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Microbiology Laboratory Exercises Third Edition 2020
MICROBIOLOGY Laboratory Exercises Third Edition Keddis & Rauschenbach 2020 Photo Credits (in order of contribution): Diane Davis, Ines Rauschenbach & Ramaydalis Keddis Acknowledgements: Many thanks to those in the Department of Biochemistry and Microbiology, Rutgers University, who have through the years inspired our enthusiasm for the science and teaching of microbiology, with special thanks to Diane Davis, Douglas Eveleigh and Max Häggblom. Safety: The experiments included in this manual have been deemed safe by the authors when all necessary safety precautions are met. The authors recommend maintaining biosafety level 2 in the laboratory setting and using risk level 1 organisms for all exercises. License: This work is licensed under a Creative Commons Attribution- NonCommercial-NoDerivatives 4.0 International License Microbiology Laboratory Exercises Third Edition 2020 Ramaydalis Keddis, Ph.D. Ines Rauschenbach, Ph.D. Department of Biochemistry and Microbiology Rutgers, The State University of New Jersey CONTENTS PAGE Introduction Schedule ii Best Laboratory Practices Iii Working in a Microbiology Laboratory iv Exercises Preparation of a Culture Medium 1 Culturing and Handling Microorganisms 3 Isolation of a Pure Culture 5 Counting Bacterial Populations 8 Controlling Microorganisms 10 Disinfectants 10 Antimicrobial Agents: Susceptibility Testing 12 Hand Washing 14 The Lethal Effects of Ultraviolet Light 15 Selection of Fungi from Air 17 Microscopy 21 Morphology and Staining of Bacteria 26 Microbial Metabolism 30 Enzyme Assay 32 Metabolic -
Rapid Identification Ofbacteroides Fragilis with Bile and Antibiotic Disks D
JOURNAL OF CuNICAL MICROBIOLOGY, Apr. 1977, p. 439-443 Vol. 5, No. 4 Copyright C 1977 American Society for Microbiology Printed in U.S.A. Rapid Identification ofBacteroides fragilis with Bile and Antibiotic Disks D. L. DRAPER1 AND A. L. BARRY* Section ofInfectious and Immunologic Diseases, School of Medicine, University of California, Davis, California 95616, and Clinical Microbiology Laboratories, Medical Center, Sacramento, California 95817* Received for publication 21 December 1976 A simple screening test is described for separating Bacteroides fragilis from other anaerobic gram-negative bacilli. The test utilizes filter paper disks im- pregnated with 25 mg of oxgall (Difco), tested in conjunction with antibiotic identification disks. The bile disks and antibiotic disks are placed on a supple- mented brucella blood agar plate which has been inoculated by swabbing with a standardized cell suspension. After 24 h at 350C in a GasPak jar, resistance to kanamycin and bile is taken as a presumptive identification of B. fragilis. Susceptibility to one or both disks indicates the need for further identification and additional biochemical tests are required. Those strains that produce insuf- ficient growth within 24 h are not likely to be B. fragilis. The reliability of the bile disk method was tested by comparing results with 100 clinical isolates versus results with bile in thioglycolate broth, peptone-yeast extract-glucose broth, and tryptic soy agar. All four bile test methods gave equilvalent results, but the broth media required much longer periods of incubation. Bacteroides fragilis is the anaerobic gram- would quickly identify most B. fragilis strains negative bacillus most frequently recovered could significantly reduce the number of iso- from human infections. -
Get Consistent and Reliable Results
Need help selecting? M Microbiology Free Tech Support 800-323-4340 Cole-Parmer® Dehydrated Culture Media Get consistent and reliable results – Use when ready or dehydrated culture extends shelf life – Individually numbered with certificate of compliance expiration date, and shelf-life guarantee – Quick dissolve for faster processing – For laboratory use only Media, Dehydrated Media, Media Description Size (g) Catalog number Price Agar, bacteriological This clear gel agar is high grade and manufactured using the ice method 500 GH-14200-10 Azide dextrose broth Use for detection of fecal Streptococci in water and sewage 500 GH-14202-00 Baird parker agar Use for the selective isolation of coagulase positive Staphylococci in food 