DOCSLIB.ORG

A Proteomic View of Probiotic Lactobacillus Rhamnosus GG

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
A Proteomic View of Probiotic Lactobacillus Rhamnosus GG View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Helsingin yliopiston digitaalinen arkisto Department of Veterinary Biosciences Faculty of Veterinary Medicine University of Helsinki Helsinki, Finland A proteomic view of probioƟ c Lactobacillus rhamnosus GG KerƩ u Koskenniemi ACADEMIC DISSERTATION To be presented, with the permission of the Faculty of Veterinary Medicine of the University of Helsinki, for public examinaƟ on in the Walter Auditorium, Agnes Sjöbergin katu 2, Helsinki, on January 13th 2012, at 12 o’clock noon. Helsinki 2012 Supervisor Docent Pekka Varmanen Division of Food Technology Department of Food and Environmental Sciences University of Helsinki Helsinki, Finland Reviewers Professor Sirpa Kärenlampi Department of Biosciences University of Eastern Finland Kuopio, Finland Docent Jaana Mättö Finnish Red Cross Blood Service Helsinki, Finland Opponent Professor Oscar Kuipers Department of Molecular Genetics Groningen Biomolecular Sciences and Biotechnology Institute University of Groningen Groningen, the Netherlands. Layout: Tinde Päivärinta Cover fi gure: A 2-D DIGE gel image (photo Kerttu Koskenniemi) ISBN 978-952-10-7403-5 (paperback) ISBN 978-952-10-7404-2 (PDF) ISSN 1799-7372 http://ethesis.helsinki.fi Unigrafi a Helsinki 2012 CONTENTS Abstract List of abbreviations List of original publications 1. Review of the literature ..........................................................................................1 1.1. Probiotic bacteria .....................................................................................................1 1.1.1. Common properties of probiotic bacteria .............................................. 1 1.1.1.1. Probiotic mechanisms .................................................................. 1 1.1.1.2. Main properties of probiotic bacteria .........................................2 1.1.1.3. Health-benefi cial eff ects of probiotics ........................................2 1.1.2. Lactobacilli ..................................................................................................3 1.1.2.1. Lactobacillus rhamnosus GG ........................................................3 1.1.3. Bifi dobacteria and propionibacteria ........................................................ 5 1.2. Proteomics ................................................................................................................. 5 1.2.1. Proteomic methods ........................................................................................5 1.3. Proteomics of potential probiotic bacteria ............................................................6 1.3.1. Basic proteome research ..........................................................................10 1.3.1.1. Proteome maps ............................................................................10 1.3.1.2. Comparison of growth phases ...................................................11 1.3.1.3. Comparison of diff erent strains and growth media ...............11 1.3.1.4. Development of proteomic methods ........................................12 1.3.2. Proteomics of stress responses of potentially probiotic bacteria ........13 1.3.2.1. Bile stress ......................................................................................13 1.3.2.2. Acid stress ....................................................................................22 1.3.2.3. Other stresses ...............................................................................23 1.3.3. Studies of the cell envelope proteomes and secretomes of potentially probiotic bacteria ..................................................................24 1.3.3.1. Cell envelope proteome ..............................................................24 1.3.3.2. Secretome .....................................................................................26 1.3.3.3. Surfome and secretome predictions .........................................27 1.3.4. Other proteomic studies of potentially probiotic bacteria .....................28 2. Aims of the study ..................................................................................................30 3. Materials and methods .........................................................................................31 3.1. Bacterial strain and growth conditions ...............................................................31 3.2. Proteomic methods ................................................................................................31 3.2.1. Protein extraction .....................................................................................31 3.2.2. Labeling of protein samples ....................................................................31 3.2.3. 2-D GE and image analysis .....................................................................32 3.3. Protein identifi cation by MS .................................................................................32 3.4. Transcriptomic methods .......................................................................................32 3.4.1. Experimental design, RNA methods, cDNA synthesis, and labeling 32 3.4.2. Scanning, image analysis, and data analyses .........................................33 3.5. Analysis of the phosphoproteome ........................................................................33 3.5.1. Protein extraction .....................................................................................33 3.5.2. 