g16482.t1@[email protected] Page 1 of 1

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100 Candidatus Thiomargarita nelsonii KHD07353 Bacillus thuringiensis Bt407 YP_006926419 Legionella pneumophila WP_014842957 88 Endozoicomonas montiporae WP_034879478 Acinetobacter baumannii WP_031968362 100 87 Vibrio nigripulchritudo WP_004402447 98 Psychrobacter sp. P11G3 WP_057760119 100 99 Xenorhabdus bovienii WP_038203911 100 Rouxiella chamberiensis WP_045046329 18 Dendroctonus ponderosae ERL83390 Serratia sp. M24T3 WP_009636677 98 56 100Lonsdalea quercina WP_026743324 Escherichia coli str. K-12 substr. MG1655 NP_415996 86 98 Chlorobi bacterium OLB5 KXK50071 CoptotermesTric_AAA92714 formosanus AGM32510 100 Histomonas meleagridis ACI16482 100Eucalyptus grandis XP_010033838 95 98 Ectocarpus siliculosus CBJ25596 Guillardia theta CCMP2712 XP_005838347 Phaeodactylum tricornutum CCAP 1055/1 XP_002180331 99 Aphanomyces astaci XP_009825348 95 100 Albugo laibachii Nc14 CCA15917 100 Phytophthora parasitica P1569 ETI54450 100 Phytophthora infestans T30-4 XP_002896607 100 Brugia malayi CDP93853 Bombus impatiens XP_003487345 Trichoplax adhaerens XP_002116081 95 Apteryx australis mantelli XP_013810665 100 100 Callorhinchus milii XP_007891153 Homo sapiens AAC50613 93 Callorhinchus milii XP_007900551 100 Clupea harengus XP_012674444 99 Chrysochloris asiatica XP_006865016 82 76 100 84Nannospalax galili XP_008842357 98 Cricetulus griseus XP_007626683 100 65Rousettus aegyptiacus XP_016015809 Condylura cristata XP_004683330 58Chinchilla lanigera XP_013367523 85 61Microcebus murinus XP_012594174 63Propithecus coquereli XP_012497307 9821Aotus nancymaae XP_012324900 26Pan troglodytes XP_508682 94Saimiri boliviensis boliviensis XP_003935120 70 Callithrix jacchus XP_002754768 9825Homo sapiens XP_005273774 Nematostella vectensis XP_001625625 Porphyridium purpureum Porpur_536.1 Volvox carteri f. nagariensis XP_002945620 96 Sutterella sp. KLE1602 WP_062331431 Bradyrhizobium sp. Ec3.3 WP_027524465 58 Guillardia theta CCMP2712 XP_005841372 76 Goniomonas sp m CAMPEP_0114548656 100 100 Vitrella brassicaformis CCMP3155 CEM35928 Achromatium sp. WMS3 KOR33113 100 Perkinsus marinus ATCC 50983 XP_002766957 Perkinsus marinus ATCC 50983 XP_002766872 42 Perkinsus marinus ATCC 50983 XP_002780610 100 98 Monosiga brevicollis MX1 XP_001747494 82 Mucor circinelloides f. lusitanicus CBS 277.49 OAD03950 Porphyridium purpureum Porpur_2035b.42 56 100 Mayorella sp BSH-02190019 CAMPEP_0174234610 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168541028 82 Acanthamoeba castellanii str. Neff XP_004368256 94100 Emiliania huxleyi CCMP1516 XP_005760562 Isochrysis aff. galbana Isogal35893_c0_seq6 99 100 Saprolegnia parasitica CBS 223.65 XP_012210381 100 Phytophthora parasitica INRA-310 XP_008891601 Phytophthora infestans T30-4 XP_002901849 97 100 Thalassiosira pseudonana BAO52744 73 Guillardia theta CCMP2712 XP_005831287 100 Goniomonas sp m CAMPEP_0114573766 95 Eutreptiella gymnastica NIES-381 CAMPEP_0113771732 76 Aureococcus anophagefferens Auran_58666 100 Naegleria gruberi strain NEG-M XP_002668802 Populus trichocarpa XP_002301340 100 100Scrippsiella trochoidea CCMP3099 CAMPEP_0115608874 77 Scrippsiella trochoidea CCMP3099 CAMPEP_0115404030 99 100 Scrippsiella trochoidea CCMP3099 CAMPEP_0115493088 100 Coccomyxa subellipsoidea C-169 XP_005644767 Chlorella variabilis XP_005845764 97 100 Chlamydomonas reinhardtii XP_001696240 98 100 Ostreococcus lucimarinus CCE9901 XP_001420849 100 Spironucleus salmonicida AFV80072 QUERY_g16482.t1_ATP_MEGiar_XP_001707581 Kipferlia bialata c10225_g1_i1 100 100 Bodo saltans CUG78626 98 Bodo saltans BS85840 Trypanosoma grayi XP_009312507 Trypanosoma vivax Y486 CCC53078 100 Trypanosoma congolense IL3000 CCC95154 99 Trypanosoma brucei gambiense DAL972 XP_011779760 96 99 100 Trypanosoma cruzi EKG07369 Trypanosoma rangeli AGN32876 100 88 Strigomonas culicis EPY18987 89 94 Leptomonas pyrrhocoris XP_015657539 Leptomonas seymouri KPI86428 100 Leishmania braziliensis MHOM/BR/75/M2904 XP_001565344 100 99 Leishmania infantum JPCM5 XP_001465906 Bodo saltans100 CUG78638 Bodo saltans BS85845 100 Trypanosoma congolense IL3000 CCC95155 100 Trypanosoma brucei brucei TREU927 XP_828620 100 Trypanosoma cruzi strain CL Brener XP_814409 57 100 Phytomonas sp. isolate EM1 CCW62514 98 Strigomonas culicis EPY29348 99 Leptomonas pyrrhocoris XP_015657540 100 Leishmania infantum ABF20292 100 Leishmania panamensis XP_010699415 100 Leishmania braziliensis MHOM/BR/75/M2904 XP_001565345 Neobodo designis100 CCAP 1951/1 CAMPEP_0174878908 100 Parasitella parasitica CEP19306 Gymnopus luxurians FD-317 M1 KIK67537 Verticillium longisporum CRK18735 98 Tuber melanosporum Mel28 XP_002836919 Botrytis cinerea T4 CCD47994 98 Aspergillus niger CBS 513.88 XP_001390670 100 Stemphylium lycopersici KNG50690 100 fungal sp. No.11243 GAM85525 90 100 100 Hordeum vulgare subsp. vulgare BAJ94832 95 Baudoinia panamericana UAMH 10762 XP_007677004 96 Aureobasidium pullulans EXF-150 KEQ83171 Sphaeroforma arctica JP61095 XP_014155244 100 Dictyostelium lacteum KYQ94253 Dictyostelium fasciculatum XP_004355233 35 Acytostelium subglobosum LB1 XP_012754946 99 Polysphondylium pallidum PN500 EFA83632 100 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168555532 89 Thecamonas trahens ATCC 50062 XP_013753218 Trichosphaerium sp Am-I-7 wt CAMPEP_0168522028 42 81 Mastigamoeba balamuthi AAN86690 33 Naegleria gruberi strain NEG-M XP_002671535 99 100 Scrippsiella trochoidea CCMP3099 CAMPEP_0115578232 26 Scrippsiella trochoidea CCMP3099 CAMPEP_0115245714 Coccomyxa subellipsoidea C-169 XP_005647736 Ostreococcus lucimarinus CCE9901 XP_001415365 37 100 Vitrella brassicaformis CCMP3155 CEM29600 78 Mayorella sp BSH-02190019 CAMPEP_0174237686 c10423_g1_i1@[email protected] Page 1 of 1

0.5 Giardia lamblia P15 EFO63329 54Kipferlia bialata c10423_g1_i1 99 QUERY_c10423_g1_i1_ISC_GrpE Kipferlia bialata g10571.t1

Armatimonadetes bacterium DC CUU36495

Mariprofundus ferrooxydans WP_009849084

78 Clostridiales bacterium CHKCI006 CVH77206

Pseudomonas sp. 21 WP_045216721

69 Bradyrhizobium viridifuturi WP_050625904

Novispirillum itersonii WP_019645098 74 Tistrella mobilis WP_014746988

81 93 Bradyrhizobium elkanii WP_038382701 Xanthobacter autotrophicus WP_012114553

66 59 Amorphus coralli WP_026319117 75 Hyphomonas sp. T16B2 WP_034826384 93 Pseudovibrio sp. FO-BEG1 AEV35172 61 80 Candidatus Liberibacter solanacearum WP_013461524 Mesorhizobium plurifarium WP_041007853 96 Hoeflea sp. IMCC20628 WP_047031461 59 90 96 Agrobacterium vitis WP_012654807 Rhizobium sp. AAP43 WP_054156284

95 Rhizobium WP_062470903

49 Rhizobium WP_024314505

Rhizobium etli WP_038686462 98 93 Rhizobium leguminosarum WP_024322195 93 70Rhizobium sp. CCGE 510 WP_007633794 Rhizobium leguminosarum WP_025415199 9978 80 63 96 Rhizobium acidisoli WP_054182041 45 Rhizobium tropici WP_047628357

Rhizobium WP_037168252

Neorhizobium galegae WP_046630407 99 Rhizobium sp. YS-1r WP_037159251 96 93 Rhizobium WP_062454031

Agrobacterium tumefaciens WP_035219247 89 98 Pantholops hodgsonii XP_005971311

91 Bradyrhizobium lupini HPC(L) EKJ95152 98 89Agrobacterium WP_020011071

Rhizobium sp. Leaf155 WP_062599194 53 Rickettsia WP_008581003

Rhodobacteraceae bacterium HLUCCO18 KPQ18281 99 Plasmodium gaboni KYN98889 44 Vitrella brassicaformis CCMP3155 CEM20488 91 Trichoplax adhaerens XP_002109822 53 Salpingoeca rosetta XP_004993586 85 Emiliania huxleyi CCMP1516 XP_005772263 100 Isochrysis aff. galbana Isogal23601_c0_seq1

62 Desulfurivibrio alkaliphilus WP_013162302

Myxococcus hansupus WP_002633345

94 Halanaerobium praevalens WP_014552832

47 64 Methanohalobium evestigatum WP_013193753

Bacteroides sp. CAG:462 CDD83565

69 Chlamydia trachomatis WP_009871747 67 Tric_XP_001329309 g60.t1@ATP@ASCT_B.com.trim.aln.trimal.treefile Page 1 of 1

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Acidimicrobium ferrooxidans DSM 10331 YP_003109446 Monosiga brevicollis MX1 XP_001744749 100 Salpingoeca rosetta XP_004995223

54 SAR202 cluster bacterium SCGC AAA240-N13 WP_017954283 Archaeoglobus fulgidus DSM 4304 NP_069974

97 Ktedonobacter racemifer WP_007919262 100Kipferlia bialata g60.t1 100 QUERY_g60.t1_ATP_ASCT_B 66 Kipferlia bialata c5818_g2_i1 57 Galdieria sulphuraria XP_005705268 Gymnodinium catenatum GC744 CAMPEP_0117468804 Ascaris suum ERG81675 88 84 100Blastocystis hominis XP_012896773 Blastocystis hominis CBK22725 58 100 Blastocystis hominis XP_012897644 100Blastocystis hominis CBK23596 Trichuris suis KFD57893 55 39 Reticulomyxa filosa ETO14272 60 Amphidinium carterae CCMP1314 CAMPEP_0176604006 100 Amphidinium carterae CCMP1314 CAMPEP_0176612656 Capsaspora owczarzaki ATCC 30864 XP_004365389 46 Spizellomyces punctatus DAOM BR117 XP_016604679

Leptospira kirschneri WP_020779209

36 Roseivirga echinicomitans KYG75406

53 Shewanella xiamenensis WP_037416917 70 28 Naegleria gruberi strain NEG-M XP_002674540 13 Runella slithyformis DSM 19594 AEI51905 43 Sedimenticola thiotaurini WP_046858048 47 99 Bacteriovorax sp. BSW11_IV WP_034727866 68 91 Cyanophora paradoxa Cpara20720

77 Lutibacter sp. BRH_c52 KUO67365

Tenacibaculum maritimum WP_024740524 41 Capnocytophaga sp. oral taxon 332 WP_009751198 99 Lutibacter sp. BRH_c52 KUO67095 100 Imtechella halotolerans WP_008238453 100 Flavobacterium tegetincola WP_026977515 99 89 Myroides odoratimimus WP_006259069

48 Flavobacterium psychrophilum WP_057671307 98 Flavobacterium psychrophilum WP_011962593 93 Flavobacterium cauense WP_023570681 61 74 88 93 Flavobacterium indicum WP_014389392

Flavobacterium succinicans WP_024981424 87 Flavobacterium sp. 316 WP_045972986 100 Nematostella vectensis XP_001621814 88 Mayorella sp BSH-02190019 CAMPEP_0174237544 78 Stereomyxa ramosa Chinc5 CAMPEP_0174268004 98 Acanthamoeba castellanii str. Neff XP_004337435 88 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168564964

Thermus tengchongensis WP_038041146

Sphaerobacter thermophilus DSM 20745 YP_003319976 56 Paraglaciecola psychrophila WP_007635299

70 Sorangium cellulosum WP_012235924 100 Chlorella variabilis XP_005848746

Eggerthella sp. YY7918 YP_004712066 g446.t1@[email protected] Page 1 of 1

0.2

61 Roseiflexus sp. RS-1 YP_001277979 Amycolatopsis mediterranei S699 YP_006547003 85 Conexibacter woesei DSM 14684 YP_003392959 80 95 Acidimicrobium ferrooxidans DSM 10331 YP_003109008 Methylacidiphilum infernorum V4 YP_001938871 93 92 Nitrosococcus oceani WP_004269204 Salinibacter ruber M8 YP_003570346 Mariprofundus ferrooxydans WP_009850259 Apibacter mensalis WP_055424550 88 87 Magnetococcus marinus WP_011715170 Candidatus Paracaedibacter acanthamoebae WP_038466986 Trichosphaerium sp Am-I-7 wt CAMPEP_0168526762 68 Octopus bimaculoides XP_014770259 23 Ancylostoma ceylanicum EYB82144 Caligus clemensi ACO15649 46 Daphnia magna KZS21457 76 75 Cyphomyrmex costatus KYN00921 94 Cimex lectularius XP_014250833 98 Tribolium castaneum XP_969318 100 48 42 91 Anopheles darlingi ETN58427 90 23 Drosophila grimshawi XP_001992752 78 Lotus japonicus AFK45219 97 Acyrthosiphon pisum XP_001946901 Trichoplax adhaerens XP_002111604 99 Ciona intestinalis XP_002128498 73 Anoplopoma fimbria ACQ58245 98 Gallus gallus XP_424129 Anolis carolinensis XP_008116383 95 Chrysemys picta bellii XP_005282702 79 Erinaceus europaeus XP_007517221 80 Felis catus XP_003995070 86 94Ceratotherium simum simum XP_004421140 35Canis lupus familiaris XP_537328 3739Aotus nancymaae XP_012302218 10Tarsius syrichta XP_008054199 8433Galeopterus variegatus XP_008588049 Equus przewalskii XP_008523284 92 2024Ovis aries XP_014959141 Homo sapiens NP_066552 Monosiga brevicollis MX1 XP_001749359 100 Salpingoeca rosetta XP_004990429 Mayorella sp BSH-02190019 CAMPEP_0174229970 Eutreptiella gymnastica NIES-381 CAMPEP_0113751062 94 Neobodo designis CCAP 1951/1 CAMPEP_0174856330 77 100 Phytomonas sp. isolate EM1 CCW63972 94 100 Leishmania braziliensis MHOM/BR/75/M2904 XP_001563793 100 100 Leishmania infantum JPCM5 XP_001464685 Trypanosoma cruzi strain CL Brener XP_816297 100 Bodo saltans BS63620 Cyanophora paradoxa Cpara18446 97 95 Chlorella variabilis XP_005849493 95 Volvox carteri f. nagariensis XP_002947050 Coccomyxa subellipsoidea C-169 XP_005645041 82 100 Vitis vinifera XP_002281655 90 Micromonas pusilla CCMP1545 XP_003062792 76 Paxillus rubicundulus Ve08.2h10 KIK95304 Acytostelium subglobosum LB1 XP_012753105 100 Dictyostelium purpureum XP_003291395 99 Guillardia theta CCMP2712 XP_005837164 85 Goniomonas sp m CAMPEP_0114560674 64 Chondrus crispus XP_005719403 91 Stereomyxa ramosa Chinc5 CAMPEP_0174259986 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168552340 65 Acanthamoeba castellanii str. Neff XP_004368142 100 Emiliania huxleyi CCMP1516 XP_005787516 52 100 Isochrysis aff. galbana Isogal5494_c0_seq1 Pavlovales sp. CCMP2436 Pavl1783_c0_seq1 Bigelowiella natans CCMP2755 Bigna_55826 79 100 96 Tetrahymena thermophila XP_001017531 100 Ichthyophthirius multifiliis XP_004030032 Paramecium tetraurelia strain d4-2 XP_001438537 80 100 100 Paramecium tetraurelia strain d4-2 XP_001347081 99 100Paramecium tetraurelia strain d4-2 XP_001439635 Corallomyxa tenera Corten19778_c0_seq1 100Phytophthora parasitica P1976 ETO72884 100 Plasmopara halstedii CEG42371 24 100Aphanomyces invadans XP_008867524 92 Saprolegnia parasitica CBS 223.65 XP_012194612 100 Albugo candida CCI43451 Blastocystis hominis CBK22400 73 Nannochloropsis gaditana EWM30264 100 100 Aureococcus anophagefferens XP_009033508 Aureococcus anophagefferens Auran_59800 83 Thalassiosira pseudonana CCMP1335 XP_002291809 99 Gymnodinium catenatum GC744 CAMPEP_0117464720 72 Ectocarpus siliculosus CBN75203 100 Aciduliprofundum boonei T469 YP_003483177 Dehalococcoides mccartyi 195 YP_181466 61 Hydrogenobacter thermophilus TK-6 YP_003433328 95 Dehalococcoides mccartyi 195 YP_181190 94 Methanobacterium sp. AL-21 YP_004291704 88 42 Thermotoga maritima MSB8 YP_007977779 61 Thermococcus kodakarensis KOD1 YP_184027 Tric_XP_001312168 Kipferlia bialata g446.t1 Kipferlia bialata c5930_g1_i1 QUERY_g446.t1_GCS_NuoE g1346.t1@[email protected] Page 1 of 1

