Ultra Low Temperature Freezers

Installation and Operation

315673H01 August 2011

Visit us online to register your warranty www.thermoscientific.com/labwarranty IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result in damage to the unit, injury to operating personnel, and poor equipment performance. CAUTION All internal adjustments and maintenance must be performed by qualified service personnel.

Material in this manual is for informational purposes only. The contents and the product it describes are subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising from or related to the use of this manual.

Table of Contents

Safety Precautions...... 1 Unpacking ...... 2 Packing List...... 3 General Recommendations ...... 4 Temperature Monitoring ...... 4 General Usage...... 4 Initial Loading ...... 4 Operating Standards...... 5 Electrical Specifications...... 5 Installation ...... 6 Location...... 6 Wiring ...... 6 Leveling ...... 7 Backup System (Optional) ...... 7 Superinsulated Cabinet Construction...... 7 Door Operation ...... 7 Pressure Equalization Port...... 9 Installing the Remote Alarm Connector...... 9 Start Up...... 10 Initial Start Up...... 10 Unit Name (optional) ...... 11 Performance Mode...... 12 Security Mode...... 13 New User Entry ...... 14 Operation ...... 15 Operation Overview...... 15 Home Screen ...... 15 Using the Main Toolbar...... 17 Setting Temperature Set Points...... 17 Setting Preferences ...... 19 Managing Users ...... 20 Screen Calibration...... 23 Regional Settings...... 23 System Management ...... 24 User Log On/Off ...... 25 Health Status...... 26 Health Status Overview...... 26 Alarms...... 27 Temperature Sensor Screen...... 27 Event Log Screen ...... 28 Power Systems Screen ...... 29 Exporting Event and Temperature Data...... 30 Safety Precautions

1 Safety Precautions In this manual and on labels attached to this product, the words WARNING and CAUTION mean the following:

WARNING A potentially hazardous situation which, if not avoided, could result in serious injury or death.

CAUTION A potentially hazardous situation which, if not avoided, may result in minor or moderate injury or damage to the equipment.

Before installing, using or maintaining this product, please be sure to read this manual and product warning labels carefully. Failure to follow these instructions may cause this product to malfunction, which could result in injury or damage.

Below are important safety precautions that apply to this product:

• Use this product only in the way described in the product literature and in this manual. Before using it, verify that this product is suitable for its intended use. • Do not modify system components, especially the controller. Use OEM exact replacement equipment or parts. Before use, confirm that the product has not been altered in any way. • Your unit must be properly grounded in conformity with national and local electrical codes. Never connect the unit to overloaded power sources. • Disconnect the unit from all power sources before cleaning, troubleshooting, or performing other maintenance on the product or its controls.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 1 Unpacking

The following symbols are used in caution, warning and informational labels attached to the freezer:

caution, info on

electrical hazard off

hot surface watch hands

cold surface standby

protective conductor terminal

alternating earth ground current

2 Unpacking At delivery, examine the exterior for physical damage while the carrier’s representative is present. If exterior damage is present, carefully unpack and inspect the unit and all accessories for damage.

If there is no exterior damage, unpack and inspect the equipment within five days of delivery. If you find any damage, keep the packing materials and immediately report the damage to the carrier. Do not return goods to the manufacturer without written authorization. When submitting a claim for shipping damage, request that the carrier inspect the shipping container and equipment.

2 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Packing List

3 Packing List Inside the freezer cabinet is a bag containing:

• This manual • A CD with user’s manuals, including translated versions • Certificates of conformance and calibration • A remote alarm contact connector • Posts for rear spacing • A freezer key. If you have ordered a field-installed chart recorder, the bag will also contain: • Recorder installation instructions • Extra inkless paper If you have ordered a backup system, the cabinet will also contain: • A tube assembly • English and metric connectors If specified on the order, the bag may also include: • A QC temperature graph and test log • Calibration information If you have ordered the Proximity Access Card Option, the cards will be in a bag attached to the front of the freezer.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 3 General Recommendations 4 General Recommendations

4.1 Temperature Monitoring IMPORTANT NOTE: We recommend the use of a redundant and independent temperature monitoring system so that the freezer can be monitored continuously for performance commensurate with the value of product stored.

4.2 General Usage This refrigeration system is designed to maintain ultra-low temperatures with safety in an ambient environment up to+32°C (90°F), only when the freezer is used for storage.

WARNING This unit is not a “rapid-freeze” device. Freezing large quantities of liquid, or high-water content items, will temporarily increase the chamber temperature and will cause the compressors to operate for a prolonged time period.

Avoid opening the door for extended time periods since chamber temperature air will escape rapidly. Also, keep the inner doors closed as much as possible. When room air, which is higher in humidity, replaces chamber air, frost may develop in the chamber more rapidly.

4.3 Initial Loading Allow the freezer to operate at the desired temperature for a minimum of 12 hours before loading.

Load the freezer one shelf at a time, beginning with the top shelf. After loading each shelf, allow the freezer to recover to the desired set point before loading the next shelf. Repeat this process until the freezer is fully loaded.

CAUTION Failure to follow these procedures or overloading the unit may cause undue stress on the compressors or jeopardize user product safety.

4 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Operating Standards

5 Operating The freezers described in this manual are classified for use as stationary Standards equipment in a Pollution Degree 2 and Overvoltage Category II environment.

These units are designed to operate under the following environmental conditions:

• Indoor use • Altitude up to 2000m • Maximum relative humidity 60% for temperatures up to 32°C (90°F) • Main supply voltage fluctuations not to exceed 10% of the nominal voltage.

