NAE1 Antibody Cat. No.: 56-829

NAE1 Antibody

NAE1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Monkey, Rat

This NAE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 172-200 amino acids from the Central region of human NAE1.

TESTED APPLICATIONS: IHC-P, WB

For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50

September 26, 2021 1 https://www.prosci-inc.com/nae1-antibody-56-829.html PREDICTED MOLECULAR 60 kDa WEIGHT:

Properties

This antibody is purified through a A column, followed by peptide affinity PURIFICATION: purification.

CLONALITY: Polyclonal

ISOTYPE: Rabbit Ig

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.

CONCENTRATION: batch dependent

Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: NAE1

NEDD8-activating enzyme E1 regulatory subunit, precursor protein-binding ALTERNATE NAMES: protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1

ACCESSION NO.: Q13564

PROTEIN GI NO.: 50400302

GENE ID: 8883

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The protein encoded by this binds to the beta-amyloid precursor protein. Beta- amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the BACKGROUND: encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a -like protein. This protein is required for progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.

REFERENCES: 1) Jung, A.C., et al. Int. J. Cancer 126(8):1882-1894(2010)

2) Park, Y., et al. Biochem. Biophys. Res. Commun. 374(2):294-298(2008)

September 26, 2021 2 https://www.prosci-inc.com/nae1-antibody-56-829.html 3) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :

4) Li, T., et al. Arch. Biochem. Biophys. 453(1):70-74(2006)

ANTIBODIES FOR RESEARCH USE ONLY.

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September 26, 2021 3 https://www.prosci-inc.com/nae1-antibody-56-829.html