DOCSLIB.ORG

Proceedings 2002 Proceedings 2002

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text

Load more

XXVIII International Conference on Animal Genetics in Göttingen - Germany ISAG International Society for Animal Genetics Proceedings 2002 ISBN 3-00-010597-2 28th ISAG Conference 2002 Contents Invitation of Local Organizing Committee ................................................................... 2 Greeting from the Minister for Science and Culture .................................................... 3 Members of Local Organizing Committee................................................................... 4 Presentation of Guest Speakers ................................................................................. 5 Abstracts: Section A: Genome technologies................................................................. 26 Section B: MHC and Immunogenetic ........................................................... 41 Section C: Functional Genomics ................................................................. 47 Section D: Marker, Polymorphism and Biodiversity...................................... 78 Section E: Associations between markers and traits.................................. 147 Section F: Bioinformatics............................................................................ 178 Index of Authors...................................................................................................... 184 These proceedings are published under: ISBN 3-00-010597-2 1 28th ISAG Conference 2002 Georg-August-University City of Göttingen Dear colleagues, on behalf of the Local Organizing Committee, the International Society for Animal Genetics and the Georg-August-University of Göttingen, we would like to welcome all delegates and accompanying persons to the 28th International Conference on Animal Genetics in Göttingen. It is a great pleasure and honor for us that a conference of the ISAG will take place again in Göttingen after 17 years. The conference will be a unique blending of individual sessions and themes designed to provide you with an exceptional experience. The conference gathers a cadre of international experts in molecular genetics, molecular biology and related fields. These world-class presenters bring together a wealth of experience and knowledge for your benefit. Add to this the ability to share ideas and interact with a global network as well as to view the latest results in Animal Genetics from around the world and you have a conference that cannot be missed. The ideal forum to increase your personal knowledge and professional capabilities, the ISAG2002 conference will sure exceed your expectations. We wish you all pleasant, inspiring and motivating days in one of the oldest and most attractive University cities in Germany. Because of its central geographical position in Germany, Göttingen is also an extremely good starting point for pre- and post conference tours. We hope to see you in Göttingen at the International Conference and look forward to welcoming you to the "City of Science". Bertram Brenig Jan-Nikolaus Meyer 2 28th ISAG Conference 2002 Greeting from the Minister for Science and Culture of Lower Saxony, Thomas Oppermann Only 17 years ago the term Animal Genetics was in public at most connected with the attempt to increase the milk production through breeding. Today it can be found in almost any news media, related to a variety of issues. “Dolly”, the name of the first ever sheep that had been cloned is just as well the label for a milestone in the history of modern science. For weeks on end “Dolly” made headlines and triggered controversial discussions about genetic engineering and biological sciences. But another problem just as well represents the new requirements Animal Genetics is facing today: The public world-wide was alarmed when BSE spread among the cattle all over Europe. Suddenly, everybody was looking at this field of veterinary medicine, at Animal Genetics, as a source of hope and wishes as well as fear and worries. Considering this growth in importance, I am especially happy the 28th International Conference for Animal Genetics is taking place in Göttingen again after 17 years. Surely triggered by Animal Genetics, genetics has undergone quite an astonishing development during this time and is becoming one of the most important science branches in the 21st century. The genetic mapping of animals has progressed so far that in the years to come there could be enormous breakthroughs concerning the resistance to illnesses in animals and breeds. As well, the existing methods of animal breeding are going to change drastically due to the progress in Animal Genetics. The 28th International Conference for Animal Genetics is approaching the complexity of these issues from several sides. I am especially pleased that in addition to the participation of several veterinary and biological branches of science, bioethical aspects will be discussed as well. The general public will be expecting the new biological sciences to put up a critical reflection on this issue in particular. I’d like to thank the International Society for Animal Genetics (ISAG) for being host to this conference and the Institute of Veterinary Medicine and the Institute of Animal Breeding and Genetics of the Georg-August-Universität Göttingen and the Chairmen of the local organizing committee, Professor B. Brenig and Dr. Jan Meyer for preparing it and I wish all participants of this conference good progress and stimulating discussions. 