5.1 Electrical The last character in the model number listed on the dataplate identifies the Specifications electrical specifications for your unit. The voltage types are A, D, and V, as specified in the following table:

Table 1: Electrical Specifications

Model Voltage Frequency Current A 115V 60Hz 16A D 208/230V 60Hz 12A V 230V 50Hz 12A

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 5 Installation 6 Installation

CAUTION Improper operation of the equipment could result in dangerous conditions. To preclude hazard and minimize risk, follow all instructions and operate within the design limits noted.

6.1 Location Install the unit in a level area free from vibration with a minimum of eight inches (20 cm) of space on the top and sides, six inches (15 cm) in back. Refer to Section 6.3 for further instructions on leveling cabinets. Allow enough clearance so that door can swing open at least 85 degrees.

The rear spacing posts provided with the freezer can be used to ensure proper clearance. To install the spacing posts, screw them into the back in the rear deck area.

Do not position the equipment in direct sunlight or near heating diffusers, radiators, or other sources of heat. The ambient temperature range at the location must be 59 to 90°F (15 to 32°C).

6.2 Wiring

CAUTION Connect the equipment to the correct power source. Incorrect voltage can result in severe damage to the equipment.

CAUTION For personal safety and trouble-free operation, this unit must be properly grounded before it is used. Failure to ground the equipment may cause personal injury or damage to the equipment. Always conform to the National Electrical Code and local codes. Do not connect the unit to overloaded power lines.

CAUTION Do not position the unit in a way that impedes access to the disconnecting device or circuit breaker in the back of the unit.

Always connect the freezer to a dedicated (separate) circuit. Each freezer is equipped with a service cord and plug designed to connect it to a power outlet which delivers the correct voltage. Supply voltage must be within +10% or -10% of the freezer rated voltage.

CAUTION Never cut the grounding prong from the service cord plug. If the prong is removed, the warranty is invalidated.

6 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Installation

6.3 Leveling Make sure that the floor is level. The unit must be level both front to back and side to side.

300 and 400 box capacity models are equipped with two leveling legs on the right hand side. These must be lowered to ensure stability of the unit.

Be sure to set the brakes for units equipped with casters.

6.4 Backup System If you are using a CO2 or LN2 backup system, refer to Section 10 on (Optional) page 32 for installation and operation instructions.

6.5 Superinsulated In all models, the cabinet walls have a vacuum insulation core encapsulated Cabinet Construction by a sealed film laminate.

CAUTION Never drill holes in or near the cabinet walls. Drilling could damage the insulation and make the unit inoperable.

6.6 Door Operation Upright freezer models are equipped with an advanced assembly specifically designed for ultra-low temperature freezers.

Features include:

• One-hand operation • A front-accessible lock • Hasps for a standard padlock to provide additional security • Durable construction for reliable operation and safe product storage • Optional controlled access to the freezer with Proximity Access cards.

CAUTION When moving the freezer, always grasp cabinet surfaces; never pull the freezer by the latch handle.

Opening the Door For freezers with the Proximity Access Card option:

1. To unlock the door, pass the card in front of the freezer below the LCD display. 2. Grasp the latch handle and pull it toward yourself until the latch disengages from the cabinet strike. 3. Keep pulling by the latch handle to open the main door.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 7 Installation

For freezers without the Access Card option:

1. Remove the padlock if installed. 2. Grasp the latch handle and pull it toward yourself until the latch disengages from the cabinet strike. 3. Keep pulling by the latch handle to open the main door.

Opening the Door During a In case of power outage, you may use a 9 volt battery to activate the Power Outage proximity card access system. To access the 9 volt terminal, a piece of plastic trim must be removed near the user interface.

If the trim is gray, remove the left plastic trim by sliding it up and gently pulling it out.

If the trim is blue, slide the U-shaped trim piece down and gently pull it out.

Once the terminal is exposed, you can open the door by holding the 9 volt battery against the terminal and passing a valid proximity card below the display area.

Once the door is open, remove the battery.

Closing the Door Note that the latch does not self-engage automatically when you close the door. You must rotate the latch into the open position first.

1. Grasp the latch handle (preferably with your left hand) and pull it toward yourself, rotating the latch into the open position. 2. Move the freezer door into the closed position and gently push the handle away from you, making sure that the latch engages fully with the cabinet strike. 3. Keep applying gentle pressure to the latch handle until the latch is securely in closed position. 4. Insert the key and rotate counterclockwise to lock. 5. Replace the padlock as required.

8 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Installation

6.7 Pressure When an upright ultra-low temperature freezer door is opened, room Equalization Port temperature air rushes into the storage compartment. When the door is closed, the fixed volume of air is cooled rapidly. Pressure drops below atmospheric pressure, resulting in a substantial vacuum. Re-entry into the cabinet is impossible until internal pressures are returned to atmospheric pressure. Without a pressure equalization mechanism, it can take, in extreme cases, several hours before the door can easily be reopened.

All upright models feature a port that provides vacuum relief after door openings.

The pressure equalization port is located in the door behind the eye-level panel on the front of the freezer. Although the port is designed to self-defrost, excessive frost accumulation on the inner door could eventually restrict air flow. Therefore you should periodically inspect the inner door and brush away any loose frost using a stiff nylon brush.

6.8 Installing the The remote alarm contacts are located on the back of the freezer above and Remote Alarm to the left of the power switch. After installing the wiring from the remote Connector alarm to the connector, install the connector to the freezer microboard. When the unit is in the alarm condition:

• The leftmost pin (#7) is NO (normally open) output. • The second pin from the left (#6) is COM (common). • The third pin from the left (#5) is NC (normally closed). The contacts will trip in the event of a power outage, high temperature alarm or low temperature alarm.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 9 Start Up 7 Start Up

7.1 Initial Start Up To start up the freezer, complete the following steps:

1. Plug the freezer into the power outlet. 2. Turn the power switch in back of the freezer, on the bottom right, to the ON position. 3. When the freezer is powered up, you will see a logo displayed on the front screen for about 25 seconds, followed by a Regional Settings screen:

Figure 1. Regional Settings Screen

This screen allows you to specify the preferred display language along with date, time and temperature display preferences. If you prefer a display language other than English, press the correct radio button. Press the °F radio button if you want to change the display temperature from Celsius to Fahrenheit.

