The Effectiveness of Riparian Vegetation at Intercepting Drift from Aerial Pesticide Application

Total Page:16

File Type:pdf, Size:1020Kb

The Effectiveness of Riparian Vegetation at Intercepting Drift from Aerial Pesticide Application Data Report The Effectiveness of Riparian Vegetation at Intercepting Drift from Aerial Pesticide Application A Study by the Washington State Department of Agriculture January 2016 WSDA Publication No. 103-601 (N/1/17) WSDA DATA REPORT: EFFECTIVENESS OF RIPARIAN VEGETATION This page is left blank intentionally 1 WSDA DATA REPORT: EFFECTIVENESS OF RIPARIAN VEGETATION Data Report The Effectiveness of Riparian Vegetation at Intercepting Drift from Aerial Pesticide Application A Study by the Washington State Department of Agriculture By: Gary Bahr1 Matthew Bischof1 Todd Coffey2 Joel Demory1 Margaret Drennan1 Jaclyn Hancock1 George Tuttle1 Kelly McLain1 Abigail Nickelson1 January 2016 WSDA Publication No. 103-601 (N/1/16) Contact Information: George Tuttle Natural Resources Assessment Section Office of the Director [email protected] (360) 902-2066 P.O. Box 42560 Olympia, WA 98502-2560 1 Washington State Department of Agriculture, Natural Resources Assessment Section 2 Washington State University, Department of Mathematics and Statistics 2 WSDA DATA REPORT: EFFECTIVENESS OF RIPARIAN VEGETATION Acknowledgements Matthew Bischof, Jaclyn Hancock, Brian Scott, Joel Demory, Abigail Nickelson, Kelly McLain, George Tuttle, Margaret Drennan, and Rod Baker, WSDA NRAS staff who conducted field work for this project Todd Coffey, PhD, Washington State University, Department of Mathematics and Statistics Whatcom County blueberry producers who participated in this study Aaron Bagwell, Whatcom Farmers Co-Op/CHS Kyle Blackburn, Essential Flight Ops, LLC Tony Hawkes, Scott Hecht, Cathy Laetz, and Thomas Hooper, David Baldwin, NMFS Pesticide BiOp team Washington Blueberry Commission Washington Red Raspberry Commission Steve Thun and Rick Jordan, Pacific Agricultural Laboratory who conducted lab analysis for this project EPA Office of Pesticide Program Environmental Fate and Effects Division staff Michael Grove, EPA Region 10 Bernalyn McGaughey and staff, Compliance Services International Spray Drift Issue Management Team members, Crop Life America Heather Hansen, Washington Friends of Farms and Forests John Hanzas, Stone Environmental Paul Whatling, Cheminova/FMC Scott Jackson, BASF Harold W. Thistle, U.S. Forest Service Tim Bargar, U.S. Geological Survey Vince Hebert, Washington State University Whatcom Conservation District staff 3 WSDA DATA REPORT: EFFECTIVENESS OF RIPARIAN VEGETATION Table of Contents Acknowledgements ....................................................................................................................................... 3 Table of Contents .......................................................................................................................................... 4 List of Figures ............................................................................................................................................... 5 List of Tables ................................................................................................................................................ 5 Summary ....................................................................................................................................................... 6 1. Introduction ............................................................................................................................................... 6 2. Site Selection and Events ........................................................................................................................... 7 3. Field Methods ............................................................................................................................................ 8 3.1. Site Layout .................................................................................................................................... 8 3.2. Instream Habitat Assessment ........................................................................................................ 9 3.3. Vegetation Assessment ................................................................................................................. 9 3.4. Depositional Sampler Set up ....................................................................................................... 10 3.5. Depositional Sampler Retrieval .................................................................................................. 10 3.6. Weather Station Deployment ...................................................................................................... 11 3.7. Water Sampling Methods............................................................................................................ 11 3.8. Field Documentation, Sample Packaging, and Shipping ............................................................ 