the Bernard-Soulier recognized by this antibody is destroyed by neuraminidase. The antibody phages, dendritic cells, red blood cells and brain, epithelial cell lines. syndrome. VM16d inhibits low conc. thrombin (<0.1 U/ml) induced recognizes a sialic acid-dependent epitope (like most other CD43 The CD44 molecule is considered to be the lymphocyte homing receptor, platelet aggregation and decreases thrombin binding to platelets. antibodies). The antibody co-stimulates T-lymphocytes. H-CAM. The antibody blocks adhesion of lymphocytes to high endothelial Cat.no. MON 1050 (Monoclonal)

F(ab’)2 and F(ab’) fragments are equally effective as whole intact The CD43 antibody induces apoptosis of lineage marker negative bone venules. antibodies. The VM16d-epitope is located within the 45 kDa N-terminal marrow hematopoietic progenitor cells that express CD34 at a high Clone BL-4D4 domain of GPIb alpha-chain. VM16d does not effect platelet interaction density (CD34hi LIN-). However stem cells, more primitive cells and Use: F, IF The antibody reacts with CD45RA, the 200 and 220 kD molecules of with von Willebrand factor, leading to the conclusion that the antibody differentiated myeloid and mature lymphocytes do not respond to Quantity: 1 ml the leukocyte common antigen. The antigen is present on peripheral reacts with or nearby the thrombin receptor site. CD43 mediated stimulation by apoptosis. Therefore it appears to be a blood B-lymphocytes and on a subpopulation of T-lymphocytes, the narrow window within the differentiation pathway of stem cells towards CD45RA positive fraction of CD4+ T-cells is the subpopulation of naive, Use: IF mature hematopoietic cells that seems sensitive to apoptosis triggered not activated inducer/suppressor cells. Quantity: 1 ml by CD43. Use: C, F, P Use: C, IP, WB, P Quantity: 1 ml Quantity: 100 µg

40 For research use only. Not for drug, diagnostic or other use. www.monosan.com 41 Cluster Differentiation Markers Cluster Differentiation Markers

CD45RA CD45RB CD46 CD49a

Cat.no. MON 1125 (Monoclonal) Cat. no. MON 2085 (Monoclonal) NEW Cat.no. MON 2000 (Monoclonal) Cat.no. MON4042 (Monoclonal) NEW

Clone MEM-56 Clone MEM-143 Clone MEM-258 Clone TS2/7 The antibody is directed against the 205-220 kD form of CD45, the The antibody reacts with a protein determinant of CD45RB and is This antibody recognizes the human CD46 antigen TS2/7 recognizes the human alpha 1 integrin sub-unit, which forms the leukocyte common antigen. This antigen is present on peripheral therefore not neuraminidase sensitive like most other anti-CD45RB VLA-1 heterodimer in association with the beta 1 integrin. VLA-1 is a B-lymphocytes as well as on a subpopulation of the peripheral antibodies (including standard antibody MEM-55). The reactivity of the Use: WB, IP, C receptor for collagen and laminin, and is expressed by chronically T-lymphocytes. The population of CD4+ T-lymphocytes carrying the antibody MEM-143 can be blocked by a peptide including amino acids Quantity: 100 µg activated T cells, melanoma cells and smooth muscle cells. This clone CD45RA determinant is the non-activated suppressor-inducer subset 79-88. has been reported as being suitable for use in Western Blotting. of CD4 T-lymphocytes. Use: C CD47 Use: C, F, IP Use: C, IP, WB, P Quantity: 100 µg Quantity: 0.5 ml Quantity: 100 µg Cat.no. MON 1165 (Monoclonal) CD45RO, Leukocytes Common Antigen, CD49b, Platelet GP Ia, VLA-2 alpha chain CD45RA, conjugated Clone BRIC 126 LCA, T-cells The CD47 has a very broad tissue distribution. There are approximately 50.000 CD47 molecules per erythrocyte. The glycoprotein is deficient Cat.no. MON 1126 (Monoclonal) Cat.no. MON 1125F/R (Monoclonal) Cat.no. MON 1124 (Monoclonal) in erythrocytes of the rare Rh null phenotype. CD47 is a heavily N-glycosylated cell membrane glycoprotein of apparent molecular weight Clone CLB-150 Clone MEM-56 Clone CLB-UCHL1 47-52 kDa. It is of unknown function suitable as a positive control for The monoclonal antibody is directed against the CD49b antigen, The antibody is directed against the 205-220 kD form of CD45, the The monoclonal antibody is directed against the 180 kD CD45RO-antigen flow cytometry and immunohistochemistry. BRIC 126 was made in molecular weight 130, 170 kD (GP Ia or VLA-2 alpha-chain), which can leukocyte common antigen. This antigen is present on peripheral (restricted T200), a component of the Leukocyte Common Antigen response to human erythrocytes. It has a functional binding affinity for form distinct complexes with either the CD29 antigen (GP IIa or VLA B-lymphocytes as well as on a subpopulation of the peripheral (LCA) complex, which is expressed on human T-lymphocytes (40% of erythrocytes of 2.8 x 108 M-1. It binds to the 47-52 kDa component beta-chain), resulting in the VLA-2 (alpha-2, beta-1) complex which is T-lymphocytes. The population of CD4+ T-lymphocytes carrying the CD4 and 20% of CD8 positive T-lymphocytes), most thymocytes and on immunoblots of erythrocyte membranes under both reducing and expressed on human platelets. The monoclonal antibody reacts with CD45RA determinant is the non-activated suppressor-inducer subset the majority of T-cell malignancies. The monoclonal antibody also reacts non-reducing conditions. BRIC 126 directly haemagglutinates untreated platelets, long-term cultured T-lymphocytes and activated T-lymphocytes. of CD4 T-lymphocytes. with most human granulocytes and monocytes but reacts hardly with and pronase treated normal erythrocytes, but shows weaker reactions In immunohistology the monoclonal antibody reacts with thymocytes, B-lymphocytes, NK-cells or Thrombocytes. Meanwhile, immunostaining with Rh null erythrocytes, reacting only by the indirect anti globulin epithelial cells of a variety of tissues, peripheral nerves, fibroblasts, Use: C, IF on paraffin tissues has indicated that the monoclonal antibody reacts technique or after pronase treatment. osteoclasts, glomerular mesangium and most non-haemopoietic Quantity: 0.5 ml with normal T-lymphocytes, most T-cell related tumours, true histocytic adherent cell lines. Platelet VLA-2 functions as a collagen and laminin Available as FITC conjugate (MON 1125F - 0.5 ml) lymphomas and granulocytic sarcomas but not with B-cell related Use: C, F, WB, IP receptor. Platelet adhesion is inhibited by this antibody. Immunogen: Available as R-PE conjugate (MON 1125R - 0.5 ml) tumours. Immunogen: T-cell line CA1. Quantity: 200 µg human T-lymphocytes.

Use: C, F, P Use: F, IF, WB CD45RB Quantity: 1 ml CD48, leukocyte surface antigen Quantity: 1 ml

Cat.no. MON 1153 (Monoclonal) CD46, Membrane Co-factor Protein, MCP Cat.no. MON 1085 (Monoclonal) CD49b, Platelet GP Ia, Collagen Receptor Clone MEM-55 Clone MEM-102 CD45RB is present on B-cells, a subpopulation of T-cells, monocytes, Cat.no. MON 1134 (Monoclonal) The antibody binds to the human pan-leukocyte GPI-anchored antigen Cat.no. MON 1181 (Monoclonal) macrophages and granulocytes. This antibody reacts with a carbohy- CD48, which is a 45 kD leukocyte surface glycoprotein specific for the drate eptitope of CD45RB, a sialidase sensitive epitope which is Clone J4-48 CD48 antigen, which is closely related to the activation antigen Blast-1. Clone AK7 expressed on most PBL. The antibody is directed against the CD46 antigen, which is expressed CD48 is probably an adhesion molecule (alternative CD2 ligand). It is The antibody recognizes the CDw49b antigen, a human platelet antigen on the leukocytes, thrombocytes, epithelial and endothelial cells. The GPI-anchored. (Mr 170 kD). The antigen is found on platelets, endothelial cells and Use: C, WB, IP, P antigen recognized by CD46 has been shown in the 4th Workshop to approximately 50% of monocytes together with activated T-cells. The Quantity: 100 µg be the Membrane Co-factor Protein (MCP). A soluble MCP is found in Use: C, IP, WB, P gpIa antigen is non-covalently associated with the 130 kD VLA-β-chain Also available as FITC conjugate (MON1153F - 100 tests) semen and human tear film. Quantity: 100 µg (CD29) in the VLA-2 complex.

Use: IF Use: C, IP Quantity: 1 ml Quantity: 1 ml

42 www.monosan.com For research use only. Not for drug, diagnostic or other use. 43 Cluster Differentiation Markers Cluster Differentiation Markers

CD49c CD49e, GP Ic, VLA-5 alpha-chain CD50, pan Leukocyte CD51, Vitronectin Receptor alpha chain, VNR Cat.no. MON 1274 (Monoclonal) Cat.no. MON 1127 (Monoclonal) Cat.no. MON 1129 (Monoclonal) Cat.no. MON 1130 (Monoclonal) Clone MIKd2 Clone CLB-705 Clone BL-LEUK 50 The antibody recognizes the VLA-α3 chain of the VLA-3 complex and The monoclonal antibody is directed against the CD49e-antigen, The antibody reacts with a membrane glycoprotein (gp 140 and 108 kD) Clone NKI-MG is reactive with epithelial tissue and basal renal distal tubules. Reacts molecular weights: 17, 130 kD, (GP Ic or VLA-5 alpha-chain), which which is expressed by all leukocytes. The antibody is directed against the CD51 antigen, alpha chain of with human, other species not tested. can form distinct complexes with either the CD29-antigen (GP IIa or vitronectin receptor (VNR-alpha chain) which forms a complex with: VLA beta-chain), resulting in the VLA-5 (alpha-5, beta-1) complex Use: C, IF - the CD61 antigen (GP IIIa or Beta-3 chain) resulting in the alpha- Use: C, IP which is expressed on human platelets. Platelet VLA-5 functions as a Quantity: 1 ml beta-3 complex, which is expressed on human platelets; Quantity: 1 ml fibronectin receptor and platelet adhesion to fibronectin is inhibited by - the beta-5 integrin, resulting in the alpha-beta-5 complex, which is this antibody. The monoclonal antibody reacts with platelets and expressed on human monocytes and macrophages; monocytes. It reacts weakly with T-cells and granulocytes and with CD50, pan Leukocyte - the beta-1 integrin, resulting in the alpha-beta-1 complex. CD49c various cell types of non-hemopoietic origin. Immunogen: U937. This The platelet alpha-beta-3 complex functions as a vitronectin receptor antibody has also designated clone member NKI-SAM1. and platelet adhesion to vitronectin is inhibited by this monoclonal Cat.no. MON 1068 (Monoclonal) antibody. Immunogen: human melanoma cells. Cat.no. MON4067 (Monoclonal) NEW Use: IF Quantity: 1 ml Clone MEM-171 Use: IF Clone VM-2 Mab MEM-171 recognizes an epitope in the D2 domain of human CD50 Quantity: 1 ml VM-2 recognizes the human CD49c cell surface antigen, also known as (ICAM-3). the alpha-3 integrin and as VLA-3. CD49c is expressed by B lymphocytes, CD49f, VLA-6 alpha-chain, GP Ic The specificity has been determined by flow cytometry with PBL and within normal skin and upon kidney glomeruli. several cell lines and the reactivity with CD50 transfectants. CD52, T- and B-lymphocytes Use: F Cat.no. MON 1128 (Monoclonal) Use: C, IP Quantity: 1 ml Quantity: 100 µg Cat.no. MON 1168 (Monoclonal) Clone CLB-701 The monoclonal antibody is directed against the CD49f antigen, Clone YTH 66.9HL CD49d, VLA-α4 chain molecular weight 30/31, 120 kD (GP Ic or VLA-6 alpha-chain), which CD51/CD61, Vitronectin Receptor alpha chain, This antibody reacts with human T- and B-lymphocytes. This antibody can form distinct complexes with either the CD29 antigen (GP IIa or probably recognizes a carbohydrate determinant present at high density VLA beta chain), resulting in the VLA-6 (alpha-6, beta-1) complex which VNR on mononuclear blood cells (approximately 5 x 108 molecules/cell) Cat.no. MON 1175 (Monoclonal) is expressed on human platelets, or with the beta-4 integrin resulting which may be carried by more than one glycoprotein. A prominent in the alpha-6, beta-4 complex which is expressed on various human Cat.no. MON 1167 (Monoclonal) family of glycoproteins which are CAMPATH-1 positive have specific Clone 44H6 epithelial cells. Platelet VLA-6 functions as a laminin receptor and molecular weights in the range 25-29 kD. Lytic with human and rabbit The antibody reacts with the 150 kDa VLA-α4 chain (lymphocyte homing platelet adhesion to laminin is inhibited by this antibody. The monoclonal Clone 23C6 complement. This antibody binds weakly with protein A. Cross reactive receptor) which is associated with the CD29 molecule in the VLA-4 antibody reacts with platelets, megakaryocytes, T-lymphocytes and 23C6 was assigned to the CD51 cluster at the 4th International with lymphocytes from old world monkeys. complex. Expressed ALL, AML, T- and B-cells, monocytes, thymocytes common acute lymphoblastic leukaemia cells (alpha-6, beta-1). In Workshop Vienna 1989. This antibody recognizes a 110/90 kDa antigen and Langerhans cells. No reactivity with human kidney or brain sections. immunohistology the monoclonal antibody reacts with epithelial cells known as the α-chain of the vitronectin receptor (VNR). 23C6 reacts Use: C, F, WB Immunogen: Hoon pre-B leukaemia cell line. of a variety of tissues, peripheral nerves, micro vascular endothelial cells, with osteoclasts, placenta, melanoma cell lines, some epithelia cells, and Quantity: 0.5 ml placenta cyto- and syncytotrophoblasts. reacts weakly with platelets. Note: 23C6 shows no reactivity on fixed Use: C, IP tissues. The vitronectin receptor mediates cell adhesion to arg-gly-arp Quantity: 1 ml Use: F, IF containing sequences in vitronectin, von Willebrand factor, fibrinogen, CD53, pan Leukocyte Quantity: 1 ml and thrombospondin. Used antigen: human osteclastoma tissue. Crossreactive with rabbit vitronectin receptor. Clinical relevance: Bone resorption modulation; osteoclast identification, receptor purification; Cat.no. MON 1131 (Monoclonal) CD49f, VLA-6 alpha-chain, Laminin receptor malignant melanoma identification. Clone MEM-53 Use: C, F, IP The antibody is a prototypic antibody for the human CD53 pan-leukocyte Cat.no. MON 1176 (Monoclonal) Quantity: 200 µg determinant, a 35-40 kD glycoprotein. Reacts also with the deglyco- sylated antigen. The antibody induces oxidative burst in granulocytes Clone 4F10 and monocytes. The antibody is useful for the detection of CD53 4F10 is specific to the Integrin α-6 chain which is expressed on positive cells (all leukocytes). It can be used for differential diagnosis epithelial and endothelial basement membrane. This VLA α-6 chain, of haematopoietic tumours, sarcomas and melanomas. which consists of a single disulphide linked 120 kDa subunit and a small doublet subunit of 25/30 kDa, is non-covalently associated with Use: C, IP, WB, F

the VLA β-chain in VLA-6 or α6/β1 complex or with integrin-β4 chain Quantity: 100 µg

(α6/β4). The antigen is also expressed on platelets, monocytes, thymocytes and T-lymphocytes.

Use: F, IP Quantity: 1 ml

44 www.monosan.com For research use only. Not for drug, diagnostic or other use. 45 Cluster Differentiation Markers Cluster Differentiation Markers

CD54, Intracellular Adhesion Molecule-1, CD54, ICAM-1, Biotinylated CD56, Neural Cell Adhesion Molecule, CD56, Small Cell Lung Carcinoma, SCLC, ICAM-1 NCAM NCAM Cat.no. PS 018 (Polyclonal) Cat.no. MON 1016 (Monoclonal) Cat.no. MON 9006 (Monoclonal) Cat.no. MON 9010 (Monoclonal) The antibody reacts with both intact human ICAM-1, CD54 and with Clone HM-1 soluble human ICAM-1. The antibody reacts also with ICAM-1 present Clone 123C3 Clone MOC-1 The antibody reacts with the 90 kD glycoprotein Intracellular Adhesion on chimpanzee, rhesus monkey, cynomolgus monkey and baboon tissues. The antibody recognizes a trans membrane glycoprotein of 140 and This monoclonal antibody directed against CD56, NCAM, reacts with Molecule-1 (ICAM-1). The adhesion molecule ICAM-1 belongs to the 180 kD which has been identified as NCAM (Neural Cell Adhesion small cell lung carcinoma (SCLC) cells and with a minority of immunoglobulin super family, C2 subset; it is a ligand for the integrins Use: F, IP, WB Module). It is involved in intercellular adhesion and play a role in adenocarcinomas of the lung. The antibody is not reactive with squamous LFA-1 and MAC-1 and for CD43. Quantity: 50 µg outgrowth of neuritis and the development of the nervous system. cell carcinoma, large cell and the majority of adenocarci-nomas of the Several other isoforms have been identified which are expressed in a lung. In addition the antibody is not reactive with all kinds of Use: F, IF developmental and tissue specific pattern. Two major epitopes have lymphoma’s. The antibody reacts in immunoblot with a 145 kD protein Quantity: 100 µg CD55, Decay Accelerating Factor, DAF been defined, NKI-nbl-1 (MON 9012) reacts with epitope 1 and 123C3 present on neuro-endocrine and neural derivates. The antigen was Also available as BIOTIN conjugate (MON 1016B - 50 µg) (MON 9006) reacts with epitope 2. At the international Workshop on isolated from: small cell lung carcinoma derived cell line. SCLC antibodies 123C3 has been categorized as cluster 1 antibody Cat.no. MON 1155 (Monoclonal) (Stahel et al 1994). Most importantly, 123C3 can be used on routing Use: F, P CD54, Intracellular Adhesion Molecule-1, formalin fixed paraffin section after retrieval. All cells in small cell Quantity: 1 ml Clone MEM-118 carcinomas and carcinoids of the lung are strongly positive for 123C3. ICAM-1 This antibody reacts with the human CD55 antigen, decay accelerating A minority of cases of other major types of lung carcinoma are factor (DAF, GPI-linked, 70 kDa). In accord with its role in protecting sometimes positive as well: however this positivity is generally weak CD57, HNK1 Cat.no. MON 1132 (Monoclonal) tissues from complement mediated lyses, CD55 is broadly distributed and focal. Adenoid cystic carcinomas of bronchial glands are strongly among cells in contact with serum, including both haematopoietic and positive. Neuroblastomas and Wilms tumours are usually also staining Clone MEM 111 non-haematopoietic cells. An acquired disorder, PNH which leads to strongly positive. In non small lung cell carcinomas, 123C3 staining Cat.no. MON 1010 (Monoclonal) This antibody reacts with human broadly expressed adhesive molecule bouts of haemolysis, is at least partially caused by a deficiency of CD55 has been associated with more advanced stage and a decreased ICAM-1, the 90 kD glycoprotein Intracellular Adhesion Molecule-1 and CD59 as a result of defects in GPI anchor biosynthesis. survival after surgery (Kibbelaar et al. 1991). Positive staining with Clone 6-13-19-1 (CD54). The adhesion molecule ICAM-1 belongs to the Immunoglobulin other tumours, include medullary thyroid carcinomas and some ovarian The antibody reacts with a 110 kD antigen present on the peripheral family, it is a ligand for the intergrin LFA-1. Immunogen originated from Use: C, IP, WB tumours. NCAM (CD56) is a marker for natural killer: 123C3 can be blood lymphocyte subset (primarily large granular lymphocytes) Raji cell line. Quantity: 100 µg used to distinguish NK-cells from other haematopoietic cell populations. identified by the HNK-1 monoclonal antibody. It recognizes part of the Although expression of CD56 is uncommon among lymphomas, this Natural Killer cell population. CD57 is furthermore present on cells in Use: C, IP, WB, E feature has defined a distinctive and important category of lymphoma: neuroectodermal tissue. Quantity: 100 µg CD56 the putative natural killer (NK) cell lymphoma, which shows a Also available as FITC conjugate (MON 1132F - 0.5 ml) predilection for the upper aero digestive tract, skin, skeletal muscle, Use: F, IF, MC Also available as R-PE conjugate (MON 1132R - 0.5 ml) and other extra nodal sites and pursues an aggressive clinical course. Quantity: 1 ml (MON 1010-1) Use: C Cat.no. MON 2072 (Monoclonal) Furthermore, this antibody can be used to support diagnosis of 5 ml (MON 1010-5) Quantity: 0.5 ml lymphoma or to detect residual disease for cases of CD56 positive Clone MEM-188 T/NK -cell lymphoma in which the neoplastic lymphoid cells are small This antibody recognizes human CD56 antigen present on NK cells, and show minimal atypia, especially in small biopsies. CD57, HNK1 CD54, Intracellular Adhesion Molecule-1, 180 kDa. MEM-188 reacts with 180 kDa isoform characteristic for leukocytes. Use: F, IF, P ICAM-1 Quantity: 1 ml (MON 9006-1) Cat.no. MON 1012 (Monoclonal) Use: C, IP, WB 5 ml (MON 9006-5) Cat.no. MON 1133 (Monoclonal) Quantity: 100 µg Also available in purified format (MON 9006P - 100 µg) Clone NK-1 Also available as R-PE conjugate (MON 2072R - 0.5 ml) The antibody reacts with a 110 kD human lymphocyte cell surface Clone MEM 112 Also available as TRI-COLOR conjugate (MON 2072T - 0.5 ml) protein. It recognizes peripheral blood lymphocyte subset (primarily This antibody reacts with human broadly expressed adhesive molecule CD56, Neural Cell Adhesion Molecule, large granular lymphocytes). It recognizes parts of the Natural Killer cell. ICAM-1, the 90 kD glycoprotein Intracellular Adhesion Molecule-1 CD57 is furthermore present on cells in neuroectodermal tissue. Antigen (CD54). The adhesion molecule ICAM-1 belongs to the immunoglobulin NCAM origin: preparation of HSB-2 T-cell line. Antigen location: cell surface. family, it is a ligand for the integrin LFA-1. Immunogen originated from Raji cell line. Cat.no. MON 9012 (Monoclonal) Use: F, P Quantity: 1 ml Use: C, IP Clone NKI-nbl-1 Quantity: 100 µg The antibody recognizes a heterodimeric glycoprotein of 145, 185 kD which has been identified as NCAM (Neural Cell Adhesion Module). Two major epitopes have been defined, NKI-nbl-1 reacts with epitope 1 and 123C3 (MON 9006) reacts with epitope 2.

Use: F Quantity: 1 ml (MON 9012-1) 5 ml (MON 9012-5)

46 For research use only. Not for drug, diagnostic or other use. www.monosan.com 47 Cluster Differentiation Markers Cluster Differentiation Markers

CD58, LFA-3 CD59 CD61, Platelet GPIIIa, Vitronectin Receptor CD62E, E-Selectin, Biotinylated β-chain Cat.no. MON 1177 (Monoclonal) Cat.no. MON2086 (Monoclonal) NEW Cat.no. PS 017 (Polyclonal) Cat.no. MON 1147 (Monoclonal) Clone BRIC 5 Clone MEM-129 The antibody reacts with the Lectin Domain of E-select-in, CD62-E, LFA-3 is a membrane glycoprotein of 55-70 kDa which occurs either as The antibody reacts with leukocytes, platelets, erythrocytes. CD59 is Clone CRC54 formerly designated Endothelial Leukocyte Adhesion Molecule-1 a trans membrane moiety with a cytoplasmic domain or resident in the absent in patients with PNH and expressed on most types of cells. The anti CD61 antibody reacts with the platelet glycoprotein gpIIIa/VNR (ELAM-1). The antibody reacts with human endothelial cells activated

membrane. CD58 is a cell adhesion molecule of the Super Ig family, β-chain. This glycoprotein is the common β-chain (β3-Integrin) of the with TNF, IL-1 or endotoxin. The antibody was found to react also with and facilitates antigen recognition via CD2. The antigen is present on Use: C, FS cytoadhesins, gpIIbIIIa and VNR. The antibody reacts with platelets and cells transfected with the ELAM-1 gene. The antibody inhibits the erythrocytes, platelets, monocytes, bone marrow cells, a lymphocyte Quantity: 100 µg megakaryocytes. The antibody reacts preferentially with the activated/ adhesion of granulocytes both neutrophilic and eosinophilic. subset and epithelial and endothelial cells. occupied form of platelet GPIIb-IIIa (ligand-induced biding site or LIBS epitope) and dissociated form of GPIIIa. CRC54-epitope is located Use: F, IP, WB Use: C, WB, F CD60 within the N-terminal region of GPIIIa (first 100 amino acids). CRC54 Quantity: 50 µg Quantity: 1 ml does not react with platelets of patients with Glanzmann thrombastenia.

Cat.no. MON4054 (Monoclonal) NEW Use: C, F, IF CD62L CD59 Quantity: 1 ml Clone M-T6004 M-T6004 recognizes the human CD60 antigen, a carbohydrate of Cat.no. MON4039 (Monoclonal) NEW Cat.no. MON 1136 (Monoclonal) structure 9-0-acetyl GD3. Stains T-cell subsets and platelets. CD62E, ELAM-1, E-selectin Clone FMC46 Clone MEM-43 Use: C, F, IP FMC46 recognizes the human CD62L cell surface antigen, a 150kD The antibody is the prototypic antibody for the CD59 determinant, Quantity: 200 tests Cat.no. MON 6010 (Monoclonal) glycoprotein also known as L-selectin. CD62L is expressed by B and a PI-linked glycoprotein of 18-20 kD, which is broadly expressed on T lymphocytes, monocytes and granulocytes. most types of of cells. The antigen is the major complement-protecting Clone ENA1 molecule of human cells. It is expressed on all leukocytes, erythrocytes CD61, Platelet GPIIIa, Vitronectin Receptor ENA1 reacts with E-selectin CD62-E, previous designated as the Use: C, F, IP and most other cell types. Binding of this antibody inhibits the Endothelial Leukocyte Adhesion Molecule-1 (ELAM-1)[1-4]. The antibody Quantity: 100 µg complement-protecting activity of CD59. CD59 is one of the complement β-chain reacts with human endothelial cells activated with TNF, IL-1 or endotoxin. Also available as FITC conjugate (MON 4039F - 100 µg) regulatory membrane proteins that is missing in Paroxysmal Nocturnal The antibody was found to react also with cells transfected with the Also available as R-PE conjugate (MON 4039R - 100 tests) Haemoglobinuria (PNH). Anti CD59 reagents may therefore be useful Cat.no. MON 1051 (Monoclonal) ELAM-1 gene. The antibody inhibits the adhesion of granulocytes both in the differential diagnosis of PNH type II and PNH type I as reported neutrophilic and eosinophilic. (Sugita, Holguin). Clone BL-E6 CD62P, P-selectin, GMP 140, PADGEM The anti CD61 antibody recognizes the platelet glycoprotein gpIIIa/VNR Use: F

Use: C, IP β-chain. This glycoprotein is the common β-chain (β3-Integrin) of the Quantity: 1 ml Quantity: 100 µg cytoadhesins, gpIIIbIIIa and VNR. The antibody reacts with thrombocytes Also available as BIOTIN conjugate (MON 6010B - 0.5 ml) Cat.no. MON 1148 (Monoclonal) Also available as FITC conjugate (MON 1136F - 100 tests) and megakaryocytes. Clone CRC81 Use: F CD62E, ELAM-1, E-selectin, F(ab’)2 The monoclonal antibody is directed against the CD62-antigen, CD59 Quantity: 1 ml (an integral membrane sialoglycoprotein 140 kD) also designated, P-selectin, GMP-140 (granule membrane protein 140) or PADGEM, Cat.no. MON 6011 (Monoclonal) which is expressed in platelet alpha granules that are translocated to Cat.no. MON 1157 (Monoclonal) CD61, Platelet GPIIIa, Vitronectin Receptor the platelet surface upon activation with strong agonists. A similar Clone ENA2 protein, present in endothelial cell Weibel-Palade bodies, is transiently Clone MEM-43/5 β-chain, Conjugated ENA2 reacts with E-selectin CD62-E, previous designated as the expressed on the surface of activated endothelial cells and functions as This antibody reacts with leukocytes, platelets, and erythrocytes. CD59 Endothelial Leukocyte Adhesion Molecule-1 (ELAM-1)[1-4]. The antibody adhesion molecule for granulocytes. The antibody does not interfere with is absent in patients with PNH and expressed on most types of cells; Cat.no. MON 7051B/F (Monoclonal) reacts with human endothelial cells activated with TNF, IL-1 or endotoxin. the functional activity of CD62 as an adhesion receptor for neutrophils. 18-20 kDa CD59 function: complement-protecting membrane protein; The antibody was found to react also with cells transfected with the PI-linked protein. Holds similar specificity as H19. It recognizes an Clone CLB-thromb/1 E-selectin gene. The antibody inhibits the adhesion of granulocytes both Use: IF, WB

other epitope than MEM-43 does, and does not compete with MEM-43 This antibody recognizes the CD61 antigen. Also known as integrin β3, neutrophilic and eosinophilic. Quantity: 1 ml and most other known CD59 antibodies. the CD61 antigen is expressed on platelets, megakaryocytes, osteoclasts Also available as R-PE conjugate (MON 1148R - 0.5 ml) and endothelial cells. Use: F Use: C Use: C, WB Quantity: 1 ml Quantity: 0.5 ml Quantity: 100 µg Use: C Quantity: 0.5 ml Available as BIOTIN conjugate (MON 7051B - 0.5 ml) Available as FITC conjugate (MON 7051F - 0.5 ml)

48 www.monosan.com For research use only. Not for drug, diagnostic or other use. 49 Cluster Differentiation Markers Cluster Differentiation Markers

CD62P, P-selectin, GMP-140, PADGEM CD64, Monocyte CD66e, Granulocyte CD69, Early Activation Marker

Cat.no. MON 1137 (Monoclonal) Cat.no. MON 1169 (Monoclonal) Cat.no. MON 1139 (Monoclonal) Cat.no. MON 1054 (Monoclonal)

Clone CLB/thromb/6 Clone 10.1 Clone CLB-139 Clone BL-Ac/p26 The monoclonal antibody is directed against the CD62P-antigen, This antibody reacts with a single-chain glycoprotein of 75 kDa. Its main The monoclonal antibody is directed against the CD66e-antigen, a The antibody reacts with CD69, a 55-70 kD glycoprotein that under (an integral membrane sialoglycoprotein 140 kD) also designated cellular reactivity is with monocytes (up regulated by interferon gamma). 180-200 kD phosphoprotein, 90 kD PI-linked protein, which is expressed reducing conditions dissociates in 3 components (gp 26,28,34) being GMP-140 or PADGEM, which is expressed in platelet alpha granules that Other reactive cells in flow cytometry include interferon gamma on mature human granulocytes. After activation of the granulocutes, differentially glycosylated forms of a 21 kD protein. CD69 is an early are translocated to the platelet surface upon activation with strong stimulated granulocytes, myelomonocytic leukaemias and myeloid cell expression the antigen is strongly increased. It reacts with 100% of activation antigen also called Activation Inducer Molecule (AIM) that agonists. A similar protein, present in endothelial cell Weibel-Palade lines. Some histiocytes are recognized by this antibody in immunohisto- mature human peripheral granulocytes. The monoclonal antibody reacts is very early, before antigens such as CD25 and CD71 are expressed bodies, is transiently expressed on the surface of activated endothelial chemistry. Used antigen: human monocytes. weakly with malignant cells of patients with B-cell derived chronic strongly on T-cells after activation. CD69 is also expressed by activated cells and functions as adhesion molecule for granulocytes. Immunogen: lymphoid leukaemia (CLL). The monoclonal antibody does not react B-lymphocytes. human platelets. Use: C with normal human peripheral B-cells, T-cells, monocytes and platelets. Quantity: 2 ml In immunohistology the antibody reacts with a subpopulation of Use: C, F Use: IF tissue macrophages and the Carcino Embryonic Antigen (CEA) Quantity: 1 ml Quantity: 1 ml expressed on colon carcinoma and other carcinomas. Immunogen: CD64, Monocyte, R-PE conjugated human granulocytes. CD71, Transferrin Receptor CD63, Activated Platelets Use: IF Cat.no. MON 1195R (Monoclonal) Quantity: 1 ml Cat. no. MON 3086 (Monoclonal) NEW Cat.no. MON 1138 (Monoclonal) Clone 10.1 The antibody reacts with human CD64. CD67, Granulocyte Clone 3B8 2A1 Clone CLB-180 Human transferrin receptor 1 (TfR1), also designated CD71, is a The monoclonal antibody is directed against the CD63-antigen, a 53 kD Use: C homodimeric type II membrane glycoprotein of 90-95 kDa. This receptor glycoprotein, which is expressed in platelet lysosomes, that are located Quantity: 0.5 ml Cat.no. MON 1052 (Monoclonal) binds two molecules of the serum iron-transport protein transferrin to the platelet surface upon activation with strong agonists. The antigen (Tf) and is internalised into endosomes that are acidified, resulting in is also present in most peripheral blood cells (not in erythrocytes cells) Clone BL-B7 the release of iron from Tf. TfR1 is not expressed on resting leukocytes and in many tissues; both surface and cytoplasmic locations are reported. CD66acd The antibody reacts with CD66b, a 100 kD PI linked protein. The protein but is upregulated on all proliferating cells upon activation, reflecting Immunogen: human cytochrome B- enriched cells. is highly expressed on granulocytes, metamyelocytes and Chronic the iron dependence of proliferation. In tissues TfR1 is expressed on Myeloid Leukaemia cells in chronic phase. It is not know if this antibody most dividing cells and on brain capillary endothelium. Expression of Use: IF Cat.no. MON4047 (Monoclonal) NEW also activates granulocytes. TfR1 is down regulated as a result of iron overload. TfR1 shares 45% Quantity: 1 ml identity with TfR2. Clone YTH71.3 Use: C, F YTH71.3 recognizes human CD66acd. CD66acd is expressed by human Quantity: 1 ml Use: F, C, WB, IP, E CD63, Activated Platelets neutrophils and cells in some cases of colon carcinoma. Clone YTH71.3 Quantity: 100 µg reacts with transfectants containing BGPa, NCA and CGM1a which are CEA subfamily members(2). CD68, Monocytes, Macrophages Cat. no. MON 9000 (Monoclonal) NEW CDw70 Use: C, F Clone MEM-259 Quantity: 200 µg Cat.no. MON 1053 (Monoclonal) The antibody reacts with CD63 antigen (Melanoma 1 antigen), Cat.no. MON4065 (Monoclonal) NEW a glycoprotein of 40-60 kDa belonging to tetraspans family (TM4SF). Clone BL-A/D8 CD63 is intracellular lysosomal/endosomal/granule protein that is The antibody reacts with CD68, a 110 kD glycoprotein. It stains Clone BU69 translocated to cell surface upon activation of platelets, endothelial cells, macrophages in tissues. The CD68 antigen is primarily intracellular BU69 recognizes the human CDw70 cell surface antigen. CDw70 is and granulocytes (activation marker). CD63 is also expressed on present in monocytes and macrophages. expressed by a small number of activated T cells and by the majority monocytes/macrophages and endothelium. Its cellular function has not of activated B cells. been exactly elucidated. CD63 is thought to be associated with the Use: C, F early stages of melanoma tumor progression (in regulation of motility Quantity: 1 ml Use: C, F and adhesion of melanoma cells). Quantity: 200 µg

Use: C, IP, WB Quantity: 100 µg Also available as FITC conjugate (MON 9000F - 100 tests)

50 For research use only. Not for drug, diagnostic or other use. www.monosan.com 51 Cluster Differentiation Markers Cluster Differentiation Markers

CD71, Transferrin Receptor CD75, Sialoprotein CD79b CD82

Cat.no. MON 1009 (Monoclonal) Cat.no. MON 1166 (Monoclonal) Cat.no. MON 4093 (Monoclonal) NEW Cat.no. MON4053 (Monoclonal) NEW

Clone 66-IG10 Clone LN-1 Clone AT107-2 Clone B-L2 The antibody reacts with the transferrin receptor and is positive with This antibody is reactive with cell surface protein sensitive to neura- AT107-2 recognizes an intracellular region of human CD79b, a cell B-L2 recognizes the human CD82 antigen, a 50-53kD cell surface protein most proliferating cells. It reacts with a 190 kD molecule, which consists minidase, present on a subpopulation of mature B-cells, mainly surface antigen expressed by B lymphocytes. The CD79b molecule expressed by T-cells, NK cells, monocytes, granulocytes and platelets. of two subunits of 95 kD. The gene coding for the antigen is located on germinal centre B-cells. In non-lymphoid tissues, epithelial cells from (also known as Ig beta and B29) associates with CD79a to form a The molecule is a member of the tetra-spans family. the q-arm of chromosome 3. several organs and in the bone marrow red blood cell precursors are heterodimer that is covalently linked to surface immunoglobulin, stained. The LN-1 antibody is reactive on routinely processed paraffin forming the B-cell receptor (BCR) complex. Use: C, F Use: F, IF, IP embedded sections and is therefore an excellent marker for B-cell Quantity: 200 test Quantity: 1 ml (MON 1009-1) malignancies. Antigen location: cell surface. Antigen origin: lymphocytes Use: C, E, IP, WB 5 ml (MON 1009-5) and SU-DHL4 cell line. Quantity: 200 µg Also available as FITC conjugate (MON 4093F - 100 µg) CD83 Use: F, P CD71, Transferrin Receptor Quantity: 1 ml CD80 Cat.no. MON4060 (Monoclonal) NEW Cat.no. MON 1140 (Monoclonal) CDw76, B-cells Clone HB15e Cat.no. MON 2071 (Monoclonal) HB15e recognizes the human CD83 cell surface antigen, a 40-45kD Clone MEM-75 glycoprotein expressed by peripheral blood dendritic cells. Peripheral The antibody reacts with the human CD71 antigen, the transferrin- Cat.no. MON 1057 (Monoclonal) Clone MEM-233 lymphocytes can be induced to express very low levels of CD83 after receptor (a disulfide linked homodimer of 90 kD glycoprotein). The This antibody recognizes human CD80 antigen (B7, BB1) expressed on culture in agents such as Con A or PHA. In immunohistology CD83 is antigen is absent on resting B- and T-lymphocytes whereas it is expressed Clone BL-B8 activated B-cells, activated T-cells and macrophages, about 60 kDa shown to be expressed strongly by interfollicular interdigitating on activated lymphocytes. It is also present on a wide range of other The antibody reacts with a B-cell antigen that is associated with CDw76. (highly glycosylated molecule). reticulum cells and more weakly by cells within germinal centres. CD83 proliferating cells including neoplastic cells. It does not block binding The antibody precipitates besides the glycoproteins of 85/67 kD of is also expressed by Langerhan’s cells in the skin. The CD83 antigen is of transferrin to the receptor. CDw76 also 34/37/43 kD components. The binding of the antibody is Use: IP, WB a 186-amino-acid single-chain glycoprotein. This molecule is a member strongly inhibited by CDw76 reference antibodies. CDw76 is present on Quantity: 100 µg of the immunoglobulin superfamily and is composed of an extracellular Use: C, IP most mature, peripheral B-lymphocytes, and on a small subpopulation Also available as FITC conjugate (MON 2071F - 100 tests) V-type Ig-like single domain, a transmembrane region, and a short, Quantity: 100 µg of peripheral T-cells, monocytes and granulocytes. In tissue sections, 40-amino-acid cytoplasmic tail. CD83 antigen undergoes extensive Also available as FITC conjugate (MON 1140F - 100 tests) also standard formalin-fixed and paraffin-embedded tissue, the antibody post-translational glycosylation, since the determined Mr is twice the Also available as R-PE conjugate (MON 1140R - 100 tests) stains most B-lymphocytes of primary follicles, whereas in secondary CD81 predicted size of the core protein. (1). However, CD83+ cells have a follicles the staining is restricted to mantel zone lymphocytes. Some unique cell surface immuno-phenotype that does not correlate with macrophages may be labelled, especially in thymus cryostat sections. that of T cells, B cells, NK cells, or cells of the myelomonocytic lineage (2). CD72, pan B-cell Cat.no. MON 1076 (Monoclonal) CD83+ cells coexpress the highest levels of MHC class II molecules, Use: C, F, P when compared with other leucocyte lineages. They also co-express Quantity: 1 ml Clone 1.3.3.22 T cell markers (CD2, CD5), B cell markers (CD40, CD78), myeloid cell Cat.no. MON 1056 (Monoclonal) 1.3.3.22 reacts with human CD81 (TAPA-1), a 24 kD member of the TM4 markers (CD13, CD33, CD36), cytokine receptors as well as other cell super family of proteins with four trans membrane domains. Mab to surface molecules (2,3). Clone BL-A/A11 CDw78 CD81 have been shown to have anti-proliverative effects on different The antibody reacts with CD72, a glycoprotein composed of two chains, lymphoid cell lines, particularly those derived from large cell lymphomas. Use: C, F, P, IP 39 and 43 kD. The antigen is present on pre-B-cells in fatal liver and The mAb was clustered at the VI WLDA. Source: A BALB/c mouse was Quantity: 100 µg bone marrow, on all mature peripheral B-lymphocytes and is lost in Cat.no. MON 1074 (Monoclonal) immunized with a B-cell line derived from a Burkitt lymphoma. Spleno- Also available as BIOTIN conjugate (MON 4060B - 100 µg) terminal differentiation. It stains also B-lymphocytes in tissue sections. cytes were fused with mouse myeloma NS1-cells. Also available as FITC conjugate (MON 4060F - 100 µg) Clone 60-3G2 Also available as R-PE conjugate (MON 4060R - 100 tests) Use: C, F Cdw78 (also called Ba antigen, Leu21 or LO-panB-a) is present on some Use: C, IP Quantity: 1 ml immature and some mature B-cells. Its expression is greatly increased Quantity: 100 µg after B-cell activation in vitro (Kikutani et al. 1986). It is also found on tissue macrophages and on epithelial cells, but not on T-cells, NK-cells, monocytes, granulocytes and myeloid tissues (Dorken et al., 1989). One MoAb was shown to inhibit B-cell proliferation. Source: A BALB/c mouse was immunized and SP2/0 cells were used for fusion with splenocytes.

Use: F, C Quantity: 100 µg

52 www.monosan.com For research use only. Not for drug, diagnostic or other use. 53 Cluster Differentiation Markers Cluster Differentiation Markers

CD84 CD87, uPAR (D2 domain) CD89 CD91

Cat.no. MON 1077 (Monoclonal) Cat. no. MON 3095 (Monoclonal) NEW Cat.no. MON4068 (Monoclonal) NEW Cat.no. MON 4074 (Monoclonal) NEW

Clone 152-1D5 Clone IIIB11 Clone MIP8a Clone Alpha 2MR alpha-2 152-1D5 reacts with human CD84 (Mw 74 kD). CD84 is expressed on Monoclonal antibody IIIB11 recognizes the urokinase-type plasminogen MIP8a recognizes the human CD89 cell surface antigen, a 50-75kD cell This antibody recognizes human CD91, a type I membrane protein which mature B-cells and on B-cell lines but not on plasma cell lines. activator receptor (uPAR or CD87), a GPI anchored highly glycosylated, surface glycoprotein that is also known as the IgA receptor (Fc alpha R). is also known as the alpha 2 macroglobulin receptor (alpha 2MR). Immunohistochemical studies demonstrated that it strongly stains single-chain polypeptide. CD87, a 45- to 60-kD protein, consists of CD89 is expressed by peripheral blood monocytes and neutrophils. Alpha 2 MR alpha-2 recognizes an epitope in the extracellular region tissue macrophages. It is also expressed on platelets and at low levels three structurally homologous domains of approximately 90 amino acids, Clone MIP8a blocks binding of IgA to the Fc alpha R, and also inhibits of the 500kD alpha chain. on blood T-cells. Cellular expression does not significantly increase designated D1, D2, and D3, each containing a conserved arrangement neutrophil phagocytosis of IgA complexes. The preservative free format after activation 152-1D5 was clustered at the V WLDA. Source: A BALB/c of disulfide bonds and separated by short interdomain linker sequences. of this antibody is recommended for functional studies. Use: C, F, E, IP, WB mouse was immunized with MLR stimulated lymphocytes. Splenocytes uPAR exists in various molecular forms; heavily glycosylated, a cleaved Quantity: 100 µg were fused with mouse myeloma NS1-cells. form, a soluble form and spliced forms. CD87 is expressed by Use: C, E, IP, WB Also available as BIOTIN conjugate (MON 4074B - 100 µg) monocytes/macrophages, neutrophils, endothelial, smooth muscle cells Quantity: 100 µg Also available as FITC conjugate (MON 4074F - 100 µg) Use: C, IP and epithelial cells. CD87 is over expressed upon exposure to Also available as FITC conjugate (MON 4068F - 100 µg) Also available as R-PE conjugate (MON 4074R - 100 tests) Quantity: 100 µg inflammatory mediators. The protein is also highly expressed by tumor Also available as R-PE conjugate (MON 4068R - 100 tests) cells and contributes to their invasiveness and metastatic potential. CD87 is a multiligand receptor that operates as a key element in CD94 CD84 pathophysiological processes involving cell migration and tissue CD90 remodelling during inflammation and cancer metastasis. CD87 binds with high affinity the Ser-proteinase urokinase-type plasminogen Cat.no. MON 4096(Monoclonal) NEW Cat.no. MON 1005 (Monoclonal) activator (uPA) which, upon binding catalyzes the conversion of Cat.no. MON 2003 (Monoclonal) plasminogen into plasmin which activates various matrix metallo- Clone DX22 Clone 153-4D9 proteinase leading to a high potential for pericellular proteolysis. CD87 Clone AF-9 DX22 is specific for human CD94, also known as KLRD1 (Killer cell 153-4D9 reacts with human CD84 (Mw 74 kD). CD84 is expressed on functions also as an adhesion molecule by binding vitronectin, thus CD90 is also known as Thy-1 antigen and a member of the immuno- lectin-like receptor, subfamily D, member 1). CD94 is expressed on natural mature B-cells and on B-cell lines but not on plasma cell lines. contributing to leukocytic adherence. High-affinity binding of CD87 to globulin super family. It may contribute to inhibition of proliferation/ killer (NK) cells and a subset of T lymphocytes. Immunohistochemical studies demonstrated that it strongly stains both uPA and vitronectin requires the presence of domain 1 (D1). differentiation of hematopoietic stem cells and neuron memory CD94 is found to associate with NKG2 to form a heterodimer which is tissue macrophages. It is also expressed on platelets and at low levels Yet, CD87 participates in cell adherence and migration also through formation in the CNS. It consists of a single Ig domain (112 amino acids; involved in the inhibition of cell mediated cytotoxicity against cells on blood T-cells. Cellular expression does not significantly increase two other routes, a physical association and functional interaction with 25-35 kD) inserted into the cell membrane via a GPI anchor. Expressed bearing appropriate MHC class I allotypes. after activation 153-4D9 was clustered at the V WLDA. Source: A BALB/c various integrins and via an intrinsic chemotactic activity associated to by hematopoietic stem cells and neurons in all species studied. Its mouse was immunized with MLR stimulated lymphocytes. Splenocytes the D1-D2 linker sequence. Monoclonal antibody IIIB11 recognizes D2 highly expressed in connective tissue and various fibroblast and stromal Use: C were fused with mouse myeloma NS1-cells. of the urokinase-type plasminogen activator receptor (CD87). The cell lines, expressed on all thymocytes and peripheral T-cells in mice, Quantity: 100 µg antibody recognizes amino acid sequences 125-132 of the D2 domain. but in humans expressed only on small % fetal thymocytes, 10-40% of Also available as BIOTIN conjugate (MON 4096B - 100 µg) Use: C, IP CD34+ cells in bone marrow, and <1% of CD3+CD4+ lymphocytes in Also available as FITC conjugate (MON 4096F - 100 µg) Quantity: 100 µg Use: WB, C, F, P peripheral circulation. It is also expressed by human lymph node HEV Also available as R-PE conjugate (MON 4096R - 100 tests) Quantity: 100 µg endothelium but not other endothelia. Lastly, it is expressed by a limited number of lymphoblastoid and leukemic cell lines. CD86 CD95 (FAS antigen) CD88 Use: F, C, E Quantity: 100 µg Cat.no. MON4041 (Monoclonal) NEW Cat.no. MON 2010 (Monoclonal) Cat.no. MON 4084 (Monoclonal) NEW Clone BU63 CD90, rat leukocyte markers (Thy-1) Clone B-R18 BU63 recognizes CD86, a 70kD cell surface protein expressed by Clone P12/1 The mAb B-R18 specifically recognizes CD95. The CD95 molecule is a activated B cells and monocytes. CD86 is also known as B7-2 and is This antibody recognizes the C5a receptor (C5aR) CD88, which is cell surface glycoprotein with a MW of 40-45 kDa and contains 8 kDa the second ligand for CD28 and CTLA-4. It may have an important role predominantly expressed on cells of the myeloid lineage. Clone P12/1 Cat.no. MON 2030 (Monoclonal) of N-glycosydic-linked polysaccharide. CD95 antigen is preferentially in co-stimulation of T cells in the primary immune response. was raised against a synthetic peptide comprising the N-terminal expressed by the CD45RAlow CD45ROhigh subset of memory extracellular domain of the C5aR (met1-Asn31). Clone P12/1 does not Clone 3-3B5 T-lymphocytes. Source: BALB/c mice (Iffa Credo) were immunized with Use: C, F, IP inhibit the binding of C5a to its receptor. This monoclonal antibody recognizes Thy-1. It is reactive with all common purified recombinant Fas antigen and fused with X63/Ag.8653 mouse Quantity: 200 µg inbred strains. myeloma cells. Also available as FITC conjugate (MON 4041F - 100 µg) Use: C, F, WB Also available as R-PE conjugate (MON 4041R - 100 tests) Quantity: 200 µg Use: C, F Use: C Also available as FITC conjugate (MON 4084F - 100 µg) Quantity: 100 µg Quantity: 100 µg

54 www.monosan.com For research use only. Not for drug, diagnostic or other use. 55 Cluster Differentiation Markers Cluster Differentiation Markers

CD95 (FAS/APO-1) CD98 CD101 CD104

Cat.no. MON 2038 (Monoclonal) Cat.no. MON 1070 (Monoclonal) Cat.no. MON 4094 (Monoclonal) NEW Cat.no. MON4057 (Monoclonal)

Clone ICO-160 Clone MEM-108 Clone BB27 Clone 450-9D ICO-160 detects CD95 selectively expressed by activated lymphocytes The antibody reacts the CD98 antigen. The specificity has been BB27 recognizes human CD101. CD101 is a 140kD homodimeric cell 450-9D recognizes the human beta4 integrin (also known as CD104), and by a subset of monocytes in peripheral blood and induces apoptosis determined by flow cytometry with PBL and several cell lines and surface glycoprotein expressed primarily by monocytes, granulocytes, a 205kD glycoprotein which associates with the alpha6 integrin to of activated T- and B-cells, NK-cells an thymocytes. CD95 is a trans reactivity with CD97 transfectants. dendritic cells and activated T lymphocytes. form the alpha6/beta4 complex. CD104 is expressed on epithelial cells, membrane glycoprotein of 40 to 50 kD with N-glycosidic-linked Studies suggest that CD101 plays a major role in the activation of T cells Schwann cells and various tumour cell lines. 450-9D recognizes an polysaccharides. Source: A BALB/c mouse was immunized with activated Use: C by skin dendritic cells. Clone BB27 has been reported to inhibit extracellular epitope on the CD104 molecule. PBL and the isolated splenocytes were fused with NS1- cells. Quantity: 100 µg allogeneic T-cell responses. Use: C, F, IP, WB Use: F, C Use: C, F, IP Quantity: 200 µg Quantity: 100 µg CD99 Quantity: 200 µg Also available as FITC conjugate (MON 4057F - 100 µg) Also available as FITC conjugate (MON 4094F - 100 µg) Also available as R-PE conjugate (MON 4094R - 100 tests) CD97 Cat.no. MON 2015 (Monoclonal) CD105, Endoglin Clone H036-1.1 CD102 Cat.no. MON1197 (Monoclonal) HO36-1.1 reacts with CD99 or MIC2. Human thymocytes, and some Cat. no. MON 3091 (Monoclonal) NEW T-ALL isolates and cell lines are positive. Source: A (CBAxBALB/c)F1 Clone MEM-180 mouse was immunized with purified E-rosette forming cells from Cat.no. MON4044 (Monoclonal) NEW Clone E9 Human CD97 antigen, expressed mainly on myeloid cells and activated Human PBL. The monoclonal antibody E9 reacts with Endoglin a 190 kDa lymphocytes; 74-89 kDa. Clone B-T1 homodimeric transmembrane glycoprotein composed of disulfide-linked Use: C, WB B-T1 recognizes the human CD102 cell surface molecule, a 55-65kD subunits. The external domain binds TGF-beta1 and -beta3 isoforms Use: C, IP Quantity: 100 µg glycoprotein also known as ICAM-2. MCA1140 inhibits cell adhesion with high affinity. Two different isoforms (L and S) of CD105 with Quantity: 100 µg and T cell activation and also recognizes soluble ICAM-2. capacity to bind TGF-beta have been characterized which differ in the amino acid composition of their cytoplasmic tails. Mutations in the CD99 Use: C, IP, WB gene encoding endoglin have been linked to the human disease CD98 Quantity: 200 µg hereditary hemorrhagic telangiectasia type 1 (HHT1) a vascular disorder characterized by localized vascular dysplasia and a tendency towards Cat.no. MON 1158 (Monoclonal) arteriovenous malformations. Mice expressing a single CD105 allele Cat.no. MON 2013 (Monoclonal) CD103 develop external signs of disease similar to human HHT1, supporting Clone MEM-131 the haploinsufficiency model for HHT1. Mice lacking endoglin die from Clone IPO-T10 This antibody has a broad reactivity, including thymocytes and blood defective angiogenesis characterized by failure of vascular smooth IPO-T10 recognize CD98. The CD98 molecule is a heterodimer made of T-lymphocytes. CD99 function: MIC2 molecule, E2 adhesion molecule, Cat.no. MON4055 (Monoclonal) NEW muscle investment of embryonic blood vessels, suggesting a defect in a disulfide-linked glycosylated heavy chain of approximately 80-90 involved in E-rosette formation. Gene location: Xp22.32-pter Yp11.2-pter. vascular smooth muscle cell development. kDa and a non-glycosylated light chain. The heavy chain is a type II Clone LF61 Microvessel density (MVD) has been reported to be an independent integral membrane protein containing a 50-81 amino acid NH2-terminal Use: C, IP, WB LF61 recognizes the human CD103 cell surface antigen, a glycoprotein prognostic indicator of outcome in a variety of human malignancies, cytoplasmic domain, a single trans membrane sequence, and a large Quantity: 100 µg expressed by approximately 1% of peripheral blood lymphocytes, with increased MVD correlating with shorter overall and relapse-free extra cellular domain. CD98 is detected on monocytes and activated activated T lymphocytes and by hairy cell leukaemia cells. The antigen survival rates. The MVD counts using anti-CD105 antibody significantly cells. Source: After repeated immunization of BALB/c mice, IPO-T10 was is also expressed by intraepithelial lymphocytes. It has recently been correlated with overall and disease-free survival. Anti-CD105 monoclonal generated by fusion of the mouse splenocytes with the mouse P3-X63- CD100 shown to be identical to the alpha E integrin. antibody E9 and anti-CD34 monoclonal antibody have been successfully Ag8.653 mouse myeloma cell line. used to quantify MVD in human breast carcinoma Use: C, F, IP Use: F, C Cat.no. MON 2017 (Monoclonal) Quantity: 100 µg Use: F, E, IP, C, F Quantity: 100 µg Also available as FITC conjugate (MON 4055F - 100 µg) Quantity: 100 µg Clone 133.1G6 Also available as R-PE conjugate (MON 4055R - 100 tests) 133.1G6 reacts with human CD100, a novel 150 kD homodimer cell- surface antigen that is expressed on resting and PHA-stimulated T-cells. The protein is weakly expressed on NK-cells, EBV transformed B-cells, monocytes and tumour T-cell lines. The mAb was clustered at the IV WLDA (workshop no. A022). Source: A BALB/c mouse was immunized with PHA stimulated PBL. Splenocytes were fused with mouse myeloma NS1-cells.

Use: C, IP Quantity: 100 µg

56 For research use only. Not for drug, diagnostic or other use. www.monosan.com 57 Cluster Differentiation Markers Cluster Differentiation Markers

CD105 CD108 CD117, C-KIT CD120a, TNF-R I (p55/p60), Mouse

Cat.no. MON 2070 (Monoclonal) Cat.no. MON1163 (Monoclonal) Cat.no. MON 1099 (Monoclonal) Cat.no. MON 9064 (Monoclonal)

Clone MEM-226 Clone MEM-150 Clone T595 Clone HM104 This antibody recognizes human broadly expressed CD98 antigen Human GPI-anchored CD108 antigen, present on some T-cell lines, Prokaryotic recombinant protein corresponding to the three N-terminal The antibody reacts with the extra cellular part of the mouse TNF- (also known as 4F2), 80+40 kDa. activated lymphocytes and erythrocytes, (identical to the JMH blood C2-like extra cellular domains. The c-kit proto-oncogene encodes a Receptor p55 and with the soluble receptor. TNF-Receptor p55 is present group antigen); 80 kDa. trans membrane receptor with tyrosine kinase activity, c-kit (CD117) is on most cell types and is considered to play a prominent role in cell Use: WB, C closely related to the platelet-derived growth factor receptor family. stimulation by TNF-α. Induction of cytotoxicity and other most functions Quantity: 100 µg Use: C, IP, WB It is expressed in normal breast epithelium, melanocytes, mast cells, glial are mediated largely via TNF-Receptor p55. Also available as FITC conjugate (MON 2070F - 100 tests) Quantity: 100 µg cells. In neoplasms the antigen is expressed in SLCC, breast carcinomas, glioblastomas, melanomas and acute/chronic myelogenous leukaemias Use: E, F, IP, C during myeloid blast crises. Quantity: 100 µg CD105, Endoglin CD112 Use: P Quantity: 1 ml CD120a, TNF-R I (p55/p60) Cat.no. MON 6016 (Monoclonal) Cat.no. MON4071 (Monoclonal) NEW

Clone PN-E2 Clone R2.525 CD119 Cat.no. MON 9062 (Monoclonal) The antibody reacts with the human endoglin molecule, a membrane- R2.525 recognizes CD112 Poliovirus Receptor Related 2 (PRR2), a new associated homodimer of 180 kD. Endoglin is a TGFβ-binding protein surface molecule, which has been reported to be homologous to the Clone MR1-2 that is most abundantly expressed on endothelial cells in tissues with poliovirus receptor (CD155). Studies demonstrate that PRR2 expression Cat.no. MON4056 (Monoclonal) NEW The antibody reacts with the extra-cellular part of the TNF-R I. It also inflammatory or inflammatory-like characteristics, such as the skin lesions is restricted to the myelo-monocytic and megakaryocytic lineages. reacts with the soluble receptor. TNF-R I is present on most cell types in psoriasis. Proliferating endothelial cells in granulation tissue and in PRR2 expression has also been detected on haematopoeitic progenitors Clone BB1E2 and is considered to play a prominent role in cell stimulation by TNF- tumours (e.g. melanoma of the skin) also exhibit enhanced expression expressing CD34. In Western Blotting only one protein band is stained corresponding to a alpha: Induction of cytotoxicity and other functions are mediated largely of endoglin. In addition, a marked staining of syncytiotrophoblasts in MW of 43,000, which is equal to the MW for the glycosylated extra- via TNF-R I. The antibody cross reacts with rhesus and cynomolgus placenta is observed. In vitro the antibody reacts strongly with cultured Use: C, F, IP cellular part of the human extra-cellular interferon-gamma receptor natural TNF-R I. endothelial cells derived from the umbilical vein and with micro vascular Quantity: 200 µg (fused to c-myc), and a MW of 27,000 corresponding to the non- endothelial cells from the foreskin, as detected by immunofluorescence glycosylated part (fused to c-myc). The antibody does not neutralise Use: C, F, E, WB, IP and ELISA techniques. The antibody PN-E2 shows a weak staining with biological activity of interferon gamma. Quantity: 100 µg fibroblasts, monocytes and macrophages. CD114 Also available as BIOTIN conjugate (MON 9062B - 0.5 ml) Use: C, F, E, WB Use: F Quantity: 200 µg Quantity: 1 ml (MON 6016-1) Cat.no. MON4058 (Monoclonal) NEW Also available as FITC conjugate (MON 4056F - 100 µg) CD120a, TNF-R I (p55/p60) 5 ml (MON 6016-5) Clone LMM775 LMM775 recognizes the human G-CSF receptor. This antibody does not CD120a, TNF-R I (p55/p60) Cat.no. PS 053 (Polyclonal) CD106, VCAM-1, vascular-cell adhesion block binding of G-CSF to its receptor. The antibody reacts with the extra-cellular part of the TNF-R I and with molecule-1 Use: C Cat.no. MON 9058 (Monoclonal) the soluble receptor. TNF-R I is present on most cell types and is Quantity: 250 µg considered to play a prominent role in cell stimulation by TNF-alpha. Cat.no. MON 6017 (Monoclonal) Also available as R-PE conjugate (MON 4058R - 100 tests) Clone H398 Induction of cytotoxicity and other functions are mediated largely via The antibody reacts with the extra-cellular part of the TNF-RI. It also TNF-R I. Clone 1G11B1 reacts with the soluble receptor. TNF-RI is present on most cell types The antibody reacts with VCAM-1, the vascular cell adhesion molecule CD116 R-PE, Conjugated and is considered to play a prominent role in cell stimulation by TNF-α. Use: E, WB, IP (1), which is a member of the immunoglobulin super family. The antigen Induction of cytotoxicity and other functions are mediated largely via Quantity: 100 µg is induced on human endothelial cells by TNF, IL-1, IFN-α or endotoxin. TNF-RI. The ligand for VCAM-1 is VLA-4 (CD49d/CD29) an integrin that is Cat.no. MON4048R (Monoclonal) NEW constitutively expressed by thymocytes, lymphocytes and monocytes. Use: C, F, IP, WB Clone K12B7 Quantity: 100 µg Use: F, E K12B7 recognizes the human CD116 cell surface antigen, also known Also available as BIOTIN conjugate (MON 9058B - 50 µg) Quantity: 100 µg as the alpha chain of the GM-CSF receptor. CD116 is expressed by Also available as BIOTIN conjugate (MON 6017B - 50 µg) neutrophils, monocytes and eosinophils. K12B7 binding to the GM-CSF receptor inhibits binding of GM-CSF.

Use: C Quantity: 100 tests

58 www.monosan.com For research use only. Not for drug, diagnostic or other use. 59 Cluster Differentiation Markers Cluster Differentiation Markers

CD120a, TNF-R I (p55/p60), Mouse CD120b, TNF-R II (p75/p80) CDw121a, Interleukin-1 Receptor Type 1 (p80) CDw121a, Interleukin-1 Receptor Type 1 (p80)

Cat.no. PS 055 (Polyclonal) Cat.no. PS 054 (Polyclonal) Cat.no. MON 5077 (Monoclonal) Cat.no. MON 5076 (Monoclonal)

The antibody reacts with the extra-cellular part of the mouse TNF-R I The antibody reacts with the extra-cellular part of the TNF-R II and Clone D1f3 Clone Reg20 and with the soluble receptor. TNF-R I is present on most cell types and with the soluble receptor. TNF-R II is present on most cell types and is This monoclonal antibody binds to soluble mouse interleukin-1 receptor This monoclonal antibody binds to soluble mouse interleukin-1 receptor is considered to play a prominent role in cell stimulation by TNF-alpha. considered to play a role in cell stimulation by TNF-alpha. TNF-R II type I. The IL-1 system includes two agonists (IL-1a and IL-1b), converting type I. The IL-1 system includes two agonists (IL-1α and IL-1β), converting Induction of cytotoxicity and other functions are mediated largely via molecule is shown to be responsible for stimulation of activated enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 TNF-R I. A minor cross-reactivity with mouse TNF-alpha is T-lymphocytes by TNF-alpha. receptor accessory protein. Interleukin-1 signal is transduced through receptor accessory protein. Interleukin-1 signal is transduced through detectable. the type I receptor. the type I receptor. Use: E, WB, IP, C Use: E, WB, IP, C Quantity: 100 µg Use: E, WB, IP, inh. (for in vitro use the monoclonal MON 5075 Use: E, WB, IP Quantity: 100 µg is recommended) Quantity: 100 µg Quantity: 100 µg CD120b, TNF-R II (p75/p80), Mouse CD120b, TNF-R II (p75/p80) CDw121b, Interleukin-1 RII CDw121a, Interleukin-1 Receptor Type 1 (p80) Cat.no. PS 056 (Polyclonal) Cat.no. MON 9063 (Monoclonal) Cat.no. MON 5084 (Monoclonal) The antibody reacts with the extra-cellular part of the mouse TNF-R II Cat.no. MON 5078 (Monoclonal) Clone MR2-1 and with the soluble receptor. TNF-R II is present on most cell types and Clone 6G5 The antibody reacts with the extra-cellular part of the TNF-RII. It also is considered to play a role in cell stimulation by TNF-alpha. TNF-R II Clone D14e3 The antibody reacts specifically with human IL-1 RII. The IL-1 system reacts with the soluble receptor. TNF-RII is present on most cell types and molecule is shown to be responsible for stimulation of activated This monoclonal antibody binds to soluble mouse interleukin-1 receptor includes two agonists (IL-1α and IL-1β), converting enzymes, antagonists, is considered to play a prominent role in cell stimulation by TNF-alpha. T-lymphocytes by TNF-alpha. Minimal cross reactivity with mouse type I. The IL-1 system includes two agonists (IL-1α and IL-1β), converting two receptors (IL-1 RI and IL-1 RII) and the IL-1 receptor accessory TNF-RII molecule is shown to be responsible for stimulation of activated TNF-alpha. enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 protein. The IL-1 RII is part of the antagonistic IL-1 mechanism. It is T-lymphocytes by TNF-alpha. The antibody cross reacts with rhesus and receptor accessory protein. Interleukin-1 signal is transduced through also known as decoy receptor and is a non-signalling molecule which cynomolgus natural TNF-RII. Use: E, WB, IP the type I receptor. functions by capturing IL-1 and preventing it from interacting with the Quantity: 100 µg signalling IL-1 RI. The decoy IL-1 RII can after binding to IL-1 also recruit Use: C, F, E, WB, IP Use: WB the IL-1 receptor accessory protein and thus inhibit by co receptor Quantity: 100 µg Quantity: 100 µg competition. Further a soluble form of IL-1 RII exists which is shed, a Also available as BIOTIN conjugate (MON 9063B - 0.5 ml) CD120b, TNF-R II (p75/p80) process in which matrix metalloproteases have been found to play a role, by various cells including monocytes, polymorph nuclear cells, B-cells CDw121a, Interleukin-1 Receptor Type 1 (p80) and fibroblasts. CD120b, TNF-R II (p75/p80), Mouse Cat.no. MON 9059 (Monoclonal) Use: E Clone 80M2 Cat.no. MON 5075 (Monoclonal) Quantity: 100 µg Cat.no. MON 9065 (Monoclonal) The antibody reacts with the extra-cellular part of the TNF-RII. It also reacts with the soluble receptor. TNF-RII is present on most cell types Clone Reg21 Clone HM102 and is considered to be an important co-stimulator together with TNF-RI This monoclonal antibody binds to soluble mouse interleukin-1 receptor CDw121b, Interleukin-1 RII The antibody reacts with the extra-cellular part of the mouse TNF-RII in cellular responses to TNF-alpha, in particular the transmembrane form type I. The IL-1 system includes two agonists (IL-1α and IL-1β), converting and with the soluble receptor. TNF-RII is present on most cell types and of TNF-alpha. A dominant role of TNF-RII has been shown in thymocyte enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 is considered to play a prominent role in cell stimulation by TNF-alpha. activation by TNF-alpha. receptor accessory protein. Interleukin-1 signal is transduced through Cat.no. MON 5079 (Monoclonal) The TNF-RII molecule is shown to be responsible for stimulation of the type I receptor. activated T-lymphocytes by TNF-alpha. Use: C, F, IP, WB, E Clone 8.5 Quantity: 100 µg Use: E, WB, IP, inh. (for in vivo use the monoclonal MON 5077 This monoclonal antibody reacts specifically with human IL-1 RII. The Use: E, F, IP, C Also available as BIOTIN conjugate (MON 9059B - 50 µg) is recommended) IL-1 system includes two agonists (IL-1α and IL-1β), converting enzymes, Quantity: 100 µg Quantity: 100 µg antagonistic, two receptors (IL-1 R1 and IL-1 RII) and the IL-1 receptor accessory protein. The IL-1 RII is part of the antagonistic IL-1 mechanism. It is also known as decoy receptor and is a non-signalling molecule which functions by capturing IL-1 and preventing it from interacting with the signalling IL-1 RI. The decoy IL-1 RII can after binding to IL-1 also recruit the IL-1 receptor accessory protein and thus inhibit by co receptor competition. Further a soluble form of IL-1 RII exists which is shed, a process in which matrix metalloproteases have been found to play a role, by various cells including monocytes, polymorph nuclear cells, B-cells and fibroblasts.

