Probiotic Delivery Through Non-Dairy Plant-Based Food Matrices
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agriculture Review Probiotic Delivery through Non-Dairy Plant-Based Food Matrices D. M. D. Rasika 1 , Janak K. Vidanarachchi 2, Selma F. Luiz 3, Denise Rosane Perdomo Azeredo 3, Adriano G. Cruz 3 and Chaminda Senaka Ranadheera 4,* 1 Department of Livestock & Avian Sciences, Faculty of Livestock Fisheries & Nutrition, Wayamba University of Sri Lanka, Makandura, Gonawila, Kuliyapitiya 60170, Sri Lanka; [email protected] 2 Department of Animal Science, Faculty of Agriculture, University of Peradeniya, Peradeniya, Kandy 20400, Sri Lanka; [email protected] 3 Department of Food, Federal Institute of Rio de Janeiro (IFRJ), CEP 20270-021 Rio de Janeiro, Brazil; [email protected] (S.F.L.); [email protected] (D.R.P.A.); [email protected] (A.G.C.) 4 Faculty of Veterinary & Agricultural Sciences, School of Agriculture & Food, The University of Melbourne, Melbourne, VIC 3010, Australia * Correspondence: [email protected]; Tel.: +61-3-9035-5836 Abstract: Probiotics are live microorganisms which, when administered in adequate amounts, confer a health benefit on the host. Traditionally, dairy products are the major and most popular probiotic carriers. At present, there is a growing demand for non-dairy probiotic products. Both fermented and non-fermented non-dairy plant-based food products are becoming highly appealing to both dairy and non-dairy consumers worldwide. Non-dairy plant-based food matrices such as fruits, vegetables, Citation: Rasika, D.M.D.; plant-based milk, cereals, and legumes have been used successfully in producing probiotic products Vidanarachchi, J.K.; Luiz, S.F.; with the minimum recommended viable probiotic numbers at the time of consumption. However, Azeredo, D.R.P.; Cruz, A.G.; due to the exclusion of dairy, whether these food matrices can enhance the functional properties Ranadheera, C.S. Probiotic Delivery of probiotics such as gastrointestinal survival and immune-enhancing effects needs a thorough through Non-Dairy Plant-Based Food investigation. Hence, this review focuses on some of the popular non-dairy plant-based probiotic Matrices. Agriculture 2021, 11, 599. food products and their microbiological quality characteristics in terms of maintaining probiotic https://doi.org/10.3390/ viability during product storage. Their gastrointestinal tolerance in these products, other functional agriculture11070599 properties, and product qualities have also been briefly discussed. Academic Editors: Karina Pierce and Keywords: probiotics; fruit and vegetable based probiotic products; soymilk; lactic acid bacteria; Isabel Lara Ayala Lactobacillus; Bifidobacterium; prebiotics Received: 14 May 2021 Accepted: 25 June 2021 Published: 28 June 2021 1. Introduction Publisher’s Note: MDPI stays neutral The utilization of beneficial microorganisms in health promotion has been practiced with regard to jurisdictional claims in in the means of fermented dairy products such as sour milk, yoghurt, and cheese from published maps and institutional affil- many thousands of years [1]. The idea that lactic acid bacteria (LAB) prevent intestinal iations. disorders and diseases is as old as the science of microbiology [2]. In 1907, the Nobel Laureate Elie Metchnikoff firstly documented the modern concept of probiotics in his book The Prolongation of Life after observing the Bulgarians’ exceptionally prolonged healthy living was due to their regular consumption of sour milk [3]. The first formula for the Copyright: © 2021 by the authors. deliberate administration of live LAB was a sour milk product based on a culture called Licensee MDPI, Basel, Switzerland. la Lactobacilline isolated by Metchnikoff [2]. The culture was comprised of Streptococcus This article is an open access article thermophilus and Lactobacillus delbrueckii subsp. bulgaricus and the product was launched distributed under the terms and in Paris at the beginning of the 20th century. Thereafter, many different of milk-based conditions of the Creative Commons probiotic products have been invented and introduced to the market. Probiotics can be Attribution (CC BY) license (https:// defined as live microorganisms which, in adequate amounts, confer a health benefit on creativecommons.org/licenses/by/ the host [4]. These organisms have to be taken regularly at sufficiently higher levels 4.0/). Agriculture 2021, 11, 599. https://doi.org/10.3390/agriculture11070599 https://www.mdpi.com/journal/agriculture Agriculture 2021, 11, x FOR PEER REVIEW 2 of 22 Agriculture 2021, 11, 599 2 of 23 (>10cfu/mL6 cfu/mL per day) per to day) avoidto avoidwashout washout and assure and assure that their that theirbenefits benefits will be will accrued be accrued in a insus- a sustainedtained manner manner [5]. [ 5 ]. LABLAB