okf"kZd izfrosnu ANNUAL REPORT
2012-2013
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jk"Vªh; i'kq vkuqoaf'kd lalk/ku C;wjks (Hkk-Ï-v-i-) djuky & 132001 (gfj;k.kk) Hkkjr National Bureau of Animal Genetic Resources (ICAR) Karnal - 132001 (Haryana) India NBAGR
ANNUAL REPORT 2012-2013 NBAGR E-mail: [email protected] Tel: 0184-4043541,3292951 Karnal -132001,Haryana 343, 1stFloor,MughalCanal Market, Intech Printers&Publishers Printing Ms. InduBala,StenoGr.III Dr. PSDangi(TechnicalOfficer,T-7/8) Assistance Sh. MotiRam,T-5 Photographs Sh. Satpal,T-5 Dr. SaketKumarNiranjan,SeniorScientist Dr. RekhaSharma,SeniorScientist Dr. AvnishKumar,SeniorScientist Dr. AKMishra,PrincipalScientist Dr. RSKataria,PrincipalScientist Dr. PKSingh,PrincipalScientist Dr. NKVerma,PrincipalScientist Editors & I/C,PMECell Dr. NKVerma,PrincipalScientist Compiled by Karnal -132001(Haryana)India National BureauofAnimalGeneticResources Dr. BKJoshi,Director Published by Line... Credit 2012-
Network ProjectonAnGR Functional Genomics Genetic Characterizationand Conservation 12 Phenotypic Characterizationand Livestock InformationManagement Research Accomplishments Financial Outlay Organogram 4 About Bureau History andProfile Executive Summary Foreword i-ii jktHkk"kk izdks"Bdhxfrfo/k;k¡ Transfers/Joinings Appointments/Promotions Skilled SupportingStaff Administrative Technical Scientific Personnel Teaching 64 Distinguished Visitors Fairs/Exhibitions 63 Sports Activities Celebrations 62 and organized Trainings /Workshopsattended Institute TechnologyManagementUnit Important Meetings Library Services Other Activities Awards/Recognition Publications Research Projects Research Projects&Publications 13 Contents
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jk-i-vk-la-C;wjks NBAGR FOREWORD I feel immensely pleased to present the Annual Report 2012-13 of National Bureau of Animal Genetic Resources. The trajectory of the following pages is aimed at shedding light on glimpses of our rich livestock biodiversity so far characterized, evaluated, conserved and documented by the Bureau during last one year. Twenty nine years ago, on 21st September, 1984 the Bureau got officially off the ground and is now marching ahead with renewed vigour to overcome the complex challenges and threats particularly in checking the loss of livestock biodiversity and enhancing their sustainable utilization. Within this short period, the Bureau has become an internationally renowned center of excellence for research on characterization, conservation and sustainable development of AnGR. Nature has bestowed upon our country with vast and varied forms of resources and ecological conditions which have enriched our country with immense biodiversity including domestic animal diversity. Farm livestock species are one of the major constituent of this biodiversity which comprised of large spectra of farm animal breeds. National Bureau of Animal Genetic Resources under its mandate of Identification, Evaluation, Characterization, Conservation and Utilization of livestock and poultry genetic resources has characterized and documented most of the well known breeds of different farm animal species. So far 37 breeds of cattle, 13 of buffalo, 39 of sheep, 23 of goat, 2 of pig, 6 of Horse & Ponies, 8 of camel, 1 of donkey and 15 of poultry have been registered. These characterized and classified descript breeds constitute about 25- 30% of total population of our farm animal resources whereas rest forms the non-descript animals which so far are inadequately studied. Now the efforts are to characterize, evaluate and document lesser known and unexplored animal populations and ultimately transform the nondescripts into well descript and registered breeds.
During the year scientists of the Bureau concentrated their efforts on conducting breed surveys, characterizing and establishing the physical attributes of Sanchori cattle of Rajasthan, local cattle of Mizoram, native cattle of Andhra Pradesh, Koraput sheep of Odisha, Raigari, Malkangiri and Narayanpatna goats of Odisha, Harringhata Black chicken of West Bengal and Sindhi donkey of Rajasthan. Conservation is another important activity which the Bureau is looking into by undertaking ex-situ and in-situ conservation programmes on the genetically eroded breeds and critically declining population. Under ex-situ conservation programme, the Gene Bank has added collection of around 15 thousands semen doses and now its inventory has more than 1 lakh cryo-preserved semen doses produced from 276 breeding males of 38 breeds of seven farm animal species. In the field also superior germplasm of purebred cattle, sheep and goat have been disseminated for the genetic enhancement of local livestock of the native ecologies. Now our focus is on conducting groundbreaking genomic research for proper understanding the unique attributes of the native AnGR. De-novo assembly of goat and camel transcriptome has been generated. The researchers are engaged in emerging areas of science for analyzing
molecular basis of important economical and environmentally adaptive traits viz. milk production, milk ANNUAL REPORT 2012-2013 constituents, meat yield & quality, disease resistance, thermoregulation, prolificacy, draftability, down to the smallest details under various institutional and externally funded projects. 2012-13 i NBAGR
ANNUAL REPORT 2012-2013 ii work and cooperat thanks are due to my dynamic team of scientists, administrative, and technical supporting staff fortheir hard from DrKMLPathak, Deputy Director and (Animal Sciences) DrSCGupta, General (AP&B).Special ADG for ICAR, General, his guidance, help and support. acknowledge Ialso cooperation the and encouragement I sincerely thank DrSAyyappan, Secretary, Department of Agricultural Research and Education &Director excelled inmany and events. sports other social fellow received colleagues, who awards the for year the 2012.Apart from research, the staff the of Bureau has technical, administrative and supporting staff withhave been recognized also awards. Icongratulate all my year. conferredbeing every Similarly promoting competitive spirit for excellence inworking, workers best in excelling field in the of characterization and conservation of native livestock institutedbreedsbeen has and is presented at various fora. To encourage researchers, the Dr. P. G.Nair Award for Institute’s the scientist monographs were published also during year. the Scientists have rewarded been for their research achievements and international journals of impact viewed high factors. peer Sixteen popular/technical articles and seven were commercialized. on research the Based findingsscientific 62 papers been have published the in national Three technologies i.e. DNA markerkits based forparentage verification in zebu cattle, camels and buffaloes initiated for developing technologies,patentsthe filing theirand commercialization has shown results.good patents,filing protocols, models anddatabase packages for storage, and analysisretrieval data.of Efforts Various research projects culminated with results have laid foundation the for development of technologies, new NBAGR 2012- ion. 13 (B. K.Joshi) Director jk-i-vk-la-C;wjks NBAGR
jk-i-vk-la-C;wjks NBAGR EXECUTIVE SUMMARY National Bureau of Animal Genetic Resources During the period under report scientists of and National Institute of Animal Genetics were Bureau strived hard to achieve the objectives set up on 21st September, 1984 and started at by working under various institute and Regional Station of National Dairy Research externally funded research projects. The salient Institute Bangalore. Bureau and institute were achievements emerged from different projects then shifted to Karnal in 1985 and temporarily are summarized below: housed in NDRI Campus before shifting to its own campus at Makrampur, Karnal in 1994. The The database on Livestock Genes and Proteins Institute of Animal Genetics and Bureau were was enriched with gene-ontology data collected merged in 1995 to function as a single unit as from ftp site (http://www.geneontology.org/ EXECUTIVE SUMMARY National Bureau of Animal Genetic Resources. GO.downloads.html). Presently 35,113 gene- National Bureau of Animal Genetic Resources, ontology records have been stored in the Karnal has been the nodal organization in India database. User-interface using ASP.NET was with the mandate - ‘Identification, evaluation, created to search proteins through gene-ontology characterization, conservation and utilization terms. Coding for classifying livestock proteins of livestock and poultry genetic resources of the on the basis of cellular component, biological country’ and is working to achieve the following function and molecular function was also objectives: completed.
YY To conduct systematic surveys to Nine new breeds of livestock species were characterize, evaluate and catalogue farm registered and assigned accession numbers. livestock and poultry genetic resources and This included three breeds of cattle (Pulikulam, to establish their national database. Kosali, Malnad Gidda), one buffalo (Kalahandi), two each of goat (Konkan Kanyal, Berari) and YY To design methodologies for ex-situ pig (Ghoongroo, Niang Megha) and one of conservation and in-situ management and donkey (Spiti). optimal utilization of farm animal genetic resources. Exploratory visits were conducted in the breeding tracts of different livestock breeds/ YY To undertake studies on genetic populations and data were collected on characterization using modern techniques phenotypic and biometric traits. Information of molecular biology. on management practices, socioeconomic parameters were collected through interactions Y Y To conduct training programmes as related with farmers. Following this systematic approach, ANNUAL REPORT 2012-2013 to evaluation, characterization and utilization characterization of Sanchori cattle of Rajasthan, of animal genetic resources. indigenous cattle of Mizoram, local cattle of 2012-13 iii NBAGR
ANNUAL REPORT 2012-2013 iv EXECUTIVE SUMMARY Under conducting systematic in native the surveys tract. Sindhi donkey of Rajasthan was characterized by distribution area. under extensive management system from their morphometric traits of 224adult sheep reared were characterized by data collecting on seven Lonand, Sangamneri Madgyal, and Solapuri Five of ecotypes sheep i.e. Deccani Kolhapuri, Harringhata Black chicken of West Bengal. Information to was characterize collected state. buffaloGojri of Himachal Pradesh and Punjab breeding and tract management practices of conducted to document migratory routes/ of Odisha were accomplished. was also Survey and Raigari, Narayanpatna and Malkangiri goats Adilabad (A.P), Koraput district sheep of Odisha NBAGR was assessed throughwas assessed analysing cattle mtDNA Cattle admixture in domestic populations yak heterozygosity except for Gir. heterozygosity was less than expected the 84 microsatellite was analyzed. loci Observed patterns across three the populations using for Sahiwal, Gir and Hariana cattle, allelic the As an attempt to develop breed the signature Sheep, Camel, Equine and Yak). breeds of (Cattle, species seven Buffalo,Goat, belongingfrozen eight doses semen to thirty at Bank Gene NBAGR now stores about 1,09,223 to .Theadded repository Bank inGene National and breed 1buffalo procured has been and of doses semen 10cattle breeds 14,150 frozen ex-situ 2012- conservation programme Karan Fries cows was analysed during peri- (PMN) cells inMurrah Sahiwal buffaloes, and adaptation of circulating polymorpho nuclear Comparative physiological and transcriptional indicated aheterozygosity excess at loci. these values at SNP the were all loci negative which inTNFβpromoterobserved region and Fis inIFN-γgene.were Total observed 6SNPs were polymorphic microsatellite repeats (GTTT)n in 5Zebu cattle breeds. Total 20SNPs and (TNF β) of TNFgene the were characterized IFN-γgenecytokines, and lymphotoxin part For studying genetic variability inbovine amplified. were UTRs to corresponding oBLG activator gland. inamammary Fragments of expressionthe of human tissue plasminogen lactoglobulin (oBLG) gene was takento drive Promoter and regulatory elements of ovine beta- for mammalian culture cell expression system. tissue plasminogen activator gene construct An attempt was made for development of human 5.12%. Polyunsaturated fatty acids (PUFA) contributed fatty acids (MUFA) contributed 24.13%and acid was monounsaturated 28.89inwhich methyl ester (FAME). The unsaturated fatty 0.002 to 100gram of 41.70gram per Fatty acid The value of different fatty acids fromvaried was accomplished through gas chromatography. Fatty acidprofiling of 500 samplesgoat milk total 15haplotypes. characterized in Arunachali which revealed yak, total of 37mitochondrial DNA sequences were sequences indomestic populations. yak A 13 jk-i-vk-la-C;wjks NBAGR
jk-i-vk-la-C;wjks NBAGR parturient period. Transcriptome analysis of in expression profile of buffalo and Sahiwal cow buffalo PMN cells identified several hundred mammary epithelial cells during early lactation. differentially expressed genes throughout the A total 65 genes were found to be upregulated peripartum period. The microarray based while only 5 genes were down regulated in analysis revealed several pathways that were buffalo during early lactation period. significantly affected. Results revealed Sahiwal to be more resistant to physiological stress during De-novo assembly of goat transcriptome was the peripartum period. developed using Illumina 2X75 bp paired end reads. The mean contig length was 1815.79 In order to unravel genetic structure of Indian with GC contents of 50.75%. The gene ontology cattle, seven native cattle breeds-Amritmahal, analysis revealed 21718, 22805 and 21901 entries
Gir, Ongole, Red Kandhari, Sahiwal, Tharparkar, from biological processes, cellular component EXECUTIVE SUMMARY Leh cattle and two exotic breeds-Holstein and and molecular functions. Jersey cattle were genotyped using 770K high density bovine SNP chip. The native cattle from Transcriptome data of 76 bp, paired end reads Leh and laddakh region were genetically distinct on 38 various tissues was generated for the from rest of the Indian cattle. comparison in the five biological groups, represented by five breeds (Kanniadu, Black Differential heat shock response was assessed in Bengal, Sirohi, Osmanabadi and Changthangi) mammary epithelial cells of Holstein Friesian, from different geographical locations. The Sahiwal cows and Murrah buffaloes. The higher expression profile revealed 1348 expressed abundance of HSP mRNA after heat stress protein coding genes to be compared among all showed initial evidence of transcriptional the breeds. differences in cattle as well as buffaloes, further suggesting their differential cellular tolerance to De-novo assembly of dromedarian camel withstand heat stress. Heat shock proteins were transcriptome was genrated using Illumina 2X75 found to be upregulated. bp paired end reads. The mean contig length was 1311.03 with GC contents of 51.24%. The Transcriptome profile of buffalo mammary transcriptome assembly had 32940 hits in NCBI gland across different physiological stages was nr-database. The gene ontology analysis revealed compared in heifer and involution stages from 24676, 26027 and 24949 entries from biological lactating stage to identify stage specific genes. processes, cellular components and molecular Several genes related to apoptosis, immune and functions. A total of 581614 accessions were oxidative stress were found to be up-regulated obtained for dromedarian camel. during involution. For identification of QTL for milk yield, ANNUAL REPORT 2012-2013 The transcriptome data was subjected for fat and protein percentage in buffaloes, a comprehensive analysis to reveal key differences total of 30 microsatellite loci from the cattle 2012-13 v NBAGR
ANNUAL REPORT 2012-2013 vi EXECUTIVE SUMMARY differentiation factor 8(GDF8), β3adrenergic polymorphismGenetic at growth the within Indianobserved cattle. was least polymorphic. Sufficient diversity was highest number of SNPs and Kangyam the Hariana and Gir cattle were found to have the showed minimum number of SNPs. Kankrej, cattle breeds.TLR6 was most diverse TLR9 while 9 and10) region coding were found inIndian Total 156SNPs inTLRs(TLR1, 2,4,5,6,8, expressionhigher inacute mastitis incattle. SAA3, Hp, PGLYRP1 and genes CCL2 had frequencies mastitis between and healthy cattle. genes had significantly (p<0.05)differentallelic region. SNPs at PGLYRP1 and haptoglobin showed internal duplication of large coding characterized inbuffalo. Buffalo haptoglobin associatedGenes with incidences of mastitis were compared with non-affected animals. pro-inflammatorycytokines and inmilk blood Murrah animals showed higher expression of showed haplotypes. seven Mastitis affected Murrah, Toda and Swamp which buffaloes, of TLR2and TLR4genes were genotyped in distribution. allelic varied Ecto-domain regions buffalo,cattle and goat. Buffalo revealed breeds 5’-upstream TLR8gene was characterized in associated with QTL the for weights. body foranalysed identification the of markers chromosome. Heifers with different age were with agenetic distance of 10-15cMon each genotyped usingbeen microsatellite markers daughters. Total 8buffalo chromosomes have genome database were amplified in8400 buffalo NBAGR 2012- Kachchhi and Kharai camel from Gujarat, Molecular characterization was done for overlapped with Marwari and Sindhi. Kathiawari didnot form distinct cluster but horses forming two separate clusters, whereas population. Results showed Marwari and Sindhi Kathiawari, and Marwari Sindhi horses Microsatellite were loci studied inthree indigenous breeds were identified. and Sindhi. Total 19SNPs to genes specific equine breeds of India Kathiawari, viz., Marwari CD147 genes were for screened SNPs inthree Myostatin, Monocarboxylate transporters and gene of Indian goats. goats. Three novel SNPs were identified in KiSS1 mutation inBMPRIB exonic regions inIndian absence of any polymorphism including FecB the BMPR1B gene. analysis Sequence revealed were screened for mutations in GDF9 and Indian goat breeds having different prolificacy chromosomal abnormalities. Some of bulls were the to identified have were for screened parameters. cytogenetic their Under project total 423breeding service the bulls variability. presence of reasonably levels of high genetic using microsatellite markers. Results indicated breed genetic diversity and genetic bottlenecks Spiti donkey population was evaluated for within breeds. documented in differentbeen Indian sheep having effect on meat parametersquality has receptor (ADRB3) and calpain (CAPN) genes 13 jk-i-vk-la-C;wjks NBAGR
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Results indicated that Kharai breed is genetically National Advisory Board on Management of different from Kachchhi breed. Genetic Resources meeting was held at NBAGR on 5th March, 2013. The meeting was chaired by Three indigenous pig populations viz., Votho pig Dr. R.S. Paroda, Ex Director-General, ICAR and from Nagaland, indigenous pig from Arunachal co-chaired by Dr. S. Ayyappan, Secretary DARE Pradesh and Mali pig from Tripura of Northeast & DG, ICAR. India were characterized using 21 polymorphic microsatellite loci. Three patents as parentage testing kits for goat and Indian ruminant livestock and DNA test A total of six SNPs were identified in IGF-1 for differentiation of cattle & buffalo meat and gene among three draft cattle breeds. Promoter milk were filed to the Indian Patent Office, Delhi. microsatellite locus at the gene suggested to be a EXECUTIVE SUMMARY potential marker for draughtability in Kangayam, Three technologies on parentage verification Bargur and Hallikar breeds of cattle. in Zebu cattle, camels and buffaloes were commercialized, generating a revenue of total For conservation of Krishna Valley Cattle, total Rs. 6.00 Lakhs. 10,228 inseminations were performed since inception. So far 725 males and 627 females Scientific findings were published by the scientists were born in the breeding tract. For conservation through 62 research papers in national and of Kilakarsal sheep, a total of 488 Kilakarsal international research journals of high impact progenies were produced. factor. Apart from this seven monographs/ leaflets were also brought out. In the Bureau library to keep track of the current scientific/technical developments 48 One scientist was deputed for overseas journals (Fourteen foreign and thirty four Indian participations in seminars/trainings. Journals) were subscribed. In the current year, books and journals worth Rs. 8, 30, 846/- were NBAGR staff participated in different sports procured for the benefit of NBAGR staff. events held in North Zone and bagged medals in volleyball smashing & shooting and race The Institute Research Committee meetings competitions. were held in time. The progress of research projects was reviewed during mid term Institute 29th Foundation Day of the Bureau, National Research Committee (IRC), Research Advisory Science Day, Republic Day and Independence Committee (RAC) meetings. The final reports Day were celebrated at NBAGR campus. of four completed projects were deliberated and Biodiversity Day was celebrated with Gujjar five new project proposals were approved during migratory pastoralists at Shazadpur, Ambala the annual IRC meeting. At present, 27 research (Haryana). Breed Saviour Award function was ANNUAL REPORT 2012-2013 projects including 1 DBT, 6 NAIP and 1 National held on 8th March 2013 at NBAGR. Twenty Fellow project are ongoing in addition to the farmers belonging to different states were newly approved projects. 2012-13 vii NBAGR
ANNUAL REPORT 2012-2013 viii EXECUTIVE SUMMARY provided by Bureau through imparting trainings, members. The consultancies/services were work by is undertaken being various faculty Teaching and guiding of students for thesis their offaculty NDRI, Karnal and IVRI,Izatnagar, NBAGR scientists have included been inthe and exhibitions installed on its various activities. Bureau participated invarious fairs animal/dairy was held on 11.1.2013. One day Workshop on “Awareness of IPR issues” conservation of indigenous germplasm. honoured for contributions their in breed NBAGR 2012- workers were promoted intime. superannuation and transfers. The eligible staff The staffstrength fluctuated new by joining, supportingand 5skilled personnel. staff scientific, 18 technical, 19 administrative, The Bureau work force is comprising of 32 Bureau and interacted with scientists. foreign dignitaries visited laboratories the of Many distinguished personalities including the training programmes. as resourceacted persons for conducting the evaluationcytogenetic of breeding bulls and 13 jk-i-vk-la-C;wjks NBAGR NBAGR
History and Profile
• About Bureau • Organogram • Financial Outlay ANNUAL REPORT 2012-2013
2012-13 1 ANNUAL REPORT 2012-2013 2 2012- 13 NBAGRHISTORY AND PROFILE
jk-i-vk-la-C;wjks NBAGR Abo ut b ureau
About Bureau he need for the establishment of National Institute of Animal Genetics was accepted in principle Tduring 4th Five Year Plan. During 5th and 6th Five Year Plan, various government agencies coordinated the efforts for the establishment of the Institute. Therefore, National Bureau of Animal Genetic Resources and National Institute of Animal Genetics were set up on 21st September, 1984. These institutes started at Regional Station of National Dairy Research Institute, Bangalore. Bureau and the institute were then shifted to Karnal in 1985 and temporarily housed in NDRI Campus before shifting to its own campus at Makrampur, Karnal in 1994. Both Institute and the Bureau were merged to function as a single unit as National Bureau of Animal Genetic Resources in 1995.