2-D GE and image analysis .....................................................................33 3.5.3. Phosphopeptide identifi cation ................................................................33 4. Results and discussion ..........................................................................................34 4.1. Eff ect of the growth medium on the proteome of L. rhamnosus GG ..............34 4.2. Growth phase-associated changes in L. rhamnosus GG ....................................35 4.3. Bile stress response of L. rhamnosus GG .............................................................37 4.4. Eff ect of growth pH on L. rhamnosus GG ...........................................................38 4.5. Protein phosphorylation in L. rhamnosus GG ....................................................39 4.6. Proteome map of L. rhamnosus GG .....................................................................39 5. Concluding remarks and future prospects ..........................................................43 6. Acknowledgements ...............................................................................................45 7. References .........................................................................................................46 Original publications ABSTRACT Lactobacillus rhamnosus GG is a probiotic bacterium that is known worldwide. Since its discovery in 1985, the health eff ects and biology of this health-promoting strain have been researched at an increasing rate. However, knowledge of the molecular biology responsible for these health eff ects is limited, even though research in this area has con- tinued to grow since the publication of the whole genome sequence of L. rhamnosus GG in 2009. Probiotic strains encounter various stress conditions during the production, product formulation, and passage through the gastro-intestinal tract (GIT), which may aff ect the functionality of these organisms. In this thesis, the molecular biology of L. rhamnosus GG was explored by mapping the changes in protein levels in response to diverse stress factors and environmental conditions. Th e proteomics data were supple- mented with transcriptome level mapping of gene expression. Th e harsh conditions of the GIT, which involve acidic conditions and detergent-like bile acids, are a notable challenge to the survival of probiotic bacteria. To simulate GIT condi- tions, L. rhamnosus GG was exposed to a sudden bile stress, and several stress response mechanisms were revealed. A noteworthy site of bile stress responses was the cell enve- lope, which was modifi ed in several ways to relieve the harmful eff ects of bile and to improve the adhesion properties of L. rhamnosus GG before the entrance to the gut, e.g., debasing of the negative charge of the cell envelope and reduction in the thickness of the exopolysaccharide layer. Mechanisms for recognizing bile compounds and actively removing them from the cell were activated by bile exposure, and several bile-induced changes in central metabolism were also detected. L. rhamnosus GG also responded in various ways to mild acid stress. Probiotic bacteria may face mild acid stress in dairy pro- duction because the pH is lowered by the production of acid by fermenting bacteria in fermented milk products. Th e acid stress response of L. rhamnosus GG included changes in central metabolism and in specifi c responses, such as the induction of proton-trans- locating ATPase, a membrane transporter used for increasing intracellular pH. Th ese results clearly showed that L. rhamnosus GG possesses a large repertoire of mechanisms for responding to stress conditions, which probably explains its good survival in GIT. Adaptation to diff erent growth conditions was studied by comparing the proteome lev- el responses of L. rhamnosus GG to diff erent growth media and to diff erent phases of growth. Th e growth phase-dependent changes were also mapped at the transcriptome level. Th e growth of L. rhamnosus GG in a rich laboratory medium, MRS (de Man – Rogosa – Sharpe),
Load more

Recommended publications
  • The Effect of Selected Herbal Extracts on Lactic Acid Bacteria Activity
    applied sciences Article The Effect of Selected Herbal Extracts on Lactic Acid Bacteria Activity Małgorzata Ziarno 1,* , Mariola Kozłowska 2 , Iwona Scibisz´ 3 , Mariusz Kowalczyk 4 , Sylwia Pawelec 4 , Anna Stochmal 4 and Bartłomiej Szleszy ´nski 5 1 Division of Milk Technology, Department of Food Technology and Assessment, Institute of Food Science, Warsaw University of Life Sciences–SGGW (WULS–SGGW), 02-787 Warsaw, Poland 2 Department of Chemistry, Institute of Food Science, Warsaw University of Life Sciences–SGGW (WULS–SGGW), 02-787 Warsaw, Poland; [email protected] 3 Division of Fruit, Vegetable and Cereal Technology, Department of Food Technology and Assessment, Institute of Food Science, Warsaw University of Life Sciences–SGGW (WULS–SGGW), 02-787 Warsaw, Poland; [email protected] 4 Department of Biochemistry and Crop Quality, Institute of Soil Science and Plant Cultivation, State Research Institute, 24-100 Puławy, Poland; [email protected] (M.K.); [email protected] (S.P.); [email protected] (A.S.) 5 Institute of Horticultural Sciences, Warsaw University of Life Sciences–SGGW (WULS–SGGW), 02-787 Warsaw, Poland; [email protected] * Correspondence: [email protected]; Tel.: +48-225-937-666 Abstract: This study aimed to investigate the effect of plant extracts (valerian Valeriana officinalis L., sage Salvia officinalis L., chamomile Matricaria chamomilla L., cistus Cistus L., linden blossom Tilia L., ribwort plantain Plantago lanceolata L., marshmallow Althaea L.) on the activity and growth of lactic acid bacteria (LAB) during the fermentation and passage of milk through a digestive system model. Citation: Ziarno, M.; Kozłowska, M.; The tested extracts were also characterized in terms of their content of polyphenolic compounds and Scibisz,´ I.; Kowalczyk, M.; Pawelec, S.; antioxidant activity.