To set date, time, and 24hr/AM-PM display format, press each display you wish to change. Up and down arrows will then appear which you can use to change values.

On this and all other screens, a check mark icon will appear whenever you change values. Always be sure to press the check mark icon to confirm changes before navigating away from a screen.

Press the check mark icon to confirm changes. Press Next when ready.

10 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Start Up

4. A sequence of screens then asks you to verify that: • The freezer is properly grounded and connected to a dedicated power source

• The unit has sufficient clearance

• The unit is level.

• Ambient conditions are within the acceptable range.

If you are in doubt about whether any of the above installation requirements have been met, refer to the details in Section 6 and verify them before completing the startup procedure.

You will then be asked to specify unit name, performance mode, and security mode, as explained in the following sections.

7.2 Unit Name (optional) If desired, you can identify an individual freezer by specifying Unit Name on the screen below.

Figure 2. Unit Name Screen

The Contact Us information is pre-filled with Thermo Scientific information.

To enter unit name or contact information, press the field, then use the keyboard.

To edit unit name or contact information, press the pencil icon, then use the keyboard to edit.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 11 Start Up

The keyboard for this start up screen is English only.

• The Sym key switches to a symbol and numeric character set. To switch back to the alpha character set, press the up arrow (upper case). • The Clear key clears edits. • The Back key backs up one character. Press the check mark to confirm entry, then press Next to continue.

7.3 Performance Mode Specify Performance Mode on the screen below:

Figure 3. Performance Mode Screen

Use the radio button to change settings.

The default setting is High Performance Mode, which provides optimum temperature uniformity.

Energy Savings Mode is for customers who do not require maximum temperature uniformity. It can save up to 15% in energy usage.

If you change settings, press the check mark icon to confirm.

Press Next to continue.

12 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Start Up

7.4 Security Mode The next startup screen determines whether the freezer will run in the set point security mode. Choices are Secured and Full Access.

Figure 4. Security Mode Screen

The default setting is Full Access Mode, in which all users have access to all functions, including changing freezer set points.

Note that if you choose to run in Full Access mode and if you have the Proximity Access Card option installed, you will need to activate the cards after start up. Refer to Section 8.6 on page 20 for details.

You can choose Secured mode to enable set point security. In Secured mode, only an administrator can change freezer settings.

NOTE This Selection can only be made during the start up procedure. After start up, you can only change security mode by restoring factory defaults and shutting down the freezer. Refer to Section 8.5 on page 19.

If you wish to change the default, use the radio button to change to Secured. Press the check mark icon to confirm.

Press Next when ready.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 13 Start Up

7.5 New User Entry If you have selected Secured mode, you will need to create at least one account with Administrator privileges and the following screen will be displayed:

Figure 5. New User Entry Screen

• Name (required): 4-10 characters, case sensitive. Any valid keyboard characters are permitted. • Given Name (optional): 1-25 characters. • Password (required): 4-10 characters, case sensitive. Any valid keyboard characters are permitted. • Type (required): Select Admin. The first user must be an administrator. • E-mail (optional): Maximum 30 characters. • Telephone (optional): Maximum 20 characters, numeric only. • Access Card ID (optional). Proximity Access Card ID number. The Access Card field is active only if the Proximity Access Card option has been installed. To associate an access card with a user name, press the Access Card ID field and hold the card below the display. The card number will appear in the field and the card will be able to unlock the freezer door on subsequent card reads.

Press the corresponding X icon to delete a password or card ID.

Press the check mark icon to confirm entry, then Press Next to continue.

The start up wizard is now complete and the default Home Screen will be displayed. The start up screens shown in this section will not be displayed again unless the freezer has been turned off for 12 hours or the user has requested restoration of factory defaults.

14 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Operation 8 Operation

8.1 Operation Overview Once you have successfully completed the initial start up procedures, the freezer starts operating normally and the only actions required are:

• Setting the operating and alarm set points (Section 8.4 on page 17)

• Activating the CO2 or LN2 backup system if installed. For instructions on backup settings and activating the system, refer to Section 10 on page 32.

8.2 Home Screen The Home Screen below is the default screen.

Figure 6. Home Screen

The color of the heart at the top indicates health of the freezer: red indicates a serious alarm condition, yellow indicates a noteworthy condition, and blue is normal. Refer to the appendices at the end of this manual for full details on the conditions that determine the color of the heart icon and alarm activation.

A blinking heart indicates an active alarm or error condition.

IMPORTANT NOTE If the heart is red or yellow indicating an alarm or error condition, press the heart icon to bring up the health status screen (Section 9 on page 26) where you can view details on the alarm condition and mute audible alarms. Refer to Appendix A for details on alarms.

This screen displays the current cabinet temperature, the current temperature set point in green, and a graph showing recent readings.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 15 Operation

According to the default setting, the screen will also automatically go blank to save power between the hours of 9PM and 6AM. For instructions on changing the default to continuous display, refer to Section 8.5 on page 19. When a screen has gone blank, touch the screen anywhere to refresh the display.

Use the right and left arrows to scroll to different time periods. The graph displays up to two weeks of temperature data.

The graphic display can be expanded by pressing the plus sign icon on the upper right side of the Home Screen (refer to Figure 6 on page 15):

Figure 7. Expanded Graphic Display

Press an axis (temperature or Minutes) to adjust the scales.