National Bureau of Animal Genetic Resources (NBAGR), Karnal has been the nodal organization in India with the mandate and objectives as given below:
MANDATE ‘Identification, evaluation, characterization, conservation and utilization of livestock and poultry genetic resources of the country.
OBJECTIVES
• To conduct systematic surveys to characterize, evaluate and catalogue farm livestock and poultry genetic resources and to establish their National Data Base. • To design methodologies for ex-situ conservation and in-situ management and optimal utilization of farm animal genetic resources.
• To undertake studies on genetic characterization using modern techniques of molecular ANNUAL REPORT 2012-2013 biology. • To conduct training programmes as related to evaluation, characterization and utilization of animal genetic resources. 2012-13 3 NBAGR
ANNUAL REPORT 2012-2013 4 ORGANOGRAM NBAGR HISTORY ANDPROFILE 2012- 13 jk-i-vk-la-C;wjks NBAGRHISTORY AND PROFILE
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Financial Outlay
Financial outlay under Plan and Non-Plan & Network Project of NBAGR for the financial year 2012-13 alongwith expenditure. (Rs. In Lakhs) Sr. HEAD NON-PLAN PLAN Network Project No. Receipt Expenditure Receipt Expenditure Receipt Expenditure 01. Capital FINANCIAL O UTLAY i) Works 5.00 4.70 103.70 103.70 0.00 0.00 ii) Other capital expenditure 5.00 4.72 42.00 38.07 0.00 0.00 Total Capital 10.00 9.42 145.70 141.77 0.00 0.00 02. Revenue 67.00 64.47 i) Establishment expenses 591.97 564.91 0.00 0.00 0.00 0.00 ii) Traveling Allowance 2.89 2.41 12.00 11.89 0.00 0.00 iii) Research & Operational expenses 20.00 19.66 74.90 74.89 0.00 0.00 iv) Administrative Expenses 64.99 64.75 89.60 89.59 0.00 0.00 v) Miscellaneous expenses 4.63 3.46 1.50 1.46 0.00 0.00 Total Revenue 684.48 655.19 178.00 177.83 67.00 64.47 03. Pension & Retirement benefits 20.00 11.47 0.00 0.00 0.00 0.00 Grant Total 714.48 676.08 323.70 319.60 67.00 64.47
Revenue generated during the year 2012-2013 (Rs. in lakhs) Sr. No. Head of Account Amount 1. Sale of Publication & Advertisement 1.30 2. Licence fee 2.70 3. Training Programs - Income 0.40 4. Hostel and Guest house rent 3.40 5. Sale of Technology 5.02 6. Sale of farm Produce 9.46 7. Others Misc. Revenue Receipts 4.07 Total 26.35
Revenue Target Fixed : 30.00 Lakhs ANNUAL REPORT 2012-2013 Target Achieved : 26.35 Lakhs 2012-13 5 NBAGR
ANNUAL REPORT 2012-2013 6 FINANCIAL OUTLAY NBAGR HISTORY ANDPROFILE 2012- 13 jk-i-vk-la-C;wjks NBAGR NBAGR
Research Accomplishments
• Livestock Information Management • Phenotypic Characterization and Conservation • Genetic Characterization and Functional Genomics • Network Project on AnGR ANNUAL REPORT 2012-2013
2012-13 7 2012-13 RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR Livestock Information hircus), duck (Anas platyrhynchos), horse (Equus Management caballus) and sheep (Ovis aries) were inserted into the database. It contains more than 270,000 Establishment of National Agricultural EST records on livestock and poultry species. Bioinformatics Grid (NABG) in ICAR. Bioinformatics tool such as CAP3 for formation of contigs from selected EST’s was incorporated A database on genetic characterization of Animal Genetic Resources (AnGR) of India has been into the database. developed at the institute. User-Login system for The database on Livestock Genes and Proteins the database was developed using ASP.NET with was enriched with gene-ontology data collected C#. Two types of access have been provided to the from ftp site (http://www.geneontology.org/ user- View level and Data-submission level. After GO.downloads.html). Perl scripts were written online registration of a user, its credentials are and executed to store gene-ontology records into verified before granting permission for data entry. the database. Presently 35,113 gene-ontology records have been stored in the database. User- The database on Livestock-EST was enriched with interface using ASP.NET was created to search additional data. User interface of the database was proteins through gene-ontology terms. Coding also enhanced. EST data was downloaded from for searching livestock proteins on the basis of internet resources and Perl scripts were written to gene ontology terms like cellular component, parse the files with different formats and fill the biological function and molecular function was data into the database. EST’s on various tissues also completed. Perl scripts for tools such as O N MANAGEMENT LIVEST O CK INF RMATI for livestock species viz.taurus cattle (Bos taurus), conversion of protein sequence to DNA sequence Pig (Sus scrofa), chicken (Gallus gallus), yak (Bos and counting amino acids for a particular protein were implemented in the database. grunniens), zebu cattle (Bos indicus), goat (Capra ANNUAL REPORT 2012-2013
2012-13 9 NBAGR
ANNUAL REPORT 2012-2013 10 LIVESTOCK INFORMATION MANAGEMENT and drought tolerance. horns with broad and base; are excellent inheat medium having sized; long, strong, circled half and of Rayagada districts Odisha. Animals are for andused milk draught inKalahandi purpose one of buffalo,twoeach of goat and pig and include which species, three breeds of cattle, registration of breeds nine new of livestock on 14 Breed registration Committee inits meeting breeds Registration of livestock and poultry NBAGR Resear Kalaha 01 Donkey 02 01 PIG 02 01 GOAT 03 02 01 CATTLE 01 BUFFALO S.N. th : ndi buffalo May, 2012at New approved Delhi Spiti Niang Megha Ghoongroo Berari Konkan Kanyal Malnad Gidda Kosali Pulikulam Kalahandi Breed 2012- ch Accomplishents
These These buffaloes are type; dual Himachal Pradesh Meghalaya West Bengal Maharashtra Maharashtra Karnataka Chhattisgarh Tamilnadu Odisha Home Tract INDIA_DONKEY__0600_SPITI_05001 INDIA_PIG_1300_NIANGMEGHA_09002 INDIA_PIG_2100_GHOONGROO_09001 INDIA_GOAT_1100_ BERARI _06023 INDIA_GOAT_1100_ KONKANKANYAL _06022 INDIA_CATTLE_0800_MALNADGIDDA_03037 INDIA_CATTLE_2600_KOSALI _03036 INDIA_CATTLE_1800_PULIKULAM_03035 INDIA_BUFFALO_1500_KALAHANDI_01013 Accession number native area. asparasitic compared diseases to crossbreds of Breed is more resistance to communicable and games like bull riding (Jallikattu) area. inlocal manurecattle These purposes. in are used also region of Tamilnadu. Used for draught and in large migratory herds inMadurai and nearby cattle: Pulikulam camel, 2for pig, 1for donkey and 15for chicken. for goat, 39for sheep, 6for horses& ponies, 8for include144, which 37for cattle, 13for buffalo, 23 number of indigenous breeds is country inthe including registered newly these breeds, total indigenoustime pig and donkey breeds. After one of donkey. The institute has registered first 13 These cattle These are maintained jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
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Kosali cattle: Small sized, draft purpose cattle Ghoongroo pig: Reared for pork and manure breed of Chhattisgarh. Farmers prefer bullocks purpose in Darjeeling and nearby Tarai area of of this breed for cleaning of weeds from paddy West Bengal. Animals are black in colour and have upwardly curved snout and large heart field. Animals are known for efficient working shaped ears. ability and high disease resistance.
Malnad Gidda cattle: Short statured cattle Niang Megha pig: Niann Megha pig are breed from Western Ghat of Karnataka, reared distributed in Garo, Khasi and Jaintia hills of for milk and manure purpose. Animal have Meghalaya and is reared for pork and bristle excellent endurance power and resistance to purpose. The animals have typical wild look with diseases. erect bristles on dorsal midline and small erect ears extended vertically. O N MANAGEMENT LIVEST O CK INF RMATI
Konkan Kanyal goat: Meat type goat breed Spiti donkey: This breed is native to Lahaul and adapted to high rainfall and hot and humid Spiti region of Himachal Pradesh. These donkeys climate of Konkan region of Maharasthra. are used for transportation at high altitude Animals have typical white bands on black face (around 3500m above MSL) area with low level and black ear with white margin. of environmental oxygen. These animals can survive well in scarcity of feed and fodder during harsh winter months when the area is completely snow bound.
Berari goat: Reared mainly for meat purpose in Vidarbh region of Maharashtra. As a unique feature, animals have light to dark strips on lateral sides from horn base to nostrils of face. ANNUAL REPORT 2012-2013
2012-13 11 NBAGR
ANNUAL REPORT 2012-2013 12 PHENOTYPIC CHARACTERIZATION AND CONSERVATION NBAGR Resear months, respectively. Average yield is milk peak is reported 3.0-3.5years to be period and 12-15 draft use. The age at first calving and inter-calving gaushalas area, calves male the are either sent to nearby the to adequate mechanization of agriculture inthe black. Tail switch is black, greyish or mixed. Due Hoofmuzzle seen. is also colour is invariably is black inmajority of animals; however, pinkish Hairs are short and straight. The muzzle colour colour. is bigger than Kankrej Body the cattle. cattle. Bulls are white, grey or black incoat in sizeand diameter as compared to Kankrej animals is generally white and horns are smaller to different agegroups Coat coloursex. and of traits were recorded for 312animals belonging Standard eight measurements body and physical the openthe housing system. are hardy seasons in the and inall well survived day time for aduration of 4-6 hours. The animals innearbypracticed fields agricultural the during dhanis farmer’s house fields agricultural intheir called 2 to 20animals open houses inthe adjacent to the cattleDesi are kept herd inthe from sizevarying Sanchori from place the of habitat. their Sanchor/ of Rajasthan state. The animals derivethe name Sanchor and adjoining tehsils of Jalore district Breeding of tract Sanchori cattle includes Sanchori cattle and Conservation Phenotypic Characterization . The animals are fed grazingandstall is 2012- or to sold farmers the of other places for ch Accomplishents Sanchori cow Sanchori like H.S., FMD and BQ islike generally H.S., FMD and practiced. BQ available herds. in the Vaccination against diseases and kachha. Breeding is natural. Breeding bulls are for and milk meat. Animal houses are mostly open power and manure; few farmers rear very them Mizoram cattle are reared mainly for drought Herd sizewas larger in Kolasib district. ranges from 5 to 25 indigenous cattle farmer. per annual income is around 30%.The herd size The contributionfrom livestocksectorthe in farmers have less than annual 1.0lacs, income. from from Champhai. The annual income of farmers ranged holdings are larger inKolasib than the district holding ranges from 0.20to 12ha. Theland of age. Total 88%farmers are literate. Land Majority of farmers the (66%)are 30to 40years state.the Atotal of 33farmers were interviewed. ofin 11 villeges Champhai and Kolasib of districts Indigenous cattle of Mizoram were characterized Indigenous cattle of Mizoram might cause the be of dilution breed. inthe of semen some the Kankrej cases, which is used, Sanchori cattle. The breeding of cows is natural. In farmers are shifting to havethe of insteadbuffaloes marketing milk the on fat the is based percent, some co-operativesto milk the or to gaushalas the . Since, 5.0% fat and 8.5 to 8.8% SNF. sold either is The milk varies from 8 to12 months. contains The milk 4.5 to reported from 6to 18litres. Thelactation length ` Rs. 0.75 lacs to however Rs.0.75lacs 3.2lacs of half the 13 Sanchori bull jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
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36.15±0.37 and 67.26±0.76 cms respectively. The corresponding averages in bullocks (84) are 109.03±1.39, 106.92±0.84, 139.52±1.59 cm, 146.64±1.80, 15.55±0.55, 18.20±0.18 cm, 38.73±0.39 and 68.54±1.07 cm, respectively.
Indigenous cattle of Adilabad (Jhari cattle)
Mizoram cattle Phenotypic characterization of local cattle from Adilabad district was done under NAIP Animals are reared mainly on extensive system of Harmonizing biodiversity sub project. The utility management i.e. grazing from morning to evening. of these cattle is primarily draft and agricultural Animals are wellbuilt, small in size with operations & transport, followed by dairy and cylindrical type of body with strong legs. The dung purpose. Phenotypic measurements body colour varies in different colours viz. brown and breed characteristics on 300 local cattle (85%), black (11%) and gray (44%). Dewlap and were recorded. Average body length, height hump is small. Ears are small to moderate in at withers, chest girth, paunch girth, ear length and horizontal in orientation. Horns are length, tail length and body weight (kg) of small and black (72%) or gray (28%) in colour. cow are 96.24±2.09, 102.52±1.66, 133.83±3.5, Orientation is mostly outward, upward and 136.60± 3.89, 19.40±0.34, 78.03±2.46 cm and then curved towards face. Udder is small and 181.12±13.01kgs, respectively. In adult male the developed. Milk veins are not prominent. Teats body length, height at withers, chest girth, paunch are small (5-12 cm) in size, and funnel (78%) girth, ear length, tail length and body weight (kg) type. Tail switch is brown (54%), black (39%) are 116.02±1.68, 120.33±1.17, 154.70±1.85, and gray (7%). Temperament is docile. 153.01±1.78, 20.76±0.21, 87.93±0.54 cm and
263.98±9.55kgs, respectively. About 60% of O N O N AND C NSERVATI PHEN O TYPIC CHARACTERIZATI The birth weight ranges from 10 to 20 kg. The the animals have white coat colour and rest are cow and bullock weigh about 169 kg and 200 kg, brown coat colours. Animals are medium in size respectively. The daily milk production ranges with well developed dewlap. Horns are small to from 1.0 to 3.5 kg. The average milk yield is medium sized, curved forward, cylindrical and 1.54+0.11 kg. A pair of bullock may plough sickle shaped. The milk production in females about 0.5 acre of land in 5-6 hours. The age at ranges from 1.5 to 2.0 kg per day. Natural service first calving, lactation length, dry period, service is preferred by the farmers for breeding their period and calving interval ranges from 28 to 42 animals. months, 150 to 210 days, 4 to 6 months, 3 to 4 months and 12 to 24 months, respectively. Gojri buffalo
The average body length, height at wither, heart Gojri buffalo is reared by the Gujjar communities girth, paunch girth, horn length, ear length, face from Mohali, Roopnagar, Nawashehar, ANNUAL REPORT 2012-2013 length and tail length without switch in cows Hossiarpur, Sundernagar, Pathankot districts in (71) are 103.70±1.01, 103.60±0.62, 132.22±1.05, Punjab and Nurpur, Jassur, Chawari, Jyot, Sahu, 131.90±1.15, 11.01±0.42, 18.02±0.33, Rakh, Bharmaur and Tissa areas in Kangra and 2012-13 13 NBAGR
ANNUAL REPORT 2012-2013 14 PHENOTYPIC CHARACTERIZATION AND CONSERVATION migration around takes 10to15 days. division of Himachal Pradesh. The one way of (4-5 months) year to reach every Chamba continue last of till September to early November plains /foot during hills mid-May to June and only. Migration by Gujjar pastoralists from starts from areas these are engaged inbuffalo rearing Chamba divisions of Himachal Pradesh. Gujjars NBAGR Resear lactation (38)other than firstlactation. (30) and 5.25,3.45,5.20,9.39,14.64for pooled 5.02, 9.073,14.11,respectively for firstlactation SNF and total inpercentages Solids are 5.04,3.33, constituent milk the traits. Fat, protein, lactose, Khudakalan of Ambalaregion and analyzed for different of villages Raipurrani block and differentlactations from were three collected samplesMilk cattle (n=68)from under Belahi cattle Belahi enclosures.housed inclosed are mostly housed inopen, but young ones are extant buffalo thebreeds in region. Adult animals distinct phenotypicpossess appearance from reared for and milk are found unique to be and called ‘ towards front to complete loop, the locally orientation moves which backwards and then Horns are medium to large with curved sized hairs; Males are brownish to black incolour. The Animals have black coat colourwith brown Average herd sizeranges from 3to 12buffaloes. Pattih wale seengh’. buffaloes Gojri are 2012- ch Accomplishents Gojri buffalo Gojri the othersthe followed by Sangamneri sheep. measures of sheep were Madgyal than higher Thedifferences body ecotypes. the between findingsbased on morphometrictraits indicated migration during to June. December Preliminary Shepherdspractices were observed. remain on colours. of Almost similar types managemental that five all ecotypes have observed differentcoat recorded from distribution their area. It was under extensive management system were length and length) tail of 224adult sheep reared height at withers, paunch ear chest girth, girth, morphometric traits weight, length,(body body Animal Husbandry, Maharashtra. Data on seven area of five the withDepartment ecotypes the of exploratory visit corroborated distribution the study is to characterize and them. An classify Sangamneri and Solapuri. The aim the of present sheep known as Kolhapuri, Lonand, Madgyal, host districts These five ecotypesDeccani of Sangli, Kol Pune, viz. Ahmadnagar,districts Satara, Solapur, sheep isDeccani widely distributed over eight ecotypes sheep Deccani Kolhapuri Madgyal hapur, Nasik of Maharashtra. and Beed 13 Solapuri Sangamneri Lonand jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
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Adult body weight of Madgyal, Sangamneri, Solapuri, Kolhapuri and Lonand ewes were 38.7, 33.4, 30.4, 28.8 and 27.8 kg respectively. In view of preliminary findings, detailed survey of Lonand and Sangamneri sheep were conducted. Data on body biometry of 64 adult sheep and body weight of 24 lambs of Lonand sheep were recorded. Similar data on 145 adult sheep and 45 lambs of Sangamneri sheep were recorded. One hundred blood samples of Sangamneri sheep Koraput ram and fifty samples of Lonand sheep were collected to examine the differences at molecular level. DNA of the blood samples was extracted using standard protocol. It was observed that most of the Lonand sheep rearers have introduced Madgyal rams in their sheep flocks for having better growth of the offspring.