    [Show full text]
  • Chronic Administration of Probiotic L. Rhamnosus Increases Anxiety-Like Behavior in Group-Housed Male Long Evans Rats
    University of Dayton eCommons Honors Theses University Honors Program 4-2018 Chronic Administration of Probiotic L. rhamnosus Increases Anxiety-like Behavior in Group-housed Male Long Evans Rats Parker Maddison Griff University of Dayton Follow this and additional works at: https://ecommons.udayton.edu/uhp_theses Part of the Biology Commons, and the Psychology Commons eCommons Citation Griff, Parker Maddison, "Chronic Administration of Probiotic L. rhamnosus Increases Anxiety-like Behavior in Group-housed Male Long Evans Rats" (2018). Honors Theses. 157. https://ecommons.udayton.edu/uhp_theses/157 This Honors Thesis is brought to you for free and open access by the University Honors Program at eCommons. It has been accepted for inclusion in Honors Theses by an authorized administrator of eCommons. For more information, please contact [email protected], [email protected]. Chronic Administration of Probiotic L. rhamnosus Increases Anxiety-like Behavior in Group-housed Male Long Evans Rats Honors Thesis Parker Maddison Griff Department: Psychology and Biology Advisor: Tracy Butler, Ph.D. and Yvonne Sun, Ph.D. April 2018 Chronic Administration of Probiotic L. rhamnosus Increases Anxiety-like Behavior in Group-housed Male Long Evans Rats Honors Thesis Parker Maddison Griff Department: Psychology and Biology Advisor: Tracy Butler, Ph.D. and Yvonne Sun, Ph.D. April 2018 Abstract Early life stress is a risk factor for later development of alcohol use disorders and anxiety disorders in humans. Using rodent experimental models, we know that rats experiencing social isolation as early-life stress exhibit greater anxiety-like behavior and alcohol consumption than rats housed in groups. Examining potential preventive strategies, we investigated the effects of probiotics, which have previously been shown to decrease rodent anxiety-like behavior, on the relationship between early-life stress and anxiety-like behavior in rats.
    [Show full text]
  • Antifungal Activity of Lactobacillus Pentosus ŁOCK 0979 in the Presence of Polyols and Galactosyl-Polyols
    Probiotics & Antimicro. Prot. https://doi.org/10.1007/s12602-017-9344-0 Antifungal Activity of Lactobacillus pentosus ŁOCK 0979 in the Presence of Polyols and Galactosyl-Polyols Lidia Lipińska1 & Robert Klewicki2 & Michał Sójka2 & Radosław Bonikowski3 & Dorota Żyżelewicz2 & Krzysztof Kołodziejczyk2 & Elżbieta Klewicka 1 # The Author(s) 2017. This article is an open access publication Abstract The antifungal activity of Lactobacillus pentosus Keywords Antifungal activity . Galactosyl-polyols . ŁOCK 0979 depends both on the culture medium and on the Lactobacillus . Metabolites . Polyols . SEM fungal species. In the control medium, the strain exhibited limited antagonistic activity against indicator food-borne molds and yeasts. However, the supplementation of the bac- Introduction terial culture medium with polyols (erythritol, lactitol, maltitol, mannitol, sorbitol, xylitol) or their galactosyl deriva- Filamentous fungi and yeasts are present in almost all types of tives (gal-erythritol, gal-sorbitol, gal-xylitol) enhanced the an- ecosystems due to their high adaptation ability and low nutri- tifungal properties of Lactobacillus pentosus ŁOCK 0979. Its tional requirements. Filamentous fungi are widespread food metabolites were identified and quantified by enzymatic spoilage microorganisms responsible for significant economic methods, HPLC, UHPLC-MS coupled with QuEChERS, losses in the agri-food industry [6]; they are also a major and GC-MS. The presence of polyols and gal-polyols signif- health concern due to mycotoxin production. The most com- icantly affected the acid metabolite profile of the bacterial mon genera of spoilage fungi include Penicillium, Fusarium, culture supernatant. In addition, lactitol and mannitol were Aspergillus, Cladosporium,andRhizopus [21]. Commercial used by bacteria as alternative carbon sources. A number of foodstuffs are usually protected from such microorganisms by compounds with potential antifungal properties were identi- physical and chemical techniques.
