NAE1 Antibody Cat

Total Page：16

File Type：pdf, Size：1020Kb

NAE1 Antibody Cat. No.: 56-829 NAE1 Antibody NAE1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human HOMOLOGY: Predicted species reactivity based on immunogen sequence: Monkey, Rat This NAE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 172-200 amino acids from the Central region of human NAE1. TESTED APPLICATIONS: IHC-P, WB For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50 September 26, 2021 1 https://www.prosci-inc.com/nae1-antibody-56-829.html PREDICTED MOLECULAR 60 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: NAE1 NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding ALTERNATE NAMES: protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1 ACCESSION NO.: Q13564 PROTEIN GI NO.: 50400302 GENE ID: 8883 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta- amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the BACKGROUND: encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. REFERENCES: 1) Jung, A.C., et al. Int. J. Cancer 126(8):1882-1894(2010) 2) Park, Y., et al. Biochem. Biophys. Res. Commun. 374(2):294-298(2008) September 26, 2021 2 https://www.prosci-inc.com/nae1-antibody-56-829.html 3) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : 4) Li, T., et al. Arch. Biochem. Biophys. 453(1):70-74(2006) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/nae1-antibody-56-829.html.

Copy Link

Recommended publications

Entrez Symbols Name Termid Termdesc 117553 Uba3,Ube1c
Entrez Symbols Name TermID TermDesc 117553 Uba3,Ube1c ubiquitin-like modifier activating enzyme 3 GO:0016881 acid-amino acid ligase activity 299002 G2e3,RGD1310263 G2/M-phase specific E3 ubiquitin ligase GO:0016881 acid-amino acid ligase activity 303614 RGD1310067,Smurf2 SMAD specific E3 ubiquitin protein ligase 2 GO:0016881 acid-amino acid ligase activity 308669 Herc2 hect domain and RLD 2 GO:0016881 acid-amino acid ligase activity 309331 Uhrf2 ubiquitin-like with PHD and ring finger domains 2 GO:0016881 acid-amino acid ligase activity 316395 Hecw2 HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 GO:0016881 acid-amino acid ligase activity 361866 Hace1 HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 GO:0016881 acid-amino acid ligase activity 117029 Ccr5,Ckr5,Cmkbr5 chemokine (C-C motif) receptor 5 GO:0003779 actin binding 117538 Waspip,Wip,Wipf1 WAS/WASL interacting protein family, member 1 GO:0003779 actin binding 117557 TM30nm,Tpm3,Tpm5 tropomyosin 3, gamma GO:0003779 actin binding 24779 MGC93554,Slc4a1 solute carrier family 4 (anion exchanger), member 1 GO:0003779 actin binding 24851 Alpha-tm,Tma2,Tmsa,Tpm1 tropomyosin 1, alpha GO:0003779 actin binding 25132 Myo5b,Myr6 myosin Vb GO:0003779 actin binding 25152 Map1a,Mtap1a microtubule-associated protein 1A GO:0003779 actin binding 25230 Add3 adducin 3 (gamma) GO:0003779 actin binding 25386 AQP-2,Aqp2,MGC156502,aquaporin-2aquaporin 2 (collecting duct) GO:0003779 actin binding 25484 MYR5,Myo1e,Myr3 myosin IE GO:0003779 actin binding 25576 14-3-3e1,MGC93547,Ywhah
[Show full text]
14321 NAE1/APPBP1 (D9I4Z) Rabbit Mab
Revision 1 C 0 2 - t NAE1/APPBP1 (D9I4Z) Rabbit mAb a e r o t S Orders: 877-616-CELL (2355) [email protected] 1 Support: 877-678-TECH (8324) 2 3 Web: [email protected] 4 www.cellsignal.com 1 # 3 Trask Lane Danvers Massachusetts 01923 USA For Research Use Only. Not For Use In Diagnostic Procedures. Applications: Reactivity: Sensitivity: MW (kDa): Source/Isotype: UniProt ID: Entrez-Gene Id: WB H M R Mk Endogenous 60 Rabbit IgG Q13564 8883 Product Usage Information 2. Huang, D.T. et al. (2005) Mol Cell 17, 341-50. 3. Liakopoulos, D. et al. (1998) EMBO J 17, 2208-14. Application Dilution 4. Gong, L. and Yeh, E.T. (1999) J Biol Chem 274, 12036-42. 5. Wada, H. et al. (2000) J Biol Chem 275, 17008-15. Western Blotting 1:1000 6. Sakata, E. et al. (2007) Nat Struct Mol Biol 14, 167-8. 7. Kawakami, T. et al. (2001) EMBO J 20, 4003-12. Storage 8. Podust, V.N. et al. (2000) Proc Natl Acad Sci USA 97, 4579-84. 9. Wu, K. et al. (2002) J Biol Chem 277, 516-27. Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% 10. Amir, R.E. et al. (2002) J Biol Chem 277, 23253-9. glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. 11. Herrmann, J. et al. (2007) Circ Res 100, 1276-91. 12. Walden, H. et al. (2003) Mol Cell 12, 1427-37.