12 4. Analytical Methods .................................................................................................................................. 12 4.1. Sample Extraction ....................................................................................................................... 12 4.2. Sample Analysis .......................................................................................................................... 12 5. Data Analysis Methods ............................................................................................................................ 12 6. Preliminary Results, Analysis, and Discussion ....................................................................................... 14 6.1. Study Site Physical Characteristics ............................................................................................. 14 6.2. Weather Conditions during Applications .................................................................................... 16 6.3. Water Sample Results ................................................................................................................. 17 6.4. Depositional Sampler Results ..................................................................................................... 19 6.5. Statistical Analysis ...................................................................................................................... 20 7. Conclusions ............................................................................................................................................. 27 8. Recommendations.................................................................................................................................... 27 9. References ............................................................................................................................................... 28 Appendix A: Maps and Depositional Results ............................................................................................. 29 Appendix B: Project Quality Assurance and Quality Control .................................................................... 39 Laboratory and Field Data Quality ............................................................................................................. 39 Method Reporting Limit ............................................................................................................................. 39 Quality Assurance and Quality Control Samples ....................................................................................... 39 4 WSDA DATA REPORT: EFFECTIVENESS OF RIPARIAN VEGETATION Performance Measures ................................................................................................................................ 40 Field Replicate Sample Results................................................................................................................... 41 Field Blank Sample Results ........................................................................................................................ 44 Matrix Spike/Matrix Spike Duplicate Sample Results ............................................................................... 44 Method Blank Sample Results .................................................................................................................... 45 Surrogate Results ........................................................................................................................................ 45 Laboratory Control Sample Results ............................................................................................................ 45 Hold time and Storage Requirements ......................................................................................................... 47 Quality Assurance Summary References .................................................................................................... 48 Appendix C: Field Forms ............................................................................................................................ 49 List of Figures Figure 1. Layout of transects at a two-sided vegetated site .......................................................................... 9 Figure 2. Depositional sampler stand and assembly ................................................................................... 10 Figure 3. Site geometry and angles used to calculate wind components perpendicular to stream ............. 13 Figure 4. Mean estimates of malathion deposition at control and vegetated sites (EBLUEs) .................... 21 Figure 5. Percent reduction from field-edge (F) to water (W) for all sites, calculated from EBLUPs ....... 22 Figure 6.
Recommended publications
  • Mass Spectrometer Business Presentation Materials