Koraput sheep Koraput sheep is a lesser known sheep breed of Odisha state. Phenotypic data on about 250 adult Koraput ewe animals, body weight on 160 lambs of different Microsatellite markers. The observed number of age groups and information on management alleles in different loci ranged from 3 to 12 with a practices, socio economic utility of this sheep mean value of 6.47±2.92 per locus. The observed from 36 sheep farmers were recorded. Data heterozygosity was 0.57±0.20 and expected analysis based on body biometry revealed that heterozygosity was 0.69±0.17. The average
Koraput sheep is quite distinct from the Ganjam heterozygosity value was 0.6848±0.1671. The O N O N AND C NSERVATI PHEN O TYPIC CHARACTERIZATI sheep found in the same breeding tract. Fis value was found to be 0.1552 which indicate 15.52% inbreeding. The genotyping of 27 Samples of Koraput sheep population was carried out using 21
Table: Body biometry (Mean± SE) of Koraput sheep Trait Koraput Ewes (N=113) Koraput Rams (N=14) Body Length(cm) 51.08±0.34 48.64±0.89 Height wither(cm) 55.58±0.42 56.50±0.99 Chest girth(cm) 66.34±0.45 67.43±0.77 Paunch girth(cm) 69.69±0.56 70.00±1.72 Ear length(cm) 4.61±0.23 4.86±0.52 Horn length (cm) - 8.82±1.18 Tail length(cm) 9.77±0.15 9.29±0.22 ANNUAL REPORT 2012-2013 Face length(cm) 14.44±0.10 15.36±0.23 Body Weight (kg) 18.89±0.35 18.32±0.82 2012-13 15 NBAGR
ANNUAL REPORT 2012-2013 16 PHENOTYPIC CHARACTERIZATION AND CONSERVATION The flock size varied The size from flock varied 5 to35. neck.the Muzzle and hooves are generally black. is black. In black males ring is present around of incase nose brown colour goats. The top line brown colour extending from of base ear the to The face is havingseen. strips whiteof of light however, white and black colour goats are also The goats are mostly light brown or black brown; in sizeand weight and are reared for meat only. of Odisha state. Malkangiri goats are medium goatof local population of Malkangiri district group were recorded for eight parameters body A total o goat Malkangiri NBAGR Resear brown to dark brown and some mixed coloured Goats are medium insize. colour Coat is light oflandless area the tribes for meat only. purpose are reared mainly by marginal farmers small and 40 farmers of were 10villages recorded. Goats from goats known 126lesser /local belonging to Data on and biometry management practices goats of Odisha. length, ear length and length) of tail Raigari paunch facelength, hornchest girth, girth, length, height (body biometry atbody withers, Data were recorded on phenotypic attributes and goat Raigari 6-12 months 3-6 months 0-3 months Group Age f 147animals of different age sex and 2012- Malkangiri buck ch Accomplishents 14.13±0.64 (16) 10.94±0.38 42) 8.01±0.62 (24) (N=82) Male Table: Average weight (kg.)of Koraput body lambs. status of goat the keepers through goat keeping. better economic return foreconomicthe lift goats should multiplied be and improved for 25 animals. Natural is practiced. These service are given to goats. the sizevaries Flock from 2to shelter. No concentrate extra or supplementations up of planks/ are bamboos wooden as goat used Thatched houseswith floor raised (2-4 ft) made 10-12 months and 15-18months, respectively. at maturity sexual and age at firstkidding are and respectively. 22.69±0.61kg, The average age 9.06±0.33, 11.40±0.37,15.0±0.45,16.38±0.40 weight at 3,6,9,12months and adult age are respectively The inmales. overall average body 12.33±0.39, 58.96±1.68and 15.17±1.25cm, 55.46±1.43, 55.92±1.72,13.42±0.51,6.42±0.78, ±0.16 cm.,respectively infemale and 53.75±1.60, ±0.16, 7.87±0.36,12.79±0.18,65.88±0.89,11.95 are 57.79±0.67,58.58±0.58,62.01±0.75,14.76 length, ear length, paunch and length tail girth at withers, facelength, horn chest/heart girth, year. The overall averages length,body height hours grazing during day the throughout the under extensive management system with 6-10 hooves are black or grey. These goats are reared line.nose Muzzle colour is black or brown and ear to muzzle are present on either sideof the brown colour strips extending from of base the flocks. in the White seen animal also or light 13.24±0.67 (28) 9.74±0.40 (28) 7.76±0.48 (22) Female (N=78) 13 Raigari goat jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
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Table: Average body measurements of Raigari goats
Age Sex Height Body Chest Paunch Face Horn Ear length Tail Weight Group (No.) at length Girth Girth Length length (cm) length (kg) Wither (cm) (cm) (cm) (cm) (cm) (cm) (cm)
Female 47.67 44.44 47.44 51.89 11.78 2.33 10.33 10.11 9.00 (9) ±0.99 ±0.58 ±1.23 ±1.24 ±0.46 ±0.41 ±0.37 ±0.31 ±0.37
Male 47.88 46.00 47.63 50.75 10.63 2.50 11.00 10.75 9.13 3 months (8) ±1.09 ±2.12 ±1.27 ±1.16 ±0.32 ±0.38 ±0.38 ±0.37 ±0.58
Overall 47.76 45.18 47.53 51.35 11.24 2.41 10.65 10.41 9.06 ±0.71 ±1.03 ±0.85 ±0.84 ±0.32 ±0.27 ±0.27 ±0.24 ±0.33
Female 50.43 48.43 52.14 54.57 13.86 4.43 10.57 10.29 11.29 (7) ±1.59 ±0.84 ±0.94 ±2.21 ±0.26 ±0.57 ±0.37 ±0.29 ±0.42
Male 55.33 50.00 50.33 55.00 13.00 5.00 11.00 11.33 11.67 6 months (3) ±1.33 ±1.53 ±0.33 ±1.73 ±0.58 ±0.58 ±0.58 ±0.33 ±0.88
Overall 51.90 48.90 51.60 54.70 13.60 4.60 10.70 10.60 11.40 ±1.36 ±0.74 ±0.70 ±1.58 ±0.27 ±0.43 ±0.30 ±0.27 ±0.37
Female 53.86 55.00 56.71 59.43 14.00 5.29 11.71 10.43 14.86 (7) ±1.70 ±1.96 ±1.21 ±1.66 ±0.31 ±0.68 ±0.42 ±0.37 ±0.70
Male 55.25 55.50 58.25 61.00 14.75 7.50 11.75 11.25 15.25 9 months (4) ±1.49 ±1.44 ±2.02 ±2.55 ±0.48 ±0.50 ±0.25 ±0.63 ±0.25
Overall 54.36 55.18 57.27 60.00 14.27 6.09 11.73 10.73 15.00 ±1.18 ±1.31 ±1.03 ±1.35 ±0.27 ±0.56 ±0.27 ±0.33 ±0.45
Female 58.55 57.45 59.82 62.82 14.73 6.91 11.82 11.09 16.09 (11) ±0.88 ±1.19 ±0.70 ±1.04 ±0.19 ±0.62 ±0.23 ±0.28 ±0.39 O N O N AND C NSERVATI PHEN O TYPIC CHARACTERIZATI
Male 58.50 59.00 60.50 65.00 15.50 7.50 12.50 12.00 18.00 12 months (2) ±2.50 ±2.00 ±0.50 ±0.00 ±0.50 ±0.50 ±1.50 ±1.00 ±1.00
Overall 58.54 57.69 59.92 63.15 14.85 7.00 11.92 11.23 16.38 ±0.79 ±1.04 ±0.59 ±0.90 ±0.19 ±0.53 ±0.26 ±0.28 ±0.40
Female 61.35 60.87 65.85 70.06 15.34 9.38 13.62 12.66 22.69 (68) ±0.44 ±0.53 ±0.62 ±0.85 ±0.17 ±0.39 ±0.18 ±0.18 ±0.65
Male 63.43 61.71 65.14 67.14 15.43 10.57 14.71 13.57 22.71 Adult (7) ±1.49 ±1.43 ±2.10 ±2.43 ±0.78 ±1.29 ±0.42 ±0.69 ±1.54
Overall 61.55 60.95 65.79 69.79 15.35 9.49 13.72 12.75 22.69 ±0.42 ±0.50 ±0.59 ±0.81 ±0.16 ±0.37 ±0.17 ±0.17 ±0.61
Figure in parentheses indicate number of observations. ANNUAL REPORT 2012-2013
2012-13 17 NBAGR
ANNUAL REPORT 2012-2013 18 PHENOTYPIC CHARACTERIZATION AND CONSERVATION Figure inparentheses indicate number of observations. NBAGR Resear and 13.80±0.18 cm, respectively. The average 70.69±0.61, 15.62±0.10,9.64±0.33, 13.94±0.24, cm and 61.79±0.37, 63.20±0.39, 67.37±0.51, 15.80±0.22, 11.00±0.76,15.13±0.52,15.27±0.44 62.67±0.99, 65.53±1.02,67.53±0.86,69.47±1.21, length (TL)inadult and male female are horn length (HL),ear length (EL)and tail paunch (PG), face length(CG), (FL), girth (BL), height at withers (BH), chest girth management system. The average lengthbody goatsThese are reared on extensive semi- Age group Age 3 months 3 6 months 6 9 months 9 12 months Adult 2012- ch Accomplishents Sex (No.)Sex (7) Female (3) Male Ovearll (8) Female (4) Male Overall Overall (10) Female (6) Male Overall Female Male Overall Female Male Overall Table: Average measurements body (cm)of goats Malkangiri (cm) Wither at Height ±0.57 47.29 ±1.67 51.33 ±0.85 48.50 ±1.16 50.88 ±1.31 58.25 ±1.35 53.33 ±0.79 54.80 ±1.52 55.50 ±0.73 55.06 ±1.04 58.00 ±0.58 56.00 ±0.84 57.54 ±0.39 63.20 ±1.02 65.53 ±0.37 63.56 (cm) length Body ±1.07 44.00 ±1.33 46.33 ±0.88 44.70 ±0.70 47.75 ±1.49 50.75 ±0.77 48.75 ±0.77 53.00 ±1.05 53.17 ±0.60 53.06 ±0.90 54.60 ±1.15 56.00 ±0.74 54.92 ±0.37 61.79 ±0.99 62.67 ±0.35 61.93 (cm) Girth Chest ±0.83 46.14 ±0.33 48.67 ±0.69 46.90 ±1.11 50.25 ±1.32 56.50 ±1.21 52.33 ±0.50 55.90 ±0.65 56.83 ±0.40 56.25 ±0.76 59.70 ±1.76 57.67 ±0.72 59.23 ±0.51 67.37 ±0.86 67.53 ±0.45 67.40 service is practiced in area. is practiced service maturity at about 12-18months of age. Natural respectively.kg, Female animals show sexual and animal male are 23.93±0.55and 26.73±0.90 respectively. body Theweights of adult female 11.13±0.52, 13.20±0.63,16.30±0.68kg, correspondingthe values were 8.14±0.34, respectively,16.67±0.33 kg, whereas, for female of age were 9.00±0.00,12.25±0.48,14.50±0.85, weightbody of at males 3,6,9 and 12months (cm) Girth Paunch ±1.76 47.57 ±1.53 51.00 ±1.37 48.60 ±1.48 53.50 ±0.87 58.50 ±1.22 55.17 ±1.51 58.70 ±1.10 57.00 ±1.03 58.06 ±1.19 60.90 ±1.00 61.00 ±0.92 60.92 ±0.61 70.69 ±1.21 69.47 ±0.55 70.50 (cm) Length Face ±0.20 11.57 ±0.67 12.67 ±0.28 11.90 ±0.26 12.63 ±0.48 13.25 ±0.24 12.83 ±0.16 13.40 ±0.33 13.67 ±0.16 13.50 ±0.23 14.10 ±0.67 14.33 ±0.22 14.15 ±0.10 15.62 ±0.22 15.80 ±0.09 15.65 13
(cm) length Horn ±0.20 1.43 ±0.33 2.33 ±0.21 1.70 ±0.53 2.63 ±1.31 4.25 ±0.58 3.17 ±0.48 4.40 ±0.70 4.83 ±0.39 4.56 ±0.47 6.00 ±0.67 5.67 ±0.38 5.92 ±0.33 9.64 ±0.76 11.00 ±0.30 9.85
(cm) length Ear ±0.43 11.43 ±0.33 11.67 ±0.31 11.50 ±0.58 12.88 ±0.65 12.50 ±0.40 12.67 ±0.57 12.90 ±0.58 13.00 ±0.40 12.94 ±0.47 13.70 ±0.33 13.67 ±0.36 13.69 ±0.24 13.94 ±0.52 15.13 ±0.22 14.13 jk-i-vk-la-C;wjks
(cm) length Tail ±0.43 10.43 ±0.33 11.33 ±0.33 10.70 ±0.38 11.38 ±0.29 11.50 ±0.26 11.42 ±0.56 11.70 ±0.67 12.67 ±0.43 12.06 ±0.62 12.40 ±0.33 13.33 ±0.49 12.62 ±0.18 13.80 ±0.44 15.27 ±0.18 14.03
RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR Narayanpatna goat 1.05, 9.60 ± 2.38, 15.20 ±0.86, 16.20 ± 0.37 cm, respectively and in female 68.47 ±0.56, 65.63 ± Narayanpatna goats are medium in size. These 0.57, 70.86 ± 0.60, 73.14 ± 0.73, 16.34 ± 0.12, are mostly brown black in colour. However, 11.49 ± 0.46, 13.96 ± 0.24, and 13.96 ± 0.22 cm, brown, off white and even mixed coloured respectively. The average body weights of adult animal were also seen. These goats are reared by female and male animal were 27.33 ± 0.61 and poor tribal (Kondhs) people for meat purpose 32.80 ± 6.82 kg respectively. under extensive management without any supplementation of feed and fodder. The flock size varied from 2 to 34. Breeding is through natural service. Female animals show sexual maturity at about 12-15 months of age. The average body length (BL), height at withers (BH), chest girth (CG), paunch girth(PG), face length (FL), horn length (HL), ear length (EL) and tail length (TL) in adult male are 74.40 ± 2.77, 69.60 ± 3.71 , 70.60 ± 4.39, 71.40 ± 4.07, 16.00 ± Narayanpatna goat
Table: Average body measurements (cm) and body weights (kg) of Raigari goats
Age Group Sex Height Body Chest Paunch Face Horn Ear Tail Body wt. (No.) at length Girth Girth Length length length length Wither (cm) (cm) (cm) (cm) (cm) (cm) (cm) (cm) Female 54.00 52.00 51.40 51.40 12.20 2.00 13.00 12.40 11.40 (5) ±1.26 ±1.48 ±1.60 ±1.50 ±0.37 ±0.55 ±0.55 ±0.60 ±0.40 Male 54.17 50.33 51.50 54.33 12.00 4.33 13.00 12.17 11.50 3 months (6) ±1.68 ±1.99 ±1.20 ±1.98 ±0.37 ±0.56 ±0.52 ±0.70 ±0.67 Overall 54.09 51.09 51.45 53.00 12.09 3.27 13.00 12.27 11.45 ±1.03 ±1.25 ±0.93 ±1.30 ±0.25 ±0.52 ±0.36 ±0.45 ±0.39
Female 57.60 54.60 57.60 59.00 13.40 4.80 13.00 12.60 15.00 O N O N AND C NSERVATI PHEN O TYPIC CHARACTERIZATI (5) ±1.91 ±1.99 ±2.25 ±1.67 ±0.60 ±0.58 ±0.32 ±0.51 ±0.45 Male 58.60 55.00 58.80 60.80 14.20 6.00 13.40 12.60 16.20 6 months (5) ±0.81 ±1.30 ±0.92 ±1.53 ±0.58 ±0.63 ±0.51 ±0.68 ±0.49 Overall 58.10 54.80 58.20 59.90 13.80 5.40 13.20 12.60 15.60 ±0.99 ±1.12 ±1.16 ±1.11 ±0.42 ±0.45 ±0.29 ±0.40 ±0.37 Female 62.00 60.20 60.60 60.20 13.80 5.20 13.80 13.40 17.60 (5) ±2.17 ±1.91 ±2.04 ±1.20 ±0.37 ±0.49 ±0.58 ±1.12 ±1.08 Male 65.20 61.00 63.00 61.80 14.40 6.20 14.20 14.80 19.60 9 months (5) ±1.07 ±0.63 ±1.38 ±2.08 ±0.40 ±0.73 ±0.37 ±0.20 ±0.81 Overall 63.60 60.60 61.80 61.00 14.10 5.70 14.00 14.10 18.60 ±1.26 ±0.96 ±1.23 ±1.16 ±0.28 ±0.45 ±0.33 ±0.59 ±0.72 Female 68.47 65.63 70.86 73.14 16.34 11.49 13.96 13.96 27.33 (73) ±0.56 ±0.57 ±0.60 ±0.73 ±0.12 ±0.46 ±0.24 ±0.22 ±0.61 Male 74.40 69.60 70.60 71.40 16.00 9.60 15.20 16.20 32.80 Adult (5) ±2.77 ±3.71 ±4.39 ±4.07 ±1.05 ±2.38 ±0.86 ±0.37 ±6.82
Overall 68.85 65.88 70.85 73.03 16.32 11.37 14.04 14.10 27.68 ANNUAL REPORT 2012-2013 ±0.57 ±0.58 ±0.62 ±0.72 ±0.13 ±0.45 ±0.24 ±0.22 ±0.71 Figure in parentheses indicate number of observations 2012-13 19 NBAGR
ANNUAL REPORT 2012-2013 20 PHENOTYPIC CHARACTERIZATION AND CONSERVATION 0.47±0.04, 76.97±1.70respectivel index, yolk index and haugh units are 0.07±0.00, colour. Average weight egg is Albumen 36.5g. Eggs are in size, small mostly light brown in Annualcocks. production egg is about 40-50. weight isBody about 1kginhens and 1.5kgin is generally Earlobe type. white, sometimes red. Comb is red; mainly single, some birds have rose Majority have brown feathers on neck and wings. is black. Pure black are cocks few innumbers. are of Harringhata Black Plumage type. colour West About Bengal. 15-20%of birds flock ineach adjoining areas of North 24Parganas in district from chicken populationLocal was cheractrised Harringhata Black chicken NBAGR Resear is light brown percentage with small of brown with leaner built. The predominant coat colour The donkeys of Sindhi breeds are size of smaller and ploughing. extensively They are used tracts. also carting in about 100kgof as back pack load evenon sandy household. The donkeysSindhi are able carry to region.this About 1-6donkeys are kept per to feed, fodderand water scarcity, endemic to ofdistricts Rajasthan. They are well adapted and Bhil Communities and inBarmer Jaisalmer Sindhi donkeys are reared by Kumhar, Sansi Sindhi donkey Harringhata Block of Nad and district ia 2012- Male Sindhi donkey ch Accomplishents y. showing declining trend. mechanization,increased population their is animals. With better road development and significant differences and inmale female circumference and hoof circumference showed canon length, canon circumference, pastern however some of limb the parameters especially parameters and male between female animals, were not found inmost of morphometric the animals.the Although, significant differences distinguishable and of darker colour inmost of female animals, respectively.tail The switch is of 52.1±4.42and and 51.14 ±4.56cminmale extendstail slightly beyond hocks having lengths 19.0±1.38 and 27.33±1.62cm,respectively. The Whereas, infemales corresponding the values are are 19.7±1.42and 28.39±1.51cm,respectively. lengths of fore and hind limbs animals inmale and respectively. 89.54±14.57kg, Thecanon andmale female animals are 84.95±10.12 The estimated weights of adult (above 3 years) and5.35 inmales 106.52±5.97cminfemales. from 82to 105cm.The chestgirths are 104.3± ± 4.9cm,respectively. lengthbody varies The and female animals are 98.8±3.9and 97.93 slightly concave. The height withersat of male is slightly convex.bone The nasal is straight to andin male female ,respectively. The forehead average length of 46.5±3.22and 45.77±3.1cm, colour. The face is longer thinnerwithand an usually of darker shade than rest the of body the animals. Themanes small are presentwhich are are generally of lighter shade or white inmost legs, ventral sideof neck and inner sidesof ears and animals. grey belly, The inner surfaces of of epididymis. The motility sperms of extracted 2-3 hours from epididymis, cutting caudal region and at sperms extracted room temperature within Goat testis were from collected slaughter house spermatozoa for cryopreservation Utilization of caprine -epididymal cauda 13 jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR was around 75% and live sperm proportion was poultry and cattle, the superior selected breeding 90%. The sperms so obtained were diluted in five males were distributed in the region. A total of different extenders having varying proportion of 20 elite breeding bucks and 3 elite breeding bulls glycerol (4%, 7%, 10% and 14%) to see the effect were procured and distributed to the beneficiaries on post thaw motility parameters and finalize the in Indervally, Kerameri and Beemini mandal. optimum level of glycerol to be used in further experiments. It was observed that 7% glycerol The bulls were distributed to the beneficiaries was most optimum in maintaining the motility, through Biodiversity Management Committee viability, acrosomal and membrane integrity of and bull register is maintained to access the sperm and hence subsequently this level was impact. A total of 170 chicks (Aseel type used in all extenders. An extender containing chicken) of 1-2 weeks old were distributed to 17 trehalose, 20% egg yolk and 7% glycerol could beneficiaries of Keramerimandal (Jhari and Modi retain the maximum motility (65%) after 60 villages). Eighty two kids born were recorded in minutes at room temperature out of seven farmer’s flocks where bucks were supplied in different extenders tried. The sperms diluted in different extenders were frozen through Bheemini mandal. The average body weight of gradient cooling in a programmable freezer kids at 5-6 months of age is 14.16±0.17kg. and after 28 days of storage in liquid nitrogen sperms extended in trehalose, 20% egg yolk Ex situ conservation and 7% glycerol showed maximum post thaw A total of 14,150 frozen semen doses of 10 cattle motility (55%). The viability, acrosomal integrity and membrane integrity was 72, 71 and 60% breeds and 1 buffalo breed were procured and respectively. added to repository in Gene Bank during the year (table). The National Gene Bank at NBAGR Breeding intervention for harmonizing now stores about 1,09,223 frozen semen doses biodiversity (NAIP Project) belonging to thirty eight breeds of seven species (Cattle, Buffalo, Goat, Sheep, Camel, Equine
To upgrade the local germplasm with the O N O N AND C NSERVATI PHEN O TYPIC CHARACTERIZATI intervention of selective breeding in goats, and Yak).