[Show full text]
Is APP Key to the Appbp1 Pathway?
Open Access Full Text Article Austin Alzheimer’s and Parkinson’s Disease A Austin Publishing Group Review Article Cycle on Wheels: Is APP Key to the AppBp1 Pathway? Chen Y1,2*, Neve RN4, Zheng H3, Griffin WST1,2, Barger SW1,2 and Mrak RE5 Abstract 1Department of Geriatrics, University of Arkansas for Alzheimer’s disease (AD) is the gradual loss of the cognitive function due Medical Sciences, USA to neuronal death. Currently no therapy is available to slow down, reverse 2Department of Neurobiology and Developmental or prevent the disease. Here we analyze the existing data in literature and Sciences, University of Arkansas for Medical Sciences, hypothesize that the physiological function of the Amyloid Precursor Protein USA (APP) is activating the AppBp1 pathway and this function is gradually lost during 3Huffington Center on Aging, Baylor College of Medicine, the progression of AD pathogenesis. The AppBp1 pathway, also known as the USA neddylation pathway, activates the small ubiquitin-like protein nedd8, which 4Department of Brain and Cognitive Sciences, covalently modifies and switches on Cullin ubiquitin ligases, which are essential Massachusetts Institute of Technology, USA in the turnover of cell cycle proteins. Here we discuss how APP may activate the 5Department of Pathology, University of Toledo Health AppBp1 pathway, which downregulates cell cycle markers and protects genome Sciences Campus, USA integrity. More investigation of this mechanism-driven hypothesis may provide *Corresponding author: Chen Y, Department insights into disease treatment and prevention strategies. of Geriatrics and Department of Neurobiology and Keywords: APP; Alzheimer’s disease; Ubiquitination; Neddylation; Cell Developmental Sciences, University of Arkansas for cycle Medical Sciences, Little Rock, AR 72205, USA Received: September 02, 2014; Accepted: September 29, 2014; Published: September 30, 2014 Abbreviations at least 18 proteins have been identified to bind AICD [25-27].
[Show full text]
APPBP1 (NAE1) Mouse Monoclonal Antibody [Clone ID: OTI1E10] Product Data
OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA804384 APPBP1 (NAE1) Mouse Monoclonal Antibody [Clone ID: OTI1E10] Product data: Product Type: Primary Antibodies Clone Name: OTI1E10 Applications: IHC, WB Recommended Dilution: WB 1:2000, IHC 1:150 Reactivity: Human, Mouse, Rat Host: Mouse Isotype: IgG1 Clonality: Monoclonal Immunogen: Human recombinant protein fragment corresponding to amino acids 1-274 of human NAE1 (NP_001018170) produced in E.coli. Formulation: PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. Concentration: 1 mg/ml Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G) Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Predicted Protein Size: 50.4 kDa Gene Name: NEDD8 activating enzyme E1 subunit 1 Database Link: NP_001018170 Entrez Gene 84019 RatEntrez Gene 234664 MouseEntrez Gene 8883 Human Q13564 This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 3 APPBP1 (NAE1) Mouse Monoclonal Antibody [Clone ID: OTI1E10] – TA804384 Background: The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein.
[Show full text]
NAE1 (APPBP1) Antibody (C-Term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap7273b
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 NAE1 (APPBP1) Antibody (C-term) Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP7273b Specification NAE1 (APPBP1) Antibody (C-term) - Product Information Application WB, IHC-P,E Primary Accession Q13564 Other Accession Q9Z1A5, Q8VBW6, Q4R3L6, NP_003896 Reactivity Human Predicted Monkey, Mouse, Rat Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Calculated MW 60246 Antigen Region 430-459 NAE1 (APPBP1) Antibody (C-term) - Additional Western blot analysis of anti-APPBP1 Information Antibody (C-term) (Cat.#AP7273b) in mouse brain tissue lysates (35ug/lane). Gene ID 8883 APPBP1(arrow) was detected using the purified Pab. Other Names NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1 Target/Specificity This NAE1 (APPBP1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 430-459 amino acids from the C-terminal region of human NAE1 (APPBP1). Dilution WB~~1:1000 IHC-P~~1:50~100 Western blot analysis of APP-BP1(arrow) using rabbit polyclonal APP-BP1 Antibody Format (C-term) (Cat.#AP7273b). 293 cell lysates (2 Purified monoclonal antibody supplied in ug/lane) either nontransfected (Lane 1) or PBS with 0.09% (W/V) sodium azide. This transiently transfected with the APP-BP1 antibody is purified through a protein G gene (Lane 2) (Origene Technologies). column, followed by dialysis against PBS. Storage Maintain refrigerated at 2-8°C for up to 2 Page 1/2 10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 weeks.