    [Show full text]
  • Promoting a Healthy Microbiome with Food and Probiotics
    PROMOTING A HEALTHY MICROBIOME WITH FOOD AND PROBIOTICS DEFINITIONS • Prebiotic: The food that probiotics need to sustain themselves (e.g. fructooligosaccharides) • Probiotic: A living organism that benefits the health of the host (e.g. bacteria and yeast) • Synbiotic: A supplement that contains both a prebiotic and a probiotic • Postbiotic: A metabolic byproduct of probiotics (e.g. n-butyrate from fermentation of fiber and bacteria) NUTRITION: THE ULTIMATE PREBIOTIC Although a person’s population of bacteria is inoculated at the time of birth, most of the microbiome is established in the human gut with the introduction of food. Breast milk includes milk oligosaccharides (MOS) that provide bacteria with nutrients to grow, particularly Bifidobacteria. Diets that contain the most fiber, fruit, and vegetables are known to engender the most diversity and richness of bacteria growth in the gut. Healthy bacteria produce short-chain fatty acids, such as n-butyrate, that support the health of the intestinal lining.[1] Foods rich in choline and carnitine (e.g. red meat and eggs) are metabolized by the intestinal microbiota to form the gas trimethylamine (TMA). TMA is converted by the liver to trimethylamine oxide (TMAO). This substance (an unhealthy postbiotic) has been strongly linked to the development of atherosclerosis and coronary artery disease.[2] Eating a diet low in red meat and animal fat and rich in fibrous plants has been found to support bacteria in the gut that can reduce how much energy the body stores; our gut’s microbiome can influence one’s risk of obesity.[3] When humans were hunters and gatherers, it was harder to kill wild game, so eating meat and animal fat was less common.
    [Show full text]
  • Psychobiotics and the Gut–Brain Axis Open Access to Scientific and Medical Research DOI
    Journal name: Neuropsychiatric Disease and Treatment Article Designation: Review Year: 2015 Volume: 11 Neuropsychiatric Disease and Treatment Dovepress Running head verso: Zhou and Foster Running head recto: Psychobiotics and the gut–brain axis open access to scientific and medical research DOI: http://dx.doi.org/10.2147/NDT.S61997 Open Access Full Text Article REVIEW Psychobiotics and the gut–brain axis: in the pursuit of happiness Linghong Zhou1 Abstract: The human intestine houses an astounding number and species of microorganisms, Jane A Foster1,2 estimated at more than 1014 gut microbiota and composed of over a thousand species. An indi- vidual’s profile of microbiota is continually influenced by a variety of factors including but 1Department of Psychiatry and Behavioural Neurosciences, McMaster not limited to genetics, age, sex, diet, and lifestyle. Although each person’s microbial profile is University, Hamilton, ON, Canada; distinct, the relative abundance and distribution of bacterial species is similar among healthy 2Brain-Body Institute, St Joseph’s Healthcare, Hamilton, ON, Canada individuals, aiding in the maintenance of one’s overall health. Consequently, the ability of gut microbiota to bidirectionally communicate with the brain, known as the gut–brain axis, in the modulation of human health is at the forefront of current research. At a basic level, the gut microbiota interacts with the human host in a mutualistic relationship – the host intestine pro- vides the bacteria with an environment to grow and the bacterium aids in governing homeostasis within the host. Therefore, it is reasonable to think that the lack of healthy gut microbiota may also lead to a deterioration of these relationships and ultimately disease.
