Supplementary Table 1. Primers for real time RT-PCR analysis
Gene Forward Primer Reverse Primer ATR GCCGCTCCGATCGTGTAC TTTGTATGCTCTGTGATAACCTTGTTT CASP6 CAGCGCCGAGTGGACTTTT ACAGGGAACCTGCTTCTTTCC CX3CR1 AAGAAAATGAATGCCTTGGTGACT GGAGCACGGGCCAGATT EAF2 CACTGTGCGCTATGACTTCAAAC CACCAACCTCAAGGTATCCTTCA EFNA2 CGGTGGAGGTGAGCATCAAT AGCGGCGCCCCATAGT EIF2K3 CCATACTACAAGAGGGAGAGGAACA TGGGTTGTCGAGGAATCTGACT IHPK2 TCGGCGCCAGCTCTGTAG GCAGGTGGTGTGTGCAAAGT NFATC1 TGGCCCCTATTCCTGTAACG TTCATTTACGTCATCCAGGTACAAC PRKRIR GGATGGACATGAGGCTGATG CCAGCAGTGCCTGAAAGTTATCT RASA2 CCAGCAGCTTGTTGTACACATCA CACAGCTTTGGCCATTTATGAG SH3GLB1 TCCTTCCAACCTCAGTGACCTT TCATAGAGAACCCTGGCCTTTC UBE1DC1 CCGTACCTTTGCCGTAGCAA GTCAGCATTTCAGCAGTCACACT AR GCAGGCAAGAGCACTGAAGATA CCTTTGGTGTAACCTCCCTTGA PSA TGTGCTTCAAGGTATCACGTCAT TGTACAGGGAAGGCCTTTCG β-Actin TGGATCAGCAAGCAGGAGTATG GCATTTGCGGTGGACGAT 18S rRNA CGATGCTCTTAGCTGAGTGT GGTCCAAGAATTTCACCTCT
Supplementary Table 2. Genes Differentially Regulated by AR Knockdown in C4-2 Cells Grown Under Androgen Deprived Conditions
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300005426 5.7 0.0447 BX648957 H300015425 5.4 0.0399 M34715 (PSbetaC/D) H200015569 CLEC4E 5.1 0.0465 AB024718 Ctype lectin, superfamily member 9; macrophageinducible Ctype lectin H300009711 4.9 0.0419 H200003701 EAF2 4.4 0.0434 BC014209 ELL associated factor 2; uncharacterized bone marrow protein BM040 H300003270 LOC284402 4.2 0.0383 AK093495 H300008889 3.8 0.0466 AF255792 H200006579 CX3CR1 3.7 0.0179 BC028078 CX3C chemokine receptor 1 (CX3C CKR1) (CX3CR1) (Fractalkine receptor) (GPR13) H200003948 PLEKHF2 3.6 0.0421 AL834473 phafin 2; PH and FYVE domaincontaining protein 2 H200011349 SAMD13 3.5 0.0407 AK056937 H300015986 EFCAB2 3.4 0.0358 BC005357 H200007324 EFNA2 3.2 0.0279 CN408347 EphrinA2 precursor (EPHrelated receptor tyrosine kinase ligand 6) (LERK6) (HEK7ligand) (HEK7L). H300009564 LOC285941 3.2 0.00748 AK093321 H300005309 TMEM35 3.1 0.0048 BC078658 transmembrane protein 35 H300001392 OR5AS1 3.1 0.00112 AB065543 Olfactory receptor 5AS1. H200014271 UBE2J1 3.1 0.0401 AF151039 Ubiquitinconjugating enzyme E2 J1 (EC 6.3.2.19) (Noncanonical ubiquitin conjugating enzyme 1) H300014573 3.0 0.0434 U80919 Nuclear factor of activated Tcells, cytoplasmic 1 (NFAT transcription complex cytosolic component) H300005089 SPERT 3.0 0.014 AK093129 testisspecific leucine zipper protein nurit; novel leucine zipper testicular protein; likely ortholog of mouse Nurit H300017056 MUCDHL 2.9 0.037 AK000226 H300009124 GNA15 2.9 0.0426 M63904 Guanine nucleotidebinding protein, alpha15 subunit (Galpha15) (G alpha16). H300003918 ADH4 2.9 0.0161 M15943 Alcohol dehydrogenase class II pi chain precursor (EC 1.1.1.1). H300015508 ALDH3A2 2.8 0.0193 BC002430 Fatty aldehyde dehydrogenase (EC 1.2.1.3) (Aldehyde dehydrogenase, microsomal) (ALDH class 3). H200017546 CP110 2.8 0.0159 BC036654