Table : Semen doses added in Gene Bank during the year 2012-13 Species Breeds Number of bulls Semen doses Red Sindhi 2 2000 Cattle Tharparkar 2 2250 Sahiwal 1 1000 Amritmahal 1 500 Dangi 1 500 Hallikar 1 500 Red Kandhari 2 1000 Gangatiri 2 1000 Frieswal 5 2450
Khillar 3 1450 ANNUAL REPORT 2012-2013 Buffalo Banni 3 1500 Total 23 14150 2012-13 21 NBAGR
ANNUAL REPORT 2012-2013 22 GENETIC CHARACTERIZATION and functional genomics NBAGR Resear mitochondrial DNA sequences were obtained. outcarried for and Arunachali yak atotal of 37 amplification of mitochondrial DNA was and cattle-specific microsatellite PCRalleles. through analysing cattle mtDNA sequences in domestic populations yak was assessed make-up of domestic Thecattle yak. admixture flow Gene between yak andcattle affects genetic geographicalthe region rearing populations. yak commonhighly and widely throughout practiced Hybridization and yak between indicine cattle is populations introgression inthedomestic yak Assessment of cattle genetic heterozygosityexpected except Gir. heterozygosity observed the was less than the breeds, respectively. Among three populations, 0.755 and 0.714for Gir, and Sahiwal Tharparkar heterozygosityand 0.664; expected was 0.669, heterozygosityThe observed was 0.675,0.574 (3.655)andSahiwal least inGir (0.661). The number of private highest in also wasalleles Graphic representation of Allelic Patterns across three cattle populations (Pop1-Gir; Pop2-Sahiwal; Pop3-Tharparkar three populations using 84microsatellite The data for patterns allelic across the Gir and HarianaSahiwal, cattle Development of signature breed for functional genomics Genetic Characterizationand Na=Observed numberNa=Observed of alleles, Ne=Effective number of alleles, heterozygosity,He=Expected I=Shannon’s 2012- ch Accomplishents information index) information primers inHimachali Yak. were than higher 4.0for microsatellite the all found number ofpolymorphic. alliles Observed amplified in Himachali Yak markersall and were ETH152TGLA57, and were TGLA73 also ILSTS008, ETH225, TGLA53, TGLA122, CSSM066, MGTG4B, BM2113,ILSTS028, primers MGTG7, viz. AGLA293, TGLA126, 0.167. Total microsatellite 14cattle specific haplotypic ranging frequency from 0.056to 15haplotypesrevealed with inArunachaliyak The mitochondrial DNA analysis sequence in Sahiwal (4.898) andin Sahiwal minimum inGir (3.347). The effective numberalleles was of maximum lowest (11.845)while, in Sahiwal inGir (7.464). below. The mean number wasalleles highest of heterozygosity are presented graphically in figure number of private alleles, and alleles, expected analyzed. Mean number of effective alleles, distributedloci on chromosomes the all was was found 70.79and to be that of unsaturated saturated fatty acids goat inthe samples milk acid methyl ester (FAME).percentage The of from 0.002to 100gram of 41.70gram per Fatty (GC). The value of different fatty acids varied goats was done through gas chromatography Fatty acidprofilingsamples of milk from 500 (DBT Project) quality traits formilk healthfulness Indian goats and their with association Identification of SNPs in QTL in region 13 jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR fatty acid was 28.89 in which monounsaturated isomers cis9 trans11 and trans 10 cis 12 varied fatty acids (MUFA) contribute 24.13% and from 0.006 to 7.88 and from 0.002 to 3.72 mg polyunsaturated fatty acids (PUFA) contribute per gram of milk fat, with an average value of 5.12%. GC estimates of both the isomers (cis9t11 2.41 and 1.02, respectively. GC estimates of both and t10c12) were generally lower than that of the isomers (cis9t11 and t10c12) were generally total conjugated linoleic acid (CLA) estimated lower than that of total CLA estimated through through spectrophotometer. The values of CLA spectrophotometer. Table: Composition of goat milk
Milk constituents (percent) Average (%) Range (%) Fat 4.5 2.06-7.97 Protein 4.18 1.84-5.66 Lactose 6.02 2.62-8.20 SNF 11.06 4.86-15.07
Development of human tissue Study of the genetic variability in the plasminogen activator gene construct bovine cytokines The exon-1, exon-2 and exon-3, 5’UTR and Promoter and regulatory elements of ovine beta- their flanking regions comprising approximately lactoglobulin (oBLG) gene was taken to drive the 3.4 Kb of the IFN-γ gene was amplified and expression of human tissue plasminogen activator sequenced in 40 Zebu cattle representing 5 (tPA) in a mammary gland specific manner. A breeds viz. Tharparkar, Rathi, Sahiwal, Hariana 2.5 kb fragment of oBLG, corresponding to 3’- and Kankrej. A total of 20 SNPs (10 transitions, 8 UTR, containing part of transcription unit and transversions and 2 indels) were observed, spread polyA tail was amplified using Expand Long across the 3.4 Kb region of the bovine IFN-γ gene. The SNP (T>G) at position +176 pposition Template PCR System. The amplified fragment in exon-1 resulted in amino acid change from was subsequently sequenced to check mutation glycine to valine in the signal peptide of the error. Similarly, a fragment of 3.4 kb 5’UTR molecule. This amino acid has been reported including part of transcription unit of oBLG was to be associated with the susceptibility to also amplified. paratuberculosis infection in the Bos taurus O N and functi o nal gen mics CHARACTERIZATI GENETIC populations. The exon-2 and exon-3 of the IFN-γ were fond to be conserved. The microsatellite repeat (GTTT)n in the intron-1 of the gene exhibited a very high degree of polymorphism. The promoter region of the IFN gamma was found to contain transcription factor binding sites for the FOXJ2 and Evi-1 transcription factors at positions c.1672 and c.1697.
Using gene specific primers, the 3.5Kb region of the lymphotoxin part of the TNF β gene, which is closely linked to TNF-alpha gene and the promoter region and exon-1 of the TNF-alpha ANNUAL REPORT 2012-2013
Amplification of 5’ and 3’ UTR of ovine BLG gene. M- gene were amplified in 40 Zebu cattle of Sahiwal, 1Kbp DNA ladder. Hariana, Tharparkar, Rathi and Kankrej breeds. 2012-13 23 NBAGR
ANNUAL REPORT 2012-2013 24 GENETIC CHARACTERIZATION and functional genomics was observed. The Fis was observed. single haplotype block and no recombination Weinberg equilibrium. belonged They to a of SNPs the all except 156(T/G)were inHardy were strongly to linked other. each The genotypes to 10genotypes the The were SNPs observed. probable haplotypes of gene the corresponding TNF-beta gene in indicine cattle. Eight, most at position 185 and Pax-4 at position 531of the transcription factor binding sites for viz. Cre-1 lymphotoxin gene. The region studied twohad acrosswere promoter the observed region of the 360(G>C), 750(A>G),794(A>C)and 851(C>T) 6SNPsOverall at positions 156(T>G),343(G>C), NBAGR Resear gene expression. TNF gene promoter region, for effect their on indigenousthe cattle breeds with to respect their for used could be genotyping and characterizing promoter region of cattle. Therefore, these SNPs previously reported inTNF-beta (lymphotoxin) indicus Bos heterozygosity. expected the were These all heterozygosity forwas greater loci the all than were loci the All polymorphic. The observed excess atand loci low these or no inbreeding. were negative indicated which aheterozygosity promoter in the loci region of TNF-β gene 2012- Differental gene expereries in PMN Differentalacrosscells -7,0 anddays +7 expereries period gene during peripartum specific SNPs specific that had not been ch Accomplishents values at SNP the all HSP70 expression in PMNcells MPAK TNF- signaling, β, NF-κb pathways that pathways inflammatory viz; cytokines,response, The microarray several analysisbased revealed calving, day of calving and 1week calving. post changes across were 1week prior observed to How peripartumthe period. differentially expressed genes (DEG)throughout nuclear (PMN)cells identifiedseveral hundred Transcriptome analysis ofpolymorpho buffalo cattle and buffaloes. inKFcomparisonwas observed to Sahiwal animals. However, physiological higher stress of dairy types inall period peri-partum the presencethe of physiological stress during GGT, BHBA FFA/ NEFA, ROS levels) indicated parameters (complete counts, blood AST, ALT, biochemical Several peri-parturient period. Karan Fries (KF) cows was analysed during adaptation of Murrah Sahiwal buffaloes, and tive physiological and transcriptional Compara and cattle immune suppressioninduced in buffalo toPMN cells parturition- characterize Transcriptome analysis of circulating 13 ever substantial jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
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Identification of suitable ICG in PMN cells being significantly affected. A panel of three of Indian cattle, a total of 23 individuals internal control genes (ICG) viz., RSP9, RPS15A representing 7 native cattle breeds adapted to and RPS23 were identified as most appropriate different agro-climatic regions viz; Amritmahal, for normalization of PMN transcriptional data Gir, Ongole, Red Kandhari, Sahiwal, Tharparkar, during the peripartum period. Comparative Leh cattle and two exotic breeds viz; Holstein and expression analysis of immune related, oxidative Jersey cattle were genotyped using 770K high stress and HSPs revealed Sahiwal to be more density bovine SNP chip. The native cattle from resistant to physiological stress during the Leh and Laddakh region were genetically distinct peripartum period. from the rest of the Indian cattle. This study Genome wide SNP based assessment represents a first approach to assess population of genetic relationship of Indian native structure of Indian native cattle breeds using cattle adapted to different agroclimatic the high density SNP chip and re-establishes condition the genetic distinctness of indicine cattle from taurine cattle. In order to discover the genome wide SNPs and
unravel whole genome based genetic structure O N and functi o nal gen mics CHARACTERIZATI GENETIC ANNUAL REPORT 2012-2013
Multi-dimensional scaling plot revealing genetic relationship between Indian native and Taurine cattle 2012-13 25 NBAGR
ANNUAL REPORT 2012-2013 26 GENETIC CHARACTERIZATION and functional genomics adaptation and exhibit signs of greater distress Riverine have buffaloes less physiological Fellow Project) (National inbuffalo epithelial cells transcriptional response of mammary Impact of heat stress and on cellular (Holstein Friesian cows), response of different taurus cattle Bos types; were to utilized unravel comparative heat shock Peripheral mononuclear blood cells (PBMCs) cattle and buffaloes Differential response heat in shock NBAGR Resear (~54.0 fold). Similarly, HSP60 followed by Holstein (~65.0fold) and Sahiwal to highest the level (~73.0 in buffaloes fold), Comparatively at 2h point,time HSP70 peaked heat stressed PBMCs in cattle and buffaloes. magnitudethe of transcriptional response of and heat shock protein family HSP27 viz; , The quantification of different members of bubalis cows); and Bubalus cellular tolerance to withstand heat stress. as well as buffaloes suggestingtheir differential of transcriptional differences in PBMCs cattleof mRNA after heat stress showedevidence initial at point. time this The higher abundance of HSP transcripts were maximally induced inbuffalo HSP70 out was carried to understand 2012- ch Accomplishents Flow cytometric analysis of cytometric heatFlow stressed buffalo mammaryepithelial cells Bos indicus Bos (Murrah buffaloes). and and (Sahiwal (Sahiwal HSP60, HSP27 upregulated. HSPD1, HSPD8, DNAJA2, DNAJB11 etc. were HSP90AA1, HSP90AB1, HSPA2, HSPA5, heat shock proteins like HSPA1A, HSP90B1, treatment incomparison to control. Most of the in expression at differentpointstime of heat probe displayed sets 2-fold or more changes microarray expression chip. Atotal of 3756 stress24h post samples using Agilent bovine samples were compared with 8h, 12h and well. For transcriptome analysis, unstressed the and remained upregulated at later the stages, as induction expression intheir after heat shock stress conditions. HSPs the All genes, (Bax HSP90HSP70) and and apoptotic/ pro-apoptotic of heat shock proteins (HSP27, HSP40, HSP60, cytometry. At transcriptional level, expression death, cellular apoptosis were by assessed flow proliferation assay, (MTT) cellular viability, cell lactateviz. dehydrogenase activity (LDH), cell cellular andSeveral transcriptional parameters atboth cellular and transcriptional level. in-vitro to model evaluate heat stress response epithelialmammary cells (MECs)were as taken to direct heat exposed stress.when The buffalo BCl 13 2 ) were examined under heat showed immediate jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
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Transcriptional variation of heat shock and apoptotic genes in heat stressed buffalo mammary epithelial cells
Complete transcriptome signature of 120 (mid) and 240 (late) days of lactation was milk derived mammary epithelial cells compared using Agilent 44K microarray chip. (MEC) of Sahiwal cows (NAIP Project) Genes associated with caseins and milk fat synthesis pathways were expressed more during Transcriptional profiling of mammary epithelial early lactation stages. cells (MEC) in Sahiwal cows at 15 (early),
Transcriptome profile of Sahiwal mammary epithelial cells during different lactation stages O N and functi o nal gen mics CHARACTERIZATI GENETIC
Identification of genes expressed in buffalo mammary gland during involution stage (NAIP Project) Transcriptome profile of buffalo mammary gland across different physiological stages was compared in a total of 10 mammary tissues, three each from heifer and involution stages and four from lactating stage to identify stage specific genes. During involution, several genes related to apoptosis, immune and oxidative stress were found to be up-regulated while genes associated with milk fat synthesis, milk protein synthesis were down ANNUAL REPORT 2012-2013 regulated in involution comparison to lactation and Differential gene expressions in buffalo mammary gland heifer stages of buffalo mammary gland. across different physiological stages. 2012-13 27 NBAGR
ANNUAL REPORT 2012-2013 28 GENETIC CHARACTERIZATION and functional genomics NBAGR Resear cellular component and functions. molecular and 21901 entries from biological processes, The gene ontology analysis 21718,22805 revealed assembly had 30053 hits inNCBI -nrdatabase. with GC contents oftranscriptome 50.75%.The software. meanThe contig length was 1815.79 form agoat de-novo transcriptome using CAP3 tissues were merged then with one another to Assembler. The contigs obtainedfrom the ten for 10tissues separately using Trinity De-novo reads. The de-novo assembly was generated first developed using Illumina 2X75bp paired end De-novo assembly of goat transcriptome was (NAIP Project) De-novo assembly of goat transcriptome inbuffalo MEC. period significantlybe involved earlyduring lactation involved production milk inthe were found to Additionally,period. anumber of pathways regulated in buffalo MEC earlyduring lactation upregulated only while 5 genes were down lactation. Atotal 65genes were found to be cow epithelial mammary cells during early in expression profile of and buffalo Sahiwal comprehensive analysis to reveal key The transcriptome data was subjected to (NAIP Project) transcriptome data in cattle and buffalo Pathway analysis of ea Transcriptome profile of buffalo mammarygland across different physiological stages (lactation, 2012- involution, and heifer) involution, ch Accomplishents rly lactation stage differences
The assembly analysis 3605entries to revealed Venn diagram of annotation of goat de-novo Gene ontology (CellularGene component) for Gene ontology (Biological Gene Process) for de-novo contigs in goats goat de-novo contigs 13 transcriptome jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
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Gene ontology (molecular function) for de-novo contigs in goats have at least one enzyme hit, with 21523 entries The expression profile using the junction.bed files identified to have at least one domain. revealed 1348 expressed protein coding genes to be compared among all the breeds. In addition, Differential expression of genes across a number of micro-RNAs found to be up- goats of different geographical locations regulated and down-regulated were discerned. (NAIP Project) The pair-wise comparison of different breeds of goat revealed a large number of genes to be Transcriptome data of 76 bp, paired end reads up-regulated in Sirohi Vs Black Bengal while the on 38 various tissues was generated for the largest number of genes down-regulated were for comparison in the five biological groups, Kanniadu Vs Sirohi (911 genes). The maximum represented by five breeds (Kanniadu, Black down regulation of ncRNA genes was 185 for Bengal, Sirohi, Osmanabadi and Changthangi) Kanniadu Vs Sirohi breeds of goats. The number belonging to different geographical locations. of micro-RNAs being down regulated was also The reads were mapped to the cattle annotated the highest in this set of comparison. The number genome using TopHat software utilizing standard of genes measured, up and down regulated are annotations of the cattle genome. Within each graphically depicted below for skin tissue.
tissue the comparison was made between O N and functi o nal gen mics CHARACTERIZATI GENETIC different geographical regions so as to find out which of the protein coding genes, non- coding mRNAs and micro-RNAs were being up-regulated.
