DOCSLIB.ORG

The Use of Cytogenetic Tools for Studies in the Crop-To-Wild Gene Transfer Scenario

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Use of Cytogenetic Tools for Studies in the Crop-To-Wild Gene Transfer Scenario Cytogenetics and Plant Breeding Cytogenet Genome Res 120:384–395 (2008) DOI: 10.1159/000121087 The use of cytogenetic tools for studies in the crop-to-wild gene transfer scenario a a b b E. Benavente M. Cifuentes J.C. Dusautoir J. David a Departamento de Biotecnología, ETS Ingenieros Agrónomos, Universidad Politécnica de Madrid, Madrid (Spain) b Unité Mixte de Recherche Diversité et Adaptation des Plantes Cultivées (UMR-DIAPC), Supagro Montpellier-INRA-IRD, Montpellier (France) Accepted in revised form for publication by M. Schmid, 7 December 2007. Abstract. Interspecific hybridization in plants is an im- into a recipient species (i.e., the wild). Several examples in portant evolutionary phenomenon involved in the dynam- hybrids and derivatives are given to illustrate how these ap- ics of speciation that receives increasing interest in the con- proaches may be used to evaluate the potential for gene text of possible gene escapes from transgenic crop varieties. transfer between crops and wild relatives. Different tech- Crops are able to cross-pollinate with a number of wild re- niques, from classical chromosome staining methods to re- lated species and exchange chromosome segments through cent developments in molecular cytogenetics, can be used homoeologous recombination. In this paper, we review a set to differentiate genomes and identify the chromosome re- of cytogenetic techniques that are appropriate to document gions eventually involved in genetic exchanges. Some clues the different steps required for the stable introgression of a are also given for the study of fertility restoration in the in- chromosome segment from a donor species (i.e., the crop) terspecific hybrid forms. Copyright © 2008 S. Karger AG, Basel Observation of interspecific hybrids between different was kept isolated within each species and for a gene ex- taxa in nature is rare but recurrent (Ellstrand et al., 1996; changed after their evolutionary differentiation can be in- Arnold, 1997). Most often, such hybrids are almost fully consistent. These phylogenetic incongruities support a re- sterile and therefore were long considered as dead end forms. ticulate evolution pattern (Vriesendorp and Bakker, 2005; Nowadays, the new technological tools permit us to inves- Marhold and Lihova, 2006). tigate whether a majority of plant species may be derived Following hybridization, gene flow may result in the in- from past hybridization events (e.g., Stebbins, 1958) and this trogression of chromosome segments from one species to is acknowledged to be an important phenomenon in angio- another by direct and recurrent back crosses. Alternatively, sperm evolution (Rieseberg and Ellstrand, 1993; Rieseberg interspecific hybrids might give birth to new fertile species and Wendel, 2004). The reality of ancient introgression is either via spontaneous and instantaneous chromosome difficult to establish but phylogenetic studies are increas- doubling (allopolyploidy; e.g., wheat and oilseed rape) or via ingly providing indirect evidence. Interspecific gene trans- the fixation of viable recombinant chromosome sets (homo- fers cancel the accumulated nucleotide divergences between ploidy; see Rieseberg, 1997; Rieseberg and Carney, 1998). species. Then the phylogenetic trees obtained for a gene that These stable and fertile allopolyploid and homoploid forms can also constitute bridges and gene reservoirs for subse- quent gene flows back to their diploid progenitors. Introgression can then act on ecosystems by species in- The authors are funded by the ‘ACI Impact des OGM’ from the French Ministry vasion or replacement (Ellstrand, 2003) and may thus have of Higher Education and Research. an impact in biological diversity. In the crop-wild context, Request reprints from E. Benavente with or without genetically transformed varieties, evolution Departamento de Biotecnología (Genética), ETSI Agrónomos towards nasty weeds is also a major rising concern (Ell- Universidad Politécnica de Madrid, 28040–Madrid (Spain) telephone: +34 91 3365714; fax: +34 91 5434879 strand et al., 1999; Burke et al., 2002; Lu and Snow, 2005). e-mail: [email protected] Weeds incorporating transgenes carrying insect or disease Fax +41 61 306 12 34 © 2008 S. Karger AG, Basel Accessible online at: E-Mail [email protected] 1424–8581/08/1204–0384$24.50/0 www.karger.com/cgr www.karger.com resistance, or resistance to environmental stresses, are like- 1 ly to display increased overall fitness and competitiveness, Crop Wild relative and their spread may have serious agronomic and environ- mental implications (Warwick et al., 2003; Watrud et al., 2004; Reichman et al., 2006). Natural selection