[Show full text]
Recombinant Human NEDD8 E1/APPBP1/UBA3 Protein Catalog Number: ATGP1425
Recombinant human NEDD8 E1/APPBP1/UBA3 protein Catalog Number: ATGP1425 PRODUCT INPORMATION Expression system E.coli Domain 1-463aa UniProt No. Q8TBC4 NCBI Accession No. AAH22853 Alternative Names NEDD8-activating enzyme E1 catalytic subunit, uBE1C PRODUCT SPECIFICATION Molecular Weight 54.4 kDa (487aa) Concentration 1mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT Purity > 90% by SDS-PAGE Tag His-Tag Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description uBA3, also known as NEDD8-activating enzyme E1 catalytic subunit, is the catalytic subunit of the dimeric uBA3- NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-uBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of uBE2M. Recombinant human uBA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. 1 Recombinant human NEDD8 E1/APPBP1/UBA3 protein Catalog Number: ATGP1425 Amino acid Sequence MGSSHHHHHH SSGLVPRGSH MGSHMADGEE PERKRRRIEE LLAEKMAVDG GCGDTGDWEG RWNHVKKFLE RSGPFTHPDF EPSTESLQFL LDTCKVLVIG AGGLGCELLK NLALSGFRQI HVIDMDTIDV SNLNRQFLFR PKDIGRPKAE VAAEFLNDRV PNCNVVPHFN KIQDFNDTFY RQFHIIVCGL DSIIARRWIN GMLISLLNYE DGVLDPSSIV PLIDGGTEGF KGNARVILPG MTACIECTLE LYPPQVNFPM CTIASMPRLP EHCIEYVRML QWPKEQPFGE GVPLDGDDPE HIQWIFQKSL ERASQYNIRG VTYRLTQGVV KRIIPAVAST NAVIAAVCAT EVFKIATSAY IPLNNYLVFN DVDGLYTYTF EAERKENCPA CSQLPQNIQF SPSAKLQEVL DYLTNSASLQ MKSPAITATL EGKNRTLYLQ SVTSIEERTR PNLSKTLKEL GLVDGQELAV ADVTTPQTVL FKLHFTS General References Gong L., et al.
[Show full text]
The Proteasomal Deubiquitinating Enzyme PSMD14 Regulates Macroautophagy by Controlling Golgi-To-ER Retrograde Transport
Supplementary Materials The proteasomal deubiquitinating enzyme PSMD14 regulates macroautophagy by controlling Golgi-to-ER retrograde transport Bustamante HA., et al. Figure S1. siRNA sequences directed against human PSMD14 used for Validation Stage. Figure S2. Primer pairs sequences used for RT-qPCR. Figure S3. The PSMD14 DUB inhibitor CZM increases the Golgi apparatus area. Immunofluorescence microscopy analysis of the Golgi area in parental H4 cells treated for 4 h either with the vehicle (DMSO; Control) or CZM. The Golgi marker GM130 was used to determine the region of interest in each condition. Statistical significance was determined by Student's t-test. Bars represent the mean ± SEM (n =43 cells). ***P <0.001. Figure S4. CZM causes the accumulation of KDELR1-GFP at the Golgi apparatus. HeLa cells expressing KDELR1-GFP were either left untreated or treated with CZM for 30, 60 or 90 min. Cells were fixed and representative confocal images were acquired. Figure S5. Effect of CZM on proteasome activity. Parental H4 cells were treated either with the vehicle (DMSO; Control), CZM or MG132, for 90 min. Protein extracts were used to measure in vitro the Chymotrypsin-like peptidase activity of the proteasome. The enzymatic activity was quantified according to the cleavage of the fluorogenic substrate Suc-LLVY-AMC to AMC, and normalized to that of control cells. The statistical significance was determined by One-Way ANOVA, followed by Tukey’s test. Bars represent the mean ± SD of biological replicates (n=3). **P <0.01; n.s., not significant. Figure S6. Effect of CZM and MG132 on basal macroautophagy. (A) Immunofluorescence microscopy analysis of the subcellular localization of LC3 in parental H4 cells treated with either with the vehicle (DMSO; Control), CZM for 4 h or MG132 for 6 h.
