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Implication of IGF1R in Murine Acute Lung Inflammation and House Dust Mite-Induced Allergy

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Implication of IGF1R in Murine Acute Lung Inflammation and House Dust Mite-Induced Allergy TESIS DOCTORAL Título Implication of IGF1R in murine acute lung inflammation and house dust mite-induced allergy Autor/es Sergio Piñeiro Hermida Director/es José Manuel García Pichel Facultad Facultad de Ciencia y Tecnología Titulación Departamento Curso Académico Implication of IGF1R in murine acute lung inflammation and house dust mite- induced allergy, tesis doctoral de Sergio Piñeiro Hermida, dirigida por José Manuel García Pichel (publicada por la Universidad de La Rioja), se difunde bajo una Licencia Creative Commons Reconocimiento-NoComercial-SinObraDerivada 3.0 Unported. Permisos que vayan más allá de lo cubierto por esta licencia pueden solicitarse a los titulares del copyright. © El autor © Universidad de La Rioja, Servicio de Publicaciones, 2018 publicaciones.unirioja.es E-mail: [email protected] Thesis for doctoral degree (Ph.D.) 2017 IMPLICATION OF IGF1R IN MURINE ACUTE LUNG INFLAMMATION AND HOUSE DUST MITE-INDUCED ALLERGY Sergio Piñeiro Hermida Center for Biomedical Research of La Rioja (CIBIR) Lung Cancer and Respiratory Diseases Unit, Logroño, Spain IMPLICATION OF IGF1R IN MURINE ACUTE LUNG INFLAMMATION AND HOUSE DUST MITE-INDUCED ALLERGY Sergio Piñeiro Hermida Logroño 2017 José Manuel García Pichel, Ph.D. in Biological Sciences and head of the Lung Cancer and Respiratory Diseases Unit from the Center of Biomedical Research of La Rioja, Certify that: The present thesis entitled “Implication of IGF1R in murine acute lung inflammation and house dust mite-induced allergy” undertaken by Sergio Piñeiro Hermida, B.Sc. in Biology and M.Sc. in Forensic Sciences, meets the conditions of originality required to qualify for the international doctorate mention from the Universities of La Rioja and Zaragoza. Logroño, 11 December 2017. José M. García Pichel, Ph.D. Head of the Lung Cancer and Respiratory Diseases Unit Center for Biomedical Research of La Rioja (CIBIR) 26006 Logroño, Spain Tel.: + (34) 941278855 Email: [email protected] Á miña familia e en especial ó meu pai, por ensinarme o que é a constancia, a humildade e a xenorosidade AGRADECIMIENTOS Quien lo iba a decir, cuatro años después aquí estoy escribiendo los agradecimientos de mi tesis, algo que ni me imaginaba que ocurriría. Después de terminar mi máster y tras pasar por varios laboratorios sin un proyecto definido ni financiación, estuve a punto de abandonar la investigación. Debido a esto, todas mis esperanzas por encontrar financiación para desarrollar mi carrera investigadora estaban por los suelos. Anteriormente, había estado matriculado en un programa de doctorado en la Universidad de Santiago de Compostela, cuyo proyecto se estaba desarrollando en el “Grupo de Medicina Genómica” del Hospital Clínico. Debido a que estaba en una lista de correos del hospital, todavía me seguían llegando emails de charlas, ofertas de trabajo, etc. y en Octubre de 2013 recibí un email en el que se anunciaba un contrato para la realización de la tesis en la “Unidad de Cáncer de Pulmón y Enfermedades Respiratorias del CIBIR”. Después del proceso de selección, en el que se tuvo en cuenta tanto la experiencia de cada candidato como la entrevista personal, me quedé a las puertas de conseguir el contrato. Pocos días después de la resolución del proceso de selección, recibí una llamada (concretamente el viernes 6 de Diciembre de 2013) en la que se me comunica que el contrato era para mí porque la persona a la que se le había concedido había renunciado. Ese fue uno de los días más felices de mi vida! En primer lugar me gustaría agradecer a la Consejería de Industria, Innovación y Empleo del Gobierno de la Rioja la aportación económica necesaria para mi incorporación a la Unidad de Cáncer de Pulmón y Enfermedades Respiratorias del CIBIR. También me gustaría dar las gracias a la Fundación Rioja Salud por las maravillosas instalaciones del CIBIR, donde se ha desarrollado la mayor parte de mi tesis. En particular me gustaría dar las gracias a las siguientes personas: A José Pichel, mi director de tesis, por darme la oportunidad de incorporarme a su grupo de investigación, por sus consejos y apoyo durante estos 4 años y por creer en mi “intuición investigadora” cada vez que proponía hacer algo nuevo. A Raquel por su maravilloso trabajo con las histologías, el cual se ve reflejado en las últimas publicaciones del grupo y que son un valor añadido a cualquier trabajo debido a su gran profesionalidad. A Icíar por hacerme sentir como en casa cuando llegué al laboratorio y por enseñarme la dinámica de trabajo del grupo, debido a que todo era nuevo para mí, ya que venía de otro ámbito investigador diferente. Muchas gracias por tu apoyo a nivel investigador