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300021936 2.8 0.0469 AK090926 H200018958 INADL 2.7 0.0424 AJ001306 InaDlike protein isoform 3; protein associated to tight junctions; H200011183 EIF2AK3 2.6 0.0432 AF193339 Eukaryotic translation initiation factor 2alpha kinase 3
H300016787 2.6 0.0213 AK057898 H200017970 DCT 2.6 0.0249 D17547 Dopachrome tautomerase precursor (EC 5.3.3.12) (DT) (DCT) H300018673 METTL4 2.6 0.0433 AK026670 methyltransferase like 4 H300013544 EDG2 2.6 0.0373 BC036034 Lysophosphatidic acid receptor Edg2 (LPA receptor 1) (LPA1). H300019530 COL8A1 2.6 0.0221 AF170702 Collagen alpha 1(VIII) chain precursor (Endothelial collagen). H300013773 SPECC1 2.5 0.0391 BC033618 H300004322 2.5 0.0183 H200004084 SFRP2 2.5 0.0279 AF311912 secreted frizzledrelated protein 2 precursor; secreted apoptosis related protein 1 H300000558 TMEM132C 2.4 0.0044 AK126715 H200015384 APOBEC2 2.4 0.0189 BC047767 Probable C>U editing enzyme APOBEC2 (EC 3.5.4.). H200005453 MORC4 2.4 0.00854 AK056235 Zinc finger CWtype coiledcoil domain protein 2. H200017196 ADAMTSL1 2.4 0.0231 AF251058 ADAMTSlike protein 1 precursor (ADAMTSL1) (Punctin) (UNQ528/PRO1071). H300005263 2.4 0.00238 BC015353 tripartite motifcontaining 43 H300009986 C17orf49 2.4 0.00626 H300012199 EPB41L4A 2.4 0.0234 AL117425 H200004328 ATAD4 2.4 0.00605 BC002865 H200020273 SOS2 2.4 0.0349 L13858 Son of sevenless protein homolog 2 (SOS2). H200014109 TSCOT 2.4 0.0322 BC048285 thymic stromal cotransporter H300015021 2.3 0.0147 BC034708 Ninein (hNinein). H200021065 2.3 0.0475 BC016831 H200008274 PRKRIR 2.3 0.0168 AF081567 52 kDa repressor of the inhibitor of the protein kinase (p58IPK interacting protein)
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300010689 2.2 0.0168 H300013394 2.2 0.011 H300004507 2.2 0.00456 BC033871 Tripartite motif protein TRIM50B. H300005257 2.2 0.000272 H300000305 GAL3ST2 2.2 0.0205 AB040610 H300001256 NR2C1 2.2 0.0173 M29960 Orphan nuclear receptor TR2. H200002431 SUPT16H 2.2 0.000427 AF152961 chromatinspecific transcription elongation factor large subunit H300004564 F8 2.2 0.00388 H300010544 HLA-DQB2 2.1 0.018 H300005695 2.1 0.00838 AK098031 H300022698 CFLAR 2.1 0.0461 BC001602 CASP8 and FADDlike apoptosis regulator precursor (Cellular FLICElike inhibitory protein) (cFLIP) H200001873 CARD8 2.1 0.0308 AB023172 H300019575 EPB41L4A 2.1 0.00973 AK096057 Band 4.1like protein 4A (NBL4 protein). H200005386 CCDC77 2.1 0.037 AK027638 H300018872 VNN2 2.1 0.00386 D89974 Vascular noninflammatory molecule 2 precursor (Vanin 2) H300010814 SH3GLB1 2.1 0.0242 AB007960 SH3 domain GRB2like protein B1 (EC 2.3.1.) (Endophilin B1) (Bax interacting factor 1) (Bif1) (CGI61). H300016280 PRIM2A 2.1 0.000314 H200003028 TDRD9 2.1 0.0037 AL833915 tudor domain containing 9; hypoxiainducible gene; chromosome 14 open reading frame 75 H200017579 TMEM68 2.1 0.0489 BC043417 H200000768 CASP6 2.1 0.0257 U20536 Caspase6 precursor (EC 3.4.22.) (Apoptotic protease Mch2). H300011388 GAGE8 2.1 0.0196 U19142 GAGE1 protein (G antigen 1) (MZ2F antigen). H200005489 ATAD2B 2.1 0.00131 AB033066 H300006668 PPAPDC2 2.1 0.00333 BC038108 H300004833 SPOCK1 2.1 0.0476 BC030691 Testican1 precursor (SPOCK protein). H200004567 CEP70 2.1 0.0098 BC030598 centrosomal protein 70 kDa
