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ULBP2 ELISA Kit Human (OKBB01116) Instructions For

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ULBP2 ELISA Kit Human (OKBB01116) Instructions For ULBP2 ELISA Kit Human (OKBB01116) Instructions for Use cell culture supernates, cell lysates, serum and plasma (heparin, EDTA) Variation between lots can occur. Refer to the manual provided with the kit. This product is intended for research use only. Table of Contents 1. Background ............................................................................................................................................. 2 2. Assay Summary ..................................................................................................................................... 3 3. Storage and Stability .............................................................................................................................. 3 4. Kit Components ...................................................................................................................................... 3 5. Precautions ............................................................................................................................................. 4 6. Required Materials Not Supplied ......................................................................................................... 4 7. Technical Application Tips .................................................................................................................... 4 8. Reagent Preparation .............................................................................................................................. 5 9. Sample Preparation Guidelines ........................................................................................................... 6 10. Assay Procedure .................................................................................................................................... 7 11. Calculation of Results ............................................................................................................................ 8 12. Typical Expected Data .......................................................................................................................... 9 13. Technical Resources ...........................................................................................................................11 www.avivasysbio.com – 888.880.0001 – [email protected] 1 1. Background • Principle Aviva's human ULBP2 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ULBP2 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G26-S216) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ULBP2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ULBP2 amount of sample captured in plate. • Target Background UL16 binding protein 2 (ULBP2) is a cell surface glycoprotein encoded by ULBP2 gene located on the chromosome 6. This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. • General Specifications General Specifications Range 62.5pg/ml-4000pg/ml LOD <12pg/ml (Derived by linear regression of OD450 of the Mean Blank + 2xSD) Human ULBP2 UniProt: Q9BZM5 Specificity Gene ID: 80328 Target Alias: N2DL2, RAET1H, RAET1L, NKG2DL2, ALCAN-alpha Cross-Reactivity No detectable cross-reactivity with other relevant proteins www.avivasysbio.com – 888.880.0001 – [email protected] 2 2. Assay Summary 3. Storage and Stability • Upon receipt store kit at -20°C until expiration date. Avoid multiple freeze/thaw cycles. 4. Kit Components • The following reagents are the provided contents of the kit. www.avivasysbio.com – 888.880.0001 – [email protected] 3 Storage Description Quantity Conditions 96-Well Plate Pre-coated with Anti-ULBP2 Antibody 96 Wells (12 x 8 Well Strips) Lyophilized Recombinant ULBP2 Standard 2 x 10 ng 100X Biotinylated Anti-ULBP2 Antibody 1 x 130 μL 100X Avidin-Biotin Peroxidase Complex (ABC) 1 x 130 μL Store at - Sample Diluent Buffer 1 x 30 mL 20°C until expiration Antibody Diluent Buffer 1 x 12 mL date ABC Diluent Buffer 1 x 12 mL TMB Color Developing Agent 1 x 10 mL TMB Stop Solution 1 x 10 mL 10X Wash Buffer 1 x 30 mL 5. Precautions • Read instructions fully prior to beginning use of the assay kit. • Any deviations or modifications from the described method or use of other reagents could result in a reduction of performance. • Reduce exposure to potentially harmful substances by wearing personal protective lab equipment including lab coats, gloves and glasses. • For information on hazardous substances included in the kit please refer to the Material Safety Data Sheet (MSDS). • Kit cannot be used beyond the expiration date on the label. 6. Required Materials Not Supplied • Microplate reader capable of reading absorbance at 450 nm. • Automated plate washer (optional). • Pipettes capable of precisely dispensing 0.5 µL through 1 mL volumes of aqueous solutions. • Pipettes or volumetric glassware capable of precisely measuring 1 mL through 100 mL of aqueous solutions. • New, clean tubes and/or micro-centrifuge tubes for the preparation of standards or samples. • Absorbent paper or paper toweling. • Distilled or deionized ultrapure water. 7. Technical Application Tips • Do not mix or substitute components from other kits. • To ensure the validity of experimental operation, it is recommended that pilot experiments using standards and a small selection of sample dilutions to ensure optimal dilution range for quantitation. www.avivasysbio.com – 888.880.0001 – [email protected] 4 • Samples exhibiting OD measurements higher than the highest standard should be diluted further in the appropriate sample dilution buffers. • Prior to using the kit, briefly spin component tubes to collect all reagents at the bottom. • Replicate wells are recommended for standards and samples. • Cover microplate while incubating to prevent evaporation. • Do not allow the microplate wells dry at any point during the assay procedure. • Do not reuse tips or tube to prevent cross contamination. • Avoid causing bubbles or foaming when pipetting, mixing or reconstituting. • Completely remove of all liquids when washing to prevent cross contamination. • Prepare reagents immediately prior to use and do not store, with the exception of the top standard. • Equilibrate all materials to ambient room temperature prior to use (standards exception). • For optimal results for inter- and intra-assay consistency, equilibrate all materials to room temperature prior to performing assay (standards exception). • Pipetting less than 1 µL is not recommended for optimal assay accuracy. • Once the procedure has been started, all steps should be completed without interruption. Ensure that all reagents, materials and devices are ready at the appropriate time. • Incubation times will affect results. All wells should be handled in the same sequential order and time intervals for optimal results. • Samples containing bilirubin, precipitates or fibrin strands or are hemolytic or lipemic might cause inaccurate results due to interfering factors. • TMB Substrate is easily contaminated and should be colorless or light blue until added to plate. Handle carefully and protect from light. 8. Reagent Preparation • Equilibrate all materials to room temperature prior to use and use immediately. 8.1. Standard 8.1.1. Prepare a fresh standard curve for each assay performed. Reconstituted standards cannot be stored for later use. For further directions, please refer to the Certificate of Analysis. 8.1.2. Gently spin the vial prior to use. Reconstitute the standard to a stock concentration of 10ng/ml using 1ml of sample diluent. Allow the standard to sit for a minimum of 10 minutes with gentle agitation prior to making dilutions. 8.2. 1X Biotinylated Anti-Human ULBP2 Antibody 8.2.1. Prepare the 1X Biotinylated Human ULBP2 Antibody immediately prior to use by diluting the 100X Biotinylated Human ULBP2 Antibody 1:00 with Antibody Diluent Buffer. 8.2.2. For each well strip to be used in the experiment prepare 100 µL by adding 1 µL of 100X Biotinylated Human ULBP2 Antibody to 99 µL Antibody Diluent Buffer. 8.2.3. Mix thoroughly and gently. Hold no longer than 2 hours prior to using in procedure. Do not store at 1X concentration for future use. 8.3. 1X Avidin-Biotin-Peroxidase Complex (ABC) www.avivasysbio.com – 888.880.0001 – [email protected] 5 8.3.1. Prepare the 1X Avidin-Biotin-Peroxidase Complex (ABC) immediately prior to use by diluting the 100X Avidin-Biotin-Peroxidase Complex (ABC) 1:100 with ABC Dilution Buffer. 8.3.2. For each well to be used in the experiment prepare 100 µL, by adding 1 µL of 100X Avidin- Biotin-Peroxidase Complex (ABC) to 99
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