durante estos 4 años, sobre todo en los momentos más críticos como son las revisiones de los “papers” en las que se pedían experimentos adicionales y también por cuidar de mis ratoncitos. A Elvira, por ser una excepcional compañera de laboratorio y amiga, siempre con una sonrisa en la cara y optimista ante cualquier adversidad, a pesar de estar en una situación similar a la que yo viví antes de llegar al CIBIR. También me gustaría agradecer su apoyo y tiempo invertido en algunos de los experimentos presentados en esta tesis, así como por la atención mostrada siempre que le explicaba alguna de las técnicas en las que me había formado. Espero y deseo que tengas mucha suerte con tu tesis y poder verte en la presentación de la tuya, en la que seguro mostrarás resultados prometedores. Al resto del personal de la segunda planta del CIBIR: Alfredo, Álvaro, Ana, Angelina, Bea, Bego, Eva, Iñaki, Josune, Juan, Judith, Laura, Luís, María, Rafa, Rodri y Sonia. Por el buen ambiente de trabajo y las risas y tertulias de la hora del café que hacían que me olvidase de los problemas cotidianos. A Angelina, Chus y Lidia, por compartir esta experiencia tan bonita del doctorado y nuestras luchas con el “RAPI”. Os deseo un todo lo mejor para el futuro! A Mikael Adner, mi supervisor durante la estancia predoctoral realizada en el “Instituto Karolinska” de Estocolmo, por su apoyo y consejos como experto en asma para la realización de proyecto colaborativo entre ambas instituciones fruto del cual ha salido una publicación muy bonita. A Josh Gregory, por introducirme en el campo de la alergia durante la estancia predoctoral realizada en el “Instituto Karolinska” de Estocolmo y por su apoyo a nivel técnico. También me gustaría agradecerle que me haya hecho partícipe de la cultura de su país “EE.UU.”, al invitarme a vivir lo que es la “cena de acción de gracias”, un acontecimiento muy importante para él. Por ultimo me gustaría agradecer a mi familia (papa, mama, abuelas y a mi hermano, Rodri), por todo su apoyo a pesar de estar lejos de ellos durante todo este tiempo. A ellos les debo todo lo que soy, los valores que me han enseñado y que me han hecho ser como soy, entre los cuales se encuentran la constancia, la humildad y la generosidad. Durante estos 4 años he tenido momentos muy buenos debido a los buenos resultados obtenidos y a las recientes publicaciones del grupo. Por otro lado, también ha habido momentos muy duros tras la pérdida de mi mejor amigo, mi padre, durante mi segundo año aquí en el CIBIR. Gracias a la ayuda de mis compañeros y familia, he tirado hacia adelante como lo habría hecho él, ya que una de sus grandes lecciones que nunca olvidaré es que nunca hay que rendirse, ya que la constancia es lo que hace conseguir grandes cosas en la vida. Siempre recordaré sus últimas palabras hacia mí, en las que literalmente me dijo “eres auténtico”, palabras que para mí significan mucho porque él creía mucho en mí y en que podía conseguir grandes cosas. Por todo ello, le doy las gracias por lo que me ha enseñado y por ser el mejor padre posible. Me gustaría terminar estos agradecimientos diciendo lo mucho que admiro a mi madre, hermano y abuelas por sacar esa fuerza para tirar hacia adelante en los momentos más duros. LIST OF ABBREVIATIONS 4-OHT 4-hydroxytamoxifen α-SMA Alpha-smooth muscle actin Acta2 Actin, alpha 2, smooth muscle, aorta (gene) Adgre1 Adhesion G protein-coupled receptor E1 (gene) AEC1 Alveolar epithelial type 1 cells AEC2 Alveolar epithelial type 2 cells AHR Airway hyperresponsiveness AKT AKT serine/threonine kinase 1 ALI Acute lung inflammation Aqp5 Aquaporin 5 (gene) AQP5 Aquaporin 5 ARDS Acute respiratory distress syndrome BADJ Bronchioalveolar duct junction BAL Bronchoalveolar lavage BALF Bronchoalveolar lavage fluid BASCs Bronchioalveolar stem cells BLM Bleomycin BM Bone marrow bp Base pairs BSA Bovine serum albumin BSCs Basal stem cells BW Body weight Ccl2 Chemokine (C-C motif) ligand 2 (gene) Ccl5 Chemokine (C-C motif) ligand 5 (gene) Ccl11 Chemokine (C-C motif) ligand 11 (gene) CCL11 C-C motif chemokine ligand 11 (eotaxin-1) Cd209a CD209a antigen (gene) CD3 CD3 molecule Cd4 CD4 antigen (gene) CD4 CD4 molecule cDNA Complementary DNA Cgrp Calcitonin/calcitonin-related polypeptide, alpha (Calca) Col1a1 Collagen, type I, alpha 1 (gene) COPD Chronic obstructive pulmonary disease COX2 Cytochrome c oxidase subunit II Cre Cre recombinase CREBBP CREB binding protein Csf1 Colony stimulating factor 1 (macrophage) (gene) Cxcl1 Chemokine (C-X-C motif) ligand 1 (gene) Derp1 Derp1 allergen DNA Deoxyribonucleic acid ECL Enhanced chemilumisicence EDTA Ethylenediaminetetraacetic acid ELISA Enzyme-linked immunosorbent assay EP300 E1A binding protein p300 F4/80 Adhesion G protein-coupled receptor (ADGRE1) FDR False discovery rate Foxm1 Forkhead box M1 (gene) FOXM1 Forkhead box M1 Foxo1 Forkhead box O1 (gene) FPKM Fragments per kilobase
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