Content Antibodies for research in the following areas: Page Acrosomal Proteins 2 Cell Adhesion Molecules 3 Cell Cycle Associated Markers 15 Cell Signaling Research 17 Cluster Differentiation Markers 21 Collagens 70 Cytokines 75 Enzymes 83 Growth Factors and Receptors 86 Haematology 96 Hormones 99 Immunoglobulins 107 Keratins 110 Lymphoid Markers 116 Microbiology/Virology 124 Microtubuli 128 Multidrug Resistance Antigens 129 Muscles 135 Neuroscience 137 Special Interest 145 Tumour Markers 153 Secondary Reagents 170 Mouse Isotype Controls 171 Streptavidin 171 Aluminium Cryo-tubes for Storage in Liquid Nitrogen 172 Target Unmasking Fluid 172 General Technical Instructions for Use 173 Product Name Index 176 Product Code Index 189 Distributor Information 202 F = Frozen Sections (IHC) IP = Immunoprecipitation P = Paraffin Sections (IHC) IF = Immunofluorescence C = Flow Cytometry EM = Electron Microscopy E = ELISA A = Agglutination WB = Western Blotting B = Blocking IC = Immuno Cytology N = Neutralization Inh = Inhibition of biological activity FS = Functional Studies MC = Micro Cytotoxity Testing All antibodies are reactive with human antigens, unless stated otherwise. Acrosomal Proteins Cell Adhesion Molecules Intra-acrosomal protein, Bull Intra-acrosomal protein αvβ3 INTEGRIN β Catenin Cat.no. MON 8015 (Monoclonal) Cat.no. MON 8017 (Monoclonal) Cat.no. MON 2032 (Monoclonal) Cat. no. MON 3055 (Monoclonal) NEW Clone ACR.4 Clone Hs-14 Clone BV3 Clone 9F2 The antibody reacts with intra-acrosomal protein 28 kDa of bull The antibody reacts with human intra-acrosomal proteins (220 kDa). This monoclonal antibody (subclass IgG1) binds to human αvβ3 integrin Beta-catenin belongs together with alpha- and gamma-catenin to the spermatozoa, can be used for detection of state of acrosome of Strong reactivity was also observed with mouse sperms. Monoclonal present on human endothelium cells. catenin family. Catenins mediates cell-cell adhesion by interaction with undamaged spermatozoa. antibodies can be used (in IF test) for diagnostic of sperm pathology cadherins. Beta-catenin is highly homologous to gamma-catenin (quantitative detection of proteins) and for evaluation of physiological Use: IP, C (plakoglobin) although its function differs from that of plakoglobin. Use: IC, WB state of sperm cells (state of acrosome before or after acrosome Quantity: 100 µg Whereas plakoglobin has been found to suppress tumorigenicity, Quantity: 100 µg reaction). Finally, monoclonal antibodies could be useful for selection beta-catenin potentiates hyperproliferation and tumor formation. In of suitable method of Fertilization (IUI, standard IVF, ICSI) in the the nucleus beta-catenin it complexes with transcription factors and laboratories of assisted reproduction. αvβ3 INTEGRIN thus regulates the expression of specific genes. By its dual role, i.e. a Intra-acrosomal protein, Canine structural role in cell-cell junctions and a regulatory role in the nucleus, Use: IC, WB beta-catenin can transduce changes in cell adhesion and junction Quantity: 100 µg Cat.no. MON 2033 (Monoclonal) formation to control transmembrane signalling and gene expression. Cat.no. MON 8019 (Monoclonal) Clone BV4 Use: WB, IP, IF Clone Ds-2 Acrosin, Porcine This monoclonal antibody (subclass IgG1) binds to human αvβ3 integrin Quantity: 100 µg This antibody reacts with several forms of canine intra-acrosomal present on human endothelium cells. proteins (92 and 42 kDa). Antibody can be used for detection of the state of acrosome of undamaged spermatozoa. Cat.no. MON 9015 (Monoclonal) Use: WB, IP, C β1 INTEGRIN Quantity: 100 µg Use: IC, WB Clone ACR.2 Quantity: 100 µg The antibody reacts with various forms of porcine acrosin (55, 53, 45 Cat.no. MON 2031 (Monoclonal) and 35 kDa) in the acrosome of undamaged spermatozoa. Negative α Catenin reactivity was observed with human, bull and canine sperms. Clone BV7 Intra-acrosomal protein This monoclonal antibody (subclass IgG1) binds to human β1-integrin Use: IC, WB Cat. no. MON 3083 (Monoclonal) NEW chain present on tumour cells. BV7 is active on HT-29 colon carcinoma Quantity: 100 µg cells and on HCCP-2998 tumour cells. BV7 binds to several other tumour Cat.no. MON 8016 (Monoclonal) Clone 1G5 cells (MG3 osteosarcoma, A375 melanoma, MHCC-1410 and Lovo The monoclonal antibody 1G5 reacts with human alpha-catenin. colon carcinoma) but does not affect adhesion to endothelial cells. Clone Hs-8 Alpha-catenin belongs together with beta- and gamma-catenin to the The antibody reacts with human and boar intra-acrosomal proteins catenin family. Catenins comprise a large family of Ca2+-dependent, Use: WB, IP, C, Inh. (220, 50, 34 and 21 kDa). Monoclonal antibodies can be used (in IF homotypic cell-cell adhesion molecules that play important roles in