0.2 100 Chroococcidiopsis thermalis PCC 7203 YP_007093732 31 Anaerolinea thermophila UNI-1 YP_004174115 69 54 Salpingoeca rosetta XP_004989330 Acidimicrobium ferrooxidans DSM 10331 YP_003109726 Deinococcus gobiensis I-0 YP_006259868 marine actinobacterium PHSC20C1 WP_009774234 34 39 Methylacidiphilum infernorum V4 YP_001940818 Verrucomicrobiae bacterium DG1235 WP_008103885 28 75 99 Pelodictyon phaeoclathratiforme BU-1 YP_002019170 95 Hydrogenobacter thermophilus TK-6 YP_005511299 37 Candidatus Caldiarchaeum subterraneum YP_008797909 Thermococcus kodakarensis KOD1 YP_184448 Aciduliprofundum boonei T469 YP_003482842 94 98 Bacillus thuringiensis Bt407 YP_006928988 Microcoleus sp. PCC 7113 YP_007121715 Kipferlia bialata g481.t1 100 QUERY_g1346.t1_GCS_GCST Kipferlia bialata g1346.t1 Vitrella brassicaformis CCMP3155 CEM22864 90 99 100 Neospora caninum Liverpool XP_003884724 Toxoplasma gondii ME49 XP_002369089 Plasmodium chabaudi chabaudi XP_742683 69 69 Perkinsus marinus ATCC 50983 XP_002788645 100 Thalassiosira oceanica EJK66549 100 Gymnodinium catenatum GC744 CAMPEP_0117602312 Ectocarpus siliculosus CBJ48669 90 Aureococcus anophagefferens Auran_20469 100 97 Blastocystis hominis CBK22390 Galdieria sulphuraria XP_005702615 98 Ichthyophthirius multifiliis XP_004032084 59 Paramecium tetraurelia strain d4-2 XP_001457410 100 Aphanomyces astaci XP_009830163 Plasmopara halstedii CEG46203 100 Phytophthora sojae XP_009527269 60 83 100 Phytophthora parasitica INRA-310 XP_008915613 100 Phytophthora infestans T30-4 XP_002898763 100 100 Oxytricha trifallax EJY83359 Stylonychia lemnae CDW71112 88 Neobodo designis CCAP 1951/1 CAMPEP_0174880676 100 Trypanosoma brucei brucei strain 927/4 GUTat10.1 XP_829031 77 99 Trypanosoma cruzi strain CL Brener XP_808051 100 Leishmania infantum JPCM5 XP_001469634 74 Leishmania braziliensis MHOM/BR/75/M2904 XP_001569023 99 100 Bodo saltans BS71935 100 Bigelowiella natans CCMP2755 Bigna_92765 Euglena gracilis Euggra287_c0_seq2 Eutreptiella gymnastica NIES-381 CAMPEP_0113752368 100100 Chrysochromulina sp. CCMP291 KOO24557 100 Emiliania huxleyi CCMP1516 XP_005760444 Isochrysis aff. galbana Isogal29226_c0_seq1 96 100 Pavlovales sp. CCMP2436 Pavl466_c0_seq1 Reticulomyxa filosa ETO25443 45 96 98 Poecilia mexicana XP_014863531 85 Fopius arisanus XP_011301654 56 Capsaspora owczarzaki ATCC 30864 XP_004365723 Mucor circinelloides f. circinelloides 1006PhL EPB81805 Trichosphaerium sp Am-I-7 wt CAMPEP_0168531616 55 Mayorella sp BSH-02190019 CAMPEP_0174237254 77 Trichoderma atroviride IMI 206040 XP_013943506 73 100 Pyrodinium bahamense pbaha01 CAMPEP_0179187200 100 Pyrodinium bahamense pbaha01 CAMPEP_0179068252 65 66 Symbiodinium sp. Mp CAMPEP_0169878116 Guillardia theta CCMP2712 XP_005827685 69 100 Glycine max XP_003529643 Ostreococcus lucimarinus CCE9901 XP_001420189 100 Auxenochlorella protothecoides XP_011396728 90 88 Gonium pectorale KXZ44287 82 Chlorella variabilis XP_005849287 Coccomyxa subellipsoidea C-169 XP_005648064 49 100 62 Stereomyxa ramosa Chinc5 CAMPEP_0174262062 Acanthamoeba castellanii str. Neff XP_004351581 Goniomonas sp m CAMPEP_0114559426 87 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168556978 100 Dictyostelium fasciculatum XP_004367030 Polysphondylium pallidum PN500 EFA84814 95 Acytostelium subglobosum LB1 XP_012758837 100 100Dictyostelium purpureum XP_003294508 91 Pleomorphomonas oryzae WP_026790938 Parvibaculum lavamentivorans WP_011995602 85 Beijerinckia mobilis WP_034995892 Nesiotobacter exalbescens WP_028482536 Oceanicaulis alexandrii WP_022701669 99 96 Asticcacaulis biprosthecium WP_040309485 99 Ricinus communis XP_002538372 84 100 Magnetospira sp. QH-2 WP_046020493 40 Rhodospirillum centenum WP_041785517 38 Nisaea denitrificans WP_028466826 Leisingera sp. ANG-DT WP_039148662 44 Elioraea tepidiphila WP_019013507 100 Reyranella massiliensis WP_020698021 87 Bradyrhizobium sp. WSM2793 WP_018316618 34 96 Acetobacter okinawensis WP_025828910 Candidatus Rhodobacter lobularis WP_049645625 uncultured Rhizobium sp. HF0500_10F10 ADI22563 83 Martelella mediterranea WP_018063260 51 96 Rhizobium WP_037163290 100 Rhizobium selenitireducens WP_028735075 100 Neorhizobium galegae WP_038543156 78 Agrobacterium tumefaciens WP_003512949 60 70 89Rhizobium gallicum WP_039846768 69 Rhizobium sp. IBUN WP_025661164 Rhizobium sp. CF142 WP_007826143 100 Rhizobium tropici WP_047638207 88 Ricinus communis XP_002538328 100Delftia acidovorans WP_016451507 Pseudomonas sp. CFII68 WP_018614217 89 Balneatrix alpica WP_027313416 97 Neptuniibacter caesariensis WP_007023080 95 Photobacterium sp. JCM 19050 WP_054675125 100 Photobacterium sanctipauli WP_036818452 Vibrio alginolyticus WP_053312049 94 97 Vibrio renipiscarius WP_040992137 81 Vibrio sp. C7 GAL16102 100 Vibrio sp. EJY3 WP_014234300 Vibrio parahaemolyticus WP_053808456 99 100Vibrio alginolyticus WP_054577865 96Vibrio parahaemolyticus WP_062858772 Vibrio parahaemolyticus WP_021821640 100 Vibrio parahaemolyticus WP_025579971 6986Vibrio parahaemolyticus WP_047733766 9116Vibrio parahaemolyticus WP_029834505 28Vibrio parahaemolyticus WP_047725216 99 97Vibrio parahaemolyticus WP_025633878 10Vibrio parahaemolyticus WP_062852828 15Vibrio parahaemolyticus WP_053305764 92 Vibrio mytili WP_041153886 Vibrio campbellii ATCC BAA-1116 YP_008528951 g2569.t1@[email protected] Page 1 of 1

0.5

70 Pelodictyon phaeoclathratiforme BU-1 YP_002017859 70 Eggerthella sp. YY7918 WP_013978659 57 Archaeoglobus fulgidus DSM 4304 NP_071137 Methanobacterium sp. AL-21 WP_013645715 Dehalococcoides mccartyi 195 YP_181151 100 Kipferlia bialata g2569.t1 100 QUERY_g2569.t1_anti-oxid_Rbr Kipferlia bialata g1163.t1 58 100 Kipferlia bialata g2290.t1 51 98Kipferlia bialata c5984_g1_i1 Dysgonomonas gadei WP_006799462 48 100 Bacteroides eggerthii WP_004291818 Sanguibacteroides WP_041502387 34 Porphyromonas bennonis WP_018107070 82 Lentimicrobium saccharophilum WP_062037232 30 Akkermansia sp. CAG:344 CDD93241 55 Mesotoga prima WP_014731759 Clostridium botulinum WP_041350334 3958 uncultured bacterium EKE04656 56 Halonatronum saccharophilum WP_027340637 Psychrilyobacter atlanticus WP_028854998 62 Endomicrobium proavitum WP_052571249 Syntrophus aciditrophicus SB ABC78317 55 92 Arc I group archaeon ADurb1013_Bin02101 KYC53271 Desulfotomaculum sp. 46_296 KUK54197 88 90 Methanosalsum zhilinae WP_013899040 50 Desulfotomaculum gibsoniae WP_052543956 58 Moorella thermoacetica WP_011392800 delta proteobacterium HotSeep1 AMM39991 92 69 Thermoplasmatales archaeon DG-70-1 KYK28146 80 Candidatus Thorarchaeota archaeon SMTZ1-83 KXH69867 Coxiella sp. DG_40 KPJ66132 73 89 85 Desulfovibrio magneticus WP_012750412 Thermodesulfobacterium commune WP_038060158 38 88 Methanosaeta harundinacea WP_014587372 Desulfuromonas sp. TF WP_035055537 51 74 Desulfovermiculus halophilus WP_027369402 48 delta proteobacterium NaphS2 WP_006423105 Planctomycetes bacterium SM23_65 KPL03376 65 94 Treponema primitia WP_010255723 95 98 Desulfobacula sp. TS WP_031451152 Thermovirga lienii WP_014162393 100 96 Desulfovibrio aespoeensis Aspo-2 ADU61823 82 Desulfobulbus japonicus WP_028579509 99 40 Desulfovibrio africanus WP_005987301 73 89 Desulfomicrobium escambiense WP_028576899 97 Sedimenticola thiotaurini WP_046860043 Euryarchaeota archaeon SG8-5 KYK26935 96 candidate division TA06 bacterium DG_24 KPJ53066 99 Desulfonauticus sp. 38_4375 KUJ95673 Candidatus Omnitrophus magneticus KJJ85427 98 Methanoregula formicica WP_015284420 100 Methanolinea sp. SDB KQC11140 miscellaneous Crenarchaeota group-6 archaeon AD8-1 KON33521 98 Candidatus Bathyarchaeota archaeon B63 KYH41304 79 92 Methanospirillum hungatei WP_011448328 83 Smithella WP_037378405 84 75 Candidatus Methanoperedens sp. BLZ1 KPQ45173 Thermoplasmatales archaeon SG8-52-1 KYK27307 74 candidate division Zixibacteria bacterium SM1_73 KPL05062 57 99 candidate divison MSBL1 archaeon SCGC-AAA261D19 KXB02743 Hadesarchaea archaeon YNP_45 KUO39623 44 61 Actinobacteria bacterium 66_15 KUK48124 64 unclassified Peptostreptococcaceae (miscellaneous) WP_009527933 Methanobacterium sp. AL-21 YP_004290153 100 76 Entamoeba dispar SAW760 XP_001734442 90 Entamoeba histolytica HM-1:IMSS XP_652131 39 100 Entamoeba invadens IP1 XP_004260064 79 Tric_XP_001320125 56 [Ruminococcus] gnavus WP_009244495 Caldicellulosiruptor sp. Wai35.B1 WP_045173571 61 Desulfotomaculum thermocisternum WP_027356157 19 candidate division TA06 bacterium 32_111 KUK51418 Synergistaceae WP_008711668 98 Thermoplasmatales archaeon SCGC AB-540-F20 EMR75233 Geobacter sp. M21 WP_015837207 Desulfosporosinus sp. OT WP_009623251 59 80 Subdoligranulum variabile WP_007045590 69 Eubacterium sp. CAG:274 CDC21482 79 Prevotella stercorea WP_007901186 Coprobacillus sp. CAG:826 CDD93027 66 89 methanogenic archaeon mixed culture ISO4-G1 AMK14170 90 [Clostridium] methylpentosum WP_006354878 Burkholderia multivorans ATCC 17616 YP_001947998 44 Trichosphaerium sp Am-I-7 wt CAMPEP_0168529996 69 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168556108 33 Guillardia theta CCMP2712 XP_005839869 96 80 Goniomonas sp m CAMPEP_0114556680 Hydrogenobacter thermophilus TK-6 YP_005511895 91 Stereomyxa ramosa Chinc5 CAMPEP_0174252794 62 77 Candidatus Nitrospira defluvii WP_013250530 Astrolonche sp. RhEST0030242 g3752.t1@[email protected] Page 1 of 1

0.2 100 Trichomonas vaginalis G3 XP_001309666 Trichomonas vaginalis G3 XP_001299092 Trichomonas vaginalis G3 XP_001298652 99 94 Trichomonas vaginalis G3 XP_001316602 94 Trichomonas vaginalis G3 XP_001313356 84 92 Coptotermes formosanus AFZ78677 88Trichomonas vaginalis G3 XP_001330099 Bigelowiella natans CCMP2755 Bigna_49647 61 Mayorella sp BSH-02190019 CAMPEP_0174231416 Corallomyxa tenera Corten1938_c0_seq1 63 Trichosphaerium sp Am-I-7 wt CAMPEP_0168537482 37 Biomphalaria glabrata AAK26236 96 Polysphondylium pallidum PN500 EFA80375 Acytostelium subglobosum LB1 XP_012750146 100 Dictyostelium discoideum AX4 XP_644051 29Kipferlia bialata c5409_g1_i1 100 QUERY_g3752.t1_anti-oxid_TrxP 100 Kipferlia bialata g3752.t1 76 Spironucleus salmonicida EST47325 100 Giar_ESU45818 Salpingoeca rosetta XP_004995282 64 91 Ancylostoma ceylanicum 4FH8_A Ciona intestinalis NP_001027810 Caligus clemensi ACO15457 85 Larimichthys crocea XP_010749164 54 Pseudopodoces humilis XP_005521167 57 Xenopus laevis NP_001085178 86 Ornithorhynchus anatinus XP_007664543 75 Monodelphis domestica XP_001363120 76 100 Homo sapiens AAA50464 16 95 Pteropus vampyrus XP_011365893 synthetic construct other AIC54860 88Microtus ochrogaster XP_005348516 2594Ovis aries musimon XP_011987125 40 7Equus caballus XP_001496084 21Microcebus murinus XP_012631469 89Macaca mulatta NP_001247862 27Miniopterus natalensis XP_016054746 Homo sapiens 4XCS_A 37Homo sapiens NP_001189360 Gekko japonicus XP_015271321 48 Sarcophilus harrisii XP_003760721 95 Sus scrofa NP_001231403 100 Tursiops truncatus XP_004318885 81 Leptonychotes weddellii XP_006730608 36 Chrysochloris asiatica XP_006874320 62 Condylura cristata XP_004688772 90 Otolemur garnettii XP_003797966 20Tarsius syrichta XP_008048317 61 Mesocricetus auratus XP_005087380 9981Callithrix jacchus XP_002761827 Aotus nancymaae XP_012291308 30 10026Pongo abelii XP_009251113 24Homo sapiens AAA50465 100100synthetic construct other AAX36919 Homo sapiens 1QMV_A 6synthetic construct other CCV02865 32synthetic construct other AAX37153 35 synthetic construct other AAX29764 synthetic construct other AAY18909 Papio anubis XP_009191878 Homo sapiens NP_005800 Bombyx mori XP_012544347 Phytophthora infestans T30-4 XP_002898434 26 Naegleria gruberi strain NEG-M XP_002678814 100 Phytomonas sp. isolate EM1 CCW65167 72 Trypanosoma cruzi strain CL Brener XP_804571 100 Neobodo designis CCAP 1951/1 CAMPEP_0174826822 100 Bodo saltans BS28140 39 Auxenochlorella protothecoides XP_011399388 Thecamonas trahens ATCC 50062 XP_013752659 30 97 Equus caballus XP_001493666 99 Ciona intestinalis XP_002126758 Rhizopus microsporus CEI95878 Chlamydomonas reinhardtii XP_001699660 67 Leptospira interrogans WP_001136732 59 Chlamydomonas reinhardtii XP_001696611 100 Prochlorococcus marinus str. MIT 9313 NP_894586 66 72 Spizellomyces punctatus DAOM BR117 XP_016610559 64 54 Batrachochytrium dendrobatidis JAM81 XP_006677507 62 58 Denitrovibrio acetiphilus DSM 12809 YP_003504986 Blastocystis hominis CBK25198 50 54 Acanthamoeba castellanii str. Neff XP_004333640 70 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168559484 Euglena gracilis Euggra735_c0_seq1 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168556296 63 Batrachochytrium dendrobatidis JAM81 XP_006678906 49 22 Acanthamoeba castellanii str. Neff XP_004348542 63 Neobodo designis CCAP 1951/1 CAMPEP_0174850228 Thelohanellus kitauei KII61746 g4112.t1@[email protected] Page 1 of 1

0.2

41 Coxiella burnetii WP_040953557

Arcobacter anaerophilus WP_044418472

Thiorhodococcus sp. AK35 WP_043750646

Bacteroides salanitronis WP_013618226

40 91 Anaerobiospirillum succiniciproducens WP_027939495 83 Coxiella sp. DG_40 KPJ67774 38 uncultured bacterium EKE01783 Coptotermes formosanus AFS41415 100 Trichomonas vaginalis G3 XP_001317169 77 85 Trichomonas vaginalis G3 XP_001320923 87 Tritrichomonas foetus AAC47734 Leptomonas seymouri KPI90647 100 90 Leishmania infantum JPCM5 XP_001463371 94 Leishmania panamensis XP_010704161 100 Leishmania braziliensis MHOM/BR/75/M2904 XP_001562094 80 75 Angomonas deanei EPY27087 Angomonas deanei EPY37688

74 Leptomonas seymouri KPI90097 100 Leishmania braziliensis MHOM/BR/75/M2904 XP_001567639 100Leishmania infantum JPCM5 XP_001467953 96 53 Phytomonas sp. isolate EM1 CCW60383 81 Trypanosoma cruzi strain CL Brener XP_815674 89 Bodo saltans BS43150

Leptolyngbya valderiana WP_063719021 Kipferlia bialata g4112.t1 QUERY_g4112.t1_anti-oxid_SOD 100 Kipferlia bialata g4116.t1

84 Kipferlia bialata c4463_g1_i2 34Kipferlia bialata g6810.t1 100 Kipferlia bialata g7145.t1 76 Kipferlia bialata c4115_g1_i1