    Mass Spectrometer Business Presentation Materials Hiroto Itoi, Corporate Officer Deputy General Manager of the Analytical & Measuring Instruments Division Shimadzu Corporation Jul. 3, 2018 Contents I. Introduction • Expansion of Mass Spectrometry ………………………………………………………………… p.3 • History of Shimadzu's Growth in Mass Spectrometry …………………………………………… p.5 II. Overview of Mass Spectrometers • Operating Principle, Demand Trends, and Vendors ……………………………………………… p.9 • Mass Spectra ………………………………………………………………………………………… p.10 • Configuration of Mass Spectrometers …………………………………………………………… p.11 • Ionization …………………………………………………………………………………………… p.12 • Mass Separation …………………………………………………………………………………… p.14 III. Shimadzu's Mass Spectrometer Business • Product Type ………………………………………………………………………………………… p.17 • Application Software ………………………………………………………………………………… p.18 • Growth Strategy for Mass Spectrometer Business ……………………………………………… p.19 • Expand/Improve Product Lines …………………………………………………………………… p.20 • Measures to Expand Application Fields …………………………………………………………… p.24 • Measures to Automate Data Processing Using AI ……………………………………………… p.25 IV. Summary • Future Direction ……………………………………………………………………………………… p.26 July 2018 Mass Spectrometer Business Presentation Materials 2 I. Introduction Expansion of Mass Spectrometry (1) Why Mass Spectrometry? Mass spectrometry is able to analyze a wide variety of compounds with high accuracy and high efficiency (simultaneous multicomponent analysis). It offers superior characteristics that are especially beneficial in the following fields,
    [Show full text]
  • Contents • Introduction to Proteomics and Mass Spectrometry
    Contents • Introduction to Proteomics and Mass spectrometry - What we need to know in our quest to explain life - Fundamental things you need to know about mass spectrometry • Interfaces and ion sources - Electrospray ionization (ESI) - conventional and nanospray - Heated nebulizer atmospheric pressure chemical ionization - Matrix assisted laser desorption • Types of MS analyzers - Magnetic sector - Quadrupole - Time-of-flight - Hybrid - Ion trap In the next step in our quest to explain what is life The human genome project has largely been completed and many other genomes are surrendering to the gene sequencers. However, all this knowledge does not give us the information that is needed to explain how living cells work. To do that, we need to study proteins. In 2002, mass spectrometry has developed to the point where it has the capacity to obtain the "exact" molecular weight of many macromolecules. At the present time, this includes proteins up to 150,000 Da. Proteins of higher molecular weights (up to 500,000 Da) can also be studied by mass spectrometry, but with less accuracy. The paradigm for sequencing of peptides and identification of proteins has changed – because of the availability of the human genome database, peptides can be identified merely by their masses or by partial sequence information, often in minutes, not hours. This new capacity is shifting the emphasis of biomedical research back to the functional aspects of cell biochemistry, the expression of particular sets of genes and their gene products, the proteins of the cell. These are the new goals of the biological scientist: o to know which proteins are expressed in each cell, preferably one cell at a time o to know how these proteins are modified, information that cannot necessarily be deduced from the nucleotide sequence of individual genes.
    [Show full text]
  • Modern Mass Spectrometry
    Modern Mass Spectrometry MacMillan Group Meeting 2005 Sandra Lee Key References: E. Uggerud, S. Petrie, D. K. Bohme, F. Turecek, D. Schröder, H. Schwarz, D. Plattner, T. Wyttenbach, M. T. Bowers, P. B. Armentrout, S. A. Truger, T. Junker, G. Suizdak, Mark Brönstrup. Topics in Current Chemistry: Modern Mass Spectroscopy, pp. 1-302, 225. Springer-Verlag, Berlin, 2003. Current Topics in Organic Chemistry 2003, 15, 1503-1624 1 The Basics of Mass Spectroscopy ! Purpose Mass spectrometers use the difference in mass-to-charge ratio (m/z) of ionized atoms or molecules to separate them. Therefore, mass spectroscopy allows quantitation of atoms or molecules and provides structural information by the identification of distinctive fragmentation patterns. The general operation of a mass spectrometer is: "1. " create gas-phase ions "2. " separate the ions in space or time based on their mass-to-charge ratio "3. " measure the quantity of ions of each mass-to-charge ratio Ionization sources ! Instrumentation Chemical Ionisation (CI) Atmospheric Pressure CI!(APCI) Electron Impact!(EI) Electrospray Ionization!(ESI) SORTING DETECTION IONIZATION OF IONS OF IONS Fast Atom Bombardment (FAB) Field Desorption/Field Ionisation (FD/FI) Matrix Assisted Laser Desorption gaseous mass ion Ionisation!(MALDI) ion source analyzer transducer Thermospray Ionisation (TI) Analyzers quadrupoles vacuum signal Time-of-Flight (TOF) pump processor magnetic sectors 10-5– 10-8 torr Fourier transform and quadrupole ion traps inlet Detectors mass electron multiplier spectrum Faraday cup Ionization Sources: Classical Methods ! Electron Impact Ionization A beam of electrons passes through a gas-phase sample and collides with neutral analyte molcules (M) to produce a positively charged ion or a fragment ion.
