30 JUNE - 3 JULY 2019 Reliable Mycobacteria Diagnostics General Information

30 JUNE - 3 JULY 2019 Reliable Mycobacteria Diagnostics General information

NEW: FluoroType® MTBDR – The 1st LiquidArray • Novel technology: The test system is based on the innovative LiquidArray technology. Venue * Social Media It thus enables reliable MDR-TB diagnostics within 2.5 hours only. The Westin Valencia Twitter: #ESMVal19 • True MDR-TB testing: Resistances to both first-line drugs, rifampicin and isoniazid, are reliably identified, as well as mono-resistances. Amadeo de Saboya, 16 Facebook: @ESMyco • Reliable results: The assay can detect silent mutations within the rpoB gene and 46010 Valencia therefore allows confidence when testing for resistance. +34 963 62 59 00 Delegate list and abstracts Useful venue facts Electronic copies of the delegate list and the abstracts are available in ‘My Mycobacteria Product Series: Rapid, easy-to-use and cost-efﬁ cient! • 500m from metro station Aragon * Congress Materials’ in your ESM Perfect for TB screening FluoroType® MTB • Direct line from airport (metro online account.

Perfect for MDR- / XDR-TB screening FluoroType® MTBDR, GenoType MTBDRplus / sl Alameda * - 25 minutes) Evaluation and certificate Perfect for NTM differentiation GenoType CMdirect, GenoType Mycobacterium CM / AS, • Direct line from North Station GenoType NTM-DR (metro Xativa * – 5 minutes) of attendance • 1 change from Joaquin Sorolla station We hope you enjoy the ESM annual (15 minutes) congress. We really value your feedback and would be grateful if you could www.hain-lifescience.de Registration and information complete the survey, which you will be desk sent by email after the congress. You will be able to download your certificate of Located at the congress entrance of attendance from your online account the hotel. from 15 July. Congress registration will open at 12:00 on Sunday 30 June and be open every For urgent enquiries day until Wednesday 3 July at 13:00. Please call Gaëlle Jamar, ESM Event DRIVING COMPREHENSIVE AND EFFECTIVE SOLUTIONS IN THE FIGHT TO END TB Wi-Fi access Manager: +44 7766 475379 [email protected] Network: Westin BD Sputum Collection Usuario / login: ESM2019 System BD BBL™ Mycoprep, BD BBL™ BD Epicenter™ Data Acid Fast and Fluorescent Stains Contraseña / password: ESM2019 Management System and BD BBL™ Prepared Media with BD EpiCenter™ TB-eXiST Individual Susceptibility Testing* From specimen collection to Come ﬁ nal result, BD is here to support * See map on inside back cover your M. tuberculosis and other and visit mycobacteria testing needs SIRE and PZA fully BD booth ! automated primary DST BD MAX™ Automated with BD BACTEC™ MGIT™ Molecular System Systems

BD MGIT™ TBcID Identification Test* *Not available for sale in the United States BD BACTEC™ MGIT™ Systems bd.com Products are CE marked in compliance with the European In Vitro Diagnostic Medical Device Directive 98/79/EC. BD, the BD Logo and BD Bactec, BD BBL, BD Epicenter, BD Max, and BD MGIT, are trademarks of Becton, Dickinson and Company. © 2019 BD and its subsidiaries. All rights reserved. XEUR5389-19 ESM 40th Annual Congress 2019 Valencia, Spain

AD. UNION.indd 1 29/05/2019 12:10 Welcome address

biology and their translational potential to We hope you enjoy this meeting. innovative control tools. It is our great pleasure and honour Best regards, Valencia is the third most populous city th in Spain and, as a result of being one of to welcome you to the 40 Annual the largest cities in Europe during the XIV century and a prominent Mediterranean trading route, Valencia today sports a Daniela M. Cirillo (President) Congress of the European Society rich and diverse architectural and cultural landscape. The city is also well known of Mycobacteriology as the birthplace of the paella, which can be enjoyed on one of the city’s many Iñaki Comas (Local) beautiful beaches. We are not only looking forward to a stimulating congress, but also to friendly and casual get-togethers in this beautiful environment. Mireia Coscolla (Local)

Last year saw the first UN General starting to see promising results in the Assembly High-level Meeting on Ending vaccine field. TB. The outcome of the meeting put The ESM is a forum where not only state- the spotlight on the need to speed up of-the-art but also incipient research lines the adoption of the 2030 Agenda for are presented and shared. In order to Sustainable Development by increasing make progress in science, two conditions financial support from countries and need to be fulfilled: the availability of industry, and by intensifying research funds, and the field of research being into new tools for tuberculosis control. attractive to young and talented scientists. ESM is committed to the achievement of ESM has been instrumental in increasing these goals through full alignment with the critical mass of basic and clinical the objectives of the European and global scientists dedicated to this field of TB research agenda, and by enabling research and is committed to increasing technology and knowledge transfer their retention in the field by fuelling their to those countries where tuberculosis scientific interests. represents an alarming public health issue. The ESM 2019 programme will address After years of stagnation, the pipeline emerging issues like biomarkers, whole With special thanks to for promising TB diagnostics is now genome based diagnostics, epidemiology increasingly within reach. We expect that of tuberculosis and non-tuberculosis tests for incipient TB, biomarkers suitable mycobacteria, new treatment regimens for point of care diagnosis, and next- for MDR-TB, and ethical aspects of generation sequencing-based diagnostics whole genome sequencing. At the same for MDR-TB will become available within time, it will cover state-of-the-art topics the next few years. In addition, we are on fundamental host and pathogen