of a favour- Interspecific hybrid able allele in an interspecific context has long been neglect- 2 ed but while the spread of an introgressed allele is possible through neutral evolution, it can thrive much more success- Homoeologous pairing fully through natural selection even with low selective ad- Intergenomic recombination vantage (Arnold et al., 1999; Rieseberg and Burke, 2001). Wild species and crops are growing in sympatry in many 3 Reductional Non-reductional documented situations (see Ellstrand et al., 1999 for a re- meiosis meiosis view). In these complexes of species, either the direct wild ancestor of the crop or any related species could be a pos- Aneuploid gametes Unreduced gametes sible recipient of the crop genes. The probability of interspe- cific hybridization depends on various factors, such as phy- 2n 2n logenetic relatedness, mating systems, and density and spa- tial distribution of wild relatives (Warwick et al., 2003; Chevre et al., 2003). Nevertheless, the main goal is not to prove that interspecific hybridization occurs in nature and Backcross Selfing may impact the species communities, but to document the Hybrid offspring likelihood of chromosome segment transmission and to de- 4 scribe the ways of gene exchange and transfer between spe- cies. Figure 1 illustrates the various pathways that a crop nu- cleotide sequence must follow to be stably incorporated into G a wild related species. In this paper we review the utility of n cytological methodologies to investigate the critical steps in Backcross the route and to identify the mechanisms and the fate of a transferred chromosome segment. Gn T h e t o o l s Stable introgression into the wild species This section briefly describes the most common meth- ods used for chromosome analysis in interspecific hybrids Fig. 1. Steps in the crop-to-wild gene flow route. 1: Interspecific and their progenies. hybridization. 2: Chromatid exchange between crop and wild chromo- somes. 3: Production of functional gametes and fertilization. 4: Trans- mission of crop-wild recombinant chromosomes during karyotype Classical cytogenetic methods evolution. The red block represents a crop genetic sequence success- Traditional dyes (aceto-carmine, aceto-orcein, Feulgen fully introgressed, whereas the blue block represents a non-recombined and variants) bind chromatin and nicely stain chromo- sequence that will likely be lost during karyotype evolution in the hy- somes for optical microscopy. Despite the great develop- brid lineage. ment of newer alternatives, these staining methods are still commonly used to visualize chromosomes both in mitotic and meiotic cells. Their main limitation is that individual phase-I (MI) cells due to the different morphology of chro- chromosomes within a complement cannot be identified mosomes at this meiotic stage. This explains why many re- unless they differ morphologically, i.e., by size, centromere ports that document the employment of C-banding for position, presence or location of secondary constrictions, meiotic MI pairing analyses have not used the technique to etc. identify specific chromosomes but to simply discriminate Several staining methods provide distinctive and repro- genomes with sharply distinct banding patterns (e.g., ducible banding patterns for specific chromosomes or ge- Hutchinson et al., 1983). nomes based on the size and location of different classes of chromatin. Among the classical banding methods, C-band- Cytomolecular methods ing has been the most frequently used for identification of Fluorescent in situ hybridization (FISH), using different individual plant chromosomes either at mitosis or meiosis classes of DNA sequences as probes, has been extensively (e.g., Dhaliwal et al., 1977; Gill et al., 1991) ( Fig. 2 ). It is worth used for cytological discrimination of specific chromo- mentioning that a perfectly distinctive band pattern on mi- somes and individual genomes in many plant species (Jiang totic karyotypes may not be as easily resolved on meta- and Gill, 1994, 2006). The following sections will provide Cytogenet Genome Res 120:384–395 (2008) 385 numerous references illustrating the use of different FISH approaches in interspecific hybrids and derivatives from most of the crops for which gene flow to wild relatives has been documented (wheat, rice, oilseed rape, cotton or sun- flower, among others). FISH using total genomic DNA labelled probes (GISH) easily and reliably informs on the genome adscription of specific chromosomes (or chromosomal segments) in many multigenomic materials based on colour codes ( Figs. 3 –5). The technique is sometimes referred to as multicolor-GISH when differentially labelled genome probes are simultane- ously hybridized to chromosome spreads. In some instanc- es, the presence of shared sequences in the target and non ! target genomes results in cross-hybridization signals of to- Fig.
