Aerosol Microbiome Over the Mediterranean Sea Diversity and Abundance
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Microbial Diversity of Molasses Containing Tobacco (Maassel) Unveils Contamination with Many Human Pathogens
European Review for Medical and Pharmacological Sciences 2021; 25: 4919-4929 Microbial diversity of molasses containing tobacco (Maassel) unveils contamination with many human pathogens M.A.A. ALQUMBER Department of Laboratory Medicine, Faculty of Applied Medical Sciences, Albaha University, Saudi Arabia Abstract. – OBJECTIVE: Tobacco smoking drugs in today’s modern world. Different meth- remains a worldwide health issue, and the use of ods are currently used to consume tobacco, in- flavored varieties (maassel) embedded in glyc- cluding cigarettes, cigars and waterpipes1. Water- erine, molasses, and fruit essence via shisha pipe (shisha) smoking continues to rise globally2. paraphernalia (waterpipe) is growing globally. Smoking flavored tobacco (maassel), through the 16S rRNA gene pyrosequencing was conduct- shisha, is becoming a global preventable cause of ed on 18 different varieties representing 16 fla- 3,4 vors and three brands in order to study the mi- morbidity and mortality . crobiota of maassel and find whether it contains Scientists studied the chemical composition of pathogenic bacteria. tobacco for many years and illustrated the total MATERIALS AND METHODS: The samples number of chemicals identified in tobacco during were selected randomly from the most utilized the years from 1954 to 20055. In addition, a com- brands within Albaha, Saudi Arabia as deter- prehensive review of these chemicals’ classifica- mined through a questionnaire of 253 smok- ers. In addition, ten-fold serially diluted sam- tion, concentration and changes with time due ples were inoculated on blood agar, MacConkey to changes in the shape, design and composition agar, half-strength trypticase soy agar and malt of cigarettes was reported almost a decade ago6. -
Taxonomy JN869023
Species that differentiate periods of high vs. low species richness in unattached communities Species Taxonomy JN869023 Bacteria; Actinobacteria; Actinobacteria; Actinomycetales; ACK-M1 JN674641 Bacteria; Bacteroidetes; [Saprospirae]; [Saprospirales]; Chitinophagaceae; Sediminibacterium JN869030 Bacteria; Actinobacteria; Actinobacteria; Actinomycetales; ACK-M1 U51104 Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Limnohabitans JN868812 Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae JN391888 Bacteria; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Planctomyces HM856408 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales GQ347385 Bacteria; Verrucomicrobia; [Methylacidiphilae]; Methylacidiphilales; LD19 GU305856 Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Pelagibacteraceae GQ340302 Bacteria; Actinobacteria; Actinobacteria; Actinomycetales JN869125 Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae New.ReferenceOTU470 Bacteria; Cyanobacteria; ML635J-21 JN679119 Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae HM141858 Bacteria; Acidobacteria; Holophagae; Holophagales; Holophagaceae; Geothrix FQ659340 Bacteria; Verrucomicrobia; [Pedosphaerae]; [Pedosphaerales]; auto67_4W AY133074 Bacteria; Elusimicrobia; Elusimicrobia; Elusimicrobiales FJ800541 Bacteria; Verrucomicrobia; [Pedosphaerae]; [Pedosphaerales]; R4-41B JQ346769 Bacteria; Acidobacteria; [Chloracidobacteria]; RB41; Ellin6075 -
Table S5. the Information of the Bacteria Annotated in the Soil Community at Species Level
Table S5. The information of the bacteria annotated in the soil community at species level No. Phylum Class Order Family Genus Species The number of contigs Abundance(%) 1 Firmicutes Bacilli Bacillales Bacillaceae Bacillus Bacillus cereus 1749 5.145782459 2 Bacteroidetes Cytophagia Cytophagales Hymenobacteraceae Hymenobacter Hymenobacter sedentarius 1538 4.52499338 3 Gemmatimonadetes Gemmatimonadetes Gemmatimonadales Gemmatimonadaceae Gemmatirosa Gemmatirosa kalamazoonesis 1020 3.000970902 4 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas indica 797 2.344876284 5 Firmicutes Bacilli Lactobacillales Streptococcaceae Lactococcus Lactococcus piscium 542 1.594633558 6 Actinobacteria Thermoleophilia Solirubrobacterales Conexibacteraceae Conexibacter Conexibacter woesei 471 1.385742446 7 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas taxi 430 1.265115184 8 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas wittichii 388 1.141545794 9 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas sp. FARSPH 298 0.876754244 10 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sorangium cellulosum 260 0.764953367 11 Proteobacteria Deltaproteobacteria Myxococcales Polyangiaceae Sorangium Sphingomonas sp. Cra20 260 0.764953367 12 Proteobacteria Alphaproteobacteria Sphingomonadales Sphingomonadaceae Sphingomonas Sphingomonas panacis 252 0.741416341 -