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Programme Programme

Sunday 30 June Sunday 30 June

12:00 Registration 17:30- Opening session and awards 20:00 Chairs: Daniela M Cirillo, Iñaki Comas 14:00- Workshop on host pathogen evolution 17:30-17:40 Welcome from the organiser 16:00 Chairs: Mireia Coscollá, University of Valencia; Iñaki Comas, Biomedicine Institute of Valencia (IBV) Bouke de Jong, Institute of Tropical Medicine, Antwerp 17:40-18:00 Opening words from the President 14:00-14:30 GL01 Daniela M Cirillo, San Raffaele Scientific Institute, Milan Dissecting the genetic and evolutionary determinants of immune response variation in humans 18:00-18:45 GL04: Special Lecture Lluis Quintana Murci, Institut Pasteur, Paris What have we learned from the CRyPTIC consortium? Derrick Crook, University of Oxford 14:30-15:00 GL02 What can we learn from ancient horizontal gene transfer in 18:45-19:15 Presentation of Gertrud Meissner Award M. tuberculosis? by Stefan Nieman, Research Center Borstel FZB Olivier Neyrolles, IPSB, Toulouse 19:15-19:45 Presentation of Gardner Middlebrook Award 15:00-15:30 GL03 by Salman Siddiqi, BD Mycobacterial and host determinants of TB granuloma necrosis Antonio Pagan, University of Cambridge Welcome reception at the Palau Alameda * 20:15 (Paseo Alameda corner with Arquitecto Mora, 2 -) * See map on inside back cover 15:30-16:00 OR01 The impact of PE-PPE secretion on the evolution of the Mycobacterium tuberculosis complex Monday 1 July Louis Ates, Amsterdam UMC, University of Amsterdam 09:00- Biology of the host 16:00- Industry symposium 10:30 Chairs: Pere Joan Cardona, Institut Germans Tries i Pujol, Valencia; 17:00 Alexander Apt, Central Tuberculosis Research Institute, Moscow 16:00-16:30 FT MTBDR VER2.0: LiquidArray technology predicts 09:00-09:30 GL05 resistance to rifampicin and isoniazid with high Non-classical innate and adaptive immunity in host defence against accuracy in clinical specimens Mycobacterium tuberculosis in humans Anzaan Dippenaar, DST-NRF Centre of Excellence for Tom Ottenhoff, Leiden University Medical Centre Biomedical Tuberculosis Research | SAMRC Centre for Tuberculosis Research, Pretoria 09:30-09:50 OR02 Mycobacterium tuberculosis modulates sialylation in the lung with an 16:30-17:00 TB control: issue and role of the diagnostic laboratory impact on disease susceptibility in a tertiary care university hospital Kaori Fonseca, i3S, University of Porto Onya Opota, CHUV centre hospitalier universitaire vaudois 09:50-10:10 OR03 17:00- The genetic architecture of the dynamic immune response to 17:30 Coffee break M. tuberculosis infection in human macrophages Joaquín Sanz, Institute BIFI for Bio-computation and Physics of Complex Systems, University of Zaragoza

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Programme Programme

Monday 1 July Monday 1 July

10:10-10:30 OR04 14:00- Poster session I TNF-alpha antagonists differentially induce TGF-beta1-dependent 15:00 Odd numbered posters (P001, P003, P005, P007 etc) Mycobacterium tuberculosis reactivation in a human, in vitro granuloma model Ainhoa Arbués Arribas, Swiss Tropical and Public Health Institute, Basel 15:00- Transmission 10:30- Coffee break 18:00 Chairs: Conor Meehan, University of Bradford; 11:00 Rana Jajou, National Institute for Pubic Health and the Environment RIVM, Bilthoven 11:00- One Health 12:10 Chairs: Thomas Kohl, Research Center Borstel FZB; 15:00-15:30 GL08 Jesús Gonzalo Asensio, University of Zaragoza Ten years and two thousand genomes: Insights from TB WGS in British Columbia, Canada 11:00-11:30 GL06 Jennifer Gardy, University of British Columbia’s School of Population and Mycobacterium tuberculosis throughout Africa and different hosts Public Health,Vancouver using genomics 15:30-15:55 GL09 Mireia Coscolla, University of Valencia TB precision epidemiology: Prospects and challenges of interpreting genomic data in a low burden country 11:30-11:50 OR05 Anders Norman, Statens Serum Institute, Copenhagen Exploring the role of the SigK regulon in the macrophage response to Mycobacterium bovis infection 15:55-16:10 OR08 Alicia Smyth, University College Dublin A descriptive analysis of tuberculosis meningitis associated mortality Michael Lauzardo, University of Florida 11:50-12:10 OR06 Combining genomics and epidemiology to analyse bi-directional 16:10-16:25 OR09 transmission of Mycobacterium bovis in a multi-host system The frequency and rate of insertions and deletions in Mycobacterium Joseph Crispell, University College Dublin tuberculosis during a single outbreak Maxime Godfroid, Kiel University 12:10- Transmission 13:00 Chairs: Troels Lillebaek, Statens Serum Institut; 16:30-17:00 Coffee break Dario Garcia de Viedma, Gregorio Marañon Hospital, Madrid 17:00-17:25 GL10 12:10-12:40 GL07 Beyond frontiers in TB: Transnational surveillance of transmission Diagnostic challenges that affect interruption of transmission Dario Garcia de Viedma, Hospital General Universitario Gregorio Marañon, Bouke de Jong, Institute of Tropical Medicine, Antwerp Madrid

12:40-13:00 OR07 17:25-17:40 OR10 M. tuberculosis microvariation is common and is associated with Comparison of traditional field epidemiology and whole genome rifampicin resistance and transmission: analysis of three years prospective sequencing to understand tuberculosis transmission in a remote universal sequencing in England circumpolar David Wyllie, University of Oxford Jennifer Guthrie, University of British Columbia, Vancouver 13:00- 14:00 Lunch

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Programme Programme

Monday 1 July Tuesday 2 July

17:40-17:55 OR11 10:05-10:20 OR14 Laboratory implementation of next generation sequencing in Kyrgyzstan to Genome-wide mutational biases fuel transcriptional diversity in the stop transmission of drug resistant tuberculosis in TB hospitals Mycobacterium tuberculosis complexi Vanessa Mohr, Research Center Borstel Alvaro Chiner-Oms, Instituto de Biomedicina de Valencia IBV-CSIC