Load more

Recommended publications
  • Identify New Resistant Genes for Eyespot Diseases of Wheat In
    IDENTIFICATION AND MAPPING OF RESISTANCE GENES FOR EYESPOT OF WHEAT IN AEGILOPS LONGISSIMA By HONGYAN SHENG i A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy WASHINGTON STATE UNIVERSITY Department of Plant Pathology May 2011 © Copyright by HONGYAN SHENG, 2011 All Rights Reserved To the Faculty of Washington State University: The members of the Committee appointed to examine the dissertation of HONGYAN SHENG find it satisfactory and recommend that it be accepted. _________________________________________ Timothy D. Murray, Ph. D., Chair _________________________________________ Xianming Chen, Ph. D. _________________________________________ Scot H. Hulbert, Ph. D. _________________________________________ Tobin L. Peever, Ph. D. _________________________________________ Stephen S. Jones, Ph. D. ii ACKNOWLEDGMENT I would like to express my sincere gratitude and appreciation to my mentor and major advisor, Dr. Timothy D. Murray, for all his guidance, support, patience, and encouragement throughout my entire Ph. D. process at Washington State University. I am grateful to Dr. Murray for sharing his knowledge of plant pathology, providing insight into this dissertation, and leading me to the complex and fascinating world of genetics. My grateful appreciation goes to my committee members, Dr. Tobin L. Peever, Dr. Xianming Chen, Dr. Scot H. Hulbert, and Dr. Stephen S. Jones for their helpful advice and guidance during my graduate work and critical review of my dissertation. I would especially like to thank Dr. Deven R. See (USDA-ARS Regional Small Grains Genotyping Laboratory at Pullman, WA) for providing techniques and equipments for marker analysis work. Most of all, I am grateful for his critical suggestion leading to successful results.
    [Show full text]
  • Evaluation of Genetic Diversity of Algerian Aegilops Ventricosa Tausch
    World Journal of Environmental Biosciences All Rights Reserved WJES © 2014 Available Online at: www.environmentaljournals.org Volume 8, Issue 1: 1-6 ISSN 2277- 8047 Evaluation of Genetic Diversity of Algerian Aegilops Ventricosa Tausch. Using Inter- Simple Sequence Repeat (ISSR) Markers Mohammed Chekara Bouziani1, 2, 3*, Sakina Bechkri3, Ines Bellil3, Douadi Khelifi3 1 Faculty of Natural Sciences and Life, Ferhat Abbas University, Setif 1, Algeria. 2 Faculty of Exact Sciences and Natural Sciences and Life, Oum El Bouaghi University, Algeria. 3 Laboratory of Genetic Biochemistry and Plant Biotechnology, Constantine 1 University, Algeria. ABSTRACT The genetic polymorphism of thirty Aegilops ventricosa Tausch. accessions sampled from northern Algeria was evaluated using inter-simple sequence repeat (ISSR) markers (into 5’). Six reproducible primers were used, and 53 out of 62 amplified distinct bands were found with a high score of polymorphism (87.48%). A significant number of specific bands was identified which were useful for genotyping. The majority of ISSR primers showed important resolving power with an average of 10.86, reflecting high informative markers tested in this investigation. SIMQUAL coefficient was used to calculate the genetic similarity among the 30 accessions. This similarity ranged from 0.5806 to 1. Based on Unweighted Pair-Group Method using Arithmetic Averages (UPGMA) method, a dendrogram was established to assess the intraspecific relationships via the genetic distances between the 30 accessions. The ISSR results showed a high variability in the studied collection. These markers could be efficiently applied to estimate the genetic diversity in the Ae. venticosa or others species in the Aegilpos genus. Keywords: Aegilops ventricosa Tausch., Genetic diversity, ISSR markers, Northern Algeria.
