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Cb256o Web Only articles PtdIns(3)P 5-kinase activity and the maintenance of vacuolar size and membrane homeostasis. J. human phospholipase D1 wild-type and deletion mutants: is there evidence for an interaction of Cell Biol. 143, 65–79 (1998). phosphatidylinositol 4,5-bisphosphate with the putative pleckstrin homology domain? Biochim. 6. Cooke, F. T. et al. The stress-activated phosphatidylinositol 3-phosphate 5-kinase Fab1p is essential Biophys. Acta 1481, 189–201 (2000). for vacuole function in S. cerevisiae. Curr. Biol. 8, 1219–1222 (1998). 25. Hodgkin, M. N. et al. Phospholipase D regulation and localisation is dependent upon a phos- 7. Stack, J. H., DeWald, D. B., Takegawa, K. & Emr, S. D. Vesicle-mediated protein transport: regulato- phatidylinositol 4,5-bisphosphate-specific PH domain. Curr. Biol. 10, 43–46 (2000). ry interactions between the Vps15 protein kinase and the Vps34 PtdIns 3-kinase essential for pro- 26. Kim, Y. et al. 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The class II phosphoinositide ACKNOWLEDGEMENTS α 3-kinase PI3K-C2 is concentrated in the trans-Golgi network and present in clathrin-coated vesi- We thank D. G. S. Capelluto, J. L. Enmon, D. N. M. Jones and A. E. Wurmser for discussions and L. E. cles. J. Biol. Chem. 275, 11943–11950 (2000). Kay for pulse sequences. We also thank C. Sette, P. Iaquinta, J. Thorner, X. Song, W. Xu, A. Zhang, G. 23. Yokozeki, T., Kuribara, H., Katada, T., Touhara, K. & Kanaho, Y. Partially purified RhoA-stimulated Huang, X. Liang, J. V. Virbasius, M. P. Czech and G. W. Zhou for sharing unpublished data. We are sup- phospholipase D activity specifically binds to phosphatidylinositol 4,5-bisphosphate. J. Neurochem. ported by the Pew Scholars Program, University of Colorado Cancer Center and University of 66, 1234–1239 (1996). Colorado Health Sciences Center NMR Facility. 24. Hoer, A., Cetindag, C. & Oberdisse, E. Influence of phosphatidylinositol 4,5-bisphosphate on Correspondence and requests for materials should be addressed to M.O. Errata In the figure 4 legend, the sentence: “The structure of DPC is above the panel.” should be replaced by “The structure of DPC is inset into the panel, and the secondary structure is shown above the panel.” Also, in the figure 5 legend, “The conservation of structurally and functional- ly important PX domain residues are shown ...” should be replaced by “The conservation of structurally and functionally important PX domain residues is shown ...” This PDF posted on 4 June 2001 replaces the one originally posted on 24 May 2001. The cor- rected version of this paper will appear in the July issue of the journal. 618 NATURE CELL BIOLOGY VOL 3 JULY 2001 http://cellbio.nature.com © 2001 Macmillan Magazines Ltd.
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