Metabarcoding for Bacterial Diversity Assessment: Looking Inside Didymosphenia Geminata Mats in Patagonian Aquatic Ecosystems
Aquatic Invasions (2021) Volume 16, Issue 1: 43–61 Special Issue: Proceedings of the 21st International Conference on Aquatic Invasive Species Guest editors: Sarah Bailey, Bonnie Holmes and Oscar Casas-Monroy CORRECTED PROOF Research Article Metabarcoding for bacterial diversity assessment: looking inside Didymosphenia geminata mats in Patagonian aquatic ecosystems Ana Victoria Suescún1,*, Karla Martinez-Cruz2, Maialen Barret3 and Leyla Cárdenas1,4 1Instituto de Ciencias Ambientales y Evolutivas, Facultad de Ciencias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile 2Environmental Biogeochemistry in Extreme Ecosystems Laboratory, Universidad de Magallanes, Punta Arenas, Chile 3Laboratory of Functional Ecology and Environment, Université de Toulouse, CNRS, Toulouse, France 4Centro FONDAP de Investigación en Dinámica de Ecosistemas Marinos de Altas Latitudes (IDEAL), Chile Author e-mails: [email protected] (AVS), [email protected] (KM), [email protected] (MB), [email protected] (LC) *Corresponding author Co-Editors’ Note: This study was contributed in relation to the 21st International Conference Abstract on Aquatic Invasive Species held in Montreal, Canada, October 27–31, 2019 (http://www.icais. The number of organisms that spread and invade new habitats has increased in recent org/html/previous21.html). This conference has decades as a result of drastic environmental changes such as climate change and provided a venue for the exchange of anthropogenic activities. Microbial species invasions occur worldwide in terrestrial information on various aspects of aquatic and aquatic systems and represent an emerging challenge to our understanding of invasive species since its inception in 1990. The conference continues to provide an the interplay between biodiversity and ecosystem functioning. Due to the difficulty opportunity for dialog between academia, of detecting and evaluating non-indigenous microorganisms, little is known about industry and environmental regulators. -
Alicyclobacillus Spp.: New Insights on Ecology and Preserving Food Quality Through New Approaches
Microorganisms 2015, 3, 625-640; doi:10.3390/microorganisms3040625 OPEN ACCESS microorganisms ISSN 2076-2607 www.mdpi.com/journal/microorganisms Review Alicyclobacillus spp.: New Insights on Ecology and Preserving Food Quality through New Approaches Emanuela Ciuffreda, Antonio Bevilacqua, Milena Sinigaglia and Maria Rosaria Corbo * Department of the Science of Agriculture, Food and Environment, University of Foggia, Via Napoli 15, 71122 Foggia, Italy; E-Mails: [email protected] (E.C.); [email protected] (A.B.); [email protected] (M.S.) * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +39-08-8158-9232. Academic Editor: Giuseppe Comi Received: 27 July 2015 / Accepted: 29 September 2015 / Published: 10 October 2015 Abstract: Alicyclobacillus spp. includes spore-forming and thermo-acidophilic microorganisms, usually recovered from soil, acidic drinks, orchards and equipment from juice producers. The description of the genus is generally based on the presence of ω-fatty acids in the membrane, although some newly described species do not possess them. The genus includes different species and sub-species, but A. acidoterrestris is generally regarded as the most important spoiler for acidic drinks and juices. The main goal of this review is a focus on the ecology of the genus, mainly on the species A. acidoterrestris, with a special emphasis on the different phenotypic properties and genetic traits, along with the correlation among them and with the primary source of isolation. Finally, the last section of the review reports on some alternative approaches to heat treatments (natural compounds and other chemical treatments) to control and/or reduce the contamination of food by Alicyclobacillus. -