    [Show full text]
  • Methods of Ion Generation
    Chem. Rev. 2001, 101, 361−375 361 Methods of Ion Generation Marvin L. Vestal PE Biosystems, Framingham, Massachusetts 01701 Received May 24, 2000 Contents I. Introduction 361 II. Atomic Ions 362 A. Thermal Ionization 362 B. Spark Source 362 C. Plasma Sources 362 D. Glow Discharge 362 E. Inductively Coupled Plasma (ICP) 363 III. Molecular Ions from Volatile Samples. 364 A. Electron Ionization (EI) 364 B. Chemical Ionization (CI) 365 C. Photoionization (PI) 367 D. Field Ionization (FI) 367 IV. Molecular Ions from Nonvolatile Samples 367 Marvin L. Vestal received his B.S. and M.S. degrees, 1958 and 1960, A. Spray Techniques 367 respectively, in Engineering Sciences from Purdue Univesity, Layfayette, IN. In 1975 he received his Ph.D. degree in Chemical Physics from the B. Electrospray 367 University of Utah, Salt Lake City. From 1958 to 1960 he was a Scientist C. Desorption from Surfaces 369 at Johnston Laboratories, Inc., in Layfayette, IN. From 1960 to 1967 he D. High-Energy Particle Impact 369 became Senior Scientist at Johnston Laboratories, Inc., in Baltimore, MD. E. Low-Energy Particle Impact 370 From 1960 to 1962 he was a Graduate Student in the Department of Physics at John Hopkins University. From 1967 to 1970 he was Vice F. Low-Energy Impact with Liquid Surfaces 371 President at Scientific Research Instruments, Corp. in Baltimore, MD. From G. Flow FAB 371 1970 to 1975 he was a Graduate Student and Research Instructor at the H. Laser Ionization−MALDI 371 University of Utah, Salt Lake City. From 1976 to 1981 he became I.
    [Show full text]
  • A Novel High Resolution Accurate Mass Orbitrap-Based GC-MS
    ROUTINE OR RESEARCH GC-Orbitrap for Environmental Analysis a collaboration between ROUTINE OR RESEARCH GC-Orbitrap for Environmental Analysis Foreword A Novel High Resolution Accurate Mass Orbitrap-based GC-MS Platform for Routine Analysis of Short Chained Chlorinated Paraffins In this study, the performance of a novel bench top, high resolution accurate mass Orbitrap™-based GC-MS was tested for the analysis of SCCPs. System performance was tested using full-scan acquisition and simple instrumental setup. Pyrolysis-GC-Orbitrap MS - A Powerful Analytical Tool for Identification and Quantification of Microplastics in a Biological Matrix The purpose of the experiments described in this work was to assess the applicability of pyrolysis-gas chromatography-Orbitrap™ mass spectrometry for the qualitative and quantitative analysis of plastic polymers in complex biological matrices. Low Level Quantification of NDMA and Non-targeted Contaminants Screening in Drinking Water using GC Orbitrap Mass Spectrometry In this work, a sensitive and selective method for NDMA detection and quantification using high resolution accurate mass GC Orbitrap™ technology is described. Overcoming Analytical Challenges for Polybrominated Diphenyl Ethers (PBDEs) Analysis in Environmental Samples using Gas Chromatography – Orbitrap Mass Spectrometry The note demonstrates the quantitative performance of the Thermo Scientific™ Exactive™ GC Orbitrap™ GC-MS mass spectrometer for the analysis of polybrominated diphenyl ethers (PBDEs) in environmental samples. Versatility of GC-Orbitrap Mass Spectrometry for the Ultra-trace Detection of Persistent Organic Pollutants in Penguin Blood from Antarctica In this study, the performance of the Thermo Scientific™ Q Exactive™ GC Orbitrap™ mass spectrometer was evaluated for routine analysis of POPs within King penguin blood from Antarctica.
    [Show full text]
  • Thermospray Mass Spectrometry , a New Technique for Studying The
    430 Notizen Thermospray Mass Spectrometry , a New charged droplets. These droplets decompose in a Technique for Studying the Relative Stability heated jet chamber in which a pressure of several of Cluster Ions of Salts mbar is maintained, and desolvated ions enter the mass analyser via a small orifice. Typically quadru- G. Schmelzeisen-Redeker, S. S. Wong, pole mass filters are employed. For the production U. Giessmann, and F. W. Röllgen of salt cluster ions the temperatures of the jet and in Institut für Physikalische Chemie der Universität Bonn the jet chamber must be high enough to allow the Z. Naturforsch. 40a,430-431 (1985); decomposition of charged small solid particles. For received February 23. 1985 the ammonium halide this condition is easily achieved. Thermospray (TSP) mass spectrometry provides a means of studying stability effects in the frequency distribution of The ammonium chloride cluster ion distribution cluster ions of salts under conditions of heat transfer to the as obtained by the new TSP technique is shown in cluster ions via thermal collisions in a gas. This is demon- strated for the cluster ions of ammonium chloride. The Figure 2. The TSP mass spectrum was obtained TSP mass spectrum is compared with that obtained by with the experimental arrangement described in sputtering of the salt in secondary ion mass spectrometry. [16], A quadrupole mass filter with a mass range 1-420 was utilized. A 0.1 molar aqueous solution of NH C1 was applied. The temperature at the end Cluster ions of salts are easily generated by 4 of the capillary was 250 °C and the temperature of particle impact in secondary ion mass spectrometry the jet chamber was 180 °C.