500 GH-14202-01 Blood agar base with low pH Use to cultivate a wide variety of bacteria 500 GH-14202-02 This is used as a differential medium in the isolation and presumptive identification of Bile Esculin agar 500 GH-14202-03 enterococci/group D streptococci Brain heart infusion agar Use to cultivate a wide spectrum of bacteria, yeasts, and molds 500 GH-14200-12 Brain heart infusion broth Use to cultivate fastidious aerobic and anaerobic bacteria 500 GH-14202-05 Brilliant green bile broth A standard methods medium for confirmed and completed tests for coliforms in water 500 GH-14200-14 Buffered peptone water Helps in the recovery of Salmonella from foods after preservation techniques 500 GH-14202-08 Cetrimide agar Use for the selective isolation of Pseudomonas aeruginosa 500 GH-14200-52 This broth is especially suited for environmental sampling where neutralization of the chemical D/E neutralizing Broth 500 GH-14202-11 is important to determining the bactericidal activity Use to isolate E. -
Medical Bacteriology
LECTURE NOTES Degree and Diploma Programs For Environmental Health Students Medical Bacteriology Abilo Tadesse, Meseret Alem University of Gondar In collaboration with the Ethiopia Public Health Training Initiative, The Carter Center, the Ethiopia Ministry of Health, and the Ethiopia Ministry of Education September 2006 Funded under USAID Cooperative Agreement No. 663-A-00-00-0358-00. Produced in collaboration with the Ethiopia Public Health Training Initiative, The Carter Center, the Ethiopia Ministry of Health, and the Ethiopia Ministry of Education. Important Guidelines for Printing and Photocopying Limited permission is granted free of charge to print or photocopy all pages of this publication for educational, not-for-profit use by health care workers, students or faculty. All copies must retain all author credits and copyright notices included in the original document. Under no circumstances is it permissible to sell or distribute on a commercial basis, or to claim authorship of, copies of material reproduced from this publication. ©2006 by Abilo Tadesse, Meseret Alem All rights reserved. Except as expressly provided above, no part of this publication may be reproduced or transmitted in any form or by any means, electronic or mechanical, including photocopying, recording, or by any information storage and retrieval system, without written permission of the author or authors. This material is intended for educational use only by practicing health care workers or students and faculty in a health care field. PREFACE Text book on Medical Bacteriology for Medical Laboratory Technology students are not available as need, so this lecture note will alleviate the acute shortage of text books and reference materials on medical bacteriology. -
Obligate Anaerobe in Thioglycollate Medium
Obligate Anaerobe In Thioglycollate Medium Restiform and pernicious Monty addled her libertarians chastens rurally or alluding drawlingly, is Lorne ungainful? Ernst often laicises intrusively when haustellate Talbert underpeep parchedly and skiagraph her therapies. Adair uncanonizing shily as Mozartean Gifford tellurizing her unsociableness disentwining septically. Anaerobic bacteria can be identified by separate them in test tubes of thioglycollate broth. Aerobic and Anaerobic Growth Lab Exercise 6 Sp16 Canvas. Enriched Thioglycollate Broth is recommended for the isolation cultivation and identification of society wide following of obligate anaerobic bacteria Composition. How to the center of oxygen allowing the pus should not as they may live session is medium in thioglycollate are entire plate in air and aerobic respiration. We extend such an organism an aerotolerant anaerobe and set just off against an additional category of oxygen relationship Those left black the facultative. A movie free from thioglycollate for the growth of aerobic and anaerobic. Opened under a medium onto a medium on medium low, obligate anaerobe in thioglycollate medium, obligate and environmental parameters. Anaerobic Thioglycollate Medium Base Himedia. Find each pipette, obligate anaerobe growth. Incubation obligate anaerobes will grow abnormal in that portion of turkey broth for the. Obligate Anaerobes are actually poisoned by various forms of oxygen. Enriched Thioglycollate Broth M73 VWR. BBL Thioglycollate Medium Enriched with CiteSeerX. The model organism Escherichia coli is a facultative anaerobic bacterium ie it is solar to operate in both aerobic and anaerobic environments. Thioglycollate medium Difco containing 1 dextrose and B. Oxygen Requirements for Microbial Growth Microbiology. Fluid Thioglycollate Medium Flashcards Quizlet. The Thioglycolate medium other the word hand stands out fast its ability to. -