    [Show full text]
  • Update on the Genomics and Basic Biology of Brachypodium
    Forum Update on the genomics and basic biology of Brachypodium International Brachypodium Initiative (IBI) 1,2* 3 4 5 Pilar Catalan , Boulos Chalhoub , Vincent Chochois , David F. Garvin , 6 7 8 9 Robert Hasterok , Antonio J. Manzaneda , Luis A.J. Mur , Nicola Pecchioni , 10 11 12,13 Søren K. Rasmussen , John P. Vogel , and Aline Voxeur 1 Department of Agricultural and Environmental Sciences, High Polytechnic School of Huesca, University of Zaragoza, Carretera Cuarte km 1, 22071 Huesca, Spain 2 Department of Botany, Institute of Biology, Tomsk State University, Lenin Avenue 36, Tomsk 634050, Russia 3 Unite´ de Recherche en Ge´ nomique Ve´ ge´ tale (URGV), Institut National de la Recherche Agronomique (INRA)–Centre National de la Recherche Scientifique (CNRS)–Universite´ Evry-Val d’Essonne (UEVE), Organization and Evolution of Plant Genomes (OEPG), 91057 Evry CEDEX, France 4 Commonwealth Scientific and Industrial Research Organisation (CSIRO) Plant Industry, Black mountain laboratories, Clunies Ross Street, 2601 Acton, Canberra, Australia 5 United States Department of Agriculture (USDA)–Agricultural Research Service (ARS) Plant Science Research Unit, 411 Borlaug Hall, University of Minnesota, 1991 Upper Buford Circle, St Paul, MN 55108, USA 6 Department of Plant Anatomy and Cytology, University of Silesia in Katowice, Jagiellonska 28, 40-032 Katowice, Poland 7 Departamento Biologı´a Animal, Biologı´a Vegetal y Ecologı´a, Universidad de Jae´ n, Paraje las Lagunillas s/n, 23071 Jae´ n, Spain 8 Aberystwyth University, Institute of Biological,
    [Show full text]
  • The Wonderful World of Chromosomes Pat Heslop-Harrison DNA and Botany
    The wonderful world of chromosomes Pat Heslop-Harrison www.molcyt.com DNA and botany • Richard Gornall • All you ever wanted to know about DNA and Plant Genetics, but didn’t like to ask! • • Clive Stace • Classification by molecules: what’s in it for field botanists Darwin: The final paragraph of “The Origin” It is interesting to contemplate a tangled bank, clothed with many plants of many kinds, with birds singing on the bushes, with various insects flitting about, and with worms crawling through the damp earth, and to reflect that these elaborately constructed forms, so different from each other, and dependent upon each other in so complex a manner, have all been produced by laws acting around us.. Darwin:Darwin:Darwin: The The The final final final paragraph paragraph paragraph ofofof “The “The “The Origin” Origin” Origin” • It is interesting to contemplate … many plants of many kinds … and to reflect that these elaborately constructed forms, so different from each other … .have all been produced by laws acting around us … from so simple a beginning endless forms most beautiful and most wonderful have been, and are being evolved. Crop plants • Little interest to BSBI as few are native to UK Major Crops 2007 million tons production (sugar cane excluded) Crop plants • Farmers have been getting better for 5000 years … – Weed control – Drainage/irrigation – Fertilization Crop plants • Great interest to BSBI as – Dominant in the landscape, particularly in England – Crop cultivation has the major impact on biodiversity – Wild collection of
    [Show full text]
  • Developing a High-Throughput SNP-Based Marker System to Facilitate the Introgression of Traits from Aegilops Species Into Bread Wheat (Triticum Aestivum)
    ORIGINAL RESEARCH published: 24 January 2019 doi: 10.3389/fpls.2018.01993 Developing a High-Throughput SNP-Based Marker System to Facilitate the Introgression of Traits From Aegilops Species Into Bread Wheat (Triticum aestivum) Alexandra M. Przewieslik-Allen 1*, Amanda J. Burridge 1, Paul A. Wilkinson 1, Mark O. Winfield 1, Daniel S. Shaw 1, Lorna McAusland 2, Julie King 2, Ian P. King 2, Keith J. Edwards 1 and Gary L. A. Barker 1 1 Life Sciences, University of Bristol, Bristol, United Kingdom, 2 Plant Sciences, Sutton Bonington Campus, Leicestershire, United Kingdom The genus Aegilops contains a diverse collection of wild species exhibiting variation Edited by: in geographical distribution, ecological adaptation, ploidy and genome organization. István Molnár, Centre for Agricultural Research Aegilops is the most closely related genus to Triticum which includes cultivated wheat, (MTA), Hungary a globally important crop that has a limited gene pool for modern breeding. Aegilops Reviewed by: species are a potential future resource for wheat breeding for traits, such as adaptation Parveen Chhuneja, Punjab Agricultural University, India to different ecological conditions and pest and disease resistance. This study describes Kentaro Yoshida, the development and application of the first high-throughput genotyping platform Kobe University, Japan specifically designed for screening wheat relative species. The platform was used to Pilar Hernandez, Instituto de Agricultura Sostenible screen multiple accessions representing all species in the genus Aegilops. Firstly, the (IAS), Spain data was demonstrated to be useful for screening diversity and examining relationships *Correspondence: within and between Aegilops species. Secondly, markers able to characterize and track Alexandra M.