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300002796 DEFB119 2.1 0.0451 AY122473 Betadefensin 120 precursor (Betadefensin 20) (DEFB20). H200014718 KRT5 2.1 0.0413 M21389 Keratin, type II cytoskeletal 5 (Cytokeratin 5) (K5) (CK 5) (58 kDa cytokeratin). H300016647 WDFY4 2.0 0.0313 AK024502 H300016050 RBPMS 2.0 0.000836 D84111 H200002511 SLC22A5 2.0 0.0201 AF057164 Organic cation/carnitine transporter 2 (Solute carrier family 22, member 5) H300001673 UNC5CL 2.0 0.0223 BC035284 unc5 homolog Clike; ZU5 and death domaincontaining protein H300022107 2.0 0.0264 AF151801 H300017419 DCN 2.0 0.0338 NM_133505 Decorin precursor (Bone proteoglycan II) (PGS2) (PG40). H300012073 CHMP4A 2.0 0.0478 AK092821 H300013932 PKNOX1 2.0 0.0365 AY196965 Homeobox protein PKNOX1 (PBX/knotted homeobox 1) (Homeobox protein PREP1). H300003874 C11orf72 2.0 0.000632 AK075315 H300014922 IQWD1 2.0 0.00297 AF116725 IQ motif and WD repeats 1; PC326 protein H200012053 SLC22A1 2.0 0.00643 X98332 solute carrier family 22 member 1 isoform a; organic cation transporter 1 H200004022 GAB2 2.0 0.031 AB011143 GRB2associated binding protein 2 (GRB2associated binder2) (pp100). H300006705 SLC5A9 2.0 0.000301 BC048332 DJ1024N4.1 (Novel Sodium:solute symporter family member similar to SLC5A1 (SGLT1)) (Fragment). H300008917 OR52B2 2.0 0.0437 AB065939 H200012925 ANKRD57 2.0 0.0247 BC066772 H300011362 TBC1D16 2.0 0.00502 AL157485 H300001276 2.0 0.0288 AK095200 H200010283 C13orf3 2.0 0.0376 BC013418 H200003170 C12orf11 2.0 0.00172 AK001499 H300019446 2.0 0.0173 J03756 Somatotropin precursor (Growth hormone) (GH) (GHN) (Pituitary growth hormone) (Growth hormone 1).
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H200006108 HSPA2 2.0 0.00216 BC036107 Heat shockrelated 70 kDa protein 2 (Heat shock 70 kDa protein 2). H300011567 FHL2 1.9 0.0213 U60117 Skeletal muscle LIMprotein 3 (SLIM 3) (LIMdomain protein DRAL) (Four and a half LIM domains protein 2) (FHL2). H300014701 1.9 0.0432 BC036506 NACHT, LRR and PYDcontaining protein 11 (PYRINcontaining APAF1 like protein 6) H300006719 1.9 0.0324 BX647078 H200001667 ZFYVE26 1.9 0.0212 AB002319 zinc finger, FYVE domain containing 26 H300006429 1.9 0.0208 AF130080 H300016247 LOC124446 1.9 0.0028 BC017488 H300022403 BAALC 1.9 0.0436 AK022077 brain and acute leukemia, cytoplasmic H200004430 PHLPP 1.9 0.0139 AB011178 suprachiasmatic nucleus circadian oscillatory protein; H300008546 OR4L1 1.9 0.00263 AB065558 Olfactory receptor 4L1. H300001378 1.9 0.0223 AF041410 Malignancyassociated protein (Fragment). H200011615 PIP5K2A 1.9 0.042 U14957 Phosphatidylinositol4phosphate 5kinase type II alpha (EC 2.7.1.149) (PIP5KIIalpha) H200006426 ATR 1.9 0.000432 Y09077 ataxia telangiectasia and Rad3 related protein; Seckel