18:00- Ethics lecture and round table 10:20-10:35 OR15 19.30 Chair: Annelies Van Rie, University of Antwerp Survival of Mycobacterium tuberculosis complex strains in the dormant state under hypoxic conditions is lineage-dependent 18:00-18:20 GL11 Tobias Dallenga, Research Center Borstel Ethical challenges in precision approaches to infectious disease: The case of phylogenetic tuberculosis sequencing? 10:35-10:50 OR16 Eric Juengst, UNC Chapel Hill An ancestral lineage of the Mycobacterium tuberculosis complex discovered near the African Great Lakes, missing link between M. canettii 18:20-19:00 Round table discussion and M. tuberculosis sensu stricto Jennifer Gardy - Annelies Van Ries - Bouke De Jong - Daniela Cirillo - Fernando Jean-Claude Ngabonziza Semuto, Rwanda Biomedical Center, Kigali González-Candelas - Dario Garcia de Viedma 10:50- Coffee break 19:30 Guided tour of Valencia 11.20 Dinner at Convent Carmen * (Plaça del Portal Nou, 6): Depart from the 11:20- Non tuberculosis mycobacteria venue, meet at the registration desk * See map on inside back cover 13:05 Chairs: Leen Rigouts, Institute of Tropical Medicine, Antwerp; Stefan Nieman, Research Center Borstel Tuesday 2 July 11:20-11:45 GL13 NTM epidemiology trends, which direction are we going? 09:00- Biology of the pathogen Troels Lillebaek, Statens Serum Institute, Copenhagen 10:50 Chairs: Igor Mokrousov, Saint Petersburg Pasteur Institute; Louis Ates, Amsterdam UMC 11:45-12:15 GL14 NTM susceptibility test pitfalls and clinical implications 09:00-09:30 GL12 Florian Maurer, Research Center Borstel How IS6110 transposition paved the way to construct the live attenuated TB vaccine MTBVAC? 12:15-12:40 OR17 Jesús Gonzalo Asensio, University of Zaragoza Reconstituting the genus Mycobacterium Conor Meehan, BCCM/ITM Mycobacterial Culture Collection, Institute of 09:30-09:50 OR12 Tropical Medicine, Antwerp Adaptation through diversity: differential regulation of leaderless versus canonical translation in Mycobacterium tuberculosis 12:40-13:05 OR18 Anna Grabowska, London School of Hygiene and Tropical Medicine, London Identifying correlates of protection for a vaccine against Mycobacterium ulcerans infection in a low-dose murine challenge model 09:50-10:05 OR13 Tim Stinear, University of Melbourne Genome-wide functional characterization of non-coding RNAs in Mycobacterium tuberculosis using combinatorial CRISPR 13:05- Lunch Thibault Barbier, Department of Immunology and Infectious Diseases, Harvard 14:00 T.H. Chan School of Public Health, Boston

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Programme Programme

Tuesday 2 July Wednesday 3 July

14:00- Poster session II 09:30- New diagnostics and treatment concepts 15:00 Even numbered posters (P002, P004, P006, P008 etc) 12:35 Chairs: Daniela Cirillo, San Raffaele Scientific Institute, Milan; Vlad Nikolayevskyy, Public Health England 15:00- New diagnostics and treatment concepts 16:30 Chairs: Vlad Nikolayevskyy, Public Health England; 09:30-10:00 GL16 Maria Jesus Garcia, Universidad Autonoma de Madrid WHO guidelines on the path to modernize diagnosis, treatment and care of TB 15:00-15:30 GL15 Ernesto Jaramillo, WHO, Geneva TB diagnostics pipeline - Addressing the missing millions and enabling targeted therapy 10:00-10:20 OR22 Claudia Denkinger, FIND & Center of infectious diseases, University High frequency of bedaquiline resistance in programmatically-treated of Heidelberg patients with unfavourable outcomes in Cape Town, South Africa Brigitta Derendinger, Faculty of Medicine and Health Sciences, 15:30-15:50 OR19 Stellenbosch University High percentage of mixed infections in a high MDR-TB country identified from lung surgery samples 10:20-10:40 OR23 Miguel Angel Moreno Molina, Biomedicine Institute of Valencia - CSIC Anti-tuberculosis activity of selected nitronaphtofuran compounds Noelia Alonso Rodriguez, Faculty of Mathematics and Natural Sciences, 15:50-16:10 OR20 University of Oslo Targeting Mycobacterium tuberculosis with lipophilic efflux inhibitors exploring their dual activity: dissipation of the proton motive force and 10:40-11:00 OR24 efflux inhibition A portable real-time solution for next generation sequencing-based Diana Machado, IHMT, Universidade Nova de Lisboa diagnosis of drug-resistant tuberculosis direct from clinical samples Andrea Cabibbe, San Raffaele Scientific Institute, MIlan 16:10-16:30 OR21 A new blood test to accelerate TB diagnosis 11:00-11:30 Coffee break Marc Rodrigue, BioMérieux 11.30-11:55 GL17 16:30- Coffee break 17:00 Relational sequencing TB (ReSeqTB) knowledge base: advances and future directions Paolo Miotto, San Raffaele Scientific Institute, Milan 17.00- General assembly 18:00 11.55-12:15 OR25 Buses depart to Albufera for tour of Albufera and dinner TB portals program: a multi-faceted global response to DR-TB threat. Eric Engle, National Institutes of Health, Bethesda, MD 18:30 Depart from the venue, meet at the registration desk 12:15-12:35 OR26 Whole genome sequencing to distinguish de-novo emergence or secondary infection of multi-drug resistant tuberculosis Vijay Srinivasan

12:35 Poster awards & closing remarks

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Partners Partners

Platinum Silver

Cepheid is a leading molecular diagnostics company that is dedicated FISABIO Sequencing Service provides complete NGS solutions to our to improving healthcare by developing, manufacturing, and marketing customers beginning with a free consultation with our experienced scientists accurate yet easy-to-use molecular systems and tests. By automating highly regarding project design and initiation. We offer both standard and custom complex and time-consuming manual procedures, the company’s solutions solutions for NGS library preparation, sequencing, and bioinformatics deliver a better way for institutions of any size to perform sophisticated analysis support. Our experienced team is available to advise and help with genetic testing for organisms and genetic-based diseases. Through its your project at every step. We provide you with NGS services and capacities strong molecular biology capabilities, the company is focusing on those based on the market leading technologies: applications where accurate, rapid, and actionable test results are needed Illumina Miseq; Illumine NextSeq500; MinION Oxford Nanopore; Sequel most, in fields such as critical and healthcare-associated infections, sexual PacBIO (Soon, end of 2019) health, genetic diseases, virology and cancer. We are involved since 2014 in several Mycobaterium sequencing (Whole www.cepheid.com genome sequencing and RNA-seq) and bioinformatics analysis projects: Swiss tropical and public health institute; Universidade do Minho; Hain Lifescience GmbH is a manufacturer and distributor of in vitro Universidad de Zaragoza; IBV-CSIC (Biomedicine Institute of Valencia); diagnostics based in Nehren in the district of Tübingen. Our customers are Universidad de Valencia; (ITM) The Institute of Tropical Medicine, Belgium; medical laboratories worldwide. We are specialists in molecular genetic test National Centre for TB and Lung Diseases of Georgia; Pasteur Institute systems and technical instruments for the detection of dangerous pathogens (Paris); GSK Global Health R&D Tuberculosis Unit or hereditary diseases. The company was originally founded by David and http://fisabio.san.gva.es/en/fisabio Tobias Hain in 1986 as a mail order company offering laboratory supplies. Over the last 30 years the company developed steadily and successfully. Our QIAGEN is the leading global provider of Sample to Insight® overriding goal is to provide our customers in Germany and everywhere in solutions to transform biological materials into valuable molecular the world with the best possible support. insights. QuantiFERON®-TB Gold Plus (QFT®-Plus) is the only TB test that www.hain-lifescience.de offers >95% sensitivity, the highest specificity of any test for TB infection and innovative CD8+ T-cell technology. Silver www.QuantiFERON.com