3 28th ISAG Conference 2002 Local Organizing Committee 2002 Sabine Bramsmann Andreas Müller-Belecke Bertram Brenig Ina Pfeiffer Claus-Peter Czerny Henner Simianer Gabriele Hörstgen-Schwark Helge Täubert Christoph Knorr Clemens Wollny Ute Margan Secretary: Kaarina Hillmer Jan-N. Meyer Katharina Kurth Burchhard Möllers Treasurer: Monika Werst Institute of Animal Breeding and Genetics, Albrecht-Thaer-Weg 3 D-37075 Göttingen, Germany Institute of Veterinary Medicine, Groner Landstraße 2, D-37073 Göttingen, Germany Göttingens most famous monument „Gänseliesel“ 4 28th ISAG Conference 2002 Gue t ea ers Topic Prof. Dr. Adriano Aguzzi (Switzerland) The immunobiology of prion [email protected] diseases Prof. Dr. Choy Leong Hew (Singapore) Biotechnology and Transgenesis [email protected] in fish Dr. Bjorn Ingemarsson, PhD (Sweden) Comprehensive DNA analysis T [email protected] using Pyrosequencing M techno Prof Dr. Dr. Bernhard Irrgang (Getmany) Ethical Issues of Genetic [email protected] ManipuJation of Lifestock Prof Dr. Joan Lunney (USA) Can we use genomics to select for [email protected] healthier swine? Prof. Dr. Paula Schneider (Brazil) Biodiversity and Conservation in [email protected] Amazon Prof. Dr. Hans-J. Thiesen (Gennany) Proteornics hans-;[email protected] Dr. Alain Vignal (France) SNP teclmology [email protected] 8 28th ISAG Conference 2002 Adriano Aguzzi studied medicine at the University of Freiburg (Gennany) fonn 1980-1986. He received his Ph.D. in 1986. From 1986 to 1989 he was resident in Neuropathology at the University Hospital of ZUrich (Switzerland). Since 1997 he is full professor of Neuropathology and Director of the Institute of Neuropathology at the University of ZUrich. He is president of the Swiss Society of Neuropathology and member of severa] international Societies. His research interests are in the area of prion diseases with special focus on their pathogenesis. Choy Leong Hew is Head of the Department of Biological Sciences at the University of Singapore. He received his Ph.D. in 1970 at the University of British Columbia. From 1972 to 1974 he was a C.H. Best fellow at the Banting & Best Dept. of Medical Research of the University of Toronto (Canada). He was then working at the Memorial University of ~ Newfoundland as assistant professor until 1983. From 1983 to 1999 he was a visting professor at a variety of Universities in Canada and China. His main research areas are biology and biotechnology of antifreeze proteins, transgenic fish, molecular endocrinology and proteomics. Bjorn Ingemarsson is Director of Pyrosequencing AB's technical and scientific support function based m Uppsala. Prior to Jommg Pyrosequencing 3 years ago, he worked as a specialist in automated DNA analysis at Amersharn Pharmacia Biotech. He aquired his Ph.D. in plant physiology at the Stockholm University, where he thereafter continued research on the physiology, biochemistry and molecular biology of nitrogen assimilation in plants for several years. Bernhard Irrgang studied Philosophy, Theology and German Philology at the University of Wiirzburg (Germany), Passau (Germany) and Munich (Germany). He received his Ph.D. in Philosophy in 1982 and Theology in 1991. From 1982 to 1992 he worked at different Universities in Germany. Currently, he is Director of the Institute for Philosophy and the Centre for Interdisciplinary Technical Research at the Technical University of Dresden (Gennany). Bernhard Irrgang main interests are in the area of medical and technical ethics, consequences of technology and philosophy of technology. 9 Joan K. Lunney studied Chemistry at the JOM Hopkins University (Baltimore, USA) and received her Ph.D. on studies on the regulation of serum glycoprotein homeostasis in 1976. From 1976 to 1979 she was guest postdoctoral research worker in the Immunology Branch (NCI, NIH, Bethesda, MD). From 1983 until 1995 she was working in different position in the Helminthic Diseases Laboratory at the USDA, ARS (Beltsvi11e, MD). Since 1995 she is GM15 Research Leader and Supervisory Research Immunogeneticist at
Recommended publications
  • Entrez Symbols Name Termid Termdesc 117553 Uba3,Ube1c