    [Show full text]
  • Effect of Lactobacillus Rhamnosus HN001 in Pregnancy on Postpartum Symptoms of Depression and Anxiety: a Randomised Double-Blind Placebo-Controlled Trial
    EBioMedicine 24 (2017) 159–165 Contents lists available at ScienceDirect EBioMedicine journal homepage: www.ebiomedicine.com Research Paper Effect of Lactobacillus rhamnosus HN001 in Pregnancy on Postpartum Symptoms of Depression and Anxiety: A Randomised Double-blind Placebo-controlled Trial R.F. Slykerman a,F.Hoodb,K.Wickensb, J.M.D. Thompson a,C.Barthowb,R.Murphyc,J.Kangb, J. Rowden a, P. Stone d,J.Craneb, T. Stanley e,P.Abelsb,G.Purdief,R.Maudeg,E.A.Mitchella,⁎, the Probiotic in Pregnancy Study Group a Department of Paediatrics: Child and Youth Health, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand b Department of Medicine, University of Otago, P O Box 7343, Wellington, New Zealand c Department of Medicine, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand d Department of Obstetrics and Gynaecology, The University of Auckland, Private Bag 92019, Auckland 1142, New Zealand e Department of Paediatrics, University of Otago, P O Box 7343, Wellington, New Zealand f Dean's Office, University of Otago, P O Box 7343, Wellington, New Zealand g Graduate School of Nursing, Midwifery, and Health, Victoria University of Wellington, PO Box 600, Wellington 6140, New Zealand article info abstract Article history: Background: Probiotics may help to prevent symptoms of anxiety and depression through several putative mech- Received 2 July 2017 anisms. Received in revised form 31 August 2017 Objective: The aim of this study was to evaluate the effect of Lactobacillus rhamnosus HN001 (HN001) given in Accepted 13 September 2017 pregnancy and postpartum on symptoms of maternal depression and anxiety in the postpartum period.
    [Show full text]
  • Technological Characterisation of Probiotic Lactic Acid Bacteria As Starter Cultures for Dry Fermented Sausages
    foods Article Technological Characterisation of Probiotic Lactic Acid Bacteria as Starter Cultures for Dry Fermented Sausages 1 2,3,4 4 1, Nadia de L. Agüero , Laureano S. Frizzo , Arthur C. Ouwehand , Gonzalo Aleu y and Marcelo R. Rosmini 3,4,* 1 Faculty of Agricultural Sciences, Catholic University of Córdoba, 5000 Cordoba, Argentina; [email protected] (N.d.L.A.); [email protected] (G.A.) 2 Laboratory of Food Analysis “Rodolfo Oscar DALLA SANTINA”, Institute of Veterinary Science (ICiVet Litoral), National University of the Litoral—National Council of Scientific and Technical Research (UNL/CONICET), 3080 Esperanza, Argentina; [email protected] 3 Department of Public Health, Faculty of Veterinary Science, National University of Litoral, 3080 Esperanza, Argentina 4 Global Health and Nutrition Sciences, DuPont Nutrition and Biosciences, 02460 Kantvik, Finland; [email protected] * Correspondence: [email protected]; Tel.: +54-342-479-7991 Members of HealthyMeat-Network CYTED (ref. 119RT0568). y Received: 10 April 2020; Accepted: 1 May 2020; Published: 7 May 2020 Abstract: The objective of this study was to investigate probiotic microorganisms for use as starter cultures in dry fermented sausages production. A total of eight strains were studied evaluating technological and safety characteristics including the ability to grow, lactic acid production, gas formation, catalase activity, nitrate reductase activity, proteolytic activity, lipolytic activity, hydrogen peroxide production, salt tolerance, performance at
    [Show full text]
  • A Taxonomic Note on the Genus Lactobacillus
    TAXONOMIC DESCRIPTION Zheng et al., Int. J. Syst. Evol. Microbiol. DOI 10.1099/ijsem.0.004107 A taxonomic note on the genus Lactobacillus: Description of 23 novel genera, emended description of the genus Lactobacillus Beijerinck 1901, and union of Lactobacillaceae and Leuconostocaceae Jinshui Zheng1†, Stijn Wittouck2†, Elisa Salvetti3†, Charles M.A.P. Franz4, Hugh M.B. Harris5, Paola Mattarelli6, Paul W. O’Toole5, Bruno Pot7, Peter Vandamme8, Jens Walter9,10, Koichi Watanabe11,12, Sander Wuyts2, Giovanna E. Felis3,*,†, Michael G. Gänzle9,13,*,† and Sarah Lebeer2† Abstract The genus Lactobacillus comprises 261 species (at March 2020) that are extremely diverse at phenotypic, ecological and gen- otypic levels. This study evaluated the taxonomy of Lactobacillaceae and Leuconostocaceae on the basis of whole genome sequences. Parameters that were evaluated included core genome phylogeny, (conserved) pairwise average amino acid identity, clade- specific signature genes, physiological criteria and the ecology of the organisms. Based on this polyphasic approach, we propose reclassification of the genus Lactobacillus into 25 genera including the emended genus Lactobacillus, which includes host- adapted organisms that have been referred to as the Lactobacillus delbrueckii group, Paralactobacillus and 23 novel genera for which the names Holzapfelia, Amylolactobacillus, Bombilactobacillus, Companilactobacillus, Lapidilactobacillus, Agrilactobacil- lus, Schleiferilactobacillus, Loigolactobacilus, Lacticaseibacillus, Latilactobacillus, Dellaglioa,