Since the mapping of the raw reads was carried out on the genome, in addition to protein coding mRNA sequences, 184 non-coding RNAs (ncRNAs) as well as micro-RNAs were also mapped. The comparison of expression level for each of the mapped gene and the expression occurring in exon or alternative splice junction was also carried out. For each tissue ten ANNUAL REPORT 2012-2013 comparisons were made involving two different Differential expression of genes in pair-wise comparisons in goats breeds each time. 2012-13 29 NBAGR
ANNUAL REPORT 2012-2013 30 GENETIC CHARACTERIZATION and functional genomics NBAGR Resear entries identified to have at least one domain. A entries to have at least one enzyme hit with 21514 functions. The assembly analysis 3226 revealed processes, cellular components and molecular 24676, 26027and 24949entries from biological nr-database. The gene ontology analysis revealed transcriptome assembly had 32940hits inNCBI was 1311.03with GCcontents of 51.24%.The using CAP3software. meanThe contig length another to form acamel de-novo transcriptome from ten the tissues were merged then with one Trinity De-novo assembler. The contigs obtained generated first fortissuesseparately 10 using bp paired end reads. The de-novo assembly was transcriptome was genrated using Illumina 2x75 De-novo assembly of dromedarian camel transcriptome (NAIP Project) De-novo Venn diagram of annotation of dromedarian camel de- Gene ontology ofGene the differentiallyexpressed genes 2012- novo transcriptome novo assembly of camel ch Accomplishents for 9tissues the of dromedarian camel. total accessions of 5.81614lakh were obtained (marker 5),and chromosome 5(marker 2and 3). with chromosome 1(marker1), chromosome 3 5. Minor QTLs were found also associated to be chromosome 4and marker 11of chromosome 2, marker 5 of chromosome 3, marker 9 of 8 of chromosome 1,marker 7of chromosome significant effect were associated with marker deviation of 57.29. Major highly QTL with very was found 132.9414Kgwith astandard to be analysed collectively. The body meanweight received for 12months of age. Thedata was A total of 1946records weights Abody were marker 10on chromosome 8. chromosome 6,marker 9on chromosome 7and marker 11on chromosome 5,marker 10on chromosome 3,marker 12on chromosome 4, 1, marker 2on chromosome 8,marker 9on markersthe were marker 10 on chromosome eight chromosomes. The QTLs associatedwith QTL for trait. this The analysis wascarried out for identification of markers thewithassociated 112.2063Kg Thedatato be was analysed forthe twelve sires. Thebody meanweight was found were received frombelongedwhich field the to A total of 509 records of weight body of heifers QTL mapping. weights indifferent was collected agegroups for 10-15 cM on eachchromosome. Data on body microsatellite markers with agenetic distance of chromosomes have genotyped using been five chromosomes. Thus eight inall buffalo in addition to genotyping the out carried in correct paternity. This genotyping was done in 8400buffalodaughters the 12siresof with genome database were and selected amplified Total 30microsatellite from loci cattle the (NAIP Project) Identification of QTL for in buffaloes 13 jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
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A total of 2789 records were received for 18 Buffalo and goat had highly variable region months of age. The data was analysed collectively. from 724 nucleotides upwards to first exon in The mean body weight was found to be 152.754 comparison to cattle. To detect polymorphism Kg. Major QTL with very highly significant effect in 5’-upstream of TLR8 gene, eight samples of were associated with marker 2 of chromosome swamp and riverine buffaloes were sequenced, 2, marker 6,7,9 of chromosome 3, marker 8 of which revealed two polymorphic sites, at 139 chromosome 4, marker 3 of chromosome 6 and nucleotides and 128 nucleotides upstream to marker 6 of chromosome 7 and marker 8 of the ORF. A PCR-RFLP genotyping assay was chromosome 8. developed for the g-139G>T SNP using Tsp509I restriction enzyme to screen 710 samples of A total of 2936 records were received for 24 Indian buffalo breeds including both swamp and months of age. The data was analysed collectively riverine types from different agro-climatic zones for all the daughters. The mean body weight and allelic distribution varied across swamp and was found to be 196.5954 Kg. Major QTL with riverine buffaloes. Besides this, most variable very highly significant effect were associated ectodomain regions of TLR2 and TLR4 genes with marker 1 and 9 of chromosome 3, marker were genotyped by sequencing in 112 animals 1,7,9,12 of chromosome 4. Minor QTLs were also representing Murrah, Toda and Swamp types, detected for marker 8 each of chromosome no. which showed seven haplotypes in TLR4, one 2 and chromosome no. 3. haplotype with a frequency of 25.9% being A total of 2650 records were received for 30 specific to Murrah and another major haplotype months of age. The data was analysed collectively having 69.6% frequency was shared by Toda and for all the daughters. The mean body weight was swamp buffaloes. Two animals each representing found to be 218.9249 Kg. Major QTL with very one of the four haplotypes observed in TLR4 highly significant effect were associated with were screened for expression variation after marker 8 of chromosome 4. Minor QTLs were lipopolysaccharides (LPS) ligand stimulation also detected for marker 8 each of chromosome of neutrophils purified from blood. There was no. 2 and chromosome no. 3. higher expression of TLR4 at four hours post stimulation, whereas cytokines expression A total of 1394 records were received for 36 increased 8 hours post stimulation. Somatic cells O N and functi o nal gen mics CHARACTERIZATI GENETIC months of age. The data was analysed collectively purified from milk and neutrophils from blood for all the daughters. The mean body weight was samples of mastitis affected and non-affected found to be 243.8293 Kg. Major QTL with very Murrah animals showed higher expression of highly significant effect were associated with pro-inflammatory cytokines in mastitis animals. marker 3 of chromosome 7. Minor QTLs were In another study, polymorphism detected in goat also detected for marker 8 each of chromosome TLR7 gene was further investigated for impact no. 2, marker 1 of chromosome no. 4 and marker on gene structure and function using Polyphen 5 of chromosome no. 7. 2 programme and one of the possibly damaging amino acid substitutions, 127 Thr>Met has Characterization of Toll-like receptor been identified. Overall, these results helped (TLR) genes in farm animals (NAIP in identification of genetic diversity among Project)
indigenous livestock populations, which could ANNUAL REPORT 2012-2013 More than 1 kb nucleotides long 5’-upstream be further utilized for association with disease region of TLR8 gene of buffalo, cattle and resistance traits. goat was amplified, sequenced and compared. 2012-13 31 NBAGR
ANNUAL REPORT 2012-2013 32 GENETIC CHARACTERIZATION and functional genomics NBAGR Resear analysis of various regions including 5’ upstream, through sequencing of genomic region. Sequence was characterized inbuffalo, cattle and yak Haptoglobin (Hp) gene associated with mastitis cattle and buffaloes in dairy of incidence with mastitisassociated identification variants of allelic Candidate gene analysis and B5724 B478 377 C>T Nucleotide B479 Haplotype 845T>C 130 G>A 1177T>C 1340T>G 2548 A>G 2802 T>C Polyphen analysis of goat TLR7polymorphism showing impact of non-synonymous SNPs on its and structure 2012- Expression analysis of TLR4and in LPSligand cytokines stimulated neutrophils of Murrah Polymorphism in buffalo detection TLR8 promoter at -139G>T by Tsp509I PCR-RFLP 127 Thr>Met* Amino acid 282 Leu>Pro 44 Ala>Thr* 393 Cys>Arg 448 Met>Arg 850 Ile>Val 935Leu>Ser 935Leu>Ser 8hr . 8hr . 4hr . 8hr . 4hr . 4hr . stimulation post interval Time ch Accomplishents function. (*indicatesfunction. amino having acid possibly damaging effect) 2.25 1.27 1.64 105.79 7.59 24.59 TLR4 Transition Transversion Transition/ Transition Transition Transition Transversion Transition Transition buffalo representing three haplotypes 2.22 91.46 11.27 1.87 41.50 4.98 CXCR2 LRR Domain LRRNT LRR Region Low Complexity TIR Domain Fold change inexpression Buffalo haptoglobin showed molecule unique from riverinegland buffalotissue. mammary Haptoglobin cDNA was amplified sequenced and compared to cattle. Further, a1329bp long 35 nucleotide changes in CDS, 5 in 5’UTR indels. haptoglobin Buffalo gene revealed large number of nucleotide variations including exonic and intronic regions of gene the showed a 37.66 5.08 14.72 7.46 1.74 8.22 PGLY Effect Effect changeof amino acid 13 66.26 62.03 103.61 47.18 3.47 2.08 TNF 93.70 54.95 14.47 7.89 6.75 5.45 IL6 jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR characteristics as internal duplication of large PGLYRP1 were not found to have significant coding region compared to other non ruminant difference of allele frequencies between mastitis species. Phylogenetic tree of haptoglobin showed affected and healthy buffalo. Variable numbers clustering of all bovine species along with (12/13) of glycine residues in cattle FEZL gene human, revealing convergent evolution having responsible for resistance/susceptibility to taken place between ruminants and human. mastitis was also found in buffaloes, however, About 330 bp and 400 bp long buffalo S100A8 without any significant association with mastitis and SAA3 cDNA molecules, respectively were in buffalo. Expression of SAA3, Hp, PGLYRP1 amplified, covering complete CDS. Buffalo and CCL2 molecules was also assessed in healthy S100A8 cDNA had 96, 94 and 86% identities and mastitis affected cattle through real time- with that of cattle, sheep and pig sequences, PCR. Higher expression of all the four proteins respectively. The buffalo SAA3 showed 96, 95 was observed in acutephase mastitis compared and 94% nucleotide identities with that of cattle, to healthy cattle. goat and sheep sequences. Phylogenetic analysis of CDS and predicted amino acid sequences of Delineating polymorphism and SAA3 and S100A8 revealed clustering of cattle, evolution of Toll like receptors in yak and buffalo species in same node, overall. Indian native (Bos indicus) cattle breeds Other non ruminant species clustered separately Sequence characterization and genotyping of away from these three species. eight Toll like receptor (TLR 1, 2,4,5,6,8,9 and10 SNPs identified in TNFα, haptoglobin and ) genes was undertaken in Indian native cattle PGLYRP1 were studied in a panel of mastitis breeds (Bos indicus) to identify novel single (24) and healthy (24) buffaloes and cattle for nucleotide polymorphisms (SNPs). Comparative association with incidence of mastitis in Murrah sequence analysis of coding region revealed buffalo and Sahiwal cow. SNPs at position 156 SNPs in the TLRs studied. TLR6 was most 136C>A, 153G>T of PGLYRP1 and at 340A>G, diverse with 29 SNPs while TLR9 showed 418T>C of Hp were found to have significantly minimum number (15) of SNPs. Out of 156 (p<0.05) different allelic frequencies between SNPs, 79 were non-synonymous and 51were mastitis and healthy cattle. SNPs at TNF, Hp, novel to the Indian cattle breeds. O N and functi o nal gen mics CHARACTERIZATI GENETIC ANNUAL REPORT 2012-2013
Details for the SNPs across studied Toll like receptor genes (TLRs) in cattle 2012-13 33 NBAGR
ANNUAL REPORT 2012-2013 34 GENETIC CHARACTERIZATION and functional genomics NBAGR Resear Analysis of 79 non-synonymous analysis grouping close revealed taurus of Bos divergence from taurine the counterpart. The sufficient diversity within Indian cattle and study the 1. Overall, suggests existence the of positiveTLRs revealed inTLR4and selection analysis for of studied inthe evidence selection unaffected. seem TLR8 molecules Preliminary protein domain alterations whereas, TLR1and 10 resulted in confident the prediction of replacements in bovine TLR2, 4, 6, 7, 9 and patterning. predicted amino Several acid of conservation in TLR the variable LLR other mammalian revealed regions species TLR gene clusters of Indian native cattle with of protein domain architecture for different were found Comparison conserved. to be Except few, polarities of most of amino acids unbiased and distributed across domains. the all polymorphic nucleotide sites were biallelic, least polymorphic. observed the all Overall, highest number of SNPs and Kangyam the was Hariana and Gir cattle were found to have the Amongst analyzed the breeds, Kankrej, damaging, other SNPs all were benign. region coding in the of TLR6was found to be while onlyeffect one the of nsSNPs observed predicted 12 SNPs having possibly damaging PolyPhen-2 Analysis of amino substitution acid effects in TLR 4; B: Benign; PD:in possibly TLR4;B:Benign; damaging 2012- ch Accomplishents SNPs (nsSNPs)
other species. at analyzed the genes, all compared when with was observed that g.6723G>A the SNPwas observed in the reference It DQ530260). sequence (GenBank exon 3region with 100%homology with the angene revealed absence of loci SNPs inthe The analysis editedthesequences of of GDF8 after were trimming and analysed. aligned in an automated DNA sequencer.sequences, The amplified products were sequenced purified and were to amplify used target the gene The loci. and calpain (CAPN) gene. primers Specific factor 8(GDF8), β3adrenergic receptor (ADRB3) polymorphism at growth the differentiation Muzzafarnagri and Nali were analyzed for genetic Ganjam, Garole, Magra, Madgyal, Malpura, breeds namely Bandur, Deccani, Chokla, A total of 50samples from ten Indian sheep sheep for mutton quality traits in Indian Nucleotide diversity incandidate genes across differentobserved TLRs. was for performed non-synonymous the SNPs protein modeling and structure visualization groupingclose followed by TLR9.Additionally, followed by TLR2.Similarly, TLR7 and 8showed TLR 1,6and 10were grouped inone cluster and and Phylogenetic relationship among TLRs(1-10)from Bos indicus Bos different mammalian different species. (Indian native cattle breeds) 13 jk-i-vk-la-C;wjks
RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR 3’UTR, reported to affect muscularity in exotic Genetic characterization of Spiti donkey sheep, appeared to be fixed for g.6723G allele in the Indian sheep investigated. A total of three The Spiti donkeys distributed in Spiti and SNPs (-41C>A; g.3995G>T) were identified Yangthang regions of Himachal Pradesh were in the non coding regions of GDF8 gene in evaluated for within breed genetic diversity and Indian sheep, out of which -16C>T has not genetic bottlenecks using twelve heterologous been reported previously. Two SNPs identified microsatellite markers. The genomic DNA was in the Indian sheep are known to be associated amplified by PCR using FAM and HEX labeled with mutton quality and carcass traits in exotic primers and resolved for alleles on automated sheep. These initial results showing genetic DNA sequencer. The PCR product size range variability and presence of favourable alleles/ varied from 78-87 bp at locus VHL20 to 253- SNPs in the GDF8 gene may lead to exhaustive 271 bp at locus COR18. The observed number analysis of genetic polymorphism in GDF8 gene of alleles ranged from 4 (VHL20, HTG6) to in Indian sheep breeds. The results also suggest 10 (HTG7), with a mean of 6.33 ± 1.72 alleles potential prospects for genetic improvement of per locus. The effective number of alleles (Ne) Indian sheep through selective breeding. A 900 ranged from 2.03 (VHL209) to 4.84 (HTG7), bp region spanning the 5’UTR and partial exon with a mean value of 3.35 ± 0.94. The observed 1 as well as 245bp of 3’UTR of ovine ADRB3 heterozygosity (Ho) values across the twelve loci gene was screened for possible SNPs in these ranged from 0.48 (NVHEQ54) to 0.89 (HTG7), sheep breeds. The sequence analysis revealed with a mean value of 0.72±0.13. The expected two SNPs in the exonic region (g.333C>T and heterozygosity (He) varied from 0.51 (VHL209) g.497G>C), both of which were synonymous. to 0.80 (HTG7 and AHT4), with a mean of 0.69 No SNPs were detected in the 5’UTR. One SNP ±0.09. The allele number and heterozygosity was also detected in the 3’UTR (g.2621T>C). The values observed across the studied loci indicated identified SNPs were present in the heterozygous presence of reasonably high levels of genetic state. Further, a 190 bp polymorphic region of variability in Spiti donkeys. The mean PIC for ovine CAPN gene covering partial exon 5, intron all loci assessed from allele frequency data was 5 and exon 6 was also sequenced across the panel 0.68±0.09, ranging from 0.51 (VHL209) to 0.79 of ten sheep breeds. A single C>T transversion in (HTG7 and AHT4). These PIC values suggested the heterozygous state was detected in the intron that these loci were sufficiently polymorphic 5. The frequency of the C and T alleles was 0.564 O N and functi o nal gen mics CHARACTERIZATI GENETIC reflecting their suitability for genetic variability and 0.436 respectively. ANNUAL REPORT 2012-2013
Normal ‘L’-shaped curve for allelic distribution in spit donkeys. 2012-13 35 NBAGR
ANNUAL REPORT 2012-2013 36 GENETIC CHARACTERIZATION and functional genomics recent genetic bottlenecks Spiti inthe donkeys. stronglyfrequency indicated absence of any The normal ‘L’ shaped distribution of allelic indicating moderate levels of inbreeding. Dairying and of AgricultureFisheries, Ministry Dairying As Department of per Animal Husbandry, species Cytogenetic of screening livestock mean genetic diversity estimate (F studies of indigenous donkey breeds. The NBAGR Resear any of other chromosomes complement. inthe translocated chromosome was much larger than One bull had a major translocation such that the is depicted infigurebelow. XX) chromosome Sex twin. chimerism (50,XY/ 50, concerned it agency whether wassingle or born XY). Though no record was availablewiththe chromosomewas a sex chimeric (50, XX/50, total bulls investigated. One buffalo calf bull abnormalities, thus constituting 1.18%of the Five bulls were identified to have chromosomal Total 11 10 9 8 7 6 5 4 3 2 1 S. No. 2012- Milkfed Khanna,Milkfed Punjab Moradabad Uttar Pradesh Livestock Development Board, Dalpatpur, Kerala Livestock Development Board, Mattupatty Punjab Livestock Development Board, Nabha Uttar Pradesh Livestock Development Ganjaria Board, Chak Project Directorate on Cattle, Meerut InstituteCentral for Research on Buffaloes, Hisar Himachal Pradesh Livestock Development Board, Palmapur Haryana Livestock Development Board, Gurgaon Haryana Livestock Development Board, Jagadhari Haryana Livestock Development Board, Hisar ch Accomplishents Number of buffalo screened cytogenetically Number screened of buffalo IS Agency ) was 0.048 Y chromosome. This was considered as a metacentric Ychromosome into acrocentric with remaining the thus part transforming the in table below. agencies. The detail screenedof is bulls provided by karyotyping of animals from these various of more generated was than also Rs.2lakhs concernedthe stakeholders. Atotal revenue and screening the results communicated to parameters cytogenetic their during year the A total of 423 breeding bulls were forscreened forused breeding made mandatory. has been screening of cattle all and buffalo be bulls to for Cattle and Breeding, Buffalo cytogenetic guidelines under National the Programme rotation of broken the at part 180 breakagebe of arms small near centromere, the indicus characteristic of Bos two bulls had acrocentric Ychromosome, type 91 such crossbred bulls (Frieswal) investigated oftype Ychromosome (a metacentric). Ofthe indigenous females should have a by mating exotic Holstein Frisian bulls with point of translocation. crossbred All bulls derived Moreover, there appeared aconstriction at the 13 126 10 8 0 83 1 0 4 0 4 0 16 Buffalo cattle. There could 297 10 3 86 63 30 91 1 5 1 3 4 Bulls Cattle 0 and reunion jk-i-vk-la-C;wjks Bos taurus Bos 423 20 11 86 146 31 91 5 5 5 12 20 Total
RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR chromosomal abnormality and the bulls were of chromatid gaps and breaks is observed but culled from the breeding programme. the frequency never exceeds 5% of the cells examined. Counselling was accordingly provided Another cattle bull had a high frequency of to the concerned agencies regarding use of bulls chromatid gaps and breaks (more than 16%). for breeding. While in the normal bulls frequent occurrence
Karyotypes showing sex chromosome chimerism (Female-50, XX (left) and Male- 50, XY (right)) O N and functi o nal gen mics CHARACTERIZATI GENETIC ANNUAL REPORT 2012-2013
2012-13 37 NBAGR
ANNUAL REPORT 2012-2013 38 NETWORK PROJECT on angr the A959Candthe G1189Amutations inGDF9 gene low prolific (Sirohi, Ganjam) werescreened for medium prolific (Sangamneri, Osmanabadi) and Barbari,prolific Jhakrana), Beetal, Bengal, (Black Eight goat breeds differing in viz.high prolificacy GDF9 Laboratory, (Core NBAGR) PCR-RFLP genotyping of SNPs ingoat Network onAngr Project NBAGR Resear the allele distribution allele the at G1189Apolymorphic different prolificacy did not show differences in in other Indian breeds. Indian goats with only two genotypes (GGand GA)were recorded goatfor only. SNP 1189G>AinBeetal Whereas, Three genotypes GG,GA and AA were observed Ganjam Sirohi Osmanabadi Sangamneri Jhakrana Barbari Beetal Black Bengal Breed BstNI PCR-RFLP of frequencies genotype GDF9 SNP 959A>Cindifferent goat breeds 2012- A959C SNP in goat GDF gene PCR-RFLP of genotype ch Accomplishents 50 50 41 50 14 50 28 52 screened Number of animals 36 36 39 42 13 46 27 25 AA indigenous goat. related to G1189A mutation of GDF9 gene in twinning or multiple kidding might not be thatrevealed genetic the factor responsible for non-prolific GanjamResults (G=0.93, A=0.07). prolificboth Bengal (G=0.98,A=0.02) and Black was of frequency Allele loci. similar range for Black Bengal goats.Black Bengal favorable highest (0.27)for was also Callele the Indian breeds. The proportion of withanimals genotypes (AAand AC) were recorded inother in prolificBengal goat,Blackwhereas twoonly three the genotypes:all AA, AC and only CC goats.Chinese Screening of A959CSNP showed known associated to prolificacy with be high in through PCR-RFLP genotyping. A959CSNP is G1189A SNP in goat GDF9 by PCR-RFLP Genotype (No.Genotype of animals) 4 13 2 7 1 4 1 26 AC 13 Genotyping of of Genotyping 0 0 0 0 0 0 0 1 CC jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR
MspI PCR-RFLP genotype frequencies of GDF9 SNP 1189 G>A in different goat breeds
Breed Number of animals Genotypes (Number of animals) screened GG GA AA Black Bengal 52 50 2 0 Beetal 28 72 19 2 Barbari 50 39 10 0 Jhakrana 14 1 13 0 Sangamneri 50 28 22 0 Osmanabadi 41 28 13 0 Sirohi 50 44 6 0 Ganjam 50 43 7 0
Detection of polymorphism in caprine of eight goat breeds-Beetal, Sirohi, Osmanabadi, Malabari, Jakhrana, Barbari, Black Bengal and BMPR1B gene (Core Laboratory, NETW O RK PR JECT o n a gr NBAGR) Ganjam differing in prolificacy, geographical distribution and genetic diversity. Caprine BMPR1B gene was amplified and screened for polymorphism analysis in a panel
Geographical distribution of selected goat breeds Sequence analysis revealed absence of any putative binding sites for the transcription factors polymorphism including the FecB mutation in viz. COMP1, BR-C Z1, HNF-1 and FOXJ-2 were BMPRIB exonic regions of all the investigated identified. Total almost one hundred variations breeds of Indian goats. Coding DNA sequences were observed between cattle and goat promoter (CDS) of caprine BMPR1B gene revealed 97% sequence. Two novel SNPs T(-242)C and G(-623) homology with Bos taurus (BC134547) and 99% A have been identified in the promoter region with Ovis aries (AF357007) and reported Capra of caprine BMPR1B gene, of which all the three hircus (DQ666418). BMPR1B promoter region genotypes (GG, GA and AA) were observed ANNUAL REPORT 2012-2013 was also amplified in two fragments and a 1032 for -623G>A SNP whereas, two genotypes nucletides length contig was generated and four (TT and TC) were observed for -242T>C SNP. 2012-13 39 NBAGR
ANNUAL REPORT 2012-2013 40 NETWORK PROJECT on angr in caprine KiSS1 as compared taurus to Bos Ninety nine nucleotide changes were observed goat breeds differing in precocity and prolificacy. identification of SNPspanel ina of 9 Indian region) was amplified sequenced and forthe intron 1 and exon 2 along with downstream KiSS-1 (kisspeptin) exon gene (5΄UTR, 1, Laboratory,(Core NBAGR) precocity inindigenoussexual goats Characterization of candidate genes for factors binding sites. BMPR1B gene didnot change any transcription Polymorphism promoter in the region of caprine NBAGR Resear indigenous breeds were identified. MCT and 3SNPs inCD147genes to specific and Sindhi. Four SNPs in MSTN, 12 SNPs in equine breeds of India Kathiawari, viz., Marwari targeted to explore SNPs type in three saddle transporters (MCTs) and CD147genes were Myostatin (MSTN), Monocarboxylate Laboratory, Anand) physical performance inhorses (Core Polymorphism incandidate genes for 6 5 4 3 2 1 S. No. 2012- T1125C T950C T693C A505T T455G G296C SNP ch Accomplishents Intron 2 Intron Intron 1 Intron Region Allele of SNPs frequencies in caprine KISS-1gene TC=0.083 TT=0.917 CC=0.021 TC=0.125 TT=0.854 CC=0.05 TC=0.20 TT=0.75 TA=0.575 TT=0.425 GG=0.125 TG=0.550 TT=0.325 CC=0.050 GC=0.275 GG=0.675 Genotype frequency Genotype associated with multiple kidding. T454G and A505T),of G296Chas which been well as three previously SNPs described (G296C, three novel SNPs (T693C,T950C,T1125C)as indigenous goats resulted inidentification of Comparison of 45 amplified sequences of goats and exotic Jining greygoat (GU142847.1). amplifiedbetween sequence KiSS1 of Indian cattle. However, no variation was observed deletions incaprine sequence as compared to (1806-1852 of NW_003104427.7)nucleotides 26 (1417-1442of NW_003104427.7)and 47 at four positions that included continuous at 3positions, whereas deletions were recorded (NW_003104427.7). Insertions were observed horses infour clusters and Marwari inwhich Sindhi Analysis (PCA)showed distribution the of individual Principal ComponentMarwari (0.084)was observed. Kathiawari and Sindhi (0.058) and Sindhi and between Kathiawaribetween and (0.034), Marwari between 11% variation among breeds. FST differentiation studiedloci inthree horse population. AMOVA indicated Total at were 263alleles 25microsatellite the observed Laboratory, Anand) and Sindhi horses (CoreMarwari characterizationGenetic of Kathiawari, 13 C=0.04 T=0.96 C=0.08 T=0.92 C=0.15 T=0.85 A=0.29 T=0.71 G=0.4 T=0.6 C=0.19 G=0.81 Allele frequency Allele jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR horses formed two separate clusters whereas Kathiawari Molecular characterization of pig did not form distinct cluster but overlapped with populations (Core Laboratory AAU, Marwari and Sindhi. Khanapara)
Molecular characterization of Kharai Three indigenous pig populations viz., Votho camel (Core Laboratory, Anand) pig from Nagaland, indigenous pig from Arunachal Pradesh and Mali pig from Tripura Total 224 samples (108 from Kachchhi and of North East India has been studied using 118 from Kharai) with 25 microsatellite loci 21 polymorphic microsatellite loci. Mean revealed FIS for Kachchhi and Kharai breeds 0.197±0.033 and 0.397±0.053 respectively. numbers of observed alleles were 5.591±0.598, 4.909±0.562 and 5.636±0.663; and effective Overall FIT and FST values were 0.336±0.038 and number of alleles were 3.021±0.284, 2.885±0.279 0.044±0.009 respectively. High and positive FIS values indicating strong heterozygote deficiency and 3.165±0.328, in Vatho, Arunachali and Mali within population. Mean FST indicated breed pig populations, respectively. FIS values were differentiation among these breeds which was 0.273±0.079, 0.409±0.066 and 0.290±0.066 in NETW O RK PR JECT o n a gr supported by PCA and STRUCTURE analysis Vatho, Arunachali and Mali pig populations, showing three distinct clusters indicating respectively. that Kharai breed is genetically different from Kachchhi breed.