    Entrez Symbols Name Termid Termdesc 117553 Uba3,Ube1c

    Entrez Symbols Name TermID TermDesc 117553 Uba3,Ube1c ubiquitin-like modifier activating enzyme 3 GO:0016881 acid-amino acid ligase activity 299002 G2e3,RGD1310263 G2/M-phase specific E3 ubiquitin ligase GO:0016881 acid-amino acid ligase activity 303614 RGD1310067,Smurf2 SMAD specific E3 ubiquitin protein ligase 2 GO:0016881 acid-amino acid ligase activity 308669 Herc2 hect domain and RLD 2 GO:0016881 acid-amino acid ligase activity 309331 Uhrf2 ubiquitin-like with PHD and ring finger domains 2 GO:0016881 acid-amino acid ligase activity 316395 Hecw2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 GO:0016881 acid-amino acid ligase activity 361866 Hace1 HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 GO:0016881 acid-amino acid ligase activity 117029 Ccr5,Ckr5,Cmkbr5 chemokine (C-C motif) receptor 5 GO:0003779 actin binding 117538 Waspip,Wip,Wipf1 WAS/WASL interacting protein family, member 1 GO:0003779 actin binding 117557 TM30nm,Tpm3,Tpm5 tropomyosin 3, gamma GO:0003779 actin binding 24779 MGC93554,Slc4a1 solute carrier family 4 (anion exchanger), member 1 GO:0003779 actin binding 24851 Alpha-tm,Tma2,Tmsa,Tpm1 tropomyosin 1, alpha GO:0003779 actin binding 25132 Myo5b,Myr6 myosin Vb GO:0003779 actin binding 25152 Map1a,Mtap1a microtubule-associated protein 1A GO:0003779 actin binding 25230 Add3 adducin 3 (gamma) GO:0003779 actin binding 25386 AQP-2,Aqp2,MGC156502,aquaporin-2aquaporin 2 (collecting duct) GO:0003779 actin binding 25484 MYR5,Myo1e,Myr3 myosin IE GO:0003779 actin binding 25576 14-3-3e1,MGC93547,Ywhah
  • List of Horse Breeds 1 List of Horse Breeds

    List of Horse Breeds 1 List of Horse Breeds

    List of horse breeds 1 List of horse breeds This page is a list of horse and pony breeds, and also includes terms used to describe types of horse that are not breeds but are commonly mistaken for breeds. While there is no scientifically accepted definition of the term "breed,"[1] a breed is defined generally as having distinct true-breeding characteristics over a number of generations; its members may be called "purebred". In most cases, bloodlines of horse breeds are recorded with a breed registry. However, in horses, the concept is somewhat flexible, as open stud books are created for developing horse breeds that are not yet fully true-breeding. Registries also are considered the authority as to whether a given breed is listed as Light or saddle horse breeds a "horse" or a "pony". There are also a number of "color breed", sport horse, and gaited horse registries for horses with various phenotypes or other traits, which admit any animal fitting a given set of physical characteristics, even if there is little or no evidence of the trait being a true-breeding characteristic. Other recording entities or specialty organizations may recognize horses from multiple breeds, thus, for the purposes of this article, such animals are classified as a "type" rather than a "breed". The breeds and types listed here are those that already have a Wikipedia article. For a more extensive list, see the List of all horse breeds in DAD-IS. Heavy or draft horse breeds For additional information, see horse breed, horse breeding and the individual articles listed below.
  • Tesi Finale Dottorato

    Tesi Finale Dottorato

    INDICE GENERALE 1. INTRODUZIONE............................................................................................................................3 1.1. La tracciabilità dei prodotti di origine animale: alcuni elementi.........................................3 1.2. Elementi di genetica molecolare..........................................................................................4 1.2.1. I marcatori genetici.......................................................................................................4 1.2.2. Lo stato di avanzamento nello studio del genoma degli animali di interesse zootecnico...............................................................................................................................6 1.3. Tracciabilità dei prodotti di origine animale e genetica molecolare....................................8 1.4. I prodotti “monorazza”.......................................................................................................10 1.5. Genetica e biochimica del colore del mantello: alcuni elementi........................................16 1.6. Genetica molecolare e colore del mantello........................................................................19 1.6.1. Il gene MC1R nella specie bovina.............................................................................21 1.6.2. Il gene MC1R nella specie suina................................................................................26 1.6.3. Il gene KIT nella specie bovina..................................................................................26
  • Influence of the Casein Composite Genotype on Milk Quality And