Marine Cyanolichens from Different Littoral Zones Are
bioRxiv preprint doi: https://doi.org/10.1101/209320; this version posted February 6, 2018. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Marine cyanolichens from different littoral 2 zones are associated with distinct bacterial 3 communities 4 Nyree J. West*1, Delphine Parrot2†, Claire Fayet1, Martin Grube3, Sophie Tomasi2 5 and Marcelino T. Suzuki4 6 1 Sorbonne Universités, UPMC Univ Paris 06, CNRS, Observatoire Océanologique de Banyuls (OOB), 7 F-66650, Banyuls sur mer, France 8 2 UMR CNRS 6226, Institut des Sciences chimiques de Rennes, Equipe CORINT « Chimie Organique 9 et Interfaces », UFR Sciences Pharmaceutiques et Biologiques, Univ. Rennes 1, Université Bretagne 10 Loire, F-35043, Rennes, France 11 3 Institute of Plant Sciences, University of Graz, A-8010 Graz, Austria 12 4 Sorbonne Universités, UPMC Univ. Paris 06, CNRS, Laboratoire de Biodiversité et Biotechnologies 13 Microbiennes (LBBM), Observatoire Océanologique, F-66650, Banyuls sur mer, France 14 †Current address: GEOMAR Helmholtz Centre for Ocean Research Kiel, Research Unit Marine 15 Natural Products Chemistry, GEOMAR Centre for Marine Biotechnology, 24106 Kiel, Germany 16 *Corresponding author: 17 Observatoire Océanologique de Banyuls sur mer, F-66650 Banyuls sur mer, France 18 19 Tel: +33 (0)4 30 19 24 29, Fax: +33 (0)4 68 88 73 98 20 Email: [email protected] 21 1 bioRxiv preprint doi: https://doi.org/10.1101/209320; this version posted February 6, 2018. -
Dynamics of Naphthenic Acids and Microbial Community Structures in A
Electronic Supplementary Material (ESI) for RSC Advances. This journal is © The Royal Society of Chemistry 2017 Supporting Information: Dynamics of naphthenic acids and microbial community structures in a membrane bioreactor treating oil sands process- affected water: impacts of supplemented inorganic nitrogen and hydraulic retention time 1, 2 1, 3 1 1 Jinkai Xue , Yanyan Zhang , Yang Liu , Mohamed Gamal El-Din 1 Department of Civil and Environmental Engineering, University of Alberta, Edmonton, Alberta, T6G 1H9, Canada; 2 Department of Civil, Environmental, and Geo- Engineering, University of Minnesota, Minneapolis, Minnesota 55404, United States; 3 Department of Civil Engineering, New Mexico State University, Las Cruces, New Mexico, United States Supporting information includes 7 tables, 5 figures, and 17 pages in total. 1 Water chemistry analyses Samples were collected weekly from the feed by using a disposable syringe, and from the permeate by leaving the effluent tubing in a clean glass bottle untill a volume of 100 mL was achieved. Right after sample collection, Thermo Scientific™ Target2™ Nylon Syringe Filters (0.22 μm) were used to filter water samples prior to analyses. Conventional water chemistry parameters including pH, COD, nitrate nitrogen (NO3-N), ammonium nitrogen (NH4-N), and nitrite nitrogen (NO2-N) were measured for the feed and permeate samples in duplicates according to the standard methods (Federation and Association, 2005). To measure the mixed liquor suspended solids (MLSS) and mixed liquor volatile suspended solids (MLVSS) concentration (an indicator of microbial growth), a 30 mL sludge sample was collected weekly from the middle depth of the anoxic and aerobic tanks by using a disposable syringe. -
Novel Method of Probe Design for Characterising Unclassified Microbial Taxa in Wastewater
Novel method of probe design for characterising unclassified microbial taxa in wastewater TAN SHI MING Interdisciplinary Graduate School The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) 2017 Novel method of probe design for characterising unclassified microbial taxa in wastewater TAN SHI MING Interdisciplinary Graduate School The Singapore Centre for Environmental Life Sciences Engineering (SCELSE) A thesis submitted to the Nanyang Technological University in partial fulfilment of the requirement for the degree of Doctor of Philosophy 2017 Acknowledgements First and foremost, I will like to express my gratitude towards my supervisor, Professor Yehuda Cohen for providing me with the academic freedom to