[Show full text]
Drosophila and Human Transcriptomic Data Mining Provides Evidence for Therapeutic
Drosophila and human transcriptomic data mining provides evidence for therapeutic mechanism of pentylenetetrazole in Down syndrome Author Abhay Sharma Institute of Genomics and Integrative Biology Council of Scientific and Industrial Research Delhi University Campus, Mall Road Delhi 110007, India Tel: +91-11-27666156, Fax: +91-11-27662407 Email: [email protected] Nature Precedings : hdl:10101/npre.2010.4330.1 Posted 5 Apr 2010 Running head: Pentylenetetrazole mechanism in Down syndrome 1 Abstract Pentylenetetrazole (PTZ) has recently been found to ameliorate cognitive impairment in rodent models of Down syndrome (DS). The mechanism underlying PTZ’s therapeutic effect is however not clear. Microarray profiling has previously reported differential expression of genes in DS. No mammalian transcriptomic data on PTZ treatment however exists. Nevertheless, a Drosophila model inspired by rodent models of PTZ induced kindling plasticity has recently been described. Microarray profiling has shown PTZ’s downregulatory effect on gene expression in fly heads. In a comparative transcriptomics approach, I have analyzed the available microarray data in order to identify potential mechanism of PTZ action in DS. I find that transcriptomic correlates of chronic PTZ in Drosophila and DS counteract each other. A significant enrichment is observed between PTZ downregulated and DS upregulated genes, and a significant depletion between PTZ downregulated and DS dowwnregulated genes. Further, the common genes in PTZ Nature Precedings : hdl:10101/npre.2010.4330.1 Posted 5 Apr 2010 downregulated and DS upregulated sets show enrichment for MAP kinase pathway. My analysis suggests that downregulation of MAP kinase pathway may mediate therapeutic effect of PTZ in DS. Existing evidence implicating MAP kinase pathway in DS supports this observation.
[Show full text]
Material Data Sheet
MATERIAL DATA SHEET Recombinant Human GST NEDD8 Activating Enzyme (APPBP1/UBA3) Cat. # E312 Neural Precursor Cell Expressed Developmentally Downregulated Gene 8 (NEDD8) Activating Enzyme (APPBP1/UBA3) is a heterodimeric NEDD8activating (E1) enzyme with a predicted molecular weight of 112 kDa. It is responsible for the first step in the conjugation of NEDD8 to protein substrates. The NEDD8 Activating Enzyme heterodimer is composed of a regulatory subunit, Amyloid beta Precursor Protein Binding Protein 1 (APPBP1), and a catalytic subunit, Ubiquitinlike Modifier Activating Enzyme 3 (UBA3). Human APPBP1 is a 534 amino acid (aa) protein with a predicted molecular weight of 60 kDa that is expressed ubiquitously in fetal tissues and in the adult brain (1). APPBP1 is required for UBA3 neddylation activity, regulates enzyme specificity, and is expressed as two isoforms, the full length protein and a second isoform with an alternate Nterminal, aa117, sequence (2). APPBP1 has been shown to drive cell cycle progression, and its expression is increased in the hippocampus of Alzheimer's disease brains (3,4). Human UBA3 is a 463 aa protein with a predicted molecular weight of 52 kDa. It is ubiquitously expressed and shares high aa sequence identity with the Cterminal domain of human UBE1 (5). UBA3 contains an ATPbinding domain and an active site cysteine residue, Cys237 in humans, which are both common to E1 enzymes. Like APPBP1, two isoforms of UBA3 have been identified in humans, the full length protein and a truncated isoform, which lacks aa 821. UBA3 is required for cell cycle progression and has been shown to downregulate steroid receptor activation (4,6).
