Technical Data Sheet

Te c h n i c a l Da t a Sh e e t

1,1’,2,2’ Tetraoleoyl Cardiolipin (sodium salt) Catalog Number 710335 Physical state Powder; chloroform solution Purity > 99% Transition temp. No data CAS 115404-77-8 CMC No data Synonyms 18:1 CA PKA No data

Molec. Formula C81H148O17P2Na2 TLC mobile phase C:M:W & C:M:A*, 65:25:4, v/v; C:Hexane:M:Acetic Acid 50:30:10:5, v/v, if want to see PG MW 1,501.000 Exact Mass 1,501.000 Percent composition C 64.77% H 9.93% O 18.11% P 4.12% Na 3.06% Stability Store in <-20°C freezer for 6 months Solubility Soluble in chloroform and alcohol (warmed with water content 3-5%) at concentrations up to 10 mg/mL. Insoluble in water and acetone. Web link 710335 * chloroform:methanol:water and chloroform:methanol:acetone

Description: Cardiolipin, the mitochondrial-specific phospholipid, helps maintain mitochondrial function, membrane potential and structural support for the inner mitochondrial membrane and the proteins in it (Chicco and Sparagna, 2007). Concentration and composition changes of cardiolipin have been implicated in pathological conditions including ischemia, hypothyroidism, aging, heart failure and cardioskeletal myopathy (Barth syndrome) (Chicco and Sparagna, 2007). Cardiolipin has also been shown as a signaling molecule in apoptosis (Kagan et al, 2006; Ritov et al, 2006). Product use: Soluble in chloroform and alcohol (warmed with water content 3-5%) at concentrations up to 10 mg/mL. Insoluble in water and acetone. Also used in liposomes. Please use the following web link for liposome preparation. References: • Huang and Frohman (2009). Lipid Signaling on the Mitochondrial Surface. Biochim Biophys Acta. 2009 Jun 17. [Epub ahead of print]. • Chicco and Sparagna (2007). Role of cardiolipin alterations in mitochondrial dysfunction and disease. Am J Physiol, Cell Physiol 292(1): C33-44 • Matsumo et al (2006). Lipid domains in bacterial membranes. Mol Microbiol 61(5): 1110-7 • Kagan et al (2006). The “pro-apoptotic genies” get out of mitochondria: oxidative lipidomics and redox activity of cytochrome c/cardiolipin complexes. Chem Biol Interact 163(1-2): 15-28 • Ritov et al (2006). Analysis of cardiolipin in human muscle biopsy. J Chromatogr B 831:63-71