pursue my PhD candidature in SCELSE. I thank him for the countless opportunities he has provided, the financial stability for the projects that I have pursued and his strong resolve in moulding me into an independent researcher. Secondly, I will like to express my thanks to Dr Paul William, Mr Teo Guo Hui and Mr Ryan Lim for their tremendous help with the flow cytometry work. The flow cytometry work would not have gone smoothly without their invaluable expertise and advice. Prof Federico Lauro had proposed the use of FISH-FACS tool for the enrichment of the unclassified bacteria and I appreciate his input. The sequencing team in SCELSE has helped with the sequencing aspect of my thesis. Special thanks go to Dr Daniela Moses whom I have consulted on the type of sequencing platform to use and Mr Alexander Putra who has efficiently handled my samples for sequencing. Mr Larry Liew was instrumental in obtaining sludge samples from Ulu Pandan Water Reclamation Plant, and I will like to thank him for his time and effort. -
Halophilic Bacteroidetes As an Example on How Their Genomes Interact with the Environment
DOCTORAL THESIS 2020 PHYLOGENOMICS OF BACTEROIDETES; HALOPHILIC BACTEROIDETES AS AN EXAMPLE ON HOW THEIR GENOMES INTERACT WITH THE ENVIRONMENT Raúl Muñoz Jiménez DOCTORAL THESIS 2020 Doctoral Programme of Environmental and Biomedical Microbiology PHYLOGENOMICS OF BACTEROIDETES; HALOPHILIC BACTEROIDETES AS AN EXAMPLE ON HOW THEIR GENOMES INTERACT WITH THE ENVIRONMENT Raúl Muñoz Jiménez Thesis Supervisor: Ramon Rosselló Móra Thesis Supervisor: Rudolf Amann Thesis tutor: Elena I. García-Valdés Pukkits Doctor by the Universitat de les Illes Balears Publications resulted from this thesis Munoz, R., Rosselló-Móra, R., & Amann, R. (2016). Revised phylogeny of Bacteroidetes and proposal of sixteen new taxa and two new combinations including Rhodothermaeota phyl. nov. Systematic and Applied Microbiology, 39(5), 281–296 Munoz, R., Rosselló-Móra, R., & Amann, R. (2016). Corrigendum to “Revised phylogeny of Bacteroidetes and proposal of sixteen new taxa and two new combinations including Rhodothermaeota phyl. nov.” [Syst. Appl. Microbiol. 39 (5) (2016) 281–296]. Systematic and Applied Microbiology, 39, 491–492. Munoz, R., Amann, R., & Rosselló-Móra, R. (2019). Ancestry and adaptive radiation of Bacteroidetes as assessed by comparative genomics. Systematic and Applied Microbiology, 43(2), 126065. Dr. Ramon Rosselló Móra, of the Institut Mediterrani d’Estudis Avançats, Esporles and Dr. Rudolf Amann, of the Max-Planck-Institute für Marine Mikrobiologie, Bremen WE DECLARE: That the thesis titled Phylogenomics of Bacteroidetes; halophilic Bacteroidetes as an example on how their genomes interact with the environment, presented by Raúl Muñoz Jiménez to obtain a doctoral degree, has been completed under our supervision and meets the requirements to opt for an International Doctorate. For all intents and purposes, we hereby sign this document. -
Bacteria Associated with Vascular Wilt of Poplar
Bacteria associated with vascular wilt of poplar Hanna Kwasna ( [email protected] ) Poznan University of Life Sciences: Uniwersytet Przyrodniczy w Poznaniu https://orcid.org/0000-0001- 6135-4126 Wojciech Szewczyk Poznan University of Life Sciences: Uniwersytet Przyrodniczy w Poznaniu Marlena Baranowska Poznan University of Life Sciences: Uniwersytet Przyrodniczy w Poznaniu Jolanta Behnke-Borowczyk Poznan University of Life Sciences: Uniwersytet Przyrodniczy w Poznaniu Research Article Keywords: Bacteria, Pathogens, Plantation, Poplar hybrids, Vascular wilt Posted Date: May 27th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-250846/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/30 Abstract In 2017, the 560-ha area of hybrid poplar plantation in northern Poland showed symptoms of tree decline. Leaves appeared smaller, turned yellow-brown, and were shed prematurely. Twigs and smaller branches died. Bark was sunken and discolored, often loosened and split. Trunks decayed from the base. Phloem and xylem showed brown necrosis. Ten per cent of trees died in 1–2 months. None of these symptoms was typical for known poplar diseases. Bacteria in soil and the necrotic base of poplar trunk were analysed with Illumina sequencing. Soil and wood were colonized by at least 615 and 249 taxa. The