Use: WB, C, E Quantity: 100 µg

60 For research use only. Not for drug, diagnostic or other use. www.monosan.com 61 Cluster Differentiation Markers Cluster Differentiation Markers

CDw121b, Interleukin-1 RII CD130 CD138 CD143, conjugated

Cat.no. PS 029 (Polyclonal) Cat.no. MON4043 (Monoclonal) NEW Cat.no. MON 5097 (Monoclonal) NEW Cat.no. MON 4083B/F/R (Monoclonal) NEW

This polyclonal antibody reacts specifically with human IL-1 RII. The IL-1 Clone B-T2 Clone B-B4 Clone i2H5 system includes two agonists (IL-1α and IL-1β), converting enzymes, B-T2 specifically recognizes soluble and membrane bound gp130 B-B4 recognizes a heparan sulfate rich membrane glycoprotein of 200kD i2H5 recognizes human CD143, also known as angiotensin - converting antagonistic, two receptors (IL-1 R1 and IL-1 RII) and the IL-1 receptor receptor. known as Syndecan-1 (CD138). Syndecan functions as a receptor for enzyme (ACE). CD143 exists in two forms, a 170KD somatic form and accessory protein. The IL-1 RII is part of the antagonistic IL-1 mechanism. This antibody has been reported to block the activity of IL-6. collagen, fibronectin and thrombospondin. a 90KD germinal form. The somatic form is expressed by endothelial It is also known as decoy receptor and is a non-signalling molecule B-B4 stains all plasma cells and plasma cell lines. No staining of other cells (especially those of arterioles and lung capillaries), epithelial cells which functions by capturing IL-1 and preventing it from interacting Use: C, E leucocytes is seen in peripheral blood or bone marrow, although some (especially in proximal renal tubules and in the small intestine), by some with the signalling IL-1 RI. The decoy IL-1 RII can after binding to IL-1 Quantity: 100 µg ALL cell lines express the antigen weakly. Some staining of epithelial neuronal cells and variably on some macrophages and T lymphocytes. also recruit the IL-1 receptor accessory protein and thus inhibit by co and endothelial cells may be seen in immunohistology. The germinal form is expressed by spermatozoa. receptor competition. Further a soluble form of IL-1 RII exists which is shed, a process in which matrix metalloproteases have been found to CD135 Use: C, F, P, IP, WB Use: C play a role, by various cells including monocytes, polymorph nuclear cells, Quantity: 200 tests Quantity: 100 µg B-cells and fibroblasts. Available as BIOTIN conjugate (MON 4083B - 100 µg) Cat.no. MON4069 (Monoclonal) NEW Available as FITC conjugate (MON 4083F - 100 µg) Use: E CD139 Available as R-PE conjugate (MON 4083R - 100 tests) Quantity: 100 µg Clone BV10A4H2 BV10A4H2 recognizes the human CD135 cell surface antigen, also known as FLT3. This antigen is a glycoprotein of 155-160kD, which may Cat.no. MON4066 (Monoclonal) NEW CD146 CD122 also be expressed as a more ‘immature’ 130kD molecule. CD135 is expressed by monocytes, and also by a subset of CD34+ve bone marrow Clone BU30 cells, which appear to be myeloid progenitor cells. BU30 recognizes the human CD139 antigen. CD139 is expressed by Cat.no. MON 4086 (Monoclonal) NEW Cat.no. MON4072 (Monoclonal) NEW germinal centre B cells, follicular dendritic cells and the follicular mantle. Use: C, IP Smooth muscle of some blood vessels is also stained in some cases. Clone OJ79c Clone MIK-Beta 1 Quantity: 100 µg OJ79c recognizes the 118kD cell surface glycoprotein CD146, also known MIK-Beta 1 recognizes the human CD122 cell surface glycoprotein, a Also available as BIOTIN conjugate (MON 4069B - 100 µg) Use: F as MUC18, Mel-CAM and S-endo. CD146 is a member of the immuno- 70-75kD molecule also known as the IL-2 receptor Beta-chain. CD122 is Also available as FITC conjugate (MON 4069F - 100 µg) Quantity: 200 µg globulin superfamily, expressed by all endothelial cells and by expressed by a subset of lymphocytes and NK cells, and is an Also available as R-PE conjugate (MON 4069R - 100 tests) melanoma cells. important component of both the IL-2 and IL-15 receptor complexes. CD146 appears to act as an adhesion molecule. Expression in melanoma CD141 may be linked to tumour progression. Use: C CDw137 Quantity: 200 µg Use: C, F, E Also available as FITC conjugate (MON 4072F - 100 tests) Cat.no. MON 5096 (Monoclonal) NEW Quantity: 200 µg Cat.no. MON4062 (Monoclonal) NEW Also available as FITC conjugate (MON 4086F - 100 µg) Clone QBEND/40 CDw128, Interleukin-8R1 (CXCR1) Clone 4B4-1 The antibody recognizes all human endothelium tested. Preliminary 4B4-1 recognizes the human CDw137 cell surface antigen, also known evidence suggests that this antibody recognizes Thrombomodulin, but CD147 as 4-1BB. CDw137 is not expressed by resting peripheral blood T cells, does not inhibit Thrombomodulin dependent activation of protein C. Cat.no. MON 5049 (Monoclonal) but is expressed following activation by PHA or anti-CD3. Use: C, F, P Cat.no. MON 2034 (Monoclonal) Clone NVR1 Use: C Quantity: 100 tests The antibody reacts with Human IL-8 Receptor type I better known as Quantity: 200 µg Clone CB43 CXCR1. There are two receptors for IL-8: CXCR1 (IL-8RA/R1) and CXCR2 Also available as FITC conjugate (MON 4062F - 100 µg) CB43 recognizes the antigen CD147. This antigen is a single chain type (IL-8RB/R2). One receptor, CXCR2, has high affinity for IL-8 and all Also available as R-PE conjugate (MON 4062R - 100 tests) I trans membrane with a MW of 50-60 kDa. It has been described as a other CXC chemokines that attract neutrophils, while the other, CXCR1 tumour-cell-derived soluble factor that stimulates expression of has high affinity for IL-8 only. IL-8 receptors are also found on monocytes, collagenases by fibroblasts. Source: A BALB/c mouse was immunized. basophils and eosinophils, but the responses of these cell to IL-8 are After three weeks the mouse was boosted and the splenocytes fused much weaker than those of neutrophils. IL-8 induces neutrophil with myeloma cell line NSO. A positive hybridoma obtained after three chemotaxis predominantly via Type 1 IL-8 Receptor CXCR1. limiting dilutions served as source for the hybridoma.

Use: Inh, C Use: F Quantity: 100 µg Quantity: 100 µg Also available as BIOTIN conjugate (MON 5049B - 50 µg)

62 For research use only. Not for drug, diagnostic or other use. www.monosan.com 63 Cluster Differentiation Markers Cluster Differentiation Markers

CD147, Neurothelin, Basigin, M6 CD154 CD161 CD165

Cat.no. MON 1071 (Monoclonal) Cat.no. MON4059 (Monoclonal) NEW Cat. no. MON 9052 (Monoclonal) NEW Cat.no. MON4070 (Monoclonal) NEW

Clone MEM-M6/1 Clone TRAP1.3.6 Clone LT161 Clone AD2-13H12 The antibody reacts the CD147 antigen. The antibody recognizes the TRAP1.3.6 recognizes the human CD154 cell surface antigen, a 32kD The antibody recognizes the CD161 antigen. AD2-13H12 recognizes the 36kD membrane glycoprotein CD165 (also N-terminal Ig-domain of the receptor. The specificity has been determined glycoprotein also known as CD40 ligand. CD154 is expressed on known as AD2). CD165 is an adhesion molecule which is expressed by by flow cytometry with PBL and several cell lines, reactivity with CD98 activated T lymphocytes, predominantly CD4+ve and also on some Use: C a subset of peripheral blood T lymphocytes and immature thymocytes. transfectants and recombinant CD147. basophils and mast cells. TRAP1.3.6 binds to CD154 at an epitope distinct Quantity: 100 µg Clone AD2-13H12 had been shown to inhibit the binding of HSB T-cells from the CD40 binding site. to IFN gamma treated keratinocytes when used in combination with Use: C, IP, WB CD2 or CD58 antibodies (1). Removal of sodium azide is recommended Quantity: 100 µg Use: C CD162 prior to use in functional assays. Quantity: 200 µg Use: C, F, E, WB CD150 Cat. no. MON 9053 (Monoclonal) NEW Quantity: 100 µg CD154, conjugated Also available as FITC conjugate (MON 4070F - 100 µg) Clone TB5 Also available as R-PE conjugate (MON 4070R - 100 tests) Cat.no. MON 4095 (Monoclonal) NEW The antibody recognizes the CD162 antigen. Cat.no. MON 4075B/F (Monoclonal) NEW Clone A12 Use: C CD166 A12 recognizes human CD150, a 70kD cell surface glycoprotein also Clone MK13A4 Quantity: 100 µg known as signalling lymphocytic activation molecule (SLAM). CD150 is MK13A4 recognizes the human CD154 cell surface antigen, a 32kD constitutively expressed on CD45+ ve T-cells, immature thymocytes, a glycoprotein also known as CD40 ligand. CD154 is expressed on Cat.no. MON1172 (Monoclonal) subpopulation of peripheral B-cells and dendritic cells. CD150 is rapidly activated T lymphocytes, predominantly CD4+ve cells and also on some CD163 upregulated in T-cells, B-cells and dendritic cells after activation. In basophils and mast cells. Clone MK13A4 has been reported to block Clone L50 addition to the membrane form of CD150, activated T and B cells also binding of CD40 ligand to its receptor CD40. The clone was obtained by screening hybridoma supernatants in ELISA express mRNA encoding a secreted form of CD150. Cat. no. MON 3096 (Monoclonal) NEW against purified recombinant Fc ALCAM. Fc ALCAM consists of the Fc This clone is reported to induce proliferation of pre-activated T cells. Use: C part of human immunoglobulin and the extra cellular part of ALCAM. Quantity: 100 tests Clone RM3/1 The monoclonal is positive on K562-cells transfected with C-DNA Use: C, IP Available as BIOTIN conjugate (MON 4075B - 100 tests) The monoclonal antibody RM3/1 recognizes CD163, a membrane encoding ALCAM and is negative on K562-cells. The monoclonal is Quantity: 100 µg Available as FITC conjugate (MON 4075F - 100 tests) glycoprotein of 130 kD, which is expressed exclusively on the cell surface reactive with Fc-ALCAM on Western Blot. It blocks ALCAM-ALCAM Also available as BIOTIN conjugate (MON 4095B - 100 µg) of human monocytes and macrophages that predominantly evolve in interactions and is reactive with the C-type domains. Also available as FITC conjugate (MON 4095F - 100 µg) the late phase of inflammation. The CD163 is a member of the CD158b scavenger receptor cysteine-rich superfamily. CD163 is a scavenger Use: F, C, WB receptor for the hemoglobin-haptoglobin complex. The protein expression Quantity: 1 ml CD152 is markedly induced by glucocorticoids while it is down-regulated by Cat.no. MON4061 (Monoclonal) NEW cyclosporin A and by phorbol esters. CD163 is sensitive to ionic detergents and to chloroform/methanol treatment. Positive staining CD173 Cat.no. MON4063 (Monoclonal) NEW Clone GL183 can be observed in the skin (histiocytes), gut, Kupffer cells, few alveolar GL183 recognizes the human CD158b cell surface antigen, a 58kD macrophages, a main population of macrophages in the placenta, Clone BNI3 glycoprotein expressed by a subset of NK cells (also known as p58.2 varying degrees of macrophages in inflamed tissue, including tumorous Cat.no. MON 4078 (Monoclonal) NEW BNI3 recognizes the human CD152 cell surface antigen, also known as antigen). This antibody also recognizes a 50kD molecule, which is highly tissue depending on the inflammatory stage. Red pulp, but not white CTLA-4 (cytotoxic T-lymphocyte-associated antigen 4). CD152 is a 45kD homologous to p58.2 in the extracellular domain, but has a shorter pulp macrophages of the spleen, and cortical macrophages of the thymus Clone BRIC231 glycoprotein, expressed normally as a disulphide-linked homodimer. It cytoplasmic tail. are detected. Macrophages in the synovialis of patients with rheumatoid This antibody recognizes the type 2 H blood group antigen, recently is expressed transiently on the surface of T cells following activation Both molecules are members of the newly described natural killer cell arthritis; in alveolar macrophages and in Kupffer cells a double staining designated CD173. Active H substances in man, are expressed by many (peaking at 3 days), but is often present with the cytoplasm. CD152 is receptor family. CD158b functions as a receptor specific for HLA Class I can be observed with the monoclonal antibody 25F9 (HM2158) which cells and tissues and also by erythrocytes. a ligand for CD80 and CD86, and is important in cellular interactions molecules, including Cw3 and related HLA-C alleles. This antibody can is not the case in other tissues. The monoclonal antibody RM3/1 is very during the immune response. restore the lysis by human NK clones of otherwise lysis protected targets useful for macrophage phenotyping. The antibody cross reacts with Use: C expressing Cw3. monkey. Quantity: 200 µg Use: C, F Quantity: 200 µg Use: C, IP Use: WB, C, E, IP, F, P Quantity: 200 µg Quantity: 100 µg

64 www.monosan.com For research use only. Not for drug, diagnostic or other use. 65 Cluster Differentiation Markers Cluster Differentiation Markers

CD174 CD180 CD200R CD210

Cat.no. MON 4089 (Monoclonal) NEW Cat.no. MON 4087 (Monoclonal) NEW Cat.no. MON 5095 (Monoclonal) NEW Cat.no. MON 4097 (Monoclonal) NEW

Clone A7O-C/C8 Clone MHR73 Clone OX-108 Clone 3F9 The antibody recognizes the human CD174 cell surface antigen, also MHR73 recognizes human CD180 (RP105) a 105kD type 1 trans- OX-108 recognizes human CD200R, a cell surface glycoprotein 3F9 recognizes human CD210, a 90-110kD glycoprotein, also known known as Lewis Y. CD174 is a difucosylated tetrasaccharide found on membrane protein, which belongs to the toll-like receptor family. CD180 (also known as OX2R). In humans CD200R is expressed primarily by as Interleukin-10 receptor (IL-10R). CD210 is expressed on T cells, NK the type 2 blood group oligosaccharides of glycolipids and glycoproteins. is primarily expressed in mature peripheral B cells and is thought to peripheral blood monocytes and neutrophils but also by other leucocytes cells, monocytes and dendritic cells, although surface expression may On epithelial ovarian cancer the predominant carriers of the CD174 play a role in the innate immune response to bacterial lipopolysaccharide, including T-lymphocytes and mast cells. Studies suggest that CD200- be low. Recent work has reported an increase in intracellular expression epitope are CA125 and MUC1. On CD34+ stem cells the carrier is a through co-operation with MD-1 and TLR4. Cross linking of CD180 CD200R interaction may be involved in the control of myeloid cellular of CD210 during dendritic cell maturation, but a reduction in surface 170kD glycoprotein designated E3. with clone MHR73 has been reported to induce resistance against function. Levels of expression on resting perispheral blood cells are CD210 expression. irradiation induced apoptosis, B-cell proliferation and up-regulation of relatively low. Use: C, F, P, E a co-stimulatory molecule CD80. Use: C Quantity: 1 ml Use: C Quantity: 100 µg Use: C, F, IP Quantity: 200 µg Also available as BIOTIN conjugate (MON 4097B - 100 µg) Quantity: 200 µg Also available as FITC conjugate (MON 5095F - 100 µg) Also available as R-PE conjugate (MON 4097R - 100 tests) CD175 Also available as FITC conjugate (MON 4087F - 100 µg) CD205 CD222 Cat.no. MON 4079 (Monoclonal) NEW CD195 Clone BRIC 111 Cat.no. MON 4099 (Monoclonal) NEW Cat.no. MON2064 (Monoclonal) BRIC111 recognizes Tn antigen on glycophorin A and glycophorin B in Cat.no. MON 4091 (Monoclonal) NEW human erythrocytes. Tn is a cryptantigen which was recently designated Clone MG38 Clone MEM-238 CD175 at the 7th Leucocyte Typing Workshop. Tn antigen is not Clone HEK/1/85a MG38 recognizes human CD205, a 205kD cell surface glycoprotein This antibody recognizes the human CD222 antigen. MEM-238 expressed on normal haemopoietic cells but exposure of the Tn is HEK/1/85a recognizes the human CD195 cell surface antigen, a 45kD that is also known as DEC-205. CD205 is a multilectin receptor which recognizes an epitope between domains 2 and 5. associated with polyagglutination. glycoprotein also known as CCR5. CD195 acts as a receptor for a in humans is predominantly expressed by dendritic cells. Clone MG38 number of chemokines including RANTES and eotaxin and also serves stains mature monocyte-derived dendritic cells and weakly stains some Use: C Use: F as a co-receptor for the entry of HIV into cells. CD195 is expressed by peripheral blood mononuclear cells. Clone MG38 also stains cortical Quantity: 100 µg Quantity: 200 µg a subset of T lymphocytes and by monocytes. epithelium in the thymus.

Use: C Use: C, F, IP CD226 CD176 Quantity: 100 µg Quantity: 200 µg Also available as BIOTIN conjugate (MON 4091B - 100 µg) Also available as FITC conjugate (MON 4099F - 100 µg) Also available as FITC conjugate (MON 4091F - 100 µg) Also available as R-PE conjugate (MON 4099R - 100 tests) Cat.no. MON 4098 (Monoclonal) NEW Cat.no. MON 4090 (Monoclonal) NEW Also available as R-PE conjugate (MON 4091R - 100 tests) Clone DX11 Clone A78-G/A7 CD206 DX11 recognizes human CD226, a 65kD glycoprotein, also known as The antibody recognizes the human CD176 cell surface antigen, also CD200 DNAM1 (DNAX accessory molecule-1). CD226 is broadly expressed on known as the Thomsen-Friedenreich (TF) antigen. CD176 is a T-cells, NK cells, platelets, monocytes and a subset of B cells. CD226 is carbohydrate epitope carried by a variety of carrier proteins, such as Cat.no. MON 4088 (Monoclonal) NEW also expressed by a subset of CD3 positive thymocytes. Clone DX11 is MUC1. The epitope is expressed by varying levels by carcinomas (e.g. Cat.no. MON4073 (Monoclonal) NEW reported to inhibit T- and NK cell mediated cytotoxicity against tumour upon 85% of breast carcinoma), but it is masked or absent on almost Clone 15-2 cell targets and to block TNF alpha and IFN gamma secretion by all normal adult tissues. CD176 is also expressed by some CD34+ stem Clone MRC OX104 Clone 15-2 recognizes human mannose receptor, also known as CD206, alloantigen-specific T-cells. cells and by the KG1 cell line. MRC OX104 recognizes the human CD200 cell surface antigen, also which is a 175kd transmembrane protein belonging to the group of known as OX2. CD200 is expressed by a subset of B lymphocytes, some pattern recognition receptors. CD206 is predominantly expressed in Use: C, IP Use: C, F, P, E endothelial cells and by neurons. Recent studies have suggested that tissue macrophages and dendritic cells and can also be found in a Quantity: 100 µg Quantity: 1 ml the CD200-CD200 ligand system is of importance in the control of subpopulation of endothelial cells and sperm cells. CD206 is thought Also available as BIOTIN conjugate (MON 4098B - 100 µg) macrophage and granulocyte activation. to play a role in the innate and adaptive immune response Also available as FITC conjugate (MON 4098F - 100 µg) Also available as R-PE conjugate (MON 4098R - 100 tests) CD177 Use: C, F Use: C, F, WB Quantity: 100 µg Quantity: 200 µg Also available as BIOTIN conjugate (MON 4073B - 100 µg) Cat.no. MON2063 (Monoclonal) Also available as FITC conjugate (MON 4073F - 100 µg) Also available as R-PE conjugate (MON 4073R - 100 tests) Clone MEM-166 This antibody recognizes the human CD177 antigen.

Use: C, IP, WB Quantity: 100 µg

66 For research use only. Not for drug, diagnostic or other use. www.monosan.com 67 Cluster Differentiation Markers Cluster Differentiation Markers

CD227 CD235a / Glycophorin A CD239

Cat.no. MON4064 (Monoclonal) NEW Cat. no. MON 4029 (Monoclonal) NEW Cat.no. MON 4076 (Monoclonal) NEW

Clone C595 (NCRC48) Clone HIR2 Clone BRIC221 C595 (NCRC48) recognizes CD227, the breast cancer associated mucin The antibody recognizes N-terminal, homologous portion of glycophorins The antibody recognizes a non-polymorphic determinant on both encoded by the Muc-1 gene. In normal tissues expression is restricted A (GPA) and B (GPB) which are single-pass membrane sialoglycoproteins. the 85 and 78kD Lutheran (Lu) glycoproteins. BRIC 221 specifically to specialised glandular epithelial. Clone C595 recognizes the peptide GPA is the carrier of blood group M and N specificities, while GPB recognizes an epitope in the fourth extracellular domain of Lu epitope ARG-PRO-ALA-PRO within the protein core of the mucin. Anti- accounts for S, s and U specificities. GPA and GPB provide the cells with glycoprotein. Lutheran glycoprotein is a member of the immunoglobulin idiotypic antibodies to C595 (NCRC48) are available, and the primary a large mucin-like surface and it has been suggested this provides a superfamily and has recently been designated CD239 (B-CAM) at the sequence of the variable region of this antibody has been determined. barrier to cell fusion, so minimizing aggregation between red blood cells 7th leucocyte typing workshop. CD239 is expressed by erythrocytes in in the circulation. The antibody agglutinates untreated RBCs but it the peripheral blood. Use: C, F, P, E, WB failes to agglutinate papain-treated cells. The antibody HIR2 significantly Quantity: 200 µg binds to GPA, but weakly to GPB. The antibody is useful in erythroid Use: C, WB Also available as FITC conjugate (MON 4064F - 100 µg) cell development studies, because HIR2 antigen is expressed on early Quantity: 200 µg erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other CD229 cells (mature erythrocytes are characteristically CD235a positive and CD247 CD45 and CD71 negative).

Cat.no. MON 4085 (Monoclonal) Use: F, C Cat.no. MON4049 (Monoclonal) NEW Quantity: 100 µg Clone HLy 9.1.25 Clone G3 The antibody recognizes the human CD229 cell surface antigen, a 100- The T cell antigen receptor (TCR) consists of a ligand-specific alpha/ 120kD cell surface glycoprotein that is the homologue of murine Ly-9. CD236 beta heterodimer non-covalently associated with five invariant chains CD229 is a member of the immunoglobulin superfamily and may have including the CD3 gamma/delta/eta and zeta subunits, all of which are a function in cell signalling. The antigen is expressed at low levels by required for efficient surface expression. T cell activation through the mature T and B lymphocytes and at higher levels by single-positive Cat.no. MON 4077 (Monoclonal) NEW TCR induces cellular differentiation and/or proliferation and the thymocytes. Expression at variable levels is also seen in some cell lines, production of lymphokines and cytokines. Both the CD3 and TCR zeta such as Daudi, U937 and HUT78. Clone BRIC100 subunits are proposed to be responsible for the intracellular signal This antibody recognizes a 17 amino acid residue within the cytoplasmic transduction events. A novel monoclonal antibody, named G3, has been Use: C, IP domain of glycophorin C (GPC), which has recently been designated developed that is specific for the TCR zeta chain subunit (CD247). The Quantity: 200 µg CD236. antibody is capable of precipitating both non-phosphorylated and Also available as BIOTIN conjugate (MON 4085B - 100 µg) tyrosine phosphorylated TCR zeta chain. Also available as FITC conjugate (MON 4085F - 100 µg) Use: F, IP, WB Also available as R-PE conjugate (MON 4085R - 100 tests) Quantity: 200 µg Use: C, F, E, IP, WB Quantity: 200 µg Also available as FITC conjugate (MON 4049F - 100 µg) CD230 (aa109 – 112) CD238 Also available as R-PE conjugate (MON 4049R - 100 tests)

Cat.no. MON 4092 (Monoclonal) NEW Cat.no. MON 4081 (Monoclonal) NEW

Clone 3F4 Clone BRIC 203 3F4 is specific for amino acid 109 - 112 of human CD230, also known BRIC203 recognizes human CD238, also known as the kell blood group as prion protein. This clone recognizes both protease sensitive and antigen Kpbc. The kell blood group antigens are carried on a 93kD protease resistant forms of CD230. CD230 is a large membrane protein erythrocyte membrane glycoprotein. In addition clone BRIC203 will expressed normally in neurons and is thought to be involved in synaptic agglutinate Kp (a+b-c+) and Kp (a-b-c+) erythrocytes but does not react transmission. In certain diseases such as Creutzfeld-Jakob disease (CJD) with Ko erythrocytes. and Bovine Spongiform Encephalopathy (BSE), normal prion protein (PrPc) is transformed into an altered pathogenic form (PrPsc). This altered Use: IP form is resistant to proteolytic degradation and accumulates in the brains Quantity: 200 µg of affected individuals.

Use: F, P, E, IP, WB Quantity: 100 µg

68 For research use only. Not for drug, diagnostic or other use. www.monosan.com 69 Collagens Collagens

Collagen I Collagen I, Rat Collagen I, II, III, IV and V Collagen II

Cat.no. PS041 (Polyclonal) Cat.no. PS065 (Polyclonal) Cat.no. PS046 (Polyclonal) Cat.no. PS048 (Polyclonal)

The rabbit antibody recognizes the human collagen I. The antiserum was The rabbit antibody recognizes the rat collagen I. The antisera fraction The rabbit antibody recognizes the human collagen types I-V. They are The rabbit antibody recognizes the human collagen type II. The antisera subjected to immune-absorption with immobilized human serum proteins is absorbed with immobilized rat collagen types III to remove cross raised in rabbits. Specificity was assertained by ELISA and dot-blotting. fraction is absorbed with immobilized collagen types I, III, IV and V to to remove non-specific reacting antibodies. The specificity of the antibody reactive antibodies to antigenic determinants common for this type of remove cross reactive antibodies to antigenic determinants common was ascertained by competition ELISA. Minimal cross reactions with collagen. Specificity was ascertained by competition ELISA. Complete Use: F, E for various collagen types. Specificity was ascertained by competition other collagens were only found if 5-10 times higher concentrations inhibition was found if the antibody was pre-incubated with rat collagen Quantity: 1 ml ELISA. Complete inhibition is found if the antibody was pre-incubated were used of other collagens. No reactions were found with fibronectin, type I. Inhibition by rat collagen type III is observed only at 10-20 times with collagen type II. Inhibition by other types of collagens is observed fibrinogen and laminin. higher concentration. No inhibition is found with fibrinectin. fibrogen only at 20-50 times higher concentration. and laminin. Collagen I, II and III Use: F, IF, E Use: F, IF, E Quantity: 1 ml Use: F, IF, E Quantity: 1 ml Quantity: 1 ml Cat.no. MON 4007 (Monoclonal)

Collagen I Clone MCI-HA Collagen III Collagen I and III, Dog The antibody reacts with native as well as with heat denatured human collagen types I, II, III, in RIA, ELISA, and immunoblot assays. The antibody Cat.no. PS060 (Polyclonal) does not react with other collagen types (IV and V). Human plasma Cat.no. PS043 (Polyclonal) Cat.no. PS069 (Polyclonal) proteins do not interfere with binding to collagen. The goat antibody recognizes the human collagen I. The antisera fraction The rabbit antibody recognizes the human collagen III. Collagen cross is absorbed with immobilized collagen types II, III, IV and V to remove The rabbit antibody recognizes the dog collagen I and III. Specificity Use: F, E reactions, caused by the presence of antibodies to common antigenic cross-reactive antibodies to antigenic determinants common for various was ascertained by direct ELISA using pure dog collagen types I and III Quantity: 200 µg determinants on various collagens were removed by immune absorption collagen types. Specificity was ascertained by competition ELISA. for coating micro plate wells. No binding to canine serum proteins is on immobilized collagens types I, II, IV and V. The specificity of the Complete inhibition is found if the antibody was pre-incubated with revealed at similar dilutions of the antibody. antibody was ascertained by competition ELISA. Complete inhibition collagen type I. Inhibition by other types of collagens is observed only Collagen II was found if the antibody was pre-incubated with collagen type III. at 20-50 times higher concentration. No inhibition was found with Use: F, IF, E Minimal cross reactions with other collagens were only found if 5-10 fibronectin, fibrinogen and laminin. Quantity: 1 ml times higher concentrations were used of other collagens. No reactions Cat.no. PS042 (Polyclonal) were found with fibronectin, fibrinogen and laminin. Use: F, IF, E Quantity: 1 ml Collagen I and III, Calf The rabbit antibody recognizes the human collagen II. The specificity of Use: F, IF, E the antibody was ascertained by competition ELISA. Complete inhibition Quantity: 1 ml was found if the antibody was pre-incubated with collagen type II. Collagen I Cat.no. PS068 (Polyclonal) Minimal cross reactions with other collagens were only found if 5-10 times higher concentrations were used of other collagens. No reactions Collagen III The rabbit antibody recognizes the calf collagen I and III. Specificity were found with fibronectin, fibrinogen and laminin. Cat.no. PS047 (Polyclonal) was ascertained by direct ELISA using pure calf collagen types I and III for coating micro plate wells. No binding to calf serum proteins is Use: F, IF, E Cat.no. PS062 (Polyclonal) The rabbit antibody recognizes the human collagen I. The antisera revealed at similar dilutions of the antibody. Quantity: 1 ml fraction is absorbed with immobilized collagen types II, III, IV and V to The goat antibody recognizes the human collagen type III. The antisera remove cross reactive antibodies to antigenic determinants common Use: F, IF, E fraction is absorbed with immobilized collagen types I, II, IV and V to for various collagen types. Specificity was ascertained by competition Quantity: 1 ml Collagen II remove cross reactive antibodies to antigenic determinants common ELISA. Complete inhibition is found if the antibody was pre-incubated for various collagen types. Specificity was ascertained by competition with collagen type I. Inhibition by other types of collagens is observed ELISA. Complete inhibition is found if the antibody was pre-incubated only at 20-50 times higher concentration. Collagen I and III, Pig Cat.no. PS061 (Polyclonal) with collagen type III. Inhibition by other types of collagens was observed only at 20-50 times higher concentration. No inhibition was found with Use: F, IF, E The goat antibody recognizes the human collagen type II. The antisera fibronectin, fibrinogen and laminin. Quantity: 1 ml Cat.no. PS067 (Polyclonal) fraction is absorbed with immobilized collagen types I, III, IV and V to remove cross reactive antibodies to antigenic determinants common Use: F, IF, E The rabbit antibody recognizes the pig collagen I and III. Specificity for various collagen types. Specificity was ascertained by competition Quantity: 1 ml was ascertained by direct ELISA using pure pig collagen types I and III ELISA. Complete inhibition is found if the antibody was pre-incubated for coating micro plate wells. No binding to pig serum proteins is with collagen type II. Inhibition by other types of collagens is observed revealed at similar dilutions of the antibody. only at 20-50 times higher concentration. No inhibition is found with fibronectin, fribrinogen and laminin. Use: F, IF, E Quantity: 1 ml Use: F, IF, E Quantity: 1 ml

70 www.monosan.com For research use only. Not for drug, diagnostic or other use. 71 Collagens Collagens

Collagen III Collagen IV Collagen IV Collagen V

Cat.no. PS049 (Polyclonal) Cat.no. PS044 (Polyclonal) Cat.no. PS050 (Polyclonal) Cat.no. PS045 (Polyclonal)

The rabbit antibody recognizes the human collagen type III. The antisera The rabbit antibody recognizes the human collagen IV. Collagen cross The rabbit antibody recognizes the human collagen type IV. The antisera The rabbit antibody recognizes the human collagen V. Collagen cross fraction is absorbed with immobilized collagen types I, II, IV and V to reactions, caused by the presence of antibodies to common antigenic fraction is absorbed with immobilized collagen types I, II, III and V to reactions, caused by the presence of antibodies to common antigenic remove cross reactive antibodies to antigenic determinants common determinants on various collagens were removed by immune remove cross reactive antibodies to antigenic determinants common determinants on various collagens were removed by immune absorption for various collagen types. Specificity was ascertained by competition absorption on immobilized collagens types I, II, III and V. The specificity for various collagen types. Specificity was ascertained by competition on immobilized collagens types I, II, III and IV. The specificity of the ELISA. Complete inhibition is found if the antibody was pre-incubated of the antibody was ascertained by competition ELISA. Complete ELISA. Complete inhibition is found if the antibody was pre-incubated antibody was ascertained by competition ELISA. Complete inhibition with collagen type III. Inhibition by other types of collagens is observed inhibition was found if the antibody was pre-incubated with collagen with collagen type IV. Inhibition by other types of collagens is observed was found if the antibody was pre-incubated with collagen type V. only at 20-50 times higher concentration. type IV. Minimal cross reactions with other collagens were only found only at 20-50 times higher concentration. Minimal cross reactions with other collagens were only found if 5-10 if 5-10 times higher concentrations were used of other collagens. times higher concentrations were used of other collagens. No reactions Use: F, IF, E No reactions were found with fibronectin, fibrinogen and laminin. Use: F, IF, E were found with fibronectin, fibrinogen and laminin. Quantity: 1 ml Quantity: 1 ml Use: F, IF, E Use: F, IF, E Quantity: 1 ml Quantity: 1 ml Collagen III, Rat Collagen IV Collagen IV Collagen V Cat.no. PS066 (Polyclonal) Cat.no. MON 4003 (Monoclonal)

The rabbit antibody recognizes the rat collagen type III. The antisera Cat.no. PS057 (Polyclonal) Clone 1042 Cat.no. PS051 (Polyclonal) fraction is absorbed with immobilized rat collagen types I to remove This monoclonal antibody reacts exclusively with human collagen type IV. cross reactive antibodies to antigenic determinants common for this This rabbit antibody reacts exclusively with collagen IV in ELISA and In immunoblots the antibody is exclusively reactive with basal The rabbit antibody recognizes the human collagen type V. The antisera type of collagen. Specificity was ascertained by competition ELISA. has a slight cross reaction with collagen V in immunoblotting. The membranes. There is no cross reactivity with any other collagen types. fraction is absorbed with immunobilized collagen types I, II, III and IV Complete inhibition was found if the antibody was pre-incubated with antiserum is important in the differential diagnosis of bullous lesions in Antigen isolated from: human placenta. Antigen location: extra cellular, to remove cross reactive antibodies to antigenic determinants common collagen rat type III. Inhibition by rat collagen type I is observed only dermatopathology. Antigen origin: human placenta. Antigen location: basement membranes, positive control: skin, kidney. for various collagen types. Specificity was ascertained by competition at 10-20 times higher concentration. No inhibition was found with extra cellular. Cross reactive with basement membrane of most species. ELISA. Complete inhibition is found if the antibody was pre-incubated fibrinectin. fibrogen and laminin. Use: F, P with collagen type V. Inhibition by other types of collagens is observed Use: F, P, E, WB Quantity: 1 ml only at 20-50 times higher concentration. Use: F, IF, E Quantity: 0.25 ml Quantity: 1 ml Use: F, IF, E Collagen IV Quantity: 1 ml Collagen IV Collagen III Cat.no. MON 4006 (Monoclonal) Collagen V Cat.no. PS063 (Polyclonal) Cat.no. MON 4013 (Monoclonal) Clone MC4-HA The goat antibody recognizes the human collagen type IV. The antisera This antibody reacts with native human collagen type IV, and with Cat.no. PS064 (Polyclonal) Clone MC3-HA fraction is absorbed with immobilized collagen types I, II, III, and V to heat-denatured collagen IV form in ELISA and immunoblots. There is This monoclonal antibody reacts exclusively with human collagen remove cross reactive antibodies to antigenic determinants common no cross reactivity with human collagen I,II,III and V. It stains exclusively The goat antibody recognizes the human collagen type V. The antisera type III in RIA, ELISA and dot blot. It lacks cross reactivity with any other for various collagen types. Specificity was ascertained by competition basement membranes on cryostat sections of human tissues such as fraction is absorbed with immunobilized collagen types I, II, III, and IV collagen type and plasma proteins. ELISA. Complete inhibition was found if the antibody was pre-incubated kidney, liver and heart. The antibody is important in the differential to remove cross reactive antibodies to antigenic determinants common with collagen type IV. Inhibition by other types of collagens is observed diagnosis of bullous lesions in dermatopathology. In in vitro studies for various collagen types. Specificity was ascertained by competition Use: F, E, WB only at 20-50 times higher concentration. No inhibition was found with with endothelial cells and smooth muscle cells the antibody is helpful ELISA. Complete inhibition was found if the antibody was pre-incubated Quantity: 1 ml fibronectin, fibrinogen and laminin. in detection of production collagen type IV. This antibody reacts also with collagen type V. Inhibition by other types of collagens is observed with dog collagen IV on cryostat and paraffin sections. only at 20-50 times higher concentration. No inhibition was found Use: F, IF, E with fribronectin, fibrinogen and laminin. Quantity: 1 ml Use: F, P, E Quantity: 200 µg Use: F, IF, E Quantity: 1 ml

72 For research use only. Not for drug, diagnostic or other use. www.monosan.com 73 Collagens Cytokines

Collagen V GM-CSF, Mouse Interferon-alpha, Mouse

Cat.no. MON 4008 (Monoclonal) Cat.no. MON 7033 (Monoclonal) Cat.no. MON 5067 (Monoclonal)

Clone MC5-HA Clone HP1-22E9 Clone F18 The antibody reacts with native human as well as heat denatured human This anti-GM-CSF is specific for natural and recombinant mouse GM-CSF. This monoclonal antibody (subclass IgG1) binds and neutralizes both collagen type V in RIA, ELISA and immunoblot assays. The antibody It inhibits the induction of the proliferation of BCL1-cells by GM-CSF natural and recombinant mouse alpha Interferon. It may be used for does not react with collagen types I, II, III and IV. Human plasma proteins (O’Garra A., et al., Cellular Immunology, 123, 189, 1989). GM-CSF is a biological assays as well as for purification. With the availability of do not interfere with binding to collagen. 23 kDa glycoprotein with diverse effects on immune and non-immune monoclonal antibodies directed against alpha Interferon, it is possible cells. It induces differentiation of granulocyte, macrophage and to interpret results obtained from crude materials containing both Use: F, E eosinophil precursor cells. Proliferation of monocyte macrophages, alpha and beta Interferon. The difficulties in studying in vitro and in Quantity: 200 µg T-lymphocytes, keratinocytes and endothelial cells is stimulated by vivo effects of “type 1” Interferons arise from the fact that both alpha GM-CSF. In addition, GM-CSF alters the functional properties of mature and beta Interferons are produced in response to the same stimuli and granulocytes, macrophages, eosinophils and basophils. GM-CSF is also seem to act via the same receptor. These Interferon activities can produced by T-lymphocytes, macrophages and several cell types in only be distinguished from one another by use of specific neutralizing extramedullary sites, where it may act in a paracrine manner to regulate antibodies. the local response to antigenic challenge (Ruef C., Coleman D.L., Ref. Infect. Dis., 12, 41, 1990). Use: Inh, IP, C, E Quantity: 200 µg Use: Inh., E, F Quantity: 100 µg Interferon-alpha I GM-CSF, Mouse Cat.no. MON 5086 (Monoclonal)

Cat.no. MON 7034 (Monoclonal) Clone 2-48 2-48 is specific for human interferon alpha 1 and does not cross react Clone MP1-13G6 with human interferon alpha 2. Source: A BALB/c mouse was immunized This anti-GM-CSF is specific for natural and recombinant mouse GM-CSF. with E.coli derived recombinant human interferon alpha 1. Splenocytes It inhibits the induction of the proliferation of BCL1-cells by GM-CSF were fused with NS-0 cells. (O’Garra A., et al., Cellular Immunology, 123, 189, 1989). GM-CSF is a 23 kDa glycoprotein with diverse effects on immune and non-immune Use: F, E, WB cells. It induces differentiation of granulocyte, macrophage and Quantity: 100 µg eosinophil precursor cells. Proliferation of monocyte macrophages, T-lymphocytes, keratinocytes and endothelial cells is stimulated by GM-CSF. In addition, GM-CSF alters the functional properties of mature Interferon-alpha I granulocytes, macrophages, eosinophils and basophils. GM-CSF is produced by T-lymphocytes, macrophages and several cell types in extramedullary sites, where it may act in a paracrine manner to regulate Cat.no. MON 5087 (Monoclonal) the local response to antigenic challenge (Ruef C., Coleman D.L., Ref. Infect. Dis., 12, 41, 1990). Clone 2-52 2-52 is specific for human interferon alpha 1 and does not cross react Use: Inh., E, F with human interferon alpha 2. Source: A BALB/c mouse was immunized Quantity: 100 µg with E-coli derived recombinant human interferon alpha 1. Splenocytes were fused with NS-0 cells.

Use: F, E, WB Quantity: 100 µg

74 www.monosan.com For research use only. Not for drug, diagnostic or other use. 75 Cytokines Cytokines

Interferon-alpha II Interferon-alpha IIB, Recombinant Interferon-gamma Interferon-gamma

Cat.no. MON 5085 (Monoclonal) Cat.no. MON 5058 (Monoclonal) Cat.no. MON 5071 (Monoclonal) Cat.no. MON 5082 (Monoclonal)

Clone N27 Clone IFNA2.3 Clone F1 Clone F14 The alpha interferons are involved in virus resistance in target cells for The Interferon alpha gene family is comprised of 16 or 17 gene copies Monoclonal antibody F-1 binds both natural and recombinant mouse Monoclonal antibody F14 binds and neutralizes both natural and these viruses. They are known to block cell proliferation and to regulate per haploid chromosome and a considerable allelic variation. So far gamma Interferon. Its binding activity has been demonstrated in vitro recombinant human gamma Interferon. Cross reactivity with other MHC class I antigen expression. The IFNα-family has pseudogenes in more than 30 different gene products have been identified all of which and in vivo (R. Dijkmans, et. al., J. Biol. Chem. 262, 2528-2535, 1987). cytokines has not been found. In general, monoclonal antibodies to two families (I and II), one with a mature length of 166aa and one of have the same similar biological properties although the specific F-1 antibodies have been demonstrated to be able to inhibit gamma Interferon are able to inhibit Shwartzman reactions (reviewed 172aa. Cells producing IFNα are lymphocytes, monocytes, macrophages activity may differ (De Maeyer E., De Maeyer-Guignard J., Interferons inflammatory responses to bacterial lipopoly-saccharides (Heremans et by A. Billiau, 1988, Imm. Today, 9, 37-40) and in the mouse system and cell lines such as Namalwa and KGI. Bioassays for IFNα include and Other Regulatory Cytokines, 1st ed., John Wiley & Sons, New York, al., J. Immunol., 138, 4175-4179, 1987). These antibodies were appear to protect NZB mice against spontaneous development of cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on 1988). This antibody is able to inhibit most of the biological effects of furthermore shown to inhibit Shwartzman reactions (Billiau et. al., autoimmune disease (Jacob et el., 1987, J. Exp. Med., 166, 798). their target cells. A number of receptors for IFNα are now known and alpha Interferon. The biological effects of Interferon alpha include EUR. J. Immunol., 17, 1851-1854, 1987) and to protect NZB mice against seem to be expressed on most cell types. N27 is specific for INFα(II) antiviral activity; anti-proliferate activity; stimulation of cytotoxic spontaneous development of autoimmune disease (Jacob et. al., J. Exp. Use: IP, F and does not cross react with INFα(I). Source: A BALB/c mouse was activities in lymphocytes, natural killer cells, and macrophages; and Med., 166, 798, 1987). The neutralizing activity of the F-1 antibody has Quantity: 100 µg immunized with E-coli derived, purified recombinant IFN-alpha 2c and modulation of the major histocompatibility complex. Particularly been demonstrated as being poor in anti-viral assays. spleen cells were fused with mouse myeloma cells NS1. important in the field of oncology is the fact that some tumour cell lines are very sensitive to the anti-proliferate effect of Interferon alpha. Use: E, IP Interferon-gamma Use: E, WB Quantity: 100 µg Quantity: 100 µg Use: N, IP Quantity: 100 µg Cat.no. PS 019 (Polyclonal) Interferon-gamma Interferon-alpha II The antibody reacts with human IFN-γ of both natural and recombinant Interferon-alpha IIB, Recombinant origin. IFN-γ is a pluripotent cytokine with important pro-inflammatory Cat.no. MON 5072 (Monoclonal) functions. The antibody inhibits the biological activity of natural and Cat.no. MON 5089 (Monoclonal) recombinant human IFN-γ. The antigen specificity was further assessed Cat.no. MON 5092 (Monoclonal) Clone F3 by ELISA and Western blot. No cross reactivities with other cytokines Clone N39 Monoclonal antibody F-3 binds and neutralizes both natural and have been detected. The alpha interferons are involved in virus resistance in target cells for Clone IFNA2.4 recombinant mouse gamma Interferon. Its binding and strong these viruses. They are known to block cell proliferation and to regulate The Interferon alpha gene family is comprised of 16 or 17 gene copies neutralizing activity has been demonstrated in vitro and in vivo Use: N, F, WB, E MHC class I antigen expression. The IFNα family has pseudogenes in per haploid chromosome and a considerable allelic variation. So far (R. Dijkmans, et. al., J. Biol. Chem. 262, 2528-2-535, 1987). F-3 antibodies Quantity: 100 µg two families (I and II), one with a mature length of 166aa and one of more than 30 different gene products have been identified all of which have been demonstrated to be able to inhibit inflammatory responses 172aa. Cells producing IFNα are lymphocytes, monocytes, macrophages have the same similar biological properties although the specific to bacterial lipopoly-saccharides (Heremans et al., J. Immunol., 138, and cell lines such as Namalwa and KGI. Bioassays for IFNα include activity may differ (De Maeyer E., De Maeyer-Guignard J., Interferons 4175-4179, 1987). These antibodies were furthermore shown to inhibit Interferon-gamma, Rat cytopathic effect blocking, by viruses such as VSV, SFV and BMCV, on and Other Regulatory Cytokines, 1st ed., John Wiley & Sons, New York, Shwartzman reactions (Billiau et. al., EUR. J. Immunol., 17, 1851-1854, their target cells. A number of receptors for IFNα are now known and 1988). The biological effects of Interferon alpha include antiviral 1987) and to protect NZB mice against spontaneous development of seem to be expressed on most cell types. N39 is specific for INFα(II) activity; anti-proliferate activity; stimulation of cytotoxic activities in autoimmune disease (Jacob et. al., J. Exp. Med., 166, 798, 1987). Cat.no. PS 006 (Polyclonal) and does not cross react with INFα(I). Source: A BALB/c mouse was lymphocytes, natural killer cells, and macrophages; and modulation of immunized with E-coli derived, purified recombinant IFN-alpha 2c and the major histocompatibility complex. Particularly important in the Use: IP, inh. The antibody reacts with rat IFN-γ of both natural and recombinant spleen cells were fused with mouse myeloma cells NS1. field of oncology is the fact that some tumour cell lines are very Quantity: 100 µg origin. IFN-γ is a pluripotent cytokine with important pro-inflammatory sensitive to the anti-proliferate effect of Interferon alpha. This antibody functions. The antibody inhibits the biological activity of natural and Use: E, WB, Blocking is able to inhibit most of the biological effects of alpha Interferon. recombinant rat IFN-γ. The antigen specificity was further assessed by Quantity: 100 µg Interferon-gamma ELISA and western blot. No cross reactivities with other cytokines have Use: IP been detected. Quantity: 100 µg Cat.no. MON 5093 (Monoclonal) Use: F, WB, E Quantity: 100 µg Clone F12 Monoclonal antibody F12 binds and neutralizes both natural and recombinant human gamma Interferon. Cross reactivity with other cytokines has not been found. Possible applications include the purification of human gamma Interferon and in vitro neutralization. In general, monoclonal antibodies to gamma Interferon are able to inhibit Shwartzman reactions (reviewed by A. Billiau, 1988, Imm. Today, 9, 37-40) and in the mouse system appear to protect NZB mice against spontaneous development of autoimmune disease (Jacob et al., 1987, J. Exp. Med., 166, 798).

Use: E, IP, F Quantity: 100 µg

76 For research use only. Not for drug, diagnostic or other use. www.monosan.com 77 Cytokines Cytokines

Interleukin-2 Interleukin-6 Interleukin-6 Interleukin-8R1 (CXCR1), CDw128

Cat.no. MON 5020 (Monoclonal) Cat.no. MON 5022S (Monoclonal) Cat.no. PS 037 (Polyclonal) Cat.no. MON 5049 (Monoclonal)

Clone F16 Clone 5E1 The antibodies react with human natural and recombinant IL6 as Clone NVR1 This antibody to recombinant human IL-2 exhibits neutralizing activity The antibody reacts with human native and recombinant IL-6 as assessed assessed by ELISA. The antibodies inhibit the biological activity of The antibody reacts with Human IL-8 Receptor type I better known as of the proliferating effect of recombinant IL-2 on IL-2 dependent cell by ELISA. The antibody inhibits the biological activity of human native human natural and recombinant IL6 as determined with the B9-cell CXCR1. There are two receptors for IL-8: CXCR1 (IL-8RA/R1) and lines. Interleukin 2 (IL-2) is characterized as a 55 Kd glycoprotein and recombinant IL-6 as determined with the B9 cell bioassay. The bio-assay. The antibodies cross react with baboon natural IL6. CXCR2 (IL-8RB/R2). One receptor, CXCR2, has high affinity for IL-8 and consisting of 251 amino acids. IL-2 is essential for the initiation and antibody cross reacts with rhesus, cynomolgous natural IL-6. It is all other CXC chemokines that attract neutrophils, while the other, maintenance of a normal immune response. The human high affinity sufficient for coating of at least 10 ELISA plates for IL-6 ELISA. Use: Inh., WB CXCR1 has high affinity for IL-8 only. IL-8 receptors are also found on Interleukin 2 receptor is a multimeric structure comprising at least two Quantity: 100 µg monocytes, basophils and eosinophils, but the responses of these cell molecular entities: the p55 chain (Tac antigen, a chain), which binds Use: Inh, WB, E to IL-8 are much weaker than those of neutrophils. IL-8 induces IL-2 with low affinity and the p75 component (chain), which represents Quantity: 200 µg neutrophil chemotaxis predominantly via Type 1 IL-8 Receptor CXCR1. the intermediate affinity receptor. The antibody recombinant human Interleukin-6, Mouse IL-2 is able to inhibit the binding of recombinant human IL-2 to its Use: Inh, C receptor. The antibody recombinant human IL-2 exhibits affinity for Interleukin-6, Mouse Quantity: 100 µg recombinant human IL-2. Cat.no. PS 014 (Polyclonal) Also available as BIOTIN conjugate (MON 5049B - 50 µg)

Use: Inh. Cat.no. MON 5073 (Monoclonal) The antibody reacts with natural and recombinant mouse IL-6 as Quantity: 100 µg assessed by ELISA. The antibody inhibits the biological activity of Interleukin-10, Mouse Clone MP5-20F3 natural and recombinant mouse IL-6 as determined with the B9-cell This anti-mIL-6 neutralizes specific mouse IL-6. It does not cross-react bio-assay. The antibody cross reacts with natural rat IL-6. Interleukin 6 Interleukin-2 with LPS (the component of the bacterial cell wall which is considered (IL-6) is a pluripotent 20-22 kDa cytokine which plays a role in the Cat.no. MON 5074 (Monoclonal) responsible for the toxicity of gram-negative bacteria) and TNF-α nor pathophysiology of severe infection and regulates the immune response, does it bind to either human or rat IL-6. The anti-mIL-6 inhibits IL-6 acute phase reaction and hematopoiesis. IL-6 plays a critical role in Clone JES5-2A5 Cat.no. PS 035 (Polyclonal) induced proliferation of the B9- assay. Interleukin 6 (IL-6) is a pluripotent B-cell differentiation to plasma cells and is a potent growth factor for This anti-mIL-10 neutralizes the biological activity of mouse IL-10. It 20-22 kDa cytokine which plays a role in the pathophysiology of plasmacytoma and myeloma. Continuous IL-6 gene expression makes does not cross react with Hu-IL-10. Mouse interleukin 10 (IL-10) is a

The antibodies react with and inhibit the biological activity of human severe infection and regulates the immune response, acute phase an essential contribution to a multi-step oncogenesis of plasma cell multifunctional 30-40 kDa cytokine which is produced by TH2 mouse Interleukin-2. It lacks reactivity with rat and mouse Interleukin-2. The reaction and haematopoiesis. IL-6 plays a critical role in B-cell neoplasia. IL-6 is a very useful culture supplement for the generation T- Helper cell clones and which inhibits the synthesis of several cytokines

antibody shows no detectable reactivity in RIA with human Interleukin-1, differentiation to plasma cells and is a potent growth factor for of a high number of antibody-producing hybridomas. by TH1-cells. Interleukin 10 was for this reason originally known as the TNF-alpha and IFN-gamma. plasmacytoma and myeloma. Continuous IL-6 gene expression makes Cytokine Synthesis Inhibitory Factor (CSIF). Interleukin 10 has activities an essential contribution to a multi-step oncogenesis of plasma cell Use: Inh., WB, E which involve a wide variety of cell types. Mouse IL-10 has been Use: IP, N neoplasia. IL-6 is a very useful culture supplement for the generation Quantity: 100 µg shown to exhibit extensive sequence similarity to Human IL-10 and to Quantity: 1 ml of a high number of antibody-producing hybridomas. an open reading frame in the Epstein-Barr virus genome BLRF-1. In contrast to Human IL-10 which is active on mouse cells, mouse IL-10 is Use: E Interleukin-8 not active in human cells. Given the aforementioned range of effects Interleukin-6 Quantity: 100 µg of IL-10 and its potential anti-inflammatory properties, antibodies capable of blocking the biological activity are clearly essential in the Cat.no. PS 038 (Polyclonal) study of these effects. Cat.no. MON 5022 (Monoclonal) Interleukin-6, Mouse The antibodies react with human natural and recombinant IL8 as Use: E, IP, N Clone 5E1 assessed by ELISA. The antibodies inhibit the biological activity of Quantity: 100 µg The antibody reacts with human native and recombinant IL-6 as assessed Cat.no. MON 5017 (Monoclonal) human natural and recombinant IL-8. The antibodies cross react with by ELISA. The antibody inhibits the biological activity of human native rhesus and cynomolgus natural IL-8. and recombinant IL-6 as determined with the B9-cell bioassay. The Clone MP5-32C11 Interleukin-10 antibody cross reacts with rhesus, cynomolgous natural IL-6. Anti-mIL-6 specifically neutralizes the biological activity of mouse IL-6. Use: WB, F, P It binds both natural and recombinant mouse IL-6 and does not cross Quantity: 100 µg Use: Inh, WB, F, E react to either human or rat IL-6. Interleukin 6 (IL-6) is a pluripotent Cat.no. PS 016 (Polyclonal) Quantity: 3 ml 20-22 kDa cytokine which plays a role in the pathophysiology of severe infection and regulates the immune response, acute phase reaction The antibody reacts with human IL-10 of both natural and recombinant and haematopoiesis. IL-6 plays a critical role in B-cell differentiation to origin. IL-10 is a pluripotent cytokine with important immunosuppressive

plasma cells and is a potent growth factor for plasmacytoma and actions: it can inhibit cytokines involved in the TH1 response such as myeloma. Continuous IL-6 gene expression makes an essential IL-2, IFN-γ and also inhibits the production of pro-inflammatory contribution to a multi-step oncogenesis of plasma cell neoplasia. cytokines such as IL-1, IL-6, IL-8 and TNF-α. The antibody inhibits the IL-6 is a very useful culture supplement for the generation of a high biological activity of natural and recombinant human IL-10. The antigen number of antibody-producing hybridomas. specificity was further assessed by ELISA. No cross reactivities with other cytokines have been detected. Use: Inh, E, IP Quantity: 100 µg Use: WB, E, Inh., F Quantity: 100 µg

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Interleukin-18, Mouse Monocyte Chematactic Protein-1 Tumour Necrosis Factor-alpha Tumour Necrosis Factor-alpha (MCP-1 or MCAF) Cat.no. PS 015 (Polyclonal) Cat.no. MON 7041 (Monoclonal) Cat.no. MON 7043 (Monoclonal) Cat.no. MON 7031 (Monoclonal) IL-18 also designated IFN-gamma Inducing Factor (IGIF) is a potent Clone 4C6-H8 Clone J2D10 IFN-gamma inducing cytokine. IL-18 has pluriform properties, it Clone MNA1 The mAb 4C6-H8 reacts with Tumour Necrosis Factor-alpha. It reacts on J2D10 reacts with human Tumour Necrosis Factor-alpha and not with induces activation of NF-kappa B, FAS-L expression and both CC and The antibody reacts with both human natural and recombinant MCP-1 paraffin sections with macrophages, in which the cytoplasma is stained. mouse TNF-alpha. This antibody neutralises Hu-rTNFa-mediated CXC chemokines production. The antibody is an essential tool for in ELISA. MCP-1 is a member of the CC subfamily of chemokines. Some keratinocytes are also positive (tonsils). Source: A BALB/c mouse cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects

investigations on TH1-type reactions in the mouse system. MCP-1 is a pro-inflammatory cytokine which acts both as an was immunized with synthetic TNF-alpha 115-130 hexadecapeptide mice against toxicity of Hu-rTNFa. Source: A BALB/c mouse was activation factor and as chemokine for monocytic cells. The antibody linked carrier. Splenocytes were fused with SP-2/0 Ag14 mouse immunized with human rTNF-alpha. Splenocytes were fused with NS1 Use: Inh., WB, E inhibits the biological activity of natural and recombinant human myeloma cells. mouse myeloma cells. Quantity: 100 µg MCP-1. The antigen specificity was assessed by ELISA. The antigen specificity was further assessed by immunoprecipitation and western Use: F, P, E Use: E, WB blot. No cross reactivities with other cytokines have been detected. Quantity: 100 µg Quantity: 100 µg IP-10 Use: F, WB, E Tumour Necrosis Factor-alpha Quantity: 100 µg Tumour Necrosis Factor-alpha Cat.no. MON 7036 (Monoclonal) Cat.no. MON 9068 (Monoclonal) Clone 6D4 Monocyte Chematactic Protein-1 Cat.no. MON 9061 (Monoclonal) The antibody reacts specifically with Human IP-10. Interferon-gamma- Clone T1 inducible 10 kD protein (IP-10), is a CXC chemokine with chemo (MCP-1 or MCAF) Clone 4H31 The antibody reacts with free soluble (17 kDa) and membrane (26 kDa) attractant properties for CD4-positive T-cells and inhibits early normal The antibody reacts with human native and recombinant TNF-α as human TNF-α. The antibody inhibits the biological activity of both forms. and leukemic hemopoietic progenitor proliferation. IP-10 is produced Cat.no. PS 007 (Polyclonal) assessed by ELISA. The antibody inhibits the biological activity of human It does not react with receptor bound TNF-α. by a wide variety of cell types ranging from neutrophils and monocytes native and recombinant TNF-α as determined with L929 cells in a to hepatocytes, endothelial cells and keratinocytes. The cytokine is The antibody reacts with human MCP-1 both natural and recombinant. cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus Use: Inh., IP, C, WB reported to be involved in a scala of inflammatory pathologies such as MCP-1 is a member of the CC subfamily of chemokines. MCP-1 is a natural TNF-α and lacks cross reactivity with human lymphotoxin. Quantity: 100 µg HIV encephalitis, coetaneous T-cell lymphoma, chronic hepatitis and pro-inflammatory cytokine which acts both as an activation factor for Also available as BIOTIN conjugate (MON 9068B - 50 µg) acute anterior uveitis. Various observations strongly suggest a role for monocytes and as chemokine for monocytic cells. The antibody inhibits Use: Inh., F, IP, C, WB, E the CXC chemokines IL-8 and IP-10 in the regulation of angiogenic the biological activity of natural and recombinant human MCP-1. The Quantity: 100 µg activity in cancer and in idiopathic pulmonary fibrosis. antigen specificity was assessed by ELISA. The antigen specificity was Tumour Necrosis Factor-alpha further assessed by immunoprecipitation and western blot. No cross Use: C, WB reactivities with other cytokines have been detected. Tumour Necrosis Factor-alpha Quantity: 100 µg Cat.no. MON 9067 (Monoclonal) Also available as BIOTIN conjugate (Mon 7036B - 50 µg) Use: F, WB, E, IP Quantity: 100 µg Cat.no. MON 7042 (Monoclonal) Clone T3 The antibody reacts with soluble (17 kDa), membrane (26 kDa) and MCP-1, Mouse Clone J1D9 receptor bound human TNF-α. The antibody inhibits the biological Tumour Necrosis Factor-alpha, Mouse J1D9 reacts with human Tumour Necrosis Factor-alpha and not with activity of soluble and membrane TNF-α. mouse TNF-alpha. This antibody neutralises Hu-rTNFa-mediated Cat.no. MON 7035 (Monoclonal) cytotoxicity of L929 cells and inhibits tumour growth in mice. It protects Use: IP, C, WB Cat.no. PS 052 (Polyclonal) mice against toxicity of Hu-rTNFa. Source: A BALB/c mouse was Quantity: 100 µg Clone ECE.2 immunized with human rTNF-alpha. Splenocytes were fused with NS1 Also available as BIOTIN conjugate (MON 9067B - 50 µg) The antibody reacts with mouse JE / MCP-1. Mouse MCP-1 is a potent Tumour Necrosis Factor alpha (TNF-α) is a cytokine which has diverse mouse myeloma cells. pro-inflammatory member of the CC chemokine family which immunomodulatory, anti-tumour and toxic effects. TNF-α has been corresponds with the product of the early response gene JE of the mouse. detected in diverse inflammatory status and appears to be a critical Use: E, WB MCP-1 is a chemokine that in addition to chemo taxis, induces mediator in the lethality of septic shock. Furthermore, TNF-α has also Quantity: 100 µg respiratory burst in monocytes, cytokine synthesis and enhances adhesion been found in inflammatory foci such as synovial effusions in molecule expression by monocytes. rheumatoid arthritis, systemic circulation in septic shock, parasitemia and rejection of renal transplants. Anti-mouse TNF-α reacts with both Use: WB, E, P, F rat and mouse natural and recombinant TNF-α. Anti-mouse TNF-α Quantity: 100 µg shows neutralizing activity. Also available as BIOTIN conjugate (Mon 7035B - 50 µg) Use: Inh., IP, WB, F Quantity: 100 µg

80 www.monosan.com For research use only. Not for drug, diagnostic or other use. 81 Cytokines Enzymes

Tumour Necrosis Factor-alpha Tumour Necrosis Factor-alpha Alkaline Phosphatase IAP, Intestinal Alkaline Phosphate

Cat.no. MON 9060 (Monoclonal) Cat.no. MON 5006 (Monoclonal) Cat.no. MON 8040 (Monoclonal) Cat.no. MON 5011 (Monoclonal)

Clone V1q Clone 52B83 Clone V17.1 Clone MIG-I15 Tumour Necrosis Factor alpha (TNF-α) is a cytokine which has various This antibody reacts with human native and recombinant TNF-α as V17.1 reacts with alkaline phosphatase, an enzym widely used in The antibody reacts with human Intestinal Alkaline Phosphatase; immunomodulatory, anti-tumour and toxic effects. TNF-α has been assessed by ELISA and lacks cross reactivity with TNF-β or lymphotoxin. immunodection tests. Source: A BALB/c mouse was immunized with no cross reactivity with other AP-isoenzymes was observed. detected in various inflammatory status and appears to be a critical It reacts with free soluble (17 kDa) and membrane (26 kDa) human alkaline phophatase. mediator in the lethality of septic shock. Furthermore, TNF-α has also TNF-α. It does not react with receptor bound TNF-α. The antibody Use: P been found in inflammatory foci such as synovial effusions in cross reacts with mouse, guinea pig and rhesus monkey TNF-α. Use: F, E Quantity: 1.5 ml rheumatoid arthritis, systemic circulation in septic shock, parasitemia Quantity: 100 µg and rejection of renal transplants. Anti-mouse TNF-α reacts with both Use: WB, F, P, Inh., E, C, IP natural and recombinant TNF-α and shows neutralizing activity. Quantity: 100 µg Ornithine decarboxylase Beta-Galactosidase from E. coli Use: Inh. Quantity: 100 µg Cat.no. PS 244 (Polyclonal) Cat.no. MON 5013 (Monoclonal) Immunogen: Recombinant human ODC produced in E. coli. Tested on Tumour Necrosis Factor-alpha Clone BG-02 mouse tissues. Positive control: Stefanini-fixed frozen sections of renal The antibody recognizes a non-catalytic center epitope of beta- cortex from testosterone-treated mice. galactosidase from E. Coli. The antibody reacts with lambda gt11 Cat.no. PS 011 (Polyclonal) fusion proteins. Use: F Quantity: 50 µl The antibody reacts with human natural and recombinant TNF-α as Use: WB, IC assessed by ELISA. The antibody inhibits the biological activity of Quantity: 100 µg human natural and recombinant TNF-α as determined with L929 and Ornithine decarboxylase WEHI cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural Histidine Decarboxylase, Guinea-Pig TNF-α and lack cross reactivity with human lymphotoxin. Cat.no. PS 259 (Polyclonal)

Use: WB, Inh., E, C Cat.no. PS 245 (Polyclonal) Immunogen: Recombinant human ODC produced in E. coli. Tested on Quantity: 100 µg mouse tissues. Positive control: Stefanini-fixed frozen sections of renal Immunogen: Recombinant histidine decarboxylase produced in E. coli. cortex from testosterone-treated mice. Absorption with 10-100 µg Tested on human, rat, mouse, dog, frog and chicken tissues. Positive immunogen per ml diluted antiserum abolishes the staining. Tumour Necrosis Factor-alpha control: Stefanini-fixed frozen sections of fundus from rat. Use: F Use: P, F Quantity: 50 µl Cat.no. PS 030 (Polyclonal) Quantity: 50 µl

The antibodies react with human natural and recombinant TNF-α as Placental Alkaline Phosphatase, PLAP assessed by ELISA. The antibodies inhibit the biological activity of Horseradish Peroxidase human natural and recombinant TNF-α as determined with L929 and WEHI cells in a cytotoxicity assay. The antibodies cross react with Cat.no. MON 9002 (Monoclonal) rhesus and cynomolgus natural Cat.no. MON 5041 (Monoclonal) TNF-α and lack cross reactivity with human lymphotoxin. Clone MIG-P1 Clone HP-03 The antibody reacts with human Placental Alkaline Phosphatase (hPLAP); Use: N, WB The antibody can be used for preparation of peroxidase-anti-peroxidase cross reactivity with other AP-isoenzymes is less than 0.01%. Reagents Quantity: 0.5 ml complexes (PAP). have been evaluated on several tissues known to be positive or negative for the antigen. No cross reaction has been found other then tissue Use: E, WB, IHC, IC non-specific AP in human granulocytes. Quantity: 100 µg Use: P Quantity: 1.5 ml

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Prostatic Specific Acid Phosphatase, PAP Urokinase, non-inhibitory Urokinase

Cat.no. MON 9009 (Monoclonal) Cat.no. MON 5033 (Monoclonal) Cat.no. PS 075 (Polyclonal)

Clone 4LJ Clone PGM2005 Antibodies to human Urokinase are raised in goats which are numerously Specificity for the 52 kDa PAP protein was tested by ELISA, immuno- The antibody binds to human high molecular weight single-chain immunized with extensively purified native HMW-tcuPA and LMW-scuPA blotting and immunochemistry. The antigen was isolated from human urokinase type plasminogen activator (HMW-scuPA, 54 kDa), to high (mixture 50/50), isolated from human urine. Pooled antisera are passed seminal plasma. Location of antigen, cytoplasm, extra cellular. molecular weight two-chain urokinase type plasminogen activator over DEAE-cellulose to produce IgG-enriched fraction, which is further (HMW-tcuPA, 52 kDa) and to low molecular weight single-chain subjected to absorption with immobilized total human serum proteins Use: F, P urokinase type plasminogen activator (LMW-scuPA, 33 kDa) with high in order to remove non-specific antibodies. The affinity purified antibody Quantity: 1 ml affinity (Kd= 0.1 nM) and does not inhibit the ability of urokinase to PS075 is obtained by binding to immobilized native human urokinase activate plasminogen. Urokinase reaction with synthetic substrate S2444 (antigen used for immunization), followed by elution with acidic buffer, is not inhibited. The antibody is highly specific for urokinase, no cross neutralization, dialysis, dispensing and lyophilization. Protein-tyrosine kinase (p561ck) reactions were found with other serine proteases (tissue plasminogen activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human The antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA whole plasma or plasma proteins do not interfere with its binding to (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity Cat.no. MON 2056 (Monoclonal) urokinase. Cross reacts with rat scu/tcu PA. (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is Clone LCK-01 Use: E, WB also inhibited. The antibody is highly specific for urokinase, no cross This antibody recognizes defined epitope (aa 22-36) on p561ck. Quantity: 200 µg reactions were found with other serine proteases (tissue plasminogen P561ck human protein kinase. activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human whole plasma or plasma proteins do not interfere with its binding to Use: IP, WB Urokinase urokinase. Quantity: 100 µg Use: F, P, WB Cat.no. PS 074 (Polyclonal) Quantity: 500 µg Urokinase, inhibitory Antibodies to human Urokinase are raised in rabbits which are numerously immunized with extensively purified native HMW-tcuPA Cat.no. MON 5018 (Monoclonal) and LMW-scuPA (mixture 50/50), isolated from human urine. Pooled antisera are passed over DEAE-cellulose to produce IgG-enriched Clone PGM2001 fraction, which is further subjected to absorption with immobilized The antibody binds to human high molecular weight single-chain total human serum proteins in order to remove non-specific antibodies. urokinase type plasminogen activator (HMW-scuPA, 54 kDa), to high The affinity purified antibody PS074 is obtained by binding to molecular weight two-chain urokinase type plasminogen activator immobilized native human urokinase (antigen used for immunization), (HMW-tcuPA, 52 kDa) and to low molecular weight single-chain followed by elution with acidic buffer, neutralization, dialysis, dispensing urokinase type plasminogen activator (LMW-scuPA, 33 kDa) with high and lyophilization. affinity (Kd= 0.1 nM) and does inhibit the ability of urokinase to activate plasminogen. Urokinase reaction with synthetic substrate S2444 is not The antibody binds to human HMW-scuPA (54 kDa), HMW-tsuPA inhibited. The antibody is highly specific for urokinase, no cross reactions (52 kDa) and LMW-scuPA (33 kDa) with high apparent affinity were found with other serine proteases (tissue plasminogen activator, (Kd= 0.1 nM) completely inhibiting the ability of urokinase to activate plasmin, trypsin, chymotrypsin, elastase, thrombin). Human plasma or plasminogen. Urokinase reaction with synthetic substrate S2444 is plasma proteins do not interfere with its binding to urokinase. also inhibited. The antibody is highly specific for urokinase, no cross reactions were found with other serine proteases (tissue plasminogen Use: E, WB activator, plasmin, trypsin, chymotrypsin, elastase, thrombin). Human Quantity: 200 µg whole plasma or plasma proteins do not interfere with its binding to urokinase.