syndrome; Rad3 related protein; H200011674 TM6SF2 1.9 0.0146 AB067513 Transmembrane 6 superfamily member 2 (Fragment). H300017930 KIAA1199 1.9 0.00924 AB103330 H300010399 CSMD1 1.9 0.0438 AF333704 CUB and sushi multiple domains protein 1 precursor (UNQ5952/PRO19863). H200005248 MOSPD1 1.9 0.0119 AL137163 motile sperm domain containing 1 H200001670 DLC1 1.9 0.0341 AL117604 RhoGTPaseactivating protein 7 (Rhotype GTPaseactivating protein 7) (Deleted in liver cancer 1 protein) H300000258 1.9 0.00115 AL136588 H200015882 FOXP3 1.9 0.00175 AJ005891 Forkhead box protein P3 (Zinc finger protein JM2) (Scurfin). H300018110 SH3RF2 1.8 0.0443 AK074234 SH3 domain containing ring finger 2 H200013119 ABCA12 1.8 0.0402 AY219711 ATPbinding cassette, subfamily A, member 12 isoform a
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300004977 C12orf38 1.8 0.00382 AK056924 H300015292 ACIN1 1.8 0.0159 H200007306 KIAA0406 1.8 0.00477 BC013121 H300002610 1.8 0.00918 H300005581 1.8 0.0179 BC028035 H300007653 LOC441005 1.8 0.0199 XM_498961 H300007517 OR5AY1 1.8 0.032 AB065624 Olfactory receptor 5AY1. H300005519 OR1E2 1.8 0.0139 U76377 Olfactory receptor 1E2 (Olfactory receptor 1793/17135/17136) (OR17 93) (OR17135) (OR17136). H300018894 C2orf3 1.8 0.0191 BC064559 GCrich sequence DNAbinding factor (GCF) (Transcription factor 9) (TCF9). H200015765 RASA2 1.8 0.00545 AF115573 Ras GTPaseactivating protein 2 (GAP1m). H300007247 1.8 0.00724 BM701578 H200012668 ZNRF2 1.8 0.00753 AK025240 zinc finger/RING finger 2 H300013372 RGS5 1.8 0.00193 BX537427 Regulator of Gprotein signaling 5 (RGS5). H200017718 1.8 0.00000189 AK021549 H200016156 HOXA11 1.8 0.0293 BC040948 Homeobox protein HoxA11 (Hox1I). H300006513 1.8 0.00661 AY154469 NACHT, LRR and PYDcontaining protein 14 (Nucleotidebinding oligomerization domain protein 5). H200012652 TEKT2 1.8 0.0366 BC022005 Tektin 2 (Tektint) (Testicular tektin B1like protein). H300010981 1.8 0.0397 H300006669 1.8 0.0148 AK096599 H200015365 KIF2B 1.8 0.0316 AF333335 kinesin protein H300003287 OR5C1 1.8 0.0195 AB065725 Olfactory receptor 5C1 (Olfactory receptor 9F) (OR9F). H200019551 SLC44A4 1.8 0.0168 BC014659 NG22 protein; choline transporterlike protein 4 H300005785 ATP5H 1.8 0.00032 H300021867 SMEK1 1.7 0.0116 AB095930 H300002916 CAMTA1 1.7 0.0288 AB020640 MSTP023. H200015220 G3BP1 1.7 0.00133 BC006997 RasGTPaseactivating protein binding protein 1 (GAP SH3domain binding protein 1)
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H200003962 RNF135 1.7 0.0462 BC082262 ring finger protein 135 isoform 1 H300021559 STRN3 1.7 0.0495 U17989 Striatin 3 (Cellcycle autoantigen SG2NA) (S/G2 antigen). H200013948 CENPC1 1.7 0.0361 M95724 H300006036 1.7 0.0174 S41522 H300020949 DAGLB 1.7 0.00893 AF450090 KCCR13L H300016395 TCF21 1.7 0.036 BC025697 Transcription factor 21 (Podocyteexpressed 1) (Pod1) (Epicardin) (Capsulin). H300021837 IFRD2 1.7 0.00928 BC051750 Interferonrelated developmental regulator 2 (SKMC15 