Survey and Spatial Distribution of Shoreline Malacofauna in Grande Island, Subic Bay
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Philippine Journal of Science 139 (2): 149-159, December 2010 ISSN 0031 - 7683 Survey and Spatial Distribution of Shoreline Malacofauna in Grande Island, Subic Bay Gizelle A. Batomalaque*, Bryan Geoffrey P. Arce, Ma. Brenda M. Hernandez, and Ian Kendrich C. Fontanilla Institute of Biology, Quirino St., University of the Philippines, Diliman, Quezon City 1101 Grande Island, Subic Bay is an excellent site for studying spatial distributions on a local scale because of its topographic heterogeneity. This study aimed to provide a systematic survey and spatial distribution of shoreline malacofauna of the island using a total of 956 continuous 2 x 2 m plots. Substrate types were classified as sand, sand-pebble, rock-sand, rock-pebble, pebble- rock-coral rubble, boulder, and wood. One hundred mollusk species were identified from 44,465 individuals with 54 additional individuals still unresolved. Mollusks were found to be distributed according to the substrate type (composition and particle size) and are independent of the total area sampled. Substrates that are loose or unstable tend to have only few species while those that are compact have more species. Only bivalves were found in the sand substrate. Planaxids, nerites, trochids, and siphonarids were the dominant groups in substrates with rocks, pebbles, and coral rubble. Nudibranchs only occurred in rocky substrates. Nacellids and littorinids predominate particularly in boulders. Littorinids were also numerous in woody substrates. Juvenile trochids and planaxids were most abundant in regions with calm water while Cellana species and adult trochids were particular in areas exposed to strong currents. The morphological adaptations of different species enabled them to occur in specific habitat types. Key Words: mollusk, shoreline survey, spatial distribution, substrate type INTRODUCTION testaceos de las Islas Filipinas, Jolo y Marianas (1904- 1905), relying heavily on the records of Cumming, Survey of molluscan species has been a relevant tool included 3,121 species (Springsteen and Leobrera 1986). for storing and organizing taxonomic data, which could More recent publications of mollusk listings include those provide insights on the molluscan distribution of a of Springsteen and Leobrera (1986) and Poppe (2008a; particular area for potential economic, scientific and even 2008b). At present, no systematic studies have been medical applications. The global estimate of described executed on the biodiversity of marine mollusks around molluscan species is 70,000 (IUCN 2004), and around Luzon as confirmed by the scarcity of published literature. 22,000 species are believed to be present in the Philippines There is, however, one study in Mindoro Island conducted (PBCPP 2002). Mollusks are the second most diverse by Berdach (1981) that focused on the inventory of marine invertebrates in the Philippines, with an estimated species gastropods in the Man and Biosphere (MAB) Reserve endemism of 2-4% (PBCPP 2002). Much of the published area in Puerto Galera where Berdach (1981) found a total information regarding Philippine molluscan species is of 343 species from 45 families. Nevertheless, Berdach based on collections made by Hugh Cumming between (1981) failed to mention the sampling effort in terms of 1836 and 1840. Hidalgo’s Catalogo de los Molluskos the total area surveyed. Furthermore, he also included *Corresponding author: [email protected] dead specimens (i.e. shells only) in his inventory because he assumed these specimens inhabited the area. Studies 149 Philippine Journal of Science Batomalaque et al.: Survey and Spatial Vol. 139 No. 2, December 2010 Distribution of Shore Mollusks of molluscan diversity in the Philippines were mainly to the substrate type. Temporal distribution was not studied focused on regional scales according to ocean basins due to limited data-gathering time and less on local scales according to physical gradients (Vallejo 2001). Little is known of the correlation between habitat types and species composition on a smaller scale. MATERIALS AND METHODS Determining spatial distribution patterns among mollusk species is important in studying the ecology of these species. An accurate description of these patterns Species survey also provides an index for studying relationships and All five sides of the island were surveyed. Sampling was interactions between different mollusks (Springsteen and conducted from May 20 to 22 and May 26 to June 1, 2008 Leobrera 1986). These also reflect how abiotic and biotic during low tide, since the area of interest was the subtidal factors collectively affect species zonations (Azouzi et zone (zone always submerged in water even at low tide). al. 2002). Abiotic