Votho Pig Arunachali Pig ANNUAL REPORT 2012-2013
Mali Pig 2012-13 41 NBAGR
ANNUAL REPORT 2012-2013 42 NETWORK PROJECT on angr lactate levels were estimated inHallikar and draught power like creatine and kinase blood in cattle: Biochemical parameters to pertaining Analysis of genes associated with draught power laboratory, TANUVAS, Chennai) draught power incattle (Core Analysis of genes with associated NBAGR Resear polymorphisms evaluation. and further needs repeats found 11250to 11290exhibited between found intronic inthe regions. GAmicrosatellite 7877, 8283, 11270, 11272, 13285 and 13287 were incattlesequenced breeds. Six SNPs at positions bp and 712bp. Products were amplified and sizes of 660 bp, 732 bp, 450 bp, 701 bp, 340 with 7primersequenced with product the sets migration. Promoter and exonic regions were through endothelial proliferation cell and factor peripheral circulation (VEGF)affects breeds of cattle. Vascular endothelial growth draughtability inKangayam, Bargur and Hallikar suggest is apotential this locus marker for length. High He values of promoter microsatellite IGF-1 gene was found associated to be with stride among 3cattle breeds; SNP 1(GG,GAand AA)of A total of sixSNPs were found inIGF-1 gene laboratory, TANUVAS, Chennai) gene inindigenous cattle (Core breeds of SNPsGenotypic frequency at ADRB2 1311T>C (SNP 3) 1293C>T (SNP 2) 568 G>A(SNP 1) SNPs 2012- ch Accomplishents Table: Polymorphism across ADRB2 gene inIndian cattle breeds. CC CT TT TT CT CC AA GA GG Genotype 0.21 0.05 0.74 0.56 0.44 0.0 0.0 0.24 0.76 Bargur frequencies Genotype breeds of cattle. (n=130), Bargur (n=100)and Hallikar (n=96) 1311, 1351and were genotyped inKangayam SNPs inADBR2 gene at positions -568,1293, Tetra-ARMS PCRprimers were for designed product sizesof 422,472,661,700and 694bp. withwas sequenced 5primer with the sets other southern breeds of cattle. ADRB2 gene 98.48, respectively. and lambing percentage at farm was 81.48and up toscheme 31/03/2013.The period tupping of have Kilakarsal recorded been during the +797female) 1504(707male progeniesOverall progenies were produced during 2012-13. A total of +242female) 451(209male Kilakarsal Ramayanpatti, Tirunelveli, TANUVAS) Farm (Instructional Livestock Complex, sheep of Kilakarsal Conservation Kilakarsal sheep being reared at Tirunelveli farm 0.26 0.57 0.17 0.57 0.43 0.0 0.0 0.47 0.53 Kangayam 13 0.54 0.25 0.21 0.71 0.27 0.02 0.09 0.89 0.02 Hallikar jk-i-vk-la-C;wjks RNBAGResearch Accomplishments
jk-i-vk-la-C;wjks NBAGR
Table: Average body weights (kg) in Kilakarsal sheep
Sex Birth weight 3 months 6 months 9 months 12 months Male 2.79 ± 0.05 (30) 10.21 ± 0.57 (14) 13.11 ± 0.49 (10) 15.20 ± 0.89 (03) 21.00 ± 1.05 (14) Female 2.64 ± 0.08 (27) 9.27 ± 0.52 (15) 12.94 ± 0.35 (14) 14.15 ± 0.28 (07) 17.04 ± 0.45 (24) Overall 2.72 ± 0.04 (57) 9.71 ± 0.39 (29) 13.00 ± 0.28 (24) 14.40 ± 0.31 (10) 18.57 ± 0.53 (38)
Table: Carcass characteristics in Kilakarsal sheep
Age - 6 months Age – 12 months Male Female Overall Male Female Overall Weight at slaughter (kg) 14.47 ± 0.14 13.79 ± 0.07 14.13 ± 0.13 19.75 ± 0.59 17.27 ± 0.22 18.51 ± 0.48 Carcass weight (kg) 6.82 ± 0.16 6.29 ± 0.04 6.55 ± 0.11 8.80 ± 0.22 7.60 ± 0.16 8.20 ± 0.22 Dressing percentage 47.10 ± 0.70 45.50 ± 0.15 46.33 ± 0.41 44.60 ± 0.60 43.99 ± 0.45 44.33 ± 0.37 NETW O RK PR JECT o n a gr (Values are means of six observations)
Conservation of Krishna Valley conservation of Krishna Valley cattle total cattle (BAIF Development Research 10,228 inseminations were performed. 2,777 Foundation Uruli-Kanchan) pregnancies were followed and recorded average conception rate of 51.55 per cent. So far 725 The project was operational in 25 cattle males and 627 females were born in the breeding development centers and Govt. dispensaries of tract. Along with this 433 pregnant animals were Department of Animal Husbandry Karnataka sold by the owners. state. Since inception of the project on ANNUAL REPORT 2012-2013
2012-13 43 ANNUAL REPORT 2012-2013 44 2012- 13 NBAGR
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Research Projects & Publications
• Research Projects • Publications • Awards/Recognition ANNUAL REPORT 2012-2013
2012-13 45 ANNUAL REPORT 2012-2013 46 2012- 13 RESEARCNBAGRH PROJECTS & PUBLICATIONS
jk-i-vk-la-C;wjks NBAGR Research Projects Singh and DK Sadana - April, 2011 to March, 2014. Completed Research Projects 3. Phenotypic and genetic characterization of 1. Characterization of lesser known goat Koraput sheep. - Sanjeev Singh, KN Raja, populations of Maharashtra and Odisha Reena Arora, Indrajit Ganguli and Sanat states of India- NK Verma, RAK Aggarwal, Mishra, CEO, OLRDS, Bhubaneshwa SP Dixit, PS Dangi, SK Das (OUAT), SV - April, 2012 to March, 2014. Kuralkar (PGI VAS, AKOLA) and VS Kawitkar (DBSKKV, DAPOLI). 4. Classification of ecotypes of Deccani sheep - Dinesh Kumar Yadav, Reen Arora and 2. Phenotypic and genetic characterization RESEARCH PR O JECTS Anand Jain - April, 2012 to March, 2016. of Indigenous donkey population-Rahul Behl, Jyotsna Behl and DK Sadana. (SC 5. Characterization of non-descript goat Gupta up to 08.02.2010, Neelam Gupta up genetic resources of Rohilkhand Region of to 31.07.2010 and KN Raja & BK Joshi up Uttar Pradesh and Uttarakhand. SP Dixit, to 30.06.2011) Triveni Dutt (IVRI), PS Dangi, RS Barwal, Neel Kant (GBPUAT) and Vikas Vohra (w.e.f. 3. Transcriptome analysis of circulating PMN 14.07, 2011)- January 2010 – March 2014. to characterize parturition-induced immune suppression in buffalo and cattle-M Mukesh, 6. Assessment of cattle genetic introgression in M Sodhi, MS Tantia, AP Mohanty (NDRI) the domestic yak populations. -Jayakumar S and Indrajit Ganguli w.e.f. 22.10.2011. (BP and Karan Veer Singh- April, 2012 to March, Mishra up to 24.02. 2011and RS Kataria up 2014 . to 30.06, 2011) 7. Phenotypic characterization of Harrighata 4. Candidate gene analysis and identification Black breed of chicken - PK Vij, MS Tantia of allelic variants associated with incidence and S Pan (WB Univ. Calcutta) - April, 2012 of mastitis in dairy cattle and buffaloes-SK to March, 2014. Niranjan (w.e.f. 26.08.2011), RS Kataria and AK Dang (NDRI) (BP Mishra up to 8. Utilization of cauda epididymal spermatozoa 24.02.2011, BK Joshi up to 06.07.11 and P for cryopreservation of caprine genetic Kathiravan up to 09.09.11). biodiversity- RAK Aggarwal and D Mallakar (NDRI) - April, 2011 to March, 2014. On-going Research Projects 9. Characterization of Gojri buffalo and 1. Characterization and Evaluation of Sanchori Belahi cattle populations under migration and Nari Cattle of Rajasthan - PK Singh, RK in Foot Hills and Sub-Himalayan regions of Pundir, DK Sadana and HS Rathore (LPPS, Northern India- Vikas Vohra, SK Niranjan Sadri) - April, 2011 to March, 2014. and AK Mishra (w e f. Sept., 2012) -October, 2011 to March, 2014.
2. Characterization and Evaluation of ANNUAL REPORT 2012-2013 Indigenous Cattle Germplasm in Northern 10. Development of breed signature for Sahiwal, Eastern States of India- RK Pundir, PK Gir and Tharparkar cattle- MS Tantia -April, 2012 to March, 2015. 2012-13 47 NBAGR
ANNUAL REPORT 2012-2013 48 RESEARCH PROJECTS 14. 13. 12. 11. NBAGR 16. 15. 17. RESEAR
in mammalian culturecell system. Tissue Plasminogen Activator gene construct Development and validation of human 2012) -April, 2011to March, 2014. Arora, DK Yadav and S Bhatia (up to Feb., mutton quality traits inIndian sheep. -Reena Nucleotide diversity in candidate genes for 2013. October., 2011)-January, 2010to December, M Mukesh and Indrajit Ganguly (we f. indicus of Toll like receptors in Indian native Bos Delineating polymorphism and evolution to December, 2013. and NK Verma.- Behl Rahul January, 2009 indicus Cytokines (Bos Study of genetic the variability Bovine inthe and SK Tomar (NDRI) -19 2012), Rameshwar Singh (up to 03.09.2012) 18.05. 2012)Dinesh Kumar (up to 18.05. identification. -Karan Veer Singh (w.e.f. andfoods sequencing of 16SrDNA for strain of microorganisms from fermented dairy (ongoing, ICAR Funded Project): Isolation Agriculture and Applied (AMAAS) Sectors Application of Microorganisms in 2012 to March, 2014. - Karan Veer Singh and Jayakumar S-April, in Non-Traditional animals (NTDA) dairy of SNPs and association their traits with milk Profiling constituents,of milk identification March, 2015. Ganguly and Sanjeev Singh- April, 2012to Resources- Network Project on Animal Genetic 2014. 31 st March, 2012,Extended up to March, 2012- cattle breeds -Monika , Sodhi BK Joshi, Project coordinator CH PROJECTS &PUBLIATIONS ). - Jyotsna Behl, th July, 2006to - Indrajit External FundedProjectsExternal 23. 22. 21. Identification of Quantitative Trait Loci 20. 19. 18.
2008 to March, 2014 Monika (w.e.f. Sodhi January, 2012) -July CCPI-up to 24.02.11),RSKataria and biomarkers(NAIP)- MMukesh, (BP Mishra for identification of probable mammary involution in indigenous cattle and buffalo and proteome during lactation and Analysis of gland transcriptome mammary - July, 2008to December, 2013 Niranjan and Sanjeev Singh w.e.f. 24.09.2011 (NAIP) -RSKataria, SK disease resistance Evolutionary lineages and application in Toll-like receptors infarm animals- to March, 2013.Extended upto 2013. Sept. DN Shinde and RLBhagat -January, 2008 (NAIP)-Buffaloes. RK Vijh, Gokhale,SB for yield, Milk Fat and Protein Percent in (NDRI) June, -4th 2010to 3rd June, 2013. Mukesh and Rajesh Kumar and AKTyagi traits for (DBT) healthfulness. -SP Dixit, M goats and association their quality with milk Identification of SNP’s in QTL region in Indian April, 2012to March, 2014 and Sanjay Mandakmale (MPKV, - Rahuri) f 1.08.12),Manoranjan Roy (AH&VS WB) Sharma, Ahlawat Sonika (on study leave we precocitysexual inindigenous goats. -Rekha Characterization of candidate genes for 2013 December Ahlawat and Sharma Rekha - goat. of variants allelic of fecundity inindigenous Candidate gene analysis and identification Contd. and MSTantia (I/c,Network Project) -1992-
(Core Lab-Network Project-) -Sonika 13 April, 2011to jk-i-vk-la-C;wjks RESEARCNBAGRH PROJECTS & PUBLICATIONS
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24. Bio-prospecting of Genes and Allele Mining 3. Ahlawat S, Sharma R and Maitra A (2012) for Abiotie Stress Tolerance. (NAIP) - RK Vijh Nucleotide diversity in promoter of bone CCPI and Animal Sciences Group Leader morphogenetic protein receptor type 1B -April, 2009 to March, 2013. Extended upto (BMPR1B) gene in Indian goats. Journal of March, 2014. Biotechnology and Biomaterials, 2(6): 44.
25. Establishment of National Agricultural 4. Ahlawat S, Sharma R and Maitra A (2013).
Bioinformatics Grid (NABG) in ICAR. Screening of indigenous goats for prolificacy O NS PU B LICATI (NAIP) -Avnish Kumar, DK Yadav, Dinesh associated DNA markers of sheep. Gene, Kumar (BT), B Prakash and PK Vij - April, 517:128–131. 2010 to March, 2014. 5. Ahlawat S, Sharma R and Maitra A. (2011) 26. Harmonizing Biodiversity conservation (Published in 2013). Fec B mutation and Agricultural Intensification through responsible for fecundity in sheep not Integration of plant Animal and fish genetic detected in Indian goats- a short note. resources for livelihood security in fragile Journal of Livestock Biodiversity, 3(1):28-30. ecosystem (NAIP) - BK Joshi, Anand Jain, PK Vij, NK Verma, RAK Aggarwal KN Raja 6. Ahlawat S, Sharma R and Maitra A. (2012). and Vikas Vohra (w.e.f 10th July 2012)- Analysis of coding DNA sequence of GDF9 September, 2009 to June, 2013. gene in Indian goats for prolificacy associated markers. Indian Journal of Animal Sciences, 27. Genome data mining to unravel molecular 82(7): 721–725. basis of thermo tolerance and adaptation to diverse environment in native cattle 7. Ahlawat S, Sharma R, Maitra A (2012) and buffaloes. (National Fellow) - Manishi Prolificacy genotypes of Bone Morphogenetic Mukesh - 17th Feb., 2011 to 2016. Protein 15 (BMP15) gene in sheep were not detected in Indian goats. Indian Veterinary Publications Practitioner, 13 (2): 234-240. Research Articles 8. Arora R, Yadav DK and Yadav HS (2013). SNP analysis of Growth differentiation 1. Aggarwal J, Sharma A, Kishore A, Mishra factor 8 (GDF8) gene in Indian sheep. Indian BP, Yadav A, Mohanty A, Sodhi M, Kataria Journal of Animal Science, 83 (3): 304–306. RS, Malakar D and Mukesh M (2012). Identification of suitable housekeeping genes 9. Arora R, Yadav HS and Mishra BP (2013). for normalization of quantitative real-time Mitochondrial DNA diversity in Indian PCR data during different physiological sheep. Livestock Science, 153: 50–55. stages of mammary gland in riverine 10. Banerjee P, Gahlawat SK, Joshi J, Sharma buffaloes (Bubalus bubalis). Journal of U, Tantia MS, Vijh RK (2012). Sequencing, Animal Production and Nutrition, DOI: Characterization and Phylogenetic analysis 10.1111/jpn.12027. of TLR genes of Bubalusbubalis. DHR 2. Ahlawat S and Sharma R (2013). Contribution International Journal of Biomedical and Life of goat to rural poverty alleviation and food Sciences, 3(1): 137-159. ANNUAL REPORT 2012-2013 security. Livestock Line. 6 (11): 11-14 11. Banerjee P, Joshi J, Sharma U and Vijh RK (2012). Population Differentiation in 2012-13 49 NBAGR
ANNUAL REPORT 2012-2013 50 PUBLICATIONS NBAGR 17. 16. 15. 14. 13. 12. RESEAR Research, 40:17-19. riverine buffalo. Journal of Applied Animal A1551G polymorphism inTLR8gene of ARMS-PCR assay for detection the of RS (2012).Development of tetra primers SK, Kathiravan P, Mishra BP and Kataria J, PK, Goyal S,Aggarwal Dubey Gahlawat 45:91-99. Tropical Animal Health and Production, of bubalis Indian riverine buffalo (Bubalus characterization of Toll-like receptor 8gene andRS (2012).Sequence topological SK, Kathiravan P, Mishra BP and Kataria J, PK, Aggarwal Dubey Goyal S,Gahlawat RuminantSmall Research, 105:38-45. goat breeds on microsatellite based markers. Diversity and relationship among Indian Vyas J, MK, Rana Sharma A (2012). Genetic Dixit SP, Verma RAK, NK,Aggarwal Journal of 83(4):423–427. Animal Sciences, variability in Surti breed of goat. Indian management, performance and genetic SB. (2013).Phenotypic characteristics, Kharadi VB, Sabapara GPand Deshpande Verma NK, Vyas J, MK, Rana Sharma A, Dixit SP, Dangi RAK, PS, Aggarwal of Epizootics) 31:919-930. scientifiqueet technique (International Office swine fever pygmy inthe hogs. Revue GSandRS, Desai (2012).Classical DekaPJ DP,Barman NN,Bora Tiwari AK,Kataria 1205–1212. Indian Journal of 82(10): Animal Sciences, humped camel of India (Camelusbactrianus characterisationRK. (2012).Genetic of two P,Banerjee Joshi J, Sharma U, Ganai Nand Vijh and Veterinary Advances, 4(2):84-92. Distribution. International Journal of Animal Camel inhabiting Extremes of Geographical Dromedarian camel: AComparative Study of 2012- CH PROJECTS &PUBLIATIONS ). ). ).
23. 22. 21. 20. 19. 18. 24. ( epithelialmammary cells in riverine buffaloes genes for qRT-PCR analysis of heat-stressed M. Identification of appropriate reference Kumar P, Mohanty AK, Jerath T and Mukesh Kapila N,Kishore M,Sharma A, A,Sodhi 3(1): 93-105. Journal of Biomedical and Life Sciences, of Indo-Gangetic Plains. DHR International evaluation of Murrah breeds with buffaloes Tantia MSand Vijh RK(2012).Comparative Joshi J, P, Salar RK, Banerjee Sharma U, 82(11):1434-1437. Animal Sciences, of Indo-Gangetic Plains. Indian Journal of (2012). Microsatellite analysis of buffaloes Sharma U, Gaur U, Tantia MSand Vijh RK Joshi J, P, Salar RK, Banerjee SB, Gokhale 1259-1275. Indian Journal of 82(11): Animal Sciences, animal genetic resources of India: A review. characterizationBP (2012).Genetic of farm Joshi M,Mukesh BK, Sodhi Mand Mishra Journal of 83(1):46–50. Animal Sciences, binding sites for transcription factors. Indian identity with cattle but variations inpotential analysisSequence amino reveals high acid SK and Jayakumar S(2013).Yak TNFαgene: Goyal S,Kathiravan p, Kataria RS,Niranjan 40: 140-148. International Journal of Immunogenetics, distinct relationship with cattle and sheep. Toll-like receptor (TLR)genes 1-10reveals characterizationSequence of river buffalo BP, Gahlawat SK and Kataria RS(2013). PK, Goyal S,KathiravanDubey P, Mishra microsatellite markers for parentage testing BP (2012). Power of exclusion of 19 Kathiravan P, Kataria RSand Mishra http://dx.doi.org/10.1155/2013/ 735053 Bubalus bubalis Bubalus 13 ). ISRN Biotechnology, jk-i-vk-la-C;wjks RESEARCNBAGRH PROJECTS & PUBLICATIONS
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in river buffalo (Bubalus bubalis). Molecular Indian Journal of Animal Sciences, Biology Reports, 39: 8217-8223. 82(11):1442-1445.
