Diptera, Mycetophilidae, Sciophilinae) from the Colombian High Andean Ecosystems, with Comments on the Genus
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ZooKeys 988: 129–150 (2020) A peer-reviewed open-access journal doi: 10.3897/zookeys.988.49627 RESEARCH ARTICLE https://zookeys.pensoft.net Launched to accelerate biodiversity research Four new Neotropical species of Eudicrana Loew (Diptera, Mycetophilidae, Sciophilinae) from the Colombian high Andean ecosystems, with comments on the genus Carolina Henao-Sepúlveda1, Marta Wolff1, Dalton de Souza Amorim2 1 Grupo de Entomología, Universidad de Antioquia, Calle 67 # 53–108, Medellín, Colombia 2 Departa- mento de Biologia, Faculdade de Filosofia, Ciências e Letras de Riberão Preto, Universidade de São Paulo, Avenida Bandeirantes 3900, 14040-901, São Paulo, Brazil Corresponding author: Carolina Henao-Sepúlveda ([email protected]) Academic editor: V. Blagoderov | Received 24 December 2019 | Accepted 20 October 2020 | Published 6 November 2020 http://zoobank.org/6361AF0D-919B-4876-92E7-0D1FFC911235 Citation: Henao-Sepúlveda C, Wolff M, Amorim DS (2020) Four new Neotropical species ofEudicrana Loew (Diptera, Mycetophilidae, Sciophilinae) from the Colombian high Andean ecosystems, with comments on the genus. ZooKeys 988: 129–150. https://doi.org/10.3897/zookeys.988.49627 Abstract Four new species of the sciophiline genus Eudicrana Loew are described for the Eastern and Central Andes of Colombia–Eudicrana silvaandina sp. nov., E. chingaza sp. nov., E. maculata sp. nov. and E. merizal- dei. These are the first species ofEudicrana described from the extreme northern range of the Andes. The altitudinal distribution of these species in the paramos and high Andean forest ecosystems is restricted to 1750–3660 m a.s.l. and some other information on the environment is briefly discussed. A key for the Colombian species of Eudicrana is provided and a discussion is elaborated on the position of these species within the genus. Keywords Andean ecosystem, biology, diversity, Neotropical region, taxonomy Copyright C. Henao-Sepúlveda et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 130 C. Henao-Sepúlveda et al. / ZooKeys 988: 129–150 (2020) Introduction The genus Eudicrana was originally proposed by Loew (1869) based on a single female of the type species of the E. obumbrata, from North America. The genus is also known from 11 other species: E. nigriceps Lundström from Europe (Lundström 1909) and E. affinis Okada from Japan (Okada 1938), E. monticola Tonnoir and E. nicholsoni Ton- noir from Australasian (Tonnoir 1929) and E. araucariae Matile, from New Caledonia (Matile 1991), and six Neotropical species–E. basinerva Freeman, E. pallida Freeman and E. similis Freeman, from the Argentinian Patagonia and southern Chile (Freeman 1951), E. claripennis Edwards and E. vittata Edwards, from Peru (Edwards 1931), and E. splendens Lane, from southeastern Brazil (Lane 1948). New combination– Neu- ratelia elegans (Lane, 1948)–was recently proposed for a Brazilian species Eudicrana elegans Lane by Henao-Sepúlveda et al. (2019). Eudicrana clearly belongs to the Sciophilinae, distinguished from other genera by the lack of the mid ocellus, the lateral ocelli touching the eye margins, and the presence of R4 forming an elongated rectangular cell (Borkent and Wheeler 2013). In Borkent and Wheeler’s (2013) phylogenetic study of the Sciophilinae, Eudicrana appears as monophyletic in a clade also including Polylepta Winnertz and its sister genus, Lepto- morphus Curtis. Both these genera share the presence of setae at the anterior part of the mediotergite. Analysis based on molecular data shows that Eudicrana is very close to Sciophila, but the studies are based on a very restricted taxon sampling of sciophiline genera (Ševčík et al. 2013; Kaspřák et al. 2018). Nearly nothing is known about its natural history. The genusEudicrana is one of the least studied genera of sciophilines. There has been no revision of the genus, despite the relatively low number of species, and the de- scriptions have very few illustrations. The Palearctic species, E. obumbrata has wing and terminalia illustrations (Lundström 1909: figs 155, 156; Johannsen 1910: figs 83,111; Fisher 1937: plate 11 fig. 1, Vockeroth 1981: fig.14.41).E. nigriceps, on the other hand, has only the terminalia illustrated by Hutson et al. (1980: fig. 188), who suggests that E. obumbrata and E. nigriceps may be synonymous due to slight differences between genitalia. In this case a slide montage or a new capture would be needed (Hutson 1979). Regarding the Australasian species, E. nicholsoni and E. monticola, the male terminalia are not illustrated; the last one has only the illustration of the wing by Tonnoir (1929: fig. 