BD is one of the largest global medical technology companies in the world Bronze and is advancing the world of health by improving medical discovery, diagnostics and the delivery of care. The company develops innovative Oxford Nanopore Technologies has developed the world’s first nanopore technology, services and solutions that help advance both clinical therapy DNA and RNA sequencing devices. The MinION is a portable, real-time, for patients and clinical process for health care providers. BD and its long-read, low-cost device designed to bring easy biological analyses to 65,000 employees have a passion and commitment to help improve anyone, whether in scientific research, education or real world applications patient outcomes, improve the safety and efficiency of clinicians’ care such as disease/pathogen surveillance, environmental monitoring, food delivery process, enable laboratory scientists to accurately detect disease chain surveillance, self-quantification or microgravity biology. The GridION and advance researchers’ capabilities to develop the next generation of and PromethION devices serve users with larger projects or more samples. diagnostics and therapeutics. BD has a presence in virtually every country Oxford Nanopore Technologies products are currently for research and partners with organizations around the world to address some of use only. the most challenging global health issues. BD helps customers enhance outcomes, lower costs, increase efficiencies, improve safety and expand www.nanoporetech.com access to health care. www.bd.com

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Westin ground floor: Exhibitors

1 7

2 8

4 9

5 10

ESM 40th Annual Congress 2019 Valencia, Spain Partners Partners

Exhibitors Exhibitors

Booth 9 Booth 6 BD A leading pioneer in sequencing, genotyping and bioinformatics since 2001, GenoScreen develops innovation programs that fuel its technological lead and drive the development of innovative services and products. Booth 7 Pioneering Diagnostics With GenoScreen, discover: A world leader in the field of in vitro diagnostics for over 55 years, • Cutting-edge services in genomics, metagenomics and bioinformatics bioMérieux is present in 43 countries and serves more than 160 countries GenoScreen offers a wide range of solutions based on more than 18 years with the support of a large network of distributors. In 2018, revenues of experience and on a technological platform benefiting from the most reached €2.4 billion, with over 90% of international sales. powerful equipment. bioMérieux provides diagnostic solutions (systems, reagents, software, • A key player in molecular microbiology research and innovation services) which determine the source of disease and contamination to Involved in a wide range of research programs and projects, both public improve patient health and ensure consumer safety. Its products are and private, GenoScreen develops a portfolio of analysis and control tools mainly used for diagnosing infectious diseases. Its diagnostic solutions are in genomics, metagenomics and molecular microbiology. also used for detecting microorganisms in agri-food, pharmaceutical and • A company at the service of Humankind and its environment cosmetic products. The services and innovations developed by GenoScreen aim to meet the bioMérieux is listed on the Euronext Paris stock market. needs of academic and industrial researchers facing various problems in www.biomerieux.com the health care of humans, animals, plants and the environment sectors. www.genoscreen.com

Booth 8 Cepheid Booth 2 Hain

Booth 4 With a rightful reputation for innovation, COPAN is the world leading Booth 5 manufacturer of collection and transport systems. In like manner, COPAN’s Insmed is dedicated to improving the lives of patients battling serious and collaborative approach to pre-analytics has resulted in the development rare diseases. Our mission is to develop novel, transformational therapies of FLOQSwabs®, ESwab®, UTM® and modular laboratory automation, that make a real difference to patients. WASP® and WASPLab®. www.insmed.com www.copangroup.com Booth 1 Booth 10 Qiagen Fisabio

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Poster session 1: Monday 1 July Poster session 1: Monday 1 July

P011 P023 P033 P045 Genomic characterization of M. tuberculosis closely related variants Ultrastructural comparison by electron “Hole” genome sequencing: Illumina Mycobacterium africanum West-African with different transmission success also microscopy of M. tuberculosis Beijing blind spots in the M. tuberculosis H37Rv 1 (Lineage 5) show different behaviour in an ex vivo and Beijing-like genotypes isolated from genome C. N’Dira Sanoussi, Laboratoire de infection model Colombian patients Faramarz Valafar, San Diego State Référence des Mycobactéries, Cotonou, Estefania Abascal, Hospital General Martha Isabel Murcia Aranguren, University Benin Universitario Gregorio Marañón, Madrid Universidad Nacional de Colombia BIOLOGY OF THE PATHOGEN BIOLOGY OF THE PATHOGEN BIOLOGY OF THE PATHOGEN P047 P013 P025 P035 Towards next generation diagnostics NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT Uncovering the epitopes underlying the Genetic diversity of Mycobacterium Genome-based taxonomic workflow for tuberculosis: identification of induction of varying adaptive immune tuberculosis Beijing strains in China for defining new species within the novel molecular targets by large-scale responses by different Mycobacterium Yang Zhou, Chinese Center for Disease Mycobacterium genus comparative genomics tuberculosis lineages Control and Prevention Sari Cogneau, Institute of Tropical Galo Goig, Institute of Biomedicine of Carlos Magalhães, University of Minho, Medicine Antwerp Valencia Braga P027 P037 P049 P015 Primary multidrug-resistance and genotypic characterization of Comparison of drug susceptibility Discordances between molecular Exploring mechanisms of translation in Mycobacterium tuberculosis strains testing performed on TB specimens assays for detection of rifampicin the human pathogen, Mycobacterium circulating in North-West Russia from HIV patients in a TB high-incidence resistance in Mycobacterium tuberculosis Anna Vyazovaya, St. Petersburg Pasteur country, locally and in a supranational tuberculosis: frequency, mechanisms Beth Sawyer, London School of Hygiene & Institute reference laboratory and clinical impact Tropical Medicine Dorte Bek Folkvardsen, Statens Serum Michael Whitfield, University of Antwerp P029 Institut, Copenhagen P017 P051 Characterization of the P039 Ethambutol resistance: An interplay intramacrophagic expression profile of Performance of the Deeplex®-MycTB of efflux genes overexpression and the smallRNA ncRv0757c in different Homopolymers in Mycobacterium kit on high quality DNA samples and genomic mutations Mycobacterium tuberculosis lineages tuberculosis: A source for rapid on clinical samples for the detection of Mandira Varma-Basil, University of Delhi Matteo Chiacchiaretta, IRCCS Ospedale adaptation? first- and second-line drug resistance in San Raffaele, Milano Michaela Zwyer, University of Basel MTBC strains P019 Silke Feuerriegel, Research Centre Borstel P031 P041 Mechanisms of drug tolerance in P053 Mycobacterium tuberculosis Genotypes and drug resistance Gene panel for MTBC drug resistance Annelies Van Rie, University of Antwerp of Mycobacterium tuberculosis detection by using next generation CAPTURE-XT -Isolation and from patients with HIV-associated sequencing technologies concentration of Mycobacterium P021 tuberculosis in St. Petersburg, Russia Carla Mariner Llicer, Biomedicine institute tuberculosis from sputum using a novel Alena Gerasimova, St. Petersburg Pasteur of Valencia (IBV-CSIC) The role of Mycobacterium tuberculosis microfluidic-dielectrophoretic (DEP) Institute technology to enable rapid diagnosis complex genetic variation and of P043 macrophage phenotype during early Laura Katvars, QuantuMDx, Newcastle stages of infection Emergence of bedaquiline resistance in NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT Paolo Miotto, IRCCS Ospedale San M. tuberculosis Raffaele, Milano Danila Zimenkov, Russian Academy of Sciences, Moscow