25. Khatri I, Upasna S, Banerjee P, Joshi J and 32. Prakash B, Pundir RK, Singh PK, Sharma D, VijhRK. (2012). Genome wise identification Neelkant and Singh CV (2012). Assessment of long terminal repeats in Bubalus bubalis. of genetic variability in Hill cattle of Garhwal Indian Journal of Animal Sciences, 82(4): region of Uttarakhand by microsatellite
392-396. markers. Indian Journal of Animal Sciences, O NS PU B LICATI 82 (11): 1353–1357. 26. Kishore A, Sodhi M, Khate K, Kapila N, Kumari P, Mukesh M. (2013). Selection 33. Pundir RK, Singh PK, Neelkant, Sharma D, of stable reference genes in heat stressed Singh CV and Prakash B (2013). Uttara- A peripheral blood mononuclear cells of new cattle germplasm from Uttarakhand tropically adapted Indian cattle and buffaloes. hills. Indian Journal of Animal Sciences, 83 Molecular Cellular Probes (doi:pii: S0890- (1): 51-58. 8508 (13) 00013-3. 0.1016/j.mcp.2013.02.003 34. Pundir RK, Singh PK, Sharma D, Saini A, 27. Kishore A, Sodhi M, Sobti RC, Mukesh M, Singh CV and Prakash B (2012). Hill cattle Mishra BP (2012). Variation analysis in the of Pithoragarh district of Uttarakhand. regulatory region of alpha S1-casein milk Indian Journal of Animal Sciences, 82(12): protein gene among tropically adapted Indian 135-137. zebu cattle (Bos indicus). ISRN Biotechnology http://dx.doi.org/10.5402/2013/926025. 35. Raja KN, Jain A, Singh G, Kumar L,Yadav HK and Arora R (2012). Ramnad White Sheep- 28. Mckenzie GW, Arora R and Hickford JG H phenotypic and genetic characterization. (2012). Genetic variation in the 5 UTR of Indian Journal of Animal Sciences, 82 (9): the KRT2.13 gene of sheep. Animal Science 1082–1086. Journal, 83: 194–198. 36. Saste SR, Ghalsasi PM, Kataria RS, Joshi BK, 29. Mishra P, Ali AS, Aggarwal RAK, Dixit Mishra BP and Nimbkar C (2012). ARMS-PCR SP, KawitkarVS, Dangi PS and Verma NK. as an alternative, cost effective method for (2012). Genetic diversity and bottleneck detection of FecB genotype in sheep. Indian analysis of KonkanKanyal goats. Animal Journal of Biotechnology, 11: 274-279. Genetic Resources, 50: 43-48. 37. Sharma A, Gautam B, Jayakumar S, Saroha 30. Mishra P, Ali AS, Dixit SP, Aggarwal V, Verma NK and Dixit SP (2013) Genetic RAK, Dangi PS, Tyagi N, Dash SK and. structuring of nine Indian domestic goat Verma, NK.(2013). Microsatellite based breeds based on SNPs identified in IGF-I genetic evaluation of Ghumusar goats of gene. Animal Biotechnology, 24: 148-157. Orissa, India, Animal Genetic Resources, (doi:10.1017/S2078633612000811) 38. Sharma R, Maitra A and Ahlawat S (2012). New genetic polymorphism of goat KiSS- 31. Mishra P, Ali AS, Verma NK (2012), 1 gene. Journal of Biotechnology and
Phenotypic, Biometric and Genetic Biomaterials, 2 (6): 41 ANNUAL REPORT 2012-2013 Characterization of Bundelkhandi Goats. 39. Sharma R, Maitra A, Pandey AK and Mishra BP (2012). Differentiation of four Indian 2012-13 51 NBAGR
ANNUAL REPORT 2012-2013 52 PUBLICATIONS NBAGR 46. 45. 44. 43. 42. 41. 40. RESEAR DOI: 10.3923. DOI: goat. International Journal of Meat Science, polymorphism of CAPN1 gene in Sirohi Maitra Aand Mishra BP (2012).Genetic Singh LV, Tripathi V, Sharma Pandey R, AK, 21 (2):1-4. breed. Indian Journal of Veterinary Research variation in CAPN1 gene in Sirohi goat V, Maitra Aand Mishra BP (2012).Genetic Singh LV, Sharma Pandey R, AK, Tripathi Biotechnology, 1:38-41. indigenous breed of Cibtech yak. Journal of keratin associated protein (KAP7) gene in and Gupta Analysis N (2012). Sequence of Shukla S,Shukla SK, Kumar S,Gupta SC Sharma A,Chopra M,GuptaV, Kumar K, Singh LV, Jayakumar S, Kaur N, Kumari N, Journal of 82(5):491–494. Animal Sciences, markers inwater buffalo genome. Indian VijhRK (2012).Distribution of satellite Sharma U, P, Banerjee Joshi J, Khatri Iand Veterianary Advances. 4(4):292-302. Internationalseq. Journal of Animal and tissues of Goat (Capra hircus) Using RNA- (2012). Ubiquitous Expression of in Genes Sharma U, P, Banerjee Joshi Jand Vijh RK Veterinary Advances, 4(4):284-291. Plains. International Journal of Animal and inbuffaloes structuring of Indo Gangetic Discontinuities(2012). Genetic and Sub Sharma U, P, Banerjee Joshi Jand Vijh RK Veterinary Advances. 4(4):272-283. 2012. International Journal of Animal and ( (2012). Identification of SNPs inGoats Sharma U, P, Banerjee Joshi Jand Vijh RK 48(6):611–617. (Genetika), STR markers. Russian Journal of Genetics cattleaboriginal populations by revealed Capra hircus Capra 2012- CH PROJECTS &PUBLIATIONS ) using RNA-Seq Analysis. 53. 52. 51. 50. 49. 48. 47. 153-163. Journal of and Biology life4(1): Sciences, bubalis of GPX1and DIO1 inBubalus Genes Tantia MS,VijhRK (2013). Characterization Dureja V, P, Singh S,Banerjee S,Rana Joshi J, Journal of 82(8):875–878. Animal Sciences, (TLR-2 and TLR-4) Indian inbuffaloes. and Sequencing of Toll Receptor Like genes Sharma Uand Vijh RK (2012). Phylogeny Tantia MS, Mishra P, B, Banerjee Joshi J, 44:1587–1592. Tropical Animal Health and Production, bubalis traits inriver (Bubalus buffalo and its association production with milk (2012). PCR-SSCP analysis of leptin gene BP, Niranjan SK, Singh KPand Kataria RS Tanpure T, PK, Kathiravan Dubey P, Mishra 16(5): 856 Journal and of Metabolism, Endocrinology hypothesis.health: A1/A2 milk Indian Joshi BK proteins (2012).Milk and human M,MukeshSodhi M,.Kataria RS,Mishra BP, 82:183-186. Sciences, of gene. β-casein Indian Journal of Animal crossbred breeding bulls for A1/A2variants Joshi BK (2012).Screening of taurine and Prakash B, Kapil Khate R, K,Kataria RSand M,MukeshSodhi M,Mishra BP, Kishore A, 10.1111/j.1439-0388.2012.01004 of Animal Breeding and DOI: Genetics, explantsmammary Journal of buffaloes. for transcriptional studies inheat stressed Evaluating suitable internal control genes Varshney Nand Mukesh M(2012). Mishra BP, Kataria RS,Mohanty AK, M,KishoreSodhi A,Khate K,Kapila N, 82(12):131-134. Animal Sciences cattle of Tamil Nadu. Indian Journal of physical features of migratory Pulikulam Vivekanandan P(2012). Management and Singh PK, Pundir RK,Kumarasamy Pand 13 jk-i-vk-la-C;wjks ). ). . RESEARCNBAGRH PROJECTS & PUBLICATIONS
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54. Varshney N, Mohanty AK, Kumar S, Kaushik 61. Yadav P, Mukesh M, Kataria RS, Yadav JK, Dang AK, Mukesh M, Mishra BP, Kataria A, Mohanty AK, Mishra BP (2012). RS, Kimothi SP, Mukhopadhyay TK, Malakar Semi-quantitative RT-PCR analysis of fat D, Prakash BS, Grover S and Batish, VK (2012). metabolism genes in mammary tissue of Selection of suitable reference genes in milk lactating and non-lactating water buffalo somatic cells of lactating cows (Bos indicus) for (Bubalus bubalis). Tropical Animal Health quantitative gene expression studies. Journal of Production, 44(4):693-696.
Dairy Science, 95(6):2935-45 O NS PU B LICATI 62. Yadav P, Singh DD, Mukesh M, Kataria RS, 55. VermaNK, Aggarwa1RAK, Dixit SP, Yadav A, Mohanty AK, Mishra BP (2012). KawitkarVS, Dangi PS, Kaur N, Mishra Identification of suitable housekeeping P and Joshi BK (2012). Konkan Kanyal – genes for expression analysis in mammary Characters and performance of a newly epithelial cells of buffalo (Bubalus bubalis) discovered goat germplasm of Maharashtra during lactation cycle. Livestock Science, state. Indian Journal of Animal Sciences, 147 (1):72-76. 82(9):1079-1081. Popular/Technical Articles 56. Vohra V, Niranjan SK and Joshi BK (2012). Belahi Cattle: Uniform but distinct 1- vkj ,l dVkfj;k] ch ih feJk] ch izdk'k germplasm of Haryana. Journal of Animal ,oa Mh ds lnkuk (2012) iwoksZÙkj Hkkjr Research, 2(1): 47-51 dh foy{k.k HkSal uLysaA i'kq/ku izdk'k 3% 57. Vohra V, Niranjan SK and Joshi BK (2012). 11&14- Gojri: A novel migratory buffalo germplasm in Punjab and Himachal Pradesh. Journal of 2- izrki flag i¡okj ,oa fodkl oksgjk (2012) Animal Research, 2(3):317-321. Hkkjrh; HkSalA i'kq/ku izdk'k 3% 74&75-
58. Yadav DK, Jain A, Kulkarni VS, Govindaiah 3- fodkl oksgjk ,oa izrki flag i¡okj (2012) MG, Aswathnarayan T, Sadana DK i'kq vkuqoaf'kd lalk/ku dk lr~r mi;ksxA (2013). Classification of four ovine breeds i'kq/ku izdk'k 3% 71&73- of southern peninsular zone of India: Morphometric study using classical 4- bUæthr xk¡xqyh ,oa latho flag (2012) discriminant function analysis. SpringerPlus ÅaV nw/k dk iks"k.k vkSj fpfdRlh; igywA i'kq/ 2:29. doi:10.1186/2193-1801-2-29 ku izdk'k 3% 84&88- 59. Yadav DK, Vij PK. Cattle herd structure 5- fot ih-ds-] vxzoky vkj- ,-ds-] VkafV;k and herd dynamics in Gaushalas of Haryana. Journal of Livestock Biodiversity eèkqlwnu] 'kekZ iadt ,oa tks'kh ch-ds-] 3 (1): 35-38. (2012) fgekpy izns'k ds pack fT+kys esa i'kqèku fofoèkrk] i'kq/ku izdk'k 3% 5&10- 60. Yadav HS, Gupta R, Mishra BP and Arora R (2012). Assessment of genetic relationships 6- euh"kh eqds'k] vfer fd'kksj] izos'k eqatky] between Kheri sheep and its founder breeds eksfudk lks<+h (2012) cnyrs oSf'od ifjos'k using molecular coancestry information. esa Hkkjrh; i'kq/ku lEink & ,d voyksduA ANNUAL REPORT 2012-2013 Indian Journal of Dairy Science. 65(6):484- i'kq/ku izdk'k 3% 64&70- 489. 2012-13 53 NBAGR
ANNUAL REPORT 2012-2013 54 PUBLICATIONS - js[kk 7- NBAGR 15. 14. 13. - lksfudk 9- lksfudk 8- 11- 10- iqaMhj 12- ch-ds- RESEAR
Quality. Livestock International, 16(1): 14-15. Molecular markers inAssessment of Meat Sharma Ahlawat R, Sand Maitra A.(2012). Livestock International,16(3): 19-21. ruminants:in small far. The so journey Sharma R and Ahlawat S.2012.Selection July 2012,pp 30-33. ResourcesGenetic of India. Indian Farming, Priyanka Mand Verma NK(2012). Goat xk;ksa vkSj esa vkSj dyAi'kq/kuizdk'k3%34&36- y?kq 79&83- Ïf"k Hkkjrh; (vxLr) 19&21- mRrjk[kaM izdkf'kr) (2012) ÞlqjfHkÞ vuqlaèkku 13&19- i`"B 17&19- Þe# dk mUur ih-ds- fFkysfj;ksfll 2012- vkuqoaf'kd #eaFkh Hkfo"; esa 'kekZ Ïf"k fdj.k vkj-ds- iztuu flag vgykor xHkZ tks'kh izeq[k ds vgykor ih-Mh-lh- ,oa p;fudk laLFkku] jtr i'kqvksa ijh{k.k dh dh CH PROJECTS &PUBLIATIONS vkSj xks 4% vkSj lksfudk rduhdksa laHkkouk,aA fodklA jksx ,oa vkSj uLysa% xk; t;arh esa d#.kk ,oa 96&98- js[kk dh p;u (i'kq ds tks/kiqj esjB ih-ds- 'kekZ igpku dk fof/k;k¡ js[kk vgykor 'kekZ o"kZ dkj.k dsUæh; }kjk vfltk ikyu izfØ;k% i'kq/ku dh esa ewY;kadu] (2011)- 'kekZ flag Mh- }kjk ,oa izdkf'krA orZeku vkSj ikyrw fgUnh (2012 ,fjM fo'ks"kkad)ÞA mi;ksfxrkA (2012)] (2012)- (2012)- izdk'k dy (2012) (2012) fuokj.kA izdkf'kr if=dk i'kqvksa fLFkfr i'kqvksa vkt [ksrh tksu i`"B 3% esa
16. 2. 1. Monographs/Bulletin/Leaflet Breed of Bihar. Livestock International. 16(4):2-4 Shahabadi: An important cattle germplasm Sharma Singh PK andR, Ahlawat S (2012). Kataria and BK Joshi. Monograph #72,2012. R Venkataramanan, MIyue, BP Mishra, RS Nadu by PKathiravan, SMK Karthickeyan, Toda- The Unique of Hill Buffalo Tamil Monograph #68,2012. DK Sadana PKathiravan and BK Joshi. India by BP Mishra, KPSingh, RSKataria, Banni- AUnique Germplasm of Western 13 jk-i-vk-la-C;wjks RESEARCNBAGRH PROJECTS & PUBLICATIONS
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3. Buffalo Genetic Resources of India- Marathwadi by RS Kataria, P Kathiravan, BP Mishra, DK Sadana, BV Gujar, V Vohra and BK Joshi. Monograph # 73, 2012.