protein). H200002587 PPP1R12A 1.7 0.0344 AF458589 Protein phosphatase 1 regulatory subunit 12A (Myosin phosphatase targeting subunit 1) H300015090 SLC2A9 1.7 0.0219 AF210317 Solute carrier family 2, facilitated glucose transporter, member 9 (Glucose transporter type 9). H200007303 ITGA10 1.7 0.00902 AF112345 Integrin alpha10 precursor. H300014265 PBX3 1.7 0.00252 BC016977 PreBcell leukemia transcription factor3 (Homeobox protein PBX3). H200019380 1.7 0.0172 AF251047 H200007104 CD74 1.7 0.00183 CR595353 H300020671 1.7 0.0168 AK057602 60S ribosomal protein L12. H200011836 DNAI1 1.7 0.0426 AF091619 Dynein intermediate chain 1, axonemal (Axonemal dynein intermediate chain 1). H300006157 FUT4 1.7 0.0329 AF305083 Alpha(1,3)fucosyltransferase (EC 2.4.1.) (Galactoside 3L fucosyltransferase) H300001564 RFESD 1.7 0.0328 BC035110 LOC317671 H200021283 1.7 0.00201 AF275269 Nanos homolog 1 (NOS1). H200009118 1.7 0.023 AK025278 H200019045 IHPK2 1.7 0.00395 AL117458 inositol hexaphosphate kinase 2; mammalian inositol hexakisphosphate kinase 2 H200007947 UBE1DC1 1.7 0.00541 AY253672 ubiquitinactivating enzyme E1domain containing 1 H300016110 PTPLB -1.7 0.000296 AY191814 H200016887 WNT16 -1.7 0.0111 AF169963 Wnt16 protein precursor.
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300000810 -1.7 0.0267 XM_371145 H200014457 C3orf24 -1.7 0.0414 AF230334 H300008841 LOC554175 -1.7 0.00143 H300010888 -1.7 0.0104 AF316855 H300015516 ANGEL2 -1.7 0.0156 BC016966 H300005307 TMEM16G -1.7 0.0321 AY617079 H300001317 BLM -1.7 0.00352 U39817 Bloom's syndrome protein (EC 3.6.1.) (RecQ proteinlike 3) (DNA helicase, RecQlike, type 2). H200000703 PRM1 -1.7 0.0278 Y00443 Sperm protamine P1 (Cysteinerich protamine). H200004555 MGC11102 -1.7 0.0274 AK094129 H200009824 MFSD11 -1.7 0.0000509 BC002753 H300004213 PCP2 -1.7 0.0315 XM_058956 Purkinje cell protein 2 homolog. H300003036 -1.7 0.0436 AK054749 H300011601 USP7 -1.7 0.00246 Z72499 Ubiquitin carboxylterminal hydrolase 7 (EC 3.1.2.15) H300019870 BCL11A -1.7 0.0236 AJ404611 Bcell lymphoma/leukemia 11A (Bcell CLL/lymphoma 11A) H300020233 KCNH1 -1.7 0.0000573 AJ001366 Potassium voltagegated channel subfamily H member 1 (Voltagegated potassium channel subunit Kv10.1) H300019539 MON2 -1.7 0.0313 AL834320 SF21 protein. H200008136 PLEKHO1 -1.7 0.00809 AK125609 CK2 interacting protein 1; HQ0024c protein H200006240 PNRC1 -1.7 0.0213 U03105 Prolinerich nuclear receptor coactivator 1 (Prolinerich protein 2) (B42 protein). H300019935 WHSC1 -1.7 0.0333 AF071594 WolfHirschhorn syndrome candidate 1 protein isoform 3; H300007889 FLJ34047 -1.7 0.0296 AK091366 H300012495 FBXL13 -1.7 0.0018 AK095387 H300003075 USP54 -1.7 0.0326 H300022432 -1.7 0.0192 AF448838 neuropilin and tolloidlike protein 1 isoform 1 precursor H300001993 CRABP1 -1.8 0.0165 AK096006 H300001395 ZNF645 -1.8 0.0228 AK098601 H200005190 TWISTNB -1.8 0.0000258 AC004940 TWIST neighbor H200010469 TRIM31 -1.8 0.0128 X81006 Tripartite motif protein 31.