Isolation of Rhizobacteria in Southwestern Québec, Canada: An
Isolation of rhizobacteria in Southwestern Québec, Canada: An investigation of their impact on the growth and salinity stress alleviation in Arabidopsis thaliana and crop plants Di Fan Department of Plant Science Faculty of Agricultural and Environmental Sciences Macdonald Campus of McGill University 21111 Lakeshore Road, Sainte-Anne-de-Bellevue, Québec H9X 3V9 December 2017 A thesis submitted to McGill University in partial fulfillment of the requirements of the degree of DOCTOR OF PHILOSOPHY © Di Fan, Canada, 2017 Table of contents Abstract ................................................................................................. x Résumé ................................................................................................ xiii Acknowledegments ............................................................................. xv Preface ................................................................................................ xviii Contribution of authors ................................................................................ xviii Chapter 1................................................................................................. 1 Introduction ............................................................................................ 1 Chapter 2................................................................................................. 5 Literature Review ................................................................................... 5 2.1 What are root exudates? ......................................................................... -
BD Industry Catalog
PRODUCT CATALOG INDUSTRIAL MICROBIOLOGY BD Diagnostics Diagnostic Systems Table of Contents Table of Contents 1. Dehydrated Culture Media and Ingredients 5. Stains & Reagents 1.1 Dehydrated Culture Media and Ingredients .................................................................3 5.1 Gram Stains (Kits) ......................................................................................................75 1.1.1 Dehydrated Culture Media ......................................................................................... 3 5.2 Stains and Indicators ..................................................................................................75 5 1.1.2 Additives ...................................................................................................................31 5.3. Reagents and Enzymes ..............................................................................................75 1.2 Media and Ingredients ...............................................................................................34 1 6. Identification and Quality Control Products 1.2.1 Enrichments and Enzymes .........................................................................................34 6.1 BBL™ Crystal™ Identification Systems ..........................................................................79 1.2.2 Meat Peptones and Media ........................................................................................35 6.2 BBL™ Dryslide™ ..........................................................................................................80 -
Protective Effects of Probiotics on Cognitive and Motor Functions, Anxiety Level, Visceral Sensitivity, Oxidative Stress And
