Prevalence and Risk Factors of Human and Bovine Tuberculosis at Mymensingh District in Bangladesh by Md
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Global Journal of Medical Research: G Veterinary Science and Veterinary Medicine Volume 15 Issue 1 Version 1.0 Year 2015 Type: Double Blind Peer Reviewed International Research Journal Publisher: Global Journals Inc. (USA) Online ISSN: 2249-4618 & Print ISSN: 0975-5888 Prevalence and Risk Factors of Human and Bovine Tuberculosis at Mymensingh District in Bangladesh By Md. Abu Sayeed Sarker, Md. Siddiqur Rahman , Bhudeb Chandra Barman , Md. Emtiaj Alam, Md. Fashiur Rahman, & Roma Rani Sarker Bangladesh Agricultural University Abstract- Tuberculosis (TB) is a major global health problem and economically important zoonotic diseases worldwide. This study was conducted to determine the prevalence of tuberculosis and risk factor in human and cattle at Mymensingh district in Bangladesh. In this study, 3085 human and 649 cattle were examined during January 2009 to December 2011 at Dhobaura upazila of Mymensingh district. The overall prevalence of tuberculosis in human and animal was 9.7% and 2.34%, respectively (p<0.01). The difference in the prevalence of tuberculosis in human and cattle is statistically significant (p<0.001). Statistically significant higher prevalence was found in the age group of ≤2920 years and 30≤39 years than 40≤49 years, 50≤59 years and ≥60 years age group of human (p<0.001). Keywords: human, bovine, tuberculosis, prevalence, risk factor, Bangladesh. GJMR-G Classification : NLMC Code: WA 400 PrevalenceandRiskFactorsofHumanandBovineTuberculosisatMymensinghDistrictinBangladesh Strictly as per the compliance and regulations of: © 2015. Md. Abu Sayeed Sarker , Md. Siddiqur Rahman , Bhudeb Chandra Barman , Md. Emtiaj Alam, Md. Fashiur Rahman, & Roma Rani Sarker. This is a research/review paper, distributed under the terms of the Creative Commons Attribution- Noncommercial 3.0 Unported License http://creativecommons.org/licenses/by-nc/3.0/), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Prevalence and Risk Factors of Human and Bovine Tuberculosis at Mymensingh District in Bangladesh Md. Abu Sayeed Sarker α , Md. Siddiqur Rahman σ, Bhudeb Chandra Barman ρ, Md. Emtiaj AlamѠ, Md. Fashiur Rahman¥ & Roma Rani Sarker§ Abstract- Tuberculosis (TB) is a major global health problem causing disease in humans ((Dankner et al., 1993). M. and economically important zoonotic diseases worldwide. This bovis is the most universal pathogen among 2015 study was conducted to determine the prevalence of mycobacteria and affects many vertebrate animals of all tuberculosis and risk factor in human and cattle at age groups although, cattle, goats and pigs are found to Year Mymensingh district in Bangladesh. In this study, 3085 human be most susceptible, while sheep and horses are and 649 cattle were examined during January 2009 to 5 December 2011 at Dhobaura upazila of Mymensingh district. showing a high natural resistance. M. tuberculosis and The overall prevalence of tuberculosis in human and animal M. bovis are genetically and antigenically very similar was 9.7% and 2.34%, respectively (p<0.01). The difference in and cause identical clinical disease in humans. the prevalence of tuberculosis in human and cattle is ((Radostis et al., 2000; Zinsstag et al., 2006). statistically significant (p<0.001). Statistically significant higher Transmissions of tuberculosis in humans are mainly by prevalence was found in the age group of 20 ≤29 years and inhalation and ingestion of raw milk or unpasteurized 30≤39 years than 40≤49 years, 50≤59 years and ≥60 years dairy products or meat from an infected animal age group of human (p<0.001). The relationship among the ((Srivastava et al., 2008). Aerosol exposure to M. bovis prevalence in different age group of cattle was statistically insignificant (p=0.129). In human, statistically significant is considered to be the most frequent route of infection higher prevalence was recorded in female (11.2%) than in of cattle, but infection by ingestion of a contaminated male (8.6%) (p=0.02). But in cattle, statistically insignificant material also occurs ((Biet et al., 2005). Fever, night slightly higher prevalence was recorded in male (2.4%) than in sweats, weight loss, poor appetite, weakness, chest female (2.1%) (p=0.777). In human, highest prevalence was pain, swollen glands and breathing problems, a general Volume XV Issue 1 Version I found in the April month (15%) and lowest in the July month sick feeling are the general symptom in human. In cattle, ) D D (5.7%) (p=0.012). In cattle, highest prevalence was found in DD the early stages of TB, clinical signs are not visible. In G the April and October month (3.8%) and no positive cases later stages, clinical signs may include: emaciation, ( were recorded in the July month. lethargy, weakness, anorexia, low-grade fever, and Keywords: human, bovine, tuberculosis, prevalence, risk pneumonia with a chronic, moist cough. Lymph node factor, bangladesh. enlargement may also be present ((Radostis et al., I. Introduction 2000). The most