Use: WB, F, P Quantity: 500 µg

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Capsaicin receptor Epidermal Growth Factor, EGF GnRH receptor Interleukin-1 Receptor Type I (p80), CDw121a, Mouse Cat.no. PS 264 (Polyclonal) Cat.no. MON8014 (Monoclonal) Cat.no. MON 8054 (Monoclonal) Cat.no. MON 5078 (Monoclonal) Immunogen: Synthetic peptide from the C-terminal of the capsaicin Clone F20 Clone A9E4 receptor conjugated to BSA. Works on rat tissues. Positive control: Frozen The epidermal growth factor (EGF) is a polypeptide consisting of A9E4 reacts with GnRH receptors in the anterior pituitary. GnRH Clone D14e3 sections of spinal cord from rat. Absorption with 10-100 µg immunogen 53 amino acids (MW 6 kD). EGF is able to stimulate cellular proliferation stimulates the gonadotrophs of the anterior pituitary to secrete This monoclonal antibody binds to soluble mouse interleukin-1 receptor per ml diluted antiserum abolishes the staining. of both ectodermic and mesodermic origin. This bioregulatory activity luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). type I. The IL-1 system includes two agonists (IL-1α and IL-1β), converting is carried out through a specific surface receptor. This receptor is a The receptor contains of seven hydrophobic transmembrane domains enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 Use: F glycoprotein consisting of 1186 amino acids (MW 170 kD) with a connected by hydrophillic extra cellular, and intracellular loops receptor accessory protein. Interleukin-1 signal is transduced through Quantity: 50 µl structural homology to the v-erbB oncogene (Carpenter G., Ann. Rev. characteristic of G-protein coupled receptors. Source: Mice were the type I receptor. Biochem., 56, 881, 1987). Hbt antibody to recombinant human EGF is immunized with a BSA-conjugated peptide corresponding to amino able to inhibit the binding of recombinant human EGF for the EGF acids 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) of human GnRH Use: WB CCK-A receptor, Rat receptor. Hbt antibody to recombinant human EGF exhibits very high receptor extra cellular domain. Splenocytes were fused with SP2/0 Quantity: 100 µg affinity for recombinant human EGF. Hbt antibody to recombinant human mouse myeloma cells. Stability was established by subcloning four times. EGF does not cross react with mouse EGF. Cat.no. PS 263 (Polyclonal) Use: F, C, E, WB Interleukin-1 Receptor Type I (p80), Use: Inh, E Quantity: 100 µg Immunogen: Synthetic peptide from the C-terminal of the rat CCK-A Quantity: 100 µg CDw121a, Mouse receptor. Tested on rat and mouse tissues. Positive control: frozen sections of rat pancreas. Absorption with 10-100 µg immunogen per ml diluted GnRH receptor Cat.no. MON 5076 (Monoclonal) antiserum abolishes the staining. Epidermal Growth Factor Receptor, EGFR Clone Reg20 Use: F Cat.no. MON 8056 (Monoclonal) This monoclonal antibody binds to soluble mouse interleukin-1 receptor Quantity: 50 µl Cat.no. MON 1033 (Monoclonal) type I. The IL-1 system includes two agonists (IL-1α and IL-1β), converting Clone F1G4 enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 Clone 2E9 F1G4 reacts with GnRH receptors in the anterior pituitary. GnRH receptor accessory protein. Interleukin-1 signal is transduced through Epidermal Growth Factor, EGF, Mouse The antibody reacts with a protein determinant of the extra cellular stimulates the gonadotrophs of the anterior pituitary to secrete the type I receptor. domain of the human EGFR, and does not cross react with EGFR from luteinising hormone (LH) as well as follicle-stimulating hormone (FSH). mouse cells. The antibody shows binding competition with EGF. The The receptor contains of seven hydrophobic transmembrane domains Use: WB, IP Cat.no. MON 8001 (Monoclonal) antibody reacts in immunoprecipitation with the functional EFR connected by hydrophillic extra cellular, and intracellular loops Quantity: 100 µg protein-tyrosine kinase complex. characteristic of G-protein coupled receptors. Source: Mice were Clone E5 immunized with a BSA-conjugated peptide corresponding to amino The antibody reacts with mouse EGF in ELISA (10 ng detectable) and Use: F, P, EM, IP acids 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) of human GnRH Interleukin-1 Receptor Type I (p80), in spot blots (1 ng detectable). In immunohistochemistry the antibody Quantity: 1 ml (MON 1033-1) receptor extra cellular domain. Splenocytes were fused with SP2/0 reacts with mouse salivary glands. No cross reaction with rat EGF. 5 ml (MON 1033-5) mouse myeloma cells. Stability was established by subcloning four times. CDw121a, Mouse

Use: E, F, P Use: F, C, E, WB Cat.no. MON 5075 (Monoclonal) Quantity: 1 ml (MON 8001-1) Fibronectin Receptor, VLA-alpha-5 Quantity: 100 µg 5 ml (MON 8001-5) Clone Reg21 This monoclonal antibody binds to soluble mouse interleukin-1 receptor Cat.no. MON 1034 (Monoclonal) Interleukin-1 Receptor Type I (p80), type I. The IL-1 system includes two agonists (IL-1α and IL-1β), converting Epidermal Growth Factor, EGF, Mouse enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 Clone NKI-SAM1 CDw121a, Mouse receptor accessory protein. Interleukin-1 signal is transduced through This antibody recognizes a 150/130 kD receptor for fibronectin, the type I receptor. Cat.no. MON 8002 (Monoclonal) (VLA-alpha-5 protein from the integrin super family of antigens). The Cat.no. MON 5077 (Monoclonal) antibody reacts with platelets, lymphocytes, monocytes and most cell Use: WB, IP, Inh. Clone F5 lines including K562 and U937. Clone D1f3 Quantity: 100 µg The antibody reacts with mouse EGF in ELISA and in spot blots. In This monoclonal antibody binds to soluble mouse interleukin-1 receptor immunohistochemistry the antibody reacts with formalin fixed and Use: F type I. The IL-1 system includes two agonists (IL-1α and IL-1β), converting paraffin embedded mouse salivary glands. It also reacts with human Quantity: 1 ml enzymes, antagonists, two receptors (IL-1 RI and IL-1 RII) and the IL-1 Brunner’s glands (presumably with urogastron). receptor accessory protein. Interleukin-1 signal is transduced through the type I receptor. Use: E, WB, F, P Quantity: 1 ml (MON 8002-1) Use: WB, IP, Inh. 5 ml (MON 8002-5) Quantity: 100 µg

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Interleukin-1 Receptor Type II, CDw121b Interleukin-1 Receptor type II Mannose Receptor Toll-Like Receptor 2 (TLR2)

Cat.no. MON 5084 (Monoclonal) Cat.no. PS029 (Polyclonal) Cat.no. MON 2073 (Monoclonal) Cat.no. MON 4030 (Monoclonal)

Clone 6G5 This polyclonal antibody reacts specifically with human IL-1 RII. The IL-1 Clone 15-2 Clone TL2.1 The antibody reacts specifically with Human IL-1 RII. The IL-1 system system includes two agonists (IL-1α and IL-1β), converting enzymes, The Mannose Receptor (MR), a member of the vertebrate C-type lectin Toll-like receptors (TLR) are highly conserved throughout evolution and includes two agonists (IL-1α and IL-1β), converting enzymes, antagonists, antagonistic, two receptors (IL-1 R1 and IL-1 RII) and the IL-1 receptor family, is a pattern recognition receptor that is involved in both innate have been implicated in the innate defence to many pathogens. In two receptors (IL-1 RI and IL-1 RII) and the IL-1 receptor accessory accessory protein. The IL-1 RII is part of the antagonistic IL-1 mechanism. and adaptive immunity. The 180 kDa transmembrane protein consists of Drosophila toll is required for the anti-fungal response, while the related protein. The IL-1 RII is part of the antagonistic IL-1 mechanism. It is It is also known as decoy receptor and is a non-signalling molecule 5 domains: an amino-terminal cysteine-rich region, a fibronectin type 18-wheeler is involved in antibacterial defences. In mammals, TLR also known as decoy receptor and is a non-signalling molecule which which functions by capturing IL-1 and preventing it from interacting II repeat, a series of eight tandem lectin-like carbohydrate recognition identified as type I transmembrane signalling receptors with pattern functions by capturing IL-1 and preventing it from interacting with the with the signalling IL-1 RI. The decoy IL-1 RII can after binding to IL-1 domains (responsible for the recognition of mannose and fucose), recognition capabilities, have been implicated in the innate host defence signalling IL-1 RI. The decoy IL-1 RII can after binding to IL-1 also recruit also recruit the IL-1 receptor accessory protein and thus inhibit by co a transmembrane domain, and an intracellular carboxy-terminal tail. to pathogens. TLR2 has been identified as a receptor that is central to the IL-1 receptor accessory protein and thus inhibit by co receptor receptor competition. Further a soluble form of IL-1 RII exists which is The structure is shared by the family of multi lectin mannose receptors: the innate immune response to lipoproteins of Gram-negative bacteria, competition. Further a soluble form of IL-1 RII exists which is shed, a shed, a process in which matrix metalloproteases have been found to the phospholipase A2-receptor, DEC 205 and the novel C-type lectin several whole Gram-positive bacteria, as well as a receptor for process in which matrix metalloproteases have been found to play a role, play a role, by various cells including monocytes, polymorphonuclear receptor (mannose receptor X). The MR recognizes a wide range of peptidoglycan and lipoteichoic acid and other bacterial cell membrane by various cells including monocytes, polymorphonuclear cells, B-cells cells, B-cells and fibroblatsts. gram positive and gram negative bacteria, yeasts, parasites and products. A functional interaction between TLR2 and TLR6 in the and fibroblasts. mycobacteria. The MR has also been shown to bind and internalize cellular response to various bacterial products has been discovered. Use: E tissue-type plasminogen activator. MR’s are present on monocytes and The currently accepted paradigm regards TLR2 as an essential receptor Use: E Quantity: 100 µg dendritic cells (DC) and are presumed to play a role in innate and for many eubacterial cell wall components, including lipoproteins and Quantity: 100 µg adaptive immunity, the latter via processing by DC. The expression of peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, MR as observed in immunohistology is present on tissue macrophages, mycoplasma, Staphylococcus aureus, and Streptococcus pneumonia Interleukin-1ra3 dendritic cells, a subpopulation of endothelial cells, Kupffer cells and have all been shown to mediate cellular activation via TLR2. The TL2.1 Interleukin-1 Receptor Type II sperm cells. The expression of MR on monocytes increases during culture monoclonal antibody is specific for human Toll-like receptor 2 (TLR2). and can be enhanced by cytokines as IFN-γ. Labeling of MR expressing TL2.1 is a TLR2 function blocking antibody that is useful for studies on Cat.no. MON 5080 (Monoclonal) monocytes/macrophages increases at 37°C with prolonged incubation the role of TLR2 as a pattern recognition receptor in microbial products Cat.no. MON 5079 (Monoclonal) time probably due to internalization of the MR-antibody-complex. The induced cytokine production by TLR2 bearing cells such as human Clone 2D11 antibody prevents binding of glycoproteins including t-PA to MR. peripheral blood mononuclear cells. The antibody can be used for Clone 8.5 This monoclonal antibody reacts specifically with human IL-1ra3. The Detection of the MR with anti-MR monoclonal antibody can substitute immunoprecipitation of human TLR2 (approximately 90 kDa). This monoclonal antibody reacts specifically with human IL-1 RII. The IL-1ra3 belongs to the IL-1 system which includes two agonists (IL-1α staining for mannose containing probes as labelled mannosylated IL-1 system includes two agonists (IL-1α and IL-1β), converting enzymes, and IL-1β), converting enzymes, antagonistic two receptors (IL-1 R1 BSA, a technique which is more cumbersome and less specific. Use: C, F, IP, WB antagonistic, two receptors (IL-1 R1 and IL-1 RII) and the IL-1 receptor and IL-1 RII) and the IL-1 receptor accessory protein. Three molecular Quantity: 100 µg accessory protein. The IL-1 RII is part of the antagonistic IL-1 mechanism. isoforms of the IL-1 receptor antagonist (IL-1ra) have been identified Use: F, C, WB Also available as BIOTIN conjugate (MON 4030B - 50 µg) It is also known as decoy receptor and is a non-signalling molecule and cloned. Secreted IL-1ra (sIL-1ra or IL-1ra1) contains a classical leader Quantity: 100 µg which functions by capturing IL-1 and preventing it from interacting peptide giving a released mature protein. Two intracellular isoforms, Also available as BIOTIN conjugate (MON 2073B - 50 µg) with the signalling IL-1 RI. The decoy IL-1 RII can after binding to IL-1 icIL-1ra type I (IL-ra2) and icIL-1 ra type II (IL-ra3), have no leader also recruit the IL-1 receptor accessory protein and thus inhibit by co sequence, thus predicting that these proteins remain intracellular. IL-1ra3 receptor competition. Further a soluble form of IL-1 RII exists which is may represent a reservoir of IL-1 inhibitors, released upon cell death, shed, a process in which matrix metalloproteases have been found to whose function is no limit the pro-inflammatory signals but most play a role, by various cells including monocytes, polymorphonuclear prominently by mononuclear phagocytes and keratinocytes. cells, B-cells and fibroblatsts. Use: WB, F, E Use: WB, C, E Quantity: 100 µg Quantity: 100 µg

Interleukin-2 Receptor alpha chain, CD25

Clone BL-D/D1 – Cat.no. MON 1044A (Monoclonal) Clone RO/G10 – Cat.no. MON 1044B (Monoclonal)

The antibodies react with the alpha chain of the Interleukin-2 Receptor (55 kD glycoprotein). CD25 is expressed on activated T-lymphocytes and B-lymphocytes. The antibody RO/G10 (Cat. no. MON 1044B) reacts earlier and shows earlier a strong signal on activated T-cells than the antibody BL-D/D1 (Cat. no. MON 1044A).

Use: IF, C Quantity: 1 ml

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Toll-Like Receptor 2 (TLR2) Toll-Like Receptor 2 (TLR2), Mouse Toll-Like Receptor 2 (TLR2), Mouse Toll-Like Receptor 4 (TLR4)

Cat.no. MON 4031 (Monoclonal) Cat. no. MON 4019 (Monoclonal) NEW Cat. no. MON 4025 (Monoclonal) NEW Cat.no. MON 4033 (Monoclonal)

Clone TL2.3 Clone T2.5 Clone mT2.7 Clone HTA125 Toll-like receptors (TLR) are highly conserved throughout evolution and Monoclonal antibody T2.5 reacts with mouse Toll-like receptor 2 (TLR2). Monoclonal antibody mT2.7 reacts with mouse Toll-like receptor 2 (TLR2). Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Toll-like receptors (TLR) are highly conserved throughout evolution and Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related have been implicated in the innate defense to many pathogens. In have been implicated in the innate defense to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related 18-wheeler is involved in antibacterial defences. In mammals, TLR Drosophila toll is required for the anti-fungal response, while the Drosophila toll is required for the anti-fungal response, while the 18-wheeler is involved in antibacterial defences. In mammals, TLR identified as type I transmembrane signalling receptors with pattern related 18-wheeler is involved in antibacterial defenses. In mammals, related 18-wheeler is involved in antibacterial defenses. In mammals, identified as type I transmembrane signalling receptors with pattern recognition capabilities, have been implicated in the innate host defence TLR identified as type I transmembrane signaling receptors with pattern TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host to pathogens. TLR2 has been identified as a receptor that is central to recognition capabilities, have been implicated in the innate host defense recognition capabilities, have been implicated in the innate host defense defence to pathogens. TLR4 has been identified next to MD-2 and the innate immune response to lipoproteins of Gram-negative bacteria, to pathogens. TLR2 has been identified as a receptor that is central to to pathogens. TLR2 has been identified as a receptor that is central to CD14 as a receptor that is central to the innate immune response to several whole Gram-positive bacteria, as well as a receptor for the innate immune response to lipoproteins of Gram-negative bacteria, the innate immune response to lipoproteins of Gram-negative bacteria, lipopolysaccharides (LPS) of Gram-negative bacteria. The HTA125 peptidoglycan and lipoteichoic acid and other bacterial cell membrane several whole Gram-positive bacteria, as well as a receptor for several whole Gram-positive bacteria, as well as a receptor for monoclonal antibody reacts preferentially, especially in flow cytometry, products. A functional interaction between TLR2 and TLR6 in the cellular peptidoglycan and lipoteichoic acid and other bacterial cell membrane peptidoglycan and lipoteichoic acid and other bacterial cell membrane with human TLR4 that is associated with MD-2. HTA125 is a TLR4 response to various bacterial products has been discovered. The products. A functional interaction between TLR2 and TLR6 in the products. A functional interaction between TLR2 and TLR6 in the function-blocking antibody that is useful for studies on the role of TLR4 currently accepted paradigm regards TLR2 as an essential receptor for cellular response to various bacterial products has been discovered. cellular response to various bacterial products has been discovered. as a receptor for LPS induced cytokine production by TLR4 bearing cells. many eubacterial cell wall components, including lipoproteins and The currently accepted paradigm regards TLR2 as an essential receptor The currently accepted paradigm regards TLR2 as an essential receptor The antibody was shown to precipitate TLR4 (100 kDa). peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, for many eubacterial cell wall components, including lipoproteins and for many eubacterial cell wall components, including lipoproteins and mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, peptidoglycan. Bacterial species as diverse as mycobacteria, spirochetes, Use: C, IP have all been shown to mediate cellular activation via TLR2. The TL2.3 mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae mycoplasma, Staphylococcus aureus, and Streptococcus pneumoniae Quantity: 100 µg monoclonal antibody is specific for human TLR2. TL2.3 is useful for have all been shown to mediate cellular activation via TLR2. have all been shown to mediate cellular activation via TLR2. Also available as BIOTIN conjugate (MON 4033B - 50 µg) studies on the role of TLR2 as a pattern recognition receptor in The T2.5 monoclonal antibody is reactive with mouse Toll-like receptor 2 The mT2.7 monoclonal antibody is specific for mouse Toll-like receptor 2 microbial products induced cytokine production by TLR2 bearing cells (TLR2). Furthermore T2.5 cross reacts with human TLR2. (TLR2). such as human peripheral blood mononuclear cells. Toll-Like Receptor 4 (TLR4)/MD-2, Mouse Use: C, F, IP Use: C, F, IP Use: C, F Quantity: 100 µg Quantity: 100 µg Quantity: 100 µg Also available as BIOTIN conjugate (MON 4019B - 50 µg) Also available as BIOTIN conjugate (MON 4025B - 50 µg) Cat.no. MON 4032 (Monoclonal) Also available as BIOTIN conjugate (MON 4031B - 50 µg) Clone MTS510 Toll-Like Receptor 3 (TLR3) Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defence to many pathogens. In Drosophila toll is required for the anti-fungal response, while the related Cat. no. MON 3068 (Monoclonal) NEW 18-wheeler is involved in antibacterial defences. In mammals, TLR identified as type I transmembrane signalling receptors with pattern Clone TLR3.7 recognition capabilities, have been implicated in the innate host Toll-like receptors (TLR) are highly conserved throughout evolution and defence to pathogens. TLR4 has been identified next to MD-2 and have been implicated in the innate defence to many pathogens. In CD14 as a receptor that is central to the innate immune response to Drosophila toll is required for the anti-fungal response, while the related lipopolysaccharides (LPS) of Gram-negative bacteria. The MTS510 18-wheeler is involved in antibacterial defences. In mammals, TLR monoclonal antibody reacts preferentially, especially in flow cytometry, identified as type I transmembrane signalling receptors with pattern with mouse TLR4 that is associated with MD-2. MTS510 is a TLR4 recognition capabilities, have been implicated in the innate host defence function-blocking antibody that is useful for studies on the role of TLR4 to pathogens. as a receptor for LPS induced cytokine production by TLR4 bearing cells. As investigated so far all functional characterized TLR signal via the The antibody was shown to co precipitate MD-2 (30 kDa) with TLR4 TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems (100 kDa). to be essential. Viral replication within infected cells results in generation of dsRNA Use: C, IP which can stimulate immune cells. Recent data show that TLR3 acts as Quantity: 100 µg a receptor involved in the recognition of dsRNA, indicating a role for Also available as BIOTIN conjugate (MON 4032B - 0.5 ml) TLR3 in viral recognition and innate defence.

Use: C, WB Quantity: 100 µg Also available as BIOTIN conjugate (MON 3068B - 50 µg)

90 www.monosan.com For research use only. Not for drug, diagnostic or other use. 91 Growth Factors & Receptors Growth Factors & Receptors

Toll-Like Receptor 9 (TLR9), Mouse Toll-Like Receptor 9 (TLR9) Transferrin Receptor, CD71 Transferrin Receptor 2 (TfR2)

Cat. no. MON 2068 (Monoclonal) NEW Cat. no. MON 2093 (Monoclonal) NEW Cat.no. MON 1009 (Monoclonal) Cat. no. MON 3087 (Monoclonal) NEW

Clone 5G5 Clone 5G5 Clone 66-IG10 Clone 9F8 1C11 Toll-like receptors (TLR) are highly conserved throughout evolution and Toll-like receptors (TLR) are highly conserved throughout evolution and The antibody reacts with the transferrin receptor and is positive with Human transferrin receptor 2 (TfR2), is a homodimeric type II membrane have been implicated in the innate defence to many pathogens. In have been implicated in the innate defence to many pathogens. In most proliferating cells. It reacts with a 190 kD molecule, which consists glycoprotein of 100-105 kDa. This receptor is most likely responsible for Drosophila toll is required for the anti-fungal response, while the related Drosophila toll is required for the anti-fungal response, while the related of two subunits of 95 kD. The gene coding for the antigen is located the non-TfR-meditated uptake of transferrin into cells and also plays a 18-wheeler is involved in antibacterial defences. In mammals, TLR 18-wheeler is involved in antibacterial defences. In mammals, TLR on the q-arm of chromosome 3. critical role in iron homeostasis. TfR2 is highly expressed in liver and identified as type I transmembrane signalling receptors with pattern identified as type I transmembrane signalling receptors with pattern peripheral blood mononuclear cells. recognition capabilities, have been implicated in the innate host defence recognition capabilities, have been implicated in the innate host defence Use: F, IF, IP TfR2 shares 45% identity with TfR1. In contrast to TfR1, expression of to pathogens. to pathogens. Quantity: 1 ml TfR2 is not downregulated as a result of iron overload, consistent with As investigated so far all functional characterized TLR signal via the As investigated so far all functional characterized TLR signal via the the absence iron-responsive element in TfR2. It is alternatively spliced TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems TLR/IL-1 receptor (IL-1R) pathway where recruitment of MyD88 seems to alpha and beta isoforms. TfR2 expression is controlled at the to be essential. to be essential. In contrast to cell-wall components, bacterial DNA is Transferrin Receptor, CD71 transcriptional level by the erythroid transcription factor GATA-1. In contrast to cell-wall components, bacterial DNA is probably invisible probably invisible for immune cells until DNA is liberated during for immune cells until DNA is liberated during processes taking place in processes taking place in the endosomal/lysosomal compartment Use: F, C, WB, IP, E the endosomal/lysosomal compartment where intracellular TLR9 recruits where intracellular TLR9 recruits MyD88 to initiate signal transduction. Cat.no. MON 1055 (Monoclonal) Quantity: 100 µg MyD88 to initiate signal transduction. Unmethylated CpG-dinucleotide- Unmethylated CpG-dinucleotide-containing sequences are found much containing sequences are found much more frequently in bacterial more frequently in bacterial genomes than in vertebrates genomes, Clone BL-C/A12 genomes than in vertebrates genomes, whereas the frequency of CpG whereas the frequency of CpG dinucleotides are suppressed and usually The antibody reacts with CD71, the transferrin receptor (a disulphide- Tumor Necrosis Factor-Receptor I dinucleotides are suppressed and usually methylated. The regions methylated. The regions adjacent to the CpG dinucleotides also affect bound homodimeric glycoprotein of 180 kD). The transferrin receptor adjacent to the CpG dinucleotides also affect the immunostimulatory the immunostimulatory activity. The optimal sequence differs is present on proliferating cells, including neoplastic cells, hemopoietic (p55/p60), CD120a activity. The optimal sequence differs significantly between mammalian significantly between mammalian species. Methylated CpG dinucleotides cells and lymphocytes. In tissue sections the antibody reacts also with species. Methylated CpG dinucleotides lack immunostimulatory lack immunostimulatory activities. Cellular activation in response to tissue macrophages. Cat.no. MON 9058 (Monoclonal) activities. Cellular activation in response to bacterial DNA and synthetic bacterial DNA and synthetic dinucleotides containing unmethylated dinucleotides containing unmethylated CpG-dinucleotides is mediated CpG-dinucleotides is mediated by TLR9. Use: C, F Clone H398 by TLR9. Quantity: 1 ml The antibody reacts with the extra-cellular part of the TNF-RI. It also Use: C, WB, E, F reacts with the soluble receptor. TNF-RI is present on most cell types Use: C, WB, E, F Quantity: 100 µg and is considered to play a prominent role in cell stimulation by TNF-α. Quantity: 100 µg Also available as BIOTIN conjugate (MON2093B - 50 µg) Transferrin Receptor, CD71 Induction of cytotoxicity and other functions are mediated largely via Also available as BIOTIN conjugate (MON2068B - 50 µg) TNF-RI.

Transferrin Receptor, CD71 Cat.no. MON 1140 (Monoclonal) Use: C, F, IP, WB Quantity: 100 µg Clone MEM-75 Also available as BIOTIN conjugate (MON 9058B - 50 µg) Cat. no. MON 3086 (Monoclonal) NEW The antibody reacts with the human CD71 antigen, the transferrin- receptor (a disulfide linked homodimer of 90 kD glycoprotein). The Clone 3B8 2A1 antigen is absent on resting B- and T-lymphocytes whereas it is expressed Tumor Necrosis Factor-Receptor I Human transferrin receptor 1 (TfR1), also designated CD71, is a on activated lymphocytes. It is also present on a wide range of other homodimeric type II membrane glycoprotein of 90-95 kDa. This receptor proliferating cells including neoplastic cells. It does not block binding (p55/p60), CD120a, Mouse binds two molecules of the serum iron-transport protein transferrin of transferrin to the receptor. (Tf) and is internalised into endosomes that are acidified, resulting in Cat.no. MON 9064 (Monoclonal) the release of iron from Tf. TfR1 is not expressed on resting leukocytes Use: C, IP but is upregulated on all proliferating cells upon activation, reflecting Quantity: 100 µg Clone HM104 the iron dependence of proliferation. In tissues TfR1 is expressed on The antibody reacts with the extra cellular part of the mouse TNF- most dividing cells and on brain capillary endothelium. Expression of Receptor p55 and with the soluble receptor. TNF-Receptor p55 is TfR1 is down regulated as a result of iron overload. TfR1 shares 45% present on most cell types and is considered to play a prominent role identity with TfR2. in cell stimulation by TNF-α. Induction of cytotoxicity and other most functions are mediated largely via TNF-Receptor p55. Use: F, C, WB, IP, E Quantity: 100 µg Use: E, F, IP, C Quantity: 100 µg

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Tumor Necrosis Factor-Receptor I Tumor Necrosis Factor-Receptor II Tumor Necrosis Factor-Receptor II Vitronectin Receptor alpha chain, CD51 (p55/p60), CD120a (p75/p80), CD120b (p75/p80), CD120b, Mouse Cat.no. MON 1130 (Monoclonal) Cat.no. MON 9062 (Monoclonal) Cat.no. MON 9063 (Monoclonal) Cat.no. PS 056 (Polyclonal) Clone CLB-706 Clone MR1-2 Clone MR2-1 The antibody reacts with the extra-cellular part of the mouse TNF-R II The antibody is directed against the CD51 antigen, alpha chain of The antibody reacts with the extra-cellular part of the TNF-R I. It also The antibody reacts with the extra-cellular part of the TNF-RII. It also and with the soluble receptor. TNF-R II is present on most cell types and vitronectin receptor (VNR-alpha chain) which forms a complex with: reacts with the soluble receptor. TNF-R I is present on most cell types and reacts with the soluble receptor. TNF-RII is present on most cell types and is considered to play a role in cell stimulation by TNF-alpha.. TNF-R II - the CD61 antigen (GP IIIa or Beta-3 chain) resulting in the alpha- is considered to play a prominent role in cell stimulation by TNF-alpha: is considered to play a prominent role in cell stimulation by TNF-alpha. molecule is shown to be responsible for stimulation of activated beta-3 complex, which is expressed on human platelets; Induction of cytotoxicity and other functions are mediated largely via TNF-RII molecule is shown to be responsible for stimulation of T-lymphocytes by TNF-alpha. Minimal cross reactivity with mouse - the beta-5 integrin, resulting in the alpha-beta-5 complex, which is TNF-R I. The antibody cross reacts with rhesus and cynomolgus natural activated T-lymphocytes by TNF-alpha. The antibody cross reacts with TNF-alpha. expressed on human monocytes and macrophages; TNF-R I. rhesus and cynomolgus natural TNF-RII. - the beta-1 integrin, resulting in the alpha-beta-1 complex. Use: E, WB, IP Use: C, F, E, WB, IP Use: C, F, E, WB, IP Quantity: 100 µg The platelet alpha-beta-3 complex functions as a vitronectin receptor Quantity: 100 µg Quantity: 100 µg and platelet adhesion to vitronectin is inhibited by this monoclonal Also available as BIOTIN conjugate (MON 9062B - 0.5 ml) Also available as BIOTIN conjugate (MON 9063B - 0.5 ml) antibody. Immunogen: human melanoma cells. Tumor Necrosis Factor-Receptor II Use: FS (p75/p80), CD120b Tumor Necrosis Factor-Receptor I Tumor Necrosis Factor-Receptor II Quantity: 200 µg (p55/p60), CD120a (p75/p80), CD120b, Mouse Cat.no. MON 9059 (Monoclonal) Vitronectin Receptor beta chain, Cat.no. PS 053 (Polyclonal) Cat.no. MON 9065 (Monoclonal) Clone 80M2 The antibody reacts with the extra-cellular part of the TNF-RII. It also Platelet GPIIIa, CD61 The antibody reacts with the extra-cellular part of the TNF-R I and with Clone HM102 reacts with the soluble receptor. TNF-RII is present on most cell types the soluble receptor. TNF-R I is present on most cell types and is The antibody reacts with the extra-cellular part of the mouse TNF-RII and is considered to be an important co-stimulator together with TNF-RI Cat.no. MON 1051 (Monoclonal) considered to play a prominent role in cell stimulation by TNF-alpha. and with the soluble receptor. TNF-RII is present on most cell types and in cellular responses to TNF-alpha, in particular the transmembrane form Induction of cytotoxicity and other functions are mediated largely via is considered to play a prominent role in cell stimulation by TNF-alpha. of TNF-alpha. A dominant role of TNF-RII has been shown in thymocyte Clone BL-E6 TNF-R I. The TNF-RII molecule is shown to be responsible for stimulation of activation by TNF-alpha. The anti CD61 antibody recognizes the platelet glycoprotein gpIIIa/VNR activated T-lymphocytes by TNF-alpha. β-chain. This glycoprotein is the common β-chain ( β3-Integrin) of the Use: E, WB, IP Use: C, F, IP, WB, E cytoadhesins, gpIIIbIIIa and VNR. The antibody reacts with thrombocytes Quantity: 100 µg Use: E, F, IP, C Quantity: 100 µg and megakaryocytes. Quantity: 100 µg Also available as BIOTIN conjugate (MON 9059B - 50 µg) Use: C, F Tumor Necrosis Factor-Receptor I Quantity: 1 ml (p55/p60), CD120a, Mouse Tumor Necrosis Factor-Receptor II Vitronectin Receptor alpha chain, (p75/p80), CD120b CD51/CD61 Vitronectin Receptor beta chain, Cat.no. PS 055 (Polyclonal) Cat.no. PS 054 (Polyclonal) Cat.no. MON 1167 (Monoclonal) Platelet GPIIIa, CD61 The antibody reacts with the extra-cellular part of the mouse TNF-R I and with the soluble receptor. TNF-R I is present on most cell types and The antibody reacts with the extra-cellular part of the TNF-R II and Clone 23C6 Cat.no. MON 1147 (Monoclonal) is considered to play a prominent role in cell stimulation by TNF-alpha. with the soluble receptor. TNF-R II is present on most cell types and 23C6 was assigned to the CD51 cluster at the 4th International Induction of cytotoxicity and other functions are mediated largely via is considered to play a role in cell stimulation by TNF-alpha. TNF-R II Workshop Vienna 1989. This antibody recognizes a 110/90 kDa antigen Clone CRC54 TNF-R I. A minor cross-reactivity with mouse TNF-alpha is molecule is shown to be responsible for stimulation of activated known as the α-chain of the vitronectin receptor (VNR). 23C6 reacts The anti CD61 antibody reacts with the platelet glycoprotein gpIIIa/VNR detectable. T-lymphocytes by TNF-alpha. with osteoclasts, placenta, melanoma cell lines, some epithelia cells, and β-chain. This glycoprotein is the common β-chain (β3-Integrin) of the reacts weakly with platelets. Note: 23C6 shows no reactivity on fixed cytoadhesins, gpIIbIIIa and VNR. The antibody reacts with platelets and Use: E, WB, IP, C Use: E, WB, IP, C tissues. The vitronectin receptor mediates cell adhesion to arg-gly-arp megakaryocytes. The antibody reacts preferentially with the activated/ Quantity: 100 µg Quantity: 100 µg containing sequences in vitronectin, von Willebrand factor, fibrinogen, occupied form of platelet GPIIb-IIIa (ligand- induced biding site or LIBS and thrombospondin. Used antigen: Human Osteclastoma tissue. Cross epitope) and dissociated form of GPIIIa. CRC54-epitope is located reactive with rabbit vitronectin receptor. Clinical relevance: Bone within the N-terminal region of GPIIIa (first 100 amino acids). CRC54 resorption modulation; osteoclast identification, receptor purification; does not react with platelets of patients with Glanzmann thrombastenia. malignant melanoma identification. Use: C, F, IF Use: C, F, IP Quantity: 1 ml Quantity: 200 µg

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Blood group antigen A Blood group antigen H (H2, Ley, Leb) Erythrocytes Glycophorin

Cat.no. MON 2044 (Monoclonal) Cat.no. MON 2046 (Monoclonal) Cat.no. MON 7050 (Monoclonal) Cat.no. MON 7045 (Monoclonal)

Clone HE-193 Clone A70-A/A9 Clone SFL23.6 Clone A63-B/C2 This antibody reacts with human blood group A antigen type 1, type 2 A70-A/A9 recognizes the epitope Fucα1-2Galβ1 and is therefore specific SFL23.6 is a mouse IgG2b monoclonal antibody directed against an The mAb A63-B/C2 reacts with a peptide epitope on the extra cellular (mono-and difucosyl), type 3, type 4, type 5, type 6, Forsmann. for blood group antigen H detecting H2, Ley and Leb and the mAb has erythroid cell surface antigen. The antibody was generated from mice domain of human glycophorin. There is no cross reactivity with bovine no cross reactivities with the blood group antigens A, B, Lea and Lex. hyper immunized with liver cells from 20-22 week-old human fetuses. glycophorin. Source: A BALB/c mouse was immunized with human Use: P The specificity of the mAb closely resembles that of mAb M/A 15-5 SFL23.6 shows a well defined reactivity with cells of the erythroid lineage erythrocytes, treated with neuraminidase (V. cholerae). Splenocytes were Quantity: 1 ml (Miyake & Hakomori, Biochem. 30, 3328 (1991). Source: BALB/c mice at all stages of maturation in the peripheral blood, bone marrow, and fused with X63-Ag8.653 cells. were immunized with neuraminidase-treated live cells of the T-47D fetal liver. Non-erythroid lineages are negative by flow cytometric breast carcinoma cell lines. The isolated splenocytes of the mice were analyses. Although the exact identity of the antigen has not yet been Use: F, A Blood group antigen A fused with the myeloma cell line X63-Ag8.653. determined, it has been shown to be distinct from glycophorin A. The Quantity: 100 µg antibody is unreactive in B5 or formalin fixed paraffin embedded sections Use: F, P but is reactive with unfixed cells or paraformaldehyde fixed cell Cat.no. MON 2045 (Monoclonal) Quantity: 100 µg preparations. Glycophorin Clone HE-195 Use: C This antibody reacts with human blood group A, including weak variants Blood group Lewisy Quantity: 100 µg Cat.no. MON 7046 (Monoclonal) A3, Ax, A3B, AxB. Clone A84-B/H2 Use: A Cat.no. MON 2047 (Monoclonal) Fibrin/Fibrinogen A84-B/H2 reacts with human glycophorin. Source: A BALB/c mouse Quantity: 1 ml was immunized with human erythrocytes, treated with neuraminidase Clone A70-C/C8 (V. cholerae). Splenocytes were fused with X63-Ag8.653 cells. A70-C/C8 recognize the epitope Fucα1-2Galβ1-4(Fucα1-3)GlcNAcβ1 Cat.no. MON 5019 (Monoclonal) Blood group antigen A1B and is therefore specific for blood group antigen Lewisy. The mAb has Use: F, E no cross reactivities with the blood group H type 2 antigens or with Lea, Clone MFB-HB Quantity: 100 µg sialyl-Lea, Lex, sialyl-Lex,Leb or Lec. Source: A NZB/N Bom mouse was The antibody reacts specifically with fibrin of human and canine origin. Cat.no. MON 2041 (Monoclonal) immunized with live cells of the human colorectal carcinoma cell line It binds with moderate affinity to human fibrinogen, but does not react Ls174T. The isolated splenocytes of the mouse were fused with the with other human plasma proteins. Leukemia cell line HL-60 Clone HE-24 myeloma cell line X63-Ag8.653. This antibody reacts with human blood group A1B antigens. Use: IF, E, F Use: F, P Quantity: 500 µg Cat.no. MON 8035 (Monoclonal) Use: P, A Quantity: 100 µg Quantity: 1 ml Clone IPO-M6 Fibronectin, cellular, plasma IPO-M6 reacts with human leukemia cell line HL-60 and immuno Endothelial Cell Protein C Receptor (EPCR) precipitates with two proteins with MW of 48 and 52 kDa. IPO-M6 Blood group antigen ABH does not stain B-cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell Cat.no. MON 5064 (Monoclonal) lines CCRF-HSB2, Jurkat and Molt-4. Source: IPO-M6 was generated Cat. no. MON 9055 (Monoclonal) NEW after immunization of a BALB/c mouse with human cell line HL-60. Cat.no. MON 2040 (Monoclonal) Clone Fn-4 Mouse splenocytes were fused with the mouse myeloma cell line Clone LMR-42 The antibody reacts with the human cellular and plasma fibronectin. P3-X63-Ag8.653. Clone HE-10 The rat monoclonal antibody LMR-42 detects an outer-surface epitope This antibody reacts with human blood group A, B, H type 3, 4, 5 antigens. of a 55 kDa plasma membrane protein overexpressed in several human Use: IF, IP Use: F, C non-Pgpmultidrug-resistant (MDR) tumor cell lines. Expression cloning Quantity: 100 µg Quantity: 100 µg Use: P, A revealed that the LMR-42 antigen is identical to the human endothelial Quantity: 1 ml cell protein Creceptor (EPCR). Protein C is the zymogen of the key anticoagulant enzyme, activated protein C (APC). Lewisa Blood group antigen B Use: F, C Quantity: 100 µg Cat.no. MON 3051 (Monoclonal)

Cat.no. MON 2042 (Monoclonal) Clone 7LE 7LE reacts with Lewisa antigen. Source: A BALB/c mouse was immunized Clone HEB-29 with mucin, isolated from an ovarian cyst fluid (pure endocervical type This antibody reacts with human blood group B antigens. according to the Fenoglio’s classification). Splenocytes were fused with mouse myeloma SP2/0 cells. Use: P, A Quantity: 1 ml Use: F, P, E Quantity: 100 µg

96 For research use only. Not for drug, diagnostic or other use. www.monosan.com 97 Haematology Hormones

Sulfo-Lewisa, MUC5B ACTH Angiotensin I

Cat.no. MON 1154 (Monoclonal) Cat.no. MON 3071 (Monoclonal) Cat.no. PS 073 (Polyclonal)

Clone F2 Clone 2F6 Human Angiotensin (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu) synthesed The antibody F2 reacts in ELISA with the synthetically prepared sulfo- The mAb 2F6 demonstrates broad species cross reactivity. It localizes and purified to 98% is linked to bovine serum albumin by means of Lewisa antigen and with the subpopulation of MUC5B (specific for ACTH in immunohistochemical techniques on formalin-fixed, paraffin- carbodiimide in random orientation. This conjugate is used to immunize MUC5B), but not with Lewisa or sulfo-Lewisx antigens. The antibody embedded or frozen sections. Source: A BALB/c mouse was immunized rabbit. Pooled antisera are passed over DEAE-cellulose to produce

binds to isolated high Mr mucins, the epitope was mapped to the with ACTH synthetic peptide (1-24 fragment) conjugated KLH. IgG-enriched fraction, which is further subjected to absorption with a SO3- 3Galβ1-3GlcNac-moiety of the Sulfo-Le antigen. The F2 antibody Splenocytes were fused with SP2/0. immobilized human albumin and immmunoglobuline fractions in order

binds to high Mr salivary mucins, colon mucins, gallbladder mucins and to remove non-specific antibodies. Specificity of the antisera is confirmed

cervical mucins, but not to low Mr salivery mucins and gastric mucins. Use: F, P, E by its ability to bind and precipitate labelled angiotensin I and to be In immunohistological sections a positive reaction was found with a Quantity: 100 µg adsorbed by angiotensin. Native angiotensin competes with such number of epithelia, including the (sero)mucous salivary glands, the binding, while human whole plasma or plasma proteins do not. The goblet cells of the colon, the lining epithelium of cervical and antiserum also reacts with angiotensin II. esophageal glands, the suprabasal keratinocytes and Hassall’s corpuscles ACTH of the thymus. In addition, positive reaction in flow cytometry was Use: E found with an ovarian carnicoma cell line. No staining was found in Quantity: 1 ml normal breast, pancreas, small intestine, spleen and lymph nodes. Cat.no. PS 080 (Polyclonal) Normal gastric glands were negative, but gastric intestinal metaplastic glands were strongly stained with F2 antibody. ACTH can be used to determine specific hormone production by pituitary Bombesin In rat tissues, the F2 antibody labeled epithelial cells of the salivary adenomas. A further use is in the investigation of ectopic hormone glands, colon and stomach. In addition to epithelial cells, extracellular production by malignant tumours; The most common ACTH producing matrix components in rat thymus and skin were labeled by the F2 tumour is bronchogenic carcinoma. The immunogen is: synthetic ACTH. Cat.no. PS089 (Polyclonal) antibody. No labeling of erythrocytes, lymphocytes or bone marrow cells Antigen localisation: cytoplasm. Cross reactivity with human, rat, mouse was found by flow cytometry. and pig. This Bombesin antibody can be used for the study of endocrine The present data shows a tissue specific distribution of the F2-epitope differentiation in tumours of the respiratory tract. Antigen localization: in cells from the epithelial lineage in human and rat. Use: F, P, E Cytoplasm. Immunogen: synthetic frog bombesin conjugated to bovine Quantity: 0.25 ml Thyroglobulin. Cross reactive with human, rat and mouse bombesin. Use: IF, C, WB, E, P Quantity: 1 ml Use: F, P Adrenomedullin 1-6, Rat Quantity: 0.25 ml LewisB Cat.no. PS 255 (Polyclonal) Bombesin/GRP Cat.no. MON 3047 (Monoclonal) Immunogen: Synthetic rat adrenomedullin 1-6, conjugated to BSA. Tested on rat tissues. Cat.no. PS223 (Polyclonal) Clone 2-25LE Positive control: Stefanini-fixed frozen sections of fundus from rat. 2-25LE reacts with Lewisb antigen. Source: A BALB/c mouse was Immunogen: Bombesin, conjugated to BSA. Tested on human, rat and immunized with mucin, isolated from an ovarian cyst fluid Use: F chicken tissues. Positive control: formalin-fixed paraffin sections of rat (pure endocervical type according to the Fenoglio’s classification). Quantity: 50 µl or chicken stomach. Absorption with 10-100 µg bombesin and GRP per Splenocytes were fused with mouse myeloma SP2/0 cells. ml diluted antiserum abolishes the staining.

Use: F, P, E Amylin Use: P, F Quantity: 100 µg Quantity: 50 µl

Cat.no. PS214 (Polyclonal) Vitronectin Immunogen: Amylin. Tested on human, rat, cat, pig and guinea-pig tissues. Positive control: formalin-fixed paraffin and frozen sections of Cat.no. MON 3050 (Monoclonal) human or rat pancreas. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining. Clone BV1 This monoclonal antibody (subclass IgG1) binds to human vitronectin. Use: P, F It binds to soluble vitronectin as well as to membrane bound Quantity: 50 µl vitronectin.

Use: WB, IF, IP Quantity: 100 µg

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Calcitonin CGRP Chorionic gonadotropin (hCG) Enkephalin

Cat.no. PS081 (Polyclonal) Cat.no. PS 252 (Polyclonal) Cat.no. MON 3062 (Monoclonal) Cat.no. PS 202 (Polyclonal)

Calcitonin is produced by the C-cells of the thyroid. This antibody can Immunogen: Synthetic human calcitonin generelated peptide. Tested Clone HCG-55 Synthetic met-enkephalin conjugated to BSA. Tested on cat and pig be used in the diagnosis of medullary thyroid carcinoma and of C-cell on human and rat tissues. Positive control: Stefanini fixed frozen The antibody reacts with β-subunit of human chorionic gonadotropin. tissues. Positive control: Frozen sections of cat or pig small intestine. hyperplasia. Calcitonin may also occur in bronchial carcinoids and in sections of rat colon. Absorption with 10-100 µg CGRP per ml diluted Cross reactivity in % with hTSH (<0.5), hFSH (<0.5), hLH (<1.55), Absorption with 10-100 µg immunogen per ml diluted antiserum tumours of adrenal medulla and of pancreatic islets. antiserum abolishes the staining, while calcitonin does not. Cross reacts β-hCG (86). The cross reactivity with other glycoprotein hormones and abolishes the staining, while B-endorphin does not Cross-reacts with Immunogen: synthetic human Calcitonin. Antigen location, cytoplasm. with amylin (IAPP). free subunits of HCG was estimated in double-antibody RIA. Affinity leu-enkephalin. Crossreactive with calcitonin in most species. constant: 4.0 x 10E10 M-1. Use: P, F Use: P, F Use: F, P, IB Quantity: 50 µl Use: IRMA, IEMA, ILMA, E, IC Quantity: 50 ml Quantity: 0.25 ml Quantity: 100 µg

CGRP Follicle Stimulating Hormone, FSH CGRP Chorionic gonadotropin (hCG) Cat.no. PS 254 (Polyclonal) Cat.no. MON 5012 (Monoclonal) Cat.no. PS 079 (Polyclonal) Cat.no. MON 3063 (Monoclonal) Immunogen: Calcitonin generelated peptide (synthetic, rat), conjugated Clone 1038 CGRP is present in C-cells of the thyroid and in central and peripheral to BSA. Tested on human, mouse and rat tissues. Positive control: Clone HCG-60 The antibody is specific for FSH. This was ascertained by ELISA and nerves. The antibody can be used for the tests on Medullary thyroid Stefanini-fixed frozen sections of rat colon. Absorption with 10-100 µg The antibody reacts with β-subunit of human chorionic gonadotropin. immunohistochemistry. Follicle stimulating hormone is produced in carcinomas. The immunogen is: synthetic rat CGRP. Antigen localization: CGRP per ml diluted antiserum abolishes the staining, while calcitonin Cross reactivity in % with hTSH (<0.5), hFSH (<0.5), hLH (<1.55), the anterior lobe of the pituitary gland. cytoplasm, extra cellular. Cross reactivity (also with amylin) with human does not. Does not cross react with amylin (IAPP). β-hCG (86). The cross reactivity with other glycoprotein hormones and and rat. free subunits of HCG was estimated in double-antibody RIA. Affinity Use: F, P Use: P, F constant: 8.9 x 10E10 M-1. Quantity: 1 ml Use: F, P Quantity: 50 µl Quantity: 0.25 ml Use: IRMA, IEMA, ILMA, E, IC Quantity: 100 µg Gastrin Chorionic gonadotropin (hCG), beta CGRP Chorionic gonadotropin (hCG) Cat.no. PS 085 (Polyclonal) Cat.no. MON 3067 (Monoclonal) Cat.no. PS 226 (Polyclonal) Gastrin secreting cells are numerous in the antrum and few are found Clone 5H4-E2 Cat.no. MON 3064 (Monoclonal) in the proximal duodenum. The antibody can be used for the diagnosis Immunogen: Calcitonin generelated peptide (synthetic, rat) conjugated 5H4-E2 is specific for the Beta-chain of hCG. Since the Beta-chain levels of gastrin producing tumours which are most found in the pancreas to BSA. Tested on human and rat tissues. Positive control: Stefanini-fixed rise faster in urine, the antibody will detect hCG in urine as early as Clone HCG-61 and occasionally in the stomach and duodenum. Immunogen: human frozen sections of rat colon. Absorption with 10-100 µg CGRP per ml 1-3 days after successful (artificial) conception as opposed to alpha or The antibody reacts with β-subunit of human chorionic gonadotropin. gastrin 17 (Sigma, G3141) conjugated to BSA. Antigen location, diluted antiserum abolishes the staining while calcitonin, SP and NKA dimer specific antibodies. In hCG producing tumours such as certain Cross reactivity in % with hTSH (<0.5), hFSH (<0.5), hLH (<0.86), cytoplasm, extra cellular. Cross reactive with gastrin in most mammals. do not. Cross reacts with amylin (IAPP). chorial, testicular, embyionic and lung carcinomas, the Beta-chain is β-hCG (77). The cross reactivity with other glycoprotein hormones and also produced in higher quantities than the alpha or dimeric chains. free subunits of HCG was estimated in double-antibody RIA. Affinity Use: F, P, WB Use: P, F Source: A BALB/c mouse was immunized with Beta-hCG, conjugated to constant: 5.1 x 10E10 M-1. Quantity: 0.25 ml Quantity: 50 µl various carrier proteins. Splenocytes were fused with SP2/0-Ag14 cells. Use: IRMA, IEMA, ILMA, E, IC Use: F, P, E Quantity: 100 µg Gastrin, Rat CGRP, Rat Quantity: 100 µg Chorionic gonadotropin, b-HCG Cat.no. PS 250 (Polyclonal) Cat.no. PS 239 (Polyclonal) Immunogen: Synthetic human gastrin I, conjugated to BSA. Tested on Immunogen: CGRP, rat, conjugated to BSA. Tested on rat and mouse Cat.no. MON 2001 (Monoclonal) rat tissues. tissues. Positive control: Stefanini-fixed frozen sections of rat colon. Positive control: formalin-fixed paraffin sections of rat antrum. Absorption with 10-100 µg CGRP per ml diluted antiserum abolishes the Clone 2092 Absorption with 10-100 µg gastrin 1-34 and CCK 8 per ml diluted staining, while calcitonin does not. Does not cross react with amylin The antibody reacts with high with β-HCG. antiserum abolishes the staining. (IAPP). Use: F, P, IF, E Use: P, F Use: P, F Quantity: 1 ml (MON 2001-1) Quantity: 50 µl Quantity: 50 µl 5 ml (MON 2001-5)

100 For research use only. Not for drug, diagnostic or other use. www.monosan.com 101 Hormones Hormones

Gastrin/CCK Glucagon Helospectin, Mouse Insulin

Cat.no. PS220 (Polyclonal) Cat.no. PS 215 (Polyclonal) Cat.no. PS 238 (Polyclonal) Cat.no. MON 5088 (Monoclonal)

Immunogen: Synthetic human gastrin 1-34. Tested on human, rat, cat Immunogen: Porcine pancreatic glucagon, conjugated to HSA. Tested Immunogen: Synthetic helospectin I. Tested on rat and mouse tissues. Clone E2-E3 and pig tissues. Positive control: formalin-fixed paraffin sections of rat on human, pig and rat tissues. Positive control: frozen sections of pig Positive control: Stefanini-fixed frozen sections of rat small intestine. Insulin is a protein consisting of an α-chain of 21 amino acids and a antrum. Absorption with 10-100 µg gastrin 1-34, gastrin 1-17, CCK 8 or rat pancreas. Absorption with 10-100 µg immunogen per ml diluted (1% carbodiimide added to the fixative). Absorption with 10-100 µg β-chain of 30 amino acids and produced in the β-cells of the pancreas. and CCK 4 per ml diluted antiserum abolishes the staining, while CCK antiserum abolishes the staining, while glicentin, secretin, GIP and VIP helospectin per ml diluted antiserum abolishes the staining, while E2-E3 is a specific insulin antibody as tested by ELISA and on human 10-20 does not. do not. secretin, PHI and PACAP do not. Cross reacts with helodermin. pancreatic tissue. Source: A BALB/c mouse was immunized with swine insulin. The mouse spleen cells were fused with SP2/0 Ag14 cells and Use: P, F Use: P, F Use: F the clones were selected on anti-insulin activity. Quantity: 50 µl Quantity: 50 µl Quantity: 50 µl Use: P, E Quantity: 100 µg Glicentin Glucagon Histamine Insulin Cat.no. PS086 (Polyclonal) Cat.no. PS 248 (Polyclonal) Cat.no. PS 242 (Polyclonal)

Glicentin contains the glucagon sequence and is produced in endocrine Immunogen: Highly purified porcine pancreatic glucagon. Tested on Immunogen: Histamine conjugated to HSA. Tested on rat tissues. Cat.no. MON 5023 (Monoclonal) cells of the distal intestine, in pancreatic glucagon cells and in nerves human and cat tissues. Positive control: Bouin-fixed paraffin sections Positive control: Cryostat sections of carbodiimide fixed human skin or in the brain. This glicentin antibody is reactive with glicentin as well as of cat pancreas. Absorption with 10-100 µg glucagon per ml diluted freeze-dried paraffin sections (vapour fixed in DEPC) of rat stomach Clone IN-05 glucagon. The antibody can be used for the diagnosis of tumours from antiserum abolishes the staining. (see ref.). Absorption with 10-100 µg histamine per ml diluted antiserum The antibody is specific for human insulin. It recognizes also porcine and the distal intestin (rectal carcinoids) as well as pancreatic islet cell abolishes the staining, while noradrenaline, 5-HT, VIP, glucagon and bovine insulin. No cross reactions were found with other serum proteins. tumours. Immunogen: porcine pancreatic Glucagon/BSA. Antigen Use: P, F histidine do not. location, cytoplasm. Cross reactive with human and rat Glicentin. Quantity: 50 µl Use: E, RIA, P Use: P, F Quantity: 100 µg Use: F, P Quantity: 50 µl Quantity: 0.25 ml Growth hormone, (hGH) Insulin Insulin Glucagon/Glicentin, Porcine Cat.no. MON 3061 (Monoclonal) Cat.no. PS088 (Polyclonal) Clone GH-45 Cat.no. MON 5094 (Monoclonal) Cat.no. PS221 (Polyclonal) The antibody reacts with human growth hormone, it does not bind Insulin is produced by the B-cells of the pancreatic islets. This insulin human prolactin or any other pituitary hormones. Affinity constant: Clone 2D11-H5 antibody can be used for the diagnosis of pancreatic B-cell tumours. Immunogen: Porcine pancreatic glucagon, conjugated to HSA. Tested 3.82 x 10E9 M-1. Insulin is a protein consisting of an α-chain of 21 amino acids and a Immunogen: porcine insulin. Antigen location, cytoplasm, extra cellular. on pig tissues. Positive control: formalin-fixed paraffin sections of pig β-chain of 30 amino acids and produced in the β-cells of the pancreas. Cross reactive with human, pig and rat. pancreas. Frozen sections of rat pancreas. Absorption with 10-100 µg Use: P, IC 2D11-H5 is a specific insulin mAb as tested by ELISA and on human glucagon and glicentin per ml diluted antiserum abolishes the staining, Quantity: 100 µg pancreatic tissue. Source: A BALB/c mouse was immunized with swine Use: F, P, E while secretin, GIP and VIP do not. insulin. The mouse spleen cells were fused with SP2/0 Ag14 cells and Quantity: 0.25 ml the clones were selected on anti-insulin activity. Use: P, F Growth hormone, (hGH) Quantity: 50 µl Use: P, E Insulin Quantity: 100 µg Cat.no. PS036 (Polyclonal) Glucagon Cat.no. PS222 (Polyclonal) The antiserum reacts specifically with human and pig growth hormone. Immunogen: Porcine insulin. Tested on human, rat and pig tissues. Cat.no. PS087 (Polyclonal) Use: F, P Positive control: formalin-fixed paraffin or frozen sections of rat pancreas. Quantity: 0.25 ml No cross reactions with polypeptide hormones unrelated to insulin. Glucagon is produced by the alpha-cells of the pancreatic islets. It occurs also in endocrine cells of the gut and in neurons in the brain. The Use: P, F glucagon antibody can be used for demonstration of pancreatic Quantity: 50 µl endocrine tumours. Antigen localization: cytoplasm. Immunogen: porcine pancreatic Glucagon/BSA. Cross reactive with human and rat.

Use: F, P Quantity: 0.25 ml

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Insulin/Proinsulin Oxytocin Pancreatic Polypeptide Progesterone

Cat.no. PS 240 (Polyclonal) Cat.no. PS 207 (Polyclonal) Cat.no. PS 216 (Polyclonal) Cat.no. MON 2050 (Monoclonal)

Immunogen: Synthetic human proinsulin. Tested on human and pig Oxytocin conjugated to bovine thyroglobuline. Tested on pig tissues. Immunogen: Pure bovine pancreatic polypeptide. Tested on human and Clone Pg.53 tissues. Positive control: formalin-fixed paraffin sections of human Positive control: Stefanini-fixed frozen sections of pituitary of pig. pig tissues. Positive control: formalin-fixed paraffin sections of pig This antibody reacts with human progesterone. pancreas. Absorption with 10-100 µg proinsulin per ml diluted antiserum Absorption with 10-100 µg immunogen per ml diluted antiserum duodenal pancreas. Frozen sections of rat pancreas. Absorption with inactivates the antiserum, while C-peptide and insulin only partly abolishes the staining. 10-100 µg immunogen per ml diluted antiserum abolishes the staining, Use: E reduces staining. while porcine PYY, NPY or glucagon do not. Quantity: 100 µg Use: F Use: P, F Quantity: 50 µl Use: P, F Quantity: 50 µl Quantity: 50 µl Secretin PACAP 38 LH-RH Pancreastatin Cat.no. PS 092 (Polyclonal) Cat.no. PS 234 (Polyclonal) Secretin producing cells are most frequently found in the proximal part Cat.no. PS 082 (Polyclonal) Cat.no. PS 205 (Polyclonal) of the duodenum to a lesser extend in the jejunum. The antibody can Immunogen: Synthetic PACAP38 conjugated to BSA. Tested on rat and be used for the diagnosis of tumours derived from these cells. The Luteinizing Hormone Releasing Hormone (LH-RH) is produced in human tissues. Positive control: Stefanini-fixed frozen sections of rat Synthetic human pancreastatin 32-49, conjugated to BSA. Tested on Immunogen: Porcine secretin. Antigen location, cyotoplasm. Cross the hypothalamus and influences the release of Luteinizing Hormone duodenum and spinal cord. Absorption with 10-100 µg immunogen human tissues. Positive control: frozen sections of human pancreas. reactive with human and pig. in the pituitary gland. LH-RH is also known as Gonadotropin-releasing per ml diluted antiserum abolishes the staining, while PRP, VIP, PHI, CRF, Absorption with 10-100 µg immunogen per ml diluted antiserum hormone (Gn-RH). Antigen localization: cytoplasm. Immunogen: oxytocin and vasopressin do not. Some cross reactivity with PACAP27 abolishes the staining. Use: F, P Human LH-RH conjugated to BSA. Cross reactivity, not known. Quantity: 0.25 ml Use: F Use: P, F Use: F, P, E Quantity: 50 µl Quantity: 50 µl Quantity: 0.25 ml Secretin PACAP-related peptide (PRP), Rat Pancreastatin Motilin Cat.no. PS 217 (Polyclonal) Cat.no. PS 235 (Polyclonal) Cat.no. PS 241 (Polyclonal) Immunogen: Pure porcine secretin. Tested on human and pig tissues. Cat.no. PS 208 (Polyclonal) Positive control: frozen sections of rat duodenum. Absorption with Immunogen: Synthetic rat PRP conjugated to BSA. Tested on rat tissues. Immunogen: Synthetic chromogranin A (306-314), rat. Tested on rat 10-100 µg immunogen per ml diluted antiserum abolishes the staining, Pure porcine motilin, conjugated to BSA. Tested on human, pig and cat Does not work on human tissues. Positive control: Stefanini-fixed tissues. Positive control: Bouin-fixed paraffin sections of rat pancreas. while glucagon, GIP, PP and VIP do not. tissues. Does not work on rat tissues. Positive control: formalin-fixed frozen sections of rat duodenum, fundus or antrum. Absorption with Absorption with 10-100 µg immunogen per ml diluted antiserum paraffin sections of pig duodenum. Absorption with 10-100 µg 10-100 µg immunogen per ml diluted antiserum abolishes the staining, abolishes the staining. Use: P, F immunogen per ml diluted antiserum abolishes the staining. while PACAP27, PACAP38, VIP, PHI, CRF, oxytocin and vasopressin do not. Quantity: 50 µl Use: P, F Use: P, F Use: F Quantity: 50 µg Quantity: 50 µl Quantity: 50 µl Somatostatin Progesterone MSH (Alpha) Pancreas Polypeptide Cat.no. PS083 (Polyclonal) Cat.no. MON 2049 (Monoclonal) Somatostatin occurs in nerves in the brain and the gut and in endocrine Cat.no. PS 203 (Polyclonal) Cat.no. PS091 (Polyclonal) cells of the gut and pancreas. Therefore, this antibody can be used for Clone Pg.13 the differential diagnosis of tumours with this origin. Antigen localization: Synthetic α-MSH conjugated to BSA. Tested on pig and rat tissues. Pancreas polypeptide (PP) is located in the pancreatic islets as well as This antibody reacts with human progesterone. Cytoplasm. Immunogen: Human somatostatin (SRIF; UCB) conjugated Positive control: Bouin-fixed paraffin or Stefanini-fixed frozen sections in the exocrine pancreas. This PP antibody can be used for the diagnosis to ovalbumin. Cross reactive with rat and pig. of pig pituitary. Absorption with 10-100 µg immunogen per ml diluted of tumours of pancreatic origin and of bronchial and rectal carcinoids. Use: E antiserum abolishes the staining. Immunogen: bovine PP conjugated to BSA. Antigen location, cyotoplasm. Quantity: 100 µg Use: F, P, E, WB Cross reactive with human and pig. Quantity: 0.25 ml Use: P, F Quantity: 50 µl Use: F, P, E Quantity: 0.25 ml

104 For research use only. Not for drug, diagnostic or other use. www.monosan.com 105 Hormones Immunoglobulins

Somatostatin Thyroid stimulating hormone, (hTSH) Immunoglobulins IgE

Cat.no. PS204 (Polyclonal) Cat.no. MON 3066 (Monoclonal) (Monoclonal) Cat.no. MON5035 (Monoclonal) IgA, clone MH14/1 Cat.no. MON 5007 Cyclic somatostatin 14, conjugated to thyroglobulin. Tested on pig and Clone TSH-116 IgE, clone MH25/1 Cat.no. MON 5008 Clone BL-E9 human tissues. Positive control: formalin-fixed paraffin sections of pig The antibody reacts with hTSH. Cross reactivity in % with hLH (23.2), IgG, clone MH16/1 Cat.no. MON 5009 The antibody is highly specific for human IgE. The antibody can be used pancreas. Absorption with 10-100 µg immunogen per ml diluted hFSH (29.9), hCG (20.3), hTSH (78.9). The cross reactivity with other IgM, clone MH15/1 Cat.no. MON 5010 for studies on atopic reactions. It is useful for detection and quantification antiserum abolishes the staining. glycoprotein hormones and free subunits of hTSH was estimated in of specific IgE antibodies against allergens. double antibody RIA. Affinity constant: 1.1 x 10E11M-1. The monoclonals are highly specific for the individual Ig isotypes. Use: P, F Use: E Quantity: 50 µl Use: RIA, E Use: F, P, IF Quantity: 1 ml Quantity: 100 µg Quantity: 1 ml

Thyroglobulin, Tg IgE, Mouse IgA Cat.no. MON 5003 (Monoclonal) Cat.no. MON 5051 (Monoclonal) Cat.no. MON 5004 (Monoclonal) Clone 14/14 Clone LO-ME-3 The antibody reacts in immunoblot with 27S, 19S and 12S thyroglobulin Clone BFG-267 Mouse epsilon heavy chain of immunoglobulin (determined by and lacks reactivity with cyogen bromide cleavage products of This monoclonal antibody reacts with human IgA, α-chain. No cross immunodot). thyroglobulin. It cross reacts with thyroglobulin from several species reaction with human plasma proteins other then human IgA. No cross Avidity for IgE: 3 * 109 M-1 including rat, goat, chicken and mouse. It does not cross react with reaction with purified human IgE, IgG and IgM. Apparent binding different human tissues tested, including kidney, ovary, endometrium, constant is approximately 0.01 nM. Conjugation of the antibody with Use: E, F testis, salivary gland, colon, appendix, antrum, adrenal, liver, pancreas, peroxidase, FITC or biotin does not impair the binding properties. Quantity: 1 ml spleen, bronchus epithelium, duodenum, parathyroid and mammary Immunogen: purified α-chain (Fc) of human IgA. Specificity of the Also available as FITC conjugate (MON5051F - 1 ml) gland tissue. monoclonal was determined by ELISA on purified human immuno- Also available as HRP conjugate (MON5051P - 1 ml) globulin isotypes. Also available as BIOTIN conjugate (MON 5051B - 1 ml) Use: P, E Quantity: 1 ml (MON 5003-1) Use: E 5 ml (MON 5003-5) Quantity: 1 ml IgG, Chicken Also available as FITC conjugate (MON 5004F - 1 ml) Also available as HRP conjugate (MON 5004P - 1 ml) Thyroid stimulating hormone, (hTSH) Cat.no. MON 5045 (Monoclonal) IgA, Mouse Clone 409-3.1 Cat.no. MON 3065 (Monoclonal) The antibody reacts with anti Chicken IgG

Clone TSH-51 Cat.no. MON 5050 (Monoclonal) Use: E, IB The antibody reacts with hTSH. Cross reactivity in % with hLH (2.99), Quantity: 100 µg hFSH (0.66), hCG (0.03), hTSH (68.6). The cross reactivity with other Clone LO-MA-7 glycoprotein hormones and free subunits of hTSH was estimated in Mouse alpha heavy chain of immunoglobulin (determined by IgG double antibody RIA. Affinity constant: 5.5 x 10E10M-1. immunodot), avidity on IgA: 4.8 * 109 M-1.