majority of bacteria were common to soil and wood. The most common taxa in soil were: Acidobacteria (14.757%), Actinobacteria (14.583%), Proteobacteria (36.872) with Betaproteobacteria (6.516%), Burkholderiales (6.102%), Comamonadaceae (2.786%), and Verrucomicrobia (5.307%).The most common taxa in wood were: Bacteroidetes (22.722%) including Chryseobacterium (5.074%), Flavobacteriales (10.873%), Sphingobacteriales (9.396%) with Pedobacter cryoconitis (7.306%), Proteobacteria (73.785%) with Enterobacteriales (33.247%) including Serratia (15.303%) and Sodalis (6.524%), Pseudomonadales (9.829%) including Pseudomonas (9.017%), Rhizobiales (6.826%), Sphingomonadales (5.646%), and Xanthomonadales (11.194%). -
Soil Microbial Communities in Bioenergy Cropping Systems: Unearthing Relationships Across a Heterogeneous Agroecosystem Sarah Kate Hargreaves Iowa State University
Iowa State University Capstones, Theses and Graduate Theses and Dissertations Dissertations 2014 Soil microbial communities in bioenergy cropping systems: unearthing relationships across a heterogeneous agroecosystem Sarah Kate Hargreaves Iowa State University Follow this and additional works at: https://lib.dr.iastate.edu/etd Part of the Ecology and Evolutionary Biology Commons, Microbiology Commons, and the Soil Science Commons Recommended Citation Hargreaves, Sarah Kate, "Soil microbial communities in bioenergy cropping systems: unearthing relationships across a heterogeneous agroecosystem" (2014). Graduate Theses and Dissertations. 14003. https://lib.dr.iastate.edu/etd/14003 This Dissertation is brought to you for free and open access by the Iowa State University Capstones, Theses and Dissertations at Iowa State University Digital Repository. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Iowa State University Digital Repository. For more information, please contact [email protected]. Soil microbial communities in bioenergy cropping systems: unearthing relationships across a heterogeneous agroecosystem by Sarah Kate Hargreaves A dissertation submitted to the graduate faculty in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY Major: Ecology and Evolutionary Biology Program of Study Committee: Kirsten S. Hofmockel, Major Professor Michael J. Castellano Larry J. Halverson Thomas M. Isenhart Timothy P. Parkin Iowa State University Ames, Iowa 2014 -
Type of the Paper (Article
Supplementary Materials S1 Clinical details recorded, Sampling, DNA Extraction of Microbial DNA, 16S rRNA gene sequencing, Bioinformatic pipeline, Quantitative Polymerase Chain Reaction Clinical details recorded In addition to the microbial specimen, the following clinical features were also recorded for each patient: age, gender, infection type (primary or secondary, meaning initial or revision treatment), pain, tenderness to percussion, sinus tract and size of the periapical radiolucency, to determine the correlation between these features and microbial findings (Table 1). Prevalence of all clinical signs and symptoms (except periapical lesion size) were recorded on a binary scale [0 = absent, 1 = present], while the size of the radiolucency was measured in millimetres by two endodontic specialists on two- dimensional periapical radiographs (Planmeca Romexis, Coventry, UK). Sampling After anaesthesia, the tooth to be treated was isolated with a rubber dam (UnoDent, Essex, UK), and field decontamination was carried out before and after access opening, according to an established protocol, and shown to eliminate contaminating DNA (Data not shown). An access cavity was cut with a sterile bur under sterile saline irrigation (0.9% NaCl, Mölnlycke Health Care, Göteborg, Sweden), with contamination control samples taken. Root canal patency was assessed with a sterile K-file (Dentsply-Sirona, Ballaigues, Switzerland). For non-culture-based analysis, clinical samples were collected by inserting two paper points size 15 (Dentsply Sirona, USA) into the root canal. Each paper point was retained in the canal for 1 min with careful agitation, then was transferred to −80ºC storage immediately before further analysis. Cases of secondary endodontic treatment were sampled using the same protocol, with the exception that specimens were collected after removal of the coronal gutta-percha with Gates Glidden drills (Dentsply-Sirona, Switzerland).