common method for diagnosing TB in Research human worldwide is sputum smear microscopy uberculosis (TB) remains is a major global health (developed more than 100 years ago). Chest x-ray also problem (Cosivi et al., 1998; Schiller et al., 2010). a common method for TB diagnosis in human. In Medical TTuberculosis plays a central role in public health countries with more developed laboratory capacity, and animal health because of its severe disease in cases of TB are also diagnosed via culture methods. TB humans and significant economic losses to cattle in Bangladesh is commonly diagnosed by suggestive producers related to affected herds (Rodriguez et al., clinical symptoms and signs coupled with a suggestive 1999; Ayele, et al., 2004; Zinsstag et al., 2006; Samad, chest x-ray and sputum sample (Matin et al., 2011). 2008; OIE, 2009). Mycobacterium tuberculosis, M. bovis Bovine TB is difficult to diagnose with clinical signs alone. Many methods are available for diagnosis of Global Journal of Author § : Department of Medicine, Faculty of Veterinary Science, tuberculosis in infected animals but the single Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. comparative intradermal tuberculin test (SCITT) is most Author α : Livestock Officer,Department of Livestock Services Government of the Peoples Republic of Bangladesh and PhD fellow, widely used for diagnosis and eradication of Bovine Department of Medicine Bangladesh Agricultural University, tuberculosis (OIE, 2009). In Bangladesh, so far the Mymensingh Bangladesh. e-mail: [email protected] single intradermal (SID) skin test with purified protein Author σ ρ : Department of poultry Science, Faculty of Animal derivative (PPD) has been used to detect the prevalence Husbandry, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh. of bovine TB (BTB) ((Pharo et al., 1981; Samad and Author Director Mymensingh Medical college Hospital, Rahman, 1986; Islam et al., 2007). Sero-diagnostic ¥ Ѡ : Mymensingh, Bangladesh. tests, ICGA as Antigen Rapid Bovine TB Ab Test Kit was ©2015 Global Journals Inc. (US) Prevalence and Risk Factors of Human and Bovine Tuberculosis at Mymensingh District in Bangladesh used to detect Bovine TB and its effect on milk animals, the data were collected in different months of production in lactating cows in Bangladesh ((Rahman the year. and Samad, 2008). c) Caudal fold tuberculin (CFT) test Tuberculosis causes ill-health among millions of This is the primary screening test to identify people each year and ranks as the second leading animal potentially infected with bovine TB. The test cause of death from an infectious disease worldwide. measures the immune response to Mycobacterium The latest estimates included that there were almost 9 bovis, the causative agent of bovine TB. The test was million new cases in 2011 and 1.4 million TB deaths performed by intradermal injection of 0.1 ml bPPD with a worldwide. About 89% of the world’s TB cases are hypodermic syringe in the skin of the caudal fold (the account for 96 countries. Bangladesh ranks 5th Globally fold of skin at the base of the tail). If the animal was (WHO, 2006). In Bangladesh mortality rate in human exposed to mycobacteria, the immune system varies from 19 to 82 (average 45), Prevalence varies responded with inflammatory cells at the injection site to from 199 to 698 (average 411), incidence varies from cause swelling and/or discoloration of the skin. After 72 2015 185 to 268 (average 225) per 100 000 population (WHO, hours, inspection and palpation of the injection site was 2012). There is little literature available on the prevalence done to evaluate for a response. Marked edematous Year of tuberculosis in human and animal in Bangladesh. swelling, reddening at the injection site classified the Therefore, the study was conducted to determine the 6 animal as a responder. If no response was noted, the prevalence and risk factors associated with human and animal was classified as CFT test-negative. Responder bovine tuberculosis. animals were further tested with CCT test for II. Methodology confirmation. This was a prospective, cross-sectional, d) Statistical analysis observational study conducted among the human and The collected data was compiled, tabulated and animal population simultaneously. The study was analyzed in accordance with the objectives of the study. conducted for the period of three years starting from The approximate percentage was calculated for each January 2009 to December 2011 to determine the parameter. The questionnaire-based data was prevalence and risk factors associated with human and processed in Microsoft Excel and analyzed in SPSS. The bovine tuberculosis. z-test for proportions was done to find out the relationship of different factors on the occurrence of a) Selection of study population tuberculosis in human and cattle. Where Significance Volume XV Issue 1 Version I In this study, 3085 patients who were admitted was determined in terms of age, sex, year and month of ) in Dhobaura Health Complex and 649 cattle registered DDDD occurrence at 5% level. G ( in Dhobarua Upazila Veterinary Hospital were selected.