NAE1 Antibody Cat. No.: 56-829 NAE1 Antibody NAE1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Human HOMOLOGY: Predicted species reactivity based on immunogen sequence: Monkey, Rat This NAE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic IMMUNOGEN: peptide between 172-200 amino acids from the Central region of human NAE1. TESTED APPLICATIONS: IHC-P, WB For WB starting dilution is: 1:1000 APPLICATIONS: For IHC-P starting dilution is: 1:10~50 September 26, 2021 1 https://www.prosci-inc.com/nae1-antibody-56-829.html PREDICTED MOLECULAR 60 kDa WEIGHT: Properties This antibody is purified through a protein A column, followed by peptide affinity PURIFICATION: purification. CLONALITY: Polyclonal ISOTYPE: Rabbit Ig CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide. CONCENTRATION: batch dependent Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies STORAGE CONDITIONS: care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: NAE1 NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding ALTERNATE NAMES: protein 1, 59 kDa, APP-BP1, Amyloid protein-binding protein 1, Proto-oncogene protein 1, NAE1, APPBP1 ACCESSION NO.: Q13564 PROTEIN GI NO.: 50400302 GENE ID: 8883 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta- amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the BACKGROUND: encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. REFERENCES: 1) Jung, A.C., et al. Int. J. Cancer 126(8):1882-1894(2010) 2) Park, Y., et al. Biochem. Biophys. Res. Commun. 374(2):294-298(2008) September 26, 2021 2 https://www.prosci-inc.com/nae1-antibody-56-829.html 3) Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) : 4) Li, T., et al. Arch. Biochem. Biophys. 453(1):70-74(2006) ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 26, 2021 3 https://www.prosci-inc.com/nae1-antibody-56-829.html.