Press the CSV icon at the lower left to download data to a USB flash drive. You can download both temperature and event log data. To create a CSV file, insert a USB flash drive in the USB port to the right of the display, then press the CSV icon. You will then be asked to specify date ranges.

Press the thermometer icon to select thermocouples for graphing (for details on temperature sensors, refer to Figure 20 on page 27).

16 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Operation

8.3 Using the Main The icons on the bluebar in the left side of the Home Screen provide access Toolbar to all functions as shown below:

Log On/Off

Settings Expand graph

Home

Health Status

Help

Figure 8. Main Toolbar

8.4 Setting Temperature To set temperature set points: Set Points 1. Press the Settings (gear) icon on the Main Toolbar shown in the figure below. The following screen will be displayed:

Freezer settings Warm alarm test Backup

Screen calibration

Regional settings

System Users

Preferences

Figure 9. Settings Screen

2. Press the Freezer Settings icon (the freezer at the top of the circle) to display the following screen:

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 17 Operation

Figure 10. Freezer Settings

3. All parameters on this screen may be adjusted by pressing the value displayed. Left and right arrows will then appear when you press a field, as will a check mark icon to confirm changes. Use the arrows to increase or decrease the values. 4. On this and all other screens, a check mark icon will appear whenever you change values. Always be sure to press the check mark icon to confirm changes before navigating away from a screen. 5. Press the back arrow at the bottom right to return to the previous screen. When secondary screens such as this one are displayed, the display will automatically return to the Home Screen if there is no user input within five minutes.

• Primary Set Point: Operating cabinet temperature. The minimum value is -50°C; maximum is-86°C. Factory default is -80°C. • Primary Offset: Used for calibration. Range is -10°C to +7°C. Default is 0. Note to calibrators: Customers performing on-site temperature calibration may observe as much as a 2°C variation when an external probe is placed next to the freezer control probe. This variation is due to optimization of the control system to ensure temperature uniformity throughout the chamber.

• Life Guard: Alarm setting for high internal (second sump) temperature. Range is 70°C to 98°C. Default is 94°C. • Extreme Ambient: Alarm setting for high ambient temperature. Range is 32°C to 40°C. Default is 37°C.

18 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Operation

• Warm alarm: -40°C to within 5°C of set point. Note that the warm alarm will be disabled for 12 hours from a warm start condition. • Cold alarm: -99°C to within 5°C of set point. • Time Delay: Specifies delay upon startup after power failure. Range is 0 to 20 minutes in steps of 6 seconds; default is 0.

8.5 Setting Preferences Pressing the Preferences icon (Figure 9 on page 17) brings up the following screen:

Figure 11. Preferences

Use the radio buttons to make selections, and confirm choices by pressing the check mark icon.

• Screen Auto Off: 9PM to 6AM darkens screens after hours. If you change to None, screen are constantly illuminated. • Power Mode: The default setting is High Performance Mode, which provides optimum temperature uniformity. Energy Savings Mode is for customers who do not require maximum temperature uniformity. If you wish to change this setting, note that it will be necessary to power down and restart the freezer. • Screen Intensity: Controls the brightness of the screens. • Graph Duration: Changes the x axis of the home screen graph. Choices are 2, 4, or 6 hours.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 19 Operation

• Reset Factory Settings: Restores all freezer settings to factory default values. NOTE The Reset Factory Settings option has the effect of deleting ALL freezer settings and user databases. Be sure to save the configuration to preserve settings and users (see Section 8.9 on page 24).

• Audible Ring Back: Determines time to mute an active alarm. A muted alarm will sound again after the specified time elapses, 15, 60, or 180 minutes.

8.6 Managing Users User management is used for two functions:

User Management: If running in Secured Mode, use the screens to manage users.

Access Card Assignment: If the freezer has the Access Card option installed, use the User Management screens to program access,

Secured Mode Pressing the Users icon (see Figure 9 on page 17) brings up the following screen:

Figure 12. User management (Secured mode)

To edit or delete a user, press the line and then press the appropriate ion on the right:

• Pencil Icon: Edit a user. • X: Delete a user. To add a user, press the star icon.

20 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Operation

Managing Users When logged on as an administrator while running in Secured Mode, the following screen appears:

Figure 13. Edit User entry (Secured mode)

Press a field to edit or create a new user entry.

Press the corresponding X icon to delete or change a password or card ID.

• User Name (required): 4-10 characters, case sensitive. Any valid keyboard characters are permitted. • Given Name (optional): 1-25 characters. • Password (required): 4-10 characters, case sensitive. Any valid keyboard characters are permitted. • Type (required): Admin or User. • E-mail (optional): Maximum 30 characters. • Telephone (optional): Maximum 20 characters, numeric only. • Access Card ID (optional). Proximity Access Card ID number. This can be added or changed following the procedures described in the next section. If you are adding multiple users, press the left arrow to return to the User Management Screen, press Star, and repeat the process.

Be sure to confirm changes by pressing the check mark icon.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 21 Operation

Managing Access Cards Press the Users icon (see Figure 9 on page 17) to bring up the User Management screen. Then press the star icon to add a user, the pencil or X to edit or delete a user.

The following screen appears:

Figure 14. User Entry Screen (Full Access Mode)

Press the User Name field and enter the name using the keyboard. The name is 4-10 characters, case sensitive.

To associate an access card with a user name, press the Access Card ID field and hold the card below the display. The card number will appear in the field and the card will be able to unlock the freezer door on subsequent card reads.

If you are managing card access for multiple users, press the left arrow to return to the User Management Screen and repeat the process.

Note that access cards are not linked to set point security. They only unlock the door.

