Effect of Preharvest and Postharvest Application of Calcium on Banana Green-Life
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Technical paper Effect of preharvest and postharvest application of calcium on banana green-life 1 2 1 3 Philippe TIXIER *, Christophe BUGAUD , Rémy DUGUET , Frédéric SALMON 1 CIRAD, UPR 26, PRAM - Effect of preharvest and postharvest application of calcium on banana BP 214 - 97285 Lamentin green-life. Cedex 2 - Martinique, French West Indies, France, Abstract — Introduction. Fruit green-life is a major postharvest attribute on which conser- [email protected] vation on commodity chains depends. For climacteric fruits such as banana, green-life must be optimized to fit the transport and market requirements. Calcium was assumed to 2 CIRAD, UMR QUALISUD, strengthen cellular tissue and increase the green-life of fruits. Materials and methods.Inour PRAM - BP 214 - study, we tested the effect of preharvest and postharvest calcium applications on attributes of 97285 Lamentin Cedex 2 - banana fruit for two cultivars (Musa spp., AAA group, cv. Cavendish Grande Naine and Martinique, French West FLHORBAN920 cultivar, AAA group). Results and discussion. Extra calcium in fertilization Indies, France, of FLHORBAN920 cv. did not significantly affect the green-life of fruits (36 days) or the [email protected] hardness and thickness of the FLHORBAN920 cv. skin (63.7 N and 3.94 mm, respectively). The field calcium applications, by fertilization or spraying, did not alter pulp or peel chemical 3 CIRAD, UPR 75, Neufchâteau, 97130 composition. For postharvest treatments, the green-life of Cavendish and FLHORBAN920 Capesterre Belle-Eau - banana were not significantly altered by the presence or absence of calcium, or by the type of Guadeloupe, application; 45 and 26 days for the Cavendish and FLHORBAN920 cultivars, respectively. French West Indies, France, [email protected] Martinica / Musa (bananas) / keeping quality / preharvest treatment / postharvest control / calcium / soaking / spraying Effet de l’application de calcium avant et après la récolte sur la durée de vie verte de la banane. Résumé — Introduction. La durée de vie verte des fruits est une caractéristique post-récolte majeure des fruits ; elle définit leur capacité à se conserver au long de la chaîne de distribu- tion. Pour des fruits climactériques comme l’est la banane, la durée de vie verte doit être opti- misée afin de satisfaire les exigences de transport et de commercialisation. Le calcium est connu pour renforcer les tissus des plantes ainsi que leur durée de vie verte. Matériel et méthodes. Dans notre étude, nous avons testé l’effet d’application de calcium avant et après la récolte sur les caractéristiques post-récoltes de bananes de deux cultivars (Grande Naine and FLHORBAN920, Musa spp., AAA group, Cavendish). Résultats et discussion. L’ajout de calcium dans la fertilisation de la variété FLHORBAN920 n’a pas au d’effets significatifs sur la durée de vie verte (moyenne de 36 jours), ni sur la dureté et l’épaisseur de la peau des fruits (respectivement 63.7 N et 3.94 mm). L’application de calcium au champ par pulvérisation * Correspondence and reprints directe sur les fruits n’a pas modifié la composition chimique de la pulpe ni de la peau. Pour les applications de calcium après récoltes (par trempage), la durée de vie verte des fruits de Cavendish et de FLHORBAN920 n’ont pas été modifiés de manière significative par la pré- sence de calcium ni par le type d’application (durée de vie verte de 45 et 26 jours pour les fruits des variétés Cavendish et FLHORBAN920, respectivement). Martinique / Musa (bananes) / aptitude à la conservation / traitement avant Fruits, 2010, vol. 65, p. 1–9 récolte / lutte après récolte / calcium / trempage / pulvérisation © 2010 Cirad/EDP Sciences All rights reserved DOI: 10.1051/fruits/2010016 www.fruits-journal.org RESUMEN ESPAÑOL,p.9 Fruits, vol. 65 (4) 1 P. Tixier et al. 1. Introduction dish Grande Naine) and on a new triploid hybrid (FLHORBAN920 cultivar, AAA The green-life of climacteric fruits is the group). This hybrid is partially resistant to postharvest attribute on which their conser- Yellow Sigatoka and Black Leaf Streak Dis- vation on commodity chains depends, espe- ease [11]; it presents good tolerance to plant cially when there is a need for transport parasitic nematodes [12, 13], and has sen- between the producing and the selling area. sory and nutritional characteristics that are The green-life is the time between harvest different from those of Cavendish banana and the beginning of the climacteric rise. [14]. Such triploids are issued from conven- Banana fruits should be transported green tional breeding techniques [15], including and maturation should occur only at selling diploid resistant genitors previously dou- or in ripening stores. The stage of matura- bled with colchicine [16]. tion at which any fruit is harvested greatly The two varieties of banana were grown influences the green-life [1, 2]. Harvesting at in Martinique (French West Indies), on a plot an advanced stage of maturity is unsuitable located at 14° 48’ 16.0’’ N; 61° 01’ 58.5’’ W for fruits intended for long-distance ship- and 125 m altitude, on nitisol. The climate ment due to their shorter storage-life. The in this area is characterized by 2110 mm of earlier the fruits are harvested, the longer the rain annually and a mean temperature of storage-life, but the smaller the weight. 