Use: RIA, E Use: F Cat.no. MON 5014 (Monoclonal) Quantity: 100 µg Quantity: 1 ml Also available as FITC conjugate (MON5050F - 1 ml) Clone EFE-565 Also available as BIOTIN conjugate (MON 5050B - 1 ml) This monoclonal antibody reacts with human IgG, γ-chain. Moreover it reacts to with the heavy chains of all IgG isotypes. No cross reaction with human plasma proteins other then human IgG. No cross reaction with purified human IgA, IgE and IgM. Apparent binding constant is approximately 0.01 nM. Conjugation of the antibody with peroxidase, FITC or biotin does not impair the binding properties. Immunogen: purified human IgG. Specificity of the monoclonal was determined by ELISA on purified human immunoglobulin isotypes.

Use: F Quantity: 1 ml Also available as FITC conjugate (MON 5014F - 1 ml) Also available as HRP conjugate (MON 5014P - 1 ml)

106 www.monosan.com For research use only. Not for drug, diagnostic or other use. 107 Immunoglobulins Immunoglobulins

IgG, Mouse IgG2a kappa against light chain of mouse Ig Ig lambda-chain IgM + IgG + IgA, Chicken

Cat.no. MON 5052 (Monoclonal) Cat.no. MON 5083 (Monoclonal) Cat.no. MON 5047 (Monoclonal) Cat.no. MON 5044 (Monoclonal)

Clone LO-MG-7 Clone LO-MK-1 Clone ICO-106 Clone 408-6.1 Mouse Gamma Heavy Chain of Immunoglobulin (determined by Mouse Gamma Light Chain of Immunoglobulin (determined by ICO-106 detects human Ig λ-chain on B-cells. ICO-106 does not react The antibody reacts with anti Chicken IgM + IgG + IgA immunodot). In immunoaffinity chromatography and in ELISA test, immunodot) and exhibits no reactivity to mouse lambda light chain. with T-cells, monocytes, granulocytes or erythrocytes. Source: A BALB/c the antibody shows a poor reactivity with the IgG1 subclass. Avidity: No detectable reactivity with human IgG as tested by ELISA. mouse was immunized with human Ig and isolated splenocytes were Use: E, IB 10 1 on IgG1: 1 * 10 M- fused with P3XAg8.653 cells. Quantity: 100 µg 10 1 on IgG2a: 1 * 10 M- Use: E, C, IF, WB 10 1 on IgG2b: 1 * 10 M- Quantity: 1 ml Use: F, C on Ig : 1 * 1010 M-1 Quantity: 100 µg G3 IgY, Chicken Use: E, F IgG2b, Mouse Quantity: 1 ml IgM, Chicken Cat.no. MON 5048 (Monoclonal) Also available as FITC conjugate (MON5052F - 1 ml) Also available as HRP conjugate (MON5052P - 1 ml) Cat.no. MON 5055 (Monoclonal) Clone 7C2 Also available as BIOTIN conjugate (MON 5052B - 1 ml) Cat.no. MON 5046 (Monoclonal) The antibody can be used during various purification steps of IgY. The Clone LO-MG2b-2 yolk of eggs laid by immunized chickens has been recognized as an Mouse Gamma 2b Heavy Chain of Immunoglobulin (determined by Clone 408-5.1 excellent source of polyclonal antibodies (pAb). Specific antibodies immunodot). The antibody reacts with anti Chicken IgM. produced in chickens offer several important advantages over producing IgG1 Mouse 10 1 Avidity on IgG2b: 1 * 10 M- . No cross reactivity with other animals. antibodies in other mammals. Because a single egg contains as much Use: E, IB antibody as an average bleed from a rabbit, this simple, non-invasive Cat.no. MON 5053 (Monoclonal) Use: E, F Quantity: 100 µg approach presents an appealing alternative to conventional pAb Quantity: 1 ml production methods. Purification of chicken egg yolk immunoglobulin Clone LO-MG1-2 Also available as FITC conjugate (MON5055F - 1 ml) Y (IgY), the 150 kDa IgG homolog, does not require animal bleeding. Mouse gamma 1 heavy chain of immunoglobulin (determined by Also available as HRP conjugate (MON5055P - 1 ml) IgM In addition, the eggs from immunized chickens provide a continual, immunodot). Also available as BIOTIN conjugate (MON 5055B - 1 ml) daily source of pAb, and this convenient approach offers greater 8 1 Avidity on IgG1: 9 * 10 M- . No cross reactivity with other animals. compatibility with animal protection regulations. Due to the phylogenetic Cat.no. MON 5015 (Monoclonal) distance between birds and mammals, there is greater potential of Use: E, F IgG3, Mouse producing a higher percentage of specific antibody against mammalian Quantity: 1 ml Clone ICL-931 antigens when using chickens. Highly conserved mammalian proteins Also available as FITC conjugate (MON5053F - 1 ml) This monoclonal antibody reacts with human IgM, µ-chain. No cross sometimes fail to illicit a humoral response in animals, such as rabbits, Also available as HRP conjugate (MON5053P - 1 ml) Cat.no. MON 5056 (Monoclonal) reaction with human plasma proteins other then human IgM. No cross that are traditionally used for generating pAb. Non-specific binding Also available as BIOTIN conjugate (MON 5053B - 1 ml) reaction with purified human IgA, IgE and IgG. Apparent binding and need for cross-species immunoabsorptions is eliminated since Clone LO-MG3-7 constant is approximately 0.01 nM. Conjugation of the antibody with chicken IgY does not cross react with mammalian IgG and does not Mouse Gamma 3 Heavy Chain of Immunoglobulin (determined by peroxidase, FITC or biotin does not impair the binding properties. bind bacterial or mammalian Fc receptors. There are well defined immunodot). Immunogen: purified human IgM. Specificity of the monoclonal was structural differences of IgY-type immunoglobulins and the IgG of IgG2a, Mouse 10 1 Avidity on IgG3: 2 * 10 M- . Cross reactivity with: Horse (+), swine determined by ELISA on purified human immunoglobulin isotypes. mammals. That includes the molar mass of the heavy chains of the (++) and dog (+/-). immunoglobulins. The IgY-type immunoglobulins are much less flexible Cat.no. MON 5054 (Monoclonal) Use: E, F than IgG. Also, the structures of the Fc part of the immunoglobulin Use: E, F Quantity: 1 ml isotypes IgY and IgG are different. Clone LO-MG2a-2 Quantity: 1 ml Also available as FITC conjugate (MON 5015F - 1 ml) Mouse Gamma 2a Heavy Chain of Immunoglobulin (determined by Also available as FITC conjugate (MON5056F - 1 ml) Also available as HRP conjugate (MON 5015P - 1 ml) Use: WB, IP immunodot). Also available as HRP conjugate (MON5056P - 1 ml) Quantity: 100 µg 9 1 Avidity on IgG2a: 7 * 10 M- . No cross reactivity with other animals. Also available as BIOTIN conjugate (MON 5056B - 1 ml) Also available as BIOTIN conjugate (MON 5048B - 50 µg) IgM, Mouse Also available as HRP conjugate (MON 5048P - 50 µg) Use: F Quantity: 1 ml IgG4 Also available as HRP conjugate (MON5054P - 1 ml) Cat.no. MON 5057 (Monoclonal) Also available as BIOTIN conjugate (MON 5054B - 1 ml) Cat.no. MON 5034 (Monoclonal) Clone LO-MM-9 Mouse mu heavy chain of immunoglobulin (determined by immunodot). Clone BL-G4/1 Avidity on IgM: 7 * 108 M-1. No cross reactivity with other animals.

The antibody is highly specific for human IgG4. Use: E, F Use: E Quantity: 1 ml Quantity: 1 ml Also available as FITC conjugate (MON5057F - 1 ml) Also available as BIOTIN conjugate (MON 5057B - 1 ml)

108 For research use only. Not for drug, diagnostic or other use. www.monosan.com 109 Keratins Keratins

Keratin Keratin 5 and 8 Keratin 7, 54 kD Keratin 8 and 18

Cat.no. PS033 (Polyclonal) Cat.no. MON 3029 (Monoclonal) Cat.no. MON 3007 (Monoclonal) Cat.no. MON 3009 (Monoclonal)

The polyclonal antibodies react with keratin of most mammals and birds. Clone C-50 Clone RCK105 Clone NCL5D-3 A positive reaction is obtained with all stratified squamous epithelia This monoclonal antibody reacts with human keratin 5 and human The monoclonal antibody RCK 105 is specifically reactive with keratin This monoclonal antibody reacts in immunoblots with the 52.2 kD and and in epidermal appendages such as hair follicles and sebaceous keratin 8. The antigen isolated for immunization came from HeLa cells. 7. In immunoblots it reacts specifically with the 54 kD keratin protein 45 kD cytokeratin proteins (no. 8 and 18 in the Moll-catalogue). glands. Also the epithelia of the urinary tract, stomach, small and large Cross reactivity is observed with Keratin from most mammals but not (keratin 7). It shows crossreactivity with keratin 7 from human, hamster, Antigen is isolated from human breast carcinoma cell line MCF7. intestine, uterine endometrium, bile ducts, prostate, kidney collecting with chicken or Xenopus. mouse, rat, canine and swine. Antigen location: cytoplasm. Cross reactivity: no significant reactivity tubules, thymic reticulum cells, mesothelium and to a lesser extent with mouse or rat tissues. hepatocytes, adenoncarcinomas, transitional cell carcinomas and Use: F, P, WB Use: F, C, WB anaplastic carcinomas can be identified by this antiserum. Renal cell Quantity: 100 µg Quantity: 1 ml Use: F, P adenocarcinomas and small cell anaplastic lung tumours may remain Quantity: 1 ml negative, when examined in paraffin sections. Hepatocytes and pancreatic acini show a positive staining. Immunogen: keratin extracted Keratin 5 and 8, 58/52 kD Keratin 7 (54kDa) and 17 (46kDa) from human skin. Antigen location, cytoplasma. Cross reactive with Keratin 8 keratin from most mammals and birds. In immunohistochemistry nearly all cultured epithelial cell are positive, all non-epithelial cells are negative. Cat.no. MON 3008 (Monoclonal) Cat.no. MON 3019 (Monoclonal) Cat.no. MON 3032 (Monoclonal) Use: F, P Clone RCK102 Clone C-46 Quantity: 0.25 ml This monoclonal antibody reacts in immunoblots with the 58 kD and The antibody reacts with the human keratin 7 (54kDa) and 17 (46kDa). Clone C-51 52.2 kD cytokeratin proteins (no. 5 and 8 in the Moll-catalogue). Cross The antigen isolated for immunization came from HeLa cells. Cross This monoclonal antibody reacts with the 52.5 kDa human keratin 8. reactivity is observed with Keratin from most mammals. Immunogen: reactive with: weak positive pig, sheep and dog tissues and positive The antigen isolated for immunization came from HeLa cells. Cross Keratin 2e lung cancer cell preparation. Antigen location: cytoplasm. Cross reactivity with bovine tissue. No cross reactivity with rabbit, mouse and rat tissue. reactive with sheep, pig and bovine epithelial tissues.. No cross reactivity with keratin from most mammals. with other mammalian species (dog, rabbit, rat, mouse) as well as Use: F, P, IF, WB chicken and Xenospus tissues are negative. Cat.no. MON 3072 (Monoclonal) NEW Use: F, P Quantity: 100 µg Quantity: 1 ml Use: F, P, WB Clone Ks 2.342.7.1 Quantity: 100 µg This antibody represents an excellent marker to study terminal epidermal Keratin 8 differentiation. It is reactive with epidermal cells in uppermost suprabasal Keratin 6 layers including scalp, foot and sole. It recognizes individual cells within Keratin 9 epidermis of tongue and mamille (co-localization with cytokeratin 10). Cat.no. MON 3016 (Monoclonal) It is also reactive on hyperkeratosis of diverse viral and genetic origin. Cat.no MON 3070 (Monoclonal) NEW The antibody does not react with palate cytokeratin 2p. Clone M20 Cat. no. MON 3075 (Monoclonal) NEW Antigen recognized in species: Human, bovine (calf hoof epidermis, Clone Ks6.KA12 The antibody does stain almost all epithelial cells. In immunoblotting snooze epithelium; negative with tongue epithelium), mouse/rat The antibody represents an excellent marker for non-keratinized experiments the antibody recognizes only keratin no. 8 (52,2 kD) and Clones Ks 9.70 / Ks 9.216 (heterogeneously on foot sole epidermis only; negative with epidermis squamous epithelia and proliferating cells of epidermis (e.g. within does not cross react with other cytokeratins. Immunogen: Cytokeratin The antibody reagent represents an excellent marker to study palmo- of other body sites) psoriatic lesions). purified from the human breast carcinoma cell line MC-7. plantar epidermal distribution and differentiation. Specifically reactive in the middle/upper suprabasal layers (stratum spinosum/granulosum) Use: F, P, WB Use: F, P, WB Use: F, P, WB of the epidermis of palm and sole. CK9 can be detected in primary Quantity: 1 ml Quantity: 1 ml Quantity: 1 ml cultures of palmoplantar keratinocytes when they shift to differentiation- promoting conditions and grow stratified (upper cells). CK9 has not been found in epithelial cells of other stratified epithelia such as oesophagus Keratin 4 Keratin 7 Keratin 8 or complex epithelia (e.g. urothelium) or in ductal or simple epithelia. The antibody cocktail is an excellent new tool to characterize primary cultures of keratinocytes/skin transplants for application in burn Cat.no. MON 3015 (Monoclonal) Cat.no. MON 3014 (Monoclonal) Cat.no. MON 3035 (Monoclonal) treatment.

Clone 6B10 Clone OVTL12/30 Clone C-43 Use: F, P, WB The antibody does stain non-cornifying squamous epithelium, cells in OVTL12/30 reacts specifically with Keratin 7. It stains a 54 kD protein This monoclonal antibody reacts with the 52.5 kDa human keratin 8. Quantity: 1 ml certain ciliated pseudo-stratified epithelia and ductal epithelia of band of cytoskeleton proteins from cell lines in one and two dimensional The antigen isolated for immunization came from cytoskeleton various exocrine glands. In immunoblotting experiments the antibody immunoblots. preparation of HeLa cells. Cross-reactive with a wide variety of mammals recognizes only keratin no. 4 (59kD) and does not show any cross such as sheep, pig, bovine and rabbit but no cross reactivity with rat, reactivity with other cytokeratins. Immunogen: Cytokeratin preparation Use: P mouse chicken and Xenopus tissues. purified form human esophagus. Quantity: 1 ml Use: F, P, WB Use: F, P Quantity: 100 µg Quantity: 1 ml (MON 3015-1) 5 ml (MON 3015-5)

110 For research use only. Not for drug, diagnostic or other use. www.monosan.com 111 Keratins Keratins

Keratin 10 Keratin 13 Keratin 17 Keratin 18

Cat.no. MON 3025 (Monoclonal) Cat.no. MON 3018 (Monoclonal) Cat.no. MON 3073 (Monoclonal) NEW Cat.no. MON 3026 (Monoclonal)

Clone DE-K10 Clone 2D7 Clone Ks 17.E3 Clone RGE53 This monoclonal antibody reacts in immunoblots with the 56.6 kD This antibody does stain non-cornifying squamous epithelium. In This antibody represents an excellent marker to distinguish myoepithelial Keratin RGE 53 specifically recognizes glandular epithelial cells from cytokeratin protein. The antibody DE-K10 specifically recognizes immunoblotting experiments the antibody recognizes only keratin no. cells from luminal epithelium of various glands (mammary, sweat, gastro-intestinal tract, respiratory tract and urogenetical tract. As well cytokeratin 10. The antibody can therefore be used for the visualisation 13 (54kD) and does not show any cross reactivity with other keratins. salivary, bronchial, tracheal, laryngeal, esophageal) and benign from as endocrine and exocrine tissues and mesothelial cells. It is unreactive of keratinizing cells in squamous cell carcinomas from epidermis, lung, The used immunogen was a keratin preparation purified from human malignant forms of e.g. mammary gland tumors. Most malignant forms with stratified squamous epithelia (esophagus, epidermis, ectocervix). bladder, cervix, oesophagus, etc. Origin of antigen: cytoskeleton esophagus. of mammary tumors are negative for cytokeratin 17. In transitional epithelium of the bladder RGE53 only reacts with the preparation from human epidermis. Antigen location: cytoplasm. superficial (umbrella) cells. RGE53 can distinguish adenocarcinomas Use: F, P Use: F, P, WB and mesotheliomas from squamous cell carcinoma and non-epithelial Use: F, P Quantity: 1 ml Quantity: 1 ml tumours. The antibody is directed against 45 kD cytokeratin protein Quantity: 1 ml (cytokeratin 18). Cross reactivity with; Keratin from most mammals, birds.

Keratin 14 Keratin 18 Use: F, WB, C Keratin 10 Quantity: 1 ml Cat.no. MON 3043 (Monoclonal) Cat.no. MON 3036 (Monoclonal) Cat.no. MON 3010 (Monoclonal) Keratin 18 Clone RCK107 Clone DA-7 Clone RKSE60 Immunoblotting: antibody reacts with a protein of aproximately 50 kD The antibody reacts with a 45 kDa cytokeratin protein (cytokeratin 18). This monoclonal antibody reacts in immunoblots with the 56.6 kD corresponding to Keratin 14. The monoclonal antibody RCK107 is Keratin DA-7 specifically recognizes glandular epithelial cells from Cat.no. MON 3023 (Monoclonal) cytokeratin protein (no. 10 in the Moll-catalogue). It reacts specifically reactive with Keratin 14. In normal tissue cytokeratin 14 is mainly gastro-intestinal tract, respiratory tact and urogenetical tract, as well with keratinizing squamous epithelia. It can be used for the recognition expressed in Basal cells of the larynx, esophagus, trachea, bladder, as endocrine and exocrine tissue and mesothelial cells. It is not reactive Clone DC-10 of keratinizing cells in squamous cell carcinomas from epidermis, lung, prostate, cervix, vagina, breast acine, skin and sweat glands. In several with stratified squamous epithelia (Esophagus, epidermis and ectocervix). This antibody reacts with the 45 kDa human keratin 18. The antigen bladder, cervix, oeso-phagus etc. Furthermore, this antibody can be studies the correlation with the keratin expression of basal cells and In transitional epithelium of the bladder it reacts only with the isolated for immunization came from breast carcinoma cell line PMC-42. applied in research of skin diseases since it reacts only with the the distribution of the basement proteins in different types of carcinomas superficial (umbrella) cells. DA-7 can distinguish adenocarcinomas and No cross reactivity with cow, pig, sheep, dog rabbit, rat or mouse. keratinizing (suprabasal) cells of the epidermis and not with the basal is described. mesotheliomas from squamous cell carcinoma and non-epithelial cells. Cross reactivity is observed with keratin from rat and bovine tumours. Not cross reactive with a wide variety of mammalian species. Use: F, P, E, WB tissues. Use: F, C, WB Antigen origin: human breast carcinoma cell line PMC-42. Antigen Quantity: 100 µg Quantity: 1 ml location: Cytoplasm. Use: F, P Quantity: 1 ml Use: F, P, WB, E Keratin 18 Keratin 14 Quantity: 100 µg Keratin 13 Cat.no. MON 3022 (Monoclonal) Cat. no. MON 3074 (Monoclonal) NEW Keratin 18 Clone Ks 18.04 Cat.no. MON 3017 (Monoclonal) Clone DE-SPK14 This antibody reacts with the 45kDa polypeptide of all simple type DE-SPK14 reacts specifically in immunoblotting with human keratin 14 Cat.no. MON 3034 (Monoclonal) epithelia and basal cells of many squamous non-epidermal epithelia. Clone 1C7 (50 kD). In tissue sections this mAb provides a positive reaction on Ks 18.04 represents an excellent marker to discriminate simple epithelia The antibody does not stain non-cornifying squamous epithelium. In basal cells of non-keratinizing stratified epithelia, basal cells and Clone C-04 from those of different origin. Tumours specifically detected: All adeno immunoblotting experiments the antibody recognizes only keratin no. suprabasal cells of the epidermis and gingival, myoepithelial cells and This monoclonal antibody reacts with the 45 kDa human keratin 18. CA; mammary CA; Urinary bladder CA; undiff. CA; cervix CA; hepato- 13 (54kD) and does not show any cross reactivity with other squamous cell carcinomas. The antigen isolated for immunization came from cytoskeleton cellular CA. cytokeratins. Immunogen: keratin preparation purified from human preparation of human A 431 carcinoma line. Cross-reactive with a wide esophagus. Use: F, P variety of mammals. Use: F, P, WB Quantity: 1 ml Quantity: 50 µg Use: F, P Use: F, P, WB Quantity: 1 ml Quantity: 100 µg

112 www.monosan.com For research use only. Not for drug, diagnostic or other use. 113 Keratins Keratins

Keratin 18 Pan Keratin Epithelial Keratin Type I Epithelial Keratin Type I & II

Cat.no. MON 3027 (Monoclonal) Cat.no. MON 3003 (Monoclonal) Cat.no. MON 3037 (Monoclonal) Cat.no. MON 3076 (Monoclonal) NEW

Clone RCK 106 Clone 80 Clone AE-1 Clones AE-1 / AE-3 The antibody reacts with a 45 kDa cytokeratin protein (cytokeratin 18). In immunoblots most human keratins are recognized. This antibody has shown to recognize most of the acidic (type I) keratins. AE1 / AE3 represents an excellent marker for distinguishing carcinomas Keratin RCK 106, directed against cytokeratin 18 specifically recognizes Each epithelium contains at least one acidic keratin. In this way AE-1 from non-epithelial tumors; reacts with all epithelium-derived tumors all adenoepithelia. The antibody is not reactive with non-stratified Use: F, P is broadly reactive with almost all epithelial and there neoplasms. In and their neoplasms. Decorates the majority of type I and type II squamous epithelia. Antigen origin: human cytokeratin preparation. Quantity: 1 ml (MON 3003-1) addition, this antibody exhibits the interesting property of producing cytokeratins. Polypeptides reacting; Mr 40000, Mr 48000 and Mr 50000 Antigen location: cytoplasm. 5 ml (MON 3003-5) heterogeneous staining within a given epithelium; the detailed polypeptides of human epithelial proteins (cytokeratins nos. 19, 16, 14) Cross reactivity with rat is negative, all other unknown. staining pattern varies depending on the differentiated and/or growth and most other acidic (type I) cytokeratins (AE1); most basic (type II) state of the individual cells. This antibody together with AE-1 has been cytokeratins (Mr 52500 – 68000) of human epithelia (AE3) Use: F Keratins 4,5,6,8,10,13 and 18 used for studying the immunolocalization of epidermal keratins, tissue Quantity: 1 ml distribution and epithelia development. AE-1 and AE-3 have also been Use: F, P, WB used extensively, individually or mixed, for distinguishing carcinoma Quantity: 1 ml Cat.no. MON 3039 (Monoclonal) from non-carcinoma. The antibody is cross reactive with keratins of Keratin 18 rabbit, mouse, rat, bovine and chicken. Clone C-11 This antibody is a broad spectrum anti Keratin antibody which reacts Use: F, P, WB Cat.no. MON 3006 (Monoclonal) specifically with a variety of normal, reactive and neoplastic epithelia. Quantity: 250 µg The antibody reacts with simple epithelium and both basal and Clone M9 suprabasal layers of cornifying and non-cornifying squamous epithelia. In immunoblotting experiments the antibody recognizes only keratin Immunogen: cytoskeleton preparation of human A431 carcinoma line. Epithelial Keratin Type II no. 18 (45 kD) and lacks cross reactivity with other cytokeratins. Use: F, P, WB Use: F, P Quantity: 100 µg Cat.no. MON 3038 (Monoclonal) Quantity: 1 ml (MON 3006-1) 5 ml (MON 3006-5) Clone AE-3 Basal Keratinocytes This antibody has shown to recognize most of the basic (type II) keratins. Each epithelium contains at least one basic keratin. In this way AE-3 is Keratin 19 broadly reactive with almost all epithelial and there neoplasms. This Cat.no. MON 3013 (Monoclonal) antibody together with AE-1 has been used for studying the immuno- localization of epidermal keratins, tissue distribution and epithelia Cat.no. MON 3033 (Monoclonal) Clone PNK 1 development. AE-1 and AE-3 have also been used extensively, The antibody reacts specifically with the basal cell layer of the normal individually or mixed, for distinguishing carcinoma from non-carcinoma. Clone BA-17 human epidermis. The antigen recognized has not yet been characterized. The antibody is cross reactive with keratins of rabbit, mouse, rat, This monoclonal antibody reacts with the 40 kDa human keratin 19. The antibody does not react with normal human kidney, adrenal gland, bovine and chicken. Within immunoblots AE-1 detects distinct MW’s of The antigen isolated for immunization came from mammary organoids. heart, liver, spleen, thyroid, pancreas, brain, stomach, colon, bladder, 52 kD, 54 kD, 56 kD, 58 kD, 59 kD, 64 kD, and 65-67 kD with all Ip’s parotid gland and prostate. lower then 6.0. These MW’s are believed to correspond with respectively Use: F, P, IP, WB keratin 8, 7, 6, 5, 4, 3 and 1 & 2. Quantity: 1 ml Use: F Quantity: 1 ml Use: F, P, WB Quantity: 250 µg Keratin 19 Basal Cell Cytokeratin Cat.no. MON 3020 (Monoclonal) Cat.no. MON 3000 (Monoclonal) Clone RCK 108 The monoclonal antibody specifically reacts with the cytokeratin 19, a Clone RCK103 40 kDa polypeptide, which is found in most simple and non-keratinized A broadly cross reacting antibody staining basal cells in combined and stratified epithelia. This antibody reacts on formalin fixed, paraffin stratified epithelial tissues. Cross reactivity is observed with human, embedded material and can be used in diagnostic immunopathology. quail, chicken, rat, rabbit, hamster, canine and swine. The reaction pattern of cytokeratin 19 is documented in the literature. Antigen origin: human bladder cell line T24. Antigen location: Cytoplasm. Use: F, WB, C Quantity: 1 ml Use: F, P Quantity: 1 ml

114 For research use only. Not for drug, diagnostic or other use. www.monosan.com 115 Lymphoid Markers Lymphoid Markers

B-cells Granzyme B HLA-Class I HLA-D, MHC CLASS II, Broad Reacting

Cat.no. MON 7026 (Monoclonal) Cat.no. MON 7029 (Monoclonal) Cat.no. MON 2078 (Monoclonal) Cat.no. MON 1087 (Monoclonal)

Clone MB2 Clone GrB-7 Clone MEM-147 Clone BL-IA/6 This antibody reacts with all B-cells. Further positive reactions with The monoclonal antibody reacts with the 33 kD human serine protease MEM-147 Mab reacts with all human classical MHC Class I molecules The antibody precipitates both the 32-34 kD and a 27 kD glycoproteins epithelia and endothelial cells. In immunoblots it reacts with a 28 kD granzyme B. It does not react with human granzyme A. It is used as a in native cellsurface forms. Positive in immunoblotting with many (not specific for the MHC class II. It recognizes a non-polymorphic protein. Antigen origin: human lymphocytes. Antigen location: cytoplasm. marker for NK-cells and activated cytotoxic T-cells (CTL). This protease all) MHC Class I allelic forms. Epitope specificity is very similar to classical determinant on the β-chain (27 kD) of the HLA-D-gene products. The is localized in cytoplasmic granules and gives a granular staining pattern. antibody W6/32. antibody stains the majority of mature peripheral B-lymphocytes and Use: F, P Granzyme B is involved in target cell apoptosis during lymphocyte activated T-lymphocytes. Quantity: 1 ml mediated cyto-toxicity. Exocytosis of granzyme containing granules in the Use: C, IP, WB cytoplasm of the target cell will lead to induction of DNA fragmentation Quantity: 100 µg Use: C, F, P and apoptosis of the target cell. Quantity: 1 ml B-cells, Rat Use: P, WB HLA-Class I Quantity: 1 ml (MON 7029-1) HLA-DR Cat.no. MON 2024 (Monoclonal) 5 ml (MON 7029-5) Cat.no. MON 2079 (Monoclonal) Clone 68-IB3 Cat.no. MON 1081 (Monoclonal) The monoclonal antibody recognizes rat B-cells and is reactive with all HLA-B Clone MEM-123 common inbred strains. Mab MEM-123 was previously characterized as antibody recognizing Clone LN-3 HLA-A, -B and –C molecules. Recent studies revealed that MAb MEM-123 The mAb LN-3 reacts with human leukocyte antigen-DR (HLA-DR). The Use: F, C Cat.no. MON 1007 (Monoclonal) also reacts with native HLA-G molecule expressed by cells transfected mAb has lymph node germinal center and mantle zone B-cell reactivity. Quantity: 100 µg with full-length HLA-G cDNA. Cytofluorometric analysis showed that It reacts with interdigitating histiocytes in T-cell zones and with sinus Clone JOAN-1 MAb MEM-123 does not block immunofluorescence staining of HLA-G histiocytes and endothelial cells. It has also tumour specificity and JOAN-1 recognizes an intralocus determinant present on a limited transfectants by MEM-G/9 FITC conjugate. Mab MEM-123 reacts with reactivity with normal non-lymphoid tissue. Dendritic Cells number of HLA-B locus-encoded gene products. Source: NS1 mouse all human classical MHC Class I molecules in native cellsurface forms myeloma cells were fused with splenocytes from a BALB/c mouse as well as with human HLA-G cDNA transfected cells. Mab MEM-123 Use: P immunized intraperitoneally with normal human peripheral blood completely blocks binding of standard pan MHC Mab W6/32 and does Quantity: 1 ml Cat. no. MON 9501 (Monoclonal) lymphocytes. not block binding of Mab MEM-G/9 to surface-expressed HLA-G. Mab MEM-123 cross reacts with bovine MHC Class I molecules. Clone RFD1 Use: F, WB HLA-DR+DP, FITC conjugated Recognizes an epitope with within the class II MHC complex. In normal Quantity: 100 µg Use: C tissue it identifies dendritic cells and a proportion of B-lymphocytes. Quantity: 100 µg Evidence in pathological tissue and functional studies suggests it binds Cat.no. MON 4038F NEW to an epitope expressed by antigen presenting cells. Within the HLA-B27 macrophage/dendritic cell populations the epitope seen by RFD1 is co HLA-D Clone HL-38 expressed with RFD7 by a subset of cells that exhibit suppressive activity The antibody recognizes common epitope on human HLA-DR and HLA-DP. on T-cell stimulation. Cat.no. MON 8039 (Monoclonal) Cat.no. MON 1013 (Monoclonal) Use: C Use: F Clone EP-4 Quantity: 100 tests Quantity: 200 µg EP-4 recognizes the HLA-B27 cell surface antigen on human cells. Since Clone 7.5.10.1 EP-4 recognizes the HLA-B27 antigen, it may be used to HLA type The antibody reacts with a monomorphic determinant on MHC class II, human lymphoctyes. Approximately 60% of patients with ankylosing HLA-D region molecules. It detects the molecules of the HLA-DR, DP HLA-DR+DP Dendritic Cells, Rat spondylitis are HLA-B27 positive. This reagent can be used to help and DQ loci. It inhibits the mixed lymphocyte culture. It also reacts with identify this HLA haplotype in human lymphocytes. Monoclonal antibody MHC class II molecules of various animals and is a useful tool for studies EP-4 recognizes the HLA-B27 antigen expressed on individuals with with dogs. Cat.no. MON 1002 (Monoclonal) Cat. no. MON 2025 (Monoclonal) this HLA haplotype. Use: MC, F, IF Clone MEM-136 Clone 1F119 Use: P Quantity: 1 ml (MON 1013-1) This antibody recognizes a common epitope on the β-chain of human The monoclonal antibody recognizes rat dendritic cells from thoracic Quantity: 100 µg 5 ml (MON 1013-5) HLA-DP. Reacts with αβ-dimmer as well as with dissociated β-subunit. duct lymph, spleen, thymus, and lymphnode. 1F119 is derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Use: C, IP, WB Balb/c mice immunized with splenic dendritic cells and thoracic duct Quantity: 100 µg dendritic cells.

Use: F, C Quantity: 1 ml

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HLA-DR, MHC CLASS II HLA-DQ HLA-E HLA-G

Cat.no. MON 1082 (Monoclonal) Cat.no. MON 1058 (Monoclonal) Cat.no. MON 2077 (Monoclonal) Cat.no. MON 2076 (Monoclonal)

Clone HL-39 Clone BL-A/G3 Clone MEM-E/02 Clone MEM-G/1 The antibody bind to a common monomorphic determinant of HLA-DR The antibody precipitates both the 32-34 kD and a 27 kD glycoproteins MEM-E/02 strongly reacts with HLA-E denaturated heavy chain (43 kDa) This antibody MEM-G/1 reacts with denaturated HLA-G heavy chain. which is dependent on the association of the heavy and the light chain specific for the MHC class II antigen HLA-DQ. It recognizes a non- under conditions of Western Blotting but does not recognize native of HLA-DR molecules. The antibody recognizes the same determinant polymorphic determinant on the β-chain (27 kD) of the HLA-DQ-Locus. HLA-E by flow cytometry. Western Blotting analysis of panel cell lines Use: WB, P as mab L243. The antibody stains the majority of mature peripheral B-lymphocytes expressing distinct sets of HLA-A, B and C alleles demonstrated that Quantity: 100 µg and activated T-lymphocytes. Mab MEM-E/02 does not cross react with classical MHC Class I Use: C, IP, P molecules. Moreover, the antibody does not cross react with denaturated Quantity: 100 µg Use: F, C HLA-G molecule. HLA-G Also available as R-PE conjugate (MON1082R - 100 tests) Quantity: 1 ml Use: WB, P Quantity: 100 µg Cat. no. MON 2087 (Monoclonal) NEW HLA-DRw52 REL. DETERM. HLA-DQw1 Clone MEM-G/11 HLA-E The antibody recognizes HLA-G1 antigen. Cat.no. MON 1015 (Monoclonal) Cat.no. MON 1014 (Monoclonal) Immunogen: Recombinant human HLA-G refolded with beta2-micro- globulin and peptide. Clone -7.3.19.1 Clone IIB3 Cat. no. MON 2080 (Monoclonal) NEW The antibody reacts with a polymorphic determinant on HLA-DR The antibody reacts with a polymorphic determinant on DQ-like Use: IP, C molecules which is associated with DR3, DR5, and DRw6 (DRw52-like). molecules which is associated with DR1, DR2 and DRw6. In addition it Clone MEM-E/06 Quantity: 100 µg It does not react with DRw8. The antibody reacts with part of the HLA-D reacts with a part of the DR4, DR7, DRw8 and DRw9 positive cells. The The antibody recognized native surface-expressed HLA-E, but not Also available as FITC conjugate (MON2087F - 100 tests) molecules (29+34 kD). The determinant is strongly expressed on DR3 determinant is strongly expressed on DR2, DR4 and DRw6 positive cells denaturated heavy chain of HLA-E. The published results showed that positive cells and more weakly on DR5 and DRw6 positive cells. Cross but only weakly on DR1 and DR7 positive cells. The antibody reacts the antibody cross-reacts with some classical MHC Class I molecules reacts with Chimpanzee, Macaca-apes and gorilla. mainly with B-lymphocytes and not or with a minority of the monocytes. (HLA-A3, -A11, -B7). However, the recent Workshop I Session on the Kappa Light Chain 3rd International Conference on HLA-G (Paris, July 2003) confirmed Use: IF, MC Use: IF, MC that the antibody exhibits much broader cross-reactivity classical MHC Quantity: 1 ml (MON 1015-1) Quantity: 1 ml (MON 1014-1) Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, Cat.no. MON 5001A (Monoclonal) 5 ml (MON 1015-5) 5 ml (MON 1014-5) -B44, -B54, -C3, -C4, -C5, -C7. Clone 2B7 Use: C, IP The antibody reacts with free kappa chains, but not with kappa chains HLA-DR+DP, MHC CLASS II HLA-DQ1+DQ3, MHC CLASS II Quantity: 100 µg attached to heavy chains. No reaction is observed with Ig kappa on membranes of lymphocytes. No cross reactivity with lambda chains. In Ouchterlony immunodiffusion no reaction with Bence Jones kappa Cat.no. MON 1083 (Monoclonal) Cat.no. MON 1084 (Monoclonal) HLA-G is observed. Clone HL-40 Clone HL-37 Use: F, P The antibody binds to a common monomorphic determinant of the light The antibody binds to a common antigenic determinant of the beta chain Cat.no. MON 2075 (Monoclonal) Quantity: 1 ml (MON 5001A-1) chain of HLA-DR and HLA-DP antigens. The antibody reacts also with of HLA-DQ1 and HLA-DQ3. The specificity of this mab is similar to mab 5 ml (MON 5001A-5) Porcine MHC Class II antigens, SLA-D gene products. BT3/4 and Leu-10 (1,2). Clone MEM-G/9 This antibody reacts with the native form of human HLA-G on the cell Kappa Light Chain Use: C, IP Use: F surface as well as with soluble HLA-G molecules in its _2-microglobulin Quantity: 100 µg Quantity: 100 µg associated form. Specific reactions was demonstrated with cells transfected with either full-length HLA-G and HLA-G1 complementary Cat.no. MON 8050 (Monoclonal) DNA. Clone L1C1 Use: C, E, F L1C1 reacts with the kappa light chain of human immunoglobulins. Quantity: 100 µg Source: A BALB/c mouse was immunized with B-lymphoma cells. Also available as FITC conjugate (MON 2075F - 100 tests) Spleen cells were fused with SP2/0 myeloma cells. Also available as R-PE conjugate (MON2075R - 100 tests) Use: F, P, E Quantity: 100 µg

118 www.monosan.com For research use only. Not for drug, diagnostic or other use. 119 Lymphoid Markers Lymphoid Markers

Lambda Light Chain Macrophage MRP14 Myeloid specific antigen

Cat.no. MON 5002 (Monoclonal) Cat.no. MON 7044 (Monoclonal) Cat.no. MON 9042 (Monoclonal) Cat.no. MON 2037 (Monoclonal)

Clone 48 Clone LN-5 Clone 47-8D3 Clone BM-2 In ELISA the antibody will react with free lambda chains and also with LN-5 reacts with human macrophages. The mAb has lymph node 47-8D3 reacts with macrophages and detects the well-known Until recently, immunological markers for myeloid cells have been lambda chains attached to heavy chains. In Ouchterlony immuno- germinal centre and mantle zone B-cell reactivity. It reacts with leukocyte L1, cystic fibrosis antigen. Using Western blot analysis 47-8D3 lacking, especially those which identify different levels of cellular diffusion, no reaction with Bence Jones lambda or Ig lambda. No cross interdigitating reticulum cells, with tingible body and sinus histiocytes. was found to detect a single protein band of 14 kD in lysates of human differentiation. The BM series provides a new panel of monoclonal reactivity with kappa chains. It has also tumour specificity and reactivity with normal non-lymphoid monocytes and granulocytes, which was demonstrated to be the antibodies which stain early precursor and mature forms of human tissue. Source: BALB/c mice were hyper immunized with deparaffinized calcium-binding protein MRP14. Source: A BALB/c mouse was immunized myeloid cells. This panel of monoclonal antibodies reacts with antigenic Use: F, P cell extracts of peripheral blood mononuclear cells. From nuclei from with human peripheral blood monocyte components. Splenocytes were determi-nants present in normal myeloid cells and leukaemias of Quantity: 1 ml (MON 5002-1) pokeweed mitogen-stimulated PBL. Spleen cells were fused with mouse fused with mouse myeloma cell line NS-1. similar derivation. BM-2 recognizes a cytoplasmic antigen expressed in 5 ml (MON 5002-5) myeloma NS-1 cells. mature human granulocytes (polys) residing in lymphoid and non- Use: F, P, E, WB lymphoid tissues. It does not react with any other cell type in human Use: F, P Quantity: 100 µg tissues. Source: Nuclei from Pokeweed mitogen stimulated human Leukocyte common antigen- like, Rat Quantity: 100 µg peripheral blood lymphocytes were used as immunogen. MHC class II, Rat Use: F Cat.no. MON 2026 (Monoclonal) Macrophage, Rat Quantity: 100 µg Clone 50-4B1 Cat.no. MON 2028 (Monoclonal) The monoclonal antibody recognizes rat leucocyte common antigen Cat.no. MON 2027 (Monoclonal) Myeloid Specific Antigen (MW 70 kDa) and is reactive with all leucocytes. Clone 53-5D2 Clone F-6-J The monoclonal antibody recognizes rat MHC class II and is reactive Use: F, C The monoclonal antibody recognizes rat macrophages and is reactive with all common inbred strains. Cat.no. MON 2051 (Monoclonal) Quantity: 100 µg with all common inbred strains. Use: F, C Clone BM-3 Use: F, C Quantity: 1 ml BM-3 recognizes a nuclear antigen expressed in human granulocytes Macrophage Quantity: 100 µg (98%) and monocytes (80%) residing in lymphoid and non-lymphoid Myeloid cells tissues. BM-3 is an early marker of myeloid differentiation. Immuno- precipitation experiments using S-35 methionine labelled human myeloid Cat.no. MON 7047 (Monoclonal) Mature macrophages leukaemia cells show that BM-3 identifies a 13,000 Dalton protein. Cat.no. MON 2036 (Monoclonal) BM-3 is specific marker for human myeloid cells. Induction studies Clone EP-3 using HL-60 cells show that BM-3 identifies a nuclear antigen which is Monoclonal antibody EP-3 recognizes an antigen associated with the Cat. no. MON 3097 (Monoclonal) NEW Clone BM-1 expressed during the early phases of myeloid differentiation. cytoplasm of human macrophages resident in the thymus and other BM-1 is reactive in B5 fixed, paraffin embedded tissue sections to human lymphoid tissues. Monoclonal antibody EP-3 can be used to stain cortical Clone 25F9 myeloid cells and derived malignancies. The antibody reacts with a Use: F thymic macrophages and macrophages in lymphoid and non-lymphoid The monoclonal antibody 25F9 recognizes a protein of 86 kD on the 183,000 Dalton cytoplasmic antigen with DNA-binding characteristics Quantity: 100 µg tissues using routinely fixed and embedded tissues. Thymic tumours cell surface and within the cytoplasm of mature macrophages. The which is expressed in most myeloid precursor cells and myeloid may be derived from different cellular components. This monoclonal antibody is associated with fully differentiated tissue macrophages leukaemias. It’s positive on myeloid precursors in bone marrow but antibody can be used to identify cortical macrophages and tumours both in normal and in diseased tissues, independently of the presence lymph nodes are negative. Tissue granulocytes are positive as well as Myeloid Specific Antigen derived from this cell type. or absence of inflammation. The antigen is absent on freshly isolated scattered cells in peripheral cortex and interlobular septae of adult and monocytes and other blood cells. After 6 to 7 days culture human fetal hymus. Portal regions of fetal liver (18 weeks) are also positive. Use: P monocytes become positive. Some melanoma and carcinoma cell lines Cat.no. MON 2052 (Monoclonal) Quantity: 100 µg are also positive. Furthermore the monoclonal antibody 25F9 cross Use: F, P reacts with a subpopulation of macrophages of rhesus monkey, pig Quantity: 100 µg Clone BM-4 alveolar macrophages and Kupffer cells. BM-4 recognizes a nuclear antigen expressed in human granulocytes Macrophage The monoclonal antibody 25F9 is very useful for macrophage (83%) and monocytes (20%) residing in lymphoid and non-lymphoid phenotyping. tissues. 856-4 is an early marker of myeloid differentiation. BM-4 is specific marker for human myeloid cells. Induction studies using HL-60 Cat.no. MON 7023 (Monoclonal) Use: WB, C, F, P cells show that 856-4 identifies a nuclear antigen which is expressed Quantity: 100 µg during the early phases of myeloid differentiation. Clone 3A5 This antibody stains macrophages and histiocytic cells in a wide variety Use: F of normal and diseased tissue. Dendritic cells, myeloid cells and glia Quantity: 100 µg cells do not react with this monoclonal antibody. Monocytes stain weakly with 3A5, also after external activation.

Use: F, P Quantity: 1 ml

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Myeloid Specific Antigen Pan Macrophages, F4/80, Mouse T-Lymphocytes, T4

Cat.no. MON 2053 (Monoclonal) Cat. no. MON 3099 (Monoclonal) NEW Cat. no. MON 1024 (Monoclonal)

Clone BM-5 Clone BM8 Clone B-F5 BM-5 recognizes a nuclear and cytoplasmic antigen expressed in human The monoclonal antibody BM8 recognizes a 125 kD extracellular This is one of two antibodies that can be used for the determination of granulocytes, monocytes, and myeloid precursor cells. BM-5 is an early macrophage membrane molecule, highly restricted to mature T4/T8 ratio in peripheral blood specimens and immunophenotyping of marker of myeloid differentiation. BM-5 is specific marker for human macrophage subpopulations residing in tissue. This murine F4/80 malignant T-cell lymphomas. Furthermore, this antibody can be an myeloid cells. glycoprotein contains seven-transmembrane (TM7) regions, which important tool for in situ analysis of T-cell subpopulations in infectious anchor the protein in the cell membrane, and thereby shows similarity diseases and autoimmune conditions. In immunoblotting this antibody Use: F in this region to G-protein-coupled receptors. The F8/40 molecule detects the 54 kDa T4-antigen. In immunohistochemistry: 50-60% of Quantity: 100 µg shares overall structural homology to other members of the epidermal human peripheral T-lymphocytes and 80% of thymocytes stained growth factor (EGF)-TM7 family. The antigen is detected on tissue positive. Immunogen is isolated from human T-lymphocytes. fixed macrophages in all organs tested so far (spleen, lymph nodes, p80NPM/ALK thymus, liver, skin). It is also present on Langerhans cells in the skin Use: F and Kupffer cells in the liver. It is absent on granulocytes, lymphocytes Quantity: 1 ml and trombocytes. The expression of F4/80 increases upon maturation Cat.no. PS 084 (Polyclonal) of macrophage precursors in bone marrow and blood as well as in ontogeny. This antibody reacts with the chimeric protein p80NPM/ALK that is The monoclonal antibody BM8 is the only macrophage marker that is expressed specifically in Ki-1 lymphomas (anaplastic large cell able to distinguish non-destructive from destructive inflammation lymphomas), bearing t(2;5)(p23;q35) translocations. The t(2;5)(p23;q35) processes in the pancreas. Furthermore it is a unique histological translocation is observed in approximately 40% of Ki-1 lymphomas. marker of the progression from peri-insulitis to beta-cell destruction The fusion of nucleophosmin (NPM, nucleolar phosphoprotein) gene and diabetes in a mouse diabetes model. with anaplastic lymphoma kinase (ALK, protein tyrosine kinase) gene The monoclonal antibody BM8 cross reacts with human heart produces this 80 kD chimeric protein. This antibody may react with ALK macrophages. gene product as well. Immunogen: synthetic peptide SNQEVLEFVTSGGR, corresponding to the kinase domain of ALK, bound to bovine Use: WB, C, IP, F, P thyroglobulin. Quantity: 100 µg

Use: P, IC Quantity: 1 ml Pan T-Cells, Rat

Cat.no. MON 2029 (Monoclonal)

Clone 15-16A1 The monoclonal antibody recognizes rat pan T-cells (MW 95 KDa) and is reactive with all common inbred strains.

Use: F, C Quantity: 100 µg

Proteinase Inhibitor 9

Cat.no. MON 2065 (Monoclonal) NEW

Clone PI9-17 The human intracellular serpin proteinase inhibitor 9 (PI9) is the only human protein able to inhibit the activity of the serine protease granzyme B. Granzyme B is expressed by cytotoxic lymphocytes and induces rapid target cell apoptosis. PI9-17 Mab was selected after immunization with full length recombinant PI9 produced in Escherichia coli. No cross reactivity with other, homologous serpins (PI6, PI8 and PAI-2) was observed.

Use: IC, WB, C Quantity: 250 µg

122 For research use only. Not for drug, diagnostic or other use. www.monosan.com 123 Microbiology / Virology Microbiology / Virology

Campylobacter jejuni Escherichia coli 0-111 Herpes Simplex Virus Types 1 and 2 HIV protease

Cat.no. MON 2002 (Monoclonal) Cat.no. MON 2011 (Monoclonal) Cat.no. MON 7080 (Monoclonal) Cat.no. MON 4051 (Monoclonal)

Clone 57-24 Clone 3-12 Clone T111 Clone 1696 The antibody reacts with a cell wall component of C. jejuni and C. coli. The antibody reacts with the O-antigen of E. coli O-111. The antibody This antibody recognizes the gB antigen of both HSV type 1 and type 2. This antibody recognizes the free N-terminus of mature HIV protease The Hippurate reaction was considered as a marker for C. jejuni. The is also reactive with E. coli O-111 using ELISA. It does not react with (HIV-1 and HIV-2). Does not react with precursor. antibody does not react with C. fetus or C. laridus. other E. coli bacteria and other members of the Enterobacteriaceae. Use: IC, E Quantity: 100 µg Use: E, WB, FS Use: E Use: E Quantity: 100 µg Quantity: 1 ml (MON 2002-1) Quantity: 1 ml (MON 2011-1) 5 ml (MON 2002-5) 5 ml (MON 2011-5) Herpes Simplex Virus Type 1 HIV p24 protein Chlamydia trachomatis Escherichia coli K 1 Cat.no. MON 7081 (Monoclonal) Cat.no. MON 4052 (Monoclonal) Clone T96 Cat.no. MON 8004 (Monoclonal) Cat.no. MON 2004 (Monoclonal) This antibody recognizes the gC antigen of HSV type 1. Clone ND1 This antibody recognizes the HIV-1 p24 protein. Clone 3 Clone 58-5 Use: IC, E, WB The antibody is directed to an immune dominant epitope (location The antibody reacts with a cell wall component of E. coli K1. A subclass Quantity: 100 µg Use: E, WB unknown) of the Chlamydia LPS. This LPS is a genus specific antigen. of this capsule type is determined since the antibody reacts with approx. Quantity: 100 µg As ascer-tained by the Dutch State Institute of National Health using a 50% of known E. coli K1. No reactivity is observed with an OAC- form DOT-EIA the antibody reacts strongly with following prototype strains: of E. coli K1, also not with E. coli. 022K24; 078K80; 023K18; 077K96; Herpes Simplex Virus Type 2 C. trachomatis: A B Ba C D E F G H I J K L L1 L2 L3 and mouse 0127K63; K12; 06K-; 018K5; 06K53; 04K12; 07K- and 07K1 boiled for HNP 1-3 (Human Neutrophil Defensins 1-3) pneumonitis, psittaci: an ornithosis strain and a cat conjunctivitis strain, 1 hour. C. pneumoniae: TW183 Cat.no. MON 7082 (Monoclonal) Use: E, A Cat. nr. MON 2062 (Monoclonal) Use: P Quantity: 1 ml (MON 2004-1) Clone T303 Quantity: 1 ml (MON 8004-1) 5 ml (MON 2004-5) This antibody recognizes the gG antigen of HSV type 2. Clone D21 5 ml (MON 8004-5) Human neutrophil defensins (α-defensins) belong to the family of Use: IC, E, WB cationic trisulfide-containing microbicidal peptides. Besides microbicidal, Helicobacter pylori Quantity: 100 µg the peptides exert chemotactic, immunomodulating and cytotoxic Cytomegalomavirus, LA activity and participate in host defense and inflammation. Azurophilic granules of neutrophils contain Human Neutrophil Peptide (HNP) 1-4 Cat.no. MON 2007 (Monoclonal) HIV-1 gp120 which are highly homologous. The three principal human defensins, Cat.no. MON 8009 (Monoclonal) HNP 1-3, are unique to neutrophils and account for about 99 of the Clone 51-13 total defensin content of these cells. Defensins HNP 1-3 are absent in Clone BM 219 The antibody reacts specifically with Helicobacter bacteria. It does not Cat.no. MON 4050 (Monoclonal) other leukocyte subsets, thus representing neutrophil specific cell marker. The specificity of the antibody was ascertained by immunofluorescence react with other members of the Enterobacteriaceae. Measured amount of defensins is 3 -5 mg per million human neutrophils. on the AD 169 strain (ATCC VR-538), field isolates and a large number Clone CB-gp120 Defensins are relatively resistant to proteolysis, low pH and boiling. of wild strain, immediate early, early and late antigen preparations. Use: IF, E CB-gp120 specifically binds to HIV-1 gp120. The molecule gp120 binds Activation of neutrophils leads to rapid release of HNP. HNP can be The antibody recognizes in Western blot a structural antigen of 21-23 Quantity: 1 ml (MON 2007-1) to overlapping sites involving the CDR2-like region in CD4 domain 1. measured in plasma and other body fluids during infection and kD. This antigen can be detected by fluorescence assay at 96 hours 5 ml (MON 2007-5) Interaction of gp120 with CD4 is crucial for viral infection, but is not inflammation. In normal plasma very low levels of HNP are present. after infection. The antibody gives a specific cytoplasmic perinuclear sufficient to allow viral entry into cells. Source: A BALB/c mouse was Activation of polymorphonuclear leukocytes (PMN) in plasma as occurs fluorescence only in case of late antigen (LA) expression. It does not immunized. After three weeks the mouse was boosted and the during clotting of blood leads to a rapid release of HNP. Anti HNP 1-3 cross react with other herpes viruses. Hepatitis B virus splenocytes fused with myeloma cell line NS0. A positive hybridoma antibody clone D21 recognizes natural HNP 1-3 in biological solutions, obtained after three limiting dilutions served as source for the hybridoma. in tissue sections and leukocyte smears fixed with ethanol, methanol/ Use: F, P, E acetone or paraformaldehyde, in flow cytometry analysis of human Quantity: 1 ml Clone HB3 Cat.no. MON6080 (Monoclonal) Use: F, E neutrophils stained by cell permeabilization method, in Western-Blotting Clone HB5 Cat.no. MON6081 (Monoclonal) Quantity: 100 µg reaction and HNP 1-3 ELISA. Furthermore the antibody is capable to recognize synthetic and recombinant HNP 1-3. These antibodies recognize the following Hepatitis B virus subtypes: ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Use: E, C, F, P, WB Quantity: 100 µg Use: E Also available as BIOTIN conjugate (MON 2062B - 50 µg) Quantity: 100 µg

124 For research use only. Not for drug, diagnostic or other use. www.monosan.com 125 Microbiology / Virology Microbiology / Virology

Klebsiella spp Proteus spp. Respiratory Syncytial Virus, RSV

Cat.no. MON 2006 (Monoclonal) Cat.no. MON 2018 (Monoclonal) Cat.no. MON 8005 (Monoclonal)

Clone 70-2 Clone 31-17 Mixed clones The antibody reacts with a protein determinant in the cell wall of The antibody reacts with approximately 50% of the Proteus spp. For diagnostic purposes a mixture of three monoclonal antibodies Klebsiella. The antibody reacts with 38 of the 77 tested serotypes of isolates tested. directed to different antigens was composed. Two monoclonals are Klebsiella but does not react with several E. coli, P. vulgaris and M. directed against the fusion (F) protein. The third reacts with the nucleo Morganii strains. Positive reactivity was observed with 2 Enterobacter Use: FS (N) protein as determined by immunoprecipitation. The anti out of the 17 tested. The antibody is also reactive with Klebsiella using Quantity: 1 ml (MON 2018-1) F-monoclonals are directed against two different epitopes. Human agglutination. 5 ml (MON 2018-5) respiratory syncytial virus strains A and B as well as bovine virus are recognized. Use: E, A Quantity: 1 ml (MON 2006-1) Pseudomonas aeruginosa Use: F, E 5 ml (MON 2006-5) Quantity: 1 ml

Cat.no. MON 2014 (Monoclonal) Nuclear antigen 1 of Epstein-Barr virus Salmonella serogroup E Clone 69-5 The antibody reacts with flagella of approximately 30% of Pseudomonas Cat.no. MON 6035 (Monoclonal) aeruginosa isolates. Cat.no. MON 2016 (Monoclonal)

Clone E1-2.5 Use: A Clone 42-77 Nuclear antigen 1 of Epstein and Barr virus (EBNA-1). The antibody Quantity: 1 ml (MON 2014-1) The antibody reacts with 9 out of 9 tested Salmonella (belonging to recognizes repetitive gly-ala region of EBNA-1. 5 ml (MON 2014-5) serogroup E). It shows only minor reactivity with a few members of 140 tested non-Salmonella bacteria. No reactivity with other Salmonella Use: E, IC serogroups was observed (40 tested) Quantity: 100 µg Rabies Virus Use: E, A Quantity: 1 ml P55, 50; Epstein Barr Virus Early Antigen Cat.no. MON 7060 (Monoclonal) Clone RAB-50 Staphylococcus aureus peptidoglycan Cat.no. MON 7037 (Monoclonal) Rab-50 reacts with some specific rabies virus strains of vascular type from Eastern Europe. Source: A BALB/c mouse was immunized with Clone 1108-1 rabies virus of the SAD-Vnukovo strain. Splenocytes were fused with Cat.no. MON 2009 (Monoclonal) 1108-1 recognizes a 55;50 kilo Dalton polypeptide associated with NS-0 cells. the early antigen of Epstein-Barr virus (EBV). P55 has been shown to Clone 10-34 be a phosphoprotein and p55;50 has strong DNA-binding activity Use: F, E, B, WB The antibody reacts with several S. aureus peptidoglycan preparations. preferentially to single-stranded DNA. Source: A BALB/c mouse was Quantity: 100 µg The antibody is reactive with some S. epidermis peptidoglycan isolates immunized with immunoprecipitated EBV early antigens. in ELISA. It lacks reactivity with gram negative bacteria.

Use: F, P, IP Lipid A Use: IF, E, A Quantity: 100 µg Quantity: 1 ml (MON 2009-1) 5 ml (MON 2009-5) Cat.no. MON 2012 (Monoclonal) Parvovirus B19 Clone 26-5 Staphylococcus spp The antibody is reactive with Lipid A using ELISA. Lipid A is part of LPS Clone Parvo.3A Cat.no. MON6077 (Monoclonal) (Lipopolysaccaride) present in gram-negative bacteria. Clone Parvo.3B Cat.no. MON6078 (Monoclonal) Cat.no. MON 2008 (Monoclonal) Use: E, A These antibodies react with a 84 kD VP1 capsid protein of B19 human Quantity: 1 ml (MON 2012-1) Clone 17-5 Parvovirus. 5 ml (MON 2012-5) The antibody reacts with several Staphylococcus strains using ELISA. It does not react with several members of the Enterobacteriaceae. Use: WB, IF Quantity: 100 µg Use: IF, E, A Quantity: 1 ml (MON 2008-1) 5 ml (MON 2008-5)

126 www.monosan.com For research use only. Not for drug, diagnostic or other use. 127 Microtubuli Multidrug Resistance Proteins

Alpha-Tubulin Beta III Tubulin MDR1 P-gp (ABCB1) MRP1 (ABCC1)

Cat. no. MON 4018 (Monoclonal) NEW Cat.no. MON 4017 (Monoclonal) Cat.no. MON 9011 (Monoclonal) Cat.no. MON 9017 (Monoclonal)

Clone TU-02 Clone TU-20 Clone JSB-1 Clone MRPm6 TU-02 reacts with alpha-tubulin. Tubulin, the major protein component The antibody reacts with β-III tubulin isoform, peptide sequence (C) The antibody JSB-1 reacts with a conserved cytoplasmic epitope of the MRPm6 reacts with an internal epitope of MRP, a 180-195 kD trans- of microtubel is a heterodimer of two different alpha- and beta-subunits. ESESQPK, which is specific for neurons. The specificity exceeds that of plasma membrane-associated 170-180 kD glycoprotein, the expression membrane transporter protein over expressed in various human Tubulin isotypes have been identified and their expression was shown enolase and as such it can be used as a marker for neural tissues. of which is strongly correlated with the degree of multi-drug-resistance non-P-glycoprotein MDR tumour cell lines. MRPm6 was raised against to be tissue specific. Immunocytochemical studies using mAb revealed Cross reactivity: human and mouse. (MDR) derived MDR cell lines and human MDR cell lines, including cell a bacterial fusion protein of MRP, containing a segment of 170 amino remarkable heterogeneity of tubulin within various nervous tissue. lines derived from lung, ovaries and B-cell lymphomas. Cross reacts with acids in the carboxy terminal end and part of the carboxy proximal Source: TU-02 was raised by the hybridoma technique in mice immunized Use: E, IF, IHC, WB Chinese hamster. No cross reactivity with mouse or rat. nucleotide binding domain of the protein. MRPm6 does not cross react with microtubular protein from pig brain. Quantity: 100 µg with the human MDR1 and MDR3 gene products (Flens et al. 1994) Use: C, WB, F, P Use: F, WB Quantity: 1 ml (MON 9011-1) Use: C, WB, F, P Quantity: 100 µg MAP-2a, MAP-2b (Microtubule-associated 5 ml (MON 9011-5) Quantity: 1 ml (MON 9017-1) 5 ml (MON 9017-5) Proteins) Alpha-Tubulin MDR3 P-gp (ABCB4) Cat.no. MON 5060 (Monoclonal) MRP1 (ABCC1) Cat.no. MON 4012 (Monoclonal) Clone MT-01 Cat.no. MON 9035 (Monoclonal) This antibody reacts with MAP-2a and MAP-2b. Antigen: Thermo stabile Cat.no. MON 9019 (Monoclonal) Clone TU-16 fraction of pig brain microtubules, containing MAP-2 and tau proteins. Clone P3II-26

This antibody TU-16 binds tubulin under both denaturing and non- It cross reacts with human, cat and mouse and probably with many P3II-26 Mab was selected after immunization with a fusion protein Clone MRPm5 denaturing conditions. The antibody reacts with tubulins of various more. The antibody is directed against different antigenic determinant consisting of Gluthathione S Transferase and a fragment of MDR3 P-gp MRPm5 reacts with an internal epitope of MRP1, a 180-195 kD

species ranging from human to plants. in comparison with MT-02. comprising amino acid 629 - 692. P3II-26 reacts with an internal epitope transmembrane transporter protein over expressed in various human

of MDR3 P-gp. P3II-26 does not cross react with the human MDR1 P-gp. non-P-glycoprotein MDR tumour cell lines. MRPm5 was raised against Use: IF, E, IP, WB Use: WB, IC, IP a bacterial fusion protein of MRP1, containing amino acids 986-1204 Quantity: 100 µg Quantity: 100 µg Use: F, WB of the protein. MRPm5 does not cross react with the human MDR1 Quantity: 250 µg and MDR3 gene products.

Alpha-Tubulin, N-terminal structural domain Microtubules Use: F, P, C, WB MRP1 (ABCC1) Quantity: 250 µg Cat.no. MON 4009 (Monoclonal) Cat.no. MON 5090 (Monoclonal) Cat.no. MON 9018 (Monoclonal) MRP2 (ABCC2) Clone TU-01 Clone AE-8 The antibody reacts with alpha-tubulin of various species including plant AE-8 recognizes an antigen associated with cytoplasmic microtubules Clone MRPr1 and yeast tubulins with a N-terminal structural domain of alpha-tubulin. found in human cells. Source: A BALB/c mouse was immunized with cells MRPr1 reacts with an epitope of MRP, a 180-195 kD transmembrane Cat.no. MON 9026 (Monoclonal) This antibody reacts with peptide that corresponds to the position of of the K562 erythroleukemia cell line. The isolated splenocytes were transporter protein over expressed in various human non-P-glycoprotein

alpha/65-79/tubulin. It will react with all alpha-tubulin isoforms. fused with mouse myeloma cells. MDR tumour cell lines. MRPr1 was raised against a bacterial fusion Clone M2I-4

protein of MRP, containing a segment of 168 amino acids in the M2I-4 reacts with an internal epitope of cMOAT/MRP2, a 170-180 kD Use: E, IF, F, WB Use: F, P, C amino-proximal half of the protein. MRPr1 does not cross react with transmembrane protein known as the canalicular multi-organic anion Quantity: 100 µg Quantity: 100 µg the human MDR1 and MDR3 gene products (Flens et al. 1994). transporter, absent in patients with the Dubin-Johnson syndrome, an autosomal recessive liver disorder characterized by chronic conjugated Use: C, WB, F, P hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug Beta-Tubulin, N-terminal structural domain Quantity: 1 ml (MON 9018-1) resistance related protein MRP, and cMOAT/MRP2 over expression has 5 ml (MON 9018-5) been observed in a subset of cisplatin resistant cell lines. M2I-4 was raised against a bacterial fusion protein of cMOAB/MRP2, containing

Cat.no. MON 4010 (Monoclonal) amino acids 215-310 of the protein. M2I-4 did not cross react with the human MDR1, MRP1, MRP3 and MRP5 gene products. Clone TU-06 The antibody reacts with β-tubulin of various species including that of Use: F, WB, P plants. It reacts with the N-terminal structural domain of β-tubulin. Quantity: 250 µg This antibody reacts with all isoforms of β-tubulin.

Use: E, IF, F, WB Quantity: 100 µg

128 www.monosan.com For research use only. Not for drug, diagnostic or other use. 129 Multidrug Resistance Proteins Multidrug Resistance Proteins

MRP2 (ABCC2) MRP2 (ABCC2) MRP4 (ABCC4) MRP5 (ABCC5)

Cat.no. MON 9032 (Monoclonal) Cat.no. MON 9027 (Monoclonal) Cat. no. MON 9057 (Monoclonal) NEW Cat.no. MON 9034 (Monoclonal)

Clone M2II-12 Clone M2III-6 Clone M4I-10 Clone M5II-54

M2II-12 reacts with an internal epitope of cMOAT/MRP2, a 190-200 kD M2III-6 reacts with an internal epitope of cMOAT/MRP2, a 170-180 kD The M4I-10 Mab was selected after immunization with a fusion protein M5II-54 reacts with an internal epitope of MRP5, a 190-200 kD transmembrane protein known as the canalicular multi-organic anion transmembrane protein known as the canalicular multi-organic anion containing the E. coli maltose binding protein and a fragment of the transmembrane protein that is closely related to the multidrug resistance

transporter, absent in patients with the Dubin-Johnson syndrome, an transporter, absent in patients with the Dubin-Johnson syndrome, an human MRP4 protein corresponding to amino acids 372-431. MRP4 protein MRP. M5II-54 was raised against a bacterial fusion protein of autosomal recessive liver disorder characterized by chronic conjugated autosomal recessive liver disorder characterized by chronic conjugated transports cyclic nucleotides and anti-retroviral compounds. The MRP5, containing amino acids 722-910 of the protein. M5I-1 does not

hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug hyperbilirubinemia. cMOAT/MRP2 is closely related to the multidrug M4I-10 Mab also reacts with the mouse orthologue of the transporter cross react with the human MDR1, MRP1, MRP2 or MRP3 gene resistance related protein MRP, and cMOAT/MRP2 over expression has resistance related protein MRP, and cMOAT/MRP2 over expression has molecule (Mrp4). products.

been observed in a subset of cisplatin resistant cell lines. M2II-12 was been observed in a subset of cisplatin resistant cell lines. M2III-6 was raised against a bacterial fusion protein of cMOAB/M-RP2, containing raised against a bacterial fusion protein of cMOAB/MRP2, containing the Use: IC, F, WB Use: F, WB

amino acids 860-950 of the protein. M2II-12 did not cross react with 202-amino acid COOH terminal end of the protein. M2III-6 did not cross Quantity: 150 µg Quantity: 250 µg the human MDR1, MRP1, MRP3 and MRP5 gene products. react with the human MDR1, MRP1, MRP3 and MRP5 gene products.