    Influence of the Casein Composite Genotype on Milk Quality And

    animals Article Influence of the Casein Composite Genotype on Milk Quality and Coagulation Properties in the Endangered Agerolese Cattle Breed Sara Albarella 1,* , Maria Selvaggi 2, Emanuele D’Anza 1, Gianfranco Cosenza 3 , Simonetta Caira 4, Andrea Scaloni 4, Annunziata Fontana 5, Vincenzo Peretti 1 and Francesca Ciotola 1 1 Department of Veterinary Medicine and Animal Production, University of Naples Federico II, via Delpino 1, 80137 Naples, Italy; [email protected] (E.D.); [email protected] (V.P.); [email protected] (F.C.); 2 Department of Agricultural and Environmental Science, University of Bari Aldo Moro, 70126 Bari, Italy; [email protected] 3 Department of Agriculture, University of Napoli Federico II, 80055 Portici, Italy; [email protected] 4 Proteomics & Mass Spectrometry Laboratory, ISPAAM, National Research Council, 80147 Naples, Italy; [email protected] (S.C.); [email protected] (A.S.) 5 Laboratory of milk analyses (LSL), Italian Breeders Association (AIA), 00054 Maccarese, Italy; [email protected] * Correspondence: [email protected]; Tel.: +39-081-2536502; Fax: +39-081-292981 Received: 16 April 2020; Accepted: 18 May 2020; Published: 20 May 2020 Simple Summary: Characterization of variants in casein genes allows breeders and researchers to select the most suitable cows for milk production within the same breed. It has been observed that milk from different cattle breeds with the same casein composite genotype shows different chemical and coagulation properties. The aim of this work was to characterize CSN1S1, CSN2 and CSN3 gene variants in Agerolese cattle, an Italian autochthonous breed, milk of which is used to produce “Provolone del Monaco”, a PDO cheese with a relevant economic interest for the Lattari Mountains area and the Sorrento Peninsula (Naples, Italy).
  • Meta-Analysis of Mitochondrial DNA Reveals Several Population

    Meta-Analysis of Mitochondrial DNA Reveals Several Population

    Table S1. Haplogroup distributions represented in Figure 1. N: number of sequences; J: banteng, Bali cattle (Bos javanicus ); G: yak (Bos grunniens ). Other haplogroup codes are as defined previously [1,2], but T combines T, T1’2’3’ and T5 [2] while the T1 count does not include T1a1c1 haplotypes. T1 corresponds to T1a defined by [2] (16050T, 16133C), but 16050C–16133C sequences in populations with a high T1 and a low T frequency were scored as T1 with a 16050C back mutation. Frequencies of I are only given if I1 and I2 have not been differentiated. Average haplogroup percentages were based on balanced representations of breeds. Country, Region Percentages per Haplogroup N Reference Breed(s) T T1 T1c1a1 T2 T3 T4 I1 I2 I J G Europe Russia 58 3.4 96.6 [3] Yaroslavl Istoben Kholmogory Pechora type Red Gorbatov Suksun Yurino Ukrain 18 16.7 72.2 11.1 [3] Ukrainian Whiteheaded Ukrainian Grey Estonia, Byelorussia 12 100 [3] Estonian native Byelorussia Red Finland 31 3.2 96.8 [3] Eastern Finncattle Northern Finncattle Western Finncattle Sweden 38 100.0 [3] Bohus Poll Fjall cattle Ringamala Cattle Swedish Mountain Cattle Swedish Red Polled Swedish Red-and-White Vane Cattle Norway 44 2.3 0.0 0.0 0.0 97.7 [1,4] Blacksided Trondheim Norwegian Telemark Westland Fjord Westland Red Polled Table S1. Cont. Country, Region Percentages per Haplogroup N Reference Breed(s) T T1 T1c1a1 T2 T3 T4 I1 I2 I J G Iceland 12 100.0 [1] Icelandic Denmark 32 100.0 [3] Danish Red (old type) Jutland breed Britain 108 4.2 1.2 94.6 [1,5,6] Angus Galloway Highland Kerry Hereford Jersey White Park Lowland Black-Pied 25 12.0 88.0 [1,4] Holstein-Friesian German Black-Pied C Europe 141 3.5 4.3 92.2 [1,4,7] Simmental Evolene Raetian Grey Swiss Brown Valdostana Pezzata Rossa Tarina Bruna Grey Alpine France 98 1.4 6.6 92.0 [1,4,8] Charolais Limousin Blonde d’Aquitaine Gascon 82.57 Northern Spain 25 4 13.4 [8,9] 1 Albera Alistana Asturia Montana Monchina Pirenaica Pallaresa Rubia Gallega Southern Spain 638 0.1 10.9 3.1 1.9 84.0 [5,8–11] Avileña Berrenda colorado Berrenda negro Cardena Andaluzia Table S1.
  • The Enigmatic Origin of Bovine Mtdna Haplogroup R: Sporadic Interbreeding Or an Independent Event of Bos Primigenius Domestication in Italy?