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300022414 ZMYM5 -1.8 0.0382 AJ133354 Zinc finger protein 237 (Zinc finger protein 198like 1) (HSPC050). H300007380 ERICH1 -1.8 0.0408 BX647093 H300018475 OGFR -1.8 0.00328 AF172453 Opioid growth factor receptor (OGFr) (Zetatype opioid receptor) (760 protein). H300020729 ZSCAN20 -1.8 0.0228 BC008827 Zinc finger protein 31 (Zinc finger protein KOX29) (Fragment). H200010690 FHOD1 -1.8 0.0265 AB041046 FH1/FH2 domainscontaining protein (Formin homolog overexpressed in spleen) (FHOS) H200005381 SEMA3G -1.8 0.00364 AB029496 semaphorin sem2 H200009312 DBF4B -1.8 0.0271 AK023149 H200016290 SLC22A7 -1.8 0.0476 BC033805 solute carrier family 22 member 7 isoform a; organic anion transporter 2; liverspecific transporter H300018062 ADCK4 -1.8 0.0119 H300020414 CNNM2 -1.8 0.0181 AK000071 cyclin M2 isoform 3; ancient conserved domain protein 2 H300006581 -1.8 0.0453 AK094115 H300014751 C3orf63 -1.8 0.0109 AF180425 retinoblastomaassociated protein 140; CTCL tumor antigen se891 H300022195 RAB43 -1.8 0.0191 BC007409 RAB41 protein H300018492 CEACAM1 -1.8 0.0406 X16354 Carcinoembryonic antigenrelated cell adhesion molecule 1 precursor (Biliary glycoprotein 1) (BGP1) H300021568 CCDC71 -1.8 0.0429 AK023691 H300008219 -1.8 0.000471 H300012402 KIAA1245 -1.8 0.0246 AK075065 N2N protein H300022155 RERE -1.8 0.0127 AB007927 arginineglutamic acid dipeptide (RE) repeats; atrophin 1like; arginine glutamic acid dipeptide RE repeats H300001004 -1.8 0.0211 H300004552 -1.8 0.0114 AB049915 H200016466 CCRN4L -1.8 0.00612 AF183961 Nocturnin (CCR4 protein homolog). H300015331 CALD1 -1.8 0.000537 M83216 Caldesmon (CDM). H200005304 -1.8 0.00109 AF017790 kinetochore associated 2; highly expressed in cancer, rich in leucine heptad repeats;
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300021777 TTN -1.8 0.0469 X90568 titin isoform novex3; connectin; CMH9, included; cardiomyopathy, dilated 1G (autosomal dominant) H300003505 FLJ38377 -1.8 0.0254 AK095696 H200016293 -1.8 0.00572 AL049348 H200006395 EGFR -1.8 0.00453 X00588 H200002093 MID2 -1.8 0.0385 Y18880 Midline 2 protein (Midline defect 2) (Tripartite motif protein 1) (Midin 2). H200000058 -1.8 0.00276 L20316 Glucagon receptor precursor (GLR). H300012504 LASP1 -1.9 0.00383 X82456 LIM and SH3 domain protein 1 (LASP1) (MLN 50). H300006988 LOC643905 -1.9 0.00325 AF453950 H300016870 FBLIM1 -1.9 0.00442 AL133035 Filaminbinding LIM protein1 (FBLP1) (Mitogeninducible 2 interacting protein) (MIG2interacting protein) (Migfilin). H300021094 -1.9 0.000735 AK094322 Creatine kinase, ubiquitous mitochondrial precursor (EC 2.7.3.2) H200005376 C1orf176 -1.9 0.0338 AK023245 H300001616 -1.9 0.0215 AK092009 H300012775 COQ7 -1.9 0.00674 H300002177 OR52M1 -1.9 0.0305 AB065789 olfactory receptor, family 52, subfamily M, member 1; olfactory receptor, family 52, subfamily M, H300022434 C11orf17 -1.9 0.0124 AK075308 Prolinerich protein BCA3 (Breast cancer associated gene 3 protein). H300021580 SUZ12 -1.9 0.0184 D63881 Polycomb protein SUZ12 (Suppressor of zeste 12 protein homolog) H300005636 -1.9 0.00283 AK055854 H300016773 CEPT1 -1.9 0.000105 AL832104 choline/ethanolaminephosphotransferase H300013348 FANCD2 -1.9 0.00676 AF230336 Fanconi anemia, complementation group D2 H300000039 PSMD4P2 -1.9 0.0472 BC068549 H200017558 ANXA9 -1.9 0.00271 AJ009985 Annexin A9 (Annexin 31) (Annexin XXXI). H300012292 GABRA1 -1.9 0.0376 X14766 Gammaaminobutyricacid receptor alpha1 subunit precursor (GABA(A) receptor). H300019934 GORASP1 -1.9 0.0318 AK124755 Golgi reassembly stacking protein 1 (Golgi