Be sure to confirm changes by pressing the check mark icon.

22 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Operation

8.7 Screen Calibration Pressing the Calibration icon (see Figure 9 on page 17) brings up the following screen:

Figure 15. Screen Calibration

Touch the calibration points at each corner, one at a time, and press OK when you have touched all four corners.

8.8 Regional Settings Pressing the System icon (see Figure 9 on page 17) brings up the following screen, which displays the same parameters as the screen displayed at the beginning of the initial startup procedure:

Figure 16. Regional Settings

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 23 Operation

If you prefer a display language other than English, press the correct radio button. Press the °F radio button if you want to change the display temperature from Celsius to Fahrenheit.

To set date, time, and 24hr/AM-PM display format, press each numeric display you wish to change. Up and down arrows will then appear which you can use to change values.

Click the check mark icon to confirm changes and press Next when ready.

8.9 System Management Pressing the System Management icon (see Figure 9 on page 17) brings up the following screen:

Figure 17. System Management Settings

Use this screen to upload and download configurations using a USB flash drive. The USB port is directly to the right of the display.

Configuration files may be uploaded to other freezers.

Maximum power rating for USB flash drives is 500mA.

The Copy Files option is used to copy files to the SD card (user interface).

For information on exporting temperature and event data, refer to Section 9.6 on page 30.

24 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Operation

8.10 User Log On/Off Use the key icon on the left bluebar to log on or log off.

Figure 18. User Log On

When the system is running in Secured mode, a password field will appear upon name entry.

Press the check mark icon to confirm entry, then Press Next to continue.

When the system is running in Full Access mode, this screen is used for service log on and log off functions.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 25 Health Status 9 Health Status

9.1 Health Status Pressing the Health Status (heart) icon on the main toolbar displays the Overview following screen:

Figure 19. Health Status Screen

This screen reports door openings, temperature excursions, ambient conditions, BUS (backup system) status, and recent error and alarm conditions.

• Door openings: Number of door openings since last reset. Last One displays date and time of the last door opening. Press the Reset icon (the circle with arrows) to zero the door openings display. • Temp excursions: Actual is current cabinet temperature. Warm and Cold are highest and lowest temperatures recorded since the last reset. Press the Reset icon (the circle with arrows) to clear the warm and cold values. • Conditions: Current ambient temperature and line voltage. • BUS status: Displayed only if backup system is installed. Red denotes low tank level; green is OK. Injecting/Not Injecting reports whether the backup is active. Recent error and alarm conditions are listed on the right side.

You can press the temperature, lightning bolt, and pen-and-book icons to bring up secondary screens with more detailed health status information. These secondary screens are discussed in Sections 9.3 through 9.5 below.

Press the left arrow to exit and return to the Home Screen.

26 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Health Status

9.2 Alarms The Health Status Screen is the screen to use to manage alarm conditions. When an audible alarm is sounding, you can view the event log to see what is causing the alarm, and press the bell icon on the lower right to silence it.

The text field next to the bell icon indicates three possible conditions:

• System OK: No active or has-been alarm conditions • Active: Alarm condition, press the bell icon to mute audible alarms • Past Event: The bell icon is replaced by an X. Press the X to acknowledge that the past events have been recorded in the event log. A red heart on the home screen indicates a serious alarm condition which must be corrected, such as a warm alarm or a power failure. A yellow heart indicates less serious warning conditions, such as open doors and “has-been” alarms.

For full details on alarm conditions, refer to Appendix A on page 39.

9.3 Temperature Sensor Pressing the Sensor icon displays: Screen

Figure 20. Temperature Sensor Screen

This screen displays the temperature readings at the sensors in the cabinet and on the compressor deck. The cabinet sensors are (top to bottom): TC 3: Evaporator inlet RTD: Control sensor (main cabinet temperature) TC 4: Evaporator outlet

The compressor deck sensors are (clockwise from the top):

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 27 Health Status

TC 10: Heat exchanger TC 1: Fist stage suction TC 6: Second stage suction TC 9: Second stage sump TC 7: Liquid line TC 2: Condenser air inlet

9.4 Event Log Screen Pressing the book-and-pen icon displays:

Figure 21. Event Log Screen

This screen displays up to two weeks of recent events, with date and time stamps for each event.

The Date and Type columns can be sorted ascending or descending by pressing on the column header.

You can also view details for an individual event by pressing on a single row.

The icons on the right side are filters:

• ALL displays all events. • The bell icon displays only alarm conditions. • The door icon displays only door opening events. • The users icon displays only user events. • The battery icon displays only battery events. • The bottom icon displays only BUS (backup system) events.

28 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Health Status

Press the CSV icon at the lower left to download data to a USB flash drive. To create a CSV file, insert a USB flash drive in the USB port to the right of the display, then press the CSV icon. You will then be asked to specify file names and date ranges.

9.5 Power Systems Pressing the lightning bolt icon displays: Screen

Figure 22. Power Systems Screen

The Power Modes area displays mode of operation (buck, boost or normal), input voltage, and output voltage.

In the Main and Backup battery areas, the “months” display shows the number of months remaining until the next recommended battery change. The bars to the left indicate voltage.

When changing a battery, press the Change Icon (the circle with arrows) in the lower right corner of the appropriate box. After a confirm dialog, the months to replace value is reset to 12.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 29 Health Status

9.6 Exporting Event and The freezer records up to 15 years of temperature and event data. Data can Temperature Data easily be downloaded to a flash drive via the USB port.