Quantity: 100 µg test) for diagnostic of sperm pathology (quantitative detection of development, epithelial cell polarity and tumor progression. Cadherins proteins) and for evaluation of physiological state of sperm cells (state are single pass, type I transmembrane proteins that are localized in of acrosome before or after acrosome reaction). Finally, monoclonal the adherens junction. To be fully functional, the cadherin cytoplasmic antibodies could be useful for selection of suitable method of domain must be linked to the actin cytoskeleton through a group of Fertilization (IUI, standard IVF, ICSI) in the laboratories of assisted proteins termed catenins: alpha-catenin, beta-catenin and plakoglobin. reproduction. Alpha catenin interacts with the cadherin indirectly via interactions with beta-catenin or plakoglobin. Use: IC, WB Quantity: 100 µg Use: WB, IP, IF Quantity: 100 µg 2 For research use only. Not for drug, diagnostic or other use. www.monosan.com 3 Cell Adhesion Molecules Cell Adhesion Molecules Barmotin/7H6 antigen Desmoglein 2 E-Cadherin, uvomorulin, L-CAM E-Selectin, CD62E, Biotinylated Cat. no. MON 3054 (Monoclonal) NEW Cat. no. MON 3057 (Monoclonal) NEW Cat.no. MON 7022 (Monoclonal) Cat.no. PS 017 (Polyclonal) Clone 7H6 Clone 6D8 Clone 6F9 The antibody reacts with the Lectin Domain of E-selectin, CD62-E, Tight junctions function to maintain cellular polarity and permeability Desmoglein 2 is a member of the desmosomal cadherin family that One of the epithelial cell adhesion molecules, E-Cadherin, plays an formerly designated Endothelial Leucocyte Adhesion Molecule-1 barriers in epithelial and endothelial cells. Several molecules of the tight comprise the desmogleins and desmocollins. Desmosomes are cell-cell important role in the formation of cell-cell contacts in epithelia (ELAM-1). The antibody reacts with human endothelial cells activated junction complex have been identified. The monoclonal antibody 7H6 junctions in most epithelial cells and serve to link the intermediate irrespective their origin form ecto-, meso- or endodermal tissue. This with TNF, IL-1 or endotoxin. The antibody was found to react also with reacts with a 155 kD tight junction-associated protein also designated filament cytoskeletons of adjacent cells. Within the desmosome the early adhesion event between epithelial cells is a prerequisite for the cells transfected with the ELAM-1 gene. The antibody inhibits the barmotin. This protein is preferentially localized at the periphery of tight extracellular domain of desmoglein is essential for calcium dependent assembly of all elements of the junctional complex. Furthermore, adhesion of granulocytes both neutrophilic and eosinophilic. junctions of hepatocytes and other epithelia. The 7H6 antigen is also heterophilic binding to the desmocollins, whereas the intracellular E-Cadherin plays a crucial role in the maintenance of the epithelial detectable in the intestine and kidney. Studies with Madin-Darby canine domain is essential for binding to the desmosomal plaque protein, junctional complex and is as such an important molecule in maintaining Use: F, IP, WB kidney (MDCK) cells indicate the 7H6 antigen to be an important plakoglobin. Desmoglein 2 is synthesized in the basal layers, depending epithelial integrity. Over 90% of the malignant tumors are carcinomas. Quantity: 100 µg molecule for regulation of the barrier function of tight junctions. on the certain types of epidermis. One of the prerequisites for the release of carcinoma cells from the Confocal laser scanning microscopy revealed that the expression of The antibody 6D8 reacts with the 165 kDa desmoglein 2 protein. primary site might be a defect in intercellular adhesion mediated by 7H6 antigen decreased sequentially during hepatocarcinogenesis. Furthermore it is not cross reactive with desmoglein 1 or desmoglein 3. the absence of E-Cadherin expression. Therefore, the expression of E-selectin, CD62E, ELAM-1 The 7H6 antigen was expressed intensely at the apical and basolateral E-Cadherin might be an important parameter for the determination of membrane of cancer cells with glandular pattern. Use: WB, IP, IF the invasive potential of epithelial neoplasms, and for the transition of Examination of human gastric and colon cancer tissues showed that Quantity: 100 µg a benign to a malignant neoplasm. Antigen used: human uvomorulin. Cat.no. MON 6010 (Monoclonal) tight junctions were maintained highly in the well-differentiated Antigen location: lateral border of epithelial cells. (gland-forming) adenocarcinomas, but reduced in poorly-differentiated Clone ENA1 adenocarcinomas. In MDCK cells the disappearance of 7H6 expression Desmoglein 3 Use: WB, F, P ENA1 reacts with E-selectin CD62-E, previous designated as the is closely related