    [Show full text]
  • Psychobiotics and the Manipulation of Bacteria–Gut–Brain Signals
    Review Psychobiotics and the Manipulation of Bacteria–Gut–Brain Signals 1 2,3 1 Amar Sarkar, Soili M. Lehto, Siobhán Harty, 4 5 6, Timothy G. Dinan, John F. Cryan, and Philip W.J. Burnet * Psychobiotics were previously defined as live bacteria (probiotics) which, when Trends ingested, confer mental health benefits through interactions with commensal Psychobiotics are beneficial bacteria fi gut bacteria. We expand this de nition to encompass prebiotics, which enhance (probiotics) or support for such bac- teria (prebiotics) that influence the growth of beneficial gut bacteria. We review probiotic and prebiotic effects bacteria–brain relationships. on emotional, cognitive, systemic, and neural variables relevant to health and – disease. We discuss gut brain signalling mechanisms enabling psychobiotic Psychobiotics exert anxiolytic and anti- depressant effects characterised by effects, such as metabolite production. Overall, knowledge of how the micro- changes in emotional, cognitive, sys- biome responds to exogenous influence remains limited. We tabulate several temic, and neural indices. Bacteria– important research questions and issues, exploration of which will generate brain communication channels through which psychobiotics exert effects both mechanistic insights and facilitate future psychobiotic development. We include the enteric nervous system suggest the definition of psychobiotics be expanded beyond probiotics and and the immune system. prebiotics to include other means of influencing the microbiome. Current unknowns include dose- responses and long-term effects. The Microbiome–Gut–Brain Axis The gut microbiome comprises all microorganisms and their genomes inhabiting the intestinal The definition of psychobiotics should tract. It is a key node in the bidirectional gut–brain axis (see Glossary) that develops through early be expanded to any exogenous influ- ence whose effect on the brain is colonisation and through which the brain and gut jointly maintain an organism's health.
    [Show full text]
  • The Effectiveness of Probiotic Lactobacillus Rhamnosus
    nutrients Article The Effectiveness of Probiotic Lactobacillus rhamnosus and Lactobacillus casei Strains in Children with Atopic Dermatitis and Cow’s Milk Protein Allergy: A Multicenter, Randomized, Double Blind, Placebo Controlled Study Bozena˙ Cukrowska 1,*, Aldona Ceregra 2, Elzbieta˙ Maciorkowska 3, Barbara Surowska 4, Maria Agnieszka Zegadło-Mylik 5, Ewa Konopka 1, Ilona Trojanowska 1, Magdalena Zakrzewska 3, Joanna Beata Bierła 1 , Mateusz Zakrzewski 3, Ewelina Kanarek 1 and Ilona Motyl 6 1 Department of Pathomorphology, the Children’s Memorial Health Institute, Aleja Dzieci Polskich 20, 04-730 Warsaw, Poland; [email protected] (E.K.); [email protected] (I.T.); [email protected] (J.B.B.); [email protected] (E.K.) 2 Outpatient Allergology and Dermatology Clinic, Patriotów St. 100, 04-844 Warsaw, Poland; [email protected] 3 Department of Developmental Age Medicine and Paediatric Nursing, Faculty of Health Sciences, Medical University of Bialystok, Szpitalna St. 37, 15-295 Białystok, Poland; [email protected] (E.M.); Citation: Cukrowska, B.; Ceregra, A.; [email protected] (M.Z.); [email protected] (M.Z.) 4 Outpatient Allergology Clinic, the Children’s Memorial Health Institute, Aleja Dzieci Polskich 20, Maciorkowska, E.; Surowska, B.; 04-730 Warsaw, Poland; [email protected] Zegadło-Mylik, M.A.; Konopka, E.; 5 Outpatient Dermatology Clinic, Chodakowska St. 8, 96-503 Sochaczew, Poland; [email protected] Trojanowska, I.; Zakrzewska, M.; 6 Department of Environmental Biotechnology, Lodz University of Technology, Wólcza´nska171/173, Bierła, J.B.; Zakrzewski, M.; et al. The 90-924 Łód´z,Poland; [email protected] Effectiveness of Probiotic Lactobacillus * Correspondence: [email protected]; Tel.: +48-22-815-19-69 rhamnosus and Lactobacillus casei Strains in Children with Atopic Abstract: Probiotics seem to have promising effects in the prevention and treatment of allergic Dermatitis and Cow’s Milk Protein conditions including atopic dermatitis (AD) and food allergy.