    [Show full text]
  • Two New Devices for Identifying Electrochemical Reaction
    Article pubs.acs.org/ac Two New Devices for Identifying Electrochemical Reaction Intermediates with Desorption Electrospray Ionization Mass Spectrometry † ‡ † † Heyong Cheng, , Xin Yan, and Richard N. Zare*, † Department of Chemistry, Stanford University, Stanford, California 94305-5080, United States ‡ College of Material Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou, 310036, China *S Supporting Information ABSTRACT: Desorption electrospray ionization mass spectrometry (DESI- MS) previously has been used to capture and identify transient intermediates in electrochemical redox reactions on a platinum-covered rotating waterwheel. We present here two different setups that use a flat surface with porous carbon tape as the working electrode, where analyte-containing microdroplets from the DESI probe contacted with electrolyte supplied onto the surface. One setup had the conducting carbon tape in the form of a grooved inclined plane; the other one was in the form of a flat plane that had the conducting carbon tape as its front surface. Both these setups, which were relatively robust and easy to operate, overcame interference from the electrospray sheath gas that disturbs and dries the flowing electrolyte. By using the inclined-plane device, we observed radical cations and dimer species generated in the electrochemical oxidation of triphenylamine, diimine and imine alcohol in the electrochemical oxidation of uric acid, and the reductive cleavage of disulfide bonds in glutathione disulfide. By using the device with the flat carbon tape, we detected nitrenium ions generated in the electrochemical oxidation of N,N′-dimethyoxydiphenylamine and di-p-tolylamine. Our experience suggests that the flat porous carbon tape surface might be preferable over the inclined plane because of its ease of setup.
    [Show full text]
  • Accelerated Reaction Kinetics in Microdroplets: Overview and Recent Developments
    PC71CH02_Cooks ARjats.cls April 9, 2020 11:29 Annual Review of Physical Chemistry Accelerated Reaction Kinetics in Microdroplets: Overview and Recent Developments Zhenwei Wei,1 Yangjie Li,1 R. Graham Cooks,1 and Xin Yan2 1Department of Chemistry, Purdue University, West Lafayette, Indiana 47907, USA; email: [email protected] 2Department of Chemistry, Texas A&M University, College Station, Texas 77842, USA; email: [email protected] Annu. Rev. Phys. Chem. 2020. 71:31–51 Keywords The Annual Review of Physical Chemistry is online at reaction acceleration, interfacial reactions, ion solvation, electrospray Annu. Rev. Phys. Chem. 2020.71:31-51. Downloaded from www.annualreviews.org physchem.annualreviews.org Access provided by Radboud Universiteit Nijmegen on 04/15/21. For personal use only. ionization, mass spectrometry https://doi.org/10.1146/annurev-physchem-121319- 110654 Abstract Copyright © 2020 by Annual Reviews. Various organic reactions, including important synthetic reactions involving All rights reserved C–C, C–N, and C–O bond formation as well as reactions of biomolecules, are accelerated when the reagents are present in sprayed or levitated micro- droplets or in thin films. The reaction rates increase by orders of magnitude with decreasing droplet size or film thickness. The effect is associated with reactions at the solution–air interface. A key factor is partial solvation of the reagents at the interface, which reduces the critical energy for reaction. This phenomenon is of intrinsic interest and potentially of practical value as a simple, rapid method of performing small-scale synthesis. 31 PC71CH02_Cooks ARjats.cls April 9, 2020 11:29 1. INTRODUCTION: BACKGROUND AND HISTORY Mass spectrometry (MS), the art of ionization of compounds and manipulation of the resulting ions in vacuum, is widely used to measure the mass-to-charge ratios (m/z) of ionized compounds for their chemical characterization (1).