6), while Matile (1991: figs 7,8) illustrated the male terminalia and the wing ofE. araucariae. Of the Neotropical species, E. claripennis and E. vittata were described based only on females and have no illustrations at all. Lane (1960: fig. 8) assigned a specimen from Trinidad to E. vittata. The holotype of E. vittata has been examined, as well as the specimen studied by Lane (1960), and they may not be conspecific. Freeman (1951) illustrated the male terminalia of the Chilean species in lateral view and included a pho- tograph of the wing of E. basinerva, but important details of the terminalia are missing. Lane (1948) did not include an illustration of the wing of E. splendens. This paper describes the first four known species ofEudicrana of the high Andean ecosystems of the Central and Eastern Cordilleras of Colombia. This includes illustra- Four new Neotropical Andean Sciophilinae species of the genus Eudicrana 131 tions of the head, thorax, wing and male terminalia for all four species, and the female terminalia for one of the species. The paper also intends to discuss the similarities between the species of the genus. Material and methods The material studied here is deposited in the entomological collection Instituto de Investigación de Recursos Biológicos Alexander von Humboldt (IAvH-E) in Villa de Leyva, Boyacá, Colombia and the Colección Entomológica Universidad de Antioquia (CEUA), Medellin, Antioquia, Colombia. The specimens were collected in pristine ecosystems using Malaise trap and sweeping net (Fig. 1A–D), preserved in 70% and 96% ethanol. One wing of the holotypes and/ or paratypes was separated and mounted in permanent slide mountings with Euparal. The teminalia of the corresponding specimens were dissected and cleared in a solution of 10% KOH for 12 hours, then heated for 15 minutes, neutralized in acetic acid for 10 minutes, dehydrated in ethanol 70–96%, and preserved in a microvial in glycerine. Photographs were taken using a Moticam 3.0 megapixel digital camera attached to an Olympus SZX7 stereomicroscope and with a Leica DFC500 camera coupled to a Leica M16 stereomicroscope. Photo stacking was performed using the Helicon Focus 6.7.2 software and edited with Adobe Photoshop CC 2017. Photographs and illustra- tions of the terminalia were prepared using the U–DA Olympus drawing tube attached to an Olympus BX40 compound microscope, then vectorized with Illustrator CC 2017. Morphological terminology for head, thorax, pleural sclerites and terminalia fol- lows Søli (1997), while terminology of the wing venation follows Amorim and Rindal (2007) and Cumming and Wood (2017). Abbreviations ae = aedeagus; ce = cercus; gc = gonocoxite; gc ap = gono coxal apodeme; gc dl = gono- coxite dorso-apical lobe; gc dlp = gonocoxite dorso-lateral projection; gc vl = gono- coxite ventral lobe; gst = gonostylus; hyp = hypandrium; par = paremeres; par ap = parameral apodeme. Results Genus Eudicrana Loew Eudicrana Loew 1869: 142. Type species. Eudicrana obumbrata Leow (original designation). Diagnosis. (modified from Borkent and Wheeler 2013). Considerably large speci- mens, body elongate, mostly yellowish and/or brown. Two ocelli, in contact with eye 132 C. Henao-Sepúlveda et al. / ZooKeys 988: 129–150 (2020) Figure 1. A Landscape images of the municipality of San José de la Montaña, paramo El Congo, Co- lombia, type locality of Eudicrana maculata sp. nov. (holotype) B Malaise trap habitat is in area covered on A C landscape images of the municipality of Envigado, private property Nubarrones, type locality of E. merizaldei sp. nov. (holotype) D Malaise trap habitat is in area covered on C. margins. Laterotergite and mediotergite setose. Wing macrotrichia decumbent, some species without microtrichia. C clearly produced beyond apex of R5, sc-r reaching R1 almost always beyond origin of Rs, R4 present, forming an elongated rectangular cell, M1+2 short, barely twice length of r-m, M1+2 forking slightly more distally than origin of M4. Four new Neotropical Andean Sciophilinae species of the genus Eudicrana 133 Key to Colombian species of Eudicrana (males) 1 Wing membrane with obvious dark maculae (Fig. 4C, D); anepisternum with a set of dorsal short hairs (Fig. 3G, H) ................................................2 – Wing membrane clear or with faint darkened areas along anterior margin but no obvious maculae (Fig. 4A, B); anepisternum bare (Fig. 3E, F) ................3 2 Vein sc-r almost aligned with Rs (Fig. 4C); terminalia longer than wide and cercus almost twice the length of the gonocoxite.(Fig. 7D) ........................... ....................................................................................E. maculata sp. nov. – Vein sc-r not aligned with Rs (Fig. 4D); terminalia wider