    [Show full text]
  • International Journal of Food Microbiology Influence of Passion
    International Journal of Food Microbiology 292 (2019) 126–136 Contents lists available at ScienceDirect International Journal of Food Microbiology journal homepage: www.elsevier.com/locate/ijfoodmicro Influence of passion fruit by-product and fructooligosaccharides onthe T viability of Streptococcus thermophilus TH-4 and Lactobacillus rhamnosus LGG in folate bio-enriched fermented soy products and their effect on probiotic survival and folate bio-accessibility under in vitro simulated gastrointestinal conditions Marcela Albuquerque Cavalcanti Albuquerquea,b, Debora Satie Yamacitaa, Raquel Bedania,b, ⁎ Jean Guy LeBlancc, Susana Marta Isay Saada,b, a Department of Biochemical and Pharmaceutical Technology, School of Pharmaceutical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 580, 05508-000 São Paulo, SP, Brazil b Food Research Center, University of São Paulo, São Paulo, SP, Brazil c CERELA-CONICET, C.P. T4000ILC San Miguel de Tucumán, Tucumán, Argentina ARTICLE INFO ABSTRACT Keywords: This study aimed to evaluate the influence of passion fruit by-product (PFBP) and fructooligosaccharides (FOS) Probiotic on the viability of Streptococcus thermophilus TH-4 and Lactobacillus rhamnosus LGG in folate bio-enriched fer- Folate mented soy products and their effect on probiotic survival and folate bio-accessibility under in vitro simulated Passion fruit by-product gastrointestinal conditions during storage of the products at 4 °C for up to 28 days (at days 1, 14, and 28). Kinetic Fructooligosaccharides parameters and folate contents before and after fermentation were also evaluated. Four different bio-enriched Fermented soy product soy products in which the two microorganisms were used in co-cultures were studied and PFBP and/or FOS were Bio-accessibility added at 1 g/100 g, except for the control product.
    [Show full text]
  • Psychobiotics; a Promise for Neurodevelopmental Therapy
    robioti f P cs o & l a H n e r a u l t o h J Daliri, J Prob Health 2016, 4:2 Journal of Probiotics & Health DOI: 10.4172/2329-8901.1000146 ISSN: 2329-8901 Review Article Open Access Psychobiotics; A Promise for Neurodevelopmental Therapy Eric Banan-Mwine Daliri1, Deog H Oh1 and Byong H Lee2* 1Department of Food Science and Biotechnology, Kangwon National University, Chuncheon, South Korea 2Department of Microbiology/Immunology, McGill University, Montreal, QC, Canada *Corresponding author: Byong Lee, Department of Microbiology/Immunology, McGill University, Montreal, QC, Canada, Tel: +821047799808; E-mail: [email protected] Received date: Feb 10, 2016; Accepted date: Apr 04, 2016; Published date: Apr 11, 2016 Copyright: © 2016 Daliri EBM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Abstract The inseparable association between man and microbes has long been known and some of their benefits are well documented. However, the use of bacteria as therapeutics has attracted much interest. Much is known about the ability of gut microbes to enhance immunity, lower cholesterol levels, improve gut barrier functions and many more benefits. Although it is evident that the gut and brain communicate through biochemical signaling which often involves the gut microbiota, it is still not clear whether or not gut manipulations through probiotic administration can correct or treat neurological problems. Many studies have shown that patients with neurodegenerative diseases also have gut dysbiosis and other studies have also shown the ability of certain gut bacteria to synthesize neurotransmitters.
    [Show full text]