    [Show full text]
  • Influence of Postharvest Handling on the Concentration of Pesticide
    POSTHARVEST BIOLOGY & TECHNOLOGY HORTSCIENCE 37(3):554–558. 2002. from the early (‘Juneprince’), middle (‘Har- vester’), late (‘Flameprince’ and ‘O’Henry’) and very late (‘Parade’) harvest seasons, fairly Influence of Postharvest Handling on represent the full range of pesticide use pat- terns. Three different grower operations were the Concentration of Pesticide sampled based on the differences in field op- erations among those operations (Table 1). Residues in Peach Peel Farm A and C sprayed methyl parathion from every row middle at a lower rate than Farm B Kathryn C. Taylor1 where methyl parathion was sprayed in alter- nate row middles. Farm C was located in an Horticulture Department, University of Georgia, 21 Dunbar Road, Byron, area with slightly different insect and disease GA 31008 pressures, resulting in somewhat reduced ap- plication rates and frequencies. Parshall B. Bush 1998 Trial. ‘Flameprince’ and ‘O’Henry’ Agricultural Services Laboratory, University of Georgia, 2300 College peel samples, taken before and after packing Station Road, Athens, GA 32602 house process, were analyzed for methyl par- athion, fenbuconazole, propiconazole, cap- Additional index words. captan, carbaryl, methyl parathion, packing line, phosmet, tan and carbaryl. Farm records indicated that propiconazole, Prunus persica the following spray rates were applied by an airblast sprayer at 935 L·ha–1: 1.17 L L·ha–1 Abstract. To discern how the packing process influences pesticide residue loads on peach methyl parathion (Penncap M®; Elf Atochem, (Prunus persica L. Batsch) fruit; postharvest, post hydrocooled, and post brushed fruit Philadelphia) on 8 Apr. 1998, 15 May 1998, were assessed for levels of several pesticides.
    [Show full text]
  • Discovery and Quantitation of Protein Modifications Using Targeted Mass
    Discovery and Quantitation of Protein Modifications using Targeted Mass Spectrometry Dissertation Presented in Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy in the Graduate School of The Ohio State University By Jia You, M.E. Graduate Program in Chemistry The Ohio State University 2012 Dissertation Committee: Dr. Michael A. Freitas, Advisor Dr. Dehua Pei, Co-Advisor Dr. Anne Co Copyright by Jia You 2012 Abstract In this dissertation, efforts were focused on the development of targeted proteomic assays to elucidate differences in protein profiles present in diseases and their correlation with other molecular markers (proteins or microRNA). In Chapter 2, a high-sensitivity TFA-free LC-MS method is described. The analysis of proteins by reversed-phase liquid chromatography (RPLC) commonly involves the use of TFA as an ion-pairing agent, even though it forms adducts and suppresses sensitivity. The presence of adducts can complicate protein molecular weight assignment especially when protein isoforms coelute as in the case of histones. To mitigate the complicating effects of TFA adducts in protein LC-MS, TFA-free methods for protein separation optimized. Protein standards and histones were used to evaluate TFA-free separations using capillary (0.3 mm id) and nanoscale (0.1 mm id) C8 columns with the ion-pairing agents, formic acid or acetic acid. The optimized method was then used to examine the applicability of the approach for histone characterization in human cancer cell lines and primary tumor cells from chronic lymphocytic leukemia patients. ii In chapter 3, a targeted mass spectrometry approach was used to examine nitration and nitrosylation of tyrosine residues on tropomyosin.