    [Show full text]
  • Reevaluation of Systematic Relationships in Triticum L. and Aegilops L
    AN ABSTRACT OF THE THESIS OF Laura A. Morrison for the degree of Doctor of Philosophy in Botany and Plant Pathology presented on October 21, 1994. Title: Reevaluation of Systematic Relationships in Triticum L. and Aegilops L. Based on Comparative Morphological and Anatomical Investigations of Dispersal Mechanisms Redacted for Privacy Abstract approved: Kenton L. Chambers Comparative morphological and anatomical studies of the dispersal mechanisms characterizing the wheat complex (Triticum L. and Aegilops L.) have documented patterns of adaptive radiation which may have significance for evolutionary relationships. These patterns, which form an array of diverse types of diaspores among the diploid taxa, appear conceptually to have a starting point in the dimorphic inflorescence of Ae. speltoides. Separate dispersal trends, centered primarily in features of rachis disarticulation, lead in the direction of novel diaspore types for Aegilops and in the direction of domestication for Triticum. With respect to the taxonomy, this structural evidence supports the traditional Linnaean generic circumscriptions and suggests a need for a monographic revision of Triticum. In documenting the dispersal mechanisms, these studies have clarified conventional interpretations and have offered new insights on the developmental relationships linking the wild and domesticated taxa of the wheat complex. Although genetic studies were not encompassed within this research, a consideration of the genetic explanations for rachis disarticulation and glume closure suggests that the phenotypic traits typically used in genetic studies are not well understood. Given that the reticulate nature of genomic relationships in the wheats is coupled with intergrading variation and polymorphic species, a proposal is made for a broader evolutionary view than is found in the strict cladistic concept.
    [Show full text]
  • Developing a High-Throughput SNP-Based Marker System to Facilitate the Introgression of Traits from Aegilops Species Into Bread Wheat (Triticum Aestivum)
    Przewieslik-Allen, A. M., Burridge, A. J., Wilkinson, P. A., Winfield, M. O., Shaw, D. S., McAusland, L., King, J., King, I. P., Edwards, K. J., & Barker, G. L. A. (2019). Developing a high-throughput SNP-based marker system to facilitate the introgression of traits from Aegilops species into bread wheat (Triticum aestivum). Frontiers in Plant Science, 9(1993), [1993]. https://doi.org/10.3389/fpls.2018.01993 Publisher's PDF, also known as Version of record License (if available): CC BY Link to published version (if available): 10.3389/fpls.2018.01993 Link to publication record in Explore Bristol Research PDF-document This is the final published version of the article (version of record). It first appeared online via Frontiers at DOI: 10.3389/fpls.2018.01993. Please refer to any applicable terms of use of the publisher. University of Bristol - Explore Bristol Research General rights This document is made available in accordance with publisher policies. Please cite only the published version using the reference above. Full terms of use are available: http://www.bristol.ac.uk/red/research-policy/pure/user-guides/ebr-terms/ ORIGINAL RESEARCH published: 24 January 2019 doi: 10.3389/fpls.2018.01993 Developing a High-Throughput SNP-Based Marker System to Facilitate the Introgression of Traits From Aegilops Species Into Bread Wheat (Triticum aestivum) Alexandra M. Przewieslik-Allen 1*, Amanda J. Burridge 1, Paul A. Wilkinson 1, Mark O. Winfield 1, Daniel S. Shaw 1, Lorna McAusland 2, Julie King 2, Ian P. King 2, Keith J. Edwards 1 and Gary L. A. Barker 1 1 Life Sciences, University of Bristol, Bristol, United Kingdom, 2 Plant Sciences, Sutton Bonington Campus, Leicestershire, United Kingdom The genus Aegilops contains a diverse collection of wild species exhibiting variation Edited by: in geographical distribution, ecological adaptation, ploidy and genome organization.