4. Berari-An important goat germplasm of Vidarbha by NK Verma, SV Kuralkar, RAK Aggarwal, SP Dixit, Priyanka Mishra, Prajakta Kuralkar, PS Dangi, and BK Joshi. Monograph# 76, 2012 Dr. R.S. Kataria, Principal Scientist, receiving Dr. P.G. Nair award (2012) from Dr. B. Pisupati, Chairman, National Biodiversity Authority 5. Sheep Genetic Resources of India, Narayanpatna goats of Odisha” by PS Dangi,
Madras Red by A K Mishra, K N Raja, D. O GNITI NS AWARDS/REC NK Verma, SP Dixit, RAK Aggarwal, SK Balasubramanium A Jain, G Singh, P H R Dash and Manoj Sarangi during National Rao, S Singh, V Vohra, S K Niranjan and B Seminar on “New paradigms in livestock K Joshi. Monograph # 72, 2012 production: from traditional to commercial farming and beyond” held at NDRI, Karnal 6. Protocol on “Isolation of mammary epithelial on 28-30 January, 2013. cells (MECs) from milk of cattle and buffalo” by AK Mohanty, Jagdeesh Janjanam, Manu yy Best poster Award for the poster entitled Jamwal, Surender Singh, Vijay Anand J, "Bharat ke pashu aanuvanshik Sansadhan" by Karuna Asija and P K Singh at the occassion Sudarshan Kumar, Neha Charavarty, Pradeep of Foundation day of NBAGR. Behare, Ajay K. Dang, Jai K. Kaushik, D Malakar (NDRI, Karnal), Manishi Mukesh, yy Young Scientist Award for the paper Monika Sodhi entitled “Detection of polymorphism in the nucleotide-binding oligomerization domain 7- Hkkjrh; xks&/ku% ,2 nw/k ds fy, ,d izkÏfrd like receptor-2 gene of Indian riverine lalk/ku by ,e lks ANNUAL REPORT 2012-2013 56 AWARDS/RECOGNITIONS • NBAGR • • RESEAR Kurukshetra, Kurukshetra University. Technology” November 23-24, 2012 at UIET, AmalgamatingBiotechnology: & Science at National the conference on “NexGen in exotic goat using PCR-RFLP method” polymorphism associated with twinning izdk'kÞ vFkZO;oLFkk ys[k MkW eqatky eksfudk x;kA chVk&dslhu 2011 [kkrs vfer euh"kh f}rh; iqjLdkjfn;kx;kA 'kks/k&if=dk ,u ßHkkjrh; 2012- ds- ,oa eqds'k] ,oa fd'kksj] ds oekZ esa eksfudk izFke izdkf'kr lks<+h] ch esa }kjk ,2 ßi'kq/ku vfer CH PROJECTS &PUBLIATIONS cdjh&izca/ku] ;ksxnkuÞ ds Best worker award worker Best being conferred during Foundation Day celebrations okf"kZd ,yhy vkj vad lks y • • y worker awards. Satbir bestthe received (Supporting Staff) officer), Sh.Mahavir (Technicals) and Sh (Administrative), Sh Moti (Technical Ram foundation day of NBAGR. Sh Karambir were conferred on occasion of the workerBest awards for differentcategories izkS|ksfxdh fn;k x;kA 2011 fgUnh MkW pqukSfr;k¡** dksf}rh;iqjLdkjfn;kx;kA ßHkkjrh; izdk'kÞ LFkkiuk MkW egRorkß dsfy,f}rh;iqjLdkjfn;kx;kA ßcnyrs ch lksfudk ds 'kks/k&if+=dk tks'kh ds fnol esa tyok;q i'kq ds izFke izdkf'kr vgykor }kjk {ks= 13 vkuqoaf'kd ij vad okf"kZd esa ifjos'k ßi'kq/ku vk;ksftr ys[k ,d ,oa o"kZ fgUnh Økafr** MkW 2010 lalk/ku ^^lsDlM esa izdk'kÞ js[kk 'kks/k&if=dk iksLVj Hkkjrh; dks 'kekZ esa ds dh r`rh; oh;Z izdkf'kr jk-i-vk-la-C;wjks izfr;ksfxrk f}rh; }kjk i'kq/ku fLFkfr & iqjLdkj ßi'kq/ku iztuu okf"kZd v ys[k ,oa dh ad esa NBAGR jk-i-vk-la-C;wjks NBAGR Other Activities • Library Services • Important Meetings • Institute Technology Management Unit • Trainings / Workshops attended and organized • Celebrations • Sports Activities • Fairs/Exhibitions • Distinguished Visitors • Teaching ANNUAL REPORT 2012-2013 2012-13 57 ANNUAL REPORT 2012-2013 58 NBAGROTHER ACTIVITIES jk-i-vk-la-C;wjks NBAGR Library Services The NBAGR library plays an important role in serving the needs of scientists and technical staff of the Bureau. There is always an intension to equip the library with most suitable books for the researchers. In this regard, library Advisory Committee (LAC) is a guiding force in the management of the library issues specially pertaining to purchase of scientific books/ journals etc. The Bureau LAC was reconstituted Network Project review meeting O THER ACTIVITIES on 15.11.2012. During the year 2012-13, books under the chairmanship of Dr. K.M.L. Pathak, and journals worth Rs. 8,30,846/- were procured DDG (AS), ICAR, Delhi. The meeting was also in the library. Fourteen foreign journals and attended by Dr. S.C. Gupta, ADG (AP&B), thirty four Indian Journals have been subscribed Dr. Gaya Prasad, ADG (AH) and Dr. Vineet for the benefit of scientific readers. Bhasin, Pr. Scientist, Animal Science Division, ICAR, New Delhi. The In-charges of core labs Library Collections under Network project and of buffalo genomics 1. Total collection 3865 presented the reports of their respective units. 2. No. of books added 52 Updated version of cattle and buffalo breed calenders; breed monographs on Toda buffalo 3. No. of Indian Journals subscribed 34 and Berari goat were released on this occasion. 4. No. of Foreign journals subscribed 14 All the scientists from the Bureau also attended 5. No. of News papers subscribed 07 the meeting. Important Meetings Quinquennial Review Team Institute Research Committee (IRC) The Quinquennial Review Team (QRT), headed by Dr. P. Thangaraju, Former Vice-Chancellor, Annual Institute Research Committee meeting for the year 2011-12 was held on 20th, 21st & TANUVAS, Chennai visited the Bureau on 26th 24th April, 2012 under the chairmanship of October, 2012 and 19-20th December, 2012 to Dr. B.K. Joshi, Director, NBAGR. Final reports review the work done by the NBAGR, Karnal, of nine projects completed by March 2012 for the period 2007 to 2012. The In-charges and proposals of eight new research projects of Divisions/Sections made a presentation of were discussed and approved. Mid-term IRC various activities before the QRT. The team meeting was held on 25th & 26th September, visited the laboratories and had interactions with 2012. Progress reports of all the on-going research projects were discussed. The annual the scientists. IRC for the year 2012-13 was held on 28th March 2013. Where in the final reports of four completed projects and five new projects were approved. Annual Review Meeting of Network Project ANNUAL REPORT 2012-2013 11th Annual Review Meeting of Network Project on AnGR was held on 25th August, 2012 Chairman QRT showing interest in lab activities 2012-13 59 NBAGR ANNUAL REPORT 2012-2013 60 OTHER ACTIVITIES Constitution ofN Constitution NBAGR OTHER ACTIVITIES year; Following three patents were the to filed Indian PatentDelhi Office, the by Bureauduring last Patents Institute Technology Unit Management A meeting of NABMGR was held on 5 Management Resources of Genetic National on Board Advisory this occasion.this Sheep and NBAGR Web were portal released on on Sindhi donkey, cattle Purnia and Madras Red DARE News ICAR. Letter, &DG, Monographs and co-chaired by Dr. S. Ayyappan, Secretary 2013. The wasmeeting chaired by Dr.R.S. Paroda 3 2 1 No. S. 7 6 5 4 3 2 1 607/DEL/2013 298/DEL/2013 50/DEL/2013 Application No. Principal Scientist, NBAGR Dr. Pundir, R.K. Member Secretary CSKHPKV, Palampur Ex-Professor &Head, AG&B Deptt. Dr. Kamlesh Gupta, Member NC,Ex- NATP Dr. K.P. Member Agarwal, Ex-Director, CSWRI,Awikanagar Dr. V. K.Singh, Member Dy. Director, CCMB, Hyderabad Dr. K.Thangaraj, Member Nammakkal Professor,Ex- Veterinary College, Dr. N.Kandasamy, Member ViceEx- Chancellor ,TANUVAS, Chennai Dr. P. Thangaraju,Chairman 2012- bagr buffalo meat and milk differentiation cattleof and DNAPCR based test for the in Indian ruminant livestock A kit for parentage verification in goats A kit for parentage verification Patent Title Q rt th March, regarding biosafety issues. awareness among laboratory staff of NBAGR, NBAGR on 30-07-2012,as ameasure to create delivered by Dr. Kataria, R.S. Principal Scientist, granted. Alecture on biosafety guidelines was IBSC were permissions and reviewed necessary research of projects under purview falling the meetings on 06.08.2012and 24.01.2013.The involving biosafety issues, held its six monthly cloning/recombinant DNA work/transgenics researchthe projects of Bureau the engaged in IBSC of NBAGR, constituted for monitoring (IBSC) Institutional Biosafety Committee and Dr. Monika Sodhi Dubey, Dr. S.K.Niranjan Dr. Kataria, R.S. P.K. Upasna Sharma JyotiBanerjee, Joshi and Dr. Vijh, R.K. Priyanka Sharma Joshi, Shivani Upasna Rana, Priyanka Jyoti Banerjee, Dr. Kumar Ramesh Vijh, Inventors NABMGR me 13 eting in progress 4-Mar-13 4-Feb-13 8-Jan-13 patents Date of filing jk-i-vk-la-C;wjks NBAGROTHER ACTIVITIES jk-i-vk-la-C;wjks NBAGR Commercialization of Technology yy Dr. R.K. Vijh, Dr. M.S. Tantia and Dr. RAK Aggarwal attended a training program" Three technologies viz., A kit for parentage Management Development Programme verification in Zebu cattle (Bos indicus), A kit on Leadership Development- (A pre-RMP for parentage verification in Camels (Camelus Programme)" organised at National Academy dromedarius and Camelus bactrianus) and A kit of Agricultural Research Management, for parentage verification in buffaloes (Bubalus Hyderabad from 8th October to 19th bubalis), were commercialized on 5th February October, 2012. 2013. The amounts for which the technologies have been commercialized were Rs. 2.00 Lakhs, yy A summer short course was organised on O THER ACTIVITIES Rs. 1.00 Lakh and 3.00 Lakhs respectively with Genomic and Phenomic tools for the analysis royalty of 1%. The 50% of the cost of technology of livestock genome during June 14- 23, 2012. has been received at the time of signing of The course was sponsored by Education MoA between M/s Sandor Proteomics Pvt. Ltd. Division, Indian Council of Agricultural Hyderabad and NBAGR, Karnal and balance Research (ICAR). 50% shall be paid after grant of patent or 5 years period whichever is earlier for which bank yy A training programme on "Conservation and guarantee shall be obtained by the institute. sustainable utilization of farm animal genetic resources" commenced from 29th January, 2013 and concluded on 2nd February, 2013. The officers from State Animal Husbandry Department, Jammu & Kashmir participated in this training programme. MoA being signed between M/s Sandor Proteomics Pvt. Ltd. Hyderabad and NBAGR, Karnal. Trainings/Workshops attended and organized Dr. Gurubachan Singh, Chairman, ASRB, Delhi yy Dr. R.K. Vijh, Principal Scientist was releasing the training manual deputed for three week training under the NAIP subproject "Bioprospecting of genes and allele mining for abiotic stress tolerance" . The training program entitled " Prediction of genetic merit from genotype data- the analytical system", was organized by Professor James Reecy, Department of ANNUAL REPORT 2012-2013 Animal Science, IOWA State University of Science and Technology, Ames, IOWA, USA from April 27, 2012 to May 17, 2012. Participants and faculty of training programme 2012-13 61 NBAGR ANNUAL REPORT 2012-2013 62 OTHER ACTIVITIES NBAGR OTHER ACTIVITIES day on 21 The Bureau celebrated its Foundation 29th Foundation Day Celebrations y y Chairman, National Biodiversity Authority. was presidedfunction by Dr. B. Pisupati, A Monograph on buffalo, Banni NBAGR sustainable approach for conservation of AnGR. Dr. Pisupati emphasizing holistic the and Foundation Day address was delivered by exhibition was held to mark occasion. the occasion the as Guestgraced of Honor. Aposter Dr. D.K. Sharma, Director, CSSRI, Karnal y y scholars attended the workshop. attended the scholars scientists, administrative staff and research Karnal. Atotal of 43staff members including Izatnagar and by Scientists of ZTM-BPD Unit of IVRI, different of IPR aspects were delivered Karnal. In workshop this four lectures on issues” One day Workshop on “Awareness of IPR Karnal. during February 18-28,2013at NBAGR, Bioinformatics GRID (NABG) in ICAR” “Establishment of National Agricultural was organised under NAIP subproject Improvement of Resources Animal Genetic ‘Bioinformatics for and Conservation Organized ASubject training on Dr. B.Pisupati, releasing the Hindi Magazine 2012- was held on 11.1.2013at NBAGR, st Pashudhan Prakash September, 2012.The In-charge ITMU, NDRI, Republic Day on 26 Independence Day on August, 15th 2012and The NBAGR staff and celebratedfamilies 65th DayIndependence and Republic Day commemorate Vigilance the Day. against corruption on October, 29th 2012 to NBAGR members staff took oath the Vigilance Day family members on occasions. Children these hoisted tricolor the and addressed staff the and campus. Dr. B. K.Joshi, Director, NBAGR this occasion.this liters day. per Thewinners weregiven prizes on production recorded Cattle was 8.4 inBelahi Animal Husbandry Department. Highest milk organized incollaboration with Haryana State and Panchkula of districts Haryana were of villages in the Ambala, Yamuna Nagar Gujjar communities cattle rearing Belahi Milking cow competitions among pastoral the Nagar, Shazadpur block, Ambala (Haryana). Exhibition” on 22nd May 2012 in village Ajeet the “Pashu Mela Resource Animal Genetic cum migratory pastoralist, bureau the organized Day, 2012and to bring awareness amongst To commemorate International Biodiversity International Biodiversity Day by honorable guests. latest issue of "Pashudhan Prakash" were released Paltu Pashu Jaiv Vividhita” (inHindi) and the Newsletter (Jan- June 2012)and CDon “Bhartiya Director NBAGR, addressing the farmers on the occasion of Biodiversity Day 13 th January, 2013inthe jk-i-vk-la-C;wjks NBAGROTHER ACTIVITIES jk-i-vk-la-C;wjks NBAGR held at National Dairy Research Institute, Karnal from April 25-28, 2012. yy In order to commemorate the Institute Foundation Day, Institute Sports Meet-2012 was organized for the Bureau staff and family members from 15-18 Sept., 2012. During the event, various sporting events like basketball, table tennis, badminton, carom, athletics etc. were conducted. The event has attracted an overwhelming response from O THER ACTIVITIES Independence Day celebrations in the campus the participants of all age groups. presented cultural programmes and participated yy The institute volleyball smashing team being in athletic events. the winner during North Zone sports meet, represented NBAGR in the inter-zonal ICAR Breed Saviour Award Ceremony sports meet held at IARI, New Delhi from The function was held on 8th March 2013 at 18-21 January, 2013. NBAGR. Twenty farmers belonging to different yy A 32 member sports contingent represented states were honoured for their contributions in NBAGR to participate in ICAR Inter- breed conservation of indigenous germplasm. institutional sports meet (North Zone) held at IISR, Lucknow, from March 19-22, 2013. Sports Activities The institute team won volleyball smashing yy The institute volleyball team has won and finished runners up in volleyball volleyball smashing and finished runners up shooting events. Sh. Yoginder, bagged third in volleyball shooting events during ICAR position in 400 meter race Assistant. Inter-institutional sports meet (North Zone) Fairs/ Exhibitions yy NBAGR participated in Fish Festival on 22.09.2012 at Rohtak Centre (Lahli) of CIFE. yy NBAGR exhibited its activities in International Conference of the Parties (COP11) to Convention on Biological Diversity (CBD) held at HITEX City, Hyderabad during 2-19 Oct, 2012. ANNUAL REPORT 2012-2013 Participation in Zonal Sports Meet and Winning Sh. Bhupinder Singh Hooda, Hon’ble Chief Minister Contingent with trophies Haryana appreciating the NBAGR publications 2012-13 63 NBAGR ANNUAL REPORT 2012-2013 64 OTHER ACTIVITIES NBAGR OTHER ACTIVITIES Distinguished Visitors y y y y y y y y y y y y y y y y y y y y y y y y Bhubaneswar visited on 05.11.2012. & Technology Department, Govt. of Odisha, Director (Technical), Biotechnology, Science Biotechnology Deputy and Dr. C.C.Rath, Prof. U.B. Mohapatra, Director (Technology), on 13.09.2012. Department, Hansraj visited College, Delhi Faculty and students from Zoology 28.08.2012. Sahjeevan, Kutch visited Bureau the on Dr. Sabyasachi Executive Chief Das, Officer, (AH) visited on 25.08.2012. (AP&B)and Dr.ADG Gaya Prasad, ADG Dr. K.M.L.Pathak, (AS), Dr. DDG S.C.Gupta, on 13.08.2012. Dr. Acharya, Former R.M. (AS) visited DDG 07.07.2012. Dr. Director, Sethi, R.K. CIRBvisited on on 30.05.2012. Dr.Venkateswarlu NBAGR Dasyam visited A delegation from FAO by led from Branala visited Bureau on 29.05.2012. A team of 12farmers by led Sh. Balraj Singh 14.05.2012 for institutional visit. Agriculture and Forests, Bhutan visited on from Royal Govt. of Bhutan, of Ministry A delegation comprising of 7senior officers 16.04.2012. NBAGR onCentre, Bangladesh visited (Livestock), SAARCOfficer Agriculture Dr. (Md.) Nure Alam Siddiky, Program National Livestock Championship 2013. from to Jan 8th 12th 2013on occasion of Gobind Singh Stadium at Muktsar, Punjab An exhibition at was installed Guru showcased exhibition. inthe activities the Divas on 23.12.2012at NDRI, Karnal and in agriculture” on 22.12.2012and Kisan on “Prosperity through diversification NBAGR participated inNational Seminar 2012- for thesis research their work. registered with NDRI Karnal are guided being Ph.D programmes. four students M.Sc. Besides, teachingin the of regular courses of Master and and Biochemistry. They are actively involved Biotechnology, &Breeding Animal Genetics Universities, disciplines inthe of Animal Indian Veterinary Research Institute Deemed ofCouncil National Research Institute Dairy and as Post Graduate by faculity Academic the ScientistsThe of NBAGR have been approved Teaching y y y y y y y y y y y y y y 12.03.2013. Programme, New visited Delhi on South Asia Pro Poor Livestock Policy Dr. Tinni Sawhney, Programme Director, visitedBoard on 06.03.2013. Officer from Punjab LivestockDevelopment The Technical Advisor and Veterinary Bureau on 05.03.2013. other Members of NABMGR the visited the of Agriculture,Ministry GOI, New and Delhi Varieties and Farmers' Rights Authority, Former Chairperson, Protection of Plant Former Chairman, ASRB Dr. P.L. Gautam, Dr. ICAR, M. Mahadevappa, (AS), DDG S.Ayyappan, Dr. ICAR, DG, K.M.L.Pathak, Dr. Paroda, R.S. Former Dr. ICAR, DG, visited NBAGR on 01.02.2013. A 12-member delegation from Bhutan 18.01.2013. ITD) ITC Limited, Bangalore visited on Dr. Parag Vilas Naik, Scientist (IAD&R- Lead looked NBAGR on 22.12.2012. Dr. Singh, Director, R.K. NRCE, Hisar visited Dr. Director, Sethi, R.K. CIRB, Hisar and Dr. K.M.L.Pathak, ICAR, DDG(AS), vis Prof. James from ISU, M.Reecy Ames, USA ited NBAGR on 21.12.2012. 13 jk-i-vk-la-C;wjks NBAGR jk-i-vk-la-C;wjks NBAGR Personnel • Scientific • Technical • Administrative • Skilled Supporting Staff • Appointments/Promotions • Transfers/Joinings ANNUAL REPORT 2012-2013 2012-13 65 ANNUAL REPORT 2012-2013 66 NBAGRPERSONNEL jk-i-vk-la-C;wjks NBAGR Scientific Sr. No. Name of Scientist Designation 1. Dr. B.K. Joshi Director 2. Dr. D.K. Sadana Pr. Scientist 3. Dr. B. Parkash Pr. Scientist 4. Dr. R.K. Vijh Pr. Scientist PERS O NNEL 5. Dr. Anand Jain Pr. Scientist 6. Dr. M.S. Tantia Pr. Scientist 7. Dr. N.K.Verma Pr. Scientist 8. Dr. P.K. Vij Pr. Scientist 9. Dr. R.A.K. Aggarwal Pr. Scientist 10. Dr. P.K. Singh Pr. Scientist 11. Dr. R.K. Pundir Pr. Scientist 12. Dr. R.S. Kataria Pr. Scientist 13. Dr. Anil Kumar Mishra Pr. Scientist 14. Dr. Monika Sodhi Pr. Scientist 15. Dr. Jyostna Behl Pr. Scientist 16. Dr. Satpal Dixit Pr. Scientist 17. Dr. Dinesh Kr. Yadav Pr. Scientist 18. Dr. Manishi Mukesh National Fellow 19. Dr. Reena Arora Pr. Scientist 20. Dr. Rahul Behl Sr. Scientist 21. Dr. Avnish Kumar Sr. Scientist 22. Dr. Dinesh Kumar Sr. Scientist (upto 18-05-2012) 23. Dr. Rekha Sharma Sr. Scientist 24. Dr. Vikas Vohra Sr. Scientist 25. Dr. Saket Kr. Niranjan Sr. Scientist 26. Dr. Indrajit Ganguly Sr. Scientist 27. Dr. P. Kathiravan Sr. Scientist 28. Dr. Sanjeev Singh Sr. Scientist 29. Dr. Karan Veer Singh Scientist (S.S) 30. Dr. K.N. Raja Scientist 31. Dr. Jayakumar S. Scientist 32. Dr. Sonika Ahlawat Scientist Technical S. No. Name Designation 1. Dr. P. S. Dangi T (7/8) 2. Sh. S. K. Jain T (7/8) 3. Dr. P.S. Panwar T-6 4. Sh. Sanjeev Mathur T-6 5. Sh. Moti Ram T-5 ANNUAL REPORT 2012-2013 6. Sh. Harvinder Singh T-5 7. Sh. Sat Pal T-5 8. Sh. Jamer Singh T-5 2012-13 67 NBAGR ANNUAL REPORT 2012-2013 68 PERSONNEL Skilled Supporting staff Skilled Supporting Administrative NBAGR PERSONNEL 5. 4. 3. 2. 1. S. No. 19. 18. 17. 16. 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. S. No. 18. 17. 16. 15. 14. 13. 12. 11. 10. 9. S. No. 2012- Sh. Chand Ramesh Sh. OmPrakash Sh. Vijay Singh Sh. Mahavir Singh Sh. Ashok Kumar Sh. Naresh Kumar KumarSh. Rakesh Sh. Subhash Chander Sh. Kumar Ramesh Smt. Pravesh Kumari Name Sh. Babu Ram Sh. Naresh Kumar Smt. Neerja Kaul Sh. Rajnish Kumar Sh. Shiv Chander Sh. Satish Kumar Sh. Sopal Sh. Yoginder Smt. Shashi Bala Sh. Jita Ram Smt. Indu Steno Bala, Smt. Amita Kumari Smt. Anita Chanda Sh. Behl Ramesh Sh. Pawan Kr. Gupta Sh. Karambir SinghSh. Balkar Sh. Sunil Kumar Sh. Jagtar Singh Name Sh. Satbir Sh. Deepak SagarSh.Ram Ram Sh. Sewa Sh. Krishan Lal Name LDC LDC LDC LDC LDC UDC UDC Assistant Assistant Assistant Steno Gr.III PA PA Assistant AF&AO PS to Director AAO F&AO A.O. Designation T-3 T-3 T-4 T-4 T-4 T-4 T-4 T-4 T-4 T-5 Designation 13 SSS SSS SSS SSS SSS Designation jk-i-vk-la-C;wjks NBAGRPERSONNEL jk-i-vk-la-C;wjks NBAGR Appointments/Promotions 8. Sh. Pawan Kumar Gupta has been promoted from JAO to the post of AF&AO w.e.f. 1. Dr. R.S. Kataria, Sr. Scientist has been 29.12.2012 (AN). promoted to the post of Principal Scientist w.e.f. 01.01.2009. 9. Sh. Balkar Singh has been promoted to the post of Asstt. Admn. Officer w.e.f. 2. Dr. Monika Sodhi, Sr. Scientist has been PERS O NNEL 15.06.2011. promoted to the post of Principal Scientist w.e.f. 28.09.2010. 10. Granted 2nd financial upgradation to Sh. Karambir, PS to Director w.e.f. 3. Dr. Jyotsna Behl, Sr. Scientist has been 03.03.2013. promoted to the post of Principal Scientist w.e.f. 29.09.2010. Transfer/joining: 1. Sh. Jagtar Singh joined NBAGR, Karnal 4. Dr. Dinesh Kumar Yadav, Sr. Scientist has as Admn. Officer on 14.05.2012 on inter- been promoted to the post of Principal institutional transfer from ICAR Research Scientist w.e.f. 05.05.2011. Complex, Goa. 5. Dr. Reena Arora, Sr. Scientist has been 2. Dr. Anil Kumar Mishra was selected as promoted to the post of Principal Scientist Principal Scientist and joined NBAGR on w.e.f. 25.07.2011. 29.08.2012. He was promoted as Principal Scientist w.e.f. 01.01.2009 through Career 6. Smt. Parvesh Kumari, T-4 has been Advancement Scheme. promoted to the next higher grade of T-5 through five yearly assessment w.e.f. 3. Dr. Dinesh Kumar, Sr. Scientist 01.07.2012. (Biotechnology) was relieved from NBAGR, Karnal to join CABIN, IASRI, New Delhi on 7. Sh. Sunil Kumar has been promoted 18.05.2012. from AF&AO to the post of F&AO w.e.f. 18.12.2012 (AN). 4. Sh. Yoginder joined at NBAGR as Assistant w.e.f. 29.06.2012. 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