The Evolution of Complex Calls in Meadow
Total Page：16
File Type：pdf, Size：1020Kb
THE EVOLUTION OF COMPLEX CALLS IN MEADOW KATYDIDS _______________________________________ A Dissertation presented to the Faculty of the Graduate School at the University of Missouri-Columbia _______________________________________________________ In Partial Fulfillment of the Requirements for the Degree Doctor of Philosophy _____________________________________________________ by NATHAN HARNESS Dr. Johannes Schul, Dissertation Supervisor July 2018 The undersigned, appointed by the dean of the Graduate School, have examined the dissertation entitled THE EVOLUTION OF COMPLEX CALLS IN MEADOW KATYDIDS presented by Nathan Harness, a candidate for the degree of doctor of philosophy, and hereby certify that, in their opinion, it is worthy of acceptance. Professor Johannes Schul Professor Sarah Bush Professor Lori Eggert Professor Patricia Friedrichsen For my family Rachel and Mayr have given me so much. They show me unselfish affection, endless support, and generosity that seems to only grow. Without them the work here, and the adventure we’ve all three gone on surrounding it, would not have been possible. They have sacrificed birthdays, anniversaries, holidays, and countless weekends and evenings. They’ve happily seen me off to weeks of field work and conference visits. I am thankful to them for being so generous, and completely lacking in resentment at all the things that pull their husband and dad in so many directions. They have both necessarily become adept at melting away anxiety; I will forever be indebted to the hugs of a two-year-old and the kind words of his mom. Rachel and Mayr both deserve far more recognition than is possible here. I also want to thank my parents and brother and sisters. My dad, Ralph, taught me that there is nothing insurmountable, nothing that cannot be solved, no mountain that can’t be moved if I really want it moved. He has given me a lot, but this lesson in unconquerable optimism has meant the most for the trajectory of my life. My mom, Betsy, was my first biology teacher. Both formally, in 9th grade, and informally, as early as I can remember. I can’t thank her enough for lifting the lid on a world of wonder and curiosity. My brother, Mark, and two sisters, Emily and Aubrey, have taught me so much. From playing in our kitchen as kids to watching them interact with and teach and love their own children, they have helped me grow as a person socially, emotionally, and intellectually. All of these people, of course, mean much more to me than I’ve written here. The dynamic experiences we’ve all had, and have, together are the basis of how I see the world. ACKNOWLEDGEMENTS Many people have contributed to this work in a variety of important ways. It is difficult to overstate their influence here. First, the members of the Schul lab (Katy Frederick, Rhett Hartman, Megan Murphy, Gideon Ney, Luesoni Johnson, Anna Scharnagl, Montrai Spikes, Kai Murphy, Cole Steinmetz, and Nat Davis) have been deeply involved in every aspect of this work. Together we have been stuck in sand prairies, hiked through marshes thick with alligator and wild boar, learned R, troubleshot broken microphones, and cleaned thousands of pipette tips. We’ve also taken every opportunity to build days at the lab, office, field, and conference that are unforgettable. I’m forever grateful for their impact on my life and work. Other colleagues including numerous faculty, staff, undergraduate and graduate students at the University of Missouri have had truly outsized roles in all areas of this work, my training, and my personal and professional growth. Open labs and office doors have been my most important resource. I want to, in particular, thank each member of my dissertation committee. Johannes Schul, Sarah Bush, Lori Eggert, and Pat Friedrichsen all gave to me in important ways. They provided feedback and direction on every part of the work here, as well as professional and personal encouragement and advice that will stay with me for the rest of my life. Finally, I want to thank my family for their help academically. They have spent hours listening to me practice talks, outline papers, and think aloud through problems. They have truly facilitated every corner of the research found here. ii TABLE OF CONTENTS Acknowledgements ii List of figures v List of Tables vi Abstract vii Chapter 1. THE FUNCTION OF MULTI-COMPONENT MEADOW KATYDID CALLS IN MALE-MALE INTERACTIONS 1 Introduction 1 Methods 5 Results 9 Discussion 10 References 15 Chapter 2. IDENTIFYING CANDIDATE TRAITS IN THE CALLS OF MULTI-SPECIES ASSEMBLIES OF MEADOW KATYDIDS 31 Introduction 31 Methods 34 Results 36 Discussion 39 References 45 Chapter 3. PHYLOGENTIC SIGNAL IN THE COMPLEX CALLS OF MEADOW KATYDIDS 56 Introduction 56 Methods 58 Results 64 Discussion 66 References 70 iii Chapter 4. MEADOW KATYDIDS AS A TOOL FOR TEACHING BIODIVERSITY AND ENGAGING STUDENTS 81 Introduction 81 Background 82 Materials 83 Procedure 84 Discussion 90 References 92 Vita 97 iv LIST OF FIGURES Chapter 1. THE FUNCTION