    [Show full text]
  • Manual on National Herbarium of Cultivated Plants
    Manual on National Herbarium of Cultivated Plants Division of Plant Exploration and Germplasm Collection ICAR-National Bureau of Plant Genetic Resources, New Delhi-110 012 Disclaimer: This document has been prepared primarily based on work done in the NHCP for past three decades with experience by the herbarium staff. No part of this of this document may be used without permission from the Director. Citation: Pandey Anjula, K Pradheep and Rita Gupta (2015) Manual on National Herbarium of Cultivated Plants, National Bureau of Plant Genetic Resources, New Delhi, India, 50p. © National Bureau of Plant Genetic Resources, New Delhi- 110 012, India About the Manual on Herbarium of Cultivated Plants The manual on ‘National Herbarium of Cultivated Plants’ contains information on National Herbarium of Cultivated Plants (NHCP) along with detailed guidelines on ‘How to use the NHCP’. Some practical experiences gathered while working in this facility are also included in the relevant context. Significant output from this facility in relevance of Plant genetic resource is enlisted. Knowledge on various aspects of the herbarium, need based demonstrations and user guidelines were disseminated in various training programmes conducted by ICAR-NBPGR to address various issues. To bring out this manual in present form is an attempt keeping in view various indentors approaching this facility from time to time to satisfied their quarries pertaining to consultation and use. Because of dependency of many users from various inter- disciplinary science especially from agriculture and pharmaceutical sciences, need was felt to develop this manual on NHCP. While developing this efforts have been made to include all the information in simple and user friendly way for benefit of users.
    [Show full text]
  • The Use of Cytogenetic Tools for Studies in the Crop-To-Wild Gene Transfer Scenario
    The use of cytogenetic tools for studies in the crop-to-wild gene transfer scenario E. Benavente M. Ofuentes J.C. Dusautoir J. David Departamento de Biotecnologia, ETS Ingenieros Agronomos, Universidad Politecnica de Madrid, Madrid (Spain) Unite Mixte de Recherche Diversite et Adaptation des Plantes Cultivees (UMR-DIAPC), Supagro Montpellier-INRA-IRD, Montpellier (France) Abstract. Interspecific hybridization in plants is an im­ into a recipient species (i.e., the wild). Several examples in portant evolutionary phenomenon involved in the dynam­ hybrids and derivatives are given to illustrate how these ap­ ics of speciation that receives increasing interest in the con­ proaches may be used to evaluate the potential for gene text of possible gene escapes from transgenic crop varieties. transfer between crops and wild relatives. Different tech­ Crops are able to cross-pollinate with a number of wild re­ niques, from classical chromosome staining methods to re­ lated species and exchange chromosome segments through cent developments in molecular cytogenetics, can be used homoeologous recombination. In this paper, we review a set to differentiate genomes and identify the chromosome re­ of cytogenetic techniques that are appropriate to document gions eventually involved in genetic exchanges. Some clues the different steps required for the stable introgression of a are also given for the study of fertility restoration in the in­ chromosome segment from a donor species (i.e., the crop) terspecific hybrid forms. Observation of interspecific hybrids between different was kept isolated within each species and for a gene ex­ taxa in nature is rare but recurrent (Ellstrand et al., 1996; changed after their evolutionary differentiation can be in­ Arnold, 1997).
