Nov. 2013. Vol. 1, No.1 ISSN 2311-2476 International Journal of Research In Agriculture and Food Sciences © 2013 IJRAFS & K.A.J. All rights reserved http://www.ijsk.org/ijrafs.html PRELIMINARY RESULTS FOR USE SSR MARKERS IN BITTER VETCH “VICIA ERVILIA (L.) WILLD” Salama El Fatehi1,2, Gilles Béna2,4, Laïla Sbabou2,3, Abdelkarim Filali-Maltouf2,3 and Mohammed Ater1,2 1, Laboratoire de Diversité et Conservation des Systèmes Biologiques (LDICOSYB), Université Abdelmalek Essaâdi, P.B. 2121, Tétouan, Maroc.
[email protected]. 2, Laboratoire Mixte International (LMI), Université Mohamed V-Agdal. Avenue Ibn Batouta BP 1014, Rabat, Maroc. 3, Laboratoire de Microbiologie et Biologie Moléculaire, Université Mohamed V-Agdal, Avenue Ibn Batouta BP 1014, Rabat, Maroc. 4, Laboratoire des Symbioses Tropicales et Méditerranéennes, Institut de Recherche pour le Développement (IRD), Campus International de Baillarguet, 34398 Montpellier Cedex 5, France. ABSTRACT A development of genomic DNA extraction protocol from the leaves of Vicia ervilia was conducted. The extraction from fresh material gives better outcomes than extraction from dried leaves. The DNA extracts obtained have significantly higher quality (ratio A260/280 is 1.99 and A260/230 is 1.92). The yields quantity of extracted DNA is also superior (1030 ng/µl). Using enriched genomic bank, sequences containing microsatellite were isolated. Twenty-four pairs of primers allowing the amplification products that are easy to read have been obtained, nine of which have revealed the polymorphism. The average value of the index PIC (Polymorphism Information Content) is 0.65 and the average number of alleles per locus is 7.6. These values prove to be more interesting compared to the available data in leguminous plants.