    [Show full text]
  • Atmospheric Pressure Chemical Ionization (APCI): Less Polar
    LC-MS Based Metabolomics AnalysingAnalysing thethe METABOLOMEMETABOLOME 1.1. MetaboliteMetabolite ExtractionExtraction 2.2. MetaboliteMetabolite detectiondetection (with(with oror withoutwithout separation)separation) 3.3. DataData analysisanalysis MetaboliteMetabolite DetectionDetection GC-MS: Naturally volatile or made volatile (any organic- flavors, sugars, lipids, acids) NMR – any compound containing hydrogen HP Liquid - Chromatography + detector Comon detectors- - UV-detector (phenolics) - MASS SPECTROMETER (MS) as detector (LC-MS) MetaboliteMetabolite DetectionDetection MASS SPECTROMETER (MS) as detector (LC-MS): Compounds that are not well characterized by other methods: Non volatile High molecular weight Too sensitive to heat to be analyzed by GC Your Result Sample LC/MS Sample Sample Efficient Separation Preparation Introduction Gradient (column) Data (computer) LC- MS Ions Ions Ionization UV Detection Separation MS Interface Spectra Today ComponentsComponents inin LCLC--MSMS Ion Ion Formation Sorting PeptiPeptiddee && ProteinProtein SeqSequuenciencinngg Compound ID APcI Analyzer Software LC Structure Elucidation ESI & Detector Quantitation Interface: Chemical Triple Quadrupole Atmospheric Results Separation Quadrupole -Time Of Flight Pressure Quadrupole - Ion-Trap Ionization FT-MS MassMass SpectrometerSpectrometer 1. Breaks up constituents into molecular ions and other fragments 2. The ions then pass through an electric and/or magnetic field that separates them according to their mass-to-charge ratio (m/z) 3. Measures masses MassMass SpectrometerSpectrometer 4. Universal detection method * compared to UV/VIS (PDA), fluorescence etc. * more specific than NMR 5. More sensitive for most compounds 6. Structural information on metabolite * fragmentation pattern * accurate mass 7. For both LC and GC TechnologyTechnology ofof LCLC--MSMS andand LCLC--MSMS--MSMS –– InterfacesInterfaces-- IonizationIonization (elimination(elimination ofof solventsolvent andand generationgeneration ofof gasgas--phasephase ions)ions) –– e.g.e.g.
    [Show full text]
  • Liquid Chromatography-Mass Spectrometry Interface for Detection of Extraterrestrial Organics
    LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY INTERFACE FOR DETECTION OF EXTRATERRESTRIAL ORGANICS Adrian E. Southard Stephanie A. Getty, Manuel J.Ferrance Universities Space Research Balvin, Jamie E. Elsila, Ana J2F Engineering, Charlottesville, Association, Espiritu Melina Carl VA 22911 USA 8800 Greenbelt Rd. Kotecki, Deborah W. Greenbelt, MD 20771 USA. Towner, J.P. Dworkin, 301-286-4457 Daniel P. Glavin, and Paul [email protected] R. Mahaffy NASA Goddard Space Flight Center 8800 Greenbelt Rd. Greenbelt, MD 20771 USA Abstract—The OASIS (Organics Analyzer for Sampling Icy mass spectrometry (LC-MS) [1]. Future high-priority surfaces) microchip enables electrospray or thermospray of planetary exploration will target destinations that are likely analyte for subsequent analysis by the OASIS time-of-flight mass host to a broad diversity of inorganic and organic spectrometer. Electrospray of buffer solution containing the composition. Primitive bodies, such as comets and nucleobase adenine was performed using the microchip and carbonaceous asteroids, are thought to have contributed an detected by a commercial time-of-flight mass spectrometer. Future testing of thermospray and electrospray capability will be inventory of prebiotic chemistry to the early Earth, and performed using a test fixture and vacuum chamber developed cataloguing their present-day, cryo-trapped organic diversity especially for optimization of ion spray at atmosphere and in low can help to offer more detail about the breadth of organic pressure environments. astrochemistry that is representative of the Solar System’s origins and evolution. Major classes of organics are likely TABLE OF CONTENTS to be present on these primitive, small bodies, including polycyclic aromatics, carboxylic acids, alcohols, aldehydes and ketones, amines, nucleobases, and amino acids.
    [Show full text]