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Poster session 1: Monday 1 July Poster session 1: Monday 1 July

P055 P067 P077 P087 Missed ethionamide resistance in Development of a tuberculosis Whole genome sequencing analysis to In vitro activity of Linezolid and Tedizolid South Africa diagnostic kit based on next generation identify SNPs involved in bedaquiline against Mycobacterium tuberculosis Marisa Klopper, Stellenbosch University, sequencing and delamanid phenotypic resistance in Pilar Ruiz-Martinez, University of Córdoba Cape Town Maria Piñana, Hospital Universitari Vall M. tuberculosis clinical strains d’Hebron (HUVH), Barcelona Simone Battaglia, San Raffaele Scientific P089 P057 Institute, Milan P069 New generation Interferon-Gamma Isoniazid resistance determination: P079 Release Assay (QFT-PLUS): a possible challenges in practice Fluoroquinolone (hetero)resistant tool to evaluate the response to NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT Elizabeth Streicher, Stellenbosch TB assessed by deep sequencing: a Evaluation of GeneXpert MTB/RIF treatment in active tuberculosis University, Cape Town nationwide study for detection of Mycobacterium Paola Dal Monte, University of Bologna Philip Supply, Univ. Lille, CNRS, Inserm, tuberculosis complex and rifampicin P059 CHU Lille, Institut Pasteur de Lille, U1019 - resistance in non-respiratory clinical P091 UMR 8204 - CIIL Rapid detection of mycobacterial specimens at TB Lab in Rashid Hospital Can we trust PZA DST? An investigation growth by fluorogenic media which can in Dubai of EQA results from 7 NRLs and 13 P071 Maya Habous, Dubai Health Authority be used for rapid susceptibility testing clinical TB-laboratories Tanil Kocagoz, Acibadem Mehmet Ali Mycobacterial species identification P081 Melles Haile, Public Health Agency of Aydinlar University, Istanbul from grown Middlebrook broth with Sweden, Solna VITEK®MS MaldiTof Evaluation of cyclopiazonic acid activity P061 Ana Gil-Brusola, Hospital Universitario y on Mycobacterium tuberculosis CtpF: P093 Politécnico La Fe, Valencia High-dose isoniazid continues to potential target for anti-tubercular drugs Management of hazardous infectious Santos Ruiz, Universidad Nacional de contribute to treatment success in the P073 waste in a Mycobacteria reference majority of MDR-TB patients Colombia center Pauline Lempens, Institute of Tropical Identification of a highly successful P083 Esther Vaquero-Alvarez, University of Medicine, Antwerp MDR-TB clone transmitting in the high Cordoba burden setting of Callao, Peru Transcriptional profiling of P063 Christian Utpatel, Research Centre Borstel Mycobacterium tuberculosis suggests P095 respiratory poisoning upon exposure to Unveiling the synergistic mechanisms P075 Application of superparamagnetic of M. tuberculosis and HIV-1 through delamanid nanoparticles for fast, simultaneous and An Van den Bossche, Sciensano, Brussels SPINET, a protein-protein interaction Evaluation of the Xpert MTB/RIF Ultra non-invasive diagnosis of tuberculosis network analysis tool (XTB-U) assay for Direct Detection of P085 and HIV infection Ana Santos-Pereira, University of Minho, Mycobacterium tuberculosis (MTC) in Diana Machado, Universidade Nova de Braga selected respiratory specimens with Mycobacterium tuberculosis isolates Lisboa minimal bacillary load subcultured on different growth media P065 Miguel José Martínez-Lirola, Complejo show no evidence of passage artifacts P097 Hospitalario Universitario Torrecárdenas, Identifying markers for high-dose INH and remain representative of original How well do routine molecular Almería treatment clinical samples diagnostics detect rifampicin Annelies Van Rie, University of Antwerp Charlotte Genestet, CIRI, INSERM U1111, heteroresistance in Mycobacterium Université de Lyon tuberculosis? Kamela Ng, Institute of Tropical Medicine, Antwerp

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Poster session 1: Monday 1 July Poster session 1: Monday 1 July

P099 P111 P121 P133 Whole-genome analysis of Perception for risk and disease severity A phygenomic perspective on Expanded trans-national tracking of a

Mycobacterium chimaera as a of NTM lung disease- physician survey ONE HEALTH a neglected infectious disease: Beijing strain successfully transmitted potentially useful tool for description of in Germany, UK, Italy, France and the tuberculosis by Mycobacterium bovis in in France outbreak associated to heater–cooler Netherlands North Brazil Laura Perez-Lago, Hospital General units (HCUs) Roald van der Laan, Insmed Europe Marília Lima da Conceição, PPGBPA, Universitario Gregorio Marañón, Madrid Francesco Messina, IRCCS, Rome UEPA, Lisbon P113 P135 P101 P123 Safety and toxicity study of TRANSMISSION OF TUBERCULOSIS Characterizing a TB outbreak in a prison NON TUBERCULOSIS MYCOBACTERIA NON TUBERCULOSIS MYCOBACTERIA Understanding the role and regulation the immunomodulatory agent Multidrug resistant tuberculosis in Spain in Costa Rica following non-standard of efflux pumps expression in an in-vitro Mycobacterium brumae in two different Sofia Samper, Universidad de Zaragoza laboratory approaches evolved drug resistance Mycobacterium animal models Laura Perez-Lago, Hospital General Deepika Rai, Indian Institute of Technology, Esther Julian, Universitat Autònoma de P125 Universitario Gregorio Marañón, Madrid Bombay Barcelona Population structure of Mycobacterium P137 P103 P115 bovis in Germany: a long-term study using whole genome sequencing WGS reveals a likely long-term prevalent Epidemiological typing of Performance of the GenoType CMdirect combined with conventional molecular status for a predominant Beijing strain Mycobacterium xenopi by multilocus VER 1.0 assay on formalin fixed paraffin typing methods in Colon, Panama sequence typing embedded (FFPE) biopsies: three case Thomas Kohl, Research Center Borstel Fermin Acosta, Instituto de Investigación Maria Teresa Tórtola Fernández, reports Sanitaria Gregorio Marañón, Madrid Universitary Vall d’Hebron. UAB.Barcelona Anna Camaggi, Maggiore della Carità P127 University Hospital, Novara P139 P105 Proposal for control of ventilation P117 with low-cost sensors that use Phylogeny of modern Beijing clusters M. In vitro susceptibility of nontuberculous a standardized and open data tuberculosis in Mongolia Mycobacterium (NTM) isolates to Emerging source of infection – management model based on the Svetlana Zhdanova, Scientific Center of