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H200002776 SLC43A1 -1.9 0.0038 AF045584 solute carrier family 43, member 1; prostate cancer overexpressed gene 1; Ltype amino acid transporter 3 H200001597 TMEM116 -1.9 0.0123 AL833501 H200002988 PVRL3 -1.9 0.0273 BC001336 H300016944 SSFA2 -1.9 0.0202 AB067514 Spermspecific antigen 2 (Cleavage signal1 protein) (CS1). H300001599 -1.9 0.00876 AF035044 H300011599 KRTCAP3 -1.9 0.002 AY358993 keratinocyte associated protein 3; keratinocytes associated protein 3 H300019969 RASGRF2 -1.9 0.0197 AF023130 Ras proteinspecific guanine nucleotidereleasing factor 2 H300010179 LRRN2 -2.0 0.0271 H200004569 PALM2 -2.0 0.00102 AJ303079 Akinase anchor protein 2 (Protein kinase A anchoring protein 2) (PRKA2) (AKAP2). H300014819 ZBTB32 -2.0 0.00899 AF130255 testis zinc finger protein H300018309 CA6 -2.0 0.0351 BC034350 H300006818 -2.0 0.0165 X63097 Blood group Rh(D) polypeptide (Rhesus D antigen) (RHXIII) (Rh polypeptide 2) (RhPII). H300007573 -2.0 0.0188 H300010746 -2.0 0.0000739 H300003237 -2.0 0.00334 AK097866 H300005065 -2.0 0.0142 H300019433 VILL -2.0 0.0474 BC004300 Villinlike protein. H200005576 SFXN5 -2.0 0.00433 AK025170 Sideroflexin 5. H200013471 C14orf50 -2.0 0.00457 AK058044 H300021550 C8orf61 -2.0 0.0146 AF123761 CLN8 protein. H300010425 IL22RA2 -2.0 0.000885 AY040567 interleukin 22binding protein isoform 1; class II cytokine receptor; interleukin 22binding protein H200002519 C6orf211 -2.0 0.0131 AK022972 H200013760 KIAA1524 -2.0 0.0448 AB040957 H200012518 C7orf13 -2.0 0.0237 AF439977 H300004490 C6orf152 -2.0 0.0238 BC050327
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H200002777 CCDC17 -2.0 0.00168 AK057646 H300002409 HS3ST5 -2.0 0.0171 AK126930 heparan sulfate (glucosamine) 3Osulfotransferase 5; heparan sulfate 3OST5 H200011117 UCP3 -2.0 0.00378 BC008392 Mitochondrial uncoupling protein 3 (UCP 3). H300005697 -2.0 0.00106 H200012739 C8orf37 -2.1 0.000674 BC036855 H300004937 PPP1R10 -2.1 0.0228 Y13247 H200008704 APOA5 -2.1 0.0338 AF202889 apolipoprotein AV; regenerationassociated protein 3; apolipoprotein A5 H200004525 RAD50 -2.1 0.0224 U63139 RAD50 homolog isoform 1; RAD50 (S. cerevisiae) homolog H300021922 -2.1 0.019 AB007899 H300011825 FBXW10 -2.1 0.00568 CF593769 Fbox and WD40 domain protein 10; chromosome 17 open reading frame 1A H200016946 FLJ20184 -2.1 0.0096 AK000191 H200017577 TNKS2 -2.1 0.0356 AF438201 Tankyrase 2 (EC 2.4.2.30) (TANK2) (Tankyrase II) (TNKS2) H300011598 -2.1 0.04 BI822669 Fc gamma receptor I H200013087 GPC1 -2.1 0.00934 AF070536 H300018252 RIN2 -2.1 0.000741 AL136924 Ras and Rab interactor 2 (Ras interaction/interference protein 2) H300000023 C8orf42 -2.2 0.00385 AK126209 H200008827 LOC441416 -2.2 0.0489 AK000451 H300019329 LGR6 -2.2 0.00533 AB049405 Leucinerich repeatcontaining G proteincoupled receptor 6. H200018748 -2.2 0.0205 AL512728 H300015451 PNPLA8 -2.2 0.000736 AF263613 intracellular membraneassociated calciumindependent phospholipase A2 gamma H300016665 -2.2 0.014 AK091671 H300016445 -2.2 0.035 AK090618 H300007535 SIRPG -2.2 0.00636 AK093519 H300008996 KIAA1509 -2.2 0.0374 XM_496003