life Article Protective Effects of Probiotics on Cognitive and Motor Functions, Anxiety Level, Visceral Sensitivity, Oxidative Stress and Microbiota in Mice with Antibiotic-Induced Dysbiosis Alisa Arslanova 1, Aksiniya Tarasova 1, Anastasia Alexandrova 2, Vera Novoselova 2, Ilnar Shaidullov 1 , Dilyara Khusnutdinova 3, Tatiana Grigoryeva 3, Dina Yarullina 2, Olga Yakovleva 1 and Guzel Sitdikova 1,* 1 Department of Human and Animal Physiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Republic of Tatarstan, Russia; [email protected] (A.A.); [email protected] (A.T.); [email protected] (I.S.); [email protected] (O.Y.) 2 Department of Microbiology, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Republic of Tatarstan, Russia; [email protected] (A.A.); [email protected] (V.N.); [email protected] (D.Y.) 3 “Omics Technologies” Laboratory, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420008 Kazan, Republic of Tatarstan, Russia; [email protected] (D.K.); [email protected] (T.G.) * Correspondence: [email protected] Citation: Arslanova, A.; Tarasova, A.; Abstract: Accumulating clinical and preclinical data indicate a prominent role of gut microbiota in Alexandrova, A.; Novoselova, V.; regulation of physiological functions. The gut-brain axis imbalance due to gut dysbiosis is associated Shaidullov, I.; Khusnutdinova, D.; with a range of neurodegenerative diseases. Probiotics were suggested not only to restore intestinal Grigoryeva, T.; Yarullina, D.; dysbiosis but also modulate stress response and improve mood and anxiety symptoms. In this study, Yakovleva, O.; Sitdikova, G. we assessed the effects of probiotic lactobacilli on behavioral reactions, the level of oxidative stress Protective Effects of Probiotics on and microbiota content in mice administered to broad-spectrum antibiotics. -
Differences in the Composition of Cultivable Aerobic and Facultative Anaerobic Oral Microbiota in Cats of Various Age Groups
DOI: 10.2478/fv-2021-0009 FOLIA VETERINARIA, 65, 1: 67—74, 2021 DIFFERENCES IN THE COMPOSITION OF CULTIVABLE AEROBIC AND FACULTATIVE ANAEROBIC ORAL MICROBIOTA IN CATS OF VARIOUS AGE GROUPS Sondorová, M., Koščová, J., Kačírová, J., Maďar, M. Department of Microbiology and Immunology, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 041 81 Košice Slovakia [email protected] ABSTRACT oral micro biota were examined, differences between age groups were noted. The microbial diversity of the oral The feline oral cavity is naturally inhabited by var microbiota significantly increased with age. ious microorganisms contributing to the maintenance of its oral health. The imbalance of oral microbiota or Key words: age groups; cultivation methods; feline; the presence of pathogenic agents can lead to secondary oral microbiota oral diseases. Various factors such as sex, diet, breed, environment and even age, affect the composition of a healthy oral microbiota during the life of cats. The INTRODUCTION purpose of this study was to compare the composition of culturable aerobic and facultative anaerobic micro The oral cavity is the first part of the gastrointestinal biota in cats in terms of different age categories. We tract where the process of digestion begins, and therefore used conventional cultivation methods in conjunction it creates a space for the action of various microorganisms with microscopic and biochemical methods to isolate [8]. The constant flow of saliva and the unique biological and identify the micro organisms found in the oral cavi properties of each part of the oral cavity provide a place for ty of cats. The examination of 76 samples confirmed the attachment of microorganisms [22]. -
International Catalogue 2014
Microbiology Microbiology IVD IVD INTERNATIONALINTERNATIONAL CATACATALOGUELOGUE 2014 Microbiology In Vitro Diagnostics Viale Monza 272, 20128 Milan, Italy Biolife Leader in Microbiology 2 1 20162 0 6 CHROMOGENICCHHROOMOG6 CRE 1992 19929 C-EC AGARAGA [ \ 2 1 20152 0 5 of E.coli 5 CHROMOGENICCHHROOMOG ESBL for the detection of ESBL 1996 19961 1968 ALOA 19 19869 86 [ Dehydrated culture 6 medium for the media manufacturing. MUCAPMUCCAP TESTTE detection and [ enumeration of Hektoen Enteric Agar, L.monocytogenes Giolitti & Cantoni Broth, for Salmonella, made Staphylococci 110 Medium: Biolife 2 1 20132 0 3 [ 3 Biolife. CHROMOGENICCHHROOMOG STREP B for the detection of S.agalactiae 19 2 19 19821 1985 8 85 SELECTIVE MICROBIOLOGY8 MUG MEDIA 199898 19989 Biolife [ CHROMOGENICOMOGENIC Biolife SALMONELLA AGAR (CSA) [ culture media for the detection of 2 12 20122 0 Salmonella 0 CHROMOGENICCHHROOMOG CANDIDA AGAR for the detection of clinically important Candida! 