Tedizolid (TZD) ONE HEALTH M. tuberculosis in a rescue dog, European FiWare standard Family Health and Human Reproduction, Jenny Wee, Singapore General Hospital a case report Esther Vaquero-Alvarez, University of Irkutsk Silja Mentula, National Institute for Health Cordoba P107 and Welfare, Helsinki P141 P129 Use of whole genome sequencing for P119 Dynamic changes in MDR M. investigating iatrogenic infections due Evidence for Pleistocene zoonotic tuberculosis in northwestern Russia: an to non-tuberculous mycobacteria Field appication of antigen pre- tuberculosis origins update from Russian-EU border region Emmanuel Lecorche, National coated blood collection tubes for the David Minnikin, University of Birmingham Igor Mokrousov, St. Petersburg Pasteur Reference Center for Mycobacteria and IFN-r assay for diagnosis of bovine Institute Antimycobacterial Resistance, Paris tuberculosis P131 Yun-Ho Jang, Animal and Plant quarantine P143 Fast molecular approach for scrutiny P109 agency, Korea of TB recurrences involving epidemic High rates of drug resistance and Mycobacterium pseudoshottsii in Italian multidrug-resistant strains of emerging M. tuberculosis clones in marine water fish farms Mycobacterium tuberculosis a specialised penitentiary facility in Davide Mugetti, Istituto Zooprofilattico Laura Perez-Lago, Hospital General eastern Ukraine Sperimentale del Piemonte, Liguria e Valle Universitario Gregorio Marañón, Madrid Vladyslav Nikolayevskyy, Public Health d’Aosta – Torino England, Imperial College London TRANSMISSION OF TUBERCULOSIS

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Poster session 1: Monday 1 July Poster session 2: Tuesday 2 July

P145 P155 P002 P012 Genomic survey in Liberia reveals Contribution of whole genome Mycobacterium tuberculosis associated Will the real pan-genome please high burden of MDR-TB and multiple sequencing for the detection of with severe tuberculosis evades stand up? Investigating the protein- importation events tuberculosis transmission in healthcare cytosolic surveillance systems to coding regions of the Mycobacterium Mariana Gabriela López, Instituto de settings: the experience of a french modulate IL-1β production tuberculosis complex

Biomedicina de Valencia center BIOLOGY OF THE HOST Jeremy Sousa, i3S, University of Porto Christopher Beaudoin, University of Oana Dumitrescu, University Hospital of Cambridge BIOLOGY OF THE PATHOGEN P147 Lyon P004

TRANSMISSION OF TUBERCULOSIS TRANSMISSION OF TUBERCULOSIS P014 Differences in tuberculosis transmission P157 The extraordinary impact of dynamics between two adjoining Mycobacterium tuberculosis in the Cryo-EM beauty and benefit for settings applying or not multidisciplinary Impact of Mycobacterium tuberculosis Paleolithic predicts unprecedented drug discovery and vaccine design: intervention oriented by molecular complex lineages as a determinant of population growth due to female our achievements with T7SS in epidemiology disease phenotypes from an immigrant protection against tuberculosis Mycobacterium tuberculosis Miguel José Martínez-Lirola, Complejo rich moderate tuberculosis burden Pere-Joan Cardona, Universitat Autònoma Peter J. Peters, Maastricht University Hospitalario Universitario Torrecárdenas, country de Barcelona, CIBERES. Badalona Medical Center Almería Sahal Al-Hojoj Al-Nakhli, King Faisal Specilaist Hospital and Research Center, P006 P016 P149 Saudi Arabia Ex vivo characterization of CD56neg Pinpointing genetic intra-host diversity Elucidating the value of whole-genome CD16pos NK cell subpopulation in of Mycobacterium tuberculosis sequencing for a rapidly spread active tuberculosis and latently infected Charlotte Genestet, CIRI, INSERM U1111, outbreak analysis subjects Université de Lyon Sofia Samper, Instituto Aragones de Nurhan Albayrak, Université Libre de Ciencias de la Salud Bruxelles (ULB) P018 P151 P008 Identification of putative compensatory mutations in rpoA/C suggests Bridging WGS data with classical Insights into lung microbiome dysbiosis contribution to the fixation of RIF genotyping Mycobacterium tuberculosis in patients with active pulmonary resistance in M. tuberculosis profiles tuberculosis Faramarz Valafar, San Diego State Guislaine Refrégier, I2BC, CNRS-CEA, Monica Ticlla, Swiss Tropical and Public University Université Paris-Saclay Health Institute, Basel P020 P010 System multi-omics analysis of The lipid environment modulates the Mycobacterium tuberculosis Beijing B0/ stress response of Mycobacterium W148 cluster tuberculosis Egor Shitikov, Federal Medical Biological Maria Jesus Garcia, Autonoma University Agency, Moscow of Madrid BIOLOGY OF THE PATHOGEN

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Poster session 2: Tuesday 2 July Poster session 2: Tuesday 2 July

