Circulating and Tissue-Resident CD4+ T Cells with Reactivity to Intestinal Microbiota Are Abundant in Healthy Individuals and Function Is Altered During Inflammation
Accepted Manuscript Circulating and Tissue-resident CD4+ T Cells With Reactivity to Intestinal Microbiota Are Abundant in Healthy Individuals and Function is Altered During Inflammation Ahmed N. Hegazy, Nathaniel R. West, Michael J.T. Stubbington, Emily Wendt, Kim I.M. Suijker, Angeliki Datsi, Sebastien This, Camille Danne, Suzanne Campion, Sylvia H. Duncan, Benjamin M.J. Owens, Holm H. Uhlig, Andrew McMichael, Andreas Bergthaler, Sarah A. Teichmann, Satish Keshav, Fiona Powrie PII: S0016-5085(17)35979-6 DOI: 10.1053/j.gastro.2017.07.047 Reference: YGAST 61339 To appear in: Gastroenterology Accepted Date: 25 July 2017 Please cite this article as: Hegazy AN, West NR, Stubbington MJT, Wendt E, Suijker KIM, Datsi A, This S, Danne C, Campion S, Duncan SH, Owens BMJ, Uhlig HH, McMichael A, Oxford IBD Cohort Investigators, Bergthaler A, Teichmann SA, Keshav S, Powrie F, Circulating and Tissue-resident CD4+ T Cells With Reactivity to Intestinal Microbiota Are Abundant in Healthy Individuals and Function is Altered During Inflammation, Gastroenterology (2017), doi: 10.1053/j.gastro.2017.07.047. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. ACCEPTED MANUSCRIPT CFSE Memory CD4+ T cells labelling (CD3+ CD4+ MACS/FACS CFSE A CD45RA- ) dilution Expansion 3-7 days Co-culture C MACS Incubation with 100 **** Monocytes bacterial lysates CD14+ **** 80 S.
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