Supplementary Table 1) Immunohistochemical Protocol and Antibody Information Antibody Company Clone/# Clonality Dilution Incubat
H. Reis et al: Differential proteomic and tissue expression analyses identify valuable diagnostic biomarkers of hepatocellular differentiation and hepatoid adenocarcinomas Suppl ementary Table 1) Immunohistochemical protocol and antibody information Antibody Company Clone/# Clonality Dilution Incubation Antigen retrieval Detection ABAT Abcam EPR4433 mono 1/3000 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP ACAA2 Abcam EPR6733 mono 1/100 30 min, RT pH 9.0, WB, 95°C, 20 min Zytomed Polymer HRP ACADM Abcam EPR3708 mono 1/3000 30 min, RT pH 9.0, WB, 95°C, 20 min Zytomed Polymer HRP ADH1B Abcam 4F12 mono 1/12.000 30 min, RT pH 9.0, WB, 95°C, 20 min Zytomed Polymer HRP Arginase1 Abcam EPR6672(B) mono 1/1000 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP BHMT Abcam EPR6782 mono 1/100 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP FABP1 Acris AIV\09011PU-S mono 1/15.000 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP HAOX1 Acris AP18044PU-N poly 1/100 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP HepPar1 Dako OCH1E5 mono 1/800 30 min, RT pH 9.0, WB, 95°C, 20 min. Zytomed Polymer HRP HMGCS2 Abcam Ab104807 poly 1/50 60 min, RT pH 9.0, WB, 95°C, 20 min Zytomed Polymer HRP H. Reis et al: Differential proteomic and tissue expression analyses identify valuable diagnostic biomarkers of hepatocellular differentiation and hepatoid adenocarcinomas Supplementary Table 2) Composition of the non-liver tumor TMA Diagnosis n % ADC 11 2.9 ADC Adenocarcinoma of the lung Carc.
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