areare commoncommon membersmembers ofof thethe humanhuman gutgut microbiota,microbiota, whichwhich containscontains trillionstrillions ofof microorganismsmicroorganisms belongingbelonging to to more more than than 1000 1000 bacterial bacterial species. species. The The gut gut microbiota microbiota plays plays a significanta significant role role in thein the host host by influencingby influencin theg maturationthe maturation of the of immune the immune system, system, regulating regu- energylating energy metabolism, metabolism, and affecting and affecting brain functionbrain function and behaviour and behaviour through through the gut-brain the gut- axisbrain [3 axis,6,7]. [3,6,7]. Diet is Diet one is of one the mainof the factorsmain factors contributing contributing to the compositionto the composition and diversity and di- ofversity the human of the intestinalhuman intestinal microbiota. microbiota. Probiotics Probiotics and prebiotics and prebiotics have been have used been as dietaryused as strategiesdietary strategies aimed at aimed improving at improving host health host by he modulatingalth by modulating the gut ecosystemthe gut ecosystem which in which turn affectingin turn affecting host stress-responses, host stress-responses, behaviour, behaviour, and cognition and cognition [6]. [6]. ScientistsScientists selectselect potentialpotential probioticprobiotic strainsstrains basedbased onon theirtheir establishedestablished healthhealth benefits,benefits, howhow aa strainstrain cancan toleratetolerate thethe harshharsh gastrointestinalgastrointestinal tracttract conditions,conditions, safetysafety aspects,aspects, andand technologicaltechnological properties [7]. [7]. Some Some of of these these technological technological and and functi functionalonal properties properties are arede- depictedpicted in inFigure Figure 1.1 . FigureFigure 1.1. TechnologicalTechnological andand functionalfunctional propertiesproperties ofof aa potentialpotential probioticprobiotic strain. strain. LAB,LAB, mainly, Lactobacillus spp.spp. and and BifidobacteriumBifidobacterium spp.spp. are arewidely widely recognized recognized as pro- as probioticsbiotics since since they they commonly commonly possess possess probiotic probiotic characteristics characteristics [1,3]. Certain [1,3]. Certain strains strainsof pro- ofbiotics, probiotics, for example, for example, LactobacillusLactobacillus acidophilus acidophilus, Lactobacillus, Lactobacillus lactis, and lactis Pediococcus, and Pediococcus acidilactici, acidilacticihave been, havegiven been the Generally given the GenerallyRecognized Recognized as Safe (GRAS) as Safe status (GRAS) by the status U.S. by Food the U.S.and FoodDrug andAdministration Drug Administration (FDA) to (FDA)be used to as be food used ingredients as food ingredients [8]. Therefore, [8]. Therefore, these microor- these microorganismsganisms can be considered can be considered safe to consume safe to consume with very with little very or no little risk orto nothe riskhost. to Probiotics the host. Probioticscan be obtained can be from obtained different from sources different such sources as human such asgut human microbiota, gut microbiota, foods, and foods, other andnatural other environments. natural environments. They are Theyhost specific are host and, specific therefore, and, therefore, probiotic probiotic bacteria bacteriaisolated isolatedfrom human from intestinal human intestinalmicrobiota microbiota may be ad mayvantageous be advantageous for use in products for use inintended products for intendedhuman consumption for human [7]. consumption These probiotic [7]. Thesemicroorganisms probiotic microorganismshave various health-promoting have various health-promotingeffects, namely preventing effects, namely intestinal preventing tract infections, intestinal improving tract infections, lactose improving metabolism, lactose re- metabolism,ducing serum reducing cholesterol serum levels, cholesterol enhancing levels, immunity, enhancing stimulating immunity, stimulatingcalcium absorption, calcium absorption, improving protein digestibility, synthesizing vitamins (vitamin B including improving protein digestibility, synthesizing vitamins (vitamin B including nicotinic acid nicotinic acid and folic acid), and counteracting the effect of food-borne pathogens [9]. and folic acid), and counteracting the effect of food-borne pathogens [9]. The health ben- The health benefits of probiotic-enriched foods are expressed either directly through the efits of probiotic-enriched foods are expressed either directly through the interactions of Agriculture 2021, 11, 599 3 of 23 interactions of ingested live microorganisms with the host or indirectly as the result of the intake of microbial metabolites synthesized during fermentation [10]. Fermented dairy products