    The Enigmatic Origin of Bovine Mtdna Haplogroup R: Sporadic Interbreeding Or an Independent Event of Bos Primigenius Domestication in Italy?

    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by PubMed Central The Enigmatic Origin of Bovine mtDNA Haplogroup R: Sporadic Interbreeding or an Independent Event of Bos primigenius Domestication in Italy? Silvia Bonfiglio1, Alessandro Achilli1,2, Anna Olivieri1, Riccardo Negrini3, Licia Colli3, Luigi Liotta4, Paolo Ajmone-Marsan3, Antonio Torroni1, Luca Ferretti1* 1 Dipartimento di Genetica e Microbiologia, Universita` di Pavia, Pavia, Italy, 2 Dipartimento di Biologia Cellulare e Ambientale, Universita` di Perugia, Perugia, Italy, 3 Istituto di Zootecnica, Universita` Cattolica del Sacro Cuore, Piacenza, Italy, 4 Dipartimento di Morfologia, Biochimica, Fisiologia e Produzioni Animali, Universita` di Messina, Messina, Italy Abstract Background: When domestic taurine cattle diffused from the Fertile Crescent, local wild aurochsen (Bos primigenius) were still numerous. Moreover, aurochsen and introduced cattle often coexisted for millennia, thus providing potential conditions not only for spontaneous interbreeding, but also for pastoralists to create secondary domestication centers involving local aurochs populations. Recent mitochondrial genomes analyses revealed that not all modern taurine mtDNAs belong to the shallow macro-haplogroup T of Near Eastern origin, as demonstrated by the detection of three branches (P, Q and R) radiating prior to the T node in the bovine phylogeny. These uncommon haplogroups represent excellent tools to evaluate if sporadic interbreeding or even additional events of cattle domestication occurred. Methodology: The survey of the mitochondrial DNA (mtDNA) control-region variation of 1,747 bovine samples (1,128 new and 619 from previous studies) belonging to 37 European breeds allowed the identification of 16 novel non-T mtDNAs, which after complete genome sequencing were confirmed as members of haplogroups Q and R.
  • The Proteasomal Deubiquitinating Enzyme PSMD14 Regulates Macroautophagy by Controlling Golgi-To-ER Retrograde Transport

    The Proteasomal Deubiquitinating Enzyme PSMD14 Regulates Macroautophagy by Controlling Golgi-To-ER Retrograde Transport

    Supplementary Materials The proteasomal deubiquitinating enzyme PSMD14 regulates macroautophagy by controlling Golgi-to-ER retrograde transport Bustamante HA., et al. Figure S1. siRNA sequences directed against human PSMD14 used for Validation Stage. Figure S2. Primer pairs sequences used for RT-qPCR. Figure S3. The PSMD14 DUB inhibitor CZM increases the Golgi apparatus area. Immunofluorescence microscopy analysis of the Golgi area in parental H4 cells treated for 4 h either with the vehicle (DMSO; Control) or CZM. The Golgi marker GM130 was used to determine the region of interest in each condition. Statistical significance was determined by Student's t-test. Bars represent the mean ± SEM (n =43 cells). ***P <0.001. Figure S4. CZM causes the accumulation of KDELR1-GFP at the Golgi apparatus. HeLa cells expressing KDELR1-GFP were either left untreated or treated with CZM for 30, 60 or 90 min. Cells were fixed and representative confocal images were acquired. Figure S5. Effect of CZM on proteasome activity. Parental H4 cells were treated either with the vehicle (DMSO; Control), CZM or MG132, for 90 min. Protein extracts were used to measure in vitro the Chymotrypsin-like peptidase activity of the proteasome. The enzymatic activity was quantified according to the cleavage of the fluorogenic substrate Suc-LLVY-AMC to AMC, and normalized to that of control cells. The statistical significance was determined by One-Way ANOVA, followed by Tukey’s test. Bars represent the mean ± SD of biological replicates (n=3). **P <0.01; n.s., not significant. Figure S6. Effect of CZM and MG132 on basal macroautophagy. (A) Immunofluorescence microscopy analysis of the subcellular localization of LC3 in parental H4 cells treated with either with the vehicle (DMSO; Control), CZM for 4 h or MG132 for 6 h.
  • 35Th International Society for Animal Genetics Conference 7