Downloading Event Data To download event log information:

1. Insert a USB flash drive in the USB port located to the right of the display. 2. Press the CSV icon at the lower left of the Event Log screen (Figure 21 on page 28). The following screen appears:

Figure 23. Export Event Log

3. Press the Year and Month fields to specify date ranges. 4. Press the icon to the right of the From fields when ready. You will get an error message if no USB flash drive is present. 5. If you wish to export another event log, press the CSV icon at the bottom of the screen and repeat the process. 6. Press the left arrow to exit.

30 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Health Status

Downloading To download temperature data: Temperature Data 1. Insert a USB flash drive in the USB port located to the right of the display. 2. Press the CSV icon at the lower left of the Expanded Graph screen (Figure 7 on page 16). The following pop-up appears:

Figure 24. Export Temperature Log

3. Press the year and month fields to specify date ranges. 4. Press the icon to the right of the From fields when ready. You will get an error message if no USB flash drive is present. 5. Press the left arrow to exit.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 31 Backup System (Optional)

10 Backup System When you purchase a built-in CO2 or LN2 optional backup system for the (Optional) freezer, backup control is integrated into the main user interface.

Note: : Always purchase the cylinders which are equipped with siphon tubes for withdrawing liquid from the bottom of the cylinder. CO2 cylinders must be kept at room temperature to function properly. LN2 bottles are functional at any reasonable temperature.

10.1 CO2 and LN2 The following are precautions for using liquid CO2 and LN2 backup Precautions systems.

WARNING If a CO2 or LN2 cylinder falls and a valve is knocked off, the cylinder becomes a deadly and completely unguided missile. Transport the cylinders in a handtruck or cart with secure chain ties for the cylinder. After cylinders are connected to the equipment, securely attach them with chains to a solid, stationary object such as a building column.

WARNING CO2 and LN2 liquids are non-poisonous but are very cold and will burn unprotected skin. Always wear protective eyewear and clothing when changing cylinders or working on the piping systems attached to an active source of liquid refrigerant.

WARNING The gases produced by evaporation of CO2 or LN2 are non-poisonous but displace the oxygen in a confined space and can cause asphyxiation. Do not store the cylinders in subsurface or enclosed areas.

CAUTION When closing the cylinder valve, make sure that the injection solenoid is energized to allow all the liquid to bleed off instead of being trapped in the supply tubing. Failure to do this results in activation of the pressure relief device, which could damage the freezer and requires replacing if it is activated.

CAUTION : For models ordered with factory installed built-in backup systems, the flow of liquid CO2 or LN2 will be discontinued if the door is opened during operation of the backup system. For units operated with free-standing, field installed type backup system, the flow of liquid CO2 or LN2 will be discontinued upon door opening only if the switch provided with the free-standing package is installed on the freezer.

32 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Backup System (Optional)

10.2 Installation Field installed systems are supplied with complete installation and operating instructions. If your system is factory installed, the freezer is shipped with a coiled length of tubing to connect the freezer to the bottles:

• 3/8 in. OD or the corresponding metric copper tubing for connection to the CO2 supply. • 5/8 in. OD or the corresponding metric copper tubing covered with TM Armaflex insulating tubing for connection to the LN2 supply. Straighten the coiled tubing and connect one end to the labeled connection on the freezer and the other end to the supply bottle or building supply fitting.

10.3 Start Up To activate the backup system;

1. Follow the instructions in Section 7 to power on the freezer and set temperature and alarm set points. 2. Enter the backup set point and backup type on the Backup Setting Screen below, which you can bring up by pressing the tank icon on the Freezer Settings Screen (shown in Figure 10 on page 18):

Figure 25. Backup Settings Screen

3. Press the check mark icon to confirm changes.

4. Turn on the CO2 or LN2 supply.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 33 Backup System (Optional)

10.4 Operation When the backup system is in operation, you can view and reset parameters on the settings screen).

Once the backup system has been activated, you can test it by pressing the Test Injection button. The system will inject as long as you keep pressing.

The backup system can run for a minimum of 24 hours on battery power.

34 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Chart Recorders (Optional)

11 Chart Recorders Panel-mounted six-inch seven-day recorders are available as options for all (Optional) freezer models except for the smallest (300 box capacity) models.

11.1 Set Up and To prepare the recorder to function properly, complete the following steps: Operation 1. Open the recorder door to access the recorder. 2. Install clean chart paper (refer to Section 11.2 below). 3. Remove the plastic cap from the pen stylus and close the recorder door.

Recorder operation begins when the system is powered on.The recorder may not respond until the system reaches temperatures within the recorder’s range.

Chart Buttons

Imprinting Stylus

Reference Mark

Chart Hub-Nut and Retaining Wire

Figure 26. Chart Recorder

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 35 Chart Recorders (Optional)

CHANGE CHART 3

Figure 27. Chart Buttons

CAUTION : Do not use sharp or pointed objects to depress the chart buttons. This may cause permanent damage to the recorder.

11.2 Changing Chart To change the chart paper, complete the following steps: Paper 1. Locate the pressure sensitive buttons at the front, upper left of the recorder panel. 2. Press and hold the Change Chart button (#3) for one second. The pen will move off the scale. 3. Unscrew the center nut, remove the old chart paper, and install new chart paper. Carefully align the day and time with the reference mark (a small groove on the left side of the recorder panel). 4. Replace the center nut and hand tighten. Press the Change Chart button again to resume temperature recording.

11.3 Calibration This recorder has been accurately calibrated at the factory and retains Adjustment calibration even during power interruptions. If required, however, adjustments can be made as follows:

1. Run the unit continuously at the control set point temperature. Continue steady operation for at least two hours to provide adequate time for recorder response. 2. Measure cabinet center temperature with a calibrated temperature monitor. 3. Compare the recorder temperature to the measured cabinet temperature. If necessary, adjust recorder by pressing the left (#1) and right (#2) chart buttons.