25.8 °C. Bunches were harvested at a pre- Farmers must thus optimize the green-life to cise physiological stage measured in heat fit the commodity chain requirements and units accumulated since the emergence of to maximize the yield. the flower, calculated in degree-days (dd). Postharvest techniques to increase green- FLHORBAN920 and Cavendish were har- life are mainly based on controlled atmos- vested at 1200 dd and 1000 dd, respectively. pheres or ethylene absorbers. Calcium coat- The heat units were calculated according to ing is also known to extend the shelf-life of a temperature base equal to 9 °C for fruits, e.g. apples [3]. Nevertheless, calcium FLHORBAN920 [17] and 14 °C for Caven- sprays on peach fruits had no effect on their dish [18]. shelf-life [4]. In postharvest application, cal- cium is assumed to be an extracellular stim- ulus that elicits calcium sensors involved in 2.2. Field trial plant defense [5]. Furthermore, calcium was shown to modify pectins and hemicellu- loses, which are known to influence the For the hybrid FLHORBAN920, we con- physical properties of fruits [6–9]. For ducted a field trial to test the effect, on the banana, no effect of calcium on fruit shelf- fruit green-life and fruit peel hardness and life has been shown [10]. thickness, of adding calcium in the fertiliza- tion and of spraying calcium in the field To our knowledge, no study has investi- directly on the bunch. Treatments included: gated the effect of calcium treatments on the (1) control with standard fertilization (100 g green-life of climacteric fruit in general and of complete fertilizer per plant per month; banana in particular. Herein, we present the 14% nitrogen; 7% phosphorous; 20% of pot- results of preharvest and postharvest appli- ash), (2) standard fertilization + calcium cations of calcium on the green-life of two added to soil (two times 50 g of CaCO3; cultivars of banana. We also measured the 2 months and 3 months before harvest; (3) effect of preharvest calcium applications on standard fertilization + one liter of a 5 g·L–1 peel and pulp postharvest characteristics. CaCO3 water solution sprayed directly on the bunch (two times, 2 months and 3 2. Materials and methods months before harvest). The calcium formu- lation was Megagreen® from Tribo Technol- ® 2.1. Banana varieties ogies , which is an activated form of calcium, including 14% CaCO2,8%SiO2 and Our study focused on the classic ‘Cavendish’ 3% MgO. Nine bunches were analyzed per cultivar (Musa spp., AAA group, cv. Caven- treatment. No difference between treatments 2 Fruits, vol. 65 (4) Effect of calcium on banana green-life was observed in the growth of banana essary to perforate the peel. The peel thick- plants, i.e., number and weight of fruits and ness was measured after harvest on green banana pseudo-trunk diameter. The period fruits, using a caliper rule. Finally, the peel of measurement was preformed over three and pulp chemical composition was meas- weeks in order to limit seasonal variation in ured for the different preharvest treatments. mineral composition [19]. We analyzed the K, Ca, Mg and P content of peel and pulp according to Martin- Prével’s methods [22]. 2.3. Pre-packing bath trial Clusters of four bananas issued from the 2.5. Statistical methods 2nd, 3rd and 4th banana hands from the top of the bunch were rinsed with alum sulfate To compare preharvest treatments, we per- (2 g·L–1) and dipped in fungicide (bitertanol, formed analyses of variance (ANOVA). Val- 200 mg·L–1) to prevent any postharvest dis- ues of green-life were subjected to a two- ease that could modify the green-life [20]. way analysis of variance that included the We tested the effect of postharvest cal- effects of the presence of calcium, of the cium application on the fruit green-life of treatments A to E, and interactions between FLHORBAN920 and Cavendish bananas. the presence of calcium and treatment. All Five treatments were used: (A) spray with statistical analyses were performed using the tested solution then no rinsing, (B) soak the R software [23]. for 1 min then rinsing with water, (C) soak for 10 min then rinsing with water, (D) soak for 100 min then rinsing with water, and (E) 3. Results and discussion soak for 100 min with no rinsing. All treat- ments were done with 0% and 5% of a 3.1.