Use: F, WB Use: F, P, C, WB MRP4 (ABCC4) MRP6 (ABCC6) Quantity: 250 µg Quantity: 250 µg

Cat. no. MON 9069 (Monoclonal) NEW Cat.no. MON 9047 (Monoclonal) MRP2 (ABCC2) MRP3 (ABCC3) Clone M4I-80 Clone M6II-7

The M4I-80 Mab was selected after immunization with a fusion protein M6II-7 reacts with MRP6, a 190-200 kD transmembrane protein that is Cat. no. MON 9056 (Monoclonal) NEW Cat.no. MON 9030 (Monoclonal) containing the E. coli maltose binding protein and a fragment of the related to the multidrug resistance related protein MRP. Mutations in human MRP4 protein corresponding to amino acids 372-431. MRP4 the MRP6 gene are responsible for the connective tissue disorder

Clone M2III-5 Clone M3II-9 transports cyclic nucleotides and anti-retroviral compounds. The Pseudoxanthoma elasticum (PXE). M6II-7 was raised against a bacterial

M2III-5 reacts with an internal epitope of MRP2, a 190-200 kD M3II-9 reacts with an internal epitope of MRP3, a 190-200 kD M4I-80 Mab also reacts with the mouse orthologue of the transporter fusion protein of human MRP6, containing amino acids 764-964, transmembrane protein earlier known as the canalicular multi-organic transmembrane protein that is closely related to the multidrug resistance molecule (Mrp4). spanning the putative 12th transmembrane region as well as predicted

anion transporter cMOAT, absent in patients with the Dubin-Johnson protein MRP1. M3II-9 was raised against a bacterial fusion protein of internal and external regions of the protein. M6II-7 did not cross react

syndrome, an autosomal recessive liver disorder characterized by chronic MRP3, containing amino acids 830-949 of the protein. M3II-9 does not Use: IC, F, WB with the human MDR1, MRP1, MRP2, MRP3, MRP4, MRP5 gene conjugated hyperbilirubinemia. MRP2 is a member of the MRP family cross react with the human MDR1, MRP1, MRP2 or MRP5 gene Quantity: 200 µg products. of multidrug resistance related proteins, and MRP2 overexpression has products.

been observed in a subset of cisplatin resistant cell lines. M2III-5 was Use: F, C, WB raised against a fusion protein of the bacterial maltose binding protein Use: F, WB, C MRP5 (ABCC5) Quantity: 250 µg and rat Mrp2, containing the 202-amino acid COOH terminal end of Quantity: 250 µg the transporter protein. The Mab detects rat, mouse and human MRP2. M III-5 does not cross-react with the human MDR1 P-gp, MRP1, MRP3 Cat.no. MON 9033 (Monoclonal) 2 MRP6 (ABCC6) or MRP5 gene products. MRP3 (ABCC3) Clone M5I-1

Use: F, P, WB, IC, C M5I-1 reacts with an internal epitope of MRP5, a 190-200 kD Cat.no. MON 9048 (Monoclonal) Quantity: 250 µg Cat.no. MON 9031 (Monoclonal) transmembrane protein that is closely related to the multidrug resistance

protein MRP. M5I-1 was raised against a bacterial fusion protein of Clone M6II-21

Clone M3II-21 MRP5, containing amino acids 82-168 of the protein. M5I-1 does not M6II-21 reacts with MRP6, a 190-200 kD transmembrane protein that

M3II-21 reacts with an internal epitope of MRP3, a 190-200 kD cross react with the human MDR1, MRP1, MRP2 or MRP3 gene is related to the multidrug resistance related protein MRP. Mutations transmembrane protein that is closely related to the multidrug resistance products. in the MRP6 gene are responsible for the connective tissue disorder

protein MRP1. M3II-21 was raised against a bacterial fusion protein of Pseudoxanthoma elasticum (PXE). M6II-21 was raised against a bacterial

MRP3, containing amino acids 830-949 of the protein. M3II-21 does not Use: F, WB, C, IC fusion protein of human MRP6, containing amino acids 764-964, cross react with the human MDR1, MRP1, MRP2 or MRP5 gene Quantity: 250 µg spanning the putative 12th transmembrane region as well as predicted

products. internal and external regions of the protein. M6II-21 did not cross react with the human MDR1, MRP1, MRP2, MRP3, MRP4, MRP5 gene Use: F, WB products. Quantity: 250 µg Use: F, C, WB Quantity: 100 µg

130 For research use only. Not for drug, diagnostic or other use. www.monosan.com 131 Multidrug Resistance Proteins Multidrug Resistance Proteins

MRP6 (ABCC6) Breast Cancer Resistance Protein Major Vault Protein (MVP) / Lung Resistance Major Vault Protein (MVP) / Lung Resistance (BCRP, ABCG2), Mouse Protein (LRP) Protein (LRP) Cat.no. MON 9049 (Monoclonal) Cat. nr. MON 9051 (Monoclonal) Cat.no. MON 9028 (Monoclonal) Cat.no. MON 9044 (Monoclonal)

Clone M6II-31

M6II-31 reacts with MRP6, a 190-200 kD transmembrane protein that Clone BXP-53 Clone LMR5 Clone 1014 is related to the multidrug resistance related protein MRP. Mutations The BXP-53 Mab was selected after immunization with a fusion protein LMR5 reacts with an internal epitope of the LRP/Major Vault Protein The mAb 1014 is specific for a 104-kD protein. This mAb is one of four in the MRP6 gene are responsible for the connective tissue disorder containing the E. coli maltose binding protein and a fragment of the (P110), which is strongly over expressed in various human mAb which recognize different epitopes of the protein. This 104-kD

Pseudoxanthoma elasticum (PXE). M6II-31 was raised against a bacterial mouse bcrp protein corresponding to amino acids 221-394. BXP-53 non-P-glycoprotein MDR tumour cell lines. protein is the major vault protein (MVP) also described as the lung fusion protein of human MRP6, containing amino acids 764-964, reacts with an internal epitope of bcrp, a 70 kD transmembrane half- resistance protein (LRP) and has shown to interact with the estrogen spanning the putative 12th transmembrane region as well as predicted transporter which is involved in multidrug resistance. BXP-53 also reacts Use: F, P, C receptor. The protein is part of a very large vault ribonucleoprotein

internal and external regions of the protein. M6II-7 did not cross react with the human BCRP molecule. Quantity: 1 ml complex present in all eukaryotic cells and its structure and protein with the human MDR1, MRP1, MRP2, MRP3, MRP4, MRP5 gene composition is highly conserved. Because of the size, shape, and protein products. Use: F, WB, C and RNA composition of this complex the particles are different from Quantity: 1 ml Major Vault Protein (MVP) / Lung Resistance other ribonucleoproteins. Source: A BALB/c mouse was immunized Use: F, P, C, WB with 5 µg of affinity purified nuclear extract proteins. Spleen cells were Quantity: 50 µg Protein (LRP) fused with equal number of Sp0Ag-14 myeloma cells. Cells whose Breast Cancer Resistance Protein supernatant showed a positive signal for a 104-kD band were selected Cat.no. MON 9036 (Monoclonal) and cloned by limited dilution. (BCRP, ABCG2), Mouse Breast Cancer Resistance Protein Clone MVP-37 Use: F, WB (BCRP,ABCG2) Cat. no. MON 9050 (Monoclonal) MVP-37 reacts with an internal epitope of MVP/LRP (p110), which is Quantity: 100 µg strongly over expressed in various human non-P-glycoprotein MDR Cat.no. MON 9037 (Monoclonal) Clone BXP-9 tumour cell lines, accordingly to an increase in the number of vault The BXP-9 Mab was selected after immunization with a fusion protein particles. Major Vault Protein (MVP) / Lung Resistance Clone BXP-34 containing the E. coli maltose binding protein and a fragment of the BXP-34 Mab was selected after immunization with the mitoxanthrone mouse bcrp protein corresponding to amino acids 221-394. BXP-9 Use: F, P, WB Protein (LRP) resistant, BCRP overexpressing cell line MCF7 MR. BXP-34 reacts with reacts with an internal epitope of bcrp, a 70 kD transmembrane half- Quantity: 250 µg an internal epitope of BCRP, a 70 kD transmembrane half-transporter transporter which is involved in multidrug resistance. BXP-9 does not Cat.no. MON 9045 (Monoclonal) which is involved in multidrug resistance. BXP-34 did not cross react react with the human BCRP molecule. with the human MDR1, MRP1, MRP2, MRP5 gene products. Major Vault Protein (MVP) / Lung Resistance Clone 1027 Use: F, WB, C The mAb 1027 is specific for a 104-kD protein. This mAb is one of four Use: F, P, C Quantity: 1 ml Protein (LRP) mAb which recognize different epitopes of the protein. This 104-kD Quantity: 250 µg protein is the major vault protein (MVP) also described as the lung Cat.no. MON 9043 (Monoclonal) resistance protein (LRP) and has shown to interact with the estrogen Major Vault Protein (MVP) / Lung Resistance receptor. The protein is part of a very large vault ribonucleoprotein Breast Cancer Resistance Protein Clone 1011 complex present in all eukaryotic cells and its structure and protein Protein (LRP) The mAb 1011 is specific for a 104-kD protein. This mAb is one of four composition is highly conserved. Because of the size, shape, and protein (BCRP,ABCG2) mAb which recognize different epitopes of the protein. This 104-kD and RNA composition of this complex the particles are different from Cat.no. MON 9016 (Monoclonal) protein is the major vault protein and has shown to interact with the other ribonucleoproteins. Source: A BALB/c mouse was immunized Cat.no. MON 9041 (Monoclonal) estrogen receptor The protein is part of a very large vault ribonucleo- with 5 µg of affinity purified nuclear extract proteins. Spleen cells were Clone LRP-56 protein complex present in all eukaryotic cells and its structure and fused with equal number of Sp0Ag-14 myeloma cells. Cells whose Clone BXP-21 LRP-56 reacts with an internal epitope of the LRP-protein (P110), protein composition is highly conserved. Because of the size, shape, supernatant showed a positive signal for a 104-kD band were selected BXP-21 Mab was selected after immunization with a fusion protein which is strongly over expressed in various human non-P-glycoprotein and protein and RNA composition of this complex the particles are and cloned by limited dilution. consisting of the E. coli maltose-binding protein and a 126 amino acids MDR tumour cell lines. different from other ribonucleoproteins. Source: A BALB/c mouse was part of the BCRP peptide (aa 271-396 of BCRP (GenBank accession no. immunized with 5 µg of affinity purified nuclear extract proteins. Spleen Use: F, WB AF098951)). BXP-21 reacts with an internal epitope of BCRP, a 70 kD Use: C, F, P cells were fused with equal number of Sp0Ag-14 myeloma cells. Cells Quantity: 100 µg transmembrane half-transporter which is involved in multidrug Quantity: 100 µg whose supernatant showed a positive signal for a 104-kD band were resistance. BXP-21 did not cross react with the human MDR1, MRP1, selected and cloned by limited dilution. MRP2 gene products. Use: F, WB Use: F, P, WB, C Quantity: 100 µg Quantity: 250 µg

132 www.monosan.com For research use only. Not for drug, diagnostic or other use. 133 TitelMultidrug Hoofdstuk Resistance Proteins Muscles

Major Vault Protein (MVP) / Lung Resistance VPARP antibody to the minor vault protein Actin Myosin Protein (LRP) Cat.no. MON 9040 (Monoclonal) Cat.no. MON 4001 (Monoclonal) Cat.no. MON 4005 (Monoclonal) Cat.no. MON 9046 (Monoclonal) Clone P193-6 Clone 5F9F5 Clone 414 Clone 1032 P193-6 reacts with an internal epitope (amino acids 593-599, FSKVEDY) This antibody is highly reactive with filamentous actin. Cross reactivity This monoclonal antibody reacts in immunoblots specific with myosin The mAb 1032 is specific for a 104-kD protein. This mAb is one of four of the minor vault protein (p193 or VPARP), which is over expressed in with beta,-gamma- and delta- actin occurs. No cross reactivity can be and is specific to the 75kD of Myosin. Not specific for alpha-cardiac mAb which recognize different epitopes of the protein. This 104-kD various human non-P-glycoprotein MDR tumour cell lines, accordingly detected with the other components of the cytoskeleton. myosin. Does not react with the light-chain but with the last 75 kD of protein is the major vault protein (MVP) also described as the lung to an increase in the number of vault particles. p193-6 was raised the myosin molecule. These 75 kD are located on a myosin-common resistance protein (LRP) and has shown to interact with the estrogen against an E. coli lysate transformed with the pET28a(+) expression Use: F site-chain. receptor. The protein is part of a very large vault ribonucleoprotein vector containing amino acids 408-611 of the p193 cDNA. Quantity: 1 ml (MON 4001-1) complex present in all eukaryotic cells and its structure and protein 5 ml (MON 4001-5) Use: F, P, WB composition is highly conserved. Because of the size, shape, and protein Use: F, WB Quantity: 1 ml and RNA composition of this complex the particles are different from Quantity: 250 µg other ribonucleoproteins. Source: A BALB/c mouse was immunized Actin, Chicken with 5 µg of affinity purified nuclear extract proteins. Spleen cells were Smoothelin fused with equal number of Sp0Ag-14 myeloma cells. Cells whose Mouse IgG1 negative control supernatant showed a positive signal for a 104-kD band were selected Cat.no. MON 4002 (Monoclonal) and cloned by limited dilution. Cat. no. MON 3093 (Monoclonal) NEW Cat.no. MON 9011C (Monoclonal) Clone 647 Use: F, WB This antibody reacts in immunoblots with one single protein band which Clone C6G Quantity: 100 µg Clone CT6 is corresponding to actin. Antigen origin: cell preparation of chicken Smoothelin is one of the smooth muscle cell specific proteins such as The antibody reacts with a guinea pig lymphocyte subset probably skeletal muscle. Antigen location: cytoplasm. calponin, alpha smooth muscle cell actin, SM22alpha and smooth muscle analog to human CD8 (cytotoxic/suppressor) subset. myosin heavy chain isoforms. Smoothelin is exclusively expressed in VPARP antibody to the minor vault protein Use: F fully differentiated (contractile) SMCs. It colocalizes with actin stress Use: C, WB, F, P Quantity: 1 ml fibers, this distribution indicates a correlation between smoothelin Quantity: 1 ml expression and smooth muscle tissue contractility. Two major isoforms Cat.no. MON 9038 (Monoclonal) of smoothelin have been reported, a 59-kDa smoothelin-A in visceral Desmin smooth muscle cells and a 110-kDa smoothelin-B in vascular smooth Clone P193-10 MDR Sampler Pack muscle cells. Both gene products are encoded by a single copy gene P193-10 reacts with an internal epitope (amino acids 506-510, VALGK) which is located on chromosome 22 and regulated by a dual promoter of the minor vault protein (p193 or VPARP), which is over expressed in Cat.no. MON 3001 (Monoclonal) system in a tissue specific manner. The variation in the smoothelin various human non-P-glycoprotein MDR tumour cell lines, accordingly Cat.no. MON 9200 (Monoclonal) proteins is achieved by alternative splicing in the calponin homology to an increase in the number of vault particles. P193-10 was raised Clone D33 domain. Antibodies to Smoothelin are powerful tool in the evaluation of against an E. coli lysate transformed with the pET28a(+) expression Clone JSB-1, LRP56, MRPm6 and MRPr1 This antibody is highly reactive with desmin. On immunoblots only the smooth muscle cell differentiatiation as occurs in atherosclerotic lesions. vector containing amino acids 408-611 of the p193 cDNA. This sampler pack contains the regular products: MON 9011, MON 56 kD desmin band is stained. Immunogen derived from human 9016, MON 9017 and MON 9018. JSB-1 (ab-1) has potential value for Leiomyoma. Use: WB, C, F Use: F, WB diagnostics detection of MDR cells in human tumour samples. LRP-56 Quantity: 100 µg Quantity: 250 µg (ab-2) has potential value for detection of LRP-associated non Pgp MDR Use: F, P, WB in human tumour samples. MRPm6 (ab-3) and MRPr1 (ab-4) have Quantity: 1 ml (MON 3001-1) potential value for detection of MRP-related non-Pgp MDR in human 5 ml (MON 3001-5) Smoothelin VPARP antibody to the minor vault protein tumour samples. For more information look at individual data sheets. Use: C, F, P Desmin Cat.no. MON 2094 (Monoclonal) Cat.no. MON 9039 (Monoclonal) Quantity: 0.25 ml of each Clone R4A Clone P193-4 Cat.no. PS 031 (Polyclonal) A cytoskeletal protein exclusively found in smooth muscle cells. Reacts P193-4 reacts with an internal epitope (amino acids 491-494, HPGE) with the 59 kDa and 100 kDa protein corresponding to smoothelin. of the minor vault protein (p193 or VPARP), which is over expressed in The antiserum is specifically directed against 53 kDa Desmin protein, Cross reactivity is observed with human, hamster, canine, cat, chicken, various human non-P-glycoprotein MDR tumour cell lines, accordingly the main intermediate filament in mature skeletal, cardiac and smooth swine and monkey. to an increase in the number of vault particles. p193-4 was raised muscle cells. Cross reactivity is observed with Desmin from most against an E. coli lysate transformed with the pET28a(+) expression mammals (also mouse and rat) and birds. Immunogen: preparation from Use: F, P, WB vector containing amino acids 408-611 of the p193 cDNA. chicken gizzard. Antigen location, cytoplasm. Quantity: 1 ml

Use: F, WB Use: F, P Quantity: 250 µg Quantity: 0.25 ml

134 For research use only. Not for drug, diagnostic or other use. www.monosan.com 135 Muscles Neuroscience

Vimentin Alpha-Fetoprotein Alpha-B-crystallin

Cat.no. MON 3005 (Monoclonal) Cat.no. MON 5032A (Monoclonal) Cat.no. PS 094 (Polyclonal)

Clone V9 Clone AFP-01 This polyclonal antibody reacts with the 23 kDa alpha-B-crystallin protein. Vimentin is the major subunit protein of the intermediate filaments of The antibody is highly specific for human alpha-fetoprotein. No cross This highly immunogenic protein has shown to have some homology mesenchumal cells, and has a molecular weight of 57 kD. Immuno- reactions were found with other serum proteins. The epitope is with small heat shock proteins. A single protein fraction derived form histochemical staining for Vimentin is thus characteristic of sarcomas dependent on Ca2+ (sensitive to EDTA). myelin form Multiple Sclerosis (MS)-affected brain tissue showed to (of neural, muscle and fibroblast origin) compared to carcinomas which invoke a dominant respons on T-cells from MS patients as well as are generally negative. Melanomas, lymphomas and vascular tumours Use: E healthy controls. Findings indicate that alpha-B-crystallin serves as may all stain for Vimentin. Vimentin antibodies are thus of value in the Quantity: 100 µg immunodominant myelin antigen to human T-cells when expressed at differential diagnosis of malignant tumours, generally used with a the elevated levels found in active MS lesions. The immunogen has panel of other antibodies including those recognizing cytokeratins, been derived from bovine eye lens and differs only in four of the 175 lymphoid markers, S100, desmin and neurofilaments. The antibody Alpha-Fetoprotein amino acid positions with the human protein. stains the 57 kD vimentin band in immunoblots performed on a lysate of normal human lymphocytes. It does not stain mouse or rat vimentin. Use: P, WB, E Cat.no. MON 5032B (Monoclonal) Quantity: 1 ml Use: F, P, WB Quantity: 1 ml (MON 3005-1) Clone AFP-11 5 ml (MON 3005-5) The antibody is highly specific for human alpha-fetoprotein. No cross Capsaicin receptor reactions were found with other serum proteins.

Vimentin Use: E Cat.no. PS 264 (Polyclonal) Quantity: 100 µg Immunogen: Synthetic peptide from the C-terminal of the capsaicin Cat. no. MON 2091 (Monoclonal) NEW receptor conjugated to BSA. Works on rat tissues. Positive control: Alpha-B-crystallin Frozen sections of spinal cord from rat. Absorption with 10-100 µg Clone VI-RE/1 immunogen per ml diluted antiserum abolishes the staining. The antibody reacts with human vimentin. Cat.no. MON 4015 (Monoclonal) Use: F Use: WB, IC, E Quantity: 50 µl Quantity: 100 µg Clone JAM 07 This monoclonal antibody reacts with the 23 kDa alpha-B-crystallin protein. This highly immunogenic protein has shown to have some Galanin homology with small heat shock proteins. A single protein fraction derived from myelin from Multiple Sclerosis (MS)-affected brain tissue showed to invoke a dominant respons on T-cells from MS patients as Cat.no. PS 228 (Polyclonal) well as healthy controls. Findings indicate that alpha-B-crystallin serves as immunodominant myelin antigen to human T-cells when expressed Immunogen: Synthetic galanin 1-29. Tested on human and rat tissues. at the elevated levels found in active MS lesions. The immunogen has Positive control: Bouin-fixed paraffin or Stefanini-fixed frozen sections been derived from bovine eye lens and differs only in four of the 175 of rat colon. Absorption with 10-100 µg galanin 1-29 per ml diluted amino acid positions with the human protein. antiserum abolishes the staining, while galanin 1-10, VIP, NPY and SP do not. Use: WB, E Quantity: 1 ml (MON 4015-1) Use: P, F 5 ml (MON 4015-5) Quantity: 50 µl

GAP-43

Cat.no. PS 236 (Polyclonal)

Immunogen: Synthetic GAP-43 (211-226), conjugated to BSA. Tested on rat tissues. Positive control: Stefanini-fixed frozen sections of rat duodenum. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.

Use: F Quantity: 50 µl

136 For research use only. Not for drug, diagnostic or other use. www.monosan.com 137 Neuroscience Neuroscience

Gastrin GFAP (Glial Fibrillary Acid Protein) Myelin Neurofilament

Cat.no. PS 250 (Polyclonal) Cat.no. PS032 (Polyclonal) Cat.no. MON 4004 (Monoclonal) Cat.no. MON 2098 (Monoclonal)

Immunogen: Synthetic human gastrin I, conjugated to BSA. Tested on The antiserum is specifically directed against the 56 kDa Glial Fibrillary Clone 2B5 Clone RNF406 rat tissues. Positive control: Formalin-fixed paraffin sections of rat antrum. Acid Protein (GFAP). This is the main subunit of intermediate filaments This monoclonal antibody reacts exclusively with myelin sheaths. Reacts with intermediate filament protein in peripheral and central nerve Absorption with 10-100 µg gastrin 1-34 and CCK 8 per ml diluted of glial cells and astrocytes. Immunogen: isolated from human spinal The antibody is directed against a 43 kDa protein. cells. In Western blotting reactive only with the 160 kDa protein. Reacts antiserum abolishes the staining. cord. Antigen location, cytoplasm. Cross reactivity is observed with GFAP with phosphorylated isoform. Cross reactivity is observed with human. in bovine and rat tissue. Use: F, P Use: P, F Quantity: 1 ml Use: F, WB Quantity: 50 µl Use: F, P Quantity: 1 ml Quantity: 0.25 ml NKA/SK GFAP (Glial Fibrillary Acid Protein) Neurofilament Histidine Decarboxylase Cat.no. PS 232 (Polyclonal) Cat.no. MON 3002 (Monoclonal) Cat.no. MON 2099 (Monoclonal) Cat.no. PS 260 (Polyclonal) Immunogen: Neurokinin A/Substance K, conjugated to BSA. Tested on Clone 6F2 rat tissues. Positive control: Frozen sections of rat colon. Absorption Clone RNF403 The antibody stains cells containing GFAP. The antibody only stains the Immunogen: Recombinant histidine decarboxylase produced in E. coli. with 10-100 µg NKA per ml diluted antiserum abolishes the staining, Reacts with intermediate filament protein of nerve cells corresponding 56kD GFAP band in immunoblots performed on crude brain extract. It Tested on human, rat, mouse, dog and guinea-pig tissues. Positive while SP does not. in Western blotting assays with the 160 kDa neurofilament protein. reacts also with mouse GFAP. control: Stefanini-fixed frozen sections of fundus from rat. Absorption Reacts with phosphorylated isoform. Cross reactivity is observed with with 10-100 µg immunogen per ml diluted antiserum abolishes the Use: P, F human. Use: F, P, IF staining. Quantity: 50 µl Quantity: 1 ml (MON 3002-1) Use: F, WB 5 ml (MON 3002-5) Use: P, F Quantity: 1 ml Quantity: 50 µl Neurofilament GFAP (Glial Fibrillary Acid Protein) Neurofilament Kinesin Cat.no. MON 3031 (Monoclonal) Cat.no. MON 3012 (Monoclonal) Clone 403 Cat.no. MON 3011 (Monoclonal) Cat.no. MON 4046 (Monoclonal) The monoclonal antibody reacts specific with the medium component Clone GF-01 of the three subunits of neurofilaments. In immunoblots it reacts with Cone NF-01 The monoclonal antibody reacts with GFAP, a 52 kD intermediate Clone KN-01 the 160 kD protein. Neurofilaments, the intermediate filaments of The monoclonal antibody reacts specific with the heavy component of filament protein, which is expressed by astrocytes and to a lesser degree The antibody reacts with conventional kinesin associated with vesicles neurons, are composed of three polypeptides with a molecule weight the three subunits of neurofilaments. In immunoblots it reacts with the by ependymal cells, folliculostellate cells of the anterior pituary and and with lower affinity with denaturated molecule. Recognizes the of respectively, 70, 160 and 200 kD. The three polypeptides can be 210 kD protein. The antibody does not cross react with other intermediate cerebellar radial glia. The antibody does not react with other intermediate heavy chain of Kinesin. Positive species cross reactivity was observed differentially expressed during neuronal development, or in adult brain proteins. It reacts also with porcine neurofilaments. filaments. The antibody reacts also with porcine GFAP, negative species: in human, mouse and pig. tissue. Antibodies directed against the three different neurofilament nouse, rat. proteins can be an important aid in studying neurofilament expression Use: F, P, WB Use: IC, E, WB pattern and in testing of tumours. Antigen origin: neurofilament Quantity: 100 µg Use: F, P, WB, E Quantity: 100 µg preparation of human spinal cord. Antigen location: cytoplasma. Quantity: 100 µg Use: F, P Motilin Quantity: 1 ml GFAP (Glial Fibrillary Acid Protein) Cat.no. PS 208 (Polyclonal) Neurofilament Cat.no. MON 3028 (Monoclonal) Pure porcine motilin, conjugated to BSA. Tested on human, pig and cat Clone GFA-02 tissues. Does not work on rat tissues. Positive control: Formalin-fixed Cat.no. MON 3004 (Monoclonal) GFA-02 reacts with Glial Fibrillary Acid Protein (52 kD). GFAP is the major paraffin sections of pig duodenum. Absorption with 10-100 µg protein of glial filaments in astrocytes and ependymal cells. In the immunogen per ml diluted antiserum abolishes the staining. Clone 2F11 peripheral nervous system GFAP is found in Schwann cells. Source: This antibody stains the 70 kD and 200 kD polypeptides of neurofilament. A BALB/c mouse immunized with a crude preparation from porcine Use: P, F No reactive with small cell lung carcinoma. spinal cord. Quantity: 50 µl Use: F, WB, P Use: F, P, C, WB Quantity: 1 ml (MON 3004-1) Quantity: 100 µg 5 ml (MON 3004-5)

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Neurofilament Neurotensin Nitrotyrosine NPY

Cat.no. MON 3030 (Monoclonal) Cat.no. PS 224 (Polyclonal) Cat.no. MON 9029 (Monoclonal) Cat.no. PS 227 (Polyclonal) Immunogen: Synthetic bovine neurotensin, conjugated to BSA. Tested Clone 402 on cat tissues. Positive control: Bouin-fixed paraffin or Stefanini-fixed Clone HM11 Immunogen: Synthetic neuropeptide Y (NPY), conjugated to BSA. Tested The monoclonal antibody reacts specific with the heavy component of frozen sections of cat ileum. Absorption with 10-100 µg neurotensin This highly specific monoclonal antibody reacts with nitrotyrosine, both on human, rat and guinea-pig tissues. Positive control: Stefanini-fixed the three subunits of neurofilaments. In immunoblots it reacts with the per ml diluted antiserum abolishes the staining while glucagon, insulin, with the free amino acid as well as with proteins containing nitrotyrosine. frozen sections of rat small intestine. Absorption with 10-100 µg NPY 200 kD protein. Neurofilaments, the intermediate filaments of neurons, gastrin, GIP and VIP do not. The presence of nitrotyrosine has been detected in various inflammatory per ml diluted antiserum abolishes the staining while PYY and PP do not. are composed of three polypeptides with a molecule weight of processes including atherosclerotic plaques. Nitrotyrosine is formed in respectively, 70, 160 and 200 kD. The three polypeptides can be Use: P, F tissues in presence of the active metabolite NO. Various pathways Use: P, F differentially expressed during neuronal development, or in adult brain Quantity: 50 µl including the formation of peroxinitrite lead to nitrotyrosine production. Quantity: 50 µl tissue. Antibodies directed against the three different neurofilament proteins can be an important aid in studying neurofilament expression Use: P, F, WB pattern and in testing of tumours. Antigen origin: isolated from calf brain Neuro Specific Enolase, NSE, gamma-gamma Quantity: 100 µg NPY 1-20 tissue. Antigen location: cytoplasma. Also available as BIOTIN conjugate (MON 9029B - 50 µg)

Use: F, P Cat.no. MON 9004 (Monoclonal) Cat.no. PS 231 (Polyclonal) Quantity: 1 ml NO-synthase Clone MIG-N3 Immunogen: Synthetic cys(21)-NPY 1-21, conjugated to BSA. Tested on The antibody binds to human gamma-gamma enolase and to material rat tissues. Positive control: Stefanini-fixed cryostat sections of rat Neuromedin U-8 from guinea pigs; does not bind to serum protein A. Specificity of the Cat.no. PS 243 (Polyclonal) small intestine. Absorption with 10-100 µg NPY 1-20 per ml diluted monoclonal has been characterized by enzyme immunoassay and antiserum abolishes the staining while PYY and PP do not. immunohistological staining. The antibody was also tested using Western Immunogen: Synthetic peptide from the C-terminal of the cloned rat Cat.no. PS 206 (Polyclonal) blotting analysis of different isoenzymes. The reagent is evaluated on cerebellar NO-synthase, coupled to BSA. Tested on human and rat tissues. Use: F several tissues known to be positive or negative for the antigen. Positive control: Frozen sections of rat colon. Absorption with 10-100 µg Quantity: 50 µl Synthetic neuromedin U-8, porcine sequence, conjugated to BSA. Immunogen: purified human gamma-gamma enolase. immunogen per ml diluted antiserum abolishes the staining. Tested on chicken and rat tissues. Positive control: Frozen sections of chicken small intestine. Absorption with 10-100 µg immunogen per ml Use: P, WB Use: F NSP-A and NSP-B diluted antiserum abolishes the staining. Quantity: 0.5 ml Quantity: 50 µl

Use: F Cat.no. MON 2095 (Monoclonal) Quantity: 50 µl Nitric oxide synthase Rat NO-synthase Clone RNL-2 Recognizing epitopes in the neuro-endocrine specific proteins (NSP) A Neurotensin Cat.no. PS 059 (Polyclonal) Cat.no. PS 257 (Polyclonal) and B. This antibody was raised against the small cell lung cancer cell line NCI-H82. Cross reactivity is observed with human and rat. Nitric oxide synthase is an enzyme catalysing the synthesis of NO from Immunogen: Synthetic peptide from the C-terminal of the cloned rat Cat.no. PS 090 (Polyclonal) L-arginine. This antiserum was raised against a synthetic fragment of cerebellar NO-synthase, coupled to BSA. Tested on human and rat tissues. Use: F, WB rat cerebellar NO-synthase, that shows no homology with other related Positive control: Frozen sections of rat colon or small intestine. Absorption Quantity: 1 ml Neurotensin is localized in neurons within the hypothaloamus and in proteins. The antigen used for immunization was synthetic peptide from with 10-100 µg immunogen per ml diluted antiserum abolishes the endocrine cells of the ileum medulla and the adrenal medulla. The the C-terminal of cloned rat cerebellar NO-synthase, coupled to BSA. staining. neurotensin antibody can be used to discriminate tumours derived The antibody reacts in rat and human frozen tissue. Absorption with NSP-A and NSP-B from these cells. Immunogen: synthetic bovine neurotensin. Antigen 10-100 µg immunogen per ml diluted antiserum abolishes the staining. Use: F location, cytoplasm. Cross reactive with human, rat and cat. Quantity: 50 µl Use: IF, F Cat.no. MON 2096 (Monoclonal) Use: F, P Quantity: 50 µl Quantity: 0.25 ml NO-synthase Clone RNL-3 Recognizing epitopes in the neuro-endocrine specific proteins (NSP) A and B. This antibody was raised against the small cell lung cancer cell Cat.no. PS 258 (Polyclonal) line NCI-H82. Cross reactivity is observed with human and rat.

Immunogen: Synthetic peptide from the N-terminal of the cloned rat Use: F, WB cerebellar NO-synthase, coupled to polylysine. Tested on human and rat Quantity: 1 ml tissues. Positive control: Frozen sections of rat colon. Absorption with 10-100 µg immunogen per ml diluted antiserum abolishes the staining.

Use: F Quantity: 50 µl

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NSP-C Substance P Synaptophysin, Rat Vesicular acethylcholine transporter (VAChT)

Cat.no. MON 2097 (Monoclonal) Cat.no. PS 093 (Polyclonal) Cat.no. MON 9013 (Monoclonal) Cat.no. PS 212 (Polyclonal)

Clone RNL-4 Substance P occurs in nerve fibres of the central and peripheral nervous Clone SY38 Synthetic peptide from the C-terminal of human VAChT conjugated to Recognizing epitopes in the neuro-endocrine specific proteins (NSP) C. system and in endocrine cells of the gut. Substance P containing nerve The antibody is specific for Synaptophysin. The specificity was ascertained BSA. Works on human tissues. Positive control: Frozen sections of human This antibody was raised against the amino-terminal amino acid fibres in the gut wall are particularly numerous in the myenteric plexus by immunoblotting and immunohistochemistry. The antibody small intestine. Absorption with 10-100 µg immunogen per ml diluted sequence of NSP-C. Cross reactivity is observed with human and rat. and the smooth muscle layers. Substance P containing tumours arising predominantly reacts with a 38 kDa transmembrane glycoprotein from antiserum abolishes the staining. in the ileum are often associated with the “carcinoid syndrome”, synaptic vesicles. Antigen was isolated from synaptic vesicles. Antigen Use: F, P, WB characterized by flushing of the skin, diarrhoea, bronchoconstriction location, cytoplasm. Cross reactivity with rat, mouse and cow. Use: F Quantity: 1 ml and sudden drops in blood pressure. Substance P is commonly found Quantity: 50 µl in midgut carcinoids and some of the symptoms may be related to this Use: F, P peptide. Quantity: 1 ml Ornithine decarboxylase Vesicular acethylcholine transporter Use: F (VAChT), Rat Quantity: 0.25 ml Tangles Cat.no. PS 259 (Polyclonal) Cat.no. PS 246 (Polyclonal) Immunogen: Recombinant human ODC produced in E. coli. Tested on Substance P, Rat Cat.no. MON 6003 (Monoclonal) mouse tissues. Positive control: Stefanini-fixed frozen sections of renal Immunogen: Synthetic peptide from the C-terminal of rat VAChT cortex from testosterone-treated mice. Absorption with 10-100 µg Clone MIG-T4 conjugated to BSA. Works on rat and mouse tissues. Positive control: immunogen per ml diluted antiserum abolishes the staining. Cat.no. PS 210 (Polyclonal) This antibody binds to human neurofibrillary tangles. Specificity of the Frozen sections of rat small intestine. Absorption with 10-100 µg monoclonal has been characterized by enzyme immunoassay and immunogen per ml diluted antiserum abolishes the staining. Use: F Synthetic SP 4-11, conjugated to thyroglobulin using CDI. Tested on rat immunohistological staining. The reagent was evaluated on several Quantity: 50 µl tissues. Positive control: Frozen sections of rat colon. Absorption with tissues known to be positive or negative for the antigen. No cross Use: F 10-100 µg SP 4-11 per ml diluted antiserum abolishes the staining. reactivity has been observed. Quantity: 50 µl

Serotonin Use: P, F Use: P Quantity: 50 µl Quantity: 1.5 ml Vesicular monoamine transporter (VMAT2) Cat.no. PS 010 (Polyclonal) Substance P, Rat Vesicular GABA transporter (VGAT), Rat Cat.no. PS 213 (Polyclonal) The antibody is specific for serotonin. No cross reactivity consists with other monoamines such as various tryptamine-derivates or catecho- Synthetic peptide from the C-terminal of rat VMAT2 conjugated to lamines such as dopamine, noradrenalin or adrenaline. Cat.no. PS 225 (Polyclonal) Cat.no. PS 237 (Polyclonal) BSA. Tested on human, rat, mouse and pig tissues. Positive control: Frozen sections of rat small intestine. Absorption with 10-100 µg Use: IF Immunogen: Substance P, conjugated to BSA. Tested on rat tissues. Immunogen: Synthetic peptide from the C-terminal of VGAT conjugated immunogen per ml diluted antiserum abolishes the staining. Quantity: 50 µl Positive control: Frozen sections of rat colon. Absorption with 10-100 µg to BSA. Works on rat tissues. Positive control: Frozen sections of SP per ml diluted antiserum abolishes the staining while neurokinin A cerebellum from rat. Absorption with 10-100 µg immunogen per ml Use: F does not. diluted antiserum abolishes the staining. Quantity: 50 µl Serotonin (5-HT) Use: P, F Use: F Quantity: 50 µl Quantity: 50 µl Vesicular monoamine transporter (VMAT2) Cat.no. PS233 (Polyclonal)

Serotinin is produced by endocrine cells of the stomach, duodenum Substance P, Rat Vesicular acethylcholine transporter Cat.no. PS 247 (Polyclonal) and ileum. The serotonin antibody can be used to differentiate tumours of serotoninergic origin (VAChT), Rat Immunogen: Synthetic peptide from the C-terminal of rat VMAT2 Cat.no. PS 251 (Polyclonal) conjugated to BSA. Tested on human, rat, mouse and pig tissues. Positive Use: P, F Cat.no. PS 211 (Polyclonal) control: Frozen sections of rat small intestine. Absorption with 10-100 µg Quantity: 250 µl Immunogen: Substance P, conjugated to BSA. Tested on rat tissues. immunogen per ml diluted antiserum abolishes the staining. Positive control: Cryostat sections of rat colon. Absorption with 10-100 µg Synthetic peptide from the C-terminal of rat VAChT conjugated to BSA. SP and NKA per ml diluted antiserum abolishes the staining while GRP Works on rat and mouse tissues. Positive control: Frozen sections of rat Use: F and NKB do not. small intestine. Absorption with 10-100 µg immunogen per ml diluted Quantity: 50 µl antiserum abolishes the staining. Use: F Quantity: 50 µl Use: F, P Quantity: 50 µl

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VIP, Rat Albumin Amyloid A

Cat.no. PS 218 (Polyclonal) Cat.no. MON 5024 (Monoclonal) Cat.no. MON 5065 (Monoclonal)

Immunogen: Pure porcine VIP. Tested on cat and rat tissues. Positive Clone A1-01 Clone Reu-86.2 control: Formalin-fixed paraffin sections of cat ileum. Frozen sections The antibody is highly specific for human albumin. No cross reactions The antibody reacts with AA-type Amyloid depositions. Amyloidosis of rat small intestine. Absorption with 10-100 µg immunogen per ml were found with other serum proteins. The antibody does not cross react includes a family of diseases which have in common the extra cellular diluted antiserum abolishes the staining, while secretin, PHI, glucagon, with bovine, porcine rabbit and mouse serum albumin. deposition of β-pleated fibrillar protein. Amyloidosis associated with GIP and CCK do not. chronic inflammatory conditions (AA-type) and Amyloidosis related to Use: E, WB plasma cell dyscrasia (AL-type) are the most common, but the frequency Use: P, F Quantity: 100 µg of amyloidosis associated with chronic haemodialysis (B2M-type) is Quantity: 50 µl growing rapidly. Reu-86.2 can be used in routine diagnosis of amyloidosis both for detection of the AA-type as for the discrimination Alpha-1-Antitrypsin from the other types of amyloidosis such as the B2M- and AL-type. VIP Antigen origin: human serum Amyloid A (Apo-SAA). Antigen location: extra cellular matrix. Cat.no. MON 5037 (Monoclonal) Cat.no. PS 249 (Polyclonal) Use: F, P Clone F46.4.1. Quantity: 1 ml Immunogen: Synthetic human VIP. Tested on human, pig, rat and The antibody, raised by immunization with a synthetic peptide, reacts

guinea-pig tissues. Positive control: Stefanini-fixed frozen sections of with human α1-antitrypsin, both the M and the Z variants, in ELISA. rat intestine. Absorption with 10-100 µg VIP per ml diluted antiserum Amyloid P abolishes the staining, while PHI does not. Use: E, WB Quantity: 1 ml Use: P, F Cat.no. MON 6006 (Monoclonal) Quantity: 50 µl Alpha-1-Antitrypsin-Z Clone 5.4A This antibody can be used as a capture antibody in ELISA for the determination of human serum amyloid P (SAP) which is believed to Cat.no. MON 5038 (Monoclonal) be intimately linked to β-amyloid in amyloid deposits. The amyloid deposits themselves are involved in the development of the dementia Clone F50.4.1. characteristics of Alzheimer disease, hence the opportunity for further The antibody, raised by immunization with a synthetic peptide, reacts study of the involvement of SAP in this degenerative disease. This

with human α1-antitrypsin-Z in ELISA. The antibody does not react with antibody is recommended together with the biotinylated antibody

other variants of α1-antitrypsin. MON 6007 for use in a capture ELISA.

Use: E, WB Use: E Quantity: 1 ml Quantity: 250 µg

Alpha-1-Antitrypsin, non-Z Amyloid P, Biotin conjugated

Cat.no. MON 5039 (Monoclonal) Cat.no. MON 6007 (Monoclonal)

Clone F43.8.1. Clone 5.4D.3B

The antibody, raised with a synthetic peptide, reacts with human α1- This biotinylated antibody can be used as a capture antibody in ELISA

antitrypsin M in ELISA (and with other variants of α1-antitrypsin which for the determination of human serum amyloid P (SAP) which is are homologous with the M-variant for the amino acid sequence). The believed to be intimately linked to β-amyloid in amyloid deposits. The

antibody does not react with Z-variant of α1-antitrypsin. amyloid deposits themselves are involved in the development of the dementia characteristics of Alzheimer disease, hence the opportunity Use: E, WB for further study of the involvement of SAP in this degenerative disease. Quantity: 1 ml This antibody is recommended together with the antibody MON 6006 for use in a capture ELISA.

Use: E Quantity: 250 µg

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Annexin V BromodeoxyUridine/IododeoxyUridine DR6 Fatty Acid Binding Protein, Heart (BrdU/I) Cat.no. MON 9020 (Monoclonal) Cat. no. MON 3078 (Monoclonal) NEW Cat.no. MON 2020 (Monoclonal) Cat.no. MON 8006 (Monoclonal) Clone UMC01 Clone DR-6-04-EC Clone 67D3 The antibody RUU-WAC2A is specific for Annexin V. It shows no cross Clone IU-4 The antibody recognizes human DR6. This monoclonal antibody (subclass IgG1) binds to human heart FABP. reaction with Annexins I, II, III, IV, VI, VII and VIII in ELISA. It exhibits The antibody recognizes both bromodeoxyuridine (BrdU) and iodo- Immunogen: A fusion protein representing amino acids 42-335 It shows no binding to human intestinal or liver FABP. The antibody stains species specificity, it does not recognize monkey and rat Annexin V in deoxyridine (IdU) incorporated into DNA, nucleotides and nucleosides. (extracellular part) of human DR6 linked to the Fc portion of human heart muscle cells and striated skeletal muscle cells in histochemistry. ELISA. Immunogen source: recombinant human Annexin V. IU-4 is recommended where the DNA is lightly substituted and optimum IgG1 was used as an immunogen. antibody affinity is critical. IU-4 is only available unconjugated and Use: E, WB, F, P, IP Use: IP, E, F, P must be used with a second antibody for detection. Use: C, IP Quantity: 100 µg Quantity: 100 µg Quantity: 100 µg Also available as BIOTIN conjugate (MON 2020B - 50 µg) Use: C, P Quantity: 1 ml Beta Glucuronidase Eosinophil Major Basic Protein Fatty Acid Binding Protein, Intestinal C-peptide Cat. no. MON 5059 (Monoclonal) Cat.no. MON 6008 (Monoclonal) Cat.no. PS 022 (Polyclonal)

Clone 105 Cat.no. MON 5021 (Monoclonal) Clone BMK-13 The antibody reacts with human I-FABP of both natural and recombinant This antibody reacts with an non-active centre epitope on human BMK-13 binds to the 10 kD eosinophil Major Basic Protein (MBP) of origin. Intestinal FABP is specific localized in the small bowel epithelium. placental and liver β-Glucuronidase. Does not cross react with rat Clone C-PEP-01 both resting and activated eosinophils in cytospins and frozen sections β-Glucuronidase. The antibody is highly specific for human C-peptide of human pro-insulin. of bronchial and skin biopsies of allergic sites and normal sites and thus Use: F, P, IP, WB, E No cross reactions were found with other serum proteins. can be used as a “pan-eosinophil” marker. The antibody BMK-13 stains Quantity: 100 µg Use: E, WB, F in frozen sections of bronchial biopsies from atopic asthmatics, rhinitics Quantity: 1 ml Use: E, P and normal non-atopic subjects, substantially higher counts of positive Quantity: 100 µg cells when compared to EG1, EG2 and chromotrope 2R. BMK-13 cross Fatty Acid Binding Protein, Liver reacts weakly with human basophils, which also contain low level of Biotin this protein. It does not cross react with any other human protein or cell. Dinitrophenyl hapten Cat.no. MON 6024 (Monoclonal) Use: F, P, E Cat. no. MON 5042 (Monoclonal) Quantity: 1 ml (MON 6008-1) Clone K5A6 Cat.no. MON 5070 (Monoclonal) 5 ml (MON 6008-5) This monoclonal antibody binds to human liver FABP of both natural Clone BIO-8 and recombinant origin. It shows no binding to human intestinal of The antibody recognizes an epitope that is exposed by protein-biotin Clone LO-DNP-2 heart FABP. Liver FABP is localized in the liver and intestinal epithelium. and nucleic acid-biotin conjugates. The antibody is useful in all detection Rat monoclonal antibody against Dinitrophenyl hapten. Immunogen: Fatty Acid Binding Protein, Heart systems with biotinylated probes and antibodies. DNP-ASCARIS, Nippostrongylus brasiliensis-DNP. Avidity: 1.7 x 1010 M-1 Use: F, WB Quantity: 100 µg Use: FS Use: E Cat.no. MON 2019 (Monoclonal) Also available as BIOTIN conjugate (MON 6024B - 50 µg) Quantity: 1 ml Quantity: 100 µg Clone 66E2 This monoclonal antibody (subclass IgG1) binds to human heart FABP. Fatty Acid Binding Protein, Liver BromodeoxyUridine (BrdU) DR4 (TRAIL-R1), Azide Free It shows no binding to human intestinal or liver FABP. The antibody stains heart muscle cells and striated skeletal muscle cells in histochemistry. Cat.no. MON 6023 (Monoclonal) Cat.no. MON 8003 (Monoclonal) Cat. no. MON 3077 (Monoclonal) NEW Use: E, WB, F, IP Quantity: 100 µg Clone L2B10 Clone IIB5 Clone DR-4-02 Also available as BIOTIN conjugate (MON 2019B - 50 µg) This monoclonal antibody binds to human liver FABP of both natural BrdU is a thymidine analogue and when offered to proliferating cells it The antibody recognizes human DR4 (TRAIL-R1). and recombinant origin. It shows cross reactivity with rat L-FABP and is incorporated into reduplicating cells. The antibody is specific for DNA Immunogen: Fusion protein containing the extra-cellular part of TRAIL- no binding to human intestinal or heart FABP. Liver FABP is localized in in which BrdU has been incorporated. Immunogen: BrdU coupled to BSA. R1 and the constant part of the heavy chain of the human IgG1. the liver and intestinal epithelium. In immunoassays this antibody reacts strongly with free or carrier- protein coupled BrdU but not with other nucleosides. In immuncyto- Use: C, IP, FS Use: F, WB chemistry the antibody only recognizes BrdU in denaturated (single Quantity: 100 µg Quantity: 100 µg stranded) DNA. The BrdU antibody is 100% cross reactive with Jodo- Also available as BIOTIN conjugate (MON 6023B - 50 µg) Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies.

Use: IF, C, F, P Quantity: 1 ml

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Fatty Acid Binding Protein, Liver Glucose-dependent Insolinetropic HNP 1-3 (Human Neutrophil Defensins 1-3) Laminin Peptide, Pig Cat.no. PS 021 (Polyclonal) Cat. no. MON 2062 (Monoclonal) Cat.no. PS 040 (Polyclonal) Cat.no. PS 219 (Polyclonal) The antibody reacts with rat L-FABP of both natural and recombinant Clone D21 Antibodies to Laminin localize exclusively in basement membranes. origin. Liver FABP is localized in hepatocytes and brush cells found in Immunogen: Pure porcine GIP. Tested on pig tissues. Positive control: Human neutrophil defensins (α-defensins) belong to the family of Laminin is found throughout the basement membrane, but the reaction the biliary tract. Localization of FABP is demonstrated in mucosa of Frozen sections of pig small intestine. Absorption with 10-100 µg cationic trisulfide-containing microbicidal peptides. Besides microbicidal, with the antibodies to Laminin is more intense in the lamina lucida, small and large intestine and in intestinal metaplasia of the gastric immunogen per ml diluted antiserum abolishes the staining, while the peptides exert chemotactic, immunomodulating and cytotoxic the part of the basement membrane adjacent to the cell membrane. mucosa. Fatty acid binding proteins (FABP) are small (approximately CCK-39, VIP and secretin do not. activity and participate in host defense and inflammation. Azurophilic Laminin binds to type IV collagen, a component exclusively localized 13-14 kDa) intracellular proteins with a high degree of tissue specificity. granules of neutrophils contain Human Neutrophil Peptide (HNP) 1-4 in the basement membrane. Laminin has been shown to play a role in FABPs are a class of cytoplasmic proteins that bind long chain fatty Use: P, F which are highly homologous. The three principal human defensins, cell adhesion and attachment in vivo and in vitro. In immunoblots the acids. They are abundantly present in various cell types and seem to Quantity: 50 µl HNP 1-3, are unique to neutrophils and account for about 99 of the antibodies show characteristic reactivity with Laminin from a 8M urea play an important role in the intracellular utilization of fatty acids. total defensin content of these cells. Defensins HNP 1-3 are absent in extract of amnion basement membrane, with a distinct band of There are at least six distinct types of FABP, each showing a specific other leukocyte subsets, thus representing neutrophil specific cell approximately 200-220 kD. Laminin is a non-collagenous protein of pattern of tissue expression. FABP leaks due to its small size rapidly Glucose regulated Protein 94 marker. Measured amount of defensins is 3-5 mg per million human basement membranes. This glycoprotein has a molecular weight of out of ischemically damaged dying cells leading to a rise in serum neutrophils. Defensins are relatively resistant to proteolysis, low pH about 900 kD. By rotary shadowing it has a cross-like shape, consisting levels. Liver FABP is a sensitive marker for cell damage of liver cells in and boiling. Activation of neutrophils leads to rapid release of HNP. of one long arm (400 kD) and three short arms (200 kD). In immunoblots vitro and vivo. Ischemically damaged tissues are characterized Cat.no. MON 3080 (Monoclonal) HNP can be measured in plasma and other body fluids during infection no reactivity was obtained with human type I, III, IV and V collagen. histological by absence (or low presence) of FABP facilitating recognition and inflammation. In normal plasma very low levels of HNP are present. Immunogen: isolated from EHS-mouse sarcoma. Antigen location: of such areas. Next to this L-FABP is a marker for rapid hepatocyte Clone 9G10 Activation of polymorphonuclear leukocytes (PMN) in plasma as occurs basement membrane. Cross reactivity with most mammals. lysis in vitro (as for example in toxicology assays) and for detection of In Western blot mAb 9G10 binds to a glucose-regulated protein with during clotting of blood leads to a rapid release of HNP. Anti HNP 1-3 liver damage during and after transplantation. an apparent molecular mass of approximately 94 kDa. Source: A Lewis antibody clone D21 recognizes natural HNP 1-3 in biological solutions, Use: F, P rat was immunized with partially purified grp94 from chicken oviduct in tissue sections and leukocyte smears fixed with ethanol, methanol/ Quantity: 0.25 ml Use: IP, WB, E cytosol. Splenocytes were fused with NS-1 cells. acetone or paraformaldehyde, in flow cytometry analysis of human Quantity: 100 µg neutrophils stained by cell permeabilization method, in Western-blotting Use: F, WB reaction and HNP 1-3 ELISA. Furthermore the antibody is capable to Myeloperoxidase (MPO), Mouse Quantity: 100 µg recognize synthetic and recombinant HNP 1-3. Fatty Acid Binding Protein, Liver Use: E, C, F, P, WB Cat. no. MON 2074 (Monoclonal) NEW H Type 2 Quantity: 100 µg Cat.no. PS 023 (Polyclonal) Also available as BIOTIN conjugate (MON 2062B - 50 µg) Clone 8F4 Myeloperoxidase (MPO) is a glycoprotein with an alpha2beta2 The antibody reacts with human L-FABP of both natural and recombinant Cat.no. MON 2035 (Monoclonal) heteromultimer expressed in all cells of the myeloid linage. MPO is origin. Liver FABP is localized in the liver and intestinal epithelium. Ku antigen abundantly present in azurophilic granules of polymorphonuclear Clone 19-OLE neutrophils. It is an important enzyme used during phagocytic lysis of Use: F, P, IP, WB 19-OLE reacts with H type 2 antigen. Source: A BALB/c mouse was engulfed foreign particles which takes part in the defense of the Quantity: 100 µg immunized with mucin isolated from a mucinous colonic adeno- Cat.no. MON 5066 (Monoclonal) organism through production of hypochlorous acid (HOCl), a potent carcinoma. Splenocytes were fused with mouse myeloma SP2/0 cells. oxidant. MPO is rapidly released by activated polymorphonuclear Clone Ku15 neutrophils. Involvement of MPO has been described in numerous FITC Use: F, P, E The antibody reacts with the 86 kDa Ku antigen. This antibody is useful diseases such as atherosclerosis, lung cancer, Alzheimer’s disease and Quantity: 100 µg for the study of protein kinase and their interaction with oncoproteins. multiple sclerosis. Autoimmune antibodies to MPO are involved in The antibody recognizes an epitope within amino acid 490 to 707 of Wegeners disease. Since the discovery of MPO deficiency, initially Cat.no. MON 7070 (Monoclonal) the Ku antigen. The antigen isolated for immunization was the A 431 regarded as rare and restricted to patients suffering from severe human epidermoid carcinoma cell line. Cross reactive with human and infections, MPO has more attracted clinical attention. In experimental Clone F4/1 monkey cell nuclei. studies antibodies to MPO can be used for various purposes ranging F4/1 is specific for FITC. Source: F4/1 is a hybridoma formed by fusing from flow cytometric analysis to detection of polymorphonuclear SP-2/0 Ag14 cells with spleen cells of a BALB/c mouse that had been Use: F, P, WB, IP neutrophils in tissue sections. Monoclonal antibody 8F4 also reacts immunized with FITC-KLH. It was selected for anti-fluorescein specificity Quantity: 100 µg with rat MPO. using another fluorescein conjugate. Use: E, C, F Use: E, WB Quantity: 100 µg Quantity: 100 µg

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NP-5 (Neutrophil Defensin 5), Rabbit Peptide YY Phosphotyrosine Secretory Leukocyte Protease Inhibitor (SLPI)

Cat.no. MON 3046 (Monoclonal) Cat.no. PS 229 (Polyclonal) Cat.no. MON 2055 (Monoclonal) Cat.no. MON 2005 (Monoclonal)

Clone R3 Immunogen: Synthetic porcine peptide YY(PYY). Tested on human and Clone P-TYR-01 Clone 31 Alpha defensins belong together with the β-defensins to the family of rat tissues. Positive control: Bouin-fixed paraffin sections of rat colon. This antibody reacts with phosphotyrosine. The antibody exhibits similar The antibody reacts specifically with secretory leukocyte proteinase cationic trisulfide-containing microbicidal peptides. They exert their Frozen sections of rat colon. Absorption with 10-100 µg PYY per ml reactivity to the commercial standard antibody PY20. inhibitor (SLPI; also known as antileukoprotease (ALP)). SLPI is a 11.7 kDa microbicidal activity by permeating the membrane involving the diluted antiserum abolishes the staining, while NPY and PP do not. cationic inhibitor of neutrophil elastase and to a lesser extent of formation of voltage regulated ion channels. Rabbit neutrophils contain Use: WB cathepsin G. It is locally produced by epithelial cells in the lung, skin large amount of alpha defensins (Neutrophil Defensin) NP 1-6. Defensins Use: P, F Quantity: 100 µg and other organs and by Polymorphonuclear leukocytes (PMN) and (in constitute more than 15% of total protein in rabbit neutrophils and Quantity: 50 µl mice) by macrophages. In addition to its proteinase inhibitory properties are accumulated in dense cytoplasmic granules. Rabbit neutrophils that may serve to protect against proteolytic injury, it was recently express all six rabbit alpha-defensins, NP 1 - 6. Among myeloid rabbit Prepro-NPY 68-97 (C-PON) shown that SLPI also displays several other functions such as alpha-defensins NP 1 - 6, NP 3 - 6 are neutrophil specific, while NP 1 - 2 Peptide YY, Mouse antimicrobial and anti-inflammatory activities. These appear to be are expressed in rabbit alveolar macrophages. Defensins are relatively independent of its ability to inhibit PMN serine proteinases. SLPI has resistant to proteolysis, low pH and boiling. Rabbit neutrophil defensins, Cat.no. PS 230 (Polyclonal) also been demonstrated to display antibacterial and antifungal activity whose three-dimension structures are very similar to those of human Cat.no. PS 253 (Polyclonal) at concentrations in which SLPI is present in mucosal secretions including alpha defensins may be helpful in understanding the role of antimicrobial Immunogen: Synthetic Prepro-NPY 68-97 (C-PON). Tested on human those of the lung. Another possible role for SLPI is inhibition of peptides in innate and acquired immunity and in the studies on the Immunogen: Synthetic porcine peptide YY. Tested on mouse, rat and and rat tissues. Positive control: Stefanini-fixed frozen sections of rat protein-disulphide isomerase that is considered essential for invasion role of neutrophils in pathological situations and in various rabbit models cat tissues. Positive control: Frozen sections of rat intestine. Absorption small intestine. Absorption with 10-100 µg immunogen per ml diluted of a cell by the Human Immunodeficiency Virus (HIV). of human diseases. Anti NP 5 antibody clone R3 recognizes natural with 10-100 µg PYY per ml diluted antiserum abolishes the staining. antiserum abolishes the staining. NP-5 in biological solutions, in tissue sections and leukocyte smears Use: F, P, E, IP fixed with ethanol, methanol/acetone or paraformaldehyde, in flow Use: P, F Use: F Quantity: 100 µg cytometry analysis of rabbit neutrophils stained by cell permeabilization Quantity: 50 µl Quantity: 50 µl Also available as BIOTIN conjugate (MON 2005B - 50 µg) method and in Western-blotting reaction. Furthermore the antibody is capable to recognize synthetic and recombinant NP 5. Phenylethanolamine N-Methyltransferase Retinol Binding Protein (RBP) Secretory Leukocyte Protease Inhibitor (SLPI) Use: C, F, P Quantity: 100 µg (PNMT) Cat.no. MON 7071 (Monoclonal) Cat.no. PS 024 (Polyclonal) Cat.no. PS 256 (Polyclonal) Penicillin Clone G4E4 The antibody reacts with secretory leukocyte proteinase inhibitor (SLPI; Immunogen: Phenylethanolamine-N-methyltransferase, from bovine G4E4 reacts with a defined epitope of circulatory RBP from human, also known as antileukoprotease (ALP). SLPI is a 11.7 kDa cationic adrenal medulla. Tested on rat tissues. Positive control: DEPC-fixed monkey, goat, rat and mouse (other species not tested). Source: A BALB/ inhibitor of neutrophil elastase and to a lesser extent of cathepsin G. Cat.no. MON 4040 (Monoclonal) paraffin sections of rat adrenal gland. Absorption with 10-100 µg PNMT c mouse was immunized with human retinol binding protein (RBP) It is locally produced by epithelial cells in the lung, skin and other organs, per ml diluted antiserum abolishes the staining. purified from plasma. Splenocytes were fused with mouse myeloma by Polymorphonuclear leukocytes (PMN) and (in mice) by macrophages. Clone Pen 9 SP2/0 cells. In addition to its proteinase inhibitory properties that may serve to Pen 9 reacts mainly with the thiazolidin ring of penicillin, but not with Use: P, F protect against proteolytic injury, it was recently shown that SLPI also the lactam ring. The nature of the side chain in the penicilloyl group Quantity: 50 µl Use: F, P, E displays several other functions such as antimicrobial and anti- does not effect antibody binding as was shown by testing Pen 9 against Quantity: 100 µg inflammatory activities. These appear to be independent of its ability benzylpenicillin, ampicillin, amoxillin and 6-aminopenicillanic acid. The to inhibit PMN serine proteinases. SLPI has also been demonstrated to presence of carrier protein is not essential for the presentation of the Phosphotyrosine display antibacterial and antifungal activity at concentrations in which antigen associated with Pen 9. Source: Pen 9 was raised in a BALB/c Secretory Component SLPI is present in mucosal secretions including those of the lung. mouse against the benzylpenicilloyl group of penicillium, using a Another possible role for SLPI is inhibition of protein-disulphide penicilloyl-protein (transferrin) conjugate. Splenocytes were fused with Cat.no. MON 2054 (Monoclonal) isomerase that is considered essential for invasion of a cell by the X63-Ag8.653 cells. Cat.no. MON 3021 (Monoclonal) Human Immunodeficiency Virus (HIV). Clone P9V6 Use: E This highly specific monoclonal antibody reacts with phosphotyrosine. Clone SC-05 Use: WB, E Quantity: 100 µg It does not cross react with phosphoserine or phosphothreonine. The antibody reacts with the human 80 kDa secretory component Quantity: 100 µg glycoprotein (both free and bound in secretory IgA). Cross reactivity Use: WB, E with non-human species were not tested. Quantity: 100 µg Also available as BIOTIN conjugate (MON 2054B - 50 µg) Use: F, WB, P, IP Quantity: 100 µg

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Streptavidin Alpha-Fetoprotein (AFP) Alpha-Fetoprotein (AFP)

Cat. no. MON 5043 (Monoclonal) Cat.no. MON 5032A (Monoclonal) Cat.no. MON 4036 (Monoclonal)

Clone STREP-10 Clone AFP-01 Clone C3 The antibody is specific for streptavidin. It lacks cross reactivity with The antibody is highly specific for human alpha-fetoprotein. No cross C3 has been characterized in the ISOBM TD-2 workshop and assigned avidin. The antibody does not interfere with biotin binding. reactions were found with other serum proteins. The epitope is dependent by K. Nustad to a group of mAb with low affinity binding to human on Ca2+ (sensitive to EDTA). alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of Use: F, P, E 70 kD. Its gene has been sequenced and its structure identified. AFP is Quantity: 1 ml Use: E expressed in fetal liver and is normally absent in healthy adult tissues. Quantity: 100 µg It is expressed in all yolk sac tumours, in some germ cell tumours and in hepatocellular carcinomas. Source: A BALB/c mouse has been Tenascin, 210 kD, 300 kD immunized with alpha fetoprotein isolated from serum of hepatoma Alpha-Fetoprotein (AFP) patients. Splenocytes were fused with mouse myeloma cell line P3-X63-Ag8.653. Cat.no. MON 7025 (Monoclonal) Cat.no. MON 5032B (Monoclonal) Use: F, P, E, WB Clone T2H5 Quantity: 100 µg This monoclonal reacts with human tenascin, an extra cellular matrix Clone AFP-11 glycoprotein. During embryonic development Tenascin appears to The antibody is highly specific for human alpha-fetoprotein. No cross serve a regulating role in epithelial diffentiation. In many mature reactions were found with other serum proteins. Alpha-Fetoprotein (AFP) tissue Tenascin disappears, but it reappears in tissue regeneration and repair and in neoplasia. Tenascin expression has been noted in a Use: E, P variety of epithelial neoplasms, including skin-, prostate-, breast- and Quantity: 500 µg Cat.no. MON 4037 (Monoclonal) colon cancer. The antibody is known to react with at least two forms of Tenascin one of approximately 210 kDa and one of approximately Clone D10 300 kDa. Antigen origin: isolated from mammary tumour cells. Antigen Alpha-Fetoprotein (AFP) D10 has been characterized in the ISOBM TD-2 workshop and assigned location: extra cellular matrix. by K. Nustad to group D of a cluster of 6 major epitopes of human alpha fetoprotein. Human alpha fetoprotein is an oncofetal protein of Use: F Cat.no. MON 4035 (Monoclonal) 70 kD. Its gene has been cloned and sequenced and its structure Quantity: 1 ml identified. AFP is expressed in fetal liver and is normally absent in Clone C2 healthy adult tissues. It is expressed in all yolk sac tumours, in some C2 is characterized in the ISOBM TD-2 workshop and assigned by germ cell tumours and in hepatocellular carcinomas. Source: A BALB/c Transferrin K. Nustad to group E of a cluster of 6 major epitopes of human alpha mouse has been immunized with alpha fetoprotein isolated from serum fetoprotein. Human alpha fetoprotein is an oncofetal protein of 70 kD. of hepatoma patients. Splenocytes were fused with mouse myeloma Its gene is cloned and sequenced and its structure identified. AFP is cell line P3-X63-Ag8.653. Cat.no. MON 5016 (Monoclonal) expressed in fetal liver and is normally absent in healthy adult tissues. It is expressed in all yolk sac tumours, in some germ cell tumours and Use: F, P, E, WB Clone HTF-14 in hepatocellular carcinomas. Source: A BALB/c mouse has been Quantity: 100 µg The antibody is highly specific for human transferrin. The antibody immunized with alpha fetoprotein isolated from serum of hepatoma recognizes the epitope located on N-terminal domain of human patients. Splenocytes were fused with mouse myeloma cell line transferrin. Cross reactions with porcine rabbit and rat transferrin are P3-X63-Ag8.653. Alpha-Fetoprotein (AFP) observed. The antibody blocks the binding of transferrin to its receptor. No cross reactions were found with other serum proteins. Use: F, P, E, WB Quantity: 100 µg Cat.no. MON 4034 (Monoclonal) Use: E, WB, B, IP Quantity: 100 µg Clone MBS-12 MBS-12 specifically detects AFP. This protein is one of the major serum proteins in the early life of mammals. AFP is an oncodevelopmental gene product which is expressed at high levels in the embryonic yolk sac and fetal liver. AFP production is reactivated in the adult during liver regeneration and hepatocarcinogenesis. Source: A BALB/c mouse was immunized with alpha-fetoprotein (AFP) and the isolated splenocytes were fused with NS1-cells.