Note: The stylus does not begin to move until the button is held for five seconds.

36 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Maintenance and Troubleshooting

12 Maintenance and Unauthorized repair of your freezer will invalidate your warranty. Contact Troubleshooting Technical Service at 1-800-438-4851 for additional information.

CAUTION Maintenance should only be performed by trained personnel.

12.1 Cleaning the Clean the condenser at least every six months; more often if the laboratory Condenser area is dusty. There are two condenser filters: a main filter and a lower filter for extra air flow into the condenser.

To clean the condenser, complete the following steps:

1. Pull the grill open (300 and 400 box capacity models) or slide it to the right (larger models). 2. Remove both filters. 3. Vacuum the condenser. 4. Replace the filters and close the grill.

12.2 Cleaning the Clean the condenser filters every two or three months. Condenser Filter 1. Pull the grill open (300 and 400 box capacity models) or slide it to the right (larger models). 2. Remove the filter. 3. Shake the filter to remove loose dust, rinse the filter in clean water, shake the excess water from the filter, and replace the filter. 4. Close the grill.

12.3 Gasket Periodically check the gaskets around the door for punctures or tears. Leaks Maintenance are indicated by a streak of frost which forms at the point of gasket failure. Make sure that the cabinet is level (refer to Section 6.3 on page 7 for leveling information).

Keep the door gaskets clean and frost free by wiping gently with a soft cloth.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 37 Maintenance and Troubleshooting

12.4 Defrosting the Defrost the freezer once or year or whenever the ice buildup exceeds 3/8”. Freezer To defrost, complete the following steps:

1. Remove all products and place in another cabinet. 2. Turn off the freezer. 3. Open the outer door and all inner doors. 4. Let the freezer stand with doors open for at least 24 hours. This allows both the interior and foamed refrigerant system to warm to room temperature. 5. Dispose of the ice and wipe out any water standing in the bottom of the cabinet. 6. If there is freezer odor, wash the interior with a solution of baking soda and warm water. Clean the exterior with any common household cleaning wax. 7. Close the doors, restart the freezer and reload, following the instructions in Section 4.3 on page 4.

12.5 Alarm Battery Have a certified technician replace the alarm battery every twelve months at Maintenance most, and check the condition of the battery frequently by viewing the Power Systems screen (see Figure 16). Be sure to reset the timer when the battery is replaced.

38 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Warranty

13 Warranty Be sure to register your warranty online:

www.thermoscientific.com/labwarranty

THERMO FISHER SCIENTIFIC FREEZER WARRANTY

The Warranty Period starts two weeks from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the warranty period.

During the first two years of the warranty period, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo's expense, labor included. The ULT Freezers include an additional three year warranty on the compressors, parts only, F.O.B. factory. Installation and calibration is not covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to any work being performed. Expendable items, i.e., glass, filters, pilot lights, light bulbs and door gaskets are excluded from this warranty.

Replacement or repair of component parts or equipment under this warranty shall not extend the warranty to either the equipment or to the component part beyond the original two year warranty period. The Technical Services Department must give prior approval for the return of any components or equipment.

THIS WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES, WHETHER WRITTEN, ORAL, OR IMPLIED. NO WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE SHALL APPLY. Thermo shall not be liable for any indirect or consequential damages including, without limitation, damages relating to lost profits or loss of products.

Your local Thermo Scientific Sales Office is ready to help with comprehensive site preparation information before your equipment arrives. Printed instruction manuals carefully detail equipment installation, operation, and preventive maintenance.

If equipment service is required, please call your Technical Services Department at 1-800-438-4851 (USA and Canada). We're ready to answer your questions on equipment warranty, operation, maintenance, service, and special applications. Outside the USA, contact your local Thermo Scientific office or distributor for warranty information.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 39 Warranty

THERMO FISHER SCIENTIFIC FREEZER INTERNATIONAL DEALER WARRANTY

The Warranty Period starts two months from the date your equipment is shipped from our facility. This allows for shipping time so the warranty will go into effect at approximately the same time your equipment is delivered. The warranty protection extends to any subsequent owner during the warranty period. Dealers who stock our equipment are allowed an additional four months for delivery and installation, providing the warranty card is completed and returned to the Technical Services Department.

During the first two years of the warranty period, component parts proven to be non-conforming in materials or workmanship will be repaired or replaced at Thermo's expense, labor excluded. The ULT Freezers include an additional three year warranty on the compressors, parts only, F.O.B. factory. Installation and calibration is not covered by this warranty agreement. The Technical Services Department must be contacted for warranty determination and direction prior to any work being performed. Expendable items, i.e., glass, filters, pilot lights, light bulbs and door gaskets are excluded from this warranty.

If equipment service is required, please contact your local Thermo Scientific office or local distributor.

We're ready to answer your questions on equipment warranty, operation, maintenance, service, and special applications. Outside the USA, contact your local Thermo Scientific office or distributor for warranty information.

40 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Appendix A: Alarm Summary Appendix A: Alarm Summary

Alarm Summary Alarms/Warnings Audible Remote Visual Note (summary of alarm) Alarm (Tone) BPHX Persistent Temp Out Yellow Heart Active heat exchange temp out condition which has deactivated the 2nd stage Buck/Boost Insufficient Yellow Heart Input voltage out of range and cannot Correction be corrected for. BUS Battery Low Yellow Heart BUS battery low voltage condition. BUS Battery PM Expired A one time dialog reminds the user to change the battery. This message will appear for 24 hours or until acknowledged and does not impact control. Clean Filter Yellow Heart Persistent >10°C persistent temperature delta (condenser air inlet and condenser out temperature) Cold Alarm Tone 1 X Red Heart Primary RTD < set point (measured in 0.01C) Cold Alarm Has-been Yellow Heart Must be acknowledged for yellow heart to clear Door Ajar Tone 2 Red Heart Activates after 180 seconds of an open door condition (sensed by system door switch) Extreme Ambient Yellow Heart Condenser air inlet temperature > user programmed value (default 36.7°C) Inability to Attain Set point Yellow Heart Activates after NDO >12hrs without attaining cycle Lifeguard Yellow Heart 2nd stage sump temperature > user programmed value (default 94°C) Power Failure Tone 1 X Red Heart Unit in power failure mode (running on battery).