P022 P034 P044 P054 An updated functional annotation of Emergence of genetic variants in Strong association of mutations known Mycobacterium smegmatis as a Mycobacterium tuberculosis reference longitudinal M. tuberculosis isolates to confer resistance to three injectable model organism for Mycobacterium strain H37Rv associated with increased minimum drugs in a multinational analysis tuberculosis: are fast and slow that Faramarz Valafar inhibitory concentrations of ethambutol Faramarz Valafar, San Diego State similar? San Diego State University Ha Vu Thi Ngoc, Oxford University Clinical University F T Subtil, The Francis Crick Institute Research Unit & Ho Chi Minh City, BIOLOGY OF THE PATHOGEN BIOLOGY OF THE PATHOGEN P024 Vietnam P046 P056 Resuscitation of BCG in blood samples LL37 derivated peptides containing Critical pulmonary tuberculosis: P036 CONCEPTS NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT of BCG vaccinated individuals unnatural D amino acids and peptoid Identifying candidate molecular markers Stefan Panaiotov, National Center Insights on Mycobacterium leprae efflux present inhibition Na+/K+, Cu+ and for diagnostic, prognostic and virulence. of Infectious and Parasitic Diseases, pumps and their implications in drug Zn+2 ATPases in mycobacterial plasma Gonzalo Greif, Instituto Pasteur Sofia resistance and virulence membrane and synergy in combination Montevideo, Montevideo Diana Machado, Universidade Nova de with isoniazid and kanamycin P026 Lisboa Sandra Chingaté López, Universidad P058 Nacional de Colombia Predicting Mycobacterium tuberculosis P038 First evaluation in routine use of the population structure by MIRU-VNTR P048 combination of GeneLeadVIII to extract Yang Zhou, Chinese Center for Disease Monitoring a multidrug-resistant and detect Mycobacterium tuberculosis Control and Prevention Mycobacterium tuberculosis endemic Rifampin-nitazoxanide, but not rifampin- (Mtb) DNA and Deeplex-MycTB genotype in Bulgaria isoniazid-pyrazinamide-ethambutol kills to predict drug resistance and TB P028 Stefan Panaiotov, National Center of dormant Mycobacterium tuberculosis in transmission in less than one week from Infectious and Parasitic Diseases, Sofia Mycobacterium tuberculosis defective caseum mimicking conditions clinical samples Wladimir Sougakoff, National Reference in the heavy metal transporter CtpA as a Lanfranco Fattorini , Istituto Superiore di P040 Centre of Mycobacteria NRC MyRMA, potential attenuation target Sanità, Rome Paris Gina Marcela Lopez Ruiz, Universidad PE_PGRS3 of Mycobacterium P050 Nacional de Colombia tuberculosis is specifically expressed P060 at low phosphate concentration, and Evaluation of the FluoroType® MTBDR P030 its arginine-rich C-terminal domain Version 2.0 assay for improved detection Exploring Bedaquiline resistance- Typing of Mycobacterium tuberculosis mediates adhesion and persistence in and differentiation of rifampicin and associated genetic regions in clinical by whole genome sequencing reveals host tissues isoniazid resistance in Mycobacterium samples from a cohort study of Michela Sali Fondazione Policlinico a novel X3 lineage associated with tuberculosis complex Rifampicin resistant TB patients in Universitario A. Gemelli, IRCCS, Roma multidrug resistance circulating in Anzaan Dippenaar, Stellenbosch University, South Africa southeast Mexico Cape Town Annelies Van Rie, University of P042 Antwerp Roberto Zenteno-Cuevas, Universidad P052 Veracruzana, Veracruz Near-untreatable TB: lessons learned P062 and future directions Discovery of a new class of anti- P032 Marisa Klopper, Stellenbosch University, tubercular antibiotic originating from the Phenotypical and molecular Virulence and immunopathology Cape Town gut microbiota characterization of streptomycin- induced by Mycobacterium tuberculosis Dereje Abate Negatu, National University of resistant M. tuberculosis clinical isolates genotype Beijing and Beijing-like Singapore from a Portuguese cohort isolated in Colombia Deisy Rocha Silva, University of Minho, Martha Isabel Murcia Aranguren, Braga NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT Universidad Nacional de Colombia

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Poster session 2: Tuesday 2 July Poster session 2: Tuesday 2 July

P064 P076 P086 P096 Discordance across molecular How do Nipro line probe assays perform Preliminary in vitro activity of linezolid Evaluation of FluoroType MTBDR used and phenotypic methods for drug on the Hain Twincubator? and tedizolid against Mycobacterium for different specimen types susceptibility testing of Mycobacterium Sarah Sengstake, Institute of Tropical avium complex Erik Svensson, Statens Serum Institut, tuberculosis Medicine, Antwerp Pilar Ruiz - Martinez, Reina Sofía University Copenhagen Nelly Ciobanu, Phthisiopneumology Hospital-IMIBIC, Cordoba Institute, Chisinau, Moldova P078 P098 P088 A pilot study of new external quality P066 Application of nanopore sequencing for drug resistant prediction of Comparison of two mapping algorithms assessment applied to a tuberculosis NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT CONCEPTS NEW DX AND TREATMENT NON TUBERCULOSIS MYCOBACTERIA Evaluation of new molecular test Allplex Mycobacterium tuberculosis complex for lineage and resistance prediction of microscopy laboratory network in MTB/MDRe/XDRe Detection for rapid strains MTBC samples Mexico identification of drug resistance in Francy Johanna Pérez Llanos, Research Margo Diricks, Applied Maths Carolina Flores-Marroquin, Institute Mycobacterium tuberculosis Center Borstel (bioMérieux) of Diagnosis Reference Epidemiology, Eva Sodja, University Clinic Golnik, Mexico Slovenia P080 P090 P100 Introducing a new target for specific Sensitivity evaluation of LIAISON® P068 Genomic analysis of cardiac surgery- detection of Mycobacterium QuantiFERON TB GOLD Plus assay, associated Mycobacterium chimaera Search for novel antituberculosis drug tuberculosis complex using modified a new automated chemiluminescent infections in Northern Italy targets: Biotin biosynthesis pathway genome comparison method immunoassay (CLIA) for diagnosis of Arash Ghodousi, San Raffaele Scientific Mandira Varma-Basil, University of Delhi Reza Kamali Kakhki, Mashhad University of tuberculosis infection Institute, Milan Medical Sciences, Mashhad Paola Mantegani, San Raffaele Scientific P070 Institute, Milan P102 P082 MYCO-TB decontamination kit: P092 Each mycobacterium requires particular evaluation of suitability for molecular Bridging the TB data gap: Extracting NON TUBERCULOSIS MYCOBACTERIA culture conditions to maximize its biology and mycobacteria detection rifampicin-resistance diagnostic test Preliminary comparison between BD immunomodulatory and antitumoral with extended time of incubation probe reactions from Mycobacterium MAX and Xpert MTB/RIF Ultra for effects Giulia Lombardi, S. Orsola-Malpighi tuberculosis whole genome sequence rapid detection of Mycobacterium Sandra Guallar Garrido, Universitat University Hospital - University of data tuberculosis complex (MTB) in Autònoma de Barcelona Bologna Kamela Ng, Institute of Tropical Medicine, pulmonary and extrapulmonary Antwerp specimens P104 P072 Anna Camaggi, University Hospital, Whole genome sequencing (WGS) for P084 Novara Acquisition of cross-resistance to the subspeciation of clinical isolates of Mycobacterium abscessus complex Bedaquiline and Clofazimine following Molecular characterization of mutations P094 treatment for tuberculosis associated with resistant tuberculosis in (MABC) in a clinical laboratory Arash Ghodousi, San Raffaele Scientific Northeast Brazil Anti-tuberculosis treatment initiation Jenny Wee, Singapore General Hospital Institute, Milan Luana Lima, Evandro Chaga Institute, within seven days of respiratory sample Pte Ltd Anarindera, Para collection: the utility of the nucleic acid P106 P074 amplification test (NAAT) compared to TB1 and TB2 positivity rates with acid-fast smear microscopy Challenging features in mycobacteria Marie Nancy Seraphin, University of Florida QuantiFERON-TB Gold Plus Assay identification by MALDI-TOF MS Zeynep Saribas, Hacettepe University, David Rodriguez Temporal, Hospital Ankara Universitari de Bellvitge-IDIBELL, Hospitalet de Llobregat