OF MULTI-COMPONENT MEADOW KATYDID CALLS IN MALE-MALE INTERACTIONS Figure 1 25 Figure 2 26 Figure 3 27 Chapter 2. IDENTIFYING CANDIDATE TRAITS IN THE CALLS OF MULTI-SPECIES ASSEMBLIES OF MEADOW KATYDIDS Figure 1 52 Figure 2 53 Figure 3 54 Figure 4 55 Chapter 3. PHYLOGENTIC SIGNAL IN THE COMPLEX CALLS OF MEADOW KATYDIDS Figure 1 76 Figure 2 77 Figure 3 78 Chapter 4. MEADOW KATYDIDS AS A TOOL FOR TEACHING BIODIVERSITY AND ENGAGING STUDENTS Figure 1 93 Figure 2 94 Figure 3 95 Figure 4 96 v LIST OF TABLES Chapter 1. THE FUNCTION OF MULTI-COMPONENT MEADOW KATYDID CALLS IN MALE-MALE INTERACTIONS Table 1 28 Table 2 29 Chapter 2. PHYLOGENTIC SIGNAL IN THE COMPLEX CALLS OF MEADOW KATYDIDS Table 1 79 Table 2 80 vi THE EVOLUTION OF COMPLEX CALLS IN MEADOW KATYDIDS Nathan Harness Dr. Johannes Schul, Dissertation Supervisor ABSTRACT Meadow Katydids (genera Orchelimum and Conocephalus) are a specious group often are found in habitats with several species within the group living in sympatry. They produce complex calls with two distinct phrases, “buzzing” and “ticking”. These two phrases are organized in a highly diverse way across species. This diversity of call patterns in Meadow Katydids provides an excellent opportunity to comparatively study the evolution of complex calls. We tested the function of the two call phrases in male-male interactions. we examined the structure of the male call in the context of communities to identify candidate traits (i.e. traits likely involved in reproductive isolation). We constructed a molecular phylogeny from twenty species of Meadow Katydids, and examined the phylogenetic signal within call traits. The results of all of this taken together suggests ticking evolved in the context of male-male interaction, buzzing has been important for diversification, and in some species females have co-opted the tick to also function in reproductive isolation. Importantly, we have also designed and field-tested a plan to use Meadow Katydids as tools in primary, secondary, and post-secondary classrooms/laboratories. vii Chapter 1 THE FUNCTION OF MULTI-COMPONENT MEADOW KATYDID CALLS IN MALE-MALE INTERACTIONS Introduction Acoustic communication functions mainly in social contexts, particularly for attracting females for reproduction, and in male-male interactions (review in Gerhardt and Huber 2002). Insects and anurans encode the information relevant to these contexts in a multitude of signal components. The diversity of these components might, or might not, reflect their potential roles in these complex social situations (Greenfield 2002). Advertisement calls, often highly stereotyped, function primarily in male-female communication. However, they also regularly play a role in male-male interactions. In crickets, for example, males show phonotaxis to other males' advertisement calls. This double function of the advertisement call is widespread, occurring in frogs (Sullivan and Wagner 1988, Wagner 1989, Rand and Ryan 1981, Ayre et al.,1984), toads (Harrison and Littlejohn 1985, Gerhardt 1994, Sullivan and Leek 1986), and crickets (Alexander 1961). In some groups, advertisement calls are even the only signal produced (e.g. Tettigoniidae, Shaw et al. 1982). 1 In many insect and frog groups, male-male interactions involve discrete signals or signal components intended primarily for other males. For example, in some systems aggressive calls are produced if other males approach (Wells & Schwartz 1984, Schwartz 1989, Adamo and Hoy 1995), or as part of elaborate dominance interactions (Simmons 1986, Littlejohn et al.,1985, Lopez et al.,1988, Schwartz 1989, Brenowitz and Rose 1999). In species with complex advertisement calls, specific signal components may function predominantly in male-male interactions. In the Coqui frogs’ two-phrase ‘Co-Qui’ call, the first phrase ‘Co’ is used in male-male territorial encounters with the ‘Qui’ phrase being directed at females (Narins and Capranica 1978). Ligurotettix planum grasshoppers produce a two-phrase call, with one phrase ('rasps') used in territorial encounters and the other ('shucks') in attracting females (Greenfield and Minckley 1993). These discrete call components intended for males may be co-opted into the male-female advertisement call, either increasing the attractiveness or even being necessary to elicit female responses (Berglund et al.,1996, Borgia 2006, Borgia and Coleman 2000, Greenfield 2002). Tungara frogs, for instance, have two distinct phrases. One phrase is necessary and sufficient for female phonotaxis, and the other enhances attractiveness to the call (Rand and Ryan 1981). Fisher (1930) suggested that complex signals like these can evolve by co-opting components from different behavioral contexts such as male-male interactions (War Propaganda Model). Ultimately, to reconstruct the evolutionary 2 history of these complex calls, elucidating the function of different call components is necessary (Laidre 2012). Within katydids, (Orthoptera, Tettigoniidae), males typically produce only one simple call that is intended for both males and females (Gwynne 2001).