    35Th International Society for Animal Genetics Conference 7

    35th INTERNATIONAL SOCIETY FOR ANIMAL GENETICS CONFERENCE 7. 23.16 – 7.27. 2016 Salt Lake City, Utah ABSTRACT BOOK https://www.asas.org/meetings/isag2016 INVITED SPEAKERS S0100 – S0124 https://www.asas.org/meetings/isag2016 epigenetic modifications, such as DNA methylation, and measuring different proteins and cellular metab- INVITED SPEAKERS: FUNCTIONAL olites. These advancements provide unprecedented ANNOTATION OF ANIMAL opportunities to uncover the genetic architecture GENOMES (FAANG) ASAS-ISAG underlying phenotypic variation. In this context, the JOINT SYMPOSIUM main challenge is to decipher the flow of biological information that lies between the genotypes and phe- notypes under study. In other words, the new challenge S0100 Important lessons from complex genomes. is to integrate multiple sources of molecular infor- T. R. Gingeras* (Cold Spring Harbor Laboratory, mation (i.e., multiple layers of omics data to reveal Functional Genomics, Cold Spring Harbor, NY) the causal biological networks that underlie complex traits). It is important to note that knowledge regarding The ~3 billion base pairs of the human DNA rep- causal relationships among genes and phenotypes can resent a storage devise encoding information for be used to predict the behavior of complex systems, as hundreds of thousands of processes that can go on well as optimize management practices and selection within and outside a human cell. This information is strategies. Here, we describe a multi-step procedure revealed in the RNAs that are composed of 12 billion for inferring causal gene-phenotype networks underly- nucleotides, considering the strandedness and allelic ing complex phenotypes integrating multi-omics data. content of each of the diploid copies of the genome.
  • The Myostatin Gene: an Overview of Mechanisms of Action and Its Relevance to Livestock Animals

    The Myostatin Gene: an Overview of Mechanisms of Action and Its Relevance to Livestock Animals

    The Myostatin gene: an overview of mechanisms of action and its relevance to livestock animals Article Accepted Version Aiello, D., Patel, K. and Lasagna, E. (2018) The Myostatin gene: an overview of mechanisms of action and its relevance to livestock animals. Animal Genetics, 49 (6). pp. 505-519. ISSN 1365-2052 doi: https://doi.org/10.1111/age.12696 Available at http://centaur.reading.ac.uk/77388/ It is advisable to refer to the publisher’s version if you intend to cite from the work. See Guidance on citing . To link to this article DOI: http://dx.doi.org/10.1111/age.12696 Publisher: Wiley All outputs in CentAUR are protected by Intellectual Property Rights law, including copyright law. Copyright and IPR is retained by the creators or other copyright holders. Terms and conditions for use of this material are defined in the End User Agreement . www.reading.ac.uk/centaur CentAUR Central Archive at the University of Reading Reading’s research outputs online 1 Review: The Myostatin gene: an overview of mechanisms of action and its 2 relevance to livestock animals 3 D. Aiello 1, K. Patel 2 and E. Lasagna 1 4 5 1 Dipartimento di Scienze Agrarie, Alimentari e Ambientali, Università degli Studi di 6 Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy 7 2 School of Biological Sciences, University of Reading, Berkshire, RG6 6UB, United 8 Kingdom 9 10 Corresponding author: Emiliano Lasagna. Fax: +39 075 5857122. Tel: +39 075 11 5857102. E-mail address: [email protected] 12 13 1 14 15 Summary 16 Myostatin, also known as Growth Differentiation Factor 8, a member of the 17 Transforming Growth Factor-beta (TGF-β) super-family is a negative regulator of 18 muscle development.
  • Play Service 2010 / 2011