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H200002025 ELF5 -2.3 0.0121 AF115402 E74like factor 5 ESE2b; epitheliumspecific Ets transcription factor 2 H300012693 PFTK1 -2.3 0.0225 AC006036 Serine/threonineprotein kinase PFTAIRE1 (EC 2.7.1.37). H300016174 ZNF451 -2.3 0.044 NM_015555 Zinc finger protein 451 (Coactivator for steroid receptors). H200013629 C9orf131 -2.3 0.0267 AL133575 H300008147 -2.3 0.0278 H300006062 RANBP1 -2.3 0.0435 D38076 H300007089 -2.3 0.00494 AL078621 BA395L14.5 (Novel phosphoglucomutase like protein) (Fragment). H200012127 MAP6 -2.3 0.00258 AB058781 H200008116 DYRK2 -2.4 0.0319 Y13493 Dualspecificity tyrosinephosphorylation regulated kinase 2 (EC 2.7.1.112) (EC 2.7.1.37). H300014409 SAPS3 -2.4 0.0000206 AB046778 sporulationinduced transcript 4associated protein H300007576 TTTY21 -2.4 0.00751 AF527833 H300016124 -2.4 0.00616 AK096057 H300005693 -2.4 0.00525 AF130103 H300021617 -2.4 0.0238 AF503919 methylCpG binding domain protein 3like 2 H300005864 -2.4 0.000673 AK000879 H300010167 -2.5 0.0486 AL832222 H300020846 PDLIM5 -2.5 0.00183 AF061258 LIM protein (similar to rat protein kinase Cbinding enigma); enigma homolog H200012201 ACTL7B -2.5 0.0234 BC033789 H300012276 TMEM29 -2.6 0.000455 BX649013 transmembrane protein 29; PRO0659 protein H300004055 -2.6 0.00894 U52830 H300020883 ADAMTS9 -2.6 0.0271 AB037733 ADAMTS9 precursor (EC 3.4.24.) (A disintegrin and metalloproteinase with thrombospondin motifs 9) H300010507 ADD1 -2.8 0.0424 S70314 Alpha adducin (Erythrocyte adducin alpha subunit). H200004449 SKIL -2.8 0.0444 Z19588 Skilike protein SKIL (Skirelated oncogene). H300003464 -2.9 0.000897 BC031108 H300006664 -2.9 0.0167 XM_497658
Operon ID Gene Symbol AR knockdown vs. control cells Genbank Gene description
Fold Change P-value H300017889 NVL -2.9 0.00192 U68140 Nuclear valosincontaining proteinlike (Nuclear VCPlike protein) (NVLp). H300003294 INSC -3.0 0.000102 XM_370613 H200016296 DIO1 -3.0 0.0283 BC017955 Type I iodothyronine deiodinase (EC 1.97.1.10) (TypeI 5'deiodinase) (DIOI) (Type 1 DI) (5DI). H300022685 COL21A1 -3.0 0.0188 AL136624 alpha 1 type XXI collagen precursor; collagen XXI H300018718 TRPS1 -3.2 0.0135 AF183810 Zinc finger transcription factor Trps1 (Zinc finger protein GC79) (Trichorhinophalangeal syndrome type I protein). H300009481 GNRHR2 -3.2 0.0104 AY077708 Gonadotropinreleasing hormone II receptor (Type II GnRH receptor) (GnRHIIR). H300014850 -3.2 0.016 H200016052 GRIN3B -3.3 0.045 AC004528 Glutamate H200018059 RIMS4 -3.4 0.0442 AK126807 Regulating synaptic membrane exocytosis protein 4 (Rab3 interacting molecule 4) (RIM 4) (RIM4 gamma). H300013638 SYT6 -3.5 0.0161 AK056448 synaptotagmin VI H200012600 NUT -3.6 0.000707 AL137416 nuclear protein in testis H300006417 SH3D19 -3.7 0.0052 BX647422 H200002761 SIPA1L2 -4.0 0.000771 AB037810 Signalinduced proliferationassociated 1 like protein 2 (Fragment). H300015641 tcag7.1314 -4.2 0.0443 AL834358 H300013556 AR -5.5 0.00439 M23263 Androgen receptor (Dihydrotestosterone receptor).