2 2 20002 2006 000 006 IMMUNOCROMATHOGRAPHICIM0MUNOCR TESTS SENECA [ the enumeration of E.coli + Enterobacteriaceae Some of our milestones INTERNATIONAL CATALOGUE PRODUCTS FOR MICROBIOLOGY IN VITRO DIAGNOSTICS Biolife Italiana S.r.l. Mascia Brunelli S.p.A. International Catalogue - Finito di stampare nel mese di ottobre 2017 presso INGRAF srl (MI) - Rev. 11/2017 Biolife - Mascia Brunelli International Catalogue Biolife Italiana S.r.l. and Mascia Brunelli Spa are two sisters companies belonging to the same Group, operating, since long time, in the fields of Microbiology, Immunological IVDs and Medical Devices. Biolife Italiana S.r.l. has a well-established reputation in the production of powdered and ready-to use culture media for microbiology, supplements and enrichments, kits and reagents for microbial identifi- cation. -
CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (KV)
Difco & BBL Manual Manual of Microbiological Culture Media Second Edition Editors Mary Jo Zimbro, B.S., MT (ASCP) David A. Power, Ph.D. Sharon M. Miller, B.S., MT (ASCP) George E. Wilson, MBA, B.S., MT (ASCP) Julie A. Johnson, B.A. BD Diagnostics – Diagnostic Systems 7 Loveton Circle Sparks, MD 21152 Difco Manual Preface.ind 1 3/16/09 3:02:34 PM Table of Contents Contents Preface ...............................................................................................................................................................v About This Manual ...........................................................................................................................................vii History of BD Diagnostics .................................................................................................................................ix Section I: Monographs .......................................................................................................................................1 History of Microbiology and Culture Media ...................................................................................................3 Microorganism Growth Requirements .............................................................................................................4 Functional Types of Culture Media ..................................................................................................................5 Culture Media Ingredients – Agars ...................................................................................................................6 -
Microbiological Characterization and Sterilization-Induced Changes in the Profile of the Hydrophobic Organic Substances in Latvian Balneological Peat
Int. J. Environ. Sci. Technol. (2015) 12:2371–2380 DOI 10.1007/s13762-014-0638-4 ORIGINAL PAPER Microbiological characterization and sterilization-induced changes in the profile of the hydrophobic organic substances in Latvian balneological peat A. Ramata-Stunda • Z. Petrina • P. Mekss • G. Kizane • B. Silamikele • I. Muiznieks • V. Nikolajeva Received: 23 November 2013 / Revised: 5 May 2014 / Accepted: 9 June 2014 / Published online: 18 June 2014 Ó Islamic Azad University (IAU) 2014 Abstract The abundance and predominant groups of and ketones and decreased the amount of fatty acids. Heat bacteria, filamentous fungi and yeasts have been studied by sterilization proved to be more preserving for the peat culture-dependent microbiological methods in peat probes sterols than the radiation sterilization. It is concluded that obtained in two Latvian balneotherapy spa sites, Kemeri the heat and radiation sterilization methods induce different and Baldone. Unsterilized peat samples from both the sites changes of the profile of hydrophobic organic compounds contained 5.7–8.1 log bacterial colony-forming units of balneological peats, what may lead to different thera- (CFU) and 3.0–5.3 log fungal CFU per gram of dry peat. peutic effects at their application. Isolated species belonged to Alpha-, Beta-, and Gamma- Proteobacteria, Actinobacteria, Clostridia, Bacilli and Keywords Balneological peat Á Micro-organisms Á Flavobacteria as well as to filamentous fungi and yeasts. Gamma irradiation Á Electron beam irradiation Á Sterols The composition of microbial population of the peat from both sites shared just four micro-organism groups (Bacillus mycoides, Burkholderia cepacia, Streptomyces spp. and Introduction Trichoderma spp.) within totally 36 groups identified.