[Show full text]
A Genome-Wide Rnai Screen Identifies Multiple Synthetic Lethal
A Genome-wide RNAi Screen Identiﬁes Multiple Synthetic Lethal Interactions with the Ras Oncogene Ji Luo,1 Michael J. Emanuele,1 Danan Li,2 Chad J. Creighton,3 Michael R. Schlabach,1 Thomas F. Westbrook,4 Kwok-Kin Wong,2 and Stephen J. Elledge1,* 1Howard Hughes Medical Institute and Department of Genetics, Harvard Medical School, Division of Genetics, Brigham and Women’s Hospital 2Department of Medicine, Harvard Medical School and Department of Medical Oncology, Dana Farber Cancer Center, Ludwig Center at Dana-Farber/Harvard Cancer Center Boston, MA 02115, USA 3Dan L. Duncan Cancer Center Division of Biostatistics 4Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Department of Molecular and Human Genetics, Dan L. Duncan Cancer Center Baylor College of Medicine, Houston, TX 77030, USA *Correspondence: [email protected] DOI 10.1016/j.cell.2009.05.006 SUMMARY (Ngo et al., 2006; Schlabach et al., 2008; Silva et al., 2008). We have developed barcoded, retroviral/lentiviral-based short Oncogenic mutations in the small GTPase Ras are hairpin RNA (shRNA) libraries targeting the entire human genome highly prevalent in cancer, but an understanding of to enable genome-wide loss-of-function analysis through stable the vulnerabilities of these cancers is lacking. We gene knockdown (Silva et al., 2005). Our design also allowed undertook a genome-wide RNAi screen to identify us to use microarray deconvolution to develop a multiplex synthetic lethal interactions with the KRAS onco- screening platform that enables the highly parallel screening gene. We discovered a diverse set of proteins whose of >10,000 shRNAs in a pool-based format (Schlabach et al., 2008; Silva et al., 2008).
[Show full text]
Uncovering New Mechanisms of Cdc34 and Cullin-Ring Activity
UNLV Theses, Dissertations, Professional Papers, and Capstones 12-15-2019 Uncovering New Mechanisms of Cdc34 and Cullin-Ring Activity Spencer Hill Follow this and additional works at: https://digitalscholarship.unlv.edu/thesesdissertations Part of the Biochemistry Commons Repository Citation Hill, Spencer, "Uncovering New Mechanisms of Cdc34 and Cullin-Ring Activity" (2019). UNLV Theses, Dissertations, Professional Papers, and Capstones. 3808. http://dx.doi.org/10.34917/18608668 This Dissertation is protected by copyright and/or related rights. It has been brought to you by Digital Scholarship@UNLV with permission from the rights-holder(s). You are free to use this Dissertation in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s) directly, unless additional rights are indicated by a Creative Commons license in the record and/or on the work itself. This Dissertation has been accepted for inclusion in UNLV Theses, Dissertations, Professional Papers, and Capstones by an authorized administrator of Digital Scholarship@UNLV. For more information, please contact [email protected]. UNCOVERING NEW MECHANISMS OF CDC34 AND CULLIN-RING ACTIVITY By Spencer Wayne Hill Bachelor of Science in Biochemistry University of Nevada, Las Vegas 2013 A dissertation submitted in partial fulfillment of the requirements for the Doctor of Philosophy - Chemistry Department of Chemistry and Biochemistry College of Sciences The Graduate College University of Nevada, Las Vegas December 2019 Dissertation Approval The Graduate College The University of Nevada, Las Vegas November 1st, 2019 This dissertation prepared by Spencer Wayne Hill entitled Uncovering New Mechanisms of Cdc34 and Cullin-Ring Activity is approved in partial fulfillment of the requirements for the degree of Doctor of Philosophy - Chemistry Department of Chemistry and Biochemistry Gary Kleiger, Ph.D.
[Show full text]
OXALOACETATO Patent Application Title Inventors
Method for Extending Lifespan Delaying the Onset of Age-Related Disease: OXALOACETATO Patent application title Inventors: Alan B. Cash (San Diego, CA, US) IPC8 Class: AA61K912FI USPC Class: 424 45 Class name: Drug, bio-affecting and body treating compositions effervescent or pressurized fluid containing organic pressurized fluid Publication date: 2008-11-13 Patent application number: 20080279786 Abstract: A method and composition for extending the lifespan of an individual and delaying the onset of age-related disease is provided. The method includes the administration of an effective dose of oxaloacetate, wherein the oxaloacetate acts to mimic the cellular conditions obtained under caloric restriction to provide similar benefits. The invention further includes methods and compositions for reducing the incidence or treatment of cancer. Compositions and methods for reducing body fat by administering an effective amount of oxaloacetate are likewise provided. Compositions for DNA repair in UV damaged cells is provided are also provided. Similarly, a method for treating a hang-over comprising administering an effective amount of oxaloacetate is disclosed. Claims: 1. Use of an effective lifespan-extending amount of a composition selected from the group consisting of oxaloacetate, oxaloacetic acid, an oxaloacetate salt, alpha-ketoglutarate and aspartate for the manufacture of a medicament for extending the lifespan of an organism. 2. The use of claim 1, wherein said composition is formulated for oral administration. 3. The use of claim 1, wherein said composition is formulated with a buffer. 4. The use of claim 1, wherein said organism is a mammal. 5. The use of claim 4, wherein said mammal is a human.
[Show full text]