factors identified are substrate grain Systematic snorkel-surveys were done in continuous 2 x 2 m size, wave action, water content, and temperature and plots along the edge of the shoreline around nearly the whole salinity of interstitial water (water contained in crevices stretch of the island. The plots were bounded by standard and within the particles of the substrate). Among these, PVC pipes that could sink under water. A maximum of 10 De Arruda and Amaral (2003) pointed to morphodynamic minutes per plot was allotted to search for mollusks. Pebbles states (substrate types and wave action) as the primary and rocks were moved and replaced in order to thoroughly determinant of distribution patterns in environments with survey each plot. For sandy substrates, the surface was a variation of physical characteristics. Biotic factors, slightly swept or dug up for burrowing mollusks. on the other hand, include recruitment (addition of Habitat description was limited only to the type of members to the population or community), predation, substrate. This was characterized according to particle and competition. Together with interactions between size using a modified Wentworth Grain Size Classification abiotic components and biotic relationships, species (El-Shaarawi and Piegorsh 2002): sand (<1 cm), pebble characteristics such as size, feeding habits, mobility, and (1-6.4 cm), rock (rubble/cobble) (6.4-25.6 cm), boulder morphology also determine marine molluscan distribution. (>25.6 cm). In the presence of mixed substrate types, a It is acknowledged that species distribution is shaped by plot was assigned to a particular substrate if that substrate the interplay between these factors at different extents comprised more than 50% of the entire plot. The water and never attributable solely to a single factor (De Arruda movement or current intensity was noted for each side and Amaral 2003). of the island. Furthermore, the presence of associated Grande Island just off the coast of Subic Bay, Zambales fauna such as echinoderms, arthropods, and macroalgae (14˚46’25.40” N, 120˚13’53.45” E, total land area were noted. of 40 hectares and perimeter of 2.2 km) provides an Only live mollusks were considered in the study. For excellent site for studying habitat associations because every plot, the number of individuals of each species the substrate types found in the perimeter of the island encountered was recorded. A voucher specimen from are heterogeneous. The five sides of the island include each encountered species was brought to the laboratory different types of substrates: fine sand, coral rubble, for identification. All mollusks were identified down to pebbles, cobbles, and boulders. Water movements at the species level, where possible, using Marsh (1964), different sides also show variation. Hinton (1972), Dance (1976), Springsteen and Leobrera This study aimed to determine the molluscan species (1986), Abbott (1991), and Poppe (2008a) as references. composition of the subtidal region around Grande Island The soft tissues were then removed and the shells cleaned. using continuous 2 x 2 m quadrats. That is, the subtidal Photographs of the shells were then taken. region examined was within 2 m from the waterline at low tide. Other objectives of this study include the Data analysis determination of the relative abundance of species in The relative abundance (RA) of species in each substrate different habitats, the comparison of species composition type was determined. Comparison of species composition of marine mollusks in different substrate types, and the between sites was facilitated using Sorenson’s Similarity correlation of mollusk morphology with the habitat type. Index (S) based on the presence or absence of species in Since no previous published studies on mollusks have a particular substrate type, which is given by the equation been conducted on Grande Island, baseline data from S (index of similarity) = 2C/ A+B, where A is the number this study would certainly prove useful for future studies. of species in the first substrate type; B is the number of Though several factors could affect the spatial distribution species in the second substrate type; and C is the number and assemblage of marine mollusks, this study was limited of species common to both substrate types. A low index 150 Philippine Journal of Science Batomalaque et al.: Survey and Spatial Vol. 139 No. 2, December 2010 Distribution of Shore Mollusks value means a large difference in species composition 86 species from 24 families from class Gastropoda, and between the two substrate types compared. Lastly, a 14 species from 11 families from class Bivalvia. Another dendrogram was generated from the distances (1-S) using 54 individuals were unidentified, including one juvenile Unweighted Pair-Group Method with Arithmetic