    [Show full text]
  • Dated Tribe-Wide Whole Chloroplast Genome Phylogeny Indicates
    Bernhardt et al. BMC Evolutionary Biology (2017) 17:141 DOI 10.1186/s12862-017-0989-9 RESEARCHARTICLE Open Access Dated tribe-wide whole chloroplast genome phylogeny indicates recurrent hybridizations within Triticeae Nadine Bernhardt1* , Jonathan Brassac1, Benjamin Kilian1,2 and Frank R. Blattner1,3 Abstract Background: Triticeae, the tribe of wheat grasses, harbours the cereals barley, rye and wheat and their wild relatives. Although economically important, relationships within the tribe are still not understood. We analysed the phylogeny of chloroplast lineages among nearly all monogenomic Triticeae taxa and polyploid wheat species aiming at a deeper understanding of the tribe’s evolution. We used on- and off-target reads of a target-enrichment experiment followed by Illumina sequencing. Results: The read data was used to assemble the plastid locus ndhF for 194 individuals and the whole chloroplast genome for 183 individuals, representing 53 Triticeae species and 15 genera. We conducted Bayesian and multispecies coalescent analyses to infer relationshipsandestimatedivergencetimesofthetaxa. We present the most comprehensive dated Triticeae chloroplast phylogeny and review previous hypotheses in the framework of our results. Monophyly of Triticeae chloroplasts could not be confirmed, as either Bromus or Psathyrostachys captured a chloroplast from a lineage closely related to a Bromus-Triticeae ancestor. The most recent common ancestor of Triticeae occurred approximately between ten and 19 million years ago. Conclusions: The comparison of the chloroplast phylogeny with available nuclear data in several cases revealed incongruences indicating past hybridizations. Recent events of chloroplast capture were detected as individuals grouped apart from con-specific accessions in otherwise monopyhletic groups. Keywords: Hybridization, Whole chloroplast genome, Phylogeny, Next-generation sequencing, Divergence times, Triticeae, Wheat, Triticum, Aegilops, Psathyrostachys Background mode of evolution within Triticeae.
    [Show full text]
  • European Red List of Vascular Plants Melanie Bilz, Shelagh P
    European Red List of Vascular Plants Melanie Bilz, Shelagh P. Kell, Nigel Maxted and Richard V. Lansdown European Red List of Vascular Plants Melanie Bilz, Shelagh P. Kell, Nigel Maxted and Richard V. Lansdown IUCN Global Species Programme IUCN Regional Office for Europe IUCN Species Survival Commission Published by the European Commission This publication has been prepared by IUCN (International Union for Conservation of Nature). The designation of geographical entities in this book, and the presentation of the material, do not imply the expression of any opinion whatsoever on the part of the European Commission or IUCN concerning the legal status of any country, territory, or area, or of its authorities, or concerning the delimitation of its frontiers or boundaries. The views expressed in this publication do not necessarily reflect those of the European Commission or IUCN. Citation: Bilz, M., Kell, S.P., Maxted, N. and Lansdown, R.V. 2011. European Red List of Vascular Plants. Luxembourg: Publications Office of the European Union. Design and layout by: Tasamim Design - www.tasamim.net Printed by: The Colchester Print Group, United Kingdom Picture credits on cover page: Narcissus nevadensis is endemic to Spain where it has a very restricted distribution. The species is listed as Endangered and is threatened by modifications to watercourses and overgrazing. © Juan Enrique Gómez. All photographs used in this publication remain the property of the original copyright holder (see individual captions for details). Photographs should not be reproduced or used in other contexts without written permission from the copyright holder. Available from: Luxembourg: Publications Office of the European Union, http://bookshop.europa.eu IUCN Publications Services, www.iucn.org/publications A catalogue of IUCN publications is also available.
    [Show full text]
  • Potential of Wild Relatives of Wheat: Ideal Genetic Resources for Future Breeding Programs
    agronomy Review Potential of Wild Relatives of Wheat: Ideal Genetic Resources for Future Breeding Programs Alireza Pour-Aboughadareh 1,* , Farzad Kianersi 2, Peter Poczai 3,* and Hoda Moradkhani 4 1 Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 3183964653, Iran 2 Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan 65178-38695, Iran; [email protected] 3 Botany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, FI-00014 Helsinki, Finland 4 Department of Agronomy and Plant Breeding, Agricultural College, University of Tehran, Karaj 31587-77871, Iran; [email protected] * Correspondence: [email protected] (A.P.-A.); peter.poczai@helsinki.fi (P.P.) Abstract: Among cereal crops, wheat has been identified as a major source for human food consump- tion. Wheat breeders require access to new genetic diversity resources to satisfy the demands of a growing human population for more food with a high quality that can be produced in variable envi- ronmental conditions. The close relatives of domesticated wheats represent an ideal gene pool for the use of breeders. The genera Aegilops and Triticum are known as the main gene pool of domesticated wheat, including numerous species with different and interesting genomic constitutions. According to the literature, each wild relative harbors useful alleles which can induce resistance to various environmental stresses. Furthermore, progress in genetic and biotechnology sciences has provided accurate information regarding the phylogenetic relationships among species, which consequently opened avenues to reconsider the potential of each wild relative and to provide a context for how we can employ them in future breeding programs.
    [Show full text]