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 41 Appendix A: Alarm Summary

Power Failure Has-been Yellow Heart Must be acknowledged for yellow heart to clear Stuck Button Yellow Heart Stuck button is detected on a continuous press from any screen and times out to the Home Screen. System Battery Low Yellow Heart System battery low voltage condition. System Battery PM Expired A onetime dialog reminds the user to change the battery. This message will appear for 24 hours or until acknowledged and does not impact control. UI/Main Lost Tone 3 X Yellow Heart Primary RTD shows “----“ Communications (from Other Sensor outputs show blank UI) Other settable parameters retain their value prior to lost communications Warm Alarm Active Tone 1 X Red Heart Primary RTD > Set point (measured in 0.01C) Warm Alarm Has-been Yellow Heart Must be acknowledged for yellow heart to clear

42 Installation and Operation Manual Thermo Scientific Ultra Low Temperature Freezers Appendix B: Event Log Detail

Event Log Entry Detail Event Name Event Log Event Field – Intelligent Text Detail Type/Icon

Buck/Boost Insufficient Alarm Input line voltage, compensated voltage and current Correction Buck/Boost sate. BUS Battery Low Alarm + BUS Voltage of the backup system battery. + Battery Clean Filter Alarm Condenser air temperature and the condenser liquid line out temperature. Cold Alarm Active Alarm Primary RTD temperature that first caused the alarm as communicated via the Main when the alarm is first sensed, cabinet set point and the status of each compressor (on/off). Cold Alarm Has-been Alarm Primary RTD temperature that first caused the has-been condition, cabinet set point and the status of each compressor (on/off). Door Ajar Alarm + Primary RTD temperature, logged in user (if applicable) and Door HID user (if applicable) Extreme Ambient Alarm Condenser air temperature. Inability to Attain Set point Alarm Primary RTD temperature. Lifeguard Alarm 2nd stage sump temperature. Persistent Warm Heat Exchange Alarm Heat Exchange temperature. Power Failure Active Alarm Current system voltage and time delay setting. Power Failure Has-been Alarm Current system voltage and time delay setting. System Battery Low Alarm + Voltage of the system battery. Battery UI/Main Lost Communications Alarm Last valid RTD temperature. Warm Alarm Active Alarm Primary RTD temperature that first caused the alarm as communicated via the Main when the alarm is first sensed, cabinet set point and the status of each compressor (on/off). Warm Alarm Has-been Alarm Primary RTD temperature that first caused the has-been condition as communicated via the Main when the has-been is first sensed, cabinet set point and the status of each compressor (on/off). Warm Pull Down Verification Event Records that the system pulled down successfully recording the 10°C slopes from 0°C to set point. Add User Event User New username and role. Buck/Boost Stage Change General Non-compensated voltage at the time of performance mode change, the compensated voltage at the time of mode change and the state from/to. BUS Battery PM Expired Battery Battery replacement period expired. BUS Battery PM Reset User + BUS Value of the counter prior to reset and who performed the + Battery reset (secure mode) BUS Set Point Setting Change User + BUS User who made the change (secure mode only) and the to/from BUS set point. BUS Type Change User + BUS User who made the change (secure mode only) and the to/from BUS type. Change/Set RTC (Time) User To/from time. Cold Alarm Setting Change User User who made the change (secure mode only) and the to/from cold alarm set point. Door Closed Door Open duration. Door Count Reset User Door open count that was cleared. Door Open Door Who opened (when the HID option is used). If no HID is user and there is a user logged in, that username is displayed. Edit User Event User New user information. Event Log Rollover General Earliest date remaining in the Event Log. Firmware Upgrade (Main / UI) User To/from version information. Offset Setting Change User User who made the change (secure mode only) and the to/from offset. Power Down User User and date/time of the power down. Remove User Event User User and user removed. Sensor: Out of Range (OOR) General Which sensor(s) are currently out of range. Set Point Setting Change User User who made the change (secure mode only) and the to/from set point. Appendix B: Event Log Detail

Switch Mode Fully Accessible / User User who made the change (secure mode only) and the Secure Mode to/from operational mode. System Battery PM Expired Battery Battery replacement period expired. System Battery PM Reset User + Value of the counter prior to reset and who performed the Battery reset (secure mode) Temperature Excursion Reset User User who reset and the value reset to. Temperature Log Rollover General The earliest date remaining in the Temperature Log. User Login/Off User Username and indicate if the logoff was a timeout Voltage/Frequency Mismatch General Measured voltage and frequency. Warm Alarm Setting Change User User who made the change (secure mode only) and the to/from warm alarm set point.

Thermo Scientific Ultra Low Temperature Freezers Installation and Operation Manual 45 Important

For your future reference and when contacting the factory, please have the following information readily available:

Model Number:

Serial Number:

Date Purchased:

The above information can be found on the dataplate attached to the equipment. If available, please provide the date purchased, the source of purchase (the manufacturer or specific agent/rep organization), and purchase order number.

IF YOU NEED ASSISTANCE:

LABORATORY PARTS and SERVICE Phone: 800/438-4851 TECHNICAL SUPPORT Phone: 800/438-4851