ESM 40th Annual Congress 2019 Valencia, Spain ESM 40th Annual Congress 2019 Valencia, Spain Poster session 2: Tuesday 2 July Poster session 2: Tuesday 2 July

P108 P122 P136 P150 Invasive Mycobacterium chimaera infection Outbreak of bovine tuberculosis by Integrative transnational analysis to Drug resistance landscape of

after cardiac surgery: a case report ONE HEALTH Mycobacterium bovis in cattle and dissect non-obvious transmission Mycobacterium tuberculosis complex Agnese Denicolo, The Romagna Hub buffalo in Northern Brazil events involving migrants strains in Cameroon after successful Laboratory, Pievestina Karla Lima, Universidade do Estado do Laura Perez-Lago, Hospital General introduction of the short course MDR- Pará, Belém-PA Universitario Gregorio Marañón, Madrid TB regimen P110 Matthias Merker, Research Center P124 Underestimation of the pathogenic role P138 Borstel Insights into the global phylogeography

of Mycobacterium fortuitum and its TRANSMISSION OF TUBERCULOSIS The Emergence of Mycobacterium TRANSMISSION OF TUBERCULOSIS of Mycobacterium bovis P152 NON TUBERCULOSIS MYCOBACTERIA related species in fishes and humans tuberculosis L4.5 in Lebanon shows a Davide Mugetti, Istituto Zooprofilattico Chloé Loiseau, Swiss Tropical and Public Molecular epidemiology of Health Institute, Basel change of tuberculosis transmission Sperimentale del Piemonte, Liguria e Valle dynamics Mycobacterium tuberculosis in urban Tanzania d’Aosta – Torino P126 Khaldoun Masoud, American University of Liliana K. Rutaihwa , Swiss Tropical and Beirut P112 Etiology of mycobacterial infection in Public Health Institute, Basel HIV-infected patients in Ukraine Screening for NTM lung disease in adult P140 Anna Barbova, National Institute P154 non-CF adult bronchiectasis patients - of Phthisiology and Pulmonology, Evolution of Mycobacterium physician survey in Germany, UK, Italy, Whole genome sequencing provides tuberculosis complex strains during France and the Netherlands Ukraine additional insights into recurrent longitudinal transmission in a high drug Roald van der Laan, Universitätsklinikum P128 tuberculosis classified as endogenous Freiburg resistant setting reactivation by IS6110 DNA Direct DNA modified CTAB preparation Ivan Barilar, Research Center Borstel fingerprinting P114 from nasal exudate in live M. bovis Anzaan Dippenaar, Stellenbosch University, infected cattle in Mexico provide with a P142 Tentative epidemiological cutoff Cape Town valuable routine assay extrapolated to values for Mycobacterium kansasii, Genotypic study using the MIRU- humans TB diagnostic test P156 Mycobacterium xenopi and VNTR technique of clinical isolates Gloria Guerrero Manriquez, Universidad Mycobacterium marinum of Mycobacterium tuberculosis in a Mycobacterium tuberculosis complex Autonoma de Zacatecas Florian Maurer, Research Center Borstel cohort of patients with drug-resistant lineage 3 strains drive a pulmonary P130 tuberculosis in Colombia 2012-2013 tuberculosis outbreak in Eastern Sudan P116 Lorena Alexandra & Argoty Chamorro, Yassir Shuaib, Research Center Borstel Towards genotype resolution of mixed Evaluation of the Colour Test in Universidad Nacional de Colombia Mycobacterium tuberculosis infections P158 ONE HEALTH inexperienced settings in WGS analysis P146 Kadri Klaos, Tartu University Hospital Pedro Sola-Campoy, Hospital General Genetic Lineages of Mycobacterium P118 Universitario Gregorio Marañón, Madrid Different historical patterns underlie tuberculosis Display Distinctive the M. tuberculosis Lineage 4 currently Demographic and Migration Evaluation of 8 VNTR loci and P132 circulating in Vietnam and Indonesia Characteristics in Hawaii spoligotyping for molecular Unexpected findings of tuberculosis by Philip Ashton, Oxford University Clinical James Douglas, Affiliation University of characterisation of Mycobacterium TRANSMISSION OF TUBERCULOSIS the GeneXpert MTB/RIF method – case Research Unit & Ho Chi Minh City, Vietnam Hawai bovis and Mycobacterium caprae reports isolates from cattle in Bulgaria Maria Mullerova, CITYLAB, Prague P148 Violeta Valcheva, Bulgarian Academy of ECDC pilot study on the use of whole Sciences, Sofia P134 genome sequencing for MDR-TB P120 Testing whole genome sequencing as surveillance in Europe: results of the gold standard to improve transmission first year of the project implementation The epidemiological situation of bovine tracing: a retrospective study in a low- Elisa Tagliani, San Raffaele Scientific tuberculosis in Korea burden region Institute, Milan Yun-Ho Jang, Animal and Plant quarantine Irving Cancino, Biomedicine Institute of agency, Korea Valencia ESM 40th Annual Congress 2019 Valencia, Spain Key locations 6

The Westin Valencia

Palau Alameda

4 1 3 3 2 Aragon Metro

Alameda Metro

5 Colon Metro

Convent Carmen 7 7

Xátiva Metro EUROPEAN SOCIETY OF MYCOBACTERIOLOGY 41ST ANNUAL CONGRESS 28 JUNE - 1 JULY 2020

ROGNER HOTEL | TIRANA, ALBANIA PRESIDENT OF ESM: DANIELA CIRILLO | PRESIDENT OF THE LOC: SILVA TAFAJ