    Play Service 2010 / 2011

    Play Service 2010 / 2011 Internationales Theaterinstitut Zentrum Bundesrepublik Deutschland _ _ Inhalt _ _ content Vorbemerkungen..................................................................................................................3 editorial.................................................................................................................................3 Schauspiel Uraufführungen..................................................................................................5 drama world premieres.........................................................................................................5 Schauspiel Erstaufführungen.............................................................................................52 drama debut performances................................................................................................52 Kinder- und Jugendtheater - Uraufführungen....................................................................66 children and youth theatre - world premieres.....................................................................66 Kinder- und Jugendtheater - Erstaufführungen..................................................................84 children and youth theatre - debut performances..............................................................84 Verlage...............................................................................................................................87 publishing houses...............................................................................................................87
  • Short Communication Whole Genome Sequencing Reveals a Large Deletion

    Short Communication Whole Genome Sequencing Reveals a Large Deletion

    Short Communication Whole genome sequencing reveals a large deletion in the MITF gene in horses with white spotted coat colour and increased risk of deafness Jan Henkel1,2, Christa Lafayette3, Samantha A. Brooks4, Katie Martin3, Laura Patterson-Rosa4, Deborah Cook3, Vidhya Jagannathan1,2, Tosso Leeb1,2 1 Institute of Genetics, Vetsuisse Faculty, University of Bern, 3001 Bern, Switzerland 2 DermFocus, University of Bern, 3001 Bern, Switzerland 3 Etalon Inc., Menlo Park, CA 94025, USA 4 Department of Animal Sciences, University of Florida, Gainesville, FL 32611-0910, USA Running title: Equine MITF deletion Address for correspondence Tosso Leeb Institute of Genetics Vetsuisse Faculty University of Bern Bremgartenstrasse 109a 3001 Bern | downloaded: 24.9.2021 Switzerland Phone: +41-31-6312326 Fax: +41-31-6312640 E-mail: [email protected] https://doi.org/10.7892/boris.129241 source: 1 Summary White spotting phenotypes in horses are highly valued in some breeds. They are quite variable and may range from the common white markings up to completely white horses. EDNRB, KIT, MITF, PAX3, and TRPM1 represent known candidate genes for white spotting phenotypes in horses. For the present study, we investigated an American Paint Horse family segregating a phenotype involving white spotting and blue eyes. Six of eight horses with the white-spotting phenotype were deaf. We obtained whole genome sequence data from an affected horse and specifically searched for structural variants in the known candidate genes. This analysis revealed a heterozygous ~63 kb deletion spanning exons 6-9 of the MITF gene (chr16:21,503,211_21,566,617). We confirmed the breakpoints of the deletion by PCR and Sanger sequencing.
  • OXALOACETATO Patent Application Title Inventors

    OXALOACETATO Patent Application Title Inventors

    Method for Extending Lifespan Delaying the Onset of Age-Related Disease: OXALOACETATO Patent application title Inventors: Alan B. Cash (San Diego, CA, US) IPC8 Class: AA61K912FI USPC Class: 424 45 Class name: Drug, bio-affecting and body treating compositions effervescent or pressurized fluid containing organic pressurized fluid Publication date: 2008-11-13 Patent application number: 20080279786 Abstract: A method and composition for extending the lifespan of an individual and delaying the onset of age-related disease is provided. The method includes the administration of an effective dose of oxaloacetate, wherein the oxaloacetate acts to mimic the cellular conditions obtained under caloric restriction to provide similar benefits. The invention further includes methods and compositions for reducing the incidence or treatment of cancer. Compositions and methods for reducing body fat by administering an effective amount of oxaloacetate are likewise provided. Compositions for DNA repair in UV damaged cells is provided are also provided. Similarly, a method for treating a hang-over comprising administering an effective amount of oxaloacetate is disclosed. Claims: 1. Use of an effective lifespan-extending amount of a composition selected from the group consisting of oxaloacetate, oxaloacetic acid, an oxaloacetate salt, alpha-ketoglutarate and aspartate for the manufacture of a medicament for extending the lifespan of an organism. 2. The use of claim 1, wherein said composition is formulated for oral administration. 3. The use of claim 1, wherein said composition is formulated with a buffer. 4. The use of claim 1, wherein said organism is a mammal. 5. The use of claim 4, wherein said mammal is a human.