Use: F, E Quantity: 100 µg

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Alpha-Fetoprotein (AFP) Apolipoprotein AI, apoAI Apolipoprotein AI, apoAI C3d receptor, CR2, CD21

Cat.no. PS 076 (Polyclonal) Cat.no. PS 072 (Polyclonal) Cat.no. PS 096 (Polyclonal) Cat.no. MON 7019 (Monoclonal)

This antibody is directed against human alpha-Fetoprotein established Apolipoprotein AI (apoAI), a 28 kDa plasma protein and component of Apolipoprotein AI (apoAI), a 28 kDa plasma protein and component of Clone 4C5 in numerously immunized rabbits. The antiserum was subjected to HDL. The antigen is isolated from human plasma and used at a purity HDL. The antigen is isolated from human plasma and used at a purity This antibody reacts with the CD21 (140kD) molecule, expressed absorption with immobilized human albumin fraction and globulin of 95% as antigen for the immunization of rabbits. Pooled antisera are of 95% as antigen for the immunization of sheep. Pooled antisera are (moderate) on mature B-cells and (at high density) on follicular dendritic fractions and fibrinogen in order to remove non-specific antibodies. The passed over DEAE-cellulose to produce IgG-enriched fraction, which is passed over DEAE-cellulose to produce IgG-enriched fraction, which is cells (FDC). affinity purified antibody PS076 is obtained by binding to immobilized further subjected to absorption with immobilized human albumin and further subjected to absorption with immobilized human albumin and human alpha-Fetoprotein (antigen used for immunization), followed immmunoglobuline fractions in order to remove non-specific antibodies. immmunoglobuline fractions in order to remove non-specific antibodies. Use: C, F, IF by elution with acidic buffer, titration, dialysis, dispensing and The affinity purified antibody PS072 is obtained by binding to The affinity purified antibody PS096 is obtained by binding to Quantity: 1 ml lyophilization. Specificity of the antisera is confirmed by ELISA (OPD) immobilized human apolipoprotein AI (antigen used for immunization), immobilized human apolipoprotein AI (antigen used for immunization), on human alpha-Fetoprot-ein versus albumin, IgG, IgM. Tests with followed by elution with acidic buffer, neutralisation, dialysis, followed by elution with acidic buffer, neutralisation, dialysis, immunoblotting of fetal and adult sera. Ouchterlony double diffusion, dispensing and lyophilized. Specificity of the antisera is confirmed by dispensing and lyophilized. Specificity of the antisera is confirmed by CA-19.9 immunoelectrophoresis. No cross reactivity with human plasma proteins. direct ELISA with purified human apoAI adsorbed to plastic wells, by direct ELISA with purified human apoAI adsorbed to plastic wells, by Ouchterlony immunodiffusion, and by dot-blot assay with different Ouchterlony immunodiffusion, and by dot-blot assay with different Use: WB, E, A fractions of lipoproteins. No interference is caused by delipidated human fractions of lipoproteins. No interference is caused by delipidated human Cat.no. MON 3060 (Monoclonal) Quantity: 1 mg plasma absorbed by immobilized monoclonal antibody to apoAI plasma absorbed by immobilized monoclonal antibody to apoAI (MON 5030). (MON 5030). Clone 121SLE The specificity of the antibody was confirmed on Workshop TD6. Alpha-Fetoprotein (AFP) Use: F, E Use: E, F Sialyl-Lewis a containing glycolipids. Quantity: 500 µg Quantity: 500 µg Use: C, E, P Cat.no. PS 077 (Polyclonal) Quantity: 100 µg Apolipoprotein AI, apoAI Apolipoprotein E This antibody is directed against human alpha-Fetoprotein established in numerously immunized goats. The antiserum was subjected to Carcinoembryonic antigen, CEA absorption with immobilized human albumin fraction and globulin Cat.no. PS 095 (Polyclonal) Cat.no. PS 070 (Polyclonal) fractions and fibrinogen in order to remove non-specific antibodies. The affinity purified antibody PS077 is obtained by binding to immobilized Apolipoprotein AI (apoAI), a 28 kDa plasma protein and component of Apolipoprotein E (apoE), a 34 kDa plasma protein and component of Cat.no. MON 9008 (Monoclonal) human alpha-Fetoprotein (antigen used for immunization), followed HDL. The antigen is isolated from human plasma and used at a purity LDL. The antigen is isolated from human plasma and used at a purity by elution with acidic buffer, titration, dialysis, dispensing and of 95% as antigen for the immunization of sheep. Pooled antisera are of 95% as antigen for the immunization of rabbits. Pooled antisera are Clone PARLAM4 lyophilization. Specificity of the antisera is confirmed by ELISA (OPD) passed over DEAE-cellulose to produce IgG-enriched fraction, which is passed over DEAE-cellulose to produce IgG-enriched fraction, which is This monoclonal antibody is reactive with human carcinoembryonic on human alpha-Fetoprot-ein versus albumin, IgG, IgM. Tests with further subjected to absorption with immobilized human albumin and further subjected to absorption with immobilized human albumin and antigen (CEA), a tumour associated antigen with oncofetal characteristics. immunoblotting of fetal and adult sera. Ouchterlony double diffusion, immmunoglobuline fractions in order to remove non-specific immmunoglobuline fractions in order to remove non-specific Immunoblotting - antibody reacts with a single band of 180 kD. In immunoelectrophoresis. No cross reactivity with human plasma proteins. antibodies. Specificity of the antisera is confirmed by direct ELISA with antibodies. Specificity of the antisera is confirmed by direct ELISA with immunohistochemistry on tissue sections: no reactivity with granulo- purified human apoAI adsorbed to plastic wells, by Ouchterlony purified human apoE adsorbed to plastic wells, by Ouchterlony cytes, bile canaliculi, alveolar epithelium. No cross reactivities have Use: WB, E, A immunodiffusion, and by dot-blot assay with different fractions of immunodiffusion, and by dot-blot assay with different fractions of been observed. CEA can be found in a large variety of carcinomas, but Quantity: 1 mg lipoproteins. No interference is caused by delipidated human plasma lipoproteins. No interference is caused by delipidated human plasma also in the tissue of non-neoplastic diseases and normal epithelia. absorbed by immobilized monoclonal antibody to apoAI (MON 5030). absorbed by immobilized monoclonal antibody to apoE (MON 5029). Despite this lack of specificity, immunohistochemical detection of CEA is frequently used for the histopathologial diagnosis of human tumours. Apolipoprotein AI, apoAI Use: E, F, P Use: F, E Most polyclonal, non purified, CEA antisera show cross reactivity with Quantity: 1 ml Quantity: 1 ml related antigens such as biliary glycoprotein (BGP) and non-specific cross reacting antigen 1/11 (NCA). Our monoclonal CEA antibody does Cat.no. PS 071 (Polyclonal) not show cross reactivity neither with BGP nor with NCA. Antigen isolated Beta-2-Microglobulin from: membrane of human colon carcinoma cells. Antigen location: Apolipoprotein AI (apoAI), a 28 kDa plasma protein and component of membrane, cytoplasm. Cross reactivity: reacts only with human CEA. HDL. The antigen is isolated from human plasma and used at a purity of 95% as antigen for the immunization of rabbits. Pooled antisera are Cat.no. MON 1031A (Monoclonal) Use: F, P passed over DEAE-cellulose to produce IgG-enriched fraction, which is Quantity: 1 ml further subjected to absorption with immobilized human albumin and Clone B2M-01 immmunoglobuline fractions in order to remove non-specific The antibody reacts with beta2-microglobulin associated with cell- antibodies. Specificity of the antisera is confirmed by direct ELISA with surface MHC Class 1 molecules and other membrane antigens as well purified human apoAI adsorbed to plastic wells, by Ouchterlony as with soluble forms of beta2-microglobulin. No species cross reactivity immunodiffusion, and by dot-blot assay with different fractions of was found with lymphocytes of pig, cow, dog, chicken, rabbit and with lipoproteins. No interference is caused by delipidated human plasma thymocytes of mouse. Weakly positive were PBL of the monkey absorbed by immobilized monoclonal antibody to apoAI (MON 5030). cercopithecus aethiops.

Use: F, E Use: C, IP, WB, E Quantity: 1 ml Quantity: 100 µg

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Carcinoembryonic antigen, CEA Chromogranin A + B Determinant discriminating between nevo Endothelial differentiation marker cellular nevi and malignant Melanomas Cat.no. PS 261 (Polyclonal) Cat.no. PS 058 (Polyclonal) Cat.no. MON 6013 (Monoclonal) Cat.no. MON 7001 (Monoclonal) Immunogen: Human CEA. Tested on human tissues. Positive control: Chromogranins are acidic glycoproteins co-stored and co-released with Clone 1F10 Bouin-fixed paraffin sections of human colon carcinoma. Absorption with peptides and amines in a variety of neuroendocrine tissues, both normal Clone PAL-M1 The antibody reacts with fully differentiated continuous human 10-100 µg immunogen per ml diluted antiserum abolishes the staining. and neoplastic. This polyclonal antibody recognizes chromogranin A This antibody stains the majority of primary melanomas and the large endothelial cells of blood vessels. It shows no side reactions with and B. This dual specificity makes it a potent marker of peptide majority of metastases. Local staining of dysplastic nevocellular naevi non-endothelial cells. The antigen is inconsistently present on or absent Use: P, F hormone-producing endocrine cells and tumours. Specific reactive with may be observed. Common nevocellular naevi rarely stain. on discontinuous sinusoidal endothelial cells and lymphatic endothelial Quantity: 50 µl pituitary antrum, duodenum, small intestines and pancreas when used cells. In in vitro cultures of human umbilical vein endothelial cells the in immunohistochemistry. Antigen origin: isolated from human Use: F antigen rapidly disappears from the cells. The expression of the antigen Pheochromocytoma. Antigen location: cytoplasm. Quantity: 1 ml (MON 7001-1) was found to be dependent on the culture conditions, positive 0-70% Carcinoma-associated antigen MAM-6 5 ml (MON 7001-5) HUVEC. Frequent renewal of the medium containing foetal calf serum Use: F, P leads to enhanced expression. Quantity: 0.25 ml Cat. no. MON 9005 (Monoclonal) Determinant discriminating between nevo Use: F, IF Quantity: 0.5 ml cellular nevi and malignant Melanomas Clone 115D8 Chromogranin A+B This antibody recognizes MAM-6, a mucus glycoprotein of > 400 kD that is located mainly in the glycocalyx of most glandular epithelial cells Cat.no. MON 7002 (Monoclonal) Endothelium and in the cytoplasm and on the membrane of most carcinoma cells. Cat.no. PS 262 (Polyclonal) The antibody reacts very strongly with most carcinomas. It occasionally Clone PAL-M2 shows a very weak cross reactivity with sarcomas and leukaemia’s. It Immunogen: Chromogranin A+B purified from human pheochromo- This antibody stains a minority of primary melanomas and half of the Cat.no. MON 6002 (Monoclonal) does not react with normal intestine or colorectal adenomas, but reacts cytoma. Tested on human tissues. metastatic lesions tested. It rarely stains dysplastic naevi or common with colorectal carcinomas. Positive control: Bouin-fixed paraffin sections of human pancreas. cellular naevi using standard immunohistochemical conditions. The Clone EN4 Absorption with 10-100 µg Chromogranin A+B per ml diluted antiserum antibody recognizes two protein bands in immunoblotting with a This antibody stains all human blood vessels including brain micro Use: F, P, IP, WB abolishes the staining. molecular weight of 95-100 kD. vessels. Both large and small vessels are equally reactive. The EN4 Quantity: 1 ml (MON 9005-1) antigen is preserved in endothelial cells in culture unlike the PAL-E 5 ml (MON 9005-5) Use: P, F Use: F antigen which is lost. It stains strongly mouse fibroblasts transfected Quantity: 50 µl Quantity: 1 ml (MON 7002-1) with the human CD31 gene. On surface-iodinated Jurkat T-cells it 5 ml (MON 7002-5) recognizes the 130 kD CD31 antigen. EN4 also binds to guinea-pig Chromogranin A and cat endothelium, but not to rabbit, bovine, sheep, dog, rat or mice Colon carcinoma endothelial cells. No other structures in the skin, heart, kidney, tonsils Endothelial internalizing surface antigen or spleen are stained with this antibody. Cat.no. MON 9014 (Monoclonal) Cat.no. MON 7007 (Monoclonal) Use: F, C Clone LK2H10 Cat.no. MON 6009 (Monoclonal) Quantity: 1 ml The antibody is specific for Chromogranin A. Specificity was ascertained Clone CC-1 by immunoblotting and immunohistochemistry. The antibody This antibody is a marker for colon carcinoma’s. Clone GES-HB predominantly reacts with a 68 kDa protein. Antigen was isolated from The antibody reacts with a protease sensitive surface antigen that is Endothelium human Pheochromocytoma. Antigen location, cytoplasm. Cross reactivity Use: F present on human endothelial cells of various origins: microvasculature, with monkey and pig. Quantity: 1 ml aorta and arterial and venous umbilical vessels. Cross reactions with blood cells or plasma proteins were not found. The antibody-antigen Cat.no. MON 6001 (Monoclonal) Use: F, P, WB complex is rapidly internalized by metabolically active endothelial cells Quantity: 1 ml Colon carcinoma (at 37°C within 15-30 min.). Also liposomes coated with the antibody Clone PAL-E are rapidly internalized by endothelial cells. The internalization does The antibody only stains human and various animal (goat, rabbit, pig, not affect the growth and morphological appearance of endothelial dog) blood vessels with exception of arteries. It does not stain rat, Cat.no. MON 7016 (Monoclonal) cells in culture. The internalization of the antibody is not dependent on mouse and chicken endothelium. the Fc part of the molecule. Specific technical details will dependent Clone CC-3 on the methods to be used. Use: F This antibody is a marker for colon carcinoma’s. Quantity: 1 ml (MON 6001-1) Use: F, C 5 ml (MON 6001-5) Use: IP, F Quantity: 200 µg Quantity: 1 ml

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GCDFP-15, SABP, GP-17 High Density Lipoprotein, HDL 2, HDL 3, HSP-60, heat-shock protein Low Density Lipoprotein, APO-B APO-A1 Cat.no. MON 7027 (Monoclonal) Cat.no. MON 5063 (Monoclonal) Cat.no. MON 5027 (Monoclonal) Cat.no. MON 5030 (Monoclonal) Clone 5E7 Clone LK1 Clone 4C11 This monoclonal antibody is elicited against the salivary glycoprotein Clone 1C5 Heat-shock proteins have been shown to be critical antigens in a number The antibody reacts with low density lipoproteins of human plasma. ‘Human Extra Parotid Glycoprotein’ EP-GP. This protein is identical to The antibody reacts with high density lipoproteins (HDL 2, HDL 3, of autoimmune diseases. In human arthritis and in experimentally It is specific for fragment T3, the middle thrombolytic peptide of human the gross cystic disease fluid protein GCDFP-15, secretory actin binding apo-A1) of human plasma. No cross reactions with other human induced arthritis in animals, diseased development was seen to apo-B. No cross reactions with other human plasma proteins were protein (SABP) and glycoprotein 17 (GP-17). In immunoblotting, the plasma proteins were detected. coincide with development of immune reactivity directed against not detected. antibody reacts with its antigen in saliva, tear fluid, seminal plasma only bacterial HSP60, but also against its mammalian homologue. The and sweat. It reacts in immunohistological sections of submandibular Use: E, F LK1 monoclonal antibody reacts with mammalian 60kD heat-shock Use: E, F tissues. GCDFP-15 is expressed by breast carcinomas (primary and Quantity: 500 µg protein (HSP-60 family of heat-shock proteins), and is not cross reactive Quantity: 1 ml metastatic lesions) with apocrine features. with prokaryotic (bacterial or parasitic) homologues.

Use: E, WB, F HSP-27, heat-shock protein Use: F, E, WB Low Density Lipoprotein, APO-B Quantity: 1 ml Quantity: 1 ml

Cat.no. MON 5091 (Monoclonal) Cat.no. MON 5028 (Monoclonal) F VIII related antigen, Endothelial cell marker Low Density Lipoprotein, APO-B Clone G3.1 Clone 2E3 G3.1 reacts specifically with HSP27 in human tissues and cell lines The antibody reacts with low density lipoproteins of human plasma. Cat.no. MON 6014 (Monoclonal) such as MCF-7. HSP27, also referred to as the Estrogen-Regulated 24K Cat.no. MON 5025 (Monoclonal) It is specific for fragment T2, the carboxyterminal thrombolytic peptide protein and HSP28, is one of several small heat shock proteins (HSP) of human apo-B. No cross reactions with other human plasma proteins Clone KG 7/30 produced by all organisms studied. HSP27 synthesis is induced by Clone 5F8 were detected. The antibody reacts with F VIII:R a cytoplasmic antigen that is present elevated temperature, as well as estrogen in hormone responsive cells. The antibody reacts with low density lipoproteins of human plasma. in the endothelial cells of all organs. Furthermore the antigen is present Interestingly, human HSP27 also shares greater than 50% homology It is specific for fragment T4, the amino terminal thrombolytic peptide Use: E, F in thrombocytes and megakaryocytes. The antigen expression is minimal with low molecular weight Drosophila HSP’s and mammalian alpha- of human apo-B. No cross reactions with other human plasma proteins Quantity: 1 ml in capillaries of lung and kidney, whereas in tumours a variable crystelline lens protein. Because of the estrogen responsive nature of were detected. It reacts not only with B100 but also with B48, this expression can be observed. In in vitro cultures of endothelial cells the HSP27, this protein has been studied extensively in human estrogen makes it a pan-antibody for APO-B-containing particals in plasma. antigen expression decreases during prolonged culture. responsive tissues such as cervix, endometrium and breast tissue. (very) Low Density Lipoprotein, APO-E Therefore HSP27 may be useful in classifying various hormone Use: E, F Use: F, IF sensitive tumours. Source: A BALB/c mouse was immunized with Quantity: 1 ml Quantity: 0.5 ml partially purified HSP27 derived from MCF-7 cytosol. Splenocytes were Cat.no. MON 5029 (Monoclonal) fused with NSI-1.Ag4.1 cells. Low Density Lipoprotein, APO-B Clone 3D12 Hepatocytes Use: F, P, E, IP, WB The antibody reacts with very low density lipoproteins (VLDL) of human Quantity: 100 µg plasma. It is specific for human apo-E. No cross reactions with other Cat.no. MON 5026 (Monoclonal) human plasma proteins were detected. This antibody reacts as well Clone EP-1 Cat.no. MON 2058 (Monoclonal) with LDL as with VLDL. This antibody recognizes all alleles of APO-E. Clone EP-2 Cat.no. MON 2059 (Monoclonal) HSP-60, heat-shock protein Clone 12G10 The antibody reacts with low density lipoproteins of human plasma. Use: E, F, P Monoclonal antibodies stains human liver canaliculi and a subset of It is specific for fragment T4, the amino terminal thrombolytic peptide Quantity: 1 ml hepatocellular carcinomas. In frozen sections, it stains liver canaliculi Cat.no. MON 5062 (Monoclonal) of human apo-B. No cross reactions with other human plasma proteins strongly and may be used as a marker of this hepatic substructure. Cell were detected. preparations of hepatocellular carcinoma biopsies and cell lines are Clone LK2 Leukaemia cell line HL-60 found to bind on the cell surface. Monoclonal antibodies stains liver Heat-shock proteins have been shown to be critical antigens in a number Use: E, F canaliculi in normal liver sections and is positive on the cell surface of of autoimmune diseases. In human arthritis and in experimentally Quantity: 1 ml human liver carcinomas. Monoclonal antibodies to liver cell processes induced arthritis in animals, disease development was seen to coincide Cat.no. MON 8035 (Monoclonal) are useful in the identification of hepatic carcinomas and normal with development of immune reactivity directed against not only organ structures. Two monoclonal antibodies to different liver bacterial HSP60, but also against its mammalian homologue. Antibody Clone IPO-M6 substructures are offered which bind to subsets of hepatic carcinomas. LK-2 recognizes both mammalian and bacterial HSP60. The epitope IPO-M6 reacts with human leukaemia cell line HL-60 and immuno- which the LK2 recognizes is located between amino acids 383-44 of precipitates with two proteins with MW of 48 and 52 kDa. IPO-M6 Use: F, P human HSP60. LK-2 showed a raised level of staining in light does not stain B-cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell Quantity: 100 µg microscopy immunohistochemistry of synovial membranes in patients lines CCRF-HSB2, Jurkat and Molt-4. Source: IPO-M6 was generated with juvenile chronic arthritis. after immunization of a BALB/c mouse with human cell line HL-60.Mouse splenocytes were fused with the mouse myeloma cell line Use: F, E, WB P3-X63-Ag8.653. Quantity: 1 ml Use: F, C Quantity: 100 µg

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Melanoma-associated antigen Melanoma-associated antigen MUC1 (polymorphic epithelial mucin) MUC1 (polymorphic epithelial mucin)

Cat.no. MON 7003 (Monoclonal) Cat.no. MON 7004 (Monoclonal) Cat.no. MON 6040 (Monoclonal) Cat.no. MON 6042 (Monoclonal)

Clone NK-I/C3 Clone 126E7 Clone S1D12D7 This antibody recognizes a heterogeneous 25-110 kD glycoprotein that Clone NKI-M6 126E7 reacts with MUC1, a large transmembrane glycoprotein expressed S1D12D7 reacts with MUC1, a large transmembrane glycoprotein is located mainly in the inner side of membranes of cytoplasmic This antibody recognizes a high molecular weight proteoglycan with a on the ductal surface of normal glandular epithelia. The extra-cellular expressed on the ductal surface of normal glandular epithelia. The vesicles in melanoma cells. The antigen has a 25 kD unglycosylated molecular weight of > 450 kD (chondroitin sulfate) and 250 kD domain of MUC1 largely consists of a highly conserved, O-glycosylated extra cellular domain of MUC1 largely consists of a highly conserved, precursor in formalin fixed and paraffin-embedded tissue sections. (core protein). The antibody reacts strongly with melanoma cells derived 20 amino acids tandem repeat which can occur 30-100 times per O-glycosylated 20 amino acids tandem repeat which can occur 30-100 Cross reactivity: The antibody reacts with some mucus producing from cell lines and short term cultures and reacts preferentially with molecule depending on the length of the allele involved. In the vast times per molecule depending on the length of the allele involved. In tumours, carcinoids, carcinomas of the thyroid, mast cells, histiocytes melanoma cells in frozen tissue sections (1). The antibody can also be majority of human carcinomas this protein is up-regulated and poorly the vast majority of human carcinomas this protein is up-regulated in tumour regions and with cells with secretory functions such as used to detect melanoma lesions in vivo (2). Cross reactivity: The glycosylated and appears on the cell surface in a non-polarized fashion. and poorly glycosylated and appears on the cell surface in a non- salivary glands, bronchial glands, sweat glands, pancreas and prostate. antibody reacts with most naevi and perineurium, and shows weak The dominant epitope of 126E7 has not yet been established with polarized fashion. The dominant epitope of S1D12D7 is “GVTSAP” as reactivity with hair follicles. “epitope fingerprinting”. Source: A Balb/c mouse was immunized with established with “epitope fingerprinting”. Source: A Balb/c mouse was Use: P a Breast Cancer crude membrane preparation. Splenocytes were fused immunized with a MUC1 60mer tandem repeat

Quantity: 1 ml (MON 7003-1) Use: F with SP2/0 mouse myeloma cells. Stability of the hybridomas was NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH conjugated to bovin serum 5 ml (MON 7003-5) Quantity: 1 ml (MON 7004-1) established by sub cloning four times. albumin. Splenocytes were fused with SP2/0 mouse myeloma cells. 5 ml (MON 7004-5) Stability of the hybridomas was established by sub cloning four times. Use: F, P, E Melanoma-associated antigen Quantity: 100 µg Use: F, P, E Melanoma marker Quantity: 100 µg Cat.no. MON 7006 (Monoclonal) MUC1 (polymorphic epithelial mucin) Cat.no. MON 7017 (Monoclonal) MUC1 (polymorphic epithelial mucin) Clone NKI/beteb This antibody recognizes a (pre)melanosomal 100 + 7 kd antigen Clone 2g12 Cat.no. MON 6041 (Monoclonal) (glycoprotein). The antibody reacts with melanomas, clear cells sarcomas This antibody will react with the 240-270 kDa membrane antigen and Cat.no. MON 6043 (Monoclonal) (melanoma of soft tissue), nevocellular nevi, and normal melanocytes. is an important marker for melanoma studies. Clone 139H2 Except for one case of non-Hodgkin’s lymphoma in which macrophages 139H2 reacts with MUC1, a large transmembrane glycoprotein expressed Clone VU-3C6 were positive, no reactions with other tumours or tissues have been Use: WB on the ductal surface of normal glandular epithelia. The extra cellular VU-3C6 reacts with MUC1, a large transmembrane glycoprotein observed. Quantity: 1 ml domain of MUC1 largely consists of a highly conserved, O-glycosylated expressed on the ductal surface of normal glandular epithelia. The 20 amino acids tandem repeat which can occur 30-100 times per extra cellular domain of MUC1 largely consists of a highly conserved, Use: IP, F, P molecule depending on the length of the allele involved. In the vast O-glycosylated 20 amino acids tandem repeat which can occur 30-100 Quantity: 1ml (MON 7006-1) Milk fat globulin majority of human carcinomas this protein is up-regulated and poorly times per molecule depending on the length of the allele involved. In 5 ml (MON 7006-5) glycosylated and appears on the cell surface in a non-polarized fashion. the vast majority of human carcinomas this protein is up-regulated The dominant epitope of 139H2 has not yet been determined. Source: and poorly glycosylated and appears on the cell surface in a non- Cat.no. MON 2060 (Monoclonal) A Balb/c mouse was immunized with deglycosylated purified MUC1 polarized fashion. The dominant epitope of mAb VU-3C6 is the 12-mer Melanoma-associated antigen glycoprotein. Splenocytes were fused with SP2/0 mouse myeloma cells. GVTSAPDTRPAP of the MUC1 tandem repeat as established with Clone MFG-06 “epitope fingerprinting”. Source: A Balb/c mouse was immunized with This antibody recognizes 40-45 kDa glycoprotein in human milk fat Use: F, P, C, E live cells (107 cells/dose in PBS) of human breast cancer cell line Cat.no. MON 7005 (Monoclonal) globule membrane preparation. Quantity: 100 µg ZR-75-1. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by sub cloning four times. Clone NKI-M7 Use: IC, F, P, WB, E This antibody recognizes a molecular complex consisting of two bands Quantity: 100 µg Use: F, E with MW of 150 kD and 90 kD respectively (reduced 4 subunits 120 kD, Quantity: 100 µg 95 kD, 29 kD and 25 kD). The antibody reacts strongly with melanoma cells derived from cell lines and short term cultures and melanoma cells in frozen tissue sections. The antigen detected by this antibody is found to be associated with the adhesion, spreading and motility of human cultured melanoma cells. Cross reactions: The antibody reacts with a proportion of naevi and with endothelial cells of small vessels.

Use: F Quantity: 1 ml (MON 7005-1) 5 ml (MON 7005-5)

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MUC1 (polymorphic epithelial mucin) MUC1 (polymorphic epithelial mucin) MUC1 (polymorphic epithelial mucin) MUC1 (polymorphic epithelial mucin)

Cat.no. MON 6044 (Monoclonal) Cat.no. MON 6046 (Monoclonal) Cat.no. MON 6048 (Monoclonal) Cat.no. MON 6050 (Monoclonal)

Clone VU-3D1 Clone VU-11D1 Clone VU-12E1 Clone VU-2G7 VU-3D1 reacts with MUC1, a large transmembrane glycoprotein VU-11D1 reacts with MUC1, a large transmembrane glycoprotein VU-12E1 reacts with MUC1, a large transmembrane glycoprotein VU-2G7 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The expressed on the ductal surface of normal glandular epithelia. The expressed on the ductal surface of normal glandular epithelia. The expressed on the ductal surface of normal glandular epithelia. The extra cellular domain of MUC1 largely consists of a highly conserved, extra cellular domain of MUC1 largely consists of a highly conserved, extra cellular domain of MUC1 largely consists of a highly conserved, extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 O-glycosylated 20 amino acids tandem repeat which can occur 30-100 O-glycosylated 20 amino acids tandem repeat which can occur 30-100 O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In times per molecule depending on the length of the allele involved. In times per molecule depending on the length of the allele involved. In times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated the vast majority of human carcinomas this protein is up-regulated the vast majority of human carcinomas this protein is up-regulated the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non- and poorly glycosylated and appears on the cell surface in a non- and poorly glycosylated and appears on the cell surface in a non- and poorly glycosylated and appears on the cell surface in a non- polarized fashion. The dominant epitope of mAb VU3D1 is the 8-mer polarized fashion. The dominant epitope of mAb VU11D1 is the 7-mer polarized fashion. The dominant epitope of mAb VU12E1 is the 5-mer polarized fashion. The dominant epitope of VU-2G7 has not been SAPDTRPA of the MUC1 tandem repeat as established with “epitope TSAPDTR of the MUC1 tandem repeat as established with “epitope PDTRP of the MUC1 tandem repeat as established with “epitope established with “epitope fingerprinting”. Source: A Balb/c mouse was fingerprinting”. Source: A Balb/c mouse was immunized with live cells fingerprinting”. Source: A Balb/c mouse was immunized with live cells fingerprinting”. Source: A Balb/c mouse was immunized with live cells immunized with synthetically glycosylated MUC1 60mer tandem 7 7 7 (10 cells/dose in PBS) of human breast cancer cell line ZR-75-1. (10 cells/dose in PBS) of human breast cancer cell line ZR-75-1. (10 cells/dose in PBS) of human breast cancer cell line ZR-75-1. repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3-COOH, Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of conjugated to bovin serum albumin. Splenocytes were fused with the hybridomas was established by sub cloning four times. the hybridomas was established by sub cloning four times. the hybridomas was established by sub cloning four times. SP2/0 mouse myeloma cells.

Use: F, E Use: F. E Use: F, E Use, F, P, E Quantity: 100 µg Quantity: 100 µg Quantity: 100 µg Quantity: 100 µg

MUC1 (polymorphic epithelial mucin) MUC1 (polymorphic epithelial mucin) MUC1 (polymorphic epithelial mucin) MUC1 (polymorphic epithelial mucin)

Cat.no. MON 6045 (Monoclonal) Cat.no. MON 6047 (Monoclonal) Cat.no. MON 6049 (Monoclonal) Cat.no. MON 6051 (Monoclonal)

Clone VU-4H5 Clone VU-11E2 Clone VU-13F11 Clone VU-4C2 VU-4H5 reacts with MUC1, a large transmembrane glycoprotein VU-11E2 reacts with MUC1, a large transmembrane glycoprotein VU-13F11 reacts with MUC1, a large transmembrane glycoprotein VU-4C2 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The expressed on the ductal surface of normal glandular epithelia. The expressed on the ductal surface of normal glandular epithelia. The expressed on the ductal surface of normal glandular epithelia. The extra cellular domain of MUC1 largely consists of a highly conserved, extra cellular domain of MUC1 largely consists of a highly conserved, extra cellular domain of MUC1 largely consists of a highly conserved, extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 O-glycosylated 20 amino acids tandem repeat which can occur 30-100 O-glycosylated 20 amino acids tandem repeat which can occur 30-100 O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In times per molecule depending on the length of the allele involved. In times per molecule depending on the length of the allele involved. In times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated the vast majority of human carcinomas this protein is up-regulated the vast majority of human carcinomas this protein is up-regulated the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non- and poorly glycosylated and appears on the cell surface in a non- and poorly glycosylated and appears on the cell surface in a non- and poorly glycosylated and appears on the cell surface in a non- polarized fashion. The dominant epitope of mAb VU4H5 is APDTR as polarized fashion. The dominant epitope of mAb VU11E2 is the 7-mer polarized fashion. The dominant epitope of VU-13F11 has not yet been polarized fashion. The dominant epitope of VU-4C2 is APDTR as established with “epitope fingerprinting”. Source: A Balb/c mouse was TSAPDTR of the MUC1 tandem repeat as established with “epitope determined. Source: A Balb/c mouse was immunized with a MUC1 established with “epitope fingerprinting”. Source: A Balb/c mouse was

immunized with a MUC1 60mer tandem repeat NH2-(VTSAPDTRPAPG- fingerprinting”. Source: A Balb/c mouse was immunized with live cells 60mer tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH immunized with a MUC1 60mer tandem repeat NH2-(VTSAPDTRPAPG- 7 STAPPAHG)3-COOH conjugated to bovin serum albumin. Splenocytes (10 cells/dose in PBS) of human breast cancer cell line ZR-75-1. conjugated to bovin serum albumin. Splenocytes were fused with SP2/0 STAPPAHG)3-COOH conjugated to bovin serum albumin. Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas Splenocytes were fused with SP2/0 mouse myeloma cells. Stability of mouse myeloma cells. were fused with SP2/0 mouse myeloma cells. Stability of the hybridomas was established by sub cloning four times. the hybridomas was established by sub cloning four times. was established by sub cloning four times. Use: F, E Use: F, P, E Use: F, E Quantity: 100 µg Use: F, P, E Quantity: 100 µg Quantity: 100 µg Quantity: 100 µg

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MUC1 (polymorphic epithelial mucin) MUC5AC MUC5AC MUC5B, Sulfo-Lewisa

Cat.no. MON 6052 (Monoclonal) Cat.no. MON 6055 (Monoclonal) Cat.no. MON 6059 (Monoclonal) Cat.no. MON 1154 (Monoclonal)

Clone VU-5F12 Clone 1-13M1 Clone 58M1 Clone F2 VU-5F12 reacts with MUC1, a large transmembrane glycoprotein 1-13M1 reacts with peptide core of gastric mucin (MUC5AC). Source: 58M1 reacts with peptide core of gastric mucin (MUC5AC). Source: A The antibody F2 reacts in ELISA with the synthetically prepared sulfo- expressed on the ductal surface of normal glandular epithelia. The A BALB/c mouse was immunized with mucin isolated from an ovarian BALB/c mouse was immunized with mucin isolated from an ovarian Lewisa antigen and with the subpopulation of MUC5B (specific for extra cellular domain of MUC1 largely consists of a highly conserved, cyst fluid (pure endocervical type according to the Fenoglio’s cyst fluid (pure endocervical type according to the Fenoglio’s MUC5B), but not with Lewisa or sulfo-Lewisx antigens. The antibody

O-glycosylated 20 aminoacids tandem repeat which can occur 30-100 classification). Splenocytes were fused with mouse myeloma SP2/0 cells. classification). Splenocytes were fused with mouse myeloma SP2/0 cells. binds to isolated high Mr mucins, The epitope was mapped to the

times per molecule depending on the length of the allele involved. In SO3- 3Galβ1-3GlcNac-moiety of the Sulfo-Lea antigen. The F2 antibody

the vast majority of human carcinomas this protein is up-regulated and Use: F, P, E, WB Use: F, P, WB binds to high Mr salivary mucins, colon mucins, gallbladder mucins and

poorly glycosylated and appears on the cell surface in a non-polarized Quantity: 100 µg Quantity: 100 µg cervical mucins, but not to low Mr salivery mucins and gastric mucins. fashion. Source: A Balb/c mouse was immunized with a MUC1 60mer In immunohistological sections a positive reaction was found with a

tandem repeat NH2-(VTSAPDTRPAPGSTAPPAHG)3-COOH conjugated number of epithelia, including the (sero)mucous salivary glands, the to bovin serum albumin. Splenocytes were fused with SP2/0 mouse MUC5AC MUC5AC goblet cells of the colon, the lining epithelium of cervical and esophageal myeloma cells. Stability of the hybridomas was established by sub cloning glands, the suprabasal keratinocytes and Hassall’s corpuscles of the four times. thymus. In addition, positive reaction in flow cytometry was found Cat.no. MON 6056 (Monoclonal) Cat.no. MON 6060 (Monoclonal) with an ovarian carnicoma cell line. No staining was found in normal Use: F, P, E breast, pancreas, small intestine, spleen and lymph nodes. Normal Quantity: 100 µg Clone 2-11M1 Clone 9-13M1 gastric glands were negative, but gastric intestinal metaplastic glands 2-11M1 reacts with peptide core of gastric mucin (MUC5AC). Source: 9-13M1 reacts with peptide core of gastric mucin (MUC5AC). Source: were strongly stained with F2 antibody. A BALB/c mouse was immunized with mucin isolated from an ovarian A BALB/c mouse was immunized with mucin isolated from an ovarian In rat tissues, the F2 antibody labeled epithelial cells of the salivary MUC2 cyst fluid (pure endocervical type according to the Fenoglio’s cyst fluid (pure endocervical type according to the Fenoglio’s glands, colon and stomach. In addition to epithelial cells, extracellular classification). Splenocytes were fused with mouse myeloma SP2/0 cells. classification). Splenocytes were fused with mouse myeloma SP2/0 cells. matrix components in rat thymus and skin were labeled by the F2 antibody. No labeling of erythrocytes, lymphocytes or bone marrow Cat.no. MON 6053 (Monoclonal) Use: F, P, WB Use: F, P, E, WB cells was found by flow cytometry. Quantity: 100 µg Quantity: 100 µg The present data shows a tissue specific distribution of the F2-epitope Clone Ccp58 in cells from the epithelial lineage in human and rat. Reacts with colon gastric cancer cells, normal intestine, colon and salivary gland (immunoperoxidase staining); human colon cancer cell MUC5AC Use: IF, C, WB, E, P line LS174T by flow cytometry & Western blotting. Epitope defined as - Quantity: 1 ml GTQTP- (GlyThrGlnThrPro). Source: A BALB/c mouse was immunized with synthetic human MUC2 (MI-29) peptide (VNTR region). Splenocytes Cat.no. MON 6057 (Monoclonal) were fused with NS1. Myeloperoxidase (MPO) Clone 2-12M1 Use: F, C, E, WB 2-12M1 reacts with peptide core of gastric mucin (MUC5AC). Source: Quantity: 100 µg A BALB/c mouse was immunized with mucin isolated from an ovarian Cat. no. MON 3098 (Monoclonal) NEW cyst fluid (pure endocervical type according to the Fenoglio’s classification). Splenocytes were fused with mouse myeloma SP2/0 cells. Clone 266-6K1 MUC3 The monoclonal antibody 266-6K1 reacts with Myeloperoxidase (MPO), Use: F, P, WB a glycoprotein with an alpha2beta2 heteromultimer expressed in all Quantity: 100 µg cells of the myeloid linage. MPO is abundantly present in azurophilic Cat.no. MON 6054 (Monoclonal) granules of polymorphonuclear neutrophils. It is an important enzyme used during phagocytic lysis of engulfed foreign particles which takes Clone M3.1 MUC5AC part in the defense of the organism through production of hypochlorous M3.1 reacts with human mucin-3 (MUC3) antigen. Cancer cells of acid (HOCl), a potent oxidant. MPO is rapidly released by activated colon, breast and stomach; normal cells of salivary gland, breast, lung, polymorphonuclear neutrophils. Involvement of MPO has been described & G.I. tract.. Source: A BALB/c mouse was immunized with synthetic Cat.no. MON 6058 (Monoclonal) in numerous diseases such as atherosclerosis, lung cancer, Alzheimer’s human MUC3 (SIB-35) peptide. Splenocytes were fused with NS1. disease and multiple sclerosis. Autoimmune antibodies to MPO are Clone 45M1 involved in Wegeners disease. Since the discovery of MPO deficiency, Use: F, C, E, WB 45M1 reacts with peptide core of gastric mucin (MUC5AC). Source: A initially regarded as rare and restricted to patients suffering from severe Quantity: 100 µg BALB/c mouse was immunized with mucin isolated from an ovarian infections, MPO has more attracted clinical attention. In experimental cyst fluid (pure endocervical type according to the Fenoglio’s studies antibodies to MPO can be used for various purposes ranging classification). Splenocytes were fused with mouse myeloma SP2/0 cells. from flow cytometric analysis to detection of polymorphonuclear neutrophils in tissue sections. Use: F, P, WB Quantity: 100 µg Use: E, IP, WB, F Quantity: 100 µg

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Neu oncogene protein, C-erbB-2 p53 Prostatic Specific Acid Phosphatase, PAP PSA

Cat.no. MON 7014 (Monoclonal) Cat.no. MON 7015 (Monoclonal) Cat.no. MON 9009 (Monoclonal) Cat.no. MON 3081 (Monoclonal)

Clone CB11 Clone BP53.12 Clone 4LJ Clone A67-B/E3 C-erbB-2 oncogene codes for a membrane protein, Neu protein. In P53 protein is thought to act as a tumour suppressor gene. Mutation Specificity for the 52 kDa PAP protein was tested by ELISA, immuno- This antibody recognizes PSA as p30 in ELISA and Western blots. Source: several studies the amplifi-cation of C-erbB-2 oncogene in carcinomas of p53 may represent the most common genetic event in human blotting and immunochemistry. The antigen was isolated from human A BALB/c mouse was immunized with a p30 enriched preparation of has been reported. Over expression of the Neu oncogene protein can be malignancy. The over expression and accumulation of p53 in cell nucleus seminal plasma. Location of antigen, cytoplasm, extra cellular. human sperm plasma. Splenocytes were fused with X63-Ag8.653 demonstrated by this antibody. In immunoblotting the antibody reacts was reported for a number of human tumours, such as breast, lung myeloma cells. with a 185 kDa protein. Immunogen: Synthetic peptide corresponding and colon carcinomas. P53 over expression may be an useful tumour Use: F, P to a site on the internal domain of the C-erbB-2 oncoprotein. Antigen and prognostic marker. This antibody reacts with both wild and mutant Quantity: 1 ml Use: E, WB, P localization: Tumour cell membranes. p53. Species cross reactivity is restricted to primates. Immunogen: Quantity: 100 µg recombinant human p53 protein. Use: WB, F, P Proteinase inhibitor 9 Quantity: 1 ml Use: WB, IP, E, P, IC RECA-1, Rat Endothelial Cell Antigen Quantity: 100 µg Also available as FITC conjugate (MON7015F - 100 µg) Cat. no. MON 2065 (Monoclonal) NEW NIS, Na+/I- Symporter Cat.no. MON 6005 (Monoclonal) Clone PI9-17 p53 The human intracellular serpin proteinase inhibitor 9 (PI9) is the only Clone HIS-52 Cat.no. PS 265 (Polyclonal) human protein able to inhibit the activity of the serine protease The RECA-1 antibody has shown to be strictly specific for rat endothelial granzyme B. Granzyme B is expressed by cytotoxic lymphocytes and cells. It recognizes a cell-surface component that is still present after Immunogen: Synthetic peptide from rat NIS. Tested on rat, pig and human Cat.no. MON 7049 (Monoclonal) induces rapid target cell apoptosis. PI9-17 Mab was selected after collagenase or trypsin treatment. It reacts with rat heart, liver, kidney, tissues. Positive control: Frozen sections of rat thyroid. Absorption with immunization with full length recombinant PI9 produced in lung, brain, salivary gland, tongue, gut, skin, spleen, thymus, lymph 10-100 µg immunogen per ml diluted antiserum abolishes the staining. Clone Pab122 Escherichia coli. No cross reactivity with other, homologous serpins node, Peyer’s patch and aorta. No cross reactivity in various tested The p53 gene is located on human chromosome 17p13.1. Mutation (PI6, PI8 and PAI-2) was observed. species was observed other than rat. Immunogen used peripheral and Use: F and/or allelic loss of this gene is one of the causes of the occurrence of mesenteric lymph nodes preparation. Quantity: 50 µl a variety of mesenchymal and epithelial tumours. If it occurs in the Use: IC, C, WB germ line, such tumours run in families. P53 binds to a DNA consensus Quantity: 250 µg Use: F, IF, C sequence, the p53 response element, and it regulates normal cell growth Quantity: 1 ml Ovarium carcinoma antigen cycle events by activating transcription of genes, involved either in progression through the cycle, or causing arrest in G1 when the genome Proximal Nephrogenic Renal Antigen is damaged. In most transformed and tumour cells the concentration Renal cell carcinoma Cat.no. MON 9003 (Monoclonal) of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased Cat.no. MON 4045 (Monoclonal) Clone OV632 half-life (4 h) compared to that of the wild-type (20 min). P53 localizes Cat.no. MON 9007 (Monoclonal) This antibody stains 80% of non-mucinous primary and metastatic in the nucleus, but is detectable at the plasma membrane during mitosis Clone PN-15 (66.4.C2) ovarian cancers. It rarely stains non-gynecological malignancies using and when certain mutations modulate cytoplasmic/nuclear distribution. PN-15 reacts with a glycoprotein of 200 kD (gp200), present in proximal Clone RC38 standard immunohistochemical conditions. Source: A BALB/c mouse was immunized with recombinant human renal tubules. The antigen is carbohydrate in nature and retained in This antibody stains 97% of primary renal cell carcinomas, 70% of p53 protein. Splenocytes were fused with mouse myeloma formalin fixed paraffin embedded tissues. Other nornal tissues that metastatic renal cell cancers and proximal tubules in normal kidney. Use: F P3-X63/AG8.653 cells. display the antigen include breast, parathyroid glands and epididymis. It does not stain epithelial cells of non-renal cell malignancies using Quantity: 1 ml (MON 9003-1) Among renal carcinomas 93% of primary and 84% of metastatic standard immuno-histochemical conditions. 5 ml (MON 9003-5) Use: F, P, C, WB carcinomas are positive. Relatively few other tumour types are positive: Cross reactivities: Quantity: 100 µg breast cancers, teratocarcinomas and parathyroid adenomas. Protease - non renal cell carcinoma malignancies: not detectable digestion prior to antibody incubation enhances staining of tissue - endothelial cells in various tumors sections. Source: A BALB/c mouse was immunized with renal cortical Placental alkaline phosphatase tissue extract. Splenocytes were fused with mouse myeloma Use: F SP2/0Ag14 cells. Quantity: 1 ml (MON 9007-1) 5 ml (MON 9007-5) Cat. no. MON 2088 (Monoclonal) NEW Use: F, P, WB Quantity: 100 µg Clone H7E8 The antibody recognizes throphoblast-derived membrane-associated enzyme alkaline phosphatase. Cross reactive with Human, Other not tested

Use: WB, F, E Quantity: 100 µg

166 www.monosan.com For research use only. Not for drug, diagnostic or other use. 167 Tumour Markers Tumour Markers

S100A8/A9 (MRP-8/MRP-14), Calprotectin Small Cell Lung Carcinoma, SCLC, Small Cell Lung Carcinoma, SCLC, Vascular Endothelial Cadherin epithelial antigen neural and endocrine antigen Cat. no. MON 3094 (Monoclonal) NEW Cat.no. MON 7013 (Monoclonal) Cat.no. MON 7010 (Monoclonal) Cat.no. MON 7012 (Monoclonal) Clone 27E10 Clone BV9 The monoclonal antibody 27E10 is a unique antibody recognising an Clone MOC-31 Clone MOC-52 This monoclonal antibody (subclass IgG2a) binds to human VE-cadherin. epitope on the S100A8/A9 heterocomplex that is not exposed on the This antibody is reactive with lung cancer associated antigens and has This antibody is reactive with lung cancer associated antigens and has Human vascular endothelium cadherin is an endothelium-specific individual subunits S100A8 (MRP8) or S100A9 (MRP14). The calcium. been studied and categorized in different clusters of reactivity patterns been studied and categorized in different clusters of reactivity patterns cadherin localized at the intercellular junctions. binding, migration inhibitory factor-related proteins, MRP-8 (S100A8) during the First International Workshop on Small Cell Lung Cancer during the First International Workshop on Small Cell Lung Cancer and MRP-14 (S100A9) belong to the S100 protein family. The expression Antigens held in London in April 1987. MOC-31 reacts with most Antigens held in London in April 1987. MOC-552 stain neural and a Use: F, C, Inh., WB, IP of these proteins is largely confined to the cytosol of neutrophils and epithelia, and, in lung cancer, all lung carcinomas. The membrane- variable number of endocrine tissues and in the lung if reacts Quantity: 100 µg monocytes. The complex formation of these proteins is a calcium- associated proteins detected by MOC-31 appear to have an apparent preferentially with SCLC and carcinoids. Also a limited number of dependent process. The S100A8/A9 heterocomplex, also called MRP-8/ molecular weight of 35-40 kD. MOC-31 is not reactive with normal non-SCLC cases react with this antibody. These non-SCLC cases which MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic and malignant mesothelia and therefore is especially useful for the express neural/endocrine epitopes might form a separate subgroup. protein fraction of circulating polymorphonuclear granulocytes and is detection of carcinoma cells in ascites or pleural effucions. The membrane-associated proteins the MOC-21, MOC-32 and MOC-52 also found in monocytes, macrophages and ileal tissue eosinophils. (= Cluster I antibodies) antibodies have apparent molecular weights of Peripheral blood monocytes carry the antigen extra- and intracellularly, Use: F, WB, P about 145 kD. MOC-52 is reactive with almost al SCLC cases. neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, Quantity: 1 ml antifungal and immunomodulating and antiproliferative effects. Use: F Besides this it is a potent chemotactic factor for neutrophils. Plasma Quantity: 1 ml concentrations are elevated in diseases associated with increased Small Cell Lung Carcinoma, SCLC, neutrophil activity. Granulocytes terminate their existence after neural and endocrine antigen transmigration through the intestinal wall. Therefore calprotectin is also Thomsen-Friedenreich antigen detectable in faeces. The monoclonal antibody 27E10 can be used for early detection of Cat.no. MON 7011 (Monoclonal) (TF; asialo-glycophorin) inflammatory macrophages, for the characterisation of tumorous tissues and the monitoring of peripheral blood cell cultures. The antibody Clone MOC-32 Cat.no. MON 8060 (Monoclonal) 27E10 does not react with lymphocytes or platelets. 27E10 shows This antibody is reactive with lung cancer associated antigens and has cross-reaction with a subpopulation of macrophages of rhesus monkey. been studied and categorized in different clusters of reactivity patterns Clone A78-G/A7 during the First International Workshop on Small Cell Lung Cancer A78-G/A7 recognize the carbohydrate epitope Thomsen-Friedenreich Use: E, WB, IP, C, F, P Antigens held in London in April 1987. MOC-32 stain neural and a (asialo-glycophorin; Galβ1-3GalNAc ). It is specific for both anomeric Quantity: 100 µg variable number of endocrine tissues and in the lung if reacts forms of this disaccharide (TF-α and TF-β, including related structures preferentially with SCLC and carcinoids. Also a limited number of on glycolipids). Immunblotting with human acute myelogenous non-SCLC cases react with this antibody. These non-SCLC cases which leukaemia cells revealed a series of TF-active glycoproteins with a Small Cell Lung Carcinoma, SCLC, express neural/endocrine epitopes might form a separate subgroup. main band at about 155 kDa. It shows no cross reactivity with sialylated The membrane-associated proteins the MOC-21, MOC-32 and MOC-52 glycophorin. Source: BALB/c mice were immunized with neuraminidase- neural and endocrine antigen (= Cluster I antibodies) antibodies have apparent molecular weights of treated human red blood cells. The isolated splenocytes of the mice were about 145 kD. MOC-32 identifies a subgroup of SCLC. Lack of electro fused with the myeloma cell line X63-Ag8.653. Cat.no. MON 7009 (Monoclonal) reactivity with MOC-21 and MOC-32 correlates with bad response of the tumour to (re)induction chemotherapy. This finding may be taken Use: F, P, E, WB Clone MOC-21 into account in deciding whether an individual patient with (recurring) Quantity: 100 µg This antibody is reactive with lung cancer associated antigens and has SCLC should be given chemotherapy or not. been studied and categorized in different clusters of reactivity patterns during the First International Workshop on Small Cell Lung Cancer Use: F Thomsen-Friedenreich antigen Antigens held in London in April 1987. MOC-21 stain neural and a Quantity: 1 ml variable number of endocrine tissues and in the lung if reacts (TF; asialo-glycophorin) preferentially with SCLC and carcinoids. Also a limited number of non-SCLC cases react with this antibody. These non-SCLC cases which Cat.no. MON 8061 (Monoclonal) express neural/endocrine epitopes might form a separate subgroup. The membrane-associated proteins the MOC-21, MOC-32 and MOC-52 Clone A68-B/A11 (= Cluster I anti-bodies) antibodies have apparent molecular weights A68-B/A11 recognize the carbohydrate epitope Thomsen-Friedenreich of about 145 kD. MOC-21 identify a subgroup of SCLC (4). Lack of (asialo-glycophorin; GalNac-βGal). It has no cross reactivity with reactivity with MOC-21 and MOC-32 correlates with bad response of sialylated glycophorin. Source: BALB/c mice were immunized with the tumour to (re)induction chemotherapy. This finding may be taken neuraminidase-treated human red blood cells. The isolated splenocytes into account in deciding whether an individual patient with (recurring) of the mice were electro fused with the myeloma cell line X63-Ag8.653. SCLC should be given chemotherapy or not. Use: F, P, E, B Use: F, WB Quantity: 100 µg Quantity: 1 ml

168 For research use only. Not for drug, diagnostic or other use. www.monosan.com 169 Secondary Reagents Mouse Isotype Controls / Streptavidin

Immunogen Host Unconjugated antibody FITC conjugated antibody Peroxidase conjugated antibody Mouse Isotype Controls Cat.no. Cat.no. Cat.no. Human IgG Goat PS100 PS100F PS100P ISOTYPE FITC R-PE TRI-COLOR BIOTIN Human IgM Goat PS101 PS101F PS101P IgG1 clone MOPC-21 M101 M104 M106 M115 Human IgA Goat PS102 PS102F PS102P IGg2A clone 5-205 M2a01 M2a04 M2a06 M2a15 Human IgG, M, A Goat PS103 PS103F PS103P Human IgG Rabbit PS104 PS104F PS104P These mouse isotype controls can be used for use with single color Human IgM Rabbit PS105 PS105F PS105P CD markers. Human IgA Rabbit PS106 PS106F PS106P For use in flow cytometry, all antibodies are optimized to be used at a Human IgG, M, A Rabbit PS107 PS107F PS107P 6 maximum of 1 µg per 10 cells. Goat IgG, M, A Rabbit PS108 PS108F PS108P Mouse IgG, M, A Rabbit PS109 PS109F PS109P Format of FITC conjugated antibody: 0.5 ml with 50 µg in 2 times concentrated PBS, EIA-grade BSA and Rat IgG, M, A Rabbit PS110 PS110F PS110P 0.1% sodium azide. EIA grade BSA is added to bring final protein Guinea-Pig IgG, M, A Rabbit PS111 PS111F PS111P concentration to 4-5 mg/ml. Sheep IgG, M, A Rabbit PS112 PS112F PS112P Format of R-PE, TRI-COLOR and BIOTIN conjugates: Bovine IgG, M, A Rabbit PS113 PS113F PS113P 0.5 ml with 50 µg in PBS, EIA-grade BSA and 0.1% sodium azide. EIA Horse IgG, M, A Rabbit PS114 PS114F PS114P grade BSA is added to bring final protein concentration to 4-5 mg/ml. Chicken IgY Rabbit PS115 PS115F PS115P

Streptavidin Fluorochrome Conjugated

FLUOROCHROME FITC R-PE TRI-COLOR STREPTAVIDIN MSA1001 MSA1004 MSA1006

These fluochrome conjugated provide the optimum fluorochrome to Format of Streptavidin R-PE conjugated antibody: protein ration in flow cytometry. Any access dye has been removed by 0.5 ml with 150 µg streptavidin conjugate in PBS, EIA-grade BSA and column chromotography after which it has been filtered through a 0.1% sodium azide. EIA-grade BSA is added to bring final protein 0.22 micron filter. concentration to 4-5 mg/ml.

For use in flow cytometry, all conjugated are optimized to be used at Format of Streptavidin TRI-COLOR conjugated antibody: 0.05 – 0.2 µg per 106 cells in a 100 µl staining volumed. Optimal 1 ml with 100 µg streptavidin conjugate in PBS, EIA-grade BSA and quantity has to be determined for each assay. 0.1% sodium azide. EIA-grade BSA is added to bring final protein concentration to 4-5 mg/ml. Format of Streptavidin FITC conjugated antibody: 1 ml with 1 mg streptavidin FITC in 2 times concentrated PBS, EIA- grade BSA and 0.1% sodium azide. EIA-grade BSA is added to bring final protein concentration to 4-5 mg/ml.

170 www.monosan.com For research use only. Not for drug, diagnostic or other use. 171 Aluminium cryo tubes for storage in liquid nitrogen / TUF General Technical Instructions For Use

Aluminium cryo tubes for storage in Introduction: b. Indirect Immunoperoxidase staining: liquid nitrogen 1. 1 to 8 as above Monoclonal and polyclonal antibodies are used in a large variety of 9. Incubate with peroxidase labeled secondary antibody for 60 min. Specifications: technical procedures. Methods to be selected are dependent on the at 37°C. • Tight sealing specific demands and materials available. The methods given below may 10. Wash with PBS 3 x 10 min. • Autoclavable at +121ºC offer a basis, which can be adapted to local demands and available 11. Stain with Diaminobenzidin (DAB) solution (0.05% DAB, 50 mM

• Suitable for deepfreezing at –196ºC materials. See evt. for further technical help: Current Protocols in Tris/HCl pH 7.4, 0.01% H2O2 freshly prepared) 10 min. at room • Storage of tissue samples Immunology, ed J.E. Coligan et al., J. Wiley & sons, New York 1991, temperature. • With screwcap and rubber ring insert ISBN 0-471-52276-7. 12. Wash with running tap water, 3 min. • Supplied in bags of 100 tubes 13. Counterstain with Mayer’s hematoxylin or OsO4.

A. Membrane Immunofluorescence: Product Code Total Volume Filling Volume Height Diameter D / E. Methods for Frozen Sections: PA6003 3 ml 2 ml 23 mm 14.5 mm 1. Transfer 5x 105 cells in 25 µl to a 10 x 75 mm test tube for staining. (15/16”) (9/16”) 2. Add 25-50 µl of the diluted monoclonal antibody, mix and a. Indirect Immunofluorescence microscopy of indirect PA6015 15 ml 10 ml 29 mm 30 mm incubate for 30’ on ice. Immunoperoxidase staining of frozen sections (11/8”) (11/8”) 3. Wash twice with PBS containing 1% BSA and 0.02% NaN3. 1. 4-6 micron thick sections should be used. 4. Add 50 µl of an appropriate FITC/TRITC labeled anti Ig antibody, 2. Sections are thawed at room temperature and dried for 30 min. mix and incubate for 30’ on ice. 3. Tissue can be fixed in acetone.

5. Wash twice with PBS containing 1% BSA and 0.02% NaN3. 4. Wash with PBS 3 x 3 minutes. Proceed as descibed for cells 6. Resuspend the cells in 100 µl washing buffer and analyze in flow grown on coverslips (C). cytometry or mount in 90% glycerol, 10% 1 M Tris/HCl TUF (Target Unmasking Fluid) pH 8.0 and analyze by fluorescence microscopy. F. Methods for Cells on Coverslips: An ideal pretreatment for immunohistochemistry on Paraffin Sections Storage: B. Microcytotoxicity Testing: 3x concentrated. Closed bottles have a guaranteed shelf life at a. Indirect Immunofluorescence Microscopy: The action on paraffin sections is comparable to the pretreatment of room temp of 2 years. 1. The cells grown on coverslips are gently washed with PBS at Western blots (heating in SDS and mercapto-ethanol). Linearization of Working solutions have a guaranteed quality for 48 hours. 1. Mix 1 µl of a cell suspension (containing 5x105 - 106 cells/ml) room temperature. proteins by deproteination and denaturation unmasks antigenic epitopes. with 1 µl of the diluted monoclonal antibody in a Terasaki tray 2. Add cold (-20°C) methanol and transfer to 4°C for 5 min. After this pretreatment, a majority of monoclonal and polyclonal Precautions: under oil. 3. Transfer the coverslips into cold (-20°C) acetone in an acetone antibodies can be used for immunohistochemistry on paraffin sections. Do not swallow or inhale, TUF does NOT contain heavy metals. 2. Incubate for 60’ at room temperature. resistant dish for 2 min. 3. Add 5 µl of an appropriate rabbit complement. 4. Transfer to PBS, rinse a few times. Use: Procedure: 4. Incubate for 120’ at room temperature. 5. Incubate the coverslips with 100 µl monoclonal antibody diluted - To reactivate masked epitopes for moabs, in paraffin embedded 1. Mount tissue sections on organosilane coated slides. 5. Score cell death either by eosin exclusion or propidium iodide in PBS for 60 min. at 37°C. tissue sections. 2. Bake sections at 60ºC. staining. 6. Wash with PBS 3 x 10 min. - To enhance performance of moabs and poabs on paraffin/cryostat 3. Dewax sections in xylene and rehydrate in graded ethanol. 7. Incubated with FITC or TRITC labeled second antibody directed sections and cytological specimens. 4. Dilute TUF and heat up to 90ºC using a waterbath or incubator. against IgG at appropriated dilution. 5. Place the slides in a coplin jar filled with TUF and incubate for C. Methods for Cells grown on Coverslips: 8. Rinse 3 x 10 min. with PBS. Result: 10 min at 90°C. 9. Mount in 90% glycerol 10% 1 M Tris/HCl pH 8.0. All monoclonals and polyclonals tested so far showed optimal results 6. Leave to cool for 15 minutes. in dilutions similar to the usual cryostat section protocols. 7. Rinse in 2 changes of de-ionized water and TBS. a. Indirect immunofuorescence Microscopy: N.B. Fixation of the cells with formaldehyde or glutaraldehyde is not 8. Proceed with normal immunohistochemical staining. 1. The cells grown on coverslip are gently washed with PBS advisable because of possible destruction of structure and antigenicity. Preparation: (supplemented with Ca2+ and Mg2+) at room temperature. In order to reduce background staining, dilution of the antibody with Dilute the concentrated Target Unmasking Fluid 1:3 with de-ionized 2. The cells are fixed for 30 min. in 1-3% paraformaldehyde in PBS supplemented with 0.5% BSA and 0.2% gelatin is recommendable. water. PHEM buffer pH 6.9. 3. Transfer the coverslips into 0.5% Triton X-100. 3% paraformalde b. Indirect Immunoperoxidase staining: Delivery: hyde in PHEM buffer for 2 min. 1. 1 to 6 as above. bottle of 250 ml (Cat.no. T 10333) 4. Wash 3 times with PHEM buffer for 10 min. 7. Incubate with peroxidase labeled second antibody, 30-60 min. bottle of 1 liter (Cat.no. T 10333 L) 5. Incubate the coverslips 2 times 5 min. in 50 mM NH4Cl in PHEM. at 37°C. Composition PHEM buffer: 60 mM Pipes, 25 mM Hepes, 10 mM 8. Wash with PBS, 3 x 10 min.

EGTA 2 mM MgCI2, pH 6.9. 9. Stain with 3,3-diaminobenzidin (DAB) solution (0.05% DAB,

6. Wash with PBS 3 times for 15 min. 50 mM Tris/HCl pH 7.4, 0.01% H2O2 freshly prepared) during 7. Incubate the coverslips with 100 µl monoclonal antibody diluted 10 min. at room temperature. in PBS for 60 min. at 37°C. 10. Wash with running tap water, 3 min. 8. Wash 3 times for 10 min. with PBS. 11. Counterstain with Mayer’s hematoxylin or OsO4 9. Incubate with FITC or TRITC labeled second antibody for 60 min. at 37°C. 10. Rinse 3 x 10 min. with PBS

172 www.monosan.com For research use only. Not for drug, diagnostic or other use. 173 General Technical Instructions For Use General Technical Instructions For Use

Sodium Azide G. Three step Avidin-Biotin immuno- 12. Stain with 3,3-diaminobenzidin (DAB) solution, 10 min. at room peroxidase method for monoclonal temperature. A stocksolution of 0.5% DAB in 0.5 M Tris/HCl antibodies on frozen sections * (pH 7.4) can be made an stored frozed in the dark. Before use a Sodium Azide added to antibody solutions can be easily removed by quantity needed for staining can be thawed and diluted 10 x dialysis. In all situations, attention should be paid to the protein with water. The diluted DAB solution should be filtrated. Just concentration. Do not dilute antibody solution too much without adding

1. Cut frozen sections (5µm) before use H2O2 must be added to a final concentration of 0.01%. protective protein. 2. Dry overnight (56°C) 13. Wash with running tap water, 3 min. 3. Tissue is fixed in aceton (20 min./R.T.) 14. Counterstain with Mayer’s hematoxylin, 2 min. Dialysis can be performed by standard methods with dialysis tubing or

4. Pre-incubate for 5 min. with 2% NHS/0.1%NaN3 /PBS 15. Wash with running tap water, 2 min. for small samples by centrifugation over a Sephadex G25 Column. 5. Incubate for 60 min. with primary antibody 16. Dehydrate with increasing solutions of ethanol: 50%, 70%, 96%, (Use 1 ml syringe with 0.75 ml pre-swollen Sephadex G25. add e.g. 6. Wash (6-8 times) with PBS absolute, 3 min. each. 0.25 ml buffer with evt protective protein (e.g. 0.1% BSA) Spin at

7. Wash 5 minutes with 2% NHS/0.1% NaN3 /PBS 17. Clear with xylol, 3 x 3 min. 2000-3000 RPM, 1-2 minutes, in centrifuge tube, repeat 2 times. 8. Incubate for 30 min with biotinylated secundairy antibody 18. Mount with mounting medium (e.g. malinol). Add sample 0.1 ml, spin and use the dialysed sample. 9. Wash 10 min with 2% NHS/PBS (no sodiumazide!) 10. Incubate for 30 min. with avidin-biotin-peroxidase-complex (ABC) 11. Wash 5 min. with 2% NHS/PBS (no sodiumazide!) I. Immunoperoxidase test on sections: General storage of lyophilized antibodies: 12. Wash 5 min. in PBS

13. Incubate for 1 min. in H2O2/DAB solution 14. Short wash with PBS 1. Frozen sections should have been fixed in acetone for 10 min. Store all lyophilized products at 4°C ( temporarily storage up to 15. Short but firm wash in running tabwater 2. Incubation in antisera 40-60 min. Only if necessary: 2a - 10’, 20’ several weeks at room temperature will not substantially affect the

16. Incubate 5 min. in CuS04 solution or 30’ blocking of endogenous PO in methanol, 0.1% H2O2. antibody titer). 17. Short wash in running tabwater 2b - Wash in PBS 2 x 5 min. Lyophilized antibodies should be reconstituted as given for each 18. Counterstain with Mayer’s hematoxylin (1-3 min.) 3. Incubation in conjugate (e.g. peroxidase conjugated anti IgG), individual product. Store the reconstituted product at 4°C for about 19. Incubate in tabwater until sections are blue (approx. 20 min.) 30 min. 2 months. For longer storage it is in general advised to divide in small 20. Dehydrage and mount slides 4. Wash in PBS 2 x 5 min. aliquots, which should be stored either at -20°C for about 1 year or at 5. Incubation in AEC, 0.01% H2O2, 10 min. Preparation substrate: -70°C for several years. Do not dilute the antibody too much since this * This protocol was kindly provided by the department of pathology a. 5 mg AEC (3-amino-9-ehtylcarbazole) is solublized in 0.5 ml will hamper the titer. Dilute always with a buffered saline containing a from the PAMM foundation, Eindhoven, The Netherlands. For more DMF (di-methyl-fomamide). A glass (or acetone resistant stabilizer e.g. PBS + 0.1% BSA. Avoid repeated freezing and thawing detailed information please contact us. plastic) tube and pipet should be used! cycles as much as possible. For storage at 4°C the presence of an

b. add 9.5 ml. 0.05 m sodium acitate buffer, pH 4.9. antimicrobial agent is advised e.g. NaN3 for merthiolate.

c. add 5 µl 30% H2O2. H. Indirect immunoperoxidase staining on 6. Wash in demi water, 2 x 5 min. 7. Slightly countertain in hematoxylin e.g. 10 sec. General storage of antibodies in formalin fixed paraffin embedded tissues 8. Wash in tap water until sections are blue. 9. Mount in aquamont and examine by microscopy soluble form: 1. 4 micron thick sections should be used. 2. Dewax in xylol, 3 x 3 min. It is advised to store at 4°C for 2 months, at -20°C for about 1 year or 3. Rehydrate in decreasing grades of ethanol: absolute, 96%, 70%, J. Membrane immunofluorescence: at -70°C for several years. Avoid repeated freezing and thawing cycles 50%. 3 min.each. as much as possible. For longer storage it is in general advised to divide 4. Block endogenous peroxidase activity with freshly made 0.3% in small aliquots, which should be stored either at -20°C for about 1 6 H2O2 in methanol, 20 min. 1. Transfer 1 x 10 cells to each 10 x 75 mm tube for staining. year or at -70°C for several years. Do not dilute the antibody too much 5. Wash with PBS, 3 x 3 min. Only if proteolytic pretreatment is Pellet cells by centrifuging at 200 g x 5 min. (approx. 1000 r.p.m. since this will hamper the titer. Dilute always with a buffered saline required. in a normalcentrifuge). containing a stabilizer e.g. PBS + 0.1% BSA. For storage at 4°C the

a. Incubate sections with 0.1% Trypsin in 0.1% CaCl2 pH 7.6 for 2. Aspirate supernatant using a fine bore Pasteur pipet leaving the presence of an antimicrobial agent is advised e.g. NaN3 or merthiolate. 10 min. at room temperature. Wash with PBS, 3 x 3 min. cell pellet as dry as possible. 6. Cover the sections with 20% normal rabbit serum in PBS or normal 3. Add 50 µl of the diluted monoclonal antibody to each tube with human serum and incubate overnight in a humidity chamber at cells. Mix by tapping the tube and incubate for 20 min. on ice. room temperature to reduce non specific background staining. 4. Wash the cells 2 x PBS (plus Ca2+ and Mg2+) + 0.2% BSA and 7. Decant 20% normal rabbit serum. 0.1% sodium azide by centrifuging at 200 g x 5 min. After the 8. Incubate with monoclonal antibody (diluted in PBS), 1-2 hours at last wash, aspirate the supernatant using a fine bore Pasteur room temperature. pipet leaving the cell pellet as dry as possible. 9. Wash with PBS, 3 x 3 min. 5. Add 50 µl of the appropriate fluorochrome labeled anti Ig. 10. Incubate with peroxidase labeled second antibody, 30-60 min. at Mix by tapping the tube and incubate for 20 min. on ice. room temperature. 6. Wash the cells 2x in PBS + 0.2% BSA and 0.1% azide by 11. Wash with PBS, 3 x 3 min. centrifuging at 200 g x 5 min. Aspirate the supernatant leaving the cell pellet as dy as possible. 7. Resuspend the cells in 100 µl PBS-glycerol (1:1) and transfer 25 µl of the cell suspension to a frosted microscope slide and cover with coverslip. 8. Examine the slides by a fluorescence microscopy.