70 Abiotrophia defectiva ATCC 49176 ESK66579 85 Baudoinia panamericana UAMH 10762 XP_007671847

Buchnera aphidicola WP_053940162 74 98 Pantoea sp. IMH WP_024966332

44 50 Gilliamella apicola WP_025314324 Stenotrophomonas maltophilia WP_053498542

87 Rhodopirellula sallentina SM41 EMI54768

96 Pirellula sp. SH-Sr6A AMV33027

43 Deinococcus actinosclerus WP_062158124

Lysinibacillus sp. BF-4 WP_036141953

Staphylococcus xylosus AAS78511

48 77 Planomicrobium sp. ES2 WP_052651770 87 49 Bacillus subterraneus WP_044393138 37 Geobacillus sp. 12AMOR1 WP_047818706 81 Paenibacillus sp. AIU 311 BAH79725 91 Microchaete sp. PCC 7126 WP_017651914 85 100 Microgenomates group bacterium GW2011_GWA2_44_7 KKT74520 82 Thermoactinomyces vulgaris WP_022737678

Patulibacter medicamentivorans WP_007577370

66 Flavobacterium cauense WP_023569512

99 Nematostella vectensis XP_001619321 92 Zobellia galactanivorans WP_013992947 68 Afipia WP_009337462 Chlamydomonas reinhardtii XP_001690591 g4595.t1@[email protected] Page 1 of 1

0.2 100 99 Trichoplax adhaerens XP_002119095 Candidatus Nitrospira defluvii WP_013247432 Rhodobacteraceae bacterium HLUCCA12 KPQ06316 Mariprofundus ferrooxydans WP_009850456 99 Shigella sonnei Ss046 YP_309671 95 Amycolatopsis mediterranei S699 YP_006550996 Methanobacterium sp. AL-21 YP_004291590 55 Candidatus Caldiarchaeum subterraneum YP_008797521 100 84 Candidatus Nitrosopumilus sp. AR2 YP_006776043 61 82 Aciduliprofundum boonei T469 YP_003483357 81 Bacteroides thetaiotaomicron VPI-5482 NP_809701 Caldilinea aerophila DSM 14535 = NBRC 104270 YP_005441738 99 Denitrovibrio acetiphilus DSM 12809 YP_003505063 Methylacidiphilum infernorum V4 YP_001939698 100 Kipferlia bialata g4595.t1 QUERY_g4595.t1_ATP_SCSb 57 Euglena gracilis Euggra2153_c0_seq1 Perkinsela sp. CCAP 1560/4 KNH05889 100 Trypanosoma grayi XP_009308958 Trypanosoma rangeli SC58 ESL05385 96 Trypanosoma cruzi marinkellei EKF31549 100 81 Trypanosoma cruzi strain CL Brener XP_818583 100Trypanosoma cruzi EKG03900 99 100Trypanosoma cruzi strain CL Brener XP_802641 100Phytomonas sp. isolate EM1 CCW60153 Leishmania infantum JPCM5 XP_001469702 100 Leishmania panamensis XP_010703239 100 100 Leishmania braziliensis MHOM/BR/75/M2904 XP_001568936 Bodo100 saltans BS05780 56 Neobodo designis CCAP 1951/1 CAMPEP_0174840296 100 97 97 Coptotermes formosanus AGM32326 Coptotermes formosanus AGM32712 Trichomonas vaginalis G3 XP_001584099 100 16 Blastocystis hominis CBK24221 100 Euglena gracilis Euggra722_c0_seq2 Eutreptiella gymnastica NIES-381 CAMPEP_0113754346 Monosiga brevicollis MX1 XP_001743732 99 Salpingoeca rosetta XP_004997449 100 97 Aspergillus clavatus NRRL 1 XP_001270536 Theileria parva strain Muguga XP_766198 89 Babesia equi XP_004833199 100 96 Ichthyophthirius multifiliis XP_004031070 100 Tetrahymena thermophila XP_001015794 74 Ichthyophthirius multifiliis XP_004037542 Tetrahymena thermophila XP_001017756 100 100 Paramecium tetraurelia strain d4-2 XP_001440162 56 84 Plasmodiophora brassicae CEO94714 Corallomyxa tenera Corten19501_c0_seq1 Bigelowiella natans CCMP2755 Bigna_66428 73 92 Naegleria gruberi strain NEG-M XP_002679382 55 Sawyeria marylandensis ABU95433 50 Cyanophora paradoxa Cpara18663 Chondrus crispus XP_005718350 47 90 Guillardia theta CCMP2712 XP_005824953 Goniomonas sp m CAMPEP_0114558692 Bathycoccus prasinos XP_007511494 96 Dorcoceras hygrometricum KZV32970 62 Chlamydomonas reinhardtii XP_001691582 42 Auxenochlorella protothecoides XP_011401590 32 100 97 Coccomyxa subellipsoidea C-169 XP_005643933 7098 Thalassiosira oceanica EJK65694 95 Pavlovales sp. CCMP2436 Pavl741_c0_seq1 Emiliania huxleyi CCMP1516 XP_005768701 95 Isochrysis aff. galbana Isogal38647_c0_seq1 100 99 Aureococcus anophagefferens XP_009038573 63 Aureococcus anophagefferens Auran_28673 100 Aphanomyces100 invadans XP_008880547 100 Plasmopara halstedii CEG47659 Phytophthora infestans T30-4 XP_002902934 100 Gymnodinium catenatum GC744 CAMPEP_0117480282 Ectocarpus siliculosus CBJ26646 95 Nannochloropsis gaditana EWM24300 71 Thalassiosira pseudonana CCMP1335 XP_002286405 92 97 Vitrella brassicaformis CCMP3155 CEL98573 72 Plasmodium chabaudi chabaudi XP_745223 Neospora caninum Liverpool XP_003884989 68 87 Toxoplasma gondii ME49 XP_002364222 100100 Prorocentrum minium CCMP2233 CAMPEP_0176686810 98 Prorocentrum minium CCMP2233 CAMPEP_0176872888 Prorocentrum minium CCMP2233 CAMPEP_0176992414 30 Perkinsus marinus ATCC 50983 XP_002769505 46 100 Perkinsus marinus ATCC 50983 XP_002783211 100 Allomyces macrogynus ATCC 38327 KNE54111 Neocallimastix patriciarum AAP83351 95 Capsaspora owczarzaki ATCC 30864 XP_004349845 98 Saccharomyces cerevisiae YJM1415 AJR98551 Plicaturopsis crispa FD-325 SS-3 KII93638 Chaetomium thermophilum var. thermophilum DSM 1495 XP_006691074 97 Scedosporium apiospermum XP_016641669 Colletotrichum gloeosporioides Cg-14 EQB50853 38 99 Stachybotrys chartarum IBT 7711 KEY69536 100 94 Fusarium pseudograminearum CS3096 XP_009261212 92 Drechmeria coniospora KYK55876 89 Isaria fumosorosea ARSEF 2679 OAA52911 84 Metarhizium rileyi RCEF 4871 OAA46220 66 41 Aschersonia aleyrodis RCEF 2490 OAA33716 100 Ustilaginoidea virens GAO17714 87 79Metarhizium brunneum ARSEF 3297 XP_014545811 3422Metarhizium robertsii ARSEF 23 XP_007818845 99 38Metarhizium guizhouense ARSEF 977 KID88799 100 98Metarhizium majus ARSEF 297 XP_014581537 Marssonina26 brunnea f. sp. ’multigermtubi’ MB_m1 XP_007292231 76 Talaromyces stipitatus ATCC 10500 XP_002482345 19 Arthrobotrys oligospora ATCC 24927 XP_011118834 94 Verruconis gallopava XP_016217992 98 96 Dothistroma septosporum NZE10 EME41937 79 Pneumocystis murina B123 XP_007873974 Rhizopus microsporus CEG64582 Rhizophagus irregularis DAOM 181602 ESA20134 100 Daphnia pulex Dappu_304522 Thecamonas trahens ATCC 50062 XP_013755650 49 Stereomyxa ramosa Chinc5 CAMPEP_0174271440 38 Acanthamoeba castellanii str. Neff XP_004368306 92100 Trichosphaerium sp Am-I-7 wt CAMPEP_0168516686 Mayorella sp BSH-02190019 CAMPEP_0174229602 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168555650 100 Dictyostelium fasciculatum XP_004361979 79 Polysphondylium pallidum PN500 EFA82949 100 Acytostelium subglobosum LB1 XP_012750993 100 100Dictyostelium purpureum XP_003293744 89 Sphaeroforma arctica JP610 XP_014157510 Trichosphaerium sp Am-I-7 wt CAMPEP_0168536870 72 Stereomyxa ramosa Chinc5 CAMPEP_0174261214 Mayorella sp BSH-02190019 CAMPEP_0174229018 95 Dictyostelium fasciculatum XP_004361769 99 Acytostelium subglobosum LB1 XP_012752753 93 76 Polysphondylium pallidum PN500 EFA83861 100 94 Dictyostelium purpureum XP_003291936 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168558474 81 Thecamonas trahens ATCC 50062 XP_013761585 Capsaspora owczarzaki ATCC 30864 XP_004348448 88 Caenorhabditis briggsae XP_002632430 87 Monosiga brevicollis MX1 XP_001744342 55 Salpingoeca rosetta XP_004996763 100 100 100 Ichthyophthirius multifiliis XP_004032120 Tetrahymena thermophila XP_001023435 Paramecium tetraurelia strain d4-2 XP_001460008 65 Plasmodiophora brassicae CEP03572 88 Reticulomyxa filosa ETO14279 95 Bigelowiella natans CCMP2755 Bigna_92245 g5315.t1@[email protected] Page 1 of 1

0.2 Cyanophora paradoxa Cpara21729 100 Chlorella variabilis XP_005851548 75 Chlamydomonas reinhardtii XP_001694454 Reticulomyxa filosa ETO28447 99 Reticulomyxa filosa ETO28446 98 Guillardia theta CCMP2712 XP_005832553 98 Pyrodinium bahamense pbaha01 CAMPEP_0179143312 100 Symbiodinium sp. KB8 SyKB8_6311_c0_seq1 100 Symbiodinium sp. Mp CAMPEP_0169786850 95 Mayorella sp BSH-02190019 CAMPEP_0174236182 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168550582 27 78 Euglena gracilis Euggra1177_c0_seq1 Cyanophora paradoxa Cpara00604 85 Thalassiosira pseudonana CCMP1335 XP_002288422 16 Porphyridium purpureum Porpur_2122.4 48 Nannochloropsis gaditana CCMP526 XP_005853562 39 85 Acanthamoeba castellanii str. Neff XP_004342370 Vibrio hepatarius WP_053407192 61 Methylocaldum szegediense WP_026609864 Acidiphilium sp. PM WP_039888806 Thermobispora bispora WP_013130407 100 99 Paraburkholderia mimosarum WP_028230039 Burkholderia vietnamiensis WP_059703900 99 100 68Burkholderia ubonensis WP_059660956 61Burkholderia ubonensis WP_060029050 100 Burkholderia ubonensis WP_059954745 36 100 92Burkholderia ubonensis WP_059552964 10092Burkholderia ubonensis WP_059941217 Burkholderia ubonensis WP_059981921 19 69Burkholderia ubonensis WP_059902860 Methylobacter sp. BBA5.1 WP_036247554 100 Succinispira mobilis WP_019879115 Aminiphilus circumscriptus WP_029165776 Pelobacter propionicus WP_011735124 79 100 Thermotoga maritima MSB8 YP_007977528 Methanobacterium sp. AL-21 YP_004291329 71 100 Bacillus thuringiensis Bt407 YP_006928126 Peptoclostridium difficile WP_054274116 100 96 92 Fusobacterium nucleatum WP_029600054 Trichomonas vaginalis G3 XP_001325705 97 69 Hippea maritima WP_013681143 100 Desulfurella acetivorans A63 AHF97270 96 Pseudothermotoga thermarum WP_013932641 73 Methanocaldococcus jannaschii DSM 2661 NP_247750 Desulfotignum balticum WP_024333966 64 53 69 54 Nitrospira bacterium SG8_35_1 KPK35957 Methanocella conradii HZ254 AFD01185 60 45 Desulfomonile tiedjei DSM 6799 AFM25199 50 Desulfobulbus propionicus WP_015724155 53 Desulfatibacillum alkenivorans WP_015947108 Desulfovibrio brasiliensis WP_054651573 96 98 Dethiobacter alkaliphilus AHT 1 EEG78089 Thermodesulfovibrio yellowstonii WP_012545464 Thermodesulfobium narugense WP_013756917 72 100 Entamoeba invadens IP1 XP_004259188 Entamoeba invadens IP1 XP_004255625 100 63 100 Entamoeba invadens IP1 XP_004185761 Entamoeba dispar SAW760 XP_001739218 Entamoeba histolytica HM-1:IMSS XP_650006 78 100 Thermodesulfatator indicus WP_013907633 85 Thermosulfidibacter takaii ABI70S6 BAT71492 76 Cyanothece sp. PCC 7822 YP_003888691 Pleomorphomonas koreensis WP_026782198 71 Opitutaceae bacterium TAV1 WP_007363541 99 Giar_AF458335_1 Spironucleus salmonicida AFV80086 38Kipferlia bialata c5108_g2_i1 100 Kipferlia bialata g5315.t1 95 QUERY_g5315.t1_anti-oxid_HCP Kipferlia bialata c3709_g1_i1 Kipferlia bialata g9057.t1 100 99 Kipferlia bialata g11549.t1 100 Kipferlia bialata c3612_g2_i1 g5570.t1@[email protected] Page 1 of 1

0.5

98 Candidatus Nitrososphaera gargensis Ga9.2 YP_006863244 Geobacillus toebii KYD32543 Sphingobacterium sp. 21 WP_013665841 81 Clostridium sp. KLE 1755 WP_021634684 Bacteroides thetaiotaomicron VPI-5482 NP_812098 90 Ostreococcus lucimarinus CCE9901 XP_001417363 100 Chlorella variabilis XP_005846382 Bigelowiella natans CCMP2755 Bigna_92195 100 Arabidopsis lyrata subsp. lyrata XP_002868156 96 100 100 Pavlovales sp. CCMP2436 Pavl779_c0_seq1 100 Thalassiosira pseudonana CCMP1335 XP_002296642 100 Ectocarpus siliculosus CBN79667 100 Porphyridium purpureum Porpur_2268.4 100 Galdieria sulphuraria XP_005703093 Microcoleus sp. PCC 7113 YP_007124726 99 Cyanobium gracile PCC 6307 YP_007045850 60 Gloeobacter violaceus PCC 7421 NP_925975 88 60 Amycolatopsis mediterranei S699 YP_005530157 47 Spirochaeta smaragdinae DSM 11293 YP_003802327 Candidatus Nitrospira defluvii WP_013249254 Roseiflexus sp. RS-1 WP_011957711 36 Pelodictyon phaeoclathratiforme BU-1 YP_002018646 60 91 Opitutaceae bacterium TAV1 WP_007363008 97 Zobellia galactanivorans YP_004734985 91 100 Mariprofundus ferrooxydans WP_009850092 100 Bradyrhizobium sp. BTAi1 WP_012044491 100 Trichoplax adhaerens XP_002118664 100 Thermotoga maritima MSB8 YP_008990647 Sebaldella termitidis ATCC 33386 YP_003306845 3850 Candidatus Caldiarchaeum subterraneum WP_020864665 Acidimicrobium ferrooxidans DSM 10331 YP_003109445 Natrialba magadii ATCC 43099 YP_003479803 99 Enterobacteriaceae bacterium 9_2_54FAA WP_020303577 53 Parachlamydia acanthamoebae UV-7 YP_004652036 100 Kipferlia bialata g4376.t1 74 Kipferlia bialata c9266_g1_i1 57 Leisingera sp. ANG-M1 WP_039168465 Candidatus Paracaedibacter acanthamoebae WP_038463129 26 Tistrella mobilis WP_014746519 Magnetospirillum gryphiswaldense MSR-1 CAM74891 endosymbiont of Acanthamoeba sp. UWC8 WP_038537790 100 Guillardia theta CCMP2712 XP_005829230 Goniomonas sp m CAMPEP_0114554398 35 Sawyeria marylandensis ABU95431 Perkinsus marinus ATCC 50983 XP_002786931 76 Plasmodium chabaudi chabaudi XP_742153 77 Naegleria gruberi strain NEG-M XP_002680533 79 40 59 Eutreptiella gymnastica NIES-381 CAMPEP_0113735164 80 Neobodo designis CCAP 1951/1 CAMPEP_0174827750 52 Bodo saltans BS14355 87 96 Trypanosoma cruzi strain CL Brener XP_812294 100 Trypanosoma brucei brucei TREU927 XP_829730 100 Angomonas deanei EPY31444 100 99 Leptomonas pyrrhocoris XP_015663408 Leishmania braziliensis MHOM/BR/75/M2904 XP_001567711 100 Leishmania infantum JPCM5 XP_001468025 56 100100 Phytomonas sp. isolate EM1 CCW60315 100 Stylonychia lemnae CDW79133 100 Oxytricha trifallax EJY72722 96 Oxytricha trifallax EJY85909 Stylonychia lemnae CDW75606 69 100 Paramecium tetraurelia strain d4-2 XP_001457469 100 83 Pseudocohnilembus persalinus KRX01420 50 100 Ichthyophthirius multifiliis XP_004036395 100 Tetrahymena thermophila XP_001014580 3844 100 Vitrella brassicaformis CCMP3155 CEM23213 Eimeria necatrix XP_013433469 Neospora caninum Liverpool XP_003881389 100 100 Toxoplasma gondii ME49 XP_002367828 84 100 Theileria parva strain Muguga XP_763245 Babesia equi XP_004832497 72 100 Reticulomyxa filosa ETO22502 Corallomyxa tenera Corten1469_c0_seq2 Bigelowiella natans CCMP2755 Bigna_87173 8276 Chondrus crispus XP_005713748 Pavlovales sp. CCMP2436 Pavl433_c0_seq1 43 Chrysochromulina sp. CCMP291 KOO30081 100 Isochrysis aff. galbana Isogal16921_c0_seq1 100 Emiliania huxleyi CCMP1516 XP_005784168 61 100 Blastocystis hominis CBK21392 100 Saprolegnia diclina VS20 XP_008610979 Phytophthora infestans T30-4 XP_002898325 78 100 Gymnodinium catenatum GC744 CAMPEP_0117466756 96 86 Gymnodinium catenatum GC744 CAMPEP_0117621088 Nannochloropsis gaditana EWM29624 99 Aureococcus anophagefferens XP_009037037 93 Aureococcus anophagefferens Auran_26536 100 100 Ectocarpus siliculosus CBN76663 Thalassiosira pseudonana CCMP1335 XP_002296489 67 59 70 99 Fonticula alba XP_009496667 Neurospora crassa OR74A XP_959535 100 100 Prorocentrum minium CCMP2233 CAMPEP_0177093718 Prorocentrum minium CCMP2233 CAMPEP_0176922572 85 Heterocapsa rotundata SCCAP K-0483 CAMPEP_0168670038 Clupea harengus XP_012678214 100 Salpingoeca rosetta XP_004990633 99 Monosiga brevicollis MX1 XP_001750059 100 Micromonas pusilla CCMP1545 XP_003061831 64 Auxenochlorella protothecoides XP_011398664 100 Helicosporidium sp. ATCC 50920 KDD74633 100 Klebsormidium flaccidum GAQ91993 44 Selaginella moellendorffii XP_002982240 Picea sitchensis ABR16524 Amborella trichopoda XP_006858324 66 100 94 Oryza sativa Japonica Group XP_015639335 100 Nelumbo nucifera XP_010252310 100 64 Beta vulgaris subsp. vulgaris XP_010672596 Daucus carota subsp. sativus KZM85610 98 61Citrus sinensis XP_006488782 66 Camelina sativa XP_010481002 64 99 Cajanus cajan KYP52589 67 Populus trichocarpa XP_002311367 96 Gonium93 pectorale KXZ48195 Chlorella variabilis XP_005843958 Coccomyxa subellipsoidea C-169 XP_005644548 92 Mayorella sp BSH-02190019 CAMPEP_0174242488 98 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168555482 Acytostelium subglobosum LB1 XP_012752469 99 Acytostelium subglobosum LB1 XP_012751200 100 Dictyostelium discoideum AX4 XP_635122 100 98 Stereomyxa ramosa Chinc5 CAMPEP_0174250062 91 Acanthamoeba castellanii str. Neff XP_004352811 Trichosphaerium sp Am-I-7 wt CAMPEP_0168523434 18 99 Pedobacter sp. BAL39 WP_039866815 Paracoccus pantotrophus WP_028709908 Bradyrhizobium sp. BTAi1 WP_012040738 11 98 Phycisphaera mikurensis NBRC 102666 YP_005444358 52 OpitutaceaeTric_XP_001330332 bacterium TAV1 WP_007363100 Desulfovibrio hydrothermalis WP_015337462 100 Kipferlia bialata g5570.t1 97 87 QUERY_g5570.t1_GCS_GCSL 100 Kipferlia bialata c4911_g2_i3 Kipferlia bialata g16410.t1 Kipferlia bialata g13002.t1 100 Halanaerobium praevalens WP_014553185 g5796.t1@anti-oxid@Trx_.com.trim.aln.trimal.treefile Page 1 of 1