174 For research use only. Not for drug, diagnostic or other use. www.monosan.com 175 Product Name Index Product Name Index

Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page ACTH PS080 99 Blood group antigen A, clone HE-193 MON2044 96 CD8, clone BL-Ts8/2 MON1101 24 CD15, FITC conjugated, clone PM-81 MON1186F 29 ACTH, clone 2F6 MON3071 99 Blood group antigen A, clone HE-195 MON2045 96 CD8, clone FK18 MON1008 24 CD16, clone BL-LGL/1 MON1041A 29 Actin, clone 5F9F5 MON4001 135 Blood group antigen A1B, clone HE-24 MON2041 96 CD8, clone MEM-31 MON1102 24 CD16, clone BL-LGL/2 MON1041B 29 Actin, clone 647 MON4002 135 Blood group antigen ABH, clone HE-10 MON2040 96 CD8 FITC conjugated, clone MEM-31 MON1102F 24 CD16, clone BL-LGL/3 MON1041C 29 Albumin, clone A1-01 MON5024 145 Blood group antigen B, clone HEB-29 MON2042 96 CD8 R-PE conjugated, clone MEM-31 MON1102R 24 CD16, clone MEM-154 MON1029 29 Alkaline Phosphatase, clone V17.1 MON8040 83 Blood Group Antigen H, clone A70-A/A9 MON2046 96 CD8-antigen, rat, clone 15-11C5 MON2023 24 CD16, FITC conjugated, clone 3G8 MON1187F 29 Alpha Fetoprotein (AFP), clone C2 MON4035 153 Blood Group Lewis y, clone A70-C/C8 MON2047 96 CD8, T-Cell, clone WT82 MON1018 24 CD16, R-PE conjugated, clone 3G8 MON1187R 29 Alpha Fetoprotein (AFP), clone C3 MON4036 153 Bombesin PS089 99 CD9, clone MM2/57 MON1141 24 CD16, TC conjugated, clone 3G8 MON1187T 29 Alpha Fetoprotein (AFP), clone D10 MON4037 153 Bombesin/GRP PS223 99 CD9, Pre B-Cell, clone CLB-47 MON1113 25 CDw17, clone MEM-74 MON1107 29 Alpha Fetoprotein AFP, affinity purified PS076 154 Bovine IgG, IgM and IgA PS113 170 CD10, Biotinylated, clone 5-1B4 MON1184B 25 CD18, clone MEM-48 MON1110 30 Alpha v beta 3 Integrin, clone BV4 MON2033 3 Bovine IgG, IgM and IgA, FITC conjugated PS113F 170 CD10, CALLA, clone MEM-78 MON1103 25 CD18, FITC conjugated, clone CBL-LFA-1/1 MON1110F 30 Alpha v beta Integrin, clone BV3 MON2032 3 Bovine IgG, IgM and IgA, Peroxidase conjugated PS113P 170 CD10, clone BL-A11 MON1038 25 CD18, R-PE conjugated, clone CBL-LFA-1/1 MON1110R 30 Alpha-1-Antitrypsin NON-Z,clone F43.8.1 MON5039 145 BCRP (ABCG2), clone BXP-21 MON9041 132 CD10, FITC conjugated, clone 5-1B4 MON1184F 25 CD19, Biotinylated, clone SJ25-C1 MON1188B 30 Alpha-1-Antitrypsin, cloneF46.4.1 MON5037 145 BCRP (ABCG2), clone BXP-34 MON9037 132 CD10, R-PE conjugated, clone 5-1B4 MON1184R 25 CD19, clone BC3 MON1026 30 Alpha-1-Antitrypsin-Z, clone F50.4.1 MON5038 145 BCRP (ABCG2), clone BXP-9 MON9050 132 CD10, TC conjugated, clone 5-1B4 MON1184T 25 CD19, FITC conjugated, clone SJ25-C1 MON1188F 30 Alpha-B-crystallin PS094 137 BCRP, clone BXP-53 MON9051 132 CD11a, clone 87-6F9 MON1025 25 CD19, R-PE conjugated, clone SJ25-C1 MON1188R 30 Alpha-Catenin, clone 1G5 MON3083 3 Bromo-deoxy-Uridine, clone IIb5 MON8003 146 CD11a, clone BL-LEUK 11a MON1039 26 CD19, TC conjugated, clone SJ25-C1 MON1188T 30 Alpha-Fetoprotein (AFP), clone MBS-12 MON4034 153 Bromo-deoxy-Uridine/IdU, clone IU-4 MON8006 127, 146 CD11a, clone MEM-25 MON1104 9, 26 CD20, clone B-ly1 MON1178 30 Alpha-Fetoprotein, clone AFP-01 MON5032A 137, 153 Calcitonin PS081 100 CD11a, FITC conjugated, clone MEM-25 MON1104F 26 CD20, clone MEM-97 MON1111 30 Alpha-Fetoprotein, clone AFP-11 MON5032B 137, 153 Campylobacter jejuni, clone 57-24 MON2002 124 CD11a, LFA-1, clone MEM-83 MON1151 9, 26 CD20, FITC conjugated, clone H147 MON1189F 31 Alpha-MSH PS203 104 Capsaicin Receptor PS264 86, 137 CD11a, LFA-1, clone SPV-L7 MON1020 25 CD20, R-PE conjugated, clone HI47 MON1189R 31 Alpha-Tubulin, clone TU-02 MON4018 128 C.E.A., clone PARLAM4 MON9008 155 CD11a, R-PE conjugated, clone MEM-25 MON1104R 26 CD20, TC conjugated, clone HI47 MON1189T 31 Alpha-Tubulin, clone TU-16 MON4012 128 CAA MAM6, clone 115D8 MON9005 156 CD11b, clone BL-M/G 1 MON1105A 9, 27 CD21, C3d Receptor, clone 4C5 MON7019 31, 155 Alpha-Tubulin, TU-01 MON4009 128 CD1, clone RIV12 MON1022 21 CD11b, clone BL-M/G 2 MON1105B 9, 27 CD21, clone 21B9 MON3089 31 Aluminium cryo tubes + caps, 15 ml PA6015 172 CD1A, clone BL-THY 1 MON1061 21 CD11b, clone BL-M/G 4 MON1105C 9, 27 CD21, clone BL-B21/1 MON1112A 31 Aluminium cryo tubes + caps, 3 ml PA6003 172 CD1a, clone CBT6 MON1023 21 CD11b, CR3, clone Bear-1 MON1019 26 CD21, clone BL-B21/3 MON1112B 31 Amylin PS214 99 CD1b, clone 100-1A5 MON2039 21 CD11b, CR3, FITC conjugated, clone VIM12 MON1019F 26 CD21, clone NZ1 MON1028 31 Amyloid A, clone Reu-86.2 MON5065 145 CD2, clone BL-TP2/1 MON1066 21 CD11b, CR3, R-PE conjugated, clone VIM12 MON1019R 26 CD21, R-PE conjugated, clone BU-32 MON1112R 31 Angiotensin I PS073 99 CD3, clone BL-TP 3b MON1062 21 CD11c, clone BL-4H4 MON1106 27 CD21, TC, clone BU-32 MON1112T 31 Annexin V, clone UMC01 MON9020 146 CD3, clone MEM-57 MON1004 21 CD11c, clone FK24 MON1011 27 CD22 beta, B-cells, clone MEM-01 MON1067 32 Barmotin/7H6, clone 7H6 MON3054 4 CD3 FITC conjugated, clone MEM-57 MON1004F 22 CD11c, FITC conjugated, clone BU15 MON2106F 27 CD22, clone RFB4 MON1059 32 Basal Cell Cytokeratin, clone RCK103 MON3000 114 CD3 R-PE conjugated, clone MM-57 MON1004R 22 CD11c, R-PE conjugated, clone BU15 MON2106R 27 CD22, clone BL-3C4 MON1042 32 BasalKeratinocytes, clone PNK 1 MON3013 114 CD3, clone SPV-T3b MON1003 22 CD13, clone WM15 MON9054 27 CD22, FITC conjugated, clone RFB4 MON1042F 32 B-cell specific antigen, rat, clone 68-IB3 MON2024 116 CD3, clone WT32 MON1017 22 CD13, FITC conjugated, clone Tuk 1 MON1185F 27 CD22, R-PE conjugated, clone RFB4 MON1042R 32 B-cells, clone MB2 MON7026 116 CD4, clone BL-TH4 MON1036 22 CD13, R-PE conjugated, clone Tuk 1 MON1185R 27 CD22, TC conjugated, clone RFB4 MON1042T 32 Beta 1 Integrin, clone BV7 MON2031 3 CD4, clone MEM-115 MON1100 22 CD13, TC conjugated, clone Tuk 1 MON1185T 27 CD23, clone BL-C/B8 MON1043 32 Beta hCG, clone 5H4-E2 MON3067 100 CD4, clone MEM-241 MON2048 23 CD14, clone BL-4G7 MON1040 28 CD23, FITC conjugated, clone Tü 1 MON1043F 32 Beta III Tubulin, clone TU-20 MON4017 128 CD4 APC conjugated, clone MEM-241 MON2048A 23 CD14, clone MEM-18 MON1108 28 CD24, cl.CLB-134 MON1119 33 Beta-2 microglobulin, clone B2M-01 MON1031A 155 CD4 FITC conjugated, clone MEM-241 MON2048F 23 CD14, FITC conjugated, clone Tuk 4 MON1108F 28 CD25, clone BL-D/D1 MON1044A 33, 88 Beta-Catenin, clone 9F2 MON3055 3 CD4 R-PE conjugated, clone MEM-241 MON2048P 23 CD14, clone Tuk 4 MON1135 28 CD25, clone RO/G10 MON1044B 33, 88 Beta-Galactosidase, clone BG-02 MON5013 83 CD4, rat, clone 15-8A2 MON2021 22 CD14, R-PE conjugated, clone Tuk 4 MON1108R 28 CD25, FITC conjugated, cloneCD25-3G10 MON1190F 33 Beta-Glucuronidase, clone 105 MON5059 146 CD5, clone BL-TP 5a (6E3) MON1063 23 CD14, TC, clone Tuk 4 MON1108T 28 CD25, R-PE conjugated, cloneCD25-3G10 MON1190R 33 Beta-HCG, clone 2092 MON2001 101 CD5, clone BL-TP 5b (3F4) MON1064 23 CD14,Human monocyte, clone MEM-15 MON1072 28 CD25, TC conjugated, cloneCD25-3G10 MON1190T 33 Beta-Tubulin, cl.TU-06 MON4010 128 CD5-antigen, rat, clone 2-4-2B1 MON2022 23 CD15, clone BL-G15 MON1109 29 CD26, clone 202.36 MON1030 33 Biotin, clone Bio-8 MON5042 146 CD6, clone BL-TP 6a MON1037 23 CD15, clone BRA4F1 MON1027 28 CD27, clone CLB-27 MON1114 33 BLNK (SLP-65) PS280 17 CD7, clone WT1 MON1006 23 CD15, clone MEM-158 MON1182 28 CD27, clone LT-27 MON1162 33

176 For research use only. Not for drug, diagnostic or other use. www.monosan.com 177 Product Name Index Product Name Index

Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page CD28, clone 204.12 MON1079 34 CD44, R-PE conjugated, clone MEM-85 MON1123R 6, 41 CD57, HNK1, clone NK-1 MON1012 47 CD83, Biotinylated, clone HB15e MON4060B 53 CD28, clone YTH 913.12 MON1183 34 CD45, clone BL-LEUK 45 MON1049 41 CD58, clone BRIC5 MON1177 9, 48 CD83, clone HB15e MON4060 53 CD29, clone MEM-101A MON1075 34 CD45, clone MEM-28 MON1179 41 CD59 FITC conjugated, clone MEM-43 MON1136F 48 CD83, FITC conjugated, clone HB15e MON4060F 53 CD29, clone Moon-4 MON1078 34 CD45, FITC conjugated, clone H130 MON1194F 41 CD59, clone MEM-129 MON2086 48 CD83, R-PE conjugated, clone HB15e MON4060R 53 CD29, clone TDM29 MON1171 34 CD45, R-PE conjugated, clone H130 MON1194R 41 CD59, clone MEM-43 MON1136 48 CD84, clone 152-1D5 MON1077 54 CD30, clone Ki 1 MON1115 34 CD45, TC conjugated, clone H130 MON1194T 41 CD59, clone MEM-43/5 MON1157 48 CD84, clone 153-4D9 MON1005 54 CD31, clone CLB-HEC/75 MON1116 11, 35 CD45RA, clone BL-4D4 MON1050 41 CD60, clone M-T6004 MON4054 48 CD86, clone BU63 MON4041 54 CD31, clone VM64 MON1142 12, 35 CD45RA, clone MEM-56 MON1125 42 CD61 Biotinylated, clone CLB-thromb/1 MON7051B 13, 48 CD86, FITC conjugated, clone BU63 MON4041F 54 CD32, Biotinylated, clone AT10 MON4038B 35 CD45RA, FITC conjugated, clone MEM-56 MON1125F 42 CD61 FITC conjugated, clone CLB-thromb/1 MON7051F 13, 48 CD86, R-PE Conjugated, clone BU63 MON4041R 54 CD32, clone AT10 MON4038 35 CD45RA, R-PE conjugated, clone MEM-56 MON1125R 42 CD61, clone BL-E6 MON1051 13, 48, 95 CD87, uPAR, clone IIIB11 MON3095 54 CD32, FITC conjugated, clone AT10 MON4038F 35 CD45RB FITC conjugated, clone MEM-55 MON1153F 42 CD61, clone CRC54 MON1147 13, 49, 95 CD88, clone P12/1 MON4084 54 CD32, R-PE conjugated, clone C1KM5 MON1152R 35 CD45RB, clone MEM-143 MON2085 42 CD62, clone CLB/thromb/6 (c2) MON1137 11, 50 CD88, FITC conjugated, clone P12/1 MON4084F 54 CD33, FITC conjugated, cloneCD33-4D3 MON1191F 36 CD45RB, clone MEM-55 MON1153 42 CD62, P-selectin, clone CRC81 MON1148 11, 49 CD89, clone MIP8a MON4068 55 CD33, R-PE conjugated, cloneCD33-4D3 MON1191R 36 CD45RO, LCA, T-Cells, clone CLB-UCHL1 MON1124 42 CD62-E, Biotinylated, clone ENA1 MON6010B 5, 49 CD89, FITC conjugated, clone MIP8a MON4068F 55 CD33, TC conjugated, cloneCD33-4D3 MON1191T 36 CD46, clone MEM-258 MON2000 43 CD62-E, clone ENA1 MON6010 5, 49 CD89, R-PE conjugated, clone MIP8a MON4068R 55 CD34, Biotinylated, clone 581 MON1192B 36 CD46, MCP, clone J4-48 MON1134 42 CD62-P, R-PE conjugated, clone CRC81 MON1148R 11, 49 CD90, clone AF-9 MON2003 55 CD34, clone ICO-115 MON1198 36 CD47, clone BRIC126 MON1165 43 CD62E, clone ENA2 MON6011 49 CD91, Biotinylated, clone A2Mr alpha-2 MON4074F 55 CD34, clone MEC14.7 MON1159 36 CD48, clone MEM-102 MON1085 43 CD62l, clone FMC46 MON4039 49 CD91, clone A2Mr alpha-2 MON4074R 55 CD34, clone QBEND-10 MON1164 36 CD49a, clone TS2/7 MON4042 43 CD62l, FITC conjugated, clone FMC46 MON4039F 49 CD91, FITC conjugated, clone A2Mr alpha-2 MON4074 55 CD34, R-PE conjugated, clone 581 MON1192R 36 CD49b, clone AK7 MON1181 43 CD62l, R-PE conjugated, clone FMC46 MON4039R 49 CD91, R-PE conjugated, clone A2Mr alpha-2 MON4075B 64 CD35, C3b Receptor, CR1, clone E11 MON1117 37 CD49b, clone CLB-150 MON1126 43 CD63 FITC conjugated, clone MEM-259 MON9000F 50 CD94, Biotinylated, clone DX22 MON4096B 55 CD36, clone CLB-IVC7 MON1118 37 CD49c, clone MIKd2 MON1274 13, 44 CD63 R-PE conjugated, clone MEM-259 MON9000R 50 CD94, clone DX22 MON4096 55 CD36, clone FA6-152 MON3084 37 CD49c, clone VM-2 MON4067 44 CD63, clone CLB-180 MON1138 50 CD94, FITC conjugated, clone DX22 MON4096F 55 CD36, clone VM58 MON1143 37 CD49d, clone 44H6 MON1175 14, 44 CD63, clone MEM-259 MON9000 50 CD94, R-PE conjugated, clone DX22 MON4096R 55 CD37, clone IPO-24 MON1035 38 CD49e, clone CLB-705 MON1127 44 CD64, clone 10.1 MON1169 50 CD95 (FAS antigen), clone B-R18 MON2010 55 CD38, Biotinylated, clone HIT2 MON1193B 38 CD49f, clone 4F10 MON1176 7, 44 CD64, R-PE conjugated, clone 10.1 MON1195R 50 CD95 (FAS/APO-1), clone ICO-160 MON2038 56 CD38, clone BL-D2 MON1045 38 CD49f, clone CLB-701 MON1128 6, 44 CD66aCD, clone YTH71.3 MON4047 50 CD97, clone MEM-180 MON1197 56 CD38, FITC conjugated, clone HIT2 MON1193F 38 CD50, pan leucocyte, clone MEM-171 MON1068 45 CD66e, clone CLB-139 MON1139 51 CD98, clone IPO-T10 MON2013 56 CD38, TC conjugated, clone HIT2 MON1193T 38 CDw50, clone BL-LEUK 50 MON1129 45 CD67, clone BL-B7 MON1052 51 CD98, clone MEM-108 MON1070 56 CD39, clone HIM80 MON4026 38 CD51, clone 23C6 MON1167 45, 95 CD68, clone BL-A/D8 MON1053 51 CD99, clone H036-1.1 MON2015 56 CD40 FITC conjugated, clone HB14 MON2146F 38 CD51, clone NKI-MG MON1130 45, 95 CD69, clone BL-Ac/p26 MON1054 51 CD99, clone MEM-131 MON1158 56 CD40, clone BL-C4 MON1046 38 CD52, clone YTH 66.9HL MON1168 45 CDw70, clone BU69 MON4065 51 CD100, clone 133.1G6 MON2017 56 CD41a, clone CLB-48 MON1120 6, 39 CD53, clone MEM-53 MON1131 45 CD71 FITC conjugated, clone MEM75 MON1140F 52 CD101, clone BB27 MON4094 57 CD41a, clone CRC64 MON1145 12, 39 CD54, Biotinylated ICAM-1, clone HM-1 MON1016B 46 CD71 R-PE conjugated, clone MEM75 MON1140R 52 CD101, FITC conjugated, clone BB27 MON4094F 57 CD41a, clone HIP8 MON4027 39 CD54, clone HM-1 MON1016 7, 46 CD71, clone 66-IG10 MON1009 52, 93 CD101, R-PE conjugated, clone BB27 MON4094R 57 CD41a, clone M148 MON1173 12, 39 CD54, clone MEM 111 MON1132 7, 46 CD71, clone MEM75 MON1140 52, 93 CD102, clone B-T1 MON4044 57 CD41a, clone VM16a MON1144 13, 39 CD54, clone MEM 112 MON1133 7, 46 CD72, clone BL-A/A11 MON1056 52 CD103, clone LF61 MON4055 57 CD41b, clone HIP2 MON4028 40 CD54, FITC-conjugated, clone MEM-111 MON1132F 7, 46 CD75, Sialoprotein, clone LN-1 MON1166 52 CD103, FITC conjugated, clone LF61 MON4055F 57 CD42a, clone BL-H6 MON1047 40 CD54, R-PE-conjugated, clone MEM-111 MON1132R 7, 46 CDw76, clone BL-B8 MON1057 52 CD103, R-PE conjugated, clone LF61 MON4055R 57 CD42b, clone VM16d MON1146 12, 40 CD55, DAF, clone MEM-118 MON1155 46 CDw78, clone 60-3G2 MON1074 52 CD104, clone 450-9D MON4057 57 CD42d, clone CLB-SW16 MON1121 40 CD56, clone MEM-188 MON2072 46 CD79 Beta, clone AT107-2 MON4093 53 CD104, FITC conjugated, clone 450-9D MON4057F 57 CD43, clone BL-E/G3 MON1048 40 CD56, clone 123C3 MON9006 10, 47 CD79 Beta, FITC conjugated, clone AT107-2 MON4093F 53 CD105 FITC conjugated, clone MEM-226 MON2070F 58 CD43, clone MEM-59 MON1122 40 CD56, clone NKI-nbl-1 MON9012 10, 47 CD80, clone MEM-233 MON2071 53 CD105, clone MEM-226 MON2070 58 CD44, clone MEM-85 MON1123 6, 41 CD56, R-PE conjugated, clone MEM-188 MON2072R 46 CD80, FITC conjugated, clone MEM-233 MON2071F 53 CD105,Endoglin, clone E9 MON3091 57 CD44, clone NKI-P2 MON1150 6, 41 CD56, TC conjugated, clone MEM-188 MON2072T 46 CD81, clone 1.3.3.22 MON1076 53 CD105,Endoglin, clone PN-E2 MON6016 58 CD44, FITC conjugated, clone MEM-85 MON1123F 6, 41 CD57, clone 6-13-19-1 MON1010 47 CD82, clone B-L2 MON4053 53 CD106, VCAM-1, Biotinylated, clone 1G11B1 MON6017B 58

178 www.monosan.com For research use only. Not for drug, diagnostic or other use. 179 Product Name Index Product Name Index

Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page CD106, VCAM-1, clone 1G11B1 MON6017 13, 58 CD165, FITC conjugated, clone AD2-13H12 MON4070F 65 CGRP PS252 100 Dendritic Cells, clone RFD1 MON9501 116 CDw108, clone MEM-150 MON1163 58 CD165, R-PE conjugated, clone AD2-13H12 MON4070R 65 CGRP PS254 100 Desmin PS031 135 CD112, clone R2-525 MON4071 58 CD166, clone L50 MON1172 65 CGRP PS079 100 Desmin, clone D33 MON3001 135 CD114, clone LMM775 MON4058 58 CD173, clone BRIC231 MON4079 66 CGRP PS226 100 Desmoglein-1, clone 27B2 MON3056 4 CD114, R-PE conjugated, clone LMM775 MON4058R 58 CD174, clone A70-C/C8 MON4089 66 CGRP PS239 100 Desmoglein-2, clone 6D8 MON3057 4 CD116, R-PE conjugated, clone K12B7 MON4048R 58 CD176, clone A78-G/A7 MON4090 60 Chicken IgY and IgA PS115 170 Desmoglein-3, clone 5G11 MON3058 4 CD119, clone BB1E2 MON4056 59 CD177, clone MEM-166 MON2063 66 Chicken IgY, FITC conjugated PS115F 170 Dinitrophenyl Hapten, clone LO-DNP-2 MON5070 146 CD119, FITC conjugated, clone BB1E2 MON4056F 59 CD180, clone MHR73 MON4087 66 Chicken IgY, Peroxidase conjugated PS115P 170 DogCollagen I and III,affinity purified PS069 70 CD120a, Mouse PS055 60 CD180, FITC conjugated, clone MHR73 MON4087F 66 Chlamydia Trachomatis, clone 3 MON8004 124 Double Stranded DNA, clone AE-2 MON6030 15 CD120a PS053 59, 94 CD195, Biotinylated, clone HEK/1/85a MON4091B 66 Chromogranin A, clone LK2H10 MON9014 156 DR4, clone DR-4-02 MON3077 146 CD120a, clone H398 MON9058 59, 93 CD195, clone HEK/1/85a MON4091 66 Chromogranin A+B PS058 156 DR6, clone DR-6-04-EC MON3078 147 CD120a, clone H398, Biotinylated MON9058B 59, 93 CD195, FITC conjugated, clone HEK/1/85a MON4091F 66 Chromogranin A+B PS262 156 E.Coli 0-111, clone 3-12 MON2011 124 CD120b, clone80M2 MON9059 60, 95 CD195, R-PE conjugated, clone HEK/1/85a MON4091R 66 Citrate synthetase, clone 8D3-G4 MON2057 15 E.Coli K 1, clone 58-5 MON2004 124 CD120b, clone80M2, Biotinylated MON9059B 60 CD200, Biotinylated, clone OX-104 MON4073B 66 C-KIT (CD117) clone T595 MON1099 59 E-Cadherin, clone 5H9 MON7024 5 CD122, clone MIK-beta 1 MON4072 62 CD200, clone OX-104 MON4073 66 Collagen I PS041 70 E-Cadherin, clone 6F9 MON7022 5 CD122, FITC conjugated, clone MIK-beta 1 MON4072F 62 CD200, FITC conjugated, clone OX-104 MON4073F 66 Collagen I affinity purified PS047 70 Endothelial Differentiation Marker, clone 1F10 MON6013 157 CD130, clone B-T2 MON4043 62 CD200, R-PE conjugated, clone OX-104 MON4074B 55 Collagen I and III, Calf, affinity purified PS068 70 Endothelial Internalizing Surface Antigen, GES-HB MON6009 157 CD135, Biotinylated, clone BV10A4H2 MON4069B 62 CD200r, clone OX108 MON5095 67 Collagen I, affinity purified PS060 70 Endothelium, clone EN4 MON6002 157 CD135, clone BV10A4H2 MON4069 62 CD200r, FITC conjugated, clone OX108 MON5095F 67 Collagen I, II, III, clone MC1-HA MON4007 71 Endothelium, clone PAL-E MON6001 157 CD135, FITC conjugated, clone BV10A4H2 MON4069F 62 CD205, clone MG38 MON4099 67 Collagen I,II,III,IV,V PS046 71 Enkephalin PS202 101 CD135, R-PE conjugated, clone BV10A4H2 MON4069R 62 CD205, FITC conjugated, clone MG38 MON4099F 67 Collagen II PS042 71 Eosinophil Major Basic Protein, clone BMK-13 MON6008 147 CDw137, FITC conjugated, clone 4B4-1 MON4062F 62 CD205, R-PE conjugated, clone MG38 MON4099R 67 Collagen II affinity purified PS048 71 Ep-Cam (ESA), clone VU-1D9 MON7021 6 CDw137, clone 4B4-1 MON4062 62 CD206, clone 15-2 MON4088 67 Collagen II, affinity purified PS061 71 EPCR, clone LMR-42 MON9055 96 CDw137, R-PE conjugated, clone 4B4-1 MON4062R 62 CD210, Biotinylated, clone 3F9-2 MON4097B 67 Collagen III PS043 71 EGFR, cl. 2E9 MON1033 86 CD138, clone B-B4 MON5097 63 CD210, clone 3F9-2 MON4097 67 Collagen III affinity purified PS049 72 EGF, clone F20 MON8014 86 CD139, clone BU30 MON4066 63 CD210, R-PE conjugated, clone 3F9-2 MON4097R 67 Collagen III, affinity purified PS062 71 EGF, clone E5 MON8001 86 CD141, clone QBEND/40 MON5096 63 CD222, clone MEM-238 MON2064 67 Collagen III, clone MC3-HA MON4013 72 EGF, Mouse, clone F5 MON8002 86 CD143, Biotinylated, clone i2H5 MON4083B 63 CD226, Biotinylated, clone DX11 MON4098B 67 Collagen IV PS044 72 Epithelial Keratin Type I, clone AE-1 MON3037 115 CD143, FITC conjugated, clone i2H5 MON4083F 63 CD226, clone DX11 MON4098 67 Collagen IV PS057 72 Epithelial Keratin Type II, clone AE-3 MON3038 115 CD143, R-PE conjugated, clone i2H5 MON4083R 63 CD226, FITC conjugated, clone DX11 MON4098F 67 Collagen IV affinity purified PS050 73 Erythrocytes, clone SFL23.6 MON7050 97 CD146, clone OJ79c MON4086 63 CD226, R-PE conjugated, clone DX11 MON4098R 67 Collagen IV, affinity purified PS063 72 E-Selectin,CD62-E (Biotinylated) PS017 5, 49 CD146, FITC conjugated, clone OJ79c MON4086F 63 CD227, clone C595(NCRC48) MON4064 68 Collagen IV, clone 1042 MON4003 73 Extra Parotid Glycoprotein, clone 5E7 MON7027 120, 158 CD147, clone CB43 MON2034 63 CD227, FITC conjugated, clone C595(NCRC48) MON4064F 68 Collagen IV, clone MC4-HA MON4006 73 F VIII Related Antigen, KG7/30 MON6014 158 CD147, clone MEM-M6/1 MON1071 64 CD229, Biotinylated, clone HLy9.1.25 MON4085B 68 Collagen type V, clone MC5-HA MON4008 74 Fibrin/Fibrinogen, clone MFB-HB MON5019 97 CD150, Biotinylated, clone A12 MON4095B 64 CD229, clone HLy9.1.25 MON4085 68 Collagen V PS045 73 Fibronectin receptor, clone NKI-SAM1 MON1034 86 CD150, clone A12 MON4095 64 CD229, FITC conjugated, clone HLy9.1.25 MON4085F 68 Collagen V affinity purified PS051 73 Fibronectin, clone Fn-4 MON5064 97 CD150, FITC conjugated, clone A12 MON4095F 64 CD229, R-PE conjugated, clone HLy9.1.25 MON4085R 68 Collagen V, affinity purified PS064 73 FITC, clone F4/1 MON7070 148 CD152, clone BNI3 MON4063 64 CD230 (Aa109-112), clone 3F4 MON4092 68 Colon Carcinoma, clone CC-1 MON7007 156 Follicle Stimulating Hormone, clone 1038 MON5012 101 CD154, Biotinylated, clone MK13A4 MON4075F 64 CD235a, clone HIR2 MON4029 68 Colon Carcinoma, clone CC-3 MON7016 156 Galanin PS228 137 CD154, clone TRAP1.3.6 MON4059 64 CD236, clone BRIC100 MON4078 65 C-Peptide, clone C-PEP-01 MON5021 146 Gamma Interferon, clone F12 MON5093 77 CD154, FITC conjugated, clone MK13A4 MON4076 69 CD238, clone BRIC203 MON4081 68 Csk binding protein, clone PAG-C1 MON2081 17 Gamma Interferon, clone F14 MON5082 77 CD158b, clone GL183 MON4061 64 CD239, clone BRIC221 MON4077 68 Csk, clone CSK-04 MON3088 17 Gamma Light Chain, FITC conj., Mouse, clone LO-MK-1 MON5083F 108 CD161, clone LT161 MON9052 65 CD247, clone G3 MON4049 69 Csk-binding protein (PAG) PS279 17 Gamma Light Chain, Mouse, clone LO-MK-1 MON5083 108 CD162, clone TB5 MON9053 65 CD247, FITC conjugated, clone G3 MON4049F 69 Cytomegalovirus LA, clone BM219 MON8009 124 GAP-43 PS236 137 CD163, clone RM3/1 MON3096 65 CD247, R-PE conjugated, clone G3 MON4049R 69 DAXX, clone DAXX-01 MON5000 15 Gastrin PS250 101, 138 CD165, clone AD2-13H12 MON4070 65 CEA PS261 156 Dendritic cell, rat, clone IF119 MON2025 116 Gastrin PS085 101

180 For research use only. Not for drug, diagnostic or other use. www.monosan.com 181 Product Name Index Product Name Index

Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Gastrin/CCK PS220 102 HLA/DQ, clone BL-A/G3 MON1058 118 IFN Alpha 2B Recombinant, cloneIFNA2.3 MON5058 76 IgG, Peroxidase conjugated PS104P 170 GFAP PS032 138 HLA/DR R-PE conjugated, clone HL39 MON1082R 118 IFN Alpha 2B Recombinant, cloneIFNA2.4 MON5092 76 IgG, Peroxidase conjugated, clone EFE-565 MON5014P 107 GFAP, clone GFA-02 MON3028 138 HLA/DR, clone HL39 MON1082 118 IFN-gamma, clone F1 MON5071 77 IgG, Peroxidase conjugated, Mouse, clone LO-MG-7 MON5052P 108 GIP PS219 148 HLA-b, clone JOAN-1 MON1007 116 IFN-gamma, clone F3 MON5072 77 IgG1, FITC conjugated, Mouse, clone LO-MG1-2 MON5053F 108 GFAP, clone 6F2 MON3002 138 HLA-B27, clone EP-4 MON8039 116 IFN-Gamma PS019 77 IgG1, Mouse, Biotinylated, clone LO-MG1-2 MON5053B 108 GFAP, clone GF-01 MON3012 138 HLA-Class I, clone MEM-123 MON2079 117 Ig Lambda-chain, clone ICO-16 MON5047 109 IgG1, Mouse, clone LO-MG1-2 MON5053 108 Glicentin PS086 102 HLA-Class I, clone MEM-147 MON2078 117 IgA PS102 170 IgG1, R-PE, Mouse, clone LO-MG1-2 MON5053P 108 Glucagon PS248 102 HLA-D, clone 7.5.10.1 MON1013 117 IgA PS106 170 IgG2a, Biotinylated, Mouse, clone LO-MG2a-2 MON5054B 108 Glucagon PS087 102 HLA-D, clone BL-IA/6 MON1087 117 IgA, clone BFG-267 MON5004 107 IgG2a, Mouse, clone LO-MG2a-2 MON5054 108 Glucagon PS215 102 HLA-DQ1+DQ3, clone HL-37 MON1084 118 IgA, FITC conjugated PS102F 170 IgG2a, R-PE conjugated, Mouse, clone LO-MG2a-2 MON5054P 108 Glucagon/Glicentin PS221 102 HLA-DQw1, clone IIB3 MON1014 118 IgA, FITC conjugated PS106F 170 IgG2b, Biotinylated, Mouse, clone LO-MG2b-2 MON5055B 108 Glucose regulated protein 94, clone 9G10 MON3080 148 HLA-DR antigen, clone LN-3 MON1081 117 IgA, FITC conjugated, clone BFG-267 MON5004F 107 IgG2b, FITC conjugated, Mouse, clone LO-MG2b-2 MON5055F 108 Glycophorin, clone A63-B/C2 MON7045 97 HLA-DR+DP MON1002 117 IgA, FITC conjugated, Mouse, clone LO-MA-7 MON5050F 107 IgG2b, Mouse, clone LO-MG2b-2 MON5055 108 Glycophorin, clone A84-B/H2 MON7046 97 HLA-DR+DP, clone HL-40 MON1083 118 IgA, Mouse, Biotinylated, clone LO-MA-7 MON5050B 107 IgG2b, R-PE conjugated, Mouse clone LO-MG2b-2 MON5055P 108 GM-CSF, clone HP1-22E9 MON7033 75 HLA-DRw52, clone 7.3.19.1 MON1015 118 IgA, Mouse, clone LO-MA-7 MON5050 107 IgG3, Biotinylated, Mouse, clone LO-MG3-7 MON5056B 108 GM-CSF, clone MP1-13G6 MON7034 75 HLA-E, clone MEM-E/02 MON2077 119 IgA, Peroxidase conjugated PS102P 170 IgG3, FITC conjugated, Mouse, clone LO-MG3-7 MON5056F 108 GnRH receptor, clone A9E4 MON8054 87 HLA-E, clone MEM-E/06 MON2080 119 IgA, Peroxidase conjugated PS106P 170 IgG3, Mouse, clone LO-MG3-7 MON5056 108 GnRH Receptor, clone F1G4 MON8056 87 HLA-G Biotinylated, clone MEM-G/9 MON2075B 119 IgA, Peroxidase conjugated, clone BFG-267 MON5004P 107 IgG3, R-PE conjugated, Mouse, clone LO-MG3-7 MON5056P 108 Goat anti alpha Fetoprotein PS077 154 HLA-G FITC conjugated, clone MEM-G/11 MON2087F 119 IgE, Biotinylated, Mouse, clone LO-ME-3 MON5051B 107 IgG4, clone BL-G4/1 MON5034 108 Golgi zone, clone AE-6 MON8055 15 HLA-G FITC conjugated, clone MEM-G/9 MON2075F 119 IgE, clone BL-E9 MON5035 107 IgM PS101 170 Granzyme B, clone GrB-7 MON7029 116 HLA-G R-PE conjugated, clone MEM-G/9 MON2075R 119 IgE, FITC conjugated, Mouse, clone LO-ME-3 MON5051F 107 IgM PS105 170 Guinea Pig anti Adrenomedullin PS255 99 HLA-G, clone MEM-G/1 MON2076 119 IgE, mouse, clone LO-ME-3 MON5051 107 IgM, Biotinylated, Mouse, clone LO-MM-9 MON5057B 109 H Type 2, clone 19-OLE MON2035 148 HLA-G, clone MEM-G/11 MON2087 119 IgE, Peroxidase conjugated, Mouse, clone LO-ME-3 MON5051P 107 IgM, Chicken clone 408-5.1 MON5046 109 HDL Apolipoprotein AI,apo AI PS071 154 HLA-G, clone MEM-G/9 MON2075 119 IgG PS100 170 IgM, Chicken, IgG and IgA, clone 408-6.1 MON5044 109 HDL Apolipoprotein AI,apoAI PS072 154 Horse IgG, IgM and IgA PS114 170 IgG PS104 170 IgM, clone ICL-931 MON5015 109 HDL Apolipoprotein, Apo-AI, clone 1C5 MON5030 158 Horse IgG, IgM and IgA, FITC conjugated PS114F 170 IgG, Biotinylated, Mouse, clone LO-MG-7 MON5052B 108 IgM, FITC conjugated PS101F 170 Heat Shock Protein 27, clone G3.1 MON5091 158 Horse IgG, IgM and IgA, Peroxidase conj. PS114P 170 IgG, Chicken, clone 409-3.1 MON5045 107 IgM, FITC conjugated PS105F 170 Helicobacter pylori, clone 51-13 MON2007 124 HSP-60, clone LK-1 MON5063 159 IgG, clone EFE-565 MON5014 107 IgM, FITC conjugated, clone ICL-931 MON5015F 109 Helospectin PS238 103 HSP-60, clone LK-2 MON5062 158 IgG, FITC conjugated PS100F 170 IgM, FITC conjugated, Mouse clone LO-MM-9 MON5057F 109 Hepatitis B virus, clone HB3 MON6080 124 Human CA-19.9, clone 121SLE MON3060 155 IgG, FITC conjugated PS104F 170 IgM, Mouse, clone LO-MM-9 MON5057 109 Hepatitis B virus, clone HB5 MON6081 124 Human chorionic gonadoptropin, clone HCG-55 MON3062 101 IgG, FITC conjugated, clone EFE-565 MON5014F 107 IgM, Peroxidase conjugated PS101P 170 Hepatocytes, clone EP-1 MON2058 158 Human chorionic gonadotropin, clone HCG-60 MON3063 101 IgG, FITC conjugated, Mouse clone LO-MG-7 MON5052F 108 IgM, Peroxidase conjugated PS105P 170 Hepatocytes, clone EP-2 MON2059 158 Human chorionic gonadotropin, clone HCG-61 MON3064 101 IgG, IgM and IgA PS103 170 IgM, Peroxidase conjugated, clone ICL-931 MON5015P 109 Herpes simplex virus 1 and 2, clone T111 MON7080 125 Human Growth Hormone PS036 102 IgG, IgM and IgA PS107 170 IgY, Chicken, clone 7C2 MON5048 109 Herpes simplex virus type 1, clone T96 MON7081 125 Human Growth Hormone, clone GH-45 MON3061 102 IgG, IgM and IgA, FITC conjugated PS103F 170 IgY, Chicken, clone 7C2, Biotinylated MON5048B 109 Herpes simplex virus type 2, clone T303 MON7082 125 Human Ku Antigen, clone Ku15 MON5066 149 IgG, IgM and IgA, FITC conjugated PS107F 170 IgY, Chicken, clone 7C2, Peroxidase conjugated MON5048P 109 H-FABP, clone 66E2 MON2019 147 Human protein-tyrosine kinase, clone LCK-01 MON2056 84 IgG, IgM and IgA, FITC conj., Guinea-Pig PS111F 170 Immunoglobulins, clone HB63 MON5010 107 H-FABP, clone 66E2, Biotinylated MON2019B 147 Human thyroid stimulating hormone, clone TSH-116 MON3066 106 IgG, IgM and IgA, Goat PS108 170 Immunoglobulins, clone MH14/1 MON5007 107 H-FABP, clone 67D3 MON2020 147 Human thyroid stimulating hormone, clone TSH-51 MON3065 106 IgG, IgM and IgA, Guinea-Pig PS111 170 Immunoglobulins, clone MH16/1 MON5009 107 H-FABP, clone 67D3, Biotinylated MON2020B 147 Human WAF1/p21 protein, clone WA-1 MON7048 16 IgG, IgM and IgA, R-PE conj. PS103P 170 Immunoglobulins, clone MH25/1 MON5008 107 Histamine PS242 103 ICAM-1,CD54 (Biotinylated) PS018 7, 46 IgG, IgM and IgA, R-PE conj. PS107P 170 Insulin PS222 103 Histidine Decarboxylase PS260 138 IFN-Alpha (II), clone N27 MON5085 76 IgG, IgM and IgA, R-PE conj., Guinea-Pig PS111P 170 Insulin PS088 103 Histone H1, clone AE-4 MON3048 15 IFN-Alpha (II), clone N39 MON5089 76 IgG, IgM and IgA, FITC conjugated, Goat PS108F 170 Insulin, clone 2D11-H5 MON5094 103 HIV p24 protein, clone ND1 MON4052 125 IFN-Alpha, clone F18 MON5067 75 IgG, IgM and IgA, R-PE conj., Goat PS108P 170 Insulin, clone E2-E3 MON5088 103 HIV protease, clone 1696 MON4051 125 IFN Alpha 1, clone 2-48 MON5086 75 IgG, Mouse, clone LO-MG-7 MON5052 108 Insulin, clone IN-05 MON5023 103 HIV-1 gp120, clone CB-gp120 MON4050 125 IFN Alpha 1, clone 2-52 MON5087 75 IgG, Peroxidase conjugated PS100P 170 Insulin/Proinsulin PS240 104

182 www.monosan.com For research use only. Not for drug, diagnostic or other use. 183 Product Name Index Product Name Index

Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Interleukin-1 RII,CDw121b, clone 6G5 MON5084 61, 88 Keratin 6, clone Ks6.KA12 MON3070 110 LH-RH PS082 104 MPO, Mouse, clone 8F4 MON2074 149 Interleukin-1 receptor (p80), clone Reg21 MON5075 61, 87 Keratin 7 (46 kDa) and 17 (46kDa), clone C-46 MON3019 111 Lipid A, clone 26-5 MON2012 126 MRP1, cloneMRPm5 MON9019 129 Interleukin-1 Receptor type 1 (p80), clone D14e3 MON5078 61, 87 Keratin 7, clone OVTL12/30 MON3014 110 Liver FABP, clone K5A6 MON6024 147 MRP1, cloneMRPm6 MON9017 129 Interleukin-1 Receptor type 1 (p80), clone D1f3 MON5077 61, 87 Keratin 7, clone RCK105 MON3007 111 Liver FABP, clone K5A6, Biotinylated MON6024B 147 MRP1, cloneMRPr1 MON9018 129 Interleukin-1 Receptor type 1 (p80), clone Reg20 MON5076 61, 87 Keratin 8 and 18, clone NCL5D3 MON3009 111 Liver FABP, clone L2B10 MON6023 147 MRP14, clone 47-8D3 MON9042 121 Interleukin-1 RII PS029 62, 88 Keratin 8, clone C-43 MON3035 111 Liver FABP, clone L2B10, Biotinylated MON6023B 147 MRP2(ABCC2), clone M2II-12 MON9032 130 Interleukin-1 RII, clone 8.5 MON5079 61, 88 Keratin 8, clone C-51 MON3032 111 Liver FABP PS023 148 MRP2(ABCC2), clone M2III-6 MON9027 130 Interleukin-1ra3, clone 2D11 MON5080 88 Keratin 8, clone M20 MON3016 111 LRP/MVP, clone LMR5 MON9028 133 MRP2/cMOAT, clone M2III-5 MON9056 130 Interleukin-2, clone F16 MON5020 78 Keratin 9, clone Ks 9.70 and Ks 9.216 MON3075 111 LRP/MVP, clone MVP-37 MON9036 133 MRP3(ABCC3), clone M3II-9 MON9030 130 Interleukin-6 ps037 79 Keratin 10, clone DE-K10 MON3025 112 Macrophage Marker, clone 3A5 MON7023 120 MRP3, clone M3II-21 MON9031 130 Interleukin-6, clone 5E1 MON5022 78 Keratin 10, clone RKSE60 MON3010 112 Macrophage specific antigen, rat, clone F-6-J MON2027 120 MRP4 (ABCC4), clone M4I-10 MON9057 131 Interleukin-6, clone MP5-20F3 MON5073 78 Keratin 13, clone 1C7 MON3017 112 Macrophage, clone EP-3 MON7047 120 MRP4 (ABCC4), clone M4I-80 MON9069 131 Interleukin-6, clone 5E1 MON5022S 78 Keratin 13, clone 2D7 MON3018 112 Macrophages (86 kD ag), clone 25F9 MON3097 120 MRP5(ABCC5), clone M5I-1 MON9033 131 Interleukin 6, mouse, clone MP5-32C11 MON5017 78 Keratin 14, clone DE-SPK14 MON3074 112 Macrophages F4/80, clone BM8 MON3099 122 MRP5(ABCC5), clone M5II-54 MON9034 131 Interleukin-8 ps038 79 Keratin 14, clone RCK107 MON3043 112 Macrophages, clone LN-5 MON7044 120 MRP6(ABCC6), clone M6II-21 MON9048 131 Interleukin-8R1 (CXCR1),CDw128, clone NVR1 MON5049 62, 79 Keratin 17, clone Ks 17.E3 MON3073 113 Major Vault Protein, clone 1011 MON9043 133 MRP6(ABCC6), clone M6II-31 MON9049 132 Interleukin-8R1 (CXCR1),CDw128, clone NVR1, Biotinylated MON5049B 62 Keratin 18, clone C-04 MON3034 113 Mannose Receptor, Biotin, clone 137378 MON2073B 89 MRP6(ABCC6), clone M6II-7 MON9047 131 Interleukin-10, clone JES5-2A5 MON5074 79 Keratin 18, clone DA-7 MON3036 113 Mannose Receptor, clone 15-2 MON2073 89 MUC1, clone 126E7 MON6040 161 Interleukin-10 PS016 79 Keratin 18, clone DC-10 MON3023 113 MCP-1, clone ECE.2 MON7035 80 MUC1 , clone 139H2 MON6041 161 Interstinal Fatty Acid Binding Protein PS022 147 Keratin 18, clone Ks 18.04 MON3022 113 MCP-1, clone ECE.2, Biotinylated MON7035B 80 MUC1, clone S1D12D7 MON6042 161 Intestinal Alkaline Phosphatase,clone MIG-I15 MON5011 83 Keratin 18, clone M9 MON3006 114 MCP-1, MCAF, clone MNA1 MON7031 80 MUC1, clone VU-11D1 MON6046 162 Intra-acrosomal protein, clone Hs-14 MON8017 2 Keratin 18, clone RCK 106 MON3027 114 MDR Sampler Pack MON9200 134 MUC1, clone VU-11E2 MON6047 162 Intra-acrosomal protein, Mammalian, clone Ds-2 MON8019 2 Keratin 18, clone RGE 53 MON3026 113 MDR, cMOAT/MRP2, clone M2I-4 MON9026 129 MUC1, clone VU-12E1 MON6048 163 Intra-acrosomal protein, Mammalian, clone Hs-8 MON8016 2 Keratin 19, clone BA-17 MON3033 114 MDR1 P-gp (ABCB1), clone JSB-1 MON9011 129 MUC1, clone VU-13F11 MON6049 163 Intra-acrosomal protein,Mammalian, clone ACR.4 MON8015 2 Keratin 19, clone RCK108 MON3020 114 MDR3 P-glycoprotein, clone P3II-26 MON9035 129 MUC1, clone VU-2G7 MON6050 163 IP-10, clone 6D4 MON7036 80 Keratin Type I & II, clone AE1/AE3 MON3076 115 Melanoma Associated Antigen, clone NKI/beteb MON7006 160 MUC1, clone VU-3C6 MON6043 161 IP-10, clone 6D4, Biotinylated MON7036B 80 Keratins 4,5,6,8,10, 13 and 18, clone C-11 MON3039 114 Melanoma Associated Antigen, clone NKI/C3 MON7003 160 MUC1, clone VU-3D1 MON6044 162 Isotype control Mouse IgG1, BIOTIN M115 171 Kinesin, clone KN-01 MON4046 138 Melanoma Associated Antigen, clone NKI/M6 MON7004 160 MUC1, clone VU-4C2 MON6051 163 Isotype control Mouse IgG1, FITC M101 171 Klebsiella, clone 70-2 MON2006 126 Melanoma Associated Antigen, clone NKI/M7 MON7005 160 MUC1, clone VU-4H5 MON6045 162 Isotype control Mouse IgG1, R-PE M104 171 Lambda light chain, clone 48 MON5002 120 Melanoma Discr. Antigen, clone PAL-M1 MON7001 157 MUC1, clone VU-5F12 MON6052 164 Isotype control Mouse IgG1, TRI-COLOR M106 171 Laminin PS040 149 Melanoma Discr. Antigen, clone PAL-M2 MON7002 157 MUC2, clone Ccp58 MON6053 164 Isotype control Mouse IgG2a, BIOTIN M2A15 171 LAT, clone LAT-01 MON4023 17 Melanoma Marker, clone 2G12 MON7017 160 MUC3, clone M3.1 MON6054 164 Isotype control Mouse IgG2a, FITC M2A01 171 Lck-interacting molecule PS276 17 MHC Class II, rat, clone 53-5D2 MON2028 121 MUC5AC, clone 1-13M1 MON6055 164 Isotype control Mouse IgG2a, R-PE M2A04 171 Lck-interacting molecule, clone LIME-06 MON3079 18 Microtubule Associated Protein, clone MT01 MON5060 128 MUC5AC, clone 2-11M1 MON6056 164 Isotype control Mouse IgG2a, TRI-COLOR M2A06 171 Lck-interacting molecule, clone LIME-10 MON3082 18 Microtubules, clone AE-8 MON5090 128 MUC5AC, clone 2-12M1 MON6057 164 JAM-1, clone BV16 MON3052 8 Lck-interacting molecule PS277 18 Milk fat globulin, clone MFG-06 MON2060 160 MUC5AC, clone 45M1 MON6058 164 JAM-1, clone M.Ab.F11 MON3053 8 LDL Apolipoprotein E, apo E PS070 154 Monocyte Chematactic Protein-1 PS007 77 MUC5AC, clone 58M1 MON6059 165 JAM-1, Mouse, clone BV12 MON2069 8 LDL Apolipoprotein, Apo-B, clone12G10 MON5026 159 Motilin PS208 104, 138 MUC5AC, clone 9-13M1 MON6060 165 JAM-C, clone 18F26 MON4024 9 LDL Apolipoprotein, Apo-B, clone20 MON5028 159 Mouse IgG, IgM and IgA PS109 170 Murine MIP-1alpha PS097 137 Kappa light chain, clone 2B7 MON5001A-1 119 LDL Apolipoprotein, Apo-B, clone4C11 MON5027 159 Mouse IgG, IgM and IgA, FITC conjugated PS109F 170 MVP / LRP, clone 1014 MON9044 133 Kappa Light Chain, clone L1C1 MON8050 119 LDL Apolipoprotein, Apo-B, clone5F8 MON5025 159 Mouse IgG, IgM and IgA, R-PE conjugated PS109P 170 MVP / LRP, clone 1032 MON9046 133 Keratin PS033 110 LDL Apolipoprotein, Apo-E, clone3D12 MON5029 159 Mouse IgG1, clone CT6 MON9011C 134 MVP/ LRP, clone 1027 MON9045 133 Keratin 2e, clone Ks 2.342.7.1 MON3072 110 Leucocyte common antigen like, rat, clone 50-4B1 MON2026 120 Mouse IL-18 PS015 80 Myelin, clone 2B5 MON4004 139 Keratin 4, clone 6B10 MON3015 110 Leukemia cell line HL-60, clone IPO-M6 MON8035 97, 159 Mouse IL-6 PS014 79 Myeloid cells, clone BM-1 MON2036 121 Keratin 5 and 18, clone C-50 MON3029 110 Lewis A, clone 7LE MON3051 97 MouseTNF-alpha PS052 80 Myeloid Specific Antigen, clone BM-2 MON2037 121 Keratin 5+8, clone RCK102 MON3008 110 Lewis B, clone 2-25LE MON3047 98 MPO, clone 266-6K1 MON3098 165 Myeloid Specific Antigen, clone BM-3 MON2051 121

184 For research use only. Not for drug, diagnostic or other use. www.monosan.com 185 Product Name Index Product Name Index

Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Description Cat. no. Page Myeloid Specific Antigen, clone BM-4 MON2052 121 p53, clone BP53-12 MON7015 166 Rat Nitric oxide synthase PS059 140 Substance P PS093 142 Myeloid Specific Antigen, clone BM-5 MON2053 122 p53, clone Pab122 MON7049 166 Rat Pancreastatin PS241 105 Substance P PS225 142 Myosin, clone 414 MON4005 135 P55,50, clone 1108-1 MON7037 126 Rat Collagen I, affinity purified PS065 70 Substance P 4-11 PS210 142 Nectin-2, Mouse, clone 502-57 MON2092 10 p80NPM/ALK PS084 122 Rat Collagen III, affinity purified PS066 72 Sulfo-LewisA, clone F2 MON1154 98, 165 Nectin-3, clone 103-A1 MON4000 10 PACAP 38 PS234 104 Rat IFN-gamma PS006 77 Syk, clone SYK-01 MON2084 19 Neu-oncogen protein, C-erbB-2, clone CB11 MON7014 166 PACAP-related peptide (PRP) PS235 104 RBP, clone G4E4 MON7071 151 Synaptophysin, clone SY38 MON9013 143 Neural Cell Adhesion Molecule, clone RNL-1 MON9021 11 PAG/Cbp, clone MEM-255 MON4022 17, 18 Renal Cell Carcinoma, clone RC38 MON9007 167 Tangles, clone MIG-T4 MON6003 143 Neuro Specific Enolase, NSE, clone MIG-N3 MON9004 140 Pan T cells, rat, clone 15-16A1 MON2029 122 Ribonucleoprotein Particles, clone 58-15 MON8034 16 T-Cell receptor a/b complex, clone BL-A8 MON1080 22 Neurofilament, 160 kDa, clone RNF403 MON2099 139 Pancreas Polypeptide PS091 104 S. Aureus Peptidoglycan, clone 10-34 MON2009 127 T-Cell receptor, clone TRIM-04 MON4020 19 Neurofilament, 160 kDa, clone RNF406 MON2098 139 Pancreastatin PS205 105 S. epidermis, clone 17-5 MON2008 127 TCR, clone MEM-262 MON2090 20 Neurofilament, clone 2F11 MON3004 139 Pan-Keratin, clone 80 MON3003 114 S100A8/A9, clone 27E10 MON3094 168 Tenascin, clone T2H5 MON7025 152 Neurofilament, clone 403 MON3031 139 PCNA, clone PC10 MON6004 16 Salmonella Serogroup E, clone 42-77 MON2016 127 Thomsen-Friedenreich antigen, clone A68-B/A11 MON8061 169 Neurofilament, clone 402 MON3030 140 PECAM, clone BV8 MON1199 11, 35 Secretin PS092 105 Thomsen-Friedenreich antigen, clone A78-G/A7 MON8060 169 Neurofilament, clone NF-01 MON3011 139 PECAM-1, clone MEC7.46 MON1149 12, 35 Secretin PS217 105 Thy-1 (Ox-7 like), rat, clone 3-3B5 MON2030 55 Neurokinin A/SubstanceK PS232 139 PECAM-1, clone MEC7.46, Biotinylated MON1149B 12, 35 Secretory component, clone SC-05 MON3021 151 Thyroglobulin, clone 14/14 MON5003 106 Neuromedin U-8 PS206 170 Penicillin, clone Pen 9 MON4040 150 Secretory Leucocyte Protease Inhibitor PS024 151 TLR2 Biotinylated, clone mT2.7 MON4025B 91 Neurotensin PS090 140 Peroxidase Horse Radish, clone HP-3 MON5041 83 Serotonin PS010 142 TLR2 Biotinylated, clone T2.5 MON4019B 90 Neurotensin PS224 140 Phosphotyrosine, clone P9V6 MON2054 150 Serotonin (5-HT) PS233 142 TLR2, clone mT2.7 MON4025 91 Neutrofil Defensins 1-3, clone D21 MON2062 125, 149 Phosphotyrosine, clone P9V6, Biotinylated MON2054B 150 Serum Amyloid P, Biotinylated, clone 5.4D.3B MON6007 145 TLR2, clone T2.5 MON4019 90 Neutrophil Defensin 1-3, clone D21, Biotinylated MON2062B 125, 149 Phosphotyrosine, clone P-TYR-01 MON2055 151 Serum Amyloid P, clone 5.4A MON6006 145 TLR2, clone TL2.1 MON4030 89 Nitrotyrosine, clone HM11 MON9029 141 PigCollagen I and III,affinity purified PS067 70 Sheep anti HDL Apolipoprotein AI PS095 154 TLR2, clone TL2.1, Biotinylated MON4030B 89 Nitrotyrosine, clone HM11, Biotinylated MON9029B 141 Placental alkaline phosphatase, clone H7E8 MON2088 166 Sheep anti HDL Apolipoprotein AI PS096 155 TLR2, clone TL2.3 MON4031 90 NO-synthase PS243 141 Placental Alkine Phosphatase, clone MIG-P1 MON9002 83 Sheep IgG, IgM and IgA PS112 170 TLR2, clone TL2.3, Biotinylated MON4031B 90 NO-synthase PS257 141 Plakoglobin, clone 15F11 MON3059 12 Sheep IgG, IgM and IgA, FITC conjugated PS112F 170 TLR3, BiotinylatedcloneTLR3.7 MON3068B 91 NO-synthase PS258 141 PNMT PS256 150 Sheep IgG, IgM and IgA, Peroxidase conjugated PS112P 170 TLR3, cloneTLR3.7 MON3068 91 NP-5 (Neutrophil Defensin 5), clone R3 MON3046 150 Porcine Acrosin, clone ACR.2 MON9015 2 SHP2, clone SIT-01 MON4021 19 TLR4, clone HTA125 MON4033 91 NPY PS227 141 PP PS216 105 SLP-76 PS278 19 TLR4, clone HTA125, Biotinylated MON4033B 91 NPY 1-20 PS231 141 PPY PS229 150 SLP-76, clone SLP-76/03 MON3085 19 TLR4/MD2, Mouse, clone MTS510 MON4032 91 NSP-A and NSP-B, clone RNL-2 MON2095 141 Prepro-NPY 68-97 (C-PON) PS230 151 SLPI, clone 31 MON2005 151 TLR4/MD2, Mouse, clone MTS510, Biotinylated MON4032B 91 NSP-A and NSP-B, clone RNL-3 MON2096 141 Progesterone, clone Pg.13 MON2049 105 SLPI, clone 31, Biotinylated MON2005B 151 TLR9, Biotinylated, clone 5G5 MON2093B 92 NSP-C, clone RNL-4 MON2097 142 Progesterone, clone Pg.53 MON2050 105 Small Cell Carcinoma, cl.MOC-31 MON7010 168 TLR9, clone 5G5 MON2093 92 NTAL, clone NAP-03 MON2082 18 Prostatic Specific Acid Phosphatase, clone 4L MON9009 18 Small Cell Carcinoma, MOC-21 MON7009 168 TLR9, Mouse clone 5G5 MON2068 92 NTAL, clone NAP-07 MON2083 18 Proteinase Inhibitor 9, clone Pi9-17 MON2065 122, 167 Small Cell Carcinoma, MOC-32 MON7011 168 TLR9, Mouse, Biotinylated clone 5G5 MON2068B 92 Nuclear antigen 1, clone E1-2.5 MON6035 126 Proteus spp, clone 31-17 MON2018 126 Small Cell Carcinoma, MOC-52 MON7012 169 T-lymphocytes, T4, clone B-F5 MON1024 123 Nuclear Membrane, clone AE-5 MON7061 15 Proximal Nephrogenic Renal Antigen, clone PN-15 MON4045 167 Small Lung Cell Carcinoma, clone MOC-1 MON9010 47 TNF receptor, p55, clone MR1-2, Biotinylated MON9062B 59, 94 NuMa, clone A73-B/D12 MON6029 15 PSA, clone A67-B/E3 MON3081 167 Smoothelin, clone C6G MON3093 135 TNF-Alpha, clone 4C6-H8 MON7041 81 Ornithine decarboxylase PS244 83 Pseudomonas, clone 69-5 MON2014 126 Smoothelin, clone R4A MON2094 135 TNF-Alpha, clone 4H31 MON9061 81 Ornithine decarboxylase PS259 83, 142 PYY PS253 150 SOCS3, clone SO4 MON3090 19 TNF-Alpha, clone J1D9 MON7042 81 Ovarium Carcinoma Antigen, cloneOV632 MON9003 166 Rabies Virus, clone RAB-50 MON7060 126 Somatostatin PS083 105 TNF-Alpha, clone J2D10 MON7043 81 Oxytocin PS207 104 Rat CCK-A receptor PS263 86 Somatostatin PS204 106 TNF-Alpha, clone T1 MON9068 81 P105 (PANA), clone 2B3 MON7038 16 Rat Endothelial Cell Antigen, clone HIS-52 MON6005 167 STAT 1, clone SM1 MON2089 19 TNF-Alpha, clone T1, Biotinylated MON9068B 81 P110 glycoprotein, multidr.-res.rel. clone LRP-56 MON9016 132 Rat IgG, IgM and IgA PS110 170 Streptavidin, clone Strep-10 MON5043 152 TNF-Alpha, clone T3 MON9067 81 P193/VPARP, clone P193-10 MON9038 134 Rat IgG, IgM and IgA, FITC conjugated PS110F 170 Streptavidin, FITC MSA1001 171 TNF-Alpha, clone T3, Biotinylated MON9067B 81 P193/VPARP, clone P193-4 MON9039 134 Rat IgG, IgM and IgA, Peroxidase conjugated PS110P 170 Streptavidin, R-PE MSA1004 171 TNF-Alpha PS030 82 P193/VPARP, clone P193-6 MON9040 134 Rat Liver Fatty Acid Binding Protein PS021 148 Streptavidin, TC MSA1006 171 TNF-Alpha (TNF3), clone 52B83 MON5006 82 P34CDc-2 Nuclear Protein, clonePOH-1 MON7039 16 Rat NIS, Na+/I’ Symporter PS265 166 Substance P PS251 142 TNF-Alpha PS011 82

186 www.monosan.com For research use only. Not for drug, diagnostic or other use. 187 Product Name Index Product Code Index

Description Cat. no. Page Cat. no. Description Page Cat. no. Description Page TNF-Alpha, Mouse, clone V1q MON9060 82 M101 Isotype control Mouse IgG1, FITC 171 MON1037 CD6, clone BL-TP 6a 23 TNF-Receptor p75,CD120b, Biotinylated, clone MR2-1 MON9063B 60, 94 M104 Isotype control Mouse IgG1, R-PE 171 MON1038 CD10, clone BL-A11 25 TNF-RI (p55/p60), Mouse clone HM14 MON9064 59, 93 M106 Isotype control Mouse IgG1, TRI-COLOR 171 MON1039 CD11a, clone BL-LEUK 11a 26 TNF-RI (p55/p60),CD120a, clone MR1-2 MON9062 59, 94 M115 Isotype control Mouse IgG1, BIOTIN 171 MON1040 CD14, clone BL-4G7 28 TNF-RII p75/p80, Mouse clone HM102 MON9065 60, 94 M2A01 Isotype control Mouse IgG2a, FITC 171 MON1041A CD16, clone BL-LGL/1 29 TNF-RII p75/p80,CD120b PS054 60, 94 M2A04 Isotype control Mouse IgG2a, R-PE 171 MON1041B CD16, clone BL-LGL/2 29 TNF-RII p75/p80,CD120b, clone MR37988 MON9063 60, 94 M2A06 Isotype control Mouse IgG2a, TRI-COLOR 171 MON1041C CD16, clone BL-LGL/3 29 TNF-RII p75/p80,CD120b, Mouse PS056 60, 95 M2A15 Isotype control Mouse IgG2a, BIOTIN 171 MON1042 CD22, clone BL-3C4 32 Transferrin receptor 1,CD71, clone 3B8 2A1 MON3086 51, 92 MON1002 HLA-DR+DP 117 MON1042F CD22, FITC conjugated, clone RFB4 32 Transferrin receptor 2, clone 9F8 1C11 MON3087 93 MON1003 CD3, clone SPV-T3bz 22 MON1042R CD22, R-PE conjugated, clone RFB4 32 Transferrin, clone HTF-14 MON5016 152 MON1004 CD3, clone MEM-57 21 MON1042T CD22, TC conjugated, clone RFB4 32 TUF Target Unmasking Fluid T 10333 172 MON1004F CD3 FITC conjugated, clone MEM-57 22 MON1043 CD23, clone BL-C/B8 32 TUF Target Unmasking Fluid T 10333 L 172 MON1004R CD3 R-PE conjugated, clone MM-57 22 MON1043F CD23, FITC conjugated, clone Tü 1 32 Urokinase, affinity purified PS075 85 MON1005 CD84, clone 153-4D9 54 MON1044A CD25, clone BL-D/D1 33, 88 Urokinase, affinity purified PS074 84 MON1006 CD7, clone WT1 23 MON1044B CD25, clone RO/G10 33, 88 Urokinase, clone PGM2001 MON5018 84 MON1007 HLA-b, clone JOAN-1 116 MON1045 CD38, clone BL-D2 38 Urokinase, clone PGM2005 MON5033 84 MON1008 CD8, clone FK18 24 MON1046 CD40, clone BL-C4 38 VAChT PS246 143 MON1009 CD71, clone 66-IG10 52, 93 MON1047 CD42a, clone BL-H6 40 VAChT PS211 143 MON1010 CD57, clone 6-13-19-1 47 MON1048 CD43, clone BL-E/G3 40 VAChT PS212 143 MON1011 CD11c, clone FK24 27 MON1049 CD45, clone BL-LEUK 45 41 Vascular Endothelial cadherin, clone BV9 MON7013 169 MON1012 CD57, HNK1, clone NK-1 47 MON1050 CD45RA, clone BL-4D4 41 Vesicular GABA Transporter (VGAT) PS237 143 MON1013 HLA-D, clone 7.5.10.1 117 MON1051 CD61, clone BL-E6 13, 48, 95 Vimentin, clone V9 MON3005 136 MON1014 HLA-DQw1, clone IIB3 118 MON1052 CD67, clone BL-B7 51 Vimentin, clone VI-RE/1 MON2091 136 MON1015 HLA-DRw52, clone 7.3.19.1 118 MON1053 CD68, clone BL-A/D8 51 VIP PS249 144 MON1016 CD54, ICAM-1, clone HM-1 7, 46 MON1054 CD69, clone BL-Ac/p26 51 VIP PS218 144 MON1016B CD54, Biotinylated ICAM-1, clone HM-1 46 MON1056 CD72, clone BL-A/A11 52 Vitronectin, clone BV1 MON3050 98 MON1017 CD3, clone WT32 22 MON1057 CDw76, clone BL-B8 52 VMAT2 PS247 143 MON1018 CD8, T-Cell, clone WT82 24 MON1058 HLA/DQ, clone BL-A/G3 118 VMAT2 PS213 143 MON1019 CD11b, CR3, clone Bear-1 26 MON1059 CD22, clone RFB4 32 MON1019F CD11b, CR3, FITC conjugated, clone VIM12 26 MON1061 CD1A, clone BL-THY 1 21 MON1019R CD11b, CR3, R-PE conjugated, clone VIM12 26 MON1062 CD3, clone BL-TP 3b 21 MON1020 CD11a, LFA-1, clone SPV-L7 25 MON1063 CD5, clone BL-TP 5a (6E3) 23 MON1022 CD1, clone RIV12 21 MON1064 CD5, clone BL-TP 5b (3F4) 23 MON1023 CD1a, clone CBT6 21 MON1066 CD2, clone BL-TP2/1 21 MON1024 T-lymphocytes, T4, clone B-F5 123 MON1067 CD22 beta, B-cells, clone MEM-01 32 MON1025 CD11a, clone 87-6F9 25 MON1068 CD50, pan leucocyte, clone MEM-171 45 MON1026 CD19, clone BC3 30 MON1070 CD98, clone MEM-108 56 MON1027 CD15, clone BRA4F1 28 MON1071 CD147, clone MEM-M6/1 64 MON1028 CD21, clone NZ1 31 MON1072 CD14, human monocyte, clone MEM-15 28 MON1029 CD16, clone MEM-154 29 MON1074 CDw78, clone 60-3G2 52 MON1030 CD26, clone 202.36 33 MON1075 CD29, clone MEM-101A 34 MON1031A Beta-2 microglobulin, clone B2M-01 155 MON1076 CD81, clone 1.3.3.22 53 MON1033 Epidermal Growth Factor Receptor,cl. 2E9 86 MON1077 CD84, clone 152-1D5 54 MON1034 Fibronectin receptor, clone NKI-SAM1 86 MON1078 CD29, clone Moon-4 34 MON1035 CD37, clone IPO-24 38 MON1079 CD28, clone 204.12 34 MON1036 CD4, clone BL-TH4 22 MON1080 T-Cell receptor a/b complex, clone BL-A8 22

188 For research use only. Not for drug, diagnostic or other use. www.monosan.com 189 Product Code Index Product Code Index

Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page MON1081 HLA-DR antigen, clone LN-3 117 MON1123F CD44, FITC conjugated, clone MEM-85 6, 41 MON1159 CD34, clone MEC14.7 36 MON1193B CD38, Biotinylated, clone HIT2 38 MON1082 HLA/DR, clone HL39 118 MON1123R CD44, R-PE conjugated, clone MEM-85 6, 41 MON1162 CD27, clone LT-27 33 MON1193F CD38, FITC conjugated, clone HIT2 38 MON1082R HLA/DR R-PE conjugated, clone HL39 118 MON1124 CD45RO, LCA, T-Cells, clone CLB-UCHL1 42 MON1163 CDw108, clone MEM-150 58 MON1193T CD38, TC conjugated, clone HIT2 38 MON1083 HLA-DR+DP, clone HL-40 118 MON1125 CD45RA, clone MEM-56 42 MON1164 CD34, clone QBEND-10 36 MON1194F CD45, FITC conjugated, clone H130 41 MON1084 HLA-DQ1+DQ3, clone HL-37 118 MON1125F CD45RA, FITC conjugated, clone MEM-56 42 MON1165 CD47, clone BRIC126 43 MON1194R CD45, R-PE conjugated, clone H130 41 MON1085 CD48, clone MEM-102 43 MON1125R CD45RA, R-PE conjugated, clone MEM-56 42 MON1166 CD75, Sialoprotein, clone LN-1 52 MON1194T CD45, TC conjugated, clone H130 41 MON1087 HLA-D, clone BL-IA/6 117 MON1126 CD49b, clone CLB-150 43 MON1167 CD51, clone 23C6 45, 95 MON1195R CD64, R-PE conjugated, clone 10.1 50 MON1099 C-KIT (CD117) clone T595 59 MON1127 CD49e, clone CLB-705 44 MON1168 CD52, clone YTH 66.9HL 45 MON1197 CD97, clone MEM-180 56 MON1100 CD4, clone MEM-115 22 MON1128 CD49f, clone CLB-701 6, 44 MON1169 CD64, clone 10.1 50 MON1198 CD34, clone ICO-115 36 MON1101 CD8, clone BL-Ts8/2 24 MON1129 CDw50, clone BL-LEUK 50 45 MON1171 CD29, clone TDM29 34 MON1199 PECAM (CD31) clone BV8 11, 35 MON1102 CD8, clone MEM-31 24 MON1130 CD51, clone NKI-MG 45, 95 MON1172 CD166, clone L50 65 MON1274 CD49c, clone MIKd2 13, 44 MON1102F CD8 FITC conjugated, clone MEM-31 24 MON1131 CD53, clone MEM-53 45 MON1173 CD41a, clone M148 12, 39 MON2000 CD46, clone MEM-258 43 MON1102R CD8 R-PE conjugated, clone MEM-31 24 MON1132 CD54, ICAM-1, clone MEM 111 7, 46 MON1175 CD49d, clone 44H6 14, 44 MON2001 beta-HCG, clone 2092 101 MON1103 CD10, CALLA, clone MEM-78 25 MON1132F CD54, ICAM-1, FITC-conjugated, clone MEM-111 7, 46 MON1176 CD49f, clone 4F10 7, 44 MON2002 Campylobacter jejuni, clone 57-24 124 MON1104 CD11a, clone MEM-25 9, 26 MON1132R CD54, ICAM-1, R-PE-conjugated, clone MEM-111 7, 46 MON1177 CD58, clone BRIC5 9, 48 MON2003 CD90, clone AF-9 55 MON1104F CD11a, FITC conjugated, clone MEM-25 26 MON1133 CD54, ICAM-1, clone MEM 112 7, 46 MON1178 CD20, clone B-ly1 30 MON2004 E. Coli K 1, clone 58-5 124 MON1104R CD11a, R-PE conjugated, clone MEM-25 26 MON1134 CD46, MCP, clone J4-48 42 MON1179 CD45, clone MEM-28 41 MON2005 SLPI, clone 31 151 MON1105A CD11b, clone BL-M/G 1 9, 27 MON1135 CD14, clone Tuk 4 28 MON1181 CD49b, clone AK7 43 MON2005B SLPI, clone 31, Biotinylated 151 MON1105B CD11b, clone BL-M/G 2 9, 27 MON1136 CD59, clone MEM-43 48 MON1182 CD15, clone MEM-158 28 MON2006 Klebsiella, clone 70-2 126 MON1105C CD11b, clone BL-M/G 4 9, 27 MON1136F CD59 FITC conjugated, clone MEM-43 48 MON1183 CD28, clone YTH 913.12 34 MON2007 Helicobacter pylori, clone 51-13 124 MON1106 CD11c, clone BL-4H4 27 MON1137 CD62, clone CLB/thromb/6 (c2) 11, 50 MON1184B CD10, Biotinylated, clone 5-1B4 25 MON2008 S. epidermis, clone 17-5 127 MON1107 CDw17, clone MEM-74 29 MON1138 CD63, clone CLB-180 50 MON1184F CD10, FITC conjugated, clone 5-1B4 25 MON2009 S. Aureus Peptidoglycan, clone 10-34 127 MON1108 CD14, clone MEM-18 28 MON1139 CD66e, clone CLB-139 51 MON1184R CD10, R-PE conjugated, clone 5-1B4 25 MON2010 CD95 (FAS antigen), clone B-R18 55 MON1108F CD14, FITC conjugated, clone Tuk 4 28 MON1140 CD71, clone MEM75 52, 93 MON1184T CD10, TC conjugated, clone 5-1B4 25 MON2011 E. coli 0-111, clone 3-12 124 MON1108R CD14, R-PE conjugated, clone Tuk 4 28 MON1140F CD71 FITC conjugated, clone MEM75 52 MON1185F CD13, FITC conjugated, clone Tuk 1 27 MON2012 Lipid A, clone 26-5 126 MON1108T CD14, TC, clone Tuk 4 28 MON1140R CD71 R-PE conjugated, clone MEM75 52 MON1185R CD13, R-PE conjugated, clone Tuk 1 27 MON2013 CD98, clone IPO-T10 56 MON1109 CD15, clone BL-G15 29 MON1141 CD9, clone MM2/57 24 MON1185T CD13, TC conjugated, clone Tuk 1 27 MON2014 Pseudomonas, clone 69-5 126 MON1110 CD18, clone MEM-48 30 MON1142 CD31, clone VM64 12, 35 MON1186F CD15, FITC conjugated, clone PM-81 29 MON2015 CD99, clone H036-1.1 56 MON1110F CD18, FITC conjugated, clone CBL-LFA-1/1 30 MON1143 CD36, clone VM58 37 MON1187F CD16, FITC conjugated, clone 3G8 29 MON2016 Salmonella Serogroup E, clone 42-77 127 MON1110R CD18, R-PE conjugated, clone CBL-LFA-1/1 30 MON1144 CD41a, clone VM16a 13, 39 MON1187R CD16, R-PE conjugated, clone 3G8 29 MON2017 CD100, clone 133.1G6 56 MON1111 CD20, clone MEM-97 30 MON1145 CD41a, clone CRC64 12, 39 MON1187T CD16, TC conjugated, clone 3G8 29 MON2018 Proteus spp, clone 31-17 126 MON1112A CD21, clone BL-B21/1 31 MON1146 CD42b, clone VM16d 12, 40 MON1188B CD19, Biotinylated, clone SJ25-C1 30 MON2019 H-FABP, clone 66E2 147 MON1112B CD21, clone BL-B21/3 31 MON1147 CD61, clone CRC54 13, 49, 95 MON1188F CD19, FITC conjugated, clone SJ25-C1 30 MON2019B H-FABP, clone 66E2, Biotinylated 147 MON1112R CD21, R-PE conjugated, clone BU-32 31 MON1148 CD62, P-selectin, clone CRC81 11, 49 MON1188R CD19, R-PE conjugated, clone SJ25-C1 30 MON2020 H-FABP, clone 67D3 147 MON1112T CD21, TC, clone BU-32 31 MON1148R CD62-P, R-PE conjugated, clone CRC81 11, 49 MON1188T CD19, TC conjugated, clone SJ25-C1 30 MON2020B H-FABP, clone 67D3, Biotinylated 147 MON1113 CD9, clone CLB-47 25 MON1149 PECAM-1, clone MEC7.46 12, 35 MON1189F CD20, FITC conjugated, clone H147 31 MON2021 CD4-antigen, rat, clone 15-8A2 22 MON1114 CD27, clone CLB-27 33 MON1149B PECAM-1, clone MEC7.46, Biotinylated 12 MON1189R CD20, R-PE conjugated, clone HI47 31 MON2022 CD5-antigen, rat, clone 2-4-2B1 23 MON1115 CD30, clone Ki 1 34 MON1150 CD44, clone NKI-P2 6, 41 MON1189T CD20, TC conjugated, clone HI47 31 MON2023 CD8-antigen, rat, clone 15-11C5 24 MON1116 CD31, clone CLB-HEC/75 11, 35 MON1151 CD11a, LFA-1, clone MEM-83 9, 26 MON1190F CD25, FITC conjugated, clone CD25-3G10 33 MON2024 B-cell specific antigen, rat, clone 68-IB3 116 MON1117 CD35, clone E11 37 MON1152R CD32, R-PE conjugated, clone C1KM5 35 MON1190R CD25, R-PE conjugated, clone CD25-3G10 33 MON2025 Dendritic cell specific antigen,rat, clone IF119 116 MON1118 CD36, clone CLB-IVC7 37 MON1153 CD45RB, clone MEM-55 42 MON1190T CD25, TC conjugated, clone CD25-3G10 33 MON2026 Leucocyte common antigen like, rat, clone 50-4B1 120 MON1119 CD24, cl.CLB-134 33 MON1153F CD45RB FITC conjugated, clone MEM-55 42 MON1191F CD33, FITC conjugated, clone CD33-4D3 36 MON2027 Macrophage specific antigen, rat, clone F-6-J 120 MON1120 CD41a, clone CLB-48 6, 39 MON1154 Sulfo-LewisA, clone F2 98, 165 MON1191R CD33, R-PE conjugated, clone CD33-4D3 36 MON2028 MHC Class II, rat, clone 53-5D2 121 MON1121 CD42d, clone CLB-SW16 40 MON1155 CD55, DAF, clone MEM-118 46 MON1191T CD33, TC conjugated, clone CD33-4D3 36 MON2029 Pan T cells, rat, clone 15-16A1 122 MON1122 CD43, clone MEM-59 40 MON1157 CD59, clone MEM-43/5 48 MON1192B CD34, Biotinylated, clone 581 36 MON2030 Thy-1 (Ox-7 like), rat, clone 3-3B5 55 MON1123 CD44, clone MEM-85 6, 41 MON1158 CD99, clone MEM-131 56 MON1192R CD34, R-PE conjugated, clone 581 36 MON2031 beta 1 Integrin, clone BV7 3

190 www.monosan.com For research use only. Not for drug, diagnostic or other use. 191 Product Code Index Product Code Index

Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page MON2032 alpha v beta Integrin, clone BV3 3 MON2072T CD56, TC conjugated, clone MEM-188 46 MON3009 Keratin 8 and 18, clone NCL5D3 111 MON3062 hCG, clone HCG-55 101 MON2033 alpha v beta 3 Integrin, clone BV4 3 MON2073 Mannose Receptor, clone 15-2 89 MON3010 Keratin 10, clone RKSE60 112 MON3063 hCG, clone HCG-60 101 MON2034 CD147, clone CB43 63 MON2073B Mannose Receptor, Biotin, clone 137378 89 MON3011 Neurofilament, clone NF-01 139 MON3064 hCG, clone HCG-61 101 MON2035 H Type 2, clone 19-OLE 148 MON2074 MPO, Mouse, clone 8F4 149 MON3012 Glial Fibrillary Acidic Protein, clone GF-01 138 MON3065 hTSH, clone TSH-51 106 MON2036 Myeloid cells, clone BM-1 121 MON2075 HLA-G, clone MEM-G/9 119 MON3013 Basal Keratinocytes, clone PNK 1 114 MON3066 hTSH, clone TSH-116 106 MON2037 Myeloid Specific Antigen, clone BM-2 121 MON2075F HLA-G FITC conjugated, clone MEM-G/9 119 MON3014 Keratin 7, clone OVTL12/30 110 MON3067 Beta hCG, clone 5H4-E2 100 MON2038 CD95 (FAS/APO-1), clone ICO-160 56 MON2075R HLA-G R-PE conjugated, clone MEM-G/9 119 MON3015 Keratin 4, clone 6B10 110 MON3068 TLR3, clone TLR3.7 91 MON2039 CD1b, clone 100-1A5 21 MON2075B HLA-G Biotinylated, clone MEM-G/9 119 MON3016 Keratin 8, clone M20 111 MON3068B TLR3, Biotinylated clone TLR3.7 91 MON2040 Blood group antigen ABH, clone HE-10 96 MON2076 HLA-G, clone MEM-G/1 119 MON3017 Keratin 13, clone 1C7 112 MON3070 Keratin 6, clone Ks6.KA12 110 MON2041 Blood group antigen A1B, clone HE-24 96 MON2077 HLA-E, clone MEM-E/02 119 MON3018 Keratin 13, clone 2D7 112 MON3071 ACTH, clone 2F6 99 MON2042 Blood group antigen B, clone HEB-29 96 MON2078 HLA-Class I, clone MEM-147 117 MON3019 Keratin 7 (46 kDa) and 17 (46kDa), clone C-46 111 MON3072 Keratin 2e, clone Ks 2.342.7.1 110 MON2044 Blood group antigen A, clone HE-193 96 MON2079 HLA-Class I, clone MEM-123 117 MON3020 Keratin 19, clone RCK108 114 MON3073 Keratin 17, clone Ks 17.E3 113 MON2045 Blood group antigen A, clone HE-195 96 MON2080 HLA-E, clone MEM-E/06 119 MON3021 Secretory component, clone SC-05 151 MON3074 Keratin 14, clone DE-SPK14 112 MON2046 Blood Group Antigen H, clone A70-A/A9 96 MON2081 Csk binding protein, clone PAG-C1 17 MON3022 Keratin 18, clone Ks 18.04 113 MON3075 Keratin 9, clone Ks 9.70 and Ks 9.216 111 MON2047 Blood Group Lewis y, clone A70-C/C8 96 MON2082 NTAL, clone NAP-03 18 MON3023 Keratin 18, clone DC-10 113 MON3076 Keratin Type I & II, clone AE1/AE3 115 MON2048 CD4, clone MEM-241 23 MON2083 NTAL, clone NAP-07 18 MON3025 Keratin 10, clone DE-K10 112 MON3077 DR4, clone DR-4-02 146 MON2048F CD4 FITC conjugated, clone MEM-241 23 MON2084 Syk, clone SYK-01 19 MON3026 Keratin 18, clone RGE 53 113 MON3078 DR6, clone DR-6-04-EC 147 MON2048P CD4 R-PE conjugated, clone MEM-241 23 MON2085 CD45RB, clone MEM-143 42 MON3027 Keratin 18, clone RCK 106 114 MON3079 Lck-interacting molecule, clone LIME-06 18 MON2048A CD4 APC conjugated, clone MEM-241 23 MON2086 CD59, clone MEM-129 48 MON3028 GFAP, clone GFA-02 138 MON3080 Glucose regulated protein 94, clone 9G10 148 MON2049 Progesterone, clone Pg.13 105 MON2087 HLA-G, clone MEM-G/11 119 MON3029 Keratin 5 and 18, clone C-50 110 MON3081 PSA, clone A67-B/E3 167 MON2050 Progesterone, clone Pg.53 105 MON2087F HLA-G FITC conjugated, clone MEM-G/11 119 MON3030 Neurofilament, clone 402 140 MON3082 Lck-interacting molecule, clone LIME-10 18 MON2051 Myeloid Specific Antigen, clone BM-3 121 MON2088 Placental alkaline phosphatase, clone H7E8 166 MON3031 Neurofilament, clone 403 139 MON3083 α-Catenin, clone 1G5 3 MON2052 Myeloid Specific Antigen, clone BM-4 121 MON2089 STAT 1, clone SM1 19 MON3032 Keratin 8, clone C-51 111 MON3084 CD36, clone FA6-152 37 MON2053 Myeloid Specific Antigen, clone BM-5 122 MON2090 TCR, clone MEM-262 20 MON3033 Keratin 19, clone BA-17 114 MON3085 SLP-76, clone SLP-76/03 19 MON2054 Phosphotyrosine, clone P9V6 150 MON2091 Vimentin, clone VI-RE/1 136 MON3034 Keratin 18, clone C-04 113 MON3086 Transferrin receptor 1, CD71, clone 3B8 2A1 51, 92 MON2054B Phosphotyrosine, clone P9V6, Biotinylated 150 MON2092 Mouse Nectin-2, clone 502-57 10 MON3035 Keratin 8, clone C-43 111 MON3087 Transferrin receptor 2, clone 9F8 1C11 93 MON2055 phosphotyrosine, clone P-TYR-01 151 MON2093 TLR9, clone 5G5 92 MON3036 Keratin 18, clone DA-7 113 MON3088 Csk, clone CSK-04 17 MON2056 human Protein-tyrosine kinase, clone LCK-01 84 MON2093B TLR9, Biotinylated, clone 5G5 92 MON3037 Epithelial Keratin Type I, clone AE-1 115 MON3089 CD21, clone 21B9 31 MON2057 Citrate synthetase, clone 8D3-G4 15 MON2094 Smoothelin, clone R4A 135 MON3038 Epithelial Keratin Type II, clone AE-3 115 MON3090 SOCS3, clone SO4 19 MON2058 Hepatocytes, clone EP-1 158 MON2095 NSP-A and NSP-B, clone RNL-2 141 MON3039 Keratins 4,5,6,8,10, 13 and 18, clone C-11 114 MON3091 CD105, Endoglin, clone E9 57 MON2059 Hepatocytes, clone EP-2 158 MON2096 NSP-A and NSP-B, clone RNL-3 141 MON3043 Keratin 14, clone RCK107 112 MON3093 Smoothelin, clone C6G 135 MON2060 Milk fat globulin, clone MFG-06 160 MON2097 NSP-C, clone RNL-4 142 MON3046 NP-5 (Neutrophil Defensin 5), clone R3 150 MON3094 S100A8/A9, clone 27E10 168 MON2062 Neutrophil Defensins 1-3, clone D21 125, 149 MON2098 Neurofilament, 160 kDa, clone RNF406 139 MON3047 Lewis B, clone 2-25LE 98 MON3095 CD87, uPAR, clone IIIB11 54 MON2062B Neutrophil Defensin 1-3, clone D21, Biotinylated 125, 149 MON2099 Neurofilament, 160 kDa, clone RNF403 139 MON3048 Histone H1, clone AE-4 15 MON3096 CD163, clone RM3/1 65 MON2063 CD177, clone MEM-166 66 MON2106F CD11c, FITC conjugated, clone BU15 27 MON3050 Vitronectin, clone BV1 98 MON3097 Macrophages (86 kD ag), clone 25F9 120 MON2064 CD222, clone MEM-238 67 MON2106R CD11c, R-PE conjugated, clone BU15 27 MON3051 Lewis A, clone 7LE 97 MON3098 MPO, clone 266-6K1 165 MON2065 Proteinase Inhibitor 9, clone Pi9-17 122, 167 MON2146F CD40 FITC conjugated, clone HB14 38 MON3052 JAM-1, clone BV16 8 MON3099 Macrophages F4/80, clone BM8 122 MON2068 Mouse TLR9, clone 5G5 92 MON3000 Basal Cell Cytokeratin, clone RCK103 114 MON3053 JAM-1, clone M.Ab.F11 8 MON4000 Nectin-3, clone 103-A1 10 MON2068B Mouse TLR9, Biotinylated clone 5G5 92 MON3001 Desmin, clone D33 135 MON3054 Barmotin/7H6, clone 7H6 4 MON4001 Actin, clone 5F9F5 135 MON2069 Mouse JAM-1, clone BV12 8 MON3002 Glial Fibrillary Acidic Protein, clone 6F2 138 MON3055 beta-Catenin, clone 9F2 3 MON4002 Actin, clone 647 135 MON2070 CD105, clone MEM-226 58 MON3003 Pan-Keratin, clone 80 114 MON3056 Desmoglein-1, clone 27B2 4 MON4003 Collagen IV, clone 1042 73 MON2070F CD105 FITC conjugated, clone MEM-226 58 MON3004 Neurofilament, clone 2F11 139 MON3057 Desmoglein-2, clone 6D8 4 MON4004 Myelin, clone 2B5 139 MON2071 CD80, clone MEM-233 53 MON3005 Vimentin, clone V9 136 MON3058 Desmoglein-3, clone 5G11 4 MON4005 Myosin, clone 414 135 MON2071F CD80 FITC conjugated, clone MEM-233 53 MON3006 Keratin 18, clone M9 114 MON3059 Plakoglobin, clone 15F11 12 MON4006 Collagen IV, clone MC4-HA 73 MON2072 CD56, clone MEM-188 46 MON3007 Keratin 7, clone RCK105 111 MON3060 Human CA-19.9, clone 121SLE 155 MON4007 Collagen I, II, III, interstitial, clone MC1-HA 71 MON2072R CD56, R-PE conjugated, clone MEM-188 46 MON3008 Keratin 5+8, clone RCK102 110 MON3061 hGH, clone GH-45 102 MON4008 Collagen type V, clone MC5-HA 74

192 For research use only. Not for drug, diagnostic or other use. www.monosan.com 193 Product Code Index Product Code Index

Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page MON4009 Alpha-Tubulin, TU-01 128 MON4047 CD66aCD, clone YTH71.3 50 MON4072F CD122, FITC conjugated, clone MIK-beta 1 62 MON4096F CD94, FITC conjugated, clone DX22 55 MON4010 Beta-Tubulin, cl.TU-06 128 MON4048R CD116, R-PE conjugated, clone K12B7 58 MON4073 CD200, clone OX-104 66 MON4096R CD94, R-PE conjugated, clone DX22 55 MON4012 a-Tubulin, clone TU-16 128 MON4049 CD247, clone G3 69 MON4073B CD200, Biotinylated, clone OX-104 66 MON4097 CD210, clone 3F9-2 67 MON4013 Collagen III, clone MC3-HA 72 MON4049F CD247, FITC conjugated, clone G3 69 MON4073F CD200, FITC conjugated, clone OX-104 66 MON4097B CD210, Biotinylated, clone 3F9-2 67 MON4017 Beta III Tubulin, clone TU-20 128 MON4049R CD247, R-PE conjugated, clone G3 69 MON4074 CD91, FITC conjugated, clone A2Mr alpha-2 55 MON4097R CD210, R-PE conjugated, clone 3F9-2 67 MON4018 Alpha-Tubulin, clone TU-02 128 MON4050 HIV-1 gp120, clone CB-gp120 125 MON4074B CD200, R-PE conjugated, clone OX-104 55 MON4098 CD226, clone DX11 67 MON4019 TLR2, clone T2.5 90 MON4051 HIV protease, clone 1696 125 MON4074F CD91, Biotinylated, clone A2Mr alpha-2 55 MON4098B CD226, Biotinylated, clone DX11 67 MON4019B TLR2 Biotinylated, clone T2.5 90 MON4052 HIV p24 protein, clone ND1 125 MON4074R CD91, clone A2Mr alpha-2 55 MON4098F CD226, FITC conjugated, clone DX11 67 MON4020 T-Cell receptor, clone TRIM-04 19 MON4053 CD82, clone B-L2 53 MON4075B CD91, R-PE conjugated, clone A2Mr alpha-2 64 MON4098R CD226, R-PE conjugated, clone DX11 67 MON4021 SHP2, clone SIT-01 19 MON4054 CD60, clone M-T6004 48 MON4075F CD154, Biotinylated, clone MK13A4 64 MON4099 CD205, clone MG38 67 MON4022 PAG/Cbp, clone MEM-255 17, 18 MON4055 CD103, clone LF61 57 MON4076 CD154, FITC conjugated, clone MK13A4 69 MON4099F CD205, FITC conjugated, clone MG38 67 MON4023 LAT, clone LAT-01 17 MON4055F CD103, FITC conjugated, clone LF61 57 MON4077 CD239, clone BRIC221 68 MON4099R CD205, R-PE conjugated, clone MG38 67 MON4024 JAM-C, clone 18F26 9 MON4055R CD103, R-PE conjugated, clone LF61 57 MON4078 CD236, clone BRIC100 65 MON5000 DAXX, clone DAXX-01 15 MON4025 TLR2, clone mT2.7 91 MON4056 CD119, clone BB1E2 59 MON4079 CD173, clone BRIC231 66 MON5001A-1 Kappa light chain, clone 2B7 119 MON4025B TLR2 Biotinylated, clone mT2.7 91 MON4056F CD119, FITC conjugated, clone BB1E2 59 MON4081 CD238, clone BRIC203 68 MON5002 Lambda light chain, clone 48 120 MON4026 CD39, clone HIM80 38 MON4057 CD104, clone 450-9D 57 MON4083B CD143, Biotinylated, clone i2H5 63 MON5003 Thyroglobulin, clone 14/14 106 MON4027 CD41a, clone HIP8 39 MON4057F CD104, FITC conjugated, clone 450-9D 57 MON4083F CD143, FITC conjugated, clone i2H5 63 MON5004 IgA, clone BFG-267 107 MON4028 CD41b, clone HIP2 40 MON4058 CD114, clone LMM775 58 MON4083R CD143, R-PE conjugated, clone i2H5 63 MON5004F IgA, FITC conjugated, clone BFG-267 107 MON4029 CD235a, clone HIR2 68 MON4058R CD114, R-PE conjugated, clone LMM775 58 MON4084 CD88, clone P12/1 54 MON5004P IgA, Peroxidase conjugated, clone BFG-267 107 MON4030 TLR2, clone TL2.1 89 MON4059 CD154, clone TRAP1.3.6 64 MON4084F CD88, FITC conjugated, clone P12/1 54 MON5006 Tumor Necrosis factor Alpha (TNF3), clone 52B83 82 MON4030B TLR2, clone TL2.1, Biotinylated 89 MON4060 CD83, clone HB15e 53 MON4085 CD229, clone HLy9.1.25 68 MON5007 Immunoglobulins, clone MH14/1 107 MON4031 TLR2, clone TL2.3 90 MON4060B CD83, Biotinylated, clone HB15e 53 MON4085B CD229, Biotinylated, clone HLy9.1.25 68 MON5008 Immunoglobulins, clone MH25/1 107 MON4031B TLR2, clone TL2.3, Biotinylated 90 MON4060F CD83, FITC conjugated, clone HB15e 53 MON4085F CD229, FITC conjugated, clone HLy9.1.25 68 MON5009 Immunoglobulins, clone MH16/1 107 MON4032 TLR4/MD2, Mouse, clone MTS510 91 MON4060R CD83, R-PE conjugated, clone HB15e 53 MON4085R CD229, R-PE conjugated, clone HLy9.1.25 68 MON5010 Immunoglobulins, clone HB63 107 MON4032B TLR4/MD2, Mouse, clone MTS510, Biotinylated 91 MON4061 CD158b, clone GL183 64 MON4086 CD146, clone OJ79c 63 MON5011 Intestinal Alkaline Phosphatase,clone MIG-I15 83 MON4033 TLR4, clone HTA125 91 MON4062 CDw137, clone 4B4-1 62 MON4086F CD146, FITC conjugated, clone OJ79c 63 MON5012 Follicle Stimulating Hormone, FSH, clone 1038 101 MON4033B TLR4, clone HTA125, Biotinylated 91 MON4062F CD137, FITC conjugated, clone 4B4-1 62 MON4087 CD180, clone MHR73 66 MON5013 Beta-Galactosidase from E. coli,clone BG-02 83 MON4034 Alpha-Fetoprotein (AFP), clone MBS-12 153 MON4062R CDw137, R-PE conjugated, clone 4B4-1 62 MON4087F CD180, FITC conjugated, clone MHR73 66 MON5014 IgG, clone EFE-565 107 MON4035 Alpha Fetoprotein (AFP), clone C2 153 MON4063 CD152, clone BNI3 64 MON4088 CD206, clone 15-2 67 MON5014F IgG, FITC conjugated, clone EFE-565 107 MON4036 Alpha Fetoprotein (AFP), clone C3 153 MON4064 CD227, clone C595(NCRC48) 68 MON4089 CD174, clone A70-C/C8 66 MON5014P IgG, Peroxidase conjugated, clone EFE-565 107 MON4037 Alpha Fetoprotein (AFP), clone D10 153 MON4064F CD227, FITC conjugated, clone C595(NCRC48) 68 MON4090 CD176, clone A78-G/A7 60 MON5015 IgM, clone ICL-931 109 MON4038 CD32, clone AT10 35 MON4065 CDw70, clone BU69 51 MON4091 CD195, clone HEK/1/85a 66 MON5015F IgM, FITC conjugated, clone ICL-931 109 MON4038B CD32, Biotinylated, clone AT10 35 MON4066 CD139, clone BU30 63 MON4091B CD195, Biotinylated, clone HEK/1/85a 66 MON5015P IgM, Peroxidase conjugated, clone ICL-931 109 MON4038F CD32, FITC conjugated, clone AT10 35 MON4067 CD49c, clone VM-2 44 MON4091F CD195, FITC conjugated, clone HEK/1/85a 66 MON5016 Transferrin, clone HTF-14 152 MON4039 CD62l, clone FMC46 49 MON4068 CD89, clone MIP8a 55 MON4091R CD195, R-PE conjugated, clone HEK/1/85a 66 MON5017 Interleukin 6, mouse, clone MP5-32C11 78 MON4039F CD62l, FITC conjugated, clone FMC46 49 MON4068F CD89, FITC conjugated, clone MIP8a 55 MON4092 CD230 (Aa109-112), clone 3F4 68 MON5018 Urokinase, clone PGM2001 84 MON4039R CD62l, R-PE conjugated, clone FMC46 49 MON4068R CD89, R-PE conjugated, clone MIP8a 55 MON4093 CD79 Beta, clone AT107-2 53 MON5019 Fibrin/Fibrinogen, clone MFB-HB 97 MON4040 Penicillin, clone Pen 9 150 MON4069 CD135, clone BV10A4H2 62 MON4093F CD79 Beta, FITC conjugated, clone AT107-2 53 MON5020 IL-2, clone F16 78 MON4041 CD86, clone BU63 54 MON4069B CD135, Biotinylated, clone BV10A4H2 62 MON4094 CD101, clone BB27 57 MON5021 C-Peptide, clone C-PEP-01 146 MON4041F CD86, FITC conjugated, clone BU63 54 MON4069F CD135, FITC conjugated, clone BV10A4H2 62 MON4094F CD101, FITC conjugated, clone BB27 57 MON5022 IL6, clone 5E1 78 MON4041R CD86, R-PE Conjugated, clone BU63 54 MON4069R CD135, R-PE conjugated, clone BV10A4H2 62 MON4094R CD101, R-PE conjugated, clone BB27 57 MON5022S IL-6, for ELISA, clone 5E1 78 MON4042 CD49a, clone TS2/7 43 MON4070 CD165, clone AD2-13H12 65 MON4095 CD150, clone A12 64 MON5023 Insulin, clone IN-05 103 MON4043 CD130, clone B-T2 62 MON4070F CD165, FITC conjugated, clone AD2-13H12 65 MON4095B CD150, Biotinylated, clone A12 64 MON5024 Albumin, clone A1-01 145 MON4044 CD102, clone B-T1 57 MON4070R CD165, R-PE conjugated, clone AD2-13H12 65 MON4095F CD150, FITC conjugated, clone A12 64 MON5025 LDL Apolipoprotein, Apo-B, clone5F8 159 MON4045 Proximal Nephrogenic Renal Antigen, clone PN-15 167 MON4071 CD112, clone R2-525 58 MON4096 CD94, clone DX22 55 MON5026 LDL Apolipoprotein, Apo-B, clone12G10 159 MON4046 Kinesin, clone KN-01 138 MON4072 CD122, clone MIK-beta 1 62 MON4096B CD94, Biotinylated, clone DX22 55 MON5027 LDL Apolipoprotein, Apo-B, clone4C11 159

194 www.monosan.com For research use only. Not for drug, diagnostic or other use. 195 Product Code Index Product Code Index

Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page MON5028 LDL Apolipoprotein, Apo-B, clone20 159 MON5056B IgG3, Biotinylated, Mouse, clone LO-MG3-7 108 MON6003 Tangles, clone MIG-T4 143 MON7002 Melanoma Discr. Antigen, clone PAL-M2 157 MON5029 LDL Apolipoprotein, Apo-E, clone3D12 159 MON5056F IgG3, FITC conjugated, Mouse, clone LO-MG3-7 108 MON6004 PCNA, clone PC10 16 MON7003 Melanoma Associated Antigen, clone NKI/C3 160 MON5030 HDL Apolipoprotein, Apo-AI, clone 1C5 158 MON5056P IgG3, Peroxidase conjugated, Mouse, clone LO-MG3-7 108 MON6005 Rat Endothelial Cell Antigen, clone HIS-52 167 MON7004 Melanoma Associated Antigen, clone NKI/M6 160 MON5032A Alpha-Fetoprotein, clone AFP-01 137, 153 MON5057 IgM, Mouse, clone LO-MM-9 109 MON6006 Serum Amyloid P, clone 5.4A 145 MON7005 Melanoma Associated Antigen, clone NKI/M7 160 MON5032B Alpha-Fetoprotein, clone AFP-11 137, 153 MON5057B IgM, Biotinylated, Mouse, clone LO-MM-9 109 MON6007 Serum Amyloid P, Biotinylated, clone 5.4D.3B 145 MON7006 Melanoma Associated Antigen, clone NKI/beteb 160 MON5033 Urokinase, clone PGM2005 84 MON5057F IgM, FITC conjugated, Mouse clone LO-MM-9 109 MON6008 Eosinophil Major Basic Protein, clone BMK-13 147 MON7007 Colon Carcinoma, clone CC-1 156 MON5034 IgG4, clone BL-G4/1 108 MON5058 Interferon Alpha 2B Recombinant, clone IFNA2.3 76 MON6009 Endothelial Internalizing Surface Antigen, GES-HB 157 MON7009 Small Cell Carcinoma, MOC-21 168 MON5035 IgE, clone BL-E9 107 MON5059 Beta-Glucuronidase, clone 105 146 MON6010 CD62-E, clone ENA1 5, 49 MON7010 Small Cell Carcinoma, cl.MOC-31 168 MON5037 Alpha-1-Antitrypsin, cloneF46.4.1 145 MON5060 Microtubule Associated Protein, clone MT01 128 MON6010B CD62-E, Biotinylated, clone ENA1 5, 49 MON7011 Small Cell Carcinoma, MOC-32 168 MON5038 Alpha-1-Antitrypsin-Z, clone F50.4.1 145 MON5062 HSP-60, clone LK-2 158 MON6011 CD62E, clone ENA2 49 MON7012 Small Cell Carcinoma, MOC-52 169 MON5039 Alpha-1-Antitrypsin NON-Z,clone F43.8.1 145 MON5063 HSP-60, clone LK-1 159 MON6013 Endothelial Differentiation Marker, clone 1F10 157 MON7013 Vascular endothelial cadherin, clone BV9 169 MON5041 Peroxidase Horse Radish, clone HP-3 83 MON5064 Fibronectin, clone Fn-4 97 MON6014 F VIII Related Antigen, KG7/30 158 MON7014 neu-oncogen protein, C-erbB-2, clone CB11 166 MON5042 Biotin, clone Bio-8 146 MON5065 Amyloid A, clone Reu-86.2 145 MON6016 CD105, Endoglin, clone PN-E2 58 MON7015 p53, clone BP53-12 166 MON5043 Streptavidin, clone Strep-10 152 MON5066 Human Ku Antigen, clone Ku15 149 MON6017 CD106, VCAM-1, clone 1G11B1 13, 58 MON7016 Colon Carcinoma, clone CC-3 156 MON5044 IgM, Chicken, IgG and IgA, clone 408-6.1 109 MON5067 IFN-alpha, clone F18 75 MON6017B CD106, VCAM-1, Biotinylated, clone 1G11B1 13, 58 MON7017 Melanoma Marker, clone 2G12 160 MON5045 IgG, Chicken, clone 409-3.1 107 MON5070 Dinitrophenyl Hapten, clone LO-DNP-2 146 MON6023 Liver FABP, clone L2B10 147 MON7019 CD21, C3d Receptor, clone 4C5 31, 155 MON5046 IgM, Chicken clone 408-5.1 109 MON5071 IFN-gamma, clone F1 77 MON6023B Liver FABP, clone L2B10, Biotinylated 147 MON7021 Ep-Cam (ESA), clone VU-1D9 6 MON5047 Ig Lambda-chain, clone ICO-16 109 MON5072 IFN-gamma, clone F3 77 MON6024 Liver FABP, clone K5A6 147 MON7022 E-Cadherin, Uvomorulin, L-CAM, clone 6F9 5 MON5048 IgY, Chicken, clone 7C2 109 MON5073 IL-6, clone MP5-20F3 78 MON6024B Liver FABP, clone K5A6, Biotinylated 147 MON7023 Macrophage Marker, clone 3A5 120 MON5048B IgY, Chicken, clone 7C2, Biotinylated 109 MON5074 IL-10, clone JES5-2A5 79 MON6029 NuMa, clone A73-B/D12 15 MON7024 E-Cadherin, Uvomorulin, L-Cam, clone 5H9 5 MON5048P IgY, Chicken, clone 7C2, Peroxidase conjugated 109 MON5075 IL-1 receptor (p80), clone Reg21 61, 87 MON6030 Double Stranded DNA, clone AE-2 15 MON7025 Tenascin, clone T2H5 152 MON5049 IL-8R1 (CXCR1), clone NVR1 62, 79 MON5076 IL-1 Receptor type 1 (p80), clone Reg20 61, 87 MON6035 Nuclear antigen 1, clone E1-2.5 126 MON7026 B-cells, clone MB2 116 MON5049B IL-8R1 (CXCR1), clone NVR1, Biotinylated 62 MON5077 IL-1 Receptor type 1 (p80), clone D1f3 61, 87 MON6040 MUC1, clone 126E7 161 MON7027 Extra Parotid Glycoprotein, clone 5E7 120, 158 MON5050 IgA, Mouse, clone LO-MA-7 107 MON5078 IL-1 Receptor type 1 (p80), clone D14e3 61, 87 MON6041 MUC1, clone 139H2 161 MON7029 Granzyme B, clone GrB-7 116 MON5050B IgA, Mouse, Biotinylated, clone LO-MA-7 107 MON5079 IL-1 RII, clone 8.5 61, 88 MON6042 MUC1, clone S1D12D7 161 MON7031 MCP-1, MCAF, clone MNA1 80 MON5050F IgA, FITC conjugated, Mouse, clone LO-MA-7 107 MON5080 IL-1 ra3, clone 2D11 88 MON6043 MUC1, clone VU-3C6 161 MON7033 GM-CSF, clone HP1-22E9 75 MON5051 IgE, mouse, clone LO-ME-3 107 MON5082 Gamma Interferon, clone F14 77 MON6044 MUC1, clone VU-3D1 162 MON7034 GM-CSF, clone MP1-13G6 75 MON5051B IgE, Biotinylated, Mouse, clone LO-ME-3 107 MON5083 Gamma Light Chain, Mouse, clone LO-MK-1 108 MON6045 MUC1, clone VU-4H5 162 MON7035 MCP-1, clone ECE.2 80 MON5051F IgE, FITC conjugated, Mouse, clone LO-ME-3 107 MON5083F Gamma Light Chain, FITC conj., Mouse, clone LO-MK-1 108 MON6046 MUC1, clone VU-11D1 162 MON7035B MCP-1, clone ECE.2, Biotinylated 80 MON5051P IgE, R-PE, Mouse, clone LO-ME-3 107 MON5084 IL-1 RII, CDw121b, clone 6G5 61, 88 MON6047 MUC1, clone VU-11E2 162 MON7036 IP-10, clone 6D4 80 MON5052 IgG, Mouse, clone LO-MG-7 108 MON5085 IFN-Alpha (II), clone N27 76 MON6048 MUC1, clone VU-12E1 163 MON7036B IP-10, clone 6D4, Biotinylated 80 MON5052B IgG, Biotinylated, Mouse, clone LO-MG-7 108 MON5086 Interferon Alpha 1, clone 2-48 75 MON6049 MUC1, clone VU-13F11 163 MON7037 P55,50, clone 1108-1 126 MON5052F IgG, FITC conjugated, Mouse clone LO-MG-7 108 MON5087 Interferon Alpha 1, clone 2-52 75 MON6050 MUC1, clone VU-2G7 163 MON7038 P105 (PANA), clone 2B3 16 MON5052P IgG, R-PE, Mouse, clone LO-MG-7 108 MON5088 Insulin, clone E2-E3 103 MON6051 MUC1, clone VU-4C2 163 MON7039 P34 cdc-2 Nuclear Protein, clonePOH-1 16 MON5053 IgG1, Mouse, clone LO-MG1-2 108 MON5089 IFN-Alpha (II), clone N39 76 MON6052 MUC1, clone VU-5F12 164 MON7041 TNF-Alpha, clone 4C6-H8 81 MON5053B IgG1, Mouse, Biotinylated, clone LO-MG1-2 108 MON5090 Microtubules, clone AE-8 128 MON6053 MUC2, clone Ccp58 164 MON7042 TNF-Alpha, clone J1D9 81 MON5053F IgG1, FITC conjugated, Mouse, clone LO-MG1-2 108 MON5091 Heat Shock Protein 27, clone G3.1 158 MON6054 MUC3, clone M3.1 164 MON7043 TNF-Alpha, clone J2D10 81 MON5053P IgG1, Peroxidase conjugated, Mouse, clone LO-MG1-2 108 MON5092 Interferon Alpha 2B Recombinant, clone IFNA2.4 76 MON6055 MUC5AC, clone 1-13M1 164 MON7044 Macrophages, clone LN-5 120 MON5054 IgG2a, Mouse, clone LO-MG2a-2 108 MON5093 Gamma Interferon, clone F12 77 MON6056 MUC5AC, clone 2-11M1 164 MON7045 Glycophorin, clone A63-B/C2 97 MON5054B IgG2a, Biotinylated, Mouse, clone LO-MG2a-2 108 MON5094 Insulin, clone 2D11-H5 103 MON6057 MUC5AC, clone 2-12M1 164 MON7046 Glycophorin, clone A84-B/H2 97 MON5054P IgG2a, R-PE, Mouse, clone LO-MG2a-2 108 MON5095 CD200r, clone OX108 67 MON6058 MUC5AC, clone 45M1 164 MON7047 Macrophage, clone EP-3 120 MON5055 IgG2b, Mouse, clone LO-MG2b-2 108 MON5095F CD200r, FITC conjugated, clone OX108 67 MON6059 MUC5AC, clone 58M1 165 MON7048 Human WAF1/p21 protein, clone WA-1 16 MON5055B IgG2b, Biotinylated, Mouse, clone LO-MG2b-2 108 MON5096 CD141, clone QBEND/40 63 MON6060 MUC5AC, clone 9-13M1 165 MON7049 p53, clone Pab122 166 MON5055F IgG2b, FITC conjugated, Mouse, clone LO-MG2b-2 108 MON5097 CD138, clone B-B4 63 MON6080 Hepatitis B virus, clone HB3 124 MON7050 Erythrocytes, clone SFL23.6 97 MON5055P IgG2b, Peroxidase conjugated, Mouse clone LO-MG2b-2 108 MON6001 Endothelium, clone PAL-E 157 MON6081 Hepatitis B virus, clone HB5 124 MON7051B CD61 Biotinylated, clone CLB-thromb/1 13, 48 MON5056 IgG3, Mouse, clone LO-MG3-7 108 MON6002 Endothelium, clone EN4 157 MON7001 Melanoma Discr. Antigen, clone PAL-M1 157 MON7051F CD61 FITC conjugated, clone CLB-thromb/1 13, 48

196 For research use only. Not for drug, diagnostic or other use. www.monosan.com 197 Product Code Index Product Code Index

Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page MON7060 Rabies Virus, clone RAB-50 126 MON9017 MRP1, clone MRPm6 129 MON9063 TNF-RII p75/p80, CD120b, clone MR37988 60, 94 PS048 Rabbit anti Collagen II 71 MON7061 Nuclear Membrane, clone AE-5 15 MON9018 MRP1, clone MRPr1 129 MON9063B TNF-Receptor p75, CD120b, Biotinylated, clone MR2-1 60, 94 PS049 Rabbit anti Collagen III 72 MON7070 FITC, clone F4/1 148 MON9019 MRP1, clone MRPm5 129 MON9064 TNF-RI (p55/p60), Mouse clone HM14 59, 93 PS050 Rabbit anti Collagen IV 73 MON7071 RBP (Human retinol Binding Protein), clone G4E4 151 MON9020 Annexin V, clone UMC01 146 MON9065 TNF-RII p75/p80, Mouse clone HM102 60, 94 PS051 Rabbit anti Collagen V 73 MON7080 Herpes simplex virus 1 and 2, clone T111 125 MON9021 Neural Cell Adhesion Molecule, NCAM, clone RNL-1 11 MON9067 TNF-Alpha, clone T3 81 PS052 Mouse TNF-alpha 80 MON7081 Herpes simplex virus type 1, clone T96 125 MON9026 MDR, cMOAT/MRP2, clone M2I-4 129 MON9067B TNF-Alpha, clone T3, Biotinylated 81 PS053 Rabbit x CD120a, TNF-RI (p55/p60) 59, 94 MON7082 Herpes simplex virus type 2, clone T303 125 MON9027 MRP2(ABCC2), clone M2III-6 130 MON9068 TNF-Alpha, clone T1 81 PS054 Rabbit x TNF-RII p75/p80, CD120b 60, 94 MON8001 Mouse-EGF, clone E5 86 MON9028 LRP/MVP, clone LMR5 133 MON9068B TNF-Alpha, clone T1, Biotinylated 81 PS055 Rabbit x Mouse CD120a TNF-RI p55/p60 60 MON8002 Mouse-EGF, clone F5 86 MON9029 Nitrotyrosine, clone HM11 141 MON9069 MRP4 (ABCC4), clone M4I-80 131 PS056 Rabbit x Mouse TNF-RII p75/p80, CD120b 60, 95 MON8003 Bromo-deoxy-Uridine, clone IIb5 146 MON9029B Nitrotyrosine, clone HM11, Biotinylated 141 MON9200 MDR Sampler Pack 134 PS057 Rabbit anti Collagen IV 72 MON8004 Chlamydia Trachomatis, clone 3 124 MON9030 MRP3(ABCC3), clone M3II-9 130 MON9501 Dendritic Cells, clone RFD1 116 PS058 Rabbit anti Chromogranin A+B 156 MON8006 Bromo-deoxy-Uridine/IdU, clone IU-4 127, 146 MON9031 MRP3, clone M3II-21 130 MSA1001 Streptavidin, FITC 171 PS059 Rabbit anti Rat Nitric oxide synthase 140 MON8009 Cytomegalovirus LA, clone BM219 124 MON9032 MRP2(ABCC2), clone M2II-12 130 MSA1004 Streptavidin, R-PE 171 PS060 Goat anti Collagen I 70 MON8014 Epidermal Growth Factor, clone F20 86 MON9033 MRP5(ABCC5), clone M5I-1 131 MSA1006 Streptavidin, TC 171 PS061 Goat anti Collagen II 71 MON8015 Intra-acrosomal protein,Mammalian, clone ACR.4 2 MON9034 MRP5(ABCC5), clone M5II-54 131 PA6003 Aluminium cryo tubes + caps, 3 ml 172 PS062 Goat anti Collagen III 71 MON8016 Intra-acrosomal protein, Mammalian, clone Hs-8 2 MON9035 MDR3 P-glycoprotein, clone P3II-26 129 PA6015 Aluminium cryo tubes + caps, 15 ml 172 PS063 Goat anti Collagen IV 72 MON8017 Intra-acrosomal protein, clone Hs-14 2 MON9036 LRP/MVP, clone MVP-37 133 PS006 Rabbit anti Rat IFN-gamma 77 PS064 Goat anti Collagen V 73 MON8019 Intra-acrosomal protein, Mammalian, clone Ds-2 2 MON9037 BCRP(ABCG2), clone BXP-34 132 PS007 Rabbit anti Human MCP-1 80 PS065 Rabbit anti Rat Collagen I 70 MON8034 Ribonucleoprotein Particles, clone 58-15 16 MON9038 P193/VPARP, clone P193-10 134 PS010 Rabbit anti Serotonin 142 PS066 Rabbit anti Rat Collagen III 72 MON8035 Leukemia cell line HL-60, clone IPO-M6 97, 159 MON9039 P193/VPARP, clone P193-4 134 PS011 Rabbit anti Human TNF-alpha 82 PS067 Rabbit anti Pig Collagen I and III 72 MON8039 HLA-B27, clone EP-4 116 MON9040 P193/VPARP, clone P193-6 134 PS014 Rabbit anti Mouse IL-6 79 PS068 Rabbit x Calf Collagen I and III 70 MON8040 Alkaline Phosphatase, clone V17.1 83 MON9041 BCRP(ABCG2), clone BXP-21 132 PS015 Rabbit anti Mouse IL-18 80 PS069 Rabbit anti Dog Collagen I and III 70 MON8050 Kappa Light Chain, clone L1C1 119 MON9042 Human MRP14, clone 47-8D3 121 PS016 Rabbit anti Human Interleukin-10 79 PS070 Rabbit anti LDL Apolipoprotein E, apo E 155 MON8054 GnRH receptor, clone A9E4 87 MON9043 Major Vault Protein, clone 1011 133 PS017 Rabbit anti E-Selectin, CD62-E (Biotinylated) 5, 49 PS071 Rabbit anti HDL Apolipoprotein AI,apo AI 154 MON8055 Golgi zone, clone AE-6 15 MON9044 MVP / LRP, clone 1014 133 PS018 Rabbit anti ICAM-1, CD54 (Biotinylated) 7, 46 PS072 Rabbit x HDL Apolipoprotein AI,apoAI 154 MON8056 GnRH Receptor, clone F1G4 87 MON9045 MVP/ LRP, clone 1027 133 PS019 Rabbit anti Human Interferon-Gamma 77 PS073 Rabbit anti Angiotensin I 99 MON8060 Thomsen-Friedenreich antigen, clone A78-G/A7 169 MON9046 MVP / LRP, clone 1032 134 PS021 Rabbit anti Rat Liver FABP 148 PS074 Rabbit anti Urokinase 84 MON8061 Thomsen-Friedenreich antigen, clone A68-B/A11 169 MON9047 MRP6(ABCC6), clone M6II-7 131 PS022 Rabbit anti Human Interstinal FABP 147 PS075 Goat anti Urokinase 85 MON9000 CD63, clone MEM-259 50 MON9048 MRP6(ABCC6), clone M6II-21 131 PS023 Rabbit anti Human Liver FABP 148 PS076 Rabbit anti alpha Fetoprotein AFP 154 MON9000F CD63 FITC conjugated, clone MEM-259 50 MON9049 MRP6(ABCC6), clone M6II-31 132 PS024 Rabbit anti Human Secretory Leucocyte Protease Inhibitor 151 PS077 Goat anti alpha Fetoprotein AFP 154 MON9002 Placental Alkine Phosphatase, clone MIG-P1 83 MON9050 BCRP(ABCG2), clone BXP-9 132 PS029 Rabbit anti Interleukin-1 RII 62, 88 PS079 Rabbit anti CGRP 100 MON9003 Ovarium Carcinoma Antigen, cloneOV632 166 MON9051 BCRP, clone BXP-53 132 PS030 Rabbit anti Human Tumor Necrosis Factor Alpha 82 PS080 Rabbit anti ACTH 99 MON9004 Neuro Specific Enolase, NSE, clone MIG-N3 140 MON9052 CD161, clone LT161 65 PS031 Rabbit anti Desmin 135 PS081 Rabbit anti Calcitonin 100 MON9005 Carcinoma Associated antigen MAM6, clone 115D8 156 MON9053 CD162, clone TB5 65 PS032 Rabbit anti GFAP 138 PS082 Rabbit anti LH-RH 104 MON9006 CD56, Neural Cell adh. Mol., NCAM, clone 123C3 10, 47 MON9054 CD13, clone WM15 27 PS033 Rabbit anti Keratin 110 PS083 Rabbit anti Somatostatin 105 MON9007 Renal Cell Carcinoma, clone RC38 167 MON9055 EPCR, clone LMR-42 96 PS036 Rabbit anti Human Growth Hormone 102 PS084 Rabbit anti p80NPM/ALK 122 MON9008 Carcinoembryonic Antigen, C.E.A., clone PARLAM4 155 MON9056 MRP2/cMOAT, clone M2III-5 130 ps037 Rabbit anti Human IL-6 79 PS085 Rabbit anti Gastrin 101 MON9009 Prostatic Specific Acid Phosphatase, PAP, clone 4L 84, 167 MON9057 MRP4 (ABCC4), clone M4I-10 131 ps038 Rabbit anti Human IL-8 79 PS086 Rabbit anti Glicentin 102 MON9010 Small Lung Cell Carcinoma, CD56,clone MOC-1 47 MON9058 CD120a, clone H398 59, 93 PS040 Rabbit anti Laminin 149 PS087 Rabbit anti Glucagon 102 MON9011 MDR1 P-gp (ABCB1), clone JSB-1 129 MON9058B CD120a, clone H398, Biotinylated 59, 93 PS041 Rabbit anti Collagen I 70 PS088 Rabbit anti Insulin 103 MON9011C Mouse IgG1 Negative Control Antibody, clone CT6 134 MON9059 CD120b, clone80M2 60, 95 PS042 Rabbit anti Collagen II 71 PS089 Rabbit anti Bombesin 99 MON9012 CD56, Neural Cell adh. Mol., NCAM, clone NKI-nbl-1 10, 47 MON9059B CD120b, clone80M2, Biotinylated 60 PS043 Rabbit anti Collagen III 71 PS090 Rabbit anti Neurotensin 140 MON9013 Synaptophysin, clone SY38 143 MON9060 TNF-alpha, Mouse, clone V1q 82 PS044 Rabbit anti Collagen IV 72 PS091 Rabbit anti Pancreas Polypeptide 104 MON9014 Chromogranin A, clone LK2H10 156 MON9061 TNF-Alpha, clone 4H31 81 PS045 Rabbit anti Collagen V 73 PS092 Rabbit anti Secretin 105 MON9015 Porcine Acrosin, clone ACR.2 2 MON9062 TNF-RI (p55/p60), CD120a, clone MR1-2 59, 94 PS046 Rabbit anti Collagen I,II,III,IV,V 71 PS093 Rabbit anti Substance P 142 MON9016 P110 glycoprotein, multidr.-res.rel. clone LRP-56 132 MON9062B TNF receptor, p55, clone MR1-2, Biotinylated 59, 94 PS047 Rabbit anti Collagen I 70 PS094 Rabbit anti Alpha-B-crystallin 137

198 www.monosan.com For research use only. Not for drug, diagnostic or other use. 199 Product Code Index Product Code Index

Cat. no. Description Page Cat. no. Description Page Cat. no. Description Page PS095 Sheep anti HDL Apolipoprotein AI, apoAI 154 PS114F Rabbit anti Horse IgG, IgM and IgA, FITC conjugated 170 PS244 Guinea-Pig anti Ornithine decarboxylase 83 PS096 Sheep anti HDL Apolipoprotein AI, apoAI 155 PS114P Rabbit anti Horse IgG, IgM and IgA, R-PE conjugated 170 PS246 Guinea-Pig anti VAChT 143 PS097 Murine MIP-1alpha 137 PS115 Rabbit anti Chicken IgY and IgA 170 PS247 Guinea-Pig anti VMAT2 143 PS100 Goat anti Human IgG 170 PS115F Rabbit anti Chicken IgY, FITC conjugated 170 PS248 Guinea-Pig anti Glucagon 102 PS100F Goat anti Human IgG, FITC conjugated 170 PS115P Rabbit anti Chicken IgY, R-PE conjugated 170 PS249 Guinea-Pig anti VIP 144 PS100P Goat anti Human IgG, Peroxidase conjugated 170 PS202 Rabbit anti-Enkephalin 101 PS250 Guinea-Pig anti Gastrin 101, 138 PS101 Goat anti Human IgM 170 PS203 Rabbit anti-alpha-MSH 104 PS251 Guinea-Pig anti Substance P 142 PS101F Goat anti Human IgM, FITC conjugated 170 PS204 Rabbit anti Somatostatin 106 PS252 Guinea-Pig anti CGRP 100 PS101P Goat anti Human IgM, Peroxidase conjugated 170 PS205 Rabbit anti Pancreastatin 105 PS253 Guinea-Pig anti PYY 150 PS102 Goat anti Human IgA 170 PS206 Rabbit anti Neuromedin U-8 140 PS254 Guinea-Pig anti CGRP 100 PS102F Goat anti Human IgA, FITC conjugated 170 PS207 Rabbit anti Oxytocin 104 PS255 Guinea Pig anti Adrenomedullin 99 PS102P Goat anti Human IgA, Peroxidase conjugated 170 PS208 Rabbit anti Motilin 104, 138 PS256 Guinea-Pig anti PNMT 150 PS103 Goat anti Human IgG, IgM and IgA 170 PS210 Rabbit anti Substance P 4-11 142 PS257 Rabbit anti NO-synthase 141 PS103F Goat anti Human IgG, IgM and IgA, FITC conjugated 170 PS211 Rabbit anti VAChT 143 PS258 Rabbit anti NO-synthase 141 PS103P Goat anti Human IgG, IgM and IgA, R-PE conjugated 170 PS212 Rabbit anti VAChT 143 PS259 Rabbit anti Ornithine decarboxylase 83, 142 PS104 Rabbit anti Human IgG 170 PS213 Rabbit anti VMAT2 143 PS260 Rabbit anti Histidine Decarboxylase 138 PS104F Rabbit anti Human IgG, FITC conjugated 170 PS214 Rabbit anti Amylin 99 PS261 Rabbit anti CEA 156 PS104P Rabbit anti Human IgG, Peroxidase conjugated 170 PS215 Rabbit anti Glucagon 102 PS262 Rabbit anti Chromogranin A+B 156 PS105 Rabbit anti Human IgM 170 PS216 Rabbit anti PP 105 PS263 Rabbit anti Rat CCK-A receptor 86 PS105F Rabbit anti Human IgM, FITC conjugated 170 PS217 Rabbit anti Secretin 105 PS264 Rabbit anti Capsaicin Receptor 86, 137 PS105P Rabbit anti Human IgM, Peroxidase conjugated 170 PS218 Rabbit anti VIP 144 PS265 Rabbit anti Rat NIS, Na+/I’ Symporter 166 PS106 Rabbit anti Human IgA 170 PS219 Rabbit anti GIP 148 PS276 Rabbit anti Lck-interacting molecule 18 PS106F Rabbit anti Human IgA, FITC conjugated 170 PS220 Rabbit anti Gastrin/CCK 102 PS277 Rabbit anti Lck-interacting molecule 18 PS106P Rabbit anti Human IgA, Peroxidase conjugated 170 PS221 Rabbit anti Glucagon/Glicentin 102 PS278 Rabbit anti SLP-76 19 PS107 Rabbit anti Human IgG, IgM and IgA 170 PS222 Guinea-Pig anti Insulin 103 PS279 Rabbit anti Csk-binding protein (PAG) 17 PS107F Rabbit anti Human IgG, IgM and IgA, FITC conjugated 170 PS223 Rabbit anti Bombesin/GRP 99 PS280 Rabbit anti BLNK (SLP-65) 17 PS107P Rabbit anti Human IgG, IgM and IgA, R-PE conjugated 170 PS224 Rabbit anti Neurotensin 140 T 10333 TUF Target Unmasking Fluid 172 PS108 Rabbit anti Goat IgG, IgM and IgA 170 PS225 Rabbit anti Substance P 142 T 10333 L TUF Target Unmasking Fluid 172 PS108F Rabbit anti Goat IgG, IgM and IgA, FITC conjugated 170 PS226 Rabbit anti CGRP 100 PS108P Rabbit anti Goat IgG, IgM and IgA, R-PE conjugated 170 PS227 Rabbit anti NPY 141 PS109 Rabbit anti Mouse IgG, IgM and IgA 170 PS228 Rabbit anti Galanin 137 PS109F Rabbit anti Mouse IgG, IgM and IgA, FITC conjugated 170 PS229 Rabbit anti PPY 150 PS109P Rabbit anti Mouse IgG, IgM and IgA, R-PE conjugated 170 PS230 Rabbit anti Prepro-NPY 68-97 (C-PON) 151 PS110 Rabbit anti Rat IgG, IgM and IgA 170 PS231 Rabbit anti NPY 1-20 141 PS110F Rabbit anti Rat IgG, IgM and IgA, FITC conjugated 170 PS232 Rabbit anti Neurokinin A/SubstanceK 139 PS110P Rabbit anti Rat IgG, IgM and IgA, R-PE conjugated 170 PS233 Rabbit anti Serotonin (5-HT) 142 PS111 Rabbit anti Guinea-Pig IgG, IgM and IgA 170 PS234 Rabbit anti PACAP 38 104 PS111F Rabbit anti Guinea-Pig IgG, IgM and IgA, FITC conjugated 170 PS235 Rabbit anti PACAP-related peptide 104 PS111P Rabbit anti Guinea-Pig IgG, IgM and IgA, R-PE conjugated 170 PS236 Rabbit anti GAP-43 137 PS112 Rabbit anti Sheep IgG, IgM and IgA 170 PS237 Rabbit anti Human Vesicular GABA Transporter 143 PS112F Rabbit anti Sheep IgG, IgM and IgA, FITC conjugated 170 PS238 Rabbit anti Helospectin 103 PS112P Rabbit anti Sheep IgG, IgM and IgA, R-PE conjugated 170 PS239 Rabbit anti CGRP 100 PS113 Rabbit anti Bovine IgG, IgM and IgA 170 PS240 Guinea-Pig anti Insulin/Proinsulin 104 PS113F Rabbit anti Bovine IgG, IgM and IgA, FITC conjugated 170 PS241 Rabbit anti Rat Pancreastatin 105 PS113P Rabbit anti Bovine IgG, IgM and IgA, R-PE conjugated 170 PS242 Rabbit anti Histamine 103 PS114 Rabbit anti Horse IgG, IgM and IgA 170 PS243 Guinea-Pig anti NO-synthase 141

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AUSTRALIA CANADA DENMARK GREECE

Jomar Diagnostics Cedarlane Laboratories Ltd. AH diagnostics EXELIXI S.A. P.O. Box 385 5516-8th Line RR#2 Runetoften 18 458 Messogion Str. Stepney SA 5069 Hornby, ON LOP 1EO DK-8210 AARHUS 153 42 ATHENS Tel. 1300 665 273 Tel. 800-268 5058 (toll-free) Tel. 8745 9010 Tel. 210-6010910 Fax. 08 8362 6388 Tel. 905 878 8891 Fax. 8745 1292 Fax. 210-6017503 E-mail: [email protected] Fax. 905 878 7800 E-mail: [email protected] E-mail: [email protected] Website: www.jomar.on.net E-mail: [email protected] Website: www.ahdiagnostics.dk Website: www.cedarlanelabs.com HONGKONG AUSTRIA FINLAND CHILE Jingmei Biotech co., ltd Szabo-Scandic Handels GmbH & Co KG Immuno Diagnostic Oy Prolab LTDA. 14Fl, Kaiyuan Plaza, No. 7001 Beihuan Ave Quellenstrasse 110 Kaivokatu 16 Shenzhen 518034 A-1100 Vienna Vergara # 24, Office 908 FIN-13100 HAMEENLINNA Tel. 0755 83546191 Tel. +43 1 4893861-0 Santiago Tel. 03 615 370 Fax. 0755 83546196 Fax. +43 1 4893961-7 Tel. 02 698 9617 Fax. 03 6822 039 E-mail: [email protected] E-mail: [email protected] Fax. 02 698 7215 E-mail: [email protected].fi Website: www.jingmei.com Website: www.szabo-scandic.com E-mail: [email protected] Website: www.immunodiagnostic.fi Website: www.prolab.cl HUNGARY BELGIUM FRANCE CHINA Frank Diagnosztika Kft. Sanbio b.v. Tebu - bio Jingmei Biotech co., ltd Dereglye u.2 Frontstraat 2a 39, Rue de Houdan H-1036 BUDAPEST 5405 PB UDEN 14Fl, Kaiyuan Plaza, No. 7001 Beihuan Ave BP15 Tel. 01 250 1813/01 388 3114 Tel. 02 2192 137 Shenzhen 518034 F-78612 LE PERRAY EN YVELINES CEDEX Fax. 01 368 5721 Fax. +31 413 266605 Tel. 0755 83546191 Tel. +33 1 30 46 39 00 E-mail: [email protected] E-mail: [email protected] Fax. 0755 83546196 Fax. +33 1 30 46 39 11 Website: www.frank-diagn.hu Website: www.sanbio.nl E-mail: [email protected] E-mail: [email protected] Website: www.jingmei.com Website: www.tebu-bio.com INDIA BRAZIL CZECH REPUBLIC GERMANY Biogenuix SG Tecnologia Clínica Ltda. BARIA s.r.o. Sanbio Deutschland GmbH 131-G.Floor; Rua Avanhandava, 675 A-6, Local Shopping Centre Sao Paulo - SP CEP 01306-001 Jizni 393 Vilshofener Strasse 35 Paschim Vihar Tel. 0055 11 3257-6811 252 44 Psary D-94501 BEUTELSBACH New Delhi-110063 E-mail: [email protected] Tel. +420 244 911 228 Tel. 08543 916182 Tel. +91-11-52321456,9810168857,9810029475 Website: www.sgtecnologia.com.br Fax. +420 244 911 228 Fax. 08543 91211 Fax. +91-25266937 Mobile +420 603 172 447 E-mail: [email protected] Email: [email protected] E-mail: [email protected] Website: www.sanbio.de Website: www.biogenuix.com Website: www.baria.cz

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IRELAND KOREA NETHERLANDS SINGAPORE

Bi-Med Ltd. Essence Medical Inc. Sanbio b.v. Innovative Biotech Pte Ltd 17, Milford 417, Leaders B/D, Yatapdong 342-1 Frontstraat 2a 67 Ayer Rajah Crescent Yellow Walls Rd. Bundanggu Seongnam 5405 PB UDEN # 07-10, Ayer Rajah Industrial Estate Malahide Gyeonggi-do / P.C. 463-828 Tel. +31 413 251115 SINGAPORE 139950 Co. Dublin Tel. 82 31 707 1544 Fax. +31 413 266605 Tel. 65-67791919 Tel. 00353 87 29 35 007 Fax. 82 31 707 1999 E-mail: [email protected] Fax. 65-67791112 Fax. 00353 84 55 901 E-mail: [email protected] Website: www.sanbio.nl E-mail: [email protected] E-mail: [email protected] Website: www.essencemedical.com Website: www.innobiotech.com.sg

NEW ZEALAND ISRAEL LUXEMBOURG SPAIN Pharmaco (NZ) Ltd. Enco Scientific Services Sanbio b.v. Deltaclon P.O. Box 4079 P.O.B. 2736 Frontstraat 2a Auckland Javier del Quinto no. 4 Petach-Tiqva 49127 5405 PB UDEN Tel. 09 377 3336 28043 MADRID Tel. 972-3-9349922 Tel. +31 413 251115 Fax. 09 307 1307 Tel. +34 91 764 5364 / 091 381 4275 Fax. 972-3-9349876 Fax. +31 413 266605 E-mail: [email protected] Fax. +34 91 381 4275 / 091 534 4958 E-mail: [email protected] E-mail: [email protected] E-mail: [email protected] Website: www.enco.co.il Website: www.sanbio.nl Website: www.deltaclon.com NORWAY ITALY MALAYSIA SWEDEN Sigma-Aldrich Norway A/S Valter Occhiena SRL Biotech Medical Corporation Sdn. Bhd Postboks 188, Leirdal Novakemi ab 1011 OSLO Via Rosta, 7 BIS No. 56, Jalan Bola Tampar 13/1 Tel. 231 76000 Getfotsv. 38A 10143 TORINO Seksyen 1340100 Fax. 231 76010 S-122 06 ENSKEDE (Stockholm) Tel. 011 771 6971 Shah Alam, Selangor E-mail: [email protected] Tel. 08 390490 Fax. 011 776 1800 Tel. 603-55115900 Website: www.sigma-aldrich.com/norway Fax. 08 6593512 E-mail: [email protected] Fax. 603-55116900 E-mail: [email protected] Website: www.valterocchiena.com E-mail: [email protected] Website: www.biotechmedical.com.my POLAND SWITSERLAND JAPAN MALTA MEDianus Biocoba AG Funakoshi Co., Ltd. Ul. Cystersow 13/2 EJ Busuttil Ltd 31-553 Krakow Christoph Merian-Ring 7 9-7 Hongo 2-Chome, Bunkyo Ku Tel. 0048126653131 CH-4153 REINACH BL1 113-0033 TOKYO Xorrox Road Fax. 0048126653132 Tel. +41 61 716 29 00 Tel. 03 5684 1620 B’Kara BKR 12 E-mail: [email protected] Fax. +41 61 712 11 17 Fax. 03 5684 1775 Tel. 0021 447184 Website: www.medianus.net E-mail: [email protected] E-mail: [email protected] Fax. 0021 445366 Website: www.biocoba.ch Website: www.funakoshi.co.jp E-mail: [email protected] Website: www.ejbusuttil.com

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TAIWAN

Taiwan Life Support Systems, Inc. 51F No 81, Sec. 2, Chen-Teh Road Taipei Tel. 866 (2) 2555-9700 Fax. 866 (2) 2555-9695 E-mail: [email protected] Website: www.tlss.com.tw

TURKEY

Dateks Teknik Sistemler Sanayi Ve Ticaret Ltd Sti Kazakistan Caddesi 141/2 06510 Bahcelievler ANKARA Tel. +90 312 212 70 97 Fax. +90 312 212 07 82 E-mail: [email protected] Website: www.dateks.com

UK

Caltag MedSystems Ltd Botyl Road Botolph Claydon Buckingham Bucks MK18 2LR Tel. 0800 279 9113 Fax. 0800 279 9114 E-mail: offi[email protected] Website: www.caltagmedsystems.co.uk

USA

Caltag Laboratories Inc. 1849 Bayshore blvd. BURLINGAME, CA94010 Tel. 800-874 4007 Fax. 650 652 9030 E-mail: [email protected] Website: www.caltag.com

Cell Sciences 480 Neponset St., Bldg. 12A Canton, MA 02021 Tel. 888-769 1246 or 781-828 0610 Fax. 781-828 0542 E-mail: [email protected] Website: www.cellsciences.com

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