0.2 53 Stereum hirsutum FP-91666 SS1 XP_007305505 Sclerotinia sclerotiorum 1980 UF-70 XP_001595273 Oikopleura dioica CBY07962 36 Entamoeba nuttalli P19 XP_008859281 Spironucleus salmonicida EST47112 65 Giardia intestinalis ESU39004 68 70 Giardia intestinalis ESU37167 68 Schizosaccharomyces pombe 972h- NP_592871 64 98 Placozoa sp. H4 AGH70133 64 100 Tetranychus urticae XP_015788270 Kipferlia bialata g5560.t1 94 Strongylocentrotus purpuratus XP_001201177 Latimeria chalumnae XP_006002933 91 Chlorella variabilis XP_005842695 100 Kipferlia bialata g5796.t1 87 QUERY_g5796.t1_anti-oxid_Trx Kipferlia bialata g4470.t1 66 100 Kipferlia bialata g13560.t1 42 Trichomonas vaginalis G3 XP_001325800 93 Schizosaccharomyces japonicus yFS275 XP_002171889 Clonorchis sinensis GAA55399 39 Batrachochytrium dendrobatidis JAM81 XP_006677110 Galdieria sulphuraria XP_005704526 29 27 65 Rhodotorula graminis WP1 KPV76538 Acanthamoeba castellanii str. Neff XP_004349558 50 Mucor ambiguus GAN10163 30 Crassostrea gigas XP_011425198 88 Stereomyxa ramosa Chinc5 CAMPEP_0174276276 88 Acanthamoeba castellanii str. Neff XP_004340525 24 Cyanidioschyzon merolae strain 10D XP_005534838 100 83 Naumovozyma castellii CBS 4309 XP_003676421 66 Millerozyma farinosa CBS 7064 XP_004197278 Dichomitus squalens LYAD-421 SS1 XP_007360787 Lichtheimia ramosa CDS14151 56 58 77 Aspergillus calidoustus CEL03186 63 Pyronema omphalodes CBS 100304 CCX16790 69 Taphrina deformans PYCC 5710 CCG82164 56 Byssochlamys spectabilis No. 5 GAD94099 63 Goniomonas sp m CAMPEP_0114556218 53 82 Monosiga brevicollis MX1 XP_001750589 Aplysia californica XP_005100158 74 Tetraodon nigroviridis CAG05766 65 Hevea brasiliensis AEC03317 42 Chlamydomonas reinhardtii XP_001694158 82 Clupea harengus XP_012676111 Mesobuthus gibbosus CAE54136 52 53 18 Helicosporidium sp. ex Simulium jonesi AAU93947 99 synthetic construct other 2YPM_A 93 synthetic construct other 2YOI_A 36 95 Cristaria plicata AGG17889 Branchiostoma floridae XP_002600511 78 35 50 Phaeodactylum tricornutum CCAP 1055/1 XP_002180660 63 Emiliania huxleyi CCMP1516 XP_005758358 100 Emiliania huxleyi CCMP1516 XP_005774015 Capsaspora owczarzaki ATCC 30864 XP_004363764 62 92 Naegleria gruberi strain NEG-M XP_002680876 Talaromyces islandicus CRG84249 42 91 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168540368 Acanthamoeba castellanii str. Neff XP_004335509 Zygosaccharomyces bailii ISA1307 CDH15198 90 Porphyridium purpureum Porpur_4415.8 Fasciola gigantica AKU75588 Entamoeba histolytica HM-1:IMSS XP_652635 g6006.t1@[email protected] Page 1 of 1

0.2

Ehrlichia ruminantium WP_011155098 Rickettsia raoultii AJQ51833 84 Anaeromyxobacter WP_011419652 2 Phaeospirillum molischianum WP_002727030 Wolbachia WP_006012607 79 18 Vulgatibacter incomptus WP_050726146 80 Sorangium cellulosum WP_061616311 Dongia sp. URHE0060 WP_028098281 Thioalkalivibrio sp. ALR17-21 WP_024329469 Beggiatoa alba WP_002691757 58 endosymbiont of unidentified scaly snail isolate Monju WP_043107631 32 72 Candidatus Thioglobus autotrophica WP_053952068 Moraxella osloensis WP_062330438 Acinetobacter bereziniae WP_058971838 9 88 56 Acinetobacter WP_004812740 71 59 Acinetobacter sp. Ver3 WP_035263622 53 51 35 Acinetobacter equi WP_054582084 96 39Pantholops hodgsonii XP_005978364 Acinetobacter baumannii WP_031955345 Acinetobacter baumannii WP_034688254 18 61 Acinetobacter WP_004786061 87 64 Acinetobacter WP_004925593 57 Acinetobacter WP_004989072 56 marine gamma proteobacterium HTCC2148 WP_007227550 82 Amphritea japonica WP_019622703 61 gamma proteobacterium IMCC1989 WP_009669400 73 65 Endozoicomonas sp. ab112 WP_062269149 Pseudomonas pseudoalcaligenes WP_003448377 43 51synthetic construct other AAT49727 92 95 Pseudomonas WP_003092829 56 Pseudomonas aeruginosa WP_058172517 28 Oblitimonas alkaliphila WP_053101386 30 Pseudomonas thermotolerans WP_017937325 Eikenella corrodens WP_023887211 98 Chitinilyticum aquatile WP_028452109 Rhodocyclaceae bacterium PG1-Ca6 AJP47726 Colwellia piezophila WP_019029442 42 Gilliamella apicola WP_034911174 70 88 Photorhabdus asymbiotica WP_036774753 95 Leminorella grimontii WP_027274097 Escherichia WP_000331708 95 Enterobacteriaceae WP_000331709 75 3 Escherichia coli WP_001686653 46Escherichia coli WP_001493275 14 Cedecea neteri WP_038479961 5321 Mangrovibacter WP_036116369 Escherichia coli WP_050010185 16Shigella sonnei Ss046 YP_311474 49 Echinococcus multilocularis CDS37580 Aplysia californica XP_005096418 92 59 Fistulina hepatica ATCC 64428 KIY49521 42 Candida albicans P78042 KHC68955 34 Galdieria sulphuraria XP_005705575 Nannochloropsis gaditana EWM29688 46 64 Plasmodiophora brassicae CEP00879 75 Bigelowiella natans CCMP2755 Bigna_53822 51 Chrysochromulina sp. CCMP291 KOO27550 100 Oxytricha trifallax EJY82901 82 Stylonychia lemnae CDW74468 54 Pavlovales sp. CCMP2436 Pavl97_c0_seq1 Tric_XP_001580655 100 Kipferlia bialata g6006.t1 QUERY_g6006.t1_ISC_IscU Giar_EFO61914 g6972.t1@[email protected] Page 1 of 1

0.2

Clostridium sp. CAG:967 CCY63908 Opitutaceae bacterium TAV1 WP_007360378 100 Hafnia alvei WP_046359091 Serratia plymuthica WP_020439126 Cedecea neteri WP_038472046 100 81Enterobacter cloacae WP_062934523 Pluralibacter gergoviae WP_048275423 100 Cronobacter sakazakii WP_054625830 65 10076Raoultella terrigena WP_045855119 Raoultella ornithinolytica WP_032687990 80 Klebsiella sp. OBRC7 WP_009652970 100 100[Enterobacter] aerogenes WP_045382747 100Klebsiella pneumoniae WP_032732891 100Klebsiella pneumoniae WP_040245499 5Klebsiella pneumoniae WP_023316285 100Klebsiella pneumoniae WP_023316786 100Enterobacter cloacae WP_048968594 2Klebsiella pneumoniae WP_004183832 71 8413Klebsiella pneumoniae WP_032411415 90 15Klebsiella pneumoniae WP_048333614 Enterobacter cloacae WP_049116251 Aeromonas3 tecta WP_050719504 Methylacidiphilum infernorum V4 YP_001941037 98 Coxiella sp. DG_40 KPJ65787 Arc I group archaeon U1lsi0528_Bin089 KYC50731 Spirochaeta smaragdinae DSM 11293 YP_003802357 41100 Rhodovulum sp. PH10 WP_008386030 50 Arc I group archaeon U1lsi0528_Bin055 KYC53620 64 Commensalibacter intestini WP_040364189 72 Synergistes sp. 3_1_syn1 WP_008710373 81 Arc I group archaeon U1lsi0528_Bin089 KYC52049 51 uncultured bacterium EKD52083 Cyanobium gracile PCC 6307 YP_007047815 84 65 Nostoc sp. PCC 7120 WP_010996075 96 Catabacter hongkongensis WP_046443214 68 Desulfovibrio inopinatus WP_027184542 delta proteobacterium NaphS2 WP_006425210 Planctomycetes bacterium DG_58 KPJ57795 31 74 Paratrimastix pyriformis ABW76110 54 Thermoplasmatales archaeon SG8-52-4 KYK21568 74 bacterium UASB14 GAK50995 92 Syntrophobotulus glycolicus WP_013623407 45 98 Fermentimonas caenicola WP_045088962 87 Staphylococcus sp. CAG:324 CDC71576 47 Arc I group archaeon B03fssc0709_Meth_Bin005 KYC44467 41 Lachnospiraceae bacterium FE2018 WP_035641817 16 29 Kipferlia bialata g8887.t1 27QUERY_g6972.t1_ATP_PFOKipferlia bialata g6972.t1 13Kipferlia bialata c4008_g4_i1 35Kipferlia bialata c4008_g6_i1 30Kipferlia bialata c4008_g2_i1 89 25Kipferlia bialata c4008_g7_i1 Kipferlia bialata c4008_g3_i1 70 Kipferlia bialata c4008_g1_i1 Kipferlia bialata g9091.t1 Kipferlia35 bialata g12658.t1 Kipferlia bialata g3652.t1 99 Kipferlia bialata g9356.t1 59Kipferlia bialata c11319_g1_i1 43 81 Kipferlia bialata g17359.t1 99 Kipferlia bialata g17166.t1 77Kipferlia bialata g15749.t1 Kipferlia bialata g17370.t1 87 Kipferlia bialata c5830_g1_i2 1578Kipferlia bialata g1815.t1 84 Kipferlia bialata g6632.t1 30 Kipferlia bialata g16674.t1 98 3569 Kipferlia bialata g17149.t1 Kipferlia bialata g16706.t1 45Kipferlia bialata c5830_g1_i1 51 100 36 Spironucleus salmonicida AFV80066 Retortamonas sp. Vale ABW76111 Giar_EET01691 100 Mastigamoeba balamuthi AAM53401 Kipferlia bialata c4008_g5_i1 95 100 Kipferlia bialata g5407.t1 86 Kipferlia bialata c4190_g2_i1 17 Kipferlia bialata g2661.t1 Togula jolla CCCM 725 CAMPEP_0170592686 Entamoeba histolytica AAB49653 41 Goniomonas sp m CAMPEP_0114553024 100 Symbiodinium sp. Mp CAMPEP_0169739284 100 Symbiodinium sp. CCMP2430 CAMPEP_0181560754 Symbiodinium sp. CCMP2430 CAMPEP_0181605670 81 100 Guillardia theta CCMP2712 XP_005835303 Cyanophora paradoxa Cpara20400 89 Chlorella variabilis XP_005847443 26 100 Volvox carteri f. nagariensis XP_002947533 100 Entamoeba invadens IP1 XP_004254645 100 Togula jolla CCCM 725 CAMPEP_0170613314 6696 Prorocentrum minium CCMP2233 CAMPEP_0176918648 Prorocentrum minium CCMP2233 CAMPEP_0176891004 100 Perkinsus marinus ATCC 50983 XP_002774160 Perkinsus marinus ATCC 50983 XP_002781654 99 99 Prorocentrum minium CCMP2233 CAMPEP_0177087194 49 68 Prorocentrum minium CCMP2233 CAMPEP_0176853770 64 Prorocentrum minium CCMP2233 CAMPEP_0176855938 99 Perkinsus98 marinus ATCC 50983 XP_002771617 100 Prorocentrum minium CCMP2233 CAMPEP_0176824332 35 Prorocentrum minium CCMP2233 CAMPEP_0176722364 Prorocentrum minium CCMP2233 CAMPEP_0177004560 32 99 Perkinsus33 marinus ATCC 50983 XP_002772895 Thalassiosira pseudonana CCMP1335 XP_002286672 Blastocystis hominis CBK20363 96 Blastocystis hominis XP_012894411 100 Blastocystis hominis XP_012899163 Blastocystis hominis CBK20516 Blastocystis hominis XP_012894564 34 Blastocystis hominis CBK25115 100 Blastocystis sp. ST4 XP_014524937 Blastocystis hominis CBK19555 100 Blastocystis hominis XP_012893603 100Blastocystis hominis CBK23197 Blastocystis hominis CBK20953 Blastocystis sp. ST4 XP_014527203 100Blastocystis hominis XP_012897901 31 100 Blastocystis hominis CBK23853 100 Blastocystis hominis XP_012893814 45 100Blastocystis hominis CBK19766 99 Trichosphaerium60 sp Am-I-7 wt CAMPEP_0168531048 Scrippsiella trochoidea CCMP3099 CAMPEP_0115457366 Scrippsiella trochoidea CCMP3099 CAMPEP_0115677502 100 Scrippsiella trochoidea CCMP3099 CAMPEP_0115282244 97 Eutreptiella100 gymnastica NIES-381 CAMPEP_0113752326 Stereomyxa ramosa Chinc5 CAMPEP_0174261428 100 Sawyeria marylandensis ABU95423 Acanthamoeba castellanii str. Neff XP_004336994 81 Tric_AAA85495 81 Bacteroides sp. CAG:927 CCX47715 g7565.t1@[email protected] Page 1 of 1

0.2 85 Candidatus Caldiarchaeum subterraneum WP_020864117 Microcoleus sp. PCC 7113 YP_007123076 96 Cenarchaeum symbiosum A YP_875058 84 Hydrogenobacter thermophilus TK-6 YP_003431869 Aciduliprofundum boonei T469 YP_003483358 Methanopyrus kandleri AV19 NP_614016 95 84 Methanobacterium sp. AL-21 WP_013644230 100 Kipferlia bialata g7565.t1 80 QUERY_g7565.t1_ATP_SCSa 100 Histomonas meleagridis ACI16481 Coptotermes formosanus AGM32445 Trichomonas vaginalis AAC48339 86 Plasmodiophora 86brassicae RhEST0037790 17 100 Scrippsiella trochoidea CCMP3099 CAMPEP_0115439640 100 Scrippsiella trochoidea CCMP3099 CAMPEP_0115679368 Togula jolla CCCM 725 CAMPEP_0170651478 99 Phytomonas sp. isolate EM1 CCW64321 100 Leishmania infantum JPCM5 XP_001466253 100 Leishmania braziliensis MHOM/BR/75/M2904 XP_001565674 100 Trypanosoma cruzi strain CL Brener XP_820834 98 Neobodo designis CCAP 1951/1 CAMPEP_0174859558 42 Bodo saltans BS34915 100 Euglena gracilis Euggra783_c0_seq1 Eutreptiella gymnastica NIES-381 CAMPEP_0113746384 100100Perkinsus marinus ATCC 50983 XP_002768651 100 Perkinsus marinus ATCC 50983 XP_002772148 3065 Perkinsus marinus ATCC 50983 XP_002781462 Perkinsus marinus ATCC 50983 XP_002779820 94 Blastocystis sp. NandII ABW76150 58 Blastocystis hominis XP_012897605 100 Blastocystis hominis CBK23557 99 Blastocystis hominis CBK19683 39Blastocystis hominis XP_012899443 100Blastocystis hominis XP_012893731 Blastocystis hominis CBK25395 100 Plasmodium fragile XP_012337010 83 Plasmodium reichenowi XP_012763495 75 Plasmodium chabaudi chabaudi XP_740099 100 Babesia bigemina XP_012768969 Theileria equi XP_004831195 100 Vitrella brassicaformis CCMP3155 CEM02135 83 Eimeria mitis XP_013352256 Gregarina niphandrodes XP_011129086 81 100Neospora caninum Liverpool CEL68504 91 Neospora caninum Liverpool XP_003881225 97 Hammondia hammondi XP_008889195 100 Toxoplasma gondii ME49 XP_002368454 100 100 Stylonychia lemnae CDW71518 Oxytricha trifallax EJY75338 Sawyeria marylandensis ABU95432 53Paramecium tetraurelia strain d4-2 XP_001425910 91 100 Paramecium tetraurelia strain d4-2 XP_001455642 91 Paramecium tetraurelia strain d4-2 XP_001452638 Pseudocohnilembus persalinus KRX07276 52 99 Ichthyophthirius multifiliis XP_004029838 97 Tetrahymena thermophila SB210 XP_001007751 99 Goniomonas sp m CAMPEP_0114548982 Phaeodactylum tricornutum CCAP 1055/1 XP_002180696 82 7296 Aphanomyces invadans XP_008871215 Saprolegnia parasitica CBS 223.65 XP_012207777 100 Albugo candida CCI47059 62 Phytophthora sojae XP_009524111 69 97 Plasmopara halstedii CEG46057 94 100 Phytophthora parasitica INRA-310 XP_008905429 91Gymnodinium catenatum GC744 CAMPEP_0117571960 Aureococcus anophagefferens Auran_28269 79 Ectocarpus siliculosus CBJ28950 Reticulomyxa filosa ETO36756 Guillardia theta CCMP2712 XP_005837665 99 64 Galdieria sulphuraria XP_005708256 95 Mayorella sp BSH-02190019 CAMPEP_0174243356 44 93 Acanthamoeba castellanii str. Neff XP_004352450 52 64 Stereomyxa ramosa Chinc5 CAMPEP_0174251334 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168547830 93100Dictyostelium fasciculatum XP_004351803 97 Acytostelium subglobosum LB1 XP_012749036 Polysphondylium pallidum PN500 EFA80958 100 100 Dictyostelium discoideum AX4 XP_636263 99 Monomorium pharaonis XP_012528802 75 Magnaporthiopsis poae ATCC 64411 KLU85715 90 Stegodyphus mimosarum KFM71011 98 Opisthocomus hoazin KFR10822 35 Caenorhabditis briggsae XP_002642901 63 Trichoplax adhaerens XP_002117441 97 Ustilago maydis 521 XP_757819 Monosiga brevicollis MX1 XP_001744918 72 86 Salpingoeca rosetta XP_004995026 53 Corallomyxa tenera Corten609_c0_seq1 Trichosphaerium sp Am-I-7 wt CAMPEP_0168515034 95 Bigelowiella natans CCMP2755 Bigna_53292 Cyanophora paradoxa Cpara29230 57 Ostreococcus tauri XP_003078495 Klebsormidium flaccidum GAQ81611 98 Physcomitrella patens XP_001762925 88 Hordeum vulgare subsp. vulgare BAJ94034 100 Eucalyptus grandis XP_010069514 100 Eucalyptus grandis XP_010031908 90 99 42Nelumbo nucifera XP_010241497 Brassica oleracea var. oleracea XP_013595305 936886Arachis duranensis XP_015966208 97 Daucus carota subsp. sativus KZN09933 87 Zostera marina KMZ72542 74 Capsicum annuum NP_001311675 1585Jatropha curcas XP_012079367 97 86Populus trichocarpa XP_002318347 68Populus trichocarpa XP_006376608 5716Vitis vinifera XP_002271746 Glycine max NP_001241205 Coccomyxa subellipsoidea C-169 XP_005643816 Bathycoccus prasinos XP_007512066 79 Helicosporidium sp. ATCC 50920 KDD74604 93 77 Chlamydomonas reinhardtii XP_001693109 78 Chlorella variabilis XP_005843440 97 Pavlovales sp. CCMP2436 Pavl539_c0_seq1 Emiliania huxleyi CCMP1516 XP_005790040 100 Isochrysis aff. galbana Isogal36556_c0_seq1 98 uncultured bacterium EB000_36F02 ADI19715 64 Thermopetrobacter sp. TC1 WP_038035751 Xanthomonas WP_011038208 55 Chromobacterium WP_043592732 100 Pseudoalteromonas luteoviolacea WP_005493862 66 99 Catenovulum agarivorans WP_035012767 100 Marinobacterium stanieri WP_010324266 98 Nematostella vectensis XP_001617908 39 99 100 Legionella pneumophila str. Corby YP_001252016 Parachlamydia acanthamoebae UV-7 YP_004653287 Zavarzinella formosa WP_020468237 96 Sporosarcina koreensis WP_060209089 Kipferlia bialata g12539.t1 83 57 Denitrovibrio acetiphilus DSM 12809 YP_003505062 97 Granulicella tundricola WP_013581550 Sphingobacterium sp. 21 WP_013665693 88 84 Roseiflexus sp. RS-1 YP_001277561 89 Ktedonobacter racemifer WP_007909034 85 Bacteroides thetaiotaomicron VPI-5482 NP_809700 59 Natrialba magadii ATCC 43099 YP_003478883 Amycolatopsis mediterranei S699 YP_005528770 Archaeoglobus fulgidus DSM 4304 NP_071010 g7998.t1@[email protected] Page 1 of 1

0.5

Synergistes sp. 3_1_syn1 WP_008708829 SAR202 cluster bacterium SCGC AAA240-N13 WP_017953863 Sphaerobacter thermophilus DSM 20745 YP_003319907 87 Burkholderia multivorans ATCC 17616 YP_001580548 41 98 Cyanothece sp. PCC 7822 YP_003888690 92 Synechococcus sp. WH 5701 WP_006170042 92 100 Synechococcus sp. CB0101 WP_010307173 Sebaldella termitidis ATCC 33386 YP_003310876 51 Methanobacterium sp. AL-21 WP_013646079 bacterium L21-Spi-D4 WP_057951989 100 Kipferlia bialata g7998.t1 50 QUERY_g7998.t1_ATP_hydrogenase 58 Nannochloropsis gaditana CCMP526 XP_005854537 Naegleria gruberi strain NEG-M XP_002674266 41 95 Sawyeria marylandensis ABU95425 100 Psalteriomonas lanterna ACZ97599 52 Monoraphidium neglectum XP_013901261 93 Chlorella variabilis XP_005848612 95 Neocallimastix frontalis AFJ73534 57 Cyanophora paradoxa Cpara09130 45 Volvox carteri f. nagariensis XP_002948487 74 Stereomyxa ramosa Chinc5 CAMPEP_0174270240 Hydrogenovibrio marinus WP_029909256 93 89 100 Thalassiosira pseudonana CCMP1335 XP_002295160 51 Salpingoeca rosetta XP_004994885 Corallomyxa tenera Corten33801_c0_seq1 91 98 Porphyridium purpureum Porpur_2663.1 Thermotoga maritima MSB8 NP_228016 75 Kipferlia bialata g166.t1 100 Kipferlia bialata g1491.t1 Kipferlia bialata g16293.t1 100 Kipferlia bialata g1390.t1 17 30Kipferlia bialata c3397_g1_i1 Kipferlia bialata g11856.t1 100 Kipferlia bialata c4020_g3_i1 83 Blastocystis sp. ST4 XP_014527296 Blastocystis sp. NandII ACD10930 87 100 99Blastocystis hominis XP_012899174 100Blastocystis hominis CBK25126 Cyanophora paradoxa Cpara20370 100 99 Stereomyxa ramosa Chinc5 CAMPEP_0174263482 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168567040 72 Dictyostelium purpureum XP_003284570 97 Coccomyxa subellipsoidea C-169 XP_005643907 79 76 Arabidopsis lyrata subsp. lyrata XP_002868134 76 Ostreococcus lucimarinus CCE9901 XP_001416706 84 65 Monosiga brevicollis MX1 XP_001743410 98 Rattus norvegicus NP_001013201 98 Histomonas meleagridis ACI16483 97 Trichomonas vaginalis AAC47159 Pseudotrichonympha grassii BAF82036 100 Tric_XP_001305709 Pleomorphomonas koreensis WP_053239729 47 [Clostridium] sordellii CEN95816 Clostridium WP_010296878 75 96 Pseudotrichonympha grassii BAF82035 100 Trichomonas vaginalis G3 XP_001328981 54 Hydrogenobacter thermophilus TK-6 YP_003431786 37 53 Entamoeba invadens IP1 XP_004259337 100 Clostridium sp. KLE 1755 WP_021636344 Parcubacteria group bacterium GW2011_GWC2_42_12 KKS35389 57 Kipferlia bialata g3616.t1 Retortamonas sp. Vale ABW76107 80 81 99 Spironucleus salmonicida AFV80058 86 Trepomonas sp. PC1 AGV05436 97 Giardia intestinalis assemblage B KWX12496 99 Giardia intestinalis ESU38027 79 100 99 Spironucleus salmonicida EST48803 Trepomonas sp. PC1 AGV05432 99 Spironucleus salmonicida AFV80056 96 Retortamonas sp. Vale ABW76106 100 Entamoeba histolytica HM-1:IMSS XP_656685 [Eubacterium] siraeum V10Sc8a CBL34874 72 Desulfurobacterium sp. TC5-1 WP_022847517 Ignavibacterium album WP_014559401 97 Clostridium botulinum WP_035791323 100 Clostridium sartagoforme WP_016206989 synthetic construct other AIR95607 2953 25synthetic construct other AIR95591 synthetic construct other AIR95597 98 84 Clostridium tyrobutyricum WP_058953412 84 82 Clostridium acetobutylicum WP_010963357 95 80synthetic construct other AIR95602 synthetic construct other AIR95595 candidate division TM6 bacterium GW2011_GWA2_36_9 KKQ18915 Halonatronum saccharophilum WP_027338952 90 Clostridium cadaveris WP_027637394 70 Sporomusa ovata DSM 2662 EQB28956 Gracilibacter sp. BRH_c7a KUO63351 58 91 Verrucomicrobia bacterium L21-Fru-AB WP_052881068 Desulfotomaculum sp. 46_296 KUK52976 65 Dehalococcoides mccartyi WP_012984090 100 100 uncultured archaeon CBH37505 72 Dethiobacter alkaliphilus WP_008516702 62 Syntrophomonas wolfei WP_011640436 64 Neobodo designis CCAP 1951/1 CAMPEP_0174867010 g8060.t1@[email protected] Page 1 of 1

0.2

Clostridium baratii WP_054199361

Anaerococcus prevotii WP_015777235 100

Blautia hydrogenotrophica WP_040350420 80

95 Clostridium bolteae CAG:59 CCX96673 97

94 Brachyspira alvinipulli WP_028330065

64 93 Bacteroides uniformis WP_016273576

98 Lachnospiraceae bacterium M18-1 WP_016295367

Clostridium perfringens WP_061429259

candidate division TA06 bacterium 34_109 KUK86119

Marinilabilia salmonicolor WP_010662748

candidate division Hyd24-12 bacterium Vib_1 KZD15185

Kipferlia bialata g8060.t1 96

100 QUERY_g8060.t1_ATP_HydE 61

98 Kipferlia bialata c1578_g1_i1

Trepomonas sp. PC1 AGV05424 68 100 Spironucleus salmonicida AFV80053

82 Trichomonas vaginalis G3 XP_001322256

Paratrimastix pyriformis AGH33865

69 Naegleria gruberi strain NEG-M XP_002671091

Cyanophora paradoxa Cpara24908

99 Mastigamoeba balamuthi AGA37362 90

Nannochloropsis gaditana EWM24357 44 35

35 Tetraselmis sp. GSL018 AHH85810 94 38 Porphyridium purpureum Porpur_2123.3

53 Acanthamoeba castellanii str. Neff XP_004344718

Chlamydiae bacterium SM23_39 KPK32772

Syntrophomonas zehnderi WP_046500138 g8234.t1@[email protected] Page 1 of 1

0.2

Carboxydothermus hydrogenoformans WP_011344455

Clostridium sp. CAG:167 CCZ91164

Clostridium botulinum WP_003400997

97 Lachnospiraceae bacterium 5_1_63FAA WP_009264064

45 Clostridium sp. CAG:138 CDA51812 96

Desulfosporosinus sp. OT WP_009613219 39 83

Desulfosporosinus orientis WP_014186017 95 82

Blautia sp. CAG:257 CDA04994

Mogibacterium WP_036381375

Kipferlia bialata g8234.t1 34 100 100 Kipferlia bialata c10235_g1_i1

QUERY_g8234.t1_ATP_HydF

48 Prolixibacter bellariivorans WP_025863371

Chlamydiae bacterium SM23_39 KPK32771

51 Paratrimastix pyriformis AGH33866 48 82 62 Nannochloropsis gaditana CCMP526 XP_005855690

76

Naegleria gruberi strain NEG-M XP_002679321

76 79 Acanthamoeba castellanii str. Neff XP_004344717

Porphyridium purpureum Porpur_3384.19

Tric_XP_001309182

Trepomonas sp. PC1 AGV05425

100 Spironucleus salmonicida AFV80054 g8254.t1@[email protected] Page 1 of 1

0.2

Bradyrhizobium sp. BTAi1 YP_001240998 Ktedonobacter racemifer WP_007923416 100 Kipferlia bialata g8254.t1 100 QUERY_g8254.t1_ATP_ACS 100 Trepomonas sp. PC1 AGV05441 100 Spironucleus barkhanus AAM94652 65 98 Giardia intestinalis assemblage B KWX15836 71 Lokiarchaeum sp. GC14_75 KKK41115 Carboxydothermus hydrogenoformans WP_011343212 87 Candidatus Thorarchaeota archaeon SMTZ1-45 KXH70921 69 Methanobrevibacter millerae WP_058739602 100 Peptococcaceae bacterium BRH_c8a KJS12369 Candidatus Bathyarchaeota archaeon B26-2 KYH40071 86 Euryarchaeota archaeon 55_53 KUK04788 Acetothermia bacterium 64_32 KUK27386 candidate divison MSBL1 archaeon SCGC-AAA259E22 KXA93869 82 100 Halorhabdus utahensis WP_015790014 100 Natrinema pallidum WP_006186605 100 100 Halobacteriaceae archaeon SB9 WP_058581392 97 Haloferax sp. Q22 WP_058828184 96 Halonotius sp. J07HN4 ERH06972 99 100 uncultured archaeon A07HN63 ESS10027 100 100Halonotius sp. J07HN6 ERH02410 100 uncultured archaeon A07HR67 ESS03736 67 Haloquadratum walsbyi J07HQW2 ERG94344 85 100 Halorubrum sp. 5 WP_053771928 Archaeoglobus fulgidus WP_048095590 Thermodesulfovibrio WP_012545569 88 Parcubacteria group bacterium GW2011_GWC2_39_14 KKR04361 91 Methanobacterium lacus WP_013645228 88 Methanotorris formicicus WP_007044454 51 Methanomassiliicoccales archaeon RumEn M1 KQM12761 66 Thaumarchaeota archaeon N4 WP_048194424 Thermofilum sp. 1910b WP_020962532 59 Thermoplasmatales archaeon DG-70 KYK37660 100 98 Thermanaerothrix daxensis KPL84829 100 Aciduliprofundum boonei T469 YP_003482693 82 84 Candidatus Pacebacteria bacterium GW2011_GWF1_36_5 KKQ10238 Desulfatitalea tepidiphila WP_054032596 73 Bacteroides thetaiotaomicron VPI-5482 NP_811836 Sutterella parvirubra WP_008542307 93 Amycolatopsis mediterranei S699 YP_006548873 96 100 Archaeoglobus fulgidus DSM 4304 NP_070340 98 Candidatus Nitrospira defluvii WP_013250359 77 Anaerolinea thermophila UNI-1 YP_004174188 100 Thiobacillus denitrificans WP_011312536 Bradyrhizobium sp. BTAi1 WP_012045664 Escherichia coli str. K-12 substr. MG1655 NP_417079 100 64Amphidinium carterae CCMP1314 CAMPEP_0176664694 100 Amphidinium carterae CCMP1314 CAMPEP_0176655988 100 Amphidinium carterae CCMP1314 CAMPEP_0176610904 76 Persephonella sp. KM09-Lau-8 WP_029521917 100 Desulfobacula sp. TS WP_031452015 98 Draconibacterium sediminis WP_045033154 uncultured archaeon CAI64211 100 Dehalococcoides mccartyi WP_058316855 96 Peptococcaceae bacterium SCADC1_2_3 KFD41576 74 100 100 Hadesarchaea archaeon YNP_45 KUO39680 97 Candidatus Methanoperedens nitroreducens KCZ70712 100 miscellaneous Crenarchaeota group-15 archaeon DG-45 KON30995 100 Candidatus Bathyarchaeota archaeon BA2 KPV63469 Candidatus Cloacimonas sp. SDB KQC03587 86 100 Entamoeba histolytica HM-1:IMSS XP_656290 70 Pyrodinium bahamense pbaha01 CAMPEP_0179078296 Methylacidiphilum infernorum V4 YP_001938680 95 Vitrella brassicaformis CCMP3155 CEL97119 100 Candidatus Protochlamydia sp. R18 WP_042281785 98 uncultured bacterium EKE08653 96 28 Thermobaculum terrenum WP_012874842 29 Nodularia spumigena CENA596 KZL48204 Candidatus Koribacter versatilis WP_011523808 90 98 65 Desulfobacterales bacterium SG8_35_2 KPK28946 70 Thalassiosira pseudonana CCMP1335 XP_002294673 Archangium gephyra WP_047861226 Caldilinea aerophila DSM 14535 = NBRC 104270 YP_005440863 75 9682 43 Cyanophora paradoxa Cpara15250 68 Cyanidioschyzon merolae strain 10D XP_005537438 Blastocystis hominis CBK25099 100Symbiodinium sp. Mp CAMPEP_0169854300 45 Symbiodinium sp. Mp CAMPEP_0169633678 100 Symbiodinium sp. CCMP2430 CAMPEP_0181593022 98 Spirochaeta smaragdinae DSM 11293 YP_003805024 Candidatus Caldiarchaeum subterraneum YP_008796565 77 Methanopyrus kandleri AV19 NP_614529 Thermococcus kodakarensis KOD1 YP_183357 94 Methanosarcina acetivorans C2A NP_618061 61 Blastocystis hominis CBK21741 g8321.t1@[email protected] Page 1 of 1

0.2

56 Ktedonobacter racemifer WP_007919378 37 Selenomonas sputigena ATCC 35185 YP_004414462 36 Spirochaeta smaragdinae DSM 11293 YP_003804094 65 Thermotoga maritima MSB8 YP_007977221 Hydrogenobacter thermophilus TK-6 YP_005512503 Methanosarcina acetivorans C2A NP_616304 99 34 Clostridium sp. KLE 1755 WP_021639445 36 Eggerthella sp. YY7918 WP_013980227 7094 Verrucomicrobiae bacterium DG1235 WP_008105031 Methanocaldococcus jannaschii DSM 2661 NP_248544 Aciduliprofundum boonei T469 YP_003483648 97 Methanobacterium sp. AL-21 YP_004291631 59 98 Thermococcus kodakarensis KOD1 YP_184513 Deinococcus gobiensis I-0 YP_006261724 100 QUERY_g8321.t1_anti-oxid_TrxRKipferlia bialata g8321.t1 100 Kipferlia bialata c11353_g1_i1 86 Giardia lamblia P15 EFO64859 Spironucleus salmonicida AFV80083 100 Candidatus Babela massiliensis WP_023791211 68 Nitrospina sp. SCGC_AAA799_C22 WP_048493307 Nostoc sp. PCC 7120 WP_010996362 Cyanophora paradoxa Cpara14153 100 Calothrix sp. 336/3 WP_035149675 64 95 Synechococcus sp. WH 5701 WP_006171763 84 Ectocarpus siliculosus CBN78458 84 Brassica rapa XP_009142765 87 Bigelowiella natans CCMP2755 Bigna_92474 98 Ostreococcus lucimarinus CCE9901 XP_001422184 84 Chlorella variabilis XP_005847378 99 Chlamydomonas reinhardtii XP_001689807 Acanthamoeba95 castellanii str. Neff XP_004351681 78 Leptospira santarosai WP_004481526 Leptonema illini WP_002769248 62 72 Spizellomyces punctatus DAOM BR117 XP_016608363 97 Phycomyces blakesleeanus NRRL 1555(-) OAD79195 54 Gonapodya prolifera JEL478 KXS19638 63 Fonticula alba XP_009493244 87 Spirochaeta africana WP_014454806 Lentisphaera araneosa WP_007280382 37 99 Euglena gracilis BAO53980 97 Euglena gracilis Euggra2841_c0_seq1 92 67 100100 Dictyostelium fasciculatum XP_004366633 Acytostelium subglobosum LB1 XP_012749342 Polysphondylium pallidum PN500 EFA76581 71 100 100 Dictyostelium purpureum XP_003288816 65 100 Entamoeba histolytica HM-1:IMSS XP_655748 Entamoeba dispar SAW760 XP_001739375 100 Entamoeba invadens IP1 XP_004259956 100 Neochlamydia sp. TUME1 WP_039385274 91 Simkania negevensis WP_013943178 Acytostelium subglobosum LB1 XP_012750090 Chlamydia trachomatis WP_032139854 64 Myxococcales bacterium SG8_38 KPK16880 74 81 Porphyridium purpureum Porpur_3471.4 Chondrus crispus XP_005711773 Galdieria sulphuraria XP_005705270 53 97 93 Chlamydomonas reinhardtii XP_001699827 80 Citrus sinensis KDO71600 94 Coccomyxa subellipsoidea C-169 XP_005649691 79 98 Selaginella moellendorffii XP_002965676 91 Chlorella variabilis XP_005850010 83 Volvox carteri f. nagariensis XP_002956971 80 Cyanidioschyzon merolae strain 10D XP_005536436 95 Salpingoeca rosetta XP_004994306 55 Monosiga brevicollis MX1 XP_001748467 100 Hassallia byssoidea WP_039750997 Rhodopirellula baltica SH 1 NP_867705 85 Trichomonas vaginalis G3 XP_001311468 99 Trichomonas vaginalis G3 XP_001328714 100 Histomonas meleagridis CAQ86691 46 65 Tric_XP_001316923 93 Bdellovibrio96 bacteriovorus WP_011162962 candidate division WS6 bacterium OLB20 KXK25723 Trichosphaerium sp Am-I-7 wt CAMPEP_0168531290 95 Opitutaceae bacterium TAV1 WP_007358887 52 50 Stereomyxa ramosa Chinc5 CAMPEP_0174257112 99 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168557582 97 Ilyobacter polytropus WP_013388892 SAR324 cluster bacterium SCGC AAA240-J09 WP_040921460 Caulobacter henricii WP_035088342 Magnetococcus marinus WP_011712110 82 100 Acidithiobacillus thiooxidans WP_031573736 Francisella tularensis WP_014547836 Legionella pneumophila WP_062728562 87 Coxiella burnetii WP_040947892 93 100 Rudaea cellulosilytica WP_018974606 Stenotrophomonas koreensis WP_057665408 60 93 92 Mizugakiibacter sediminis WP_062537583 100 Luteibacter WP_036112649 54 99 Dyella ginsengisoli WP_017461089 99 Rhodanobacter sp. FW104-R8 KZC15342 98 Rhodanobacter fulvus WP_007079738 100 99 Frateuria sp. Soil773 WP_056005730 55 97Dorcoceras hygrometricum KZV14897 100 68Pandoraea faecigallinarum WP_047907349 64 Pseudogulbenkiania ferrooxidans WP_021475964 Uliginosibacterium gangwonense WP_018607537 100 Ricinus communis XP_002534945 99 100 Vibrio campbellii ATCC BAA-1116 YP_008524889 Mariprofundus ferrooxydans WP_018293975 Melioribacter roseus WP_014855086 47 Anaeromyxobacter sp. K WP_012528019 81 Bacteriovorax sp. Seq25_V WP_021274792 71 Vulgatibacter incomptus WP_050726360 60 uncultured bacterium EKD50635 67 Cystobacter fuscus WP_002643404 Herbidospora cretacea WP_061290543 4272 Candidatus Nitrospira inopinata WP_062486418 Paenibacillus macerans WP_036619700 Natrialba magadii ATCC 43099 YP_003481751 66 100 Ectocarpus siliculosus CBN74530 45 Scrippsiella trochoidea CCMP3099 CAMPEP_0115720350 Scrippsiella trochoidea CCMP3099 CAMPEP_0115389282 100 Scrippsiella trochoidea CCMP3099 CAMPEP_0115646150 71 100 Aureococcus anophagefferens Auran_23346 Gymnodinium catenatum GC744 CAMPEP_0117558062 100 Emiliania huxleyi CCMP1516 XP_005781793 71 Isochrysis aff. galbana Isogal36615_c1_seq1 Prorocentrum minium CCMP2233 CAMPEP_0176932034 100 Karlodinium micrum CCMP2283 CAMPEP_0169164248 79 100 Karlodinium micrum CCMP2283 CAMPEP_0169335478 65 100 Aureococcus anophagefferens Auran_13221 100 100 Prorocentrum minium CCMP2233 CAMPEP_0177080566 32 Prorocentrum minium CCMP2233 CAMPEP_0176879774 Peridinium aciculiferum PAER-2 CAMPEP_0183603408 71 100 Phaeodactylum tricornutum CCAP 1055/1 XP_002182008 Pavlovales sp. CCMP2436 Pavl4393_c0_seq2 68 Guillardia theta CCMP2712 XP_005830962 Acidimicrobium100 ferrooxidans DSM 10331 YP_003108678 96 Candidatus Micrarchaeum acidiphilum ARMAN-2 EET90140 Thermaerobacter marianensis WP_013494541 Candidatus Caldiarchaeum subterraneum WP_020864455 61 Candidatus Nitrososphaera gargensis Ga9.2 YP_006862760 96 Roseiflexus sp. RS-1 YP_001274488 g8910.t1@ATP@ASCT_C.com.trim.aln.trimal.treefile Page 1 of 1

0.5 Chlorella variabilis XP_005848746

Roseiflexus sp. RS-1 YP_001275270 86 Sphaerobacter thermophilus DSM 20745 YP_003319976 83 Spirosoma linguale DSM 74 YP_003386738 100 Kipferlia bialata g8910.t1 QUERY_g8910.t1_ATP_ASCT_C 100 Peridinium aciculiferum PAER-2 CAMPEP_0183411540 Peridinium aciculiferum PAER-2 CAMPEP_0183536258 Blastocystis sp. ST4 XP_014529058 100 98 Blastocystis hominis CBK20133

100Blastocystis hominis XP_012896210 Blastocystis hominis XP_012894181 Blastocystis hominis CBK22162

Geoalkalibacter subterraneus WP_040200511 Schizosaccharomyces cryophilus OY26 XP_013021534 86 65 Naegleria gruberi strain NEG-M XP_002679707 100 Acytostelium subglobosum LB1 XP_012758141 74 Polysphondylium pallidum PN500 EFA75097 90 100 Dictyostelium purpureum XP_003285106 44 Exidia glandulosa HHB12029 KZV96834 81 Cyberlindnera jadinii BAN45665 Pyronema omphalodes CBS 100304 CCX05364 93 Rosellinia necatrix GAP87907 98 Xylona heveae TC161 KZF23505 98 Blastomyces dermatitidis ER-3 EEQ84670 100 100 Talaromyces verruculosus KUL86427 100 Aspergillus kawachii IFO 4308 GAA89234 519432 Aspergillus nidulans FGSC A4 XP_659151 Aspergillus nomius NRRL 13137 XP_015401689 99 Aspergillus fumigatus var. RP-2014 KEY82814 73 55 38 Aspergillus ruber CBS 135680 EYE91819 25 Byssochlamys spectabilis No. 5 GAD94241 49Penicillium brasilianum CEJ57739 Plutella xylostella XP_011567019 33 87 85 64 100 Penicillium rubens Wisconsin 54-1255 XP_002557199 Macrophomina phaseolina MS6 EKG14275 Cyphellophora europaea CBS 101466 XP_008711695 99 Exophiala spinifera XP_016240231 52Fonsecaea monophora OAG39948 92 86 8952Cladophialophora carrionii CBS 160.54 XP_008728918 45 Capronia semi-immersa KIW67298 79 Exophiala aquamarina CBS 119918 XP_013261818 Coniosporium apollinis CBS 100218 XP_007778998 68 44 Verruconis gallopava XP_016214309 Pyrenophora teres f. teres 0-1 XP_003300329 96 Rhizopus microsporus CEJ02278 73 Rhizophagus irregularis DAOM 181602 ESA18715 98 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168557594 Tric_XP_001330176 89 Stigmatella aurantiaca DW4/3-1 YP_003951353 Phascolarctobacterium succinatutens CAG:287 CDD12955 Synergistales bacterium 54_24 KUK38960

Pseudomonas amygdali WP_029240829 91 Verrucomicrobiae bacterium DG1235 WP_008103577 Comamonas WP_039049343

Comamonas WP_059440139 100 99 Brachymonas chironomi WP_026320515 100 Entamoeba invadens IP1 XP_004258381 100 Burkholderia multivorans ATCC 17616 YP_001584437 g9549.t1@[email protected] Page 1 of 1

0.2 Acanthamoeba castellanii str. Neff XP_004339140 39 82 Dictyostelium lacteum KYR00979 Ostreococcus tauri XP_003081249 45 Thecamonas trahens ATCC 50062 XP_013760877 50 Mayorella sp BSH-02190019 CAMPEP_0174235664 89 Drosophila melanogaster NP_001097616 59 Metaseiulus occidentalis XP_003739036 Haemonchus contortus CDJ88136 55 58 Naegleria gruberi strain NEG-M XP_002680110 Trichuris suis KFD52275 97 Helobdella robusta XP_009025342 84 Ciona intestinalis XP_002123428 85 Strongylocentrotus purpuratus XP_796457 92 Branchiostoma floridae XP_002612056 88 Sparus aurata AGU38789 Rana catesbeiana ACO51809 97 Latimeria chalumnae XP_006001117 99 Amazona aestiva KQK75641 85 Monodelphis domestica XP_001380062 98 Dasypus novemcinctus XP_004461765 54 70 59 Ailuropoda melanoleuca EFB25742 Oryctolagus cuniculus XP_008272846 100 60 Microcebus murinus XP_012619272 22 Condylura cristata XP_004690920 65 Orycteropus afer afer XP_007946181 Callithrix jacchus XP_002743684 97 Pan paniscus XP_008971824 97 Nomascus leucogenys XP_003258081 85 Rhinopithecus roxellana XP_010357506 17 Papio anubis XP_003905420 56 37 87Homo sapiens NP_741999 Capsaspora owczarzaki ATCC 30864 XP_004348834 49 Salpingoeca rosetta XP_004990839 82 Volvox carteri f. nagariensis XP_002948275 40 Guillardia theta CCMP2712 XP_005833979 42 Vitis vinifera XP_002270858 49 Plasmodiophora brassicae CEP02045 93 Corallomyxa tenera Corten19702_c0_seq1 Magnetospira sp. QH-2 WP_046023302 44 Candidatus Methylopumilus planktonicus WP_046488468 96 Rheinheimera sp. A13L WP_008900495 Pandoraea vervacti WP_044457929 59 97 99 Methylovorus glucosetrophus SIP3-4 ACT51094 71 Methylophilus sp. OH31 WP_024931022 100 Methylotenera sp. 1P/1 WP_020182525 85 99 79 100 Pantholops hodgsonii XP_005974535 Sideroxydans lithotrophicus WP_013030378 Thermithiobacillus tepidarius WP_051180733 Tric_XP_001308237 39 Reticulomyxa filosa ETO01395 Calocera viscosa TUFC12733 KZO93500 75 Perkinsus marinus ATCC 50983 XP_002782631 100 Kipferlia bialata g9549.t1 64 QUERY_g9549.t1_ISC_HscB 65 Blastocystis sp. ST4 XP_014527528 34 77 Giar_EFO65039 Ectocarpus siliculosus CBJ29355 Nitrospira moscoviensis WP_053381513 100 Aphanomyces astaci XP_009836136 39 99 Saprolegnia diclina VS20 XP_008617831 Phytophthora parasitica ETL93208 70 100 Plasmopara halstedii CEG48525 Nannochloropsis gaditana EWM26251 g10020.t1@[email protected] Page 1 of 1

0.2 Entamoeba histolytica HM-1:IMSS XP_648117 100 CorallomyxaQUERY_g10020.t1_ISC_Hsp70 tenera Corten1862_c0_seq1 11 Kipferlia bialata c11432_g1_i1 Eutreptiella gymnastica NIES-381 CAMPEP_0113747192 100 Neobodo designis CCAP 1951/1 CAMPEP_0174877776 Trypanosoma cruzi strain CL Brener XP_806221 99 Leishmania braziliensis MHOM/BR/75/M2904 XP_001566868 100 Leishmania infantum JPCM5 XP_001467094 70 Euglena100 gracilis Euggra812_c0_seq1 Eutreptiella gymnastica NIES-381 CAMPEP_0113756134 14100 Naegleria gruberi strain NEG-M XP_002681196 Reticulomyxa filosa ETO10372 58 Wolbachia pipientis WP_006013401 66 Skermanella stibiiresistens WP_037451733 Devosia sp. Root635 WP_056231077 Bartonella bovis WP_010701881 79 Kaistia granuli WP_018182249 Xanthobacter sp. 126 WP_024281253 100 Blastochloris viridis WP_055038256 100 Variibacter gotjawalensis BAT61761 98 Rhodoplanes sp. Z2-YC6860 AMN38759 85 Afipia clevelandensis WP_002713856 100 91Bradyrhizobium retamae WP_057844820 11 100 Bradyrhizobium sp. LTSP849 WP_045012881 100 100 Bradyrhizobium elkanii WP_028164804 7197Rhodopseudomonas sp. AAP120 WP_054160878 62 87 Nitrobacter winogradskyi WP_011313533 92 91 Afipia sp. P52-10 WP_034465207 40Bradyrhizobium sp. STM 3843 WP_008968887 7057Rhodopseudomonas palustris WP_027278865 Rhodovulum sp. PH10 WP_008388541 Bosea sp. Root483D1 WP_057188179 Afifella pfennigii WP_026379319 Parvularcula bermudensis WP_013299881 59 78Sphingopyxis WP_058454986 63 Zymomonas mobilis WP_013933871 Sphingomonas sp. Root710 WP_056378881 100 Blastomonas sp. CACIA14H2 ESZ87453 13 73 Sphingobium baderi LL03 EQB03304 94 95 Sphingobium sp. Leaf26 WP_056683632 100Sphingobium sp. AP49 WP_007710407 100Pantholops hodgsonii XP_005959125 94Sphingobium yanoikuyae WP_004210200 97Sphingobium yanoikuyae WP_017499336 39Shigella sonnei Ss046 YP_309046 Parachlamydia acanthamoebae UV-7 YP_004651412 71 99 Phycisphaera mikurensis NBRC 102666 YP_005445287 Salinibacter ruber M8 YP_003571365 Bacteroides thetaiotaomicron VPI-5482 NP_813526 7299 Stigmatella aurantiaca DW4/3-1 YP_003953245 Sphaerobacter thermophilus DSM 20745 YP_003318973 99 93 Clostridium sp. KLE 1755 WP_021634602 Amycolatopsis mediterranei S699 YP_006555506 68 Thermotoga maritima MSB8 YP_008990654 93 99 Natrialba magadii ATCC 43099 YP_003478720 98 Methanosarcina acetivorans C2A NP_616412 58 Eggerthella sp. YY7918 WP_013981098 98 99 Nostoc sp. PCC 7120 WP_010996602 97 Conexibacter woesei DSM 14684 YP_003392459 Roseiflexus sp. RS-1 YP_001277557 40 Nostoc sp. PCC 7120 WP_010997141 99 Cyanobium sp. PCC 7001 WP_006909232 100 100 100 Symbiodinium sp. Mp CAMPEP_0169859942 Symbiodinium sp. Mp CAMPEP_0169736894 100 97 Togula jolla CCCM 725 CAMPEP_0170596502 78 100 Euglena gracilis Euggra765_c0_seq1 Eutreptiella gymnastica NIES-381 CAMPEP_0113756204 100 84 Chlamydomonas reinhardtii XP_001696432 Chlorella variabilis XP_005848047 98 Micromonas sp. RCC299 XP_002504599 92 Populus trichocarpa XP_006389517 59 81 Coccomyxa subellipsoidea C-169 XP_005648953 99 32 51Galdieria sulphuraria XP_005705015 Ectocarpus siliculosus CAV31258 Chondrus crispus YP_007627389 24 Pavlovales sp. CCMP2436 Pavl23765_c0_seq1 97 Cyanidioschyzon merolae strain 10D NP_849068 Spirochaeta26 smaragdinae DSM 11293 YP_003804890 Candidatus Nitrospira defluvii YP_003797385 98 Bigelowiella natans CCMP2755 Bigna_92341 Porphyridium purpureum Porpur_531.21 95 Aureococcus anophagefferens Auran_23003 100 Gymnodinium catenatum GC744 CAMPEP_0117618956 100 Gymnodinium catenatum GC744 CAMPEP_0117462886 Phaeodactylum tricornutum CCAP 1055/1 XP_002177297 76100 99 Ectocarpus siliculosus CBN79170 100 Plasmopara halstedii CEG49879 Phytophthora sojae XP_009528010 Phytophthora infestans T30-4 XP_002898043 93 Blastocystis sp. NandII AFL03352 47 Blastocystis hominis CBK19749 100 Isochrysis aff. galbana Isogal36468_c0_seq3 95 Pavlovales sp. CCMP2436 Pavl30_c0_seq13 91 Pavlovales sp. CCMP2436 Pavl30_c0_seq10 90 Pavlovales sp. CCMP2436 Pavl30_c0_seq5 100 Kipferlia bialata c5027_g1_i1 90 Arabidopsis lyrata subsp. lyrata XP_002868566 89 99 Coccomyxa subellipsoidea C-169 XP_005651681 Pavlovales sp. CCMP2436100 Pavl30_c0_seq6 92 35Pavlovales sp. CCMP2436 Pavl30_c0_seq7 94 Cyanophora paradoxa Cpara13217 Goniomonas sp m CAMPEP_0114553890 Guillardia theta CCMP2712 XP_005823246 99 Ostreococcus tauri XP_003078939 80 98 Capsicum annuum XP_016539370 99 Gonium pectorale KXZ50550 97 Coccomyxa subellipsoidea C-169 XP_005647349 56 Cryptosporidium hominis TU502 XP_666950 100 100 Babesia equi XP_004829460 Theileria parva strain Muguga XP_765320 100 Neospora caninum Liverpool XP_003886284 100 Toxoplasma gondii ME49 XP_002367417 100 Perkinsus marinus ATCC 50983 XP_002788537 77 Symbiodinium sp. Mp CAMPEP_0169738830 100 Karlodinium micrum CCMP2283 CAMPEP_0169212552 100 Gymnodinium catenatum GC744 CAMPEP_0117561614 100 Paramecium tetraurelia strain d4-2 XP_001455905 100 Ichthyophthirius multifiliis XP_004036646 Tetrahymena thermophila XP_001007588 100 Tetrahymena thermophila XP_001030821 100 Ichthyophthirius multifiliis XP_004034829 7099 100 Neurospora crassa OR74A XP_961753 Capsaspora owczarzaki ATCC 30864 XP_004346702 Gallus gallus NP_001006147 97 Monosiga brevicollis MX1 XP_001750629 67 Salpingoeca rosetta XP_004988486 74 100 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168563690 93 68 Dictyostelium purpureum XP_003283943 Trichosphaerium sp Am-I-7 wt CAMPEP_0168528466 54 Stereomyxa ramosa Chinc5 CAMPEP_0174251850 76 100 Acanthamoeba castellanii str. Neff XP_004339232 MayorellaTric_XP_001582674 sp BSH-02190019 CAMPEP_0174247720 71 Giar_XP_001710092 g10406.t1@[email protected] Page 1 of 1

0.5

99 Dehalococcoides mccartyi 195 YP_180896 98 Neobodo designis CCAP 1951/1 CAMPEP_0174867010 99 98 Mariprofundus ferrooxydans WP_009849432 Caldilinea aerophila DSM 14535 = NBRC 104270 YP_005440529 Aciduliprofundum boonei T469 YP_003483178 98 Selenomonas sputigena ATCC 35185 YP_004413940 99 Thermotoga maritima MSB8 NP_228042 Sphaerobacter thermophilus DSM 20745 YP_003319495 95 97 97 Methylacidiphilum infernorum V4 YP_001939882 Ktedonobacter racemifer WP_007910443 99 Methanobacterium sp. AL-21 YP_004291703 96 55 Anaerolinea thermophila UNI-1 YP_004174406 98 Thermococcus kodakarensis KOD1 YP_184026 57 Sebaldella termitidis ATCC 33386 YP_003310365 76 Dehalococcoides mccartyi 195 YP_181467 73 Synergistes sp. 3_1_syn1 WP_008708833 81 Candidatus Nitrospira defluvii WP_013250064 75 74 Shigella sonnei Ss046 YP_311221 Phycisphaera mikurensis NBRC 102666 YP_005445725 Amycolatopsis mediterranei S699 YP_005528487 74 78 Caldilinea aerophila DSM 14535 = NBRC 104270 YP_005440810 Roseiflexus sp. RS-1 WP_011956963 Candidatus Nitrospira defluvii WP_013247209 Sphingobacterium sp. 21 YP_004316824 99 100 Kipferlia bialata g10406.t1 79 QUERY_g10406.t1_GCS_NuoF 77 Tric_XP_001321469 81 Porphyridium purpureum Porpur_3442.9 Chlorella variabilis XP_005850918 Sawyeria marylandensis ABU95426 64 Dictyostelium purpureum XP_003293586 62 Trichosphaerium sp Am-I-7 wt CAMPEP_0168513096 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168555366 Mayorella sp BSH-02190019 CAMPEP_0174240580 97100 Acanthamoeba castellanii str. Neff XP_004334975 67 59 Stereomyxa ramosa Chinc5 CAMPEP_0174258944 Heterobasidion irregulare TC 32-1 XP_009545866 Homo sapiens NP_009034 4499 Salpingoeca rosetta XP_004992882 98 Monosiga brevicollis MX1 XP_001746110 100100 Euglena gracilis Euggra1856_c0_seq1 84 Eutreptiella gymnastica NIES-381 CAMPEP_0113748202 Neobodo designis CCAP 1951/1 CAMPEP_0174851394 55 Bodo saltans BS70355 100 Trypanosoma cruzi strain CL Brener XP_803973 100 Phytomonas sp. isolate EM1 CCW61292 100 Leishmania infantum JPCM5 XP_001463061 99 Leishmania braziliensis MHOM/BR/75/M2904 XP_001561912 42 Naegleria100 gruberi strain NEG-M XP_002676765 Goniomonas sp m CAMPEP_0114553692 81 53 Guillardia theta CCMP2712 XP_005824796 100 Blastocystis hominis CBK24378 96 100 Gymnodinium catenatum GC744 CAMPEP_0117585254 78 83 Gymnodinium catenatum GC744 CAMPEP_0117478944 Thalassiosira pseudonana CCMP1335 XP_002293580 Aureococcus anophagefferens Auran_24188 24 98 Ectocarpus siliculosus CBJ48598 100 100Phytophthora infestans T30-4 XP_002904800 88100 Phyllostaurus sp. RhEST0000899 88 Astrolonche sp. RhEST0032891 Gromia sphaerica RhEST0023867 72 Reticulomyxa filosa ETO35987 35 68 Nyctotherus ovalis AAU14243 65 Paramecium tetraurelia strain d4-2 XP_001435452 Tetrahymena thermophila SB210 XP_001017144 75 10091 Isochrysis aff. galbana Isogal38910_c0_seq1 Pavlovales sp. CCMP2436 Pavl1185_c0_seq1 Corallomyxa tenera Corten1115_c0_seq1 76 Bigelowiella natans CCMP2755 Bigna_85575 3475 Cyanophora paradoxa Cpara26622 Cyanidioschyzon merolae strain 10D XP_005534915 99 Vigna angularis KOM33919 59 Micromonas commoda XP_002503039 73 Coccomyxa subellipsoidea C-169 XP_005650585 99 Chlorella variabilis XP_005846008 97 Volvox carteri f. nagariensis XP_002948368 100Corallomyxa tenera Corten344_c0_seq1 Parvibaculum lavamentivorans WP_012112155 79 Novosphingobium sp. KN65.2 WP_054948086 Octadecabacter temperatus WP_049834900 98 95 Bosea sp. WAO KUL94801 Hyphomicrobium denitrificans WP_013215897 99 Thermopetrobacter sp. TC1 WP_038034340 100Mesorhizobium WP_023670819 99 Aurantimonas sp. Leaf443 WP_055852063 Phyllobacterium sp. YR531 WP_008125212 99 35 Pseudaminobacter salicylatoxidans WP_019173896 94 Paramesorhizobium deserti KXF77662 Brucella abortus WP_006117021 79 Agrobacterium rhizogenes WP_034507526 70 100Neorhizobium galegae WP_038541986 100Rhizobium sp. Root1220 WP_056537772 65 Rhizobium sullae WP_027508535 Rhizobium mesoamericanum WP_007531789 9410088Rhizobium etli WP_038688201 2195Rhizobium leguminosarum WP_025394097 72 Rhizobium leguminosarum WP_029872847 9310092Rhizobium WP_003547378 4346Rhizobium WP_012483433 Rhizobium WP_007823884 Ensifer WP_025426586 Agrobacterium vitis WP_060715770 76Rhizobium sp. LC145 WP_046792773 Sinorhizobium arboris WP_028000076 70 Bartonella henselae WP_011180783 77 Rhizobium sp. Root149 WP_062552629 75 100Agrobacterium tumefaciens WP_003502381 Agrobacterium WP_006312708 99100100Agrobacterium WP_020810944 94Agrobacterium tumefaciens WP_003521520 689999Pantholops hodgsonii XP_005966783 73 Agrobacterium tumefaciens WP_003496455 8199Rhizobium sp. Leaf262 WP_062451388 Rhizobium undicola WP_027486180 Rhizobium WP_062453233 68Rhizobium sp. NT-26 WP_052637909 78 Acetobacter malorum WP_061492157 SAR116 cluster alpha proteobacterium HIMB100 WP_009603664 100 alpha proteobacterium HIMB114 WP_009360305 Fodinicurvata fenggangensis WP_026987506 86 62 Candidatus Odyssella thessalonicensis WP_010298333 Rickettsia philipii WP_014364403 90 Rickettsiales bacterium Ac37b WP_038603941 75 Candidatus Arcanobacter lacustris KKB96730 84 endosymbiont of Acanthamoeba sp. UWC36 WP_039458234 Anaplasma marginale WP_010267794 Wolbachia endosymbiont of Onchocerca volvulus WP_025264306 100 Wolbachia endosymbiont of Cimex lectularius WP_041045789 100 10099Vollenhovia emeryi XP_011860193 100 Wolbachia endosymbiont of Muscidifurax uniraptor WP_006279611 95 98 Wolbachia pipientis WP_006014035 86Wolbachia endosymbiont strain TRS of Brugia malayi AAW71062 Neorickettsia risticii WP_015816557 60 Priapulus caudatus XP_014680357 83 Burkholderia multivorans ATCC 17616 YP_001579221 100 Legionella pneumophila str. Corby YP_001252304 99 Candidatus Kryptonium thompsoni CUS82342 Chthoniobacter flavus WP_006977854 Mariprofundus ferrooxydans WP_018294247 3756 Stigmatella aurantiaca DW4/3-1 YP_003951276 g10837.t1@[email protected] Page 1 of 1

0.5 100 Thermoplasmatales archaeon BRNA1 WP_015492083 Selenomonas sputigena ATCC 35185 YP_004412886

Vibrio campbellii ATCC BAA-1116 YP_001443609 100Kipferlia bialata g10837.t1 100 QUERY_g10837.t1_ATP_HydG 96 Kipferlia bialata c6565_g1_i1 53 Trepomonas sp. PC1 AGV05426 100 Spironucleus salmonicida AFV80055 Tric_XP_001313153

61 91 Paratrimastix pyriformis AGH33867 100 Sawyeria marylandensis ABU95434 94 Naegleria gruberi strain NEG-M XP_002671139 Acanthamoeba castellanii str. Neff XP_004344725 72 66 Nannochloropsis gaditana CCMP526 XP_005854541 100 Chlamydomonas reinhardtii XP_001691319 67 100 Vitrella brassicaformis CCMP3155 CEM24055 100 99 Tetraselmis sp. GSL018 AHH85812 100 Chlorella variabilis XP_005849755 Porphyridium purpureum Porpur_2123.4

Chlamydiae bacterium SM23_39 KPK32773

82 Acetivibrio cellulolyticus WP_010249740 Candidatus Desulforudis audaxviator MP104C ACA58727

49 Halobacteroidaceae bacterium T328-2 KXS44064 Syntrophaceticus schinkii WP_044664702 97 Desulfovibrio fructosivorans WP_005992322 99 50 Paenibacillus terrae WP_014282030 99 Thermoanaerobacter kivui WP_049684676

40 Cloacibacillus evryensis WP_034444819 Clostridium WP_043032630 50 Lachnospiraceae bacterium mt14 WP_053984810 67 Holophaga foetida WP_043486777

89 Proteocatella sphenisci WP_028829840

48 91 Peptostreptococcaceae bacterium CM5 WP_009529122 Brachyspira hampsonii WP_008724940

100 [Clostridium] methylpentosum WP_006355633 89 Intestinibacter bartlettii DORA_8_9 ETI95763

55 [Clostridium] propionicum DSM 1682 AMJ41046 98 Clostridium homopropionicum WP_052221867 Blautia sp. CAG:257 CDA04972

49 Bacteroides sp. CAG:875 CDD48814 89 Bacteroides intestinalis CAG:315 CDD95821

Bacteroides pyogenes WP_027325067 95 77 Bacteroides sp. 1_1_14 EFI02521 99 95 Bacteroides reticulotermitis WP_044165386 93 Bacteroides faecichinchillae WP_033886979 91 Bacteroides finegoldii CL09T03C10 EKJ92722 96 Bacteroides sp. CAG:754 CDA84822 97 Bacteroides fragilis str. 3397 T10 EXY32106 98 41 99Bacteroides xylanisolvens WP_008025879 99 Bacteroides sp. 3_1_13 WP_049703441 100 82Bacteroides WP_004311777 80 Bacteroides WP_032843884 78 64Bacteroides sp. D2 WP_008999727

Bacteroides sp. D22 WP_009039279

Porphyromonas canoris WP_036792384

Acidaminococcus fermentans WP_012937623 74 Selenomonas sputigena ATCC 35185 YP_004412879 g10857.t1@[email protected] Page 1 of 1

0.2

Thermotoga maritima MSB8 YP_008991215 99 100 Entamoeba invadens IP1 XP_004257277 Entamoeba dispar SAW760 XP_001740105 Entamoeba histolytica HM-1:IMSS XP_655257 94 Spirochaeta100 smaragdinae DSM 11293 YP_003804862 82 Methanobacterium sp. AL-21 YP_004291042 Stereomyxa ramosa Chinc5 CAMPEP_0174277732 Trichosphaerium sp Am-I-7 wt CAMPEP_0168538322 100 Cenarchaeum symbiosum A YP_876496 66 82 Hydrogenobacter thermophilus TK-6 YP_005511830 53 Nostoc sp. PCC 7120 NP_487128 Synergistes sp. 3_1_syn1 WP_008710780 64 90 marine actinobacterium PHSC20C1 WP_009772456 100 Thermoplasmatales archaeon BRNA1 YP_007688599 54 Clostridium sp. KLE 1755 WP_021636656 68 Methanosarcina acetivorans C2A NP_617617 100 Methanobacterium sp. AL-21 YP_004290429 100 Tritrichomonas foetus AAQ01175 78 Tric_XP_001320829 77 Perkinsus marinus ATCC 50983 XP_002768987 Neospora caninum Liverpool XP_003880745 60 Toxoplasma gondii ME49 XP_002371254 100 99 Plasmodium chabaudi chabaudi XP_745824 69 Babesia equi XP_004830262 100 Theileria parva strain Muguga XP_765551 98100 CryptosporidiumQUERY_g10857.t1_ISC_IscS hominis TU502 XP_668649 Kipferlia bialata c6588_g1_i1 70 100 Symbiodinium sp. CCMP2430 CAMPEP_0181639934 75 Symbiodinium sp. CCMP2430 CAMPEP_0181700790 Prorocentrum minium CCMP2233 CAMPEP_0176923436 99 100 Euplotes vannus CAH04407 Oxytricha trifallax EJY69193 100 Ichthyophthirius multifiliis XP_004037079 67 95 Tetrahymena thermophila XP_001007310 95 Paramecium tetraurelia strain d4-2 XP_001428522 Tetrahymena thermophila SB210 XP_001012657 97 Ichthyophthirius multifiliis XP_004037398 100 Eutreptiella gymnastica NIES-381 CAMPEP_0113740608 Perkinsela sp. CCAP 1560/4 KNH05525 Bodo saltans BS16795 99 94 99 Phytomonas sp. isolate EM1 CCW60975 Leishmania braziliensis MHOM/BR/75/M2904 XP_001565874 100 Leishmania infantum JPCM5 XP_001466341 100 Trypanosoma cruzi strain CL Brener XP_816580 100 98 Trypanosoma brucei brucei TREU927 XP_828256 100 Neobodo designis CCAP 1951/1 CAMPEP_0174852178 Cyanophora paradoxa Cpara05305 59 54 Eucalyptus grandis KCW54704 Chlorella variabilis XP_005843562 57 Plasmodiophora brassicae CEP03584 92 Ostreococcus tauri XP_003078141 Chlamydomonas reinhardtii XP_001695008 55 Micromonas commoda XP_002501646 51 Penicillium oxalicum 114-2 EPS27133 94 62 Trichinella murrelli KRX43588 73 Fonticula alba XP_009497079 50 Hydra vulgaris XP_002167506 52 Caenorhabditis remanei XP_003114825 Monosiga brevicollis MX1 XP_001749755 60 52 Salpingoeca rosetta XP_004994401 100 Capsaspora owczarzaki ATCC 30864 KJE95565 53 95 Exaiptasia pallida KXJ12788 Trichoplax adhaerens XP_002108276 100 Trachymyrmex cornetzi KYN12916 99 Ceratitis capitata XP_004537786 Lingula anatina XP_013404050 64 100 Pundamilia nyererei XP_005729699 81 Latimeria chalumnae XP_005986781 87 Caprimulgus carolinensis KFZ53991 45 91 100 Trichechus manatus latirostris XP_004370451 Loxodonta africana XP_003411731 87 100 Rhinopithecus roxellana XP_010384859 98 Monodelphis domestica XP_001381508 50 Microtus ochrogaster XP_005363114 87 49Ceratotherium simum simum XP_004430517 99 77Pantholops hodgsonii XP_005968098 80Myotis davidii XP_006764615 42 Xenopus tropicalis XP_002932937 Stegodyphus mimosarum KFM65597 75 Branchiostoma floridae XP_002612481 86 Pavlovales sp. CCMP2436 Pavl662_c0_seq1 Chrysochromulina sp. CCMP291 KOO23614 53 100 Isochrysis aff. galbana Isogal7964_c0_seq1 99 Emiliania huxleyi CCMP1516 XP_005792565 97100 Albugo laibachii Nc14 CCA24341 100 Plasmopara halstedii CEG47472 Phytophthora parasitica P1569 ETI39442 100 Saprolegnia parasitica CBS 223.65 XP_012206110 100 Aphanomyces invadans XP_008861719 74100 Nannochloropsis gaditana EWM27642 Thalassiosira pseudonana CCMP1335 XP_002287162 100 Gymnodinium catenatum GC744 CAMPEP_0117470760 46 Gymnodinium catenatum GC744 CAMPEP_0117584922 96 Ectocarpus siliculosus CBN75233 79 100 Aureococcus anophagefferens XP_009032790 Aureococcus anophagefferens Auran_18852 100 Chondrus crispus XP_005717438 100 Goniomonas sp m CAMPEP_0114556894 Guillardia theta CCMP2712 XP_005827479 49 Mayorella sp BSH-02190019 CAMPEP_0174245736 84 66 Dictyostelium lacteum KYR00479 78 Dictyostelium fasciculatum XP_004366652 100 Acytostelium subglobosum LB1 XP_012750597 4179 100 Polysphondylium pallidum PN500 EFA85765 100Dictyostelium purpureum XP_003283880 Acanthamoeba castellanii str. Neff XP_004344047 99 Stereomyxa ramosa Chinc5 CAMPEP_0174262254 100 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168549242 40 Bigelowiella natans CCMP2755 Bigna_55226 89 Reticulomyxa filosa ETO13652 86 Trichosphaerium sp Am-I-7 wt CAMPEP_0168539756 Papilio xuthus XP_013170352 79 Sawyeria marylandensis ABU95428 31 Spironucleus salmonicida AFV80043 49 Giardia intestinalis ESU38083 100 Blastocystis sp. NandII AFL03350 45 80 Blastocystis hominis XP_012898233 100 Blastocystis hominis CBK24185 Thalassospira100 WP_062952118 98 Rickettsia massiliae WP_012152913 96 Anaplasma WP_012658969 71 Trichoplax adhaerens XP_002118977 79 Vibrio campbellii ATCC BAA-1116 YP_008526293 76 98 Burkholderia multivorans ATCC 17616 YP_001946553 Nitrospira defluvii WP_013250513 77 Synechococcus sp. CB0101 WP_010305183 Burkholderia multivorans ATCC 17616 YP_001585152 82 Ktedonobacter racemifer WP_007916548 Sphingobacterium sp. 21 WP_013666285 85 Cenarchaeum symbiosum A YP_875595 100 Nematostella vectensis XP_001622745 100 Bigelowiella natans CCMP2755 Bigna_92143 Stereomyxa ramosa Chinc5 CAMPEP_0174268508 Sphaerobacter thermophilus DSM 20745 YP_003320879 83 100 Denitrovibrio acetiphilus DSM 12809 YP_003505118 g11348.t1@[email protected] Page 1 of 1

0.5

65 Clostridium sp. KLE 1755 WP_021634687 67 Synergistes sp. 3_1_syn1 WP_008712669 SAR202 cluster bacterium SCGC AAA240-N13 WP_017953409 63 Aciduliprofundum boonei T469 YP_003483072 Legionella pneumophila str. Corby YP_001249481 80 Candidatus Caldiarchaeum subterraneum YP_008797907 79 100 Conexibacter woesei DSM 14684 YP_003395546 49 95 Kipferlia bialata g1309.t1 Kipferlia bialata c7809_g1_i1 Mariprofundus ferrooxydans WP_018294187 54 Thermotoga maritima MSB8 NP_228028 9948Kipferlia bialata g11348.t1 100 QUERY_g11348.t1_GCS_GCSP2 Kipferlia bialata c7761_g1_i1 100 Kipferlia bialata g5795.t1 99 Rhodomicrobium udaipurense WP_037239391 99 Paratrimastix pyriformis ABW76104 87 97 Mariprofundus ferrooxydans WP_018294186 Pyrinomonas methylaliphatogenes WP_041975897 93 Anaerolinea thermophila UNI-1 YP_004174306 83 Acidimicrobium ferrooxidans DSM 10331 YP_003109724 Natrialba magadii ATCC 43099 YP_003478546 69 Ktedonobacter racemifer WP_007912619 86 Rhodopirellula baltica SH 1 NP_867901 75 Thermotoga maritima MSB8 YP_008990812 99 Clostridium cylindrosporum WP_048571325 Streptococcus pneumoniae WP_061815585 Exiguobacterium mexicanum WP_034778005 100 95 Listeria monocytogenes WP_014930721 99 Fictibacillus sp. FJAT-27399 WP_062235042 99 Halobacillus sp. BBL2006 WP_035547954 Bacillus subtilis group WP_035338368 54 97 Bacillus sp. FJAT-25509 WP_056475082 99 Bacillus horikoshii WP_063560308 95 Bacillus manliponensis WP_034638906 100 94 Bacillus sp. 123MFChir2 WP_020061614 100Bacillus cereus WP_016132011 Bacillus gaemokensis WP_033672507 95Bacillus sp. LK2 WP_048374172 86 10099Bacillus cereus WP_002111798 99Bacillus cereus group WP_048563832 63 Bacillus cereus WP_061677399 9499Bacillus cereus WP_016118623 Bacillus cereus WP_011110376 99Bacillus bombysepticus WP_044584857 19Bacillus cereus WP_000795704 9798Bacillus cereus WP_063260822 96 96Bacillus cereus Rock3-42 EEL43907 3Bacillus WP_000795696 6Streptococcus pneumoniae CJB73425 Bacillus thuringiensis WP_030028049 5Bacillus mycoides WP_042979862 96 Synergistes sp. 3_1_syn1 WP_008712667 Candidatus Caldiarchaeum subterraneum WP_020864503 96 Thermococcus kodakarensis KOD1 YP_183792 Parachlamydia acanthamoebae UV-7 YP_004651046 Legionella pneumophila str. Corby YP_001249479 Vitellibacter sp. D-24 WP_062621735 100 Acanthamoeba castellanii str. Neff XP_004341789 39 Stereomyxa ramosa Chinc5 CAMPEP_0174262296 Reticulomyxa filosa ETO18751 28 Guillardia theta CCMP2712 XP_005825076 92 Cyanophora paradoxa Cpara17629 100Fischerella sp. PCC 9339 WP_017309685 29 100 Acytostelium subglobosum LB1 XP_012758533 3299 Polysphondylium pallidum PN500 EFA86802 Dictyostelium purpureum XP_003287462 48 Mayorella sp BSH-02190019 CAMPEP_0174247374 100 Eutreptiella gymnastica NIES-381 CAMPEP_0113747296 90 Euglena gracilis Euggra1419_c0_seq1 90 Bigelowiella natans CCMP2755 Bigna_49958 79 Reticulomyxa filosa ETO18752 Plasmodiophora brassicae CEO97849 Trichosphaerium sp Am-I-7 wt CAMPEP_0168509054 77 7194 Goniomonas sp m CAMPEP_0114554758 55 Chondrus crispus XP_005711074 Chondrus crispus XP_005716298 96100 Perkinsus marinus ATCC 50983 XP_002785345 Heterocapsa rotundata SCCAP K-0483 CAMPEP_0168659994 84 Prorocentrum minium CCMP2233 CAMPEP_0176686804 76 100 Prorocentrum minium CCMP2233 CAMPEP_0176694350 100Blastocystis hominis CBK22590 100 Gymnodinium catenatum GC744 CAMPEP_0117601206 69 Gymnodinium catenatum GC744 CAMPEP_0117595782 73 Gymnodinium catenatum GC744 CAMPEP_0117460470 56 Phytophthora infestans T30-4 XP_002898087 33 Aureococcus anophagefferens Auran_59319 97 70 Ectocarpus siliculosus CBJ30901 94 Phaeodactylum tricornutum CCAP 1055/1 XP_002182636 62 71 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168573994 Paracoccidioides brasiliensis Pb03 EEH21406 Saccharomyces cerevisiae AWRI1631 EDZ70044 87 Capsaspora owczarzaki ATCC 30864 XP_004343370 88 Neolamprologus brichardi XP_006790096 76 Salpingoeca rosetta XP_004991539 67 Monosiga brevicollis MX1 XP_001749579 54100 100Isochrysis aff. galbana Isogal36766_c0_seq1 Pavlovales sp. CCMP2436 Pavl248_c1_seq2 91 Ostreococcus lucimarinus CCE9901 XP_001420206 Chlorella variabilis XP_005842622 73 98 Chlorella variabilis XP_005850088 93 Coccomyxa subellipsoidea C-169 XP_005649750 100 95 Volvox carteri f. nagariensis XP_002956892 Populus trichocarpa XP_002338013 Glycine max XP_003523643 75 100 Paramecium tetraurelia strain d4-2 XP_001441797 Cyanophora paradoxa Cpara01861 Ichthyophthirius multifiliis XP_004024418 76 Tetrahymena thermophila XP_001020921 66 97 Neobodo designis CCAP 1951/1 CAMPEP_0174827230 Bodo saltans BS03790 96 98 Leishmania braziliensis MHOM/BR/75/M2904 XP_001562237 92 Leishmania infantum JPCM5 XP_001470353 96 Trypanosoma cruzi strain CL Brener XP_812963 100 Trypanosoma brucei brucei strain 927/4 GUTat10.1 XP_845815 g11532.t1@[email protected] Page 1 of 1

0.2

Aquifex aeolicus WP_010881213

Candidatus Phaeomarinobacter ectocarpi WP_043950754

53

Rhodovibrio salinarum WP_027289044

92

Azospirillum sp. B506 WP_042703379

42

Capsicum annuum XP_016559866

59

69 Eufriesea mexicana OAD55618

59

Chondrus crispus XP_005716304

Bosea sp. WAO KUL96244

59

Giardia lamblia ATCC 50803 XP_001707906

Tric_XP_001316269 g11968.t1@[email protected] Page 1 of 1

0.2 Branchiostoma floridae XP_002594084 70 Thermotoga maritima 1ZKO_A 76 Plasmodium chabaudi chabaudi XP_739104 69 Dictyostelium purpureum XP_003290169 76 Waddlia chondrophila WP_041941456 72 Exophiala dermatitidis NIH/UT8656 XP_009153752 92 Monosiga brevicollis MX1 XP_001748868 100 Eutreptiella gymnastica NIES-381 CAMPEP_0113761184 Euglena gracilis Euggra422_c0_seq1 100 Eutreptiella gymnastica NIES-381 CAMPEP_0113760080 77 Tric_XP_001299513 91 Goniomonas sp m CAMPEP_0114539136 Bigelowiella natans CCMP2755 Bigna_91378 86 Acanthamoeba castellanii str. Neff XP_004356599 Guillardia theta CCMP2712 XP_005828260 56 Selaginella moellendorffii XP_002980848 99 Cyanidioschyzon merolae strain 10D XP_005535759 59 98 Roseiflexus sp. RS-1 YP_001275868 91 Sphingobacterium sp. 21 YP_004317422 Thermogemmatispora carboxidivorans WP_052887433 94 Anaerolinea thermophila UNI-1 YP_004174304 Mariprofundus ferrooxydans WP_009850108 92 Syntrophobacter sp. DG_60 KPJ63842 61 Halothermothrix orenii WP_015924056 48 74 Thermomicrobium roseum DSM 5159 ACM05575 70 55 Lingula anatina XP_013381406 54 Thermococcus kodakarensis KOD1 YP_182563 84 84 Candidatus Caldiarchaeum subterraneum YP_008797326 40 56 97 Hydrogenobacter thermophilus TK-6 YP_003433185 77 Leptolyngbya sp. O-77 BAU41308 Herpetosiphon geysericola WP_054534200 72 Tepidanaerobacter acetatoxydans WP_013777472 67 Aciduliprofundum boonei T469 YP_003483698 66 Aneurinibacillus migulanus WP_043064158 65 Deltaproteobacteria bacterium GWC2_55_46 KYK98584 68 Pyrinomonas methylaliphatogenes WP_041975900 88 Stigmatella aurantiaca DW4/3-1 YP_003953230 Medicago truncatula XP_003611464 40 Physcomitrella patens XP_001769387 92 Prevotella WP_004338651 35 Micromonas pusilla CCMP1545 XP_003064360 84 100 Coccomyxa subellipsoidea C-169 XP_005646133 100 Chlamydomonas reinhardtii XP_001696637 100 Isochrysis aff. galbana Isogal17383_c0_seq1 Emiliania huxleyi CCMP1516 XP_005789351 74 Rhizopus delemar RA 99-880 EIE81545 Cardiobacterium valvarum WP_040389348 72 Absidia glauca SAM04653 76 96 Gonapodya prolifera JEL478 KXS21353 Batrachochytrium dendrobatidis JAM81 XP_006676156 72 87 Sphingobium sp. Leaf26 WP_056683090 Pavlovales sp. CCMP2436 Pavl239_c0_seq1 Hyphomonas atlantica WP_035553057 91 47 Labrenzia WP_029062683 65 Thermopetrobacter sp. TC1 WP_038035305 74 Candidatus Pelagibacter sp. IMCC9063 WP_013695627 99 100 Candidatus Pelagibacter ubique WP_023648118 65 100 uncultured marine microorganism HF4000_093M11 unclassified ABZ06555 Brevundimonas nasdae WP_039245431 Caulobacter sp. Root655 WP_056721060 95 Caulobacter sp. CCH5-E12 WP_062097190 100 Caulobacter segnis WP_013077528 Pantholops hodgsonii XP_005979686 100 96 99 96 Phenylobacterium WP_056016609 97 94 Caulobacter vibrioides WP_004615722 Caulobacter WP_047411812 Caulobacter sp. Root656 WP_057185636 99 Caulobacter vibrioides WP_058348589 100Ricinus communis XP_002538371 100 Kipferlia bialata g11968.t1 56 QUERY_g11968.t1_GCS_GCSH 47 Ectocarpus siliculosus CBJ48668 98 Albugo candida CCI41073 48 Phytophthora infestans T30-4 XP_002901673 64 Saprolegnia diclina VS20 XP_008604936 72 71 Blastocystis hominis CBK21270 Thalassiosira pseudonana CCMP1335 XP_002291369 Nannochloropsis gaditana CCMP526 XP_005854003 64 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168578600 100 Karlodinium micrum CCMP2283 CAMPEP_0169261390 100 68 Karlodinium micrum CCMP2283 CAMPEP_0169379678 Peridinium aciculiferum PAER-2 CAMPEP_0183548144 71 Mus musculus XP_006543099 g12202.t1@[email protected] Page 1 of 1

0.1 Tritrichomonas foetus AAK55144

Tric_XP_001324407

Sphingomonas sp. Leaf230 WP_056063439

80

Spironucleus salmonicida AFV80041

100

Giardia intestinalis ABV81962

57

Giardia intestinalis ABV81967

32

Giardia intestinalis ABV81980

61 31

Giardia intestinalis ABV81969

32 Giar_ABV81956

83 Giardia intestinalis ABV81982

95 Giardia intestinalis ABV81979

Giardia intestinalis ACJ35896 g12941.t1@[email protected] Page 1 of 1

0.2

Desulfovibrio alkalitolerans WP_020886242

Paratrimastix pyriformis ABW76103 81

Kipferlia bialata c7809_g1_i1

Micavibrio aeruginosavorus WP_015467686

Tistrella mobilis WP_062763540 39 75

Devosia riboflavina WP_035087606

66 Candidatus Odyssella thessalonicensis WP_029448069

Rhodomicrobium vannielii WP_013417728 53

Azospirillum sp. B4 WP_044564630 41

alpha proteobacterium JLT2015 WP_008600084 30

Erythrobacter sp. HI0074 WP_063506348 100

Sphingopyxis macrogoltabida WP_054588442 100

Sphingomonas sp. Ant20 WP_037530514 92

Sphingomonas sanxanigenens WP_025291584

73 Sphingobium japonicum BiD32 CCW16098

94 Sphingobium czechense LL01 KMS55658

Sphingobium sp. DC-2 WP_030539642 69 71

100 Sphingobium japonicum WP_013040939

53 Sphingobium sp. Leaf26 WP_056683087

95 Sphingobium yanoikuyae WP_010336293

65 97 Sphingobium yanoikuyae WP_004207945

92 Pantholops hodgsonii XP_005982532

Sphingomonas sp. SKA58 WP_037543449

77 Sphingobium sp. C100 WP_024018057 g13233.t1@[email protected] Page 1 of 1

0.2 100 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168557850 100 Acanthamoeba castellanii str. Neff XP_004349634 100 Acytostelium subglobosum LB1 XP_012755963 Dictyostelium purpureum XP_003285468 Dictyostelium discoideum AX4 XP_635129 100 100 Isochrysis aff. galbana Isogal5444_c0_seq1 92 Emiliania huxleyi CCMP1516 XP_005789291 94 Pavlovales sp. CCMP2436 Pavl382_c0_seq1 Symbiodinium sp. CCMP2430 CAMPEP_0181657878 Heterocapsa rotundata SCCAP K-0483 CAMPEP_0168633780 100 Togula jolla CCCM 725 CAMPEP_0170637364 90 Bigelowiella natans CCMP2755 Bigna_54879 88 100 Theileria parva strain Muguga XP_765917 77 70 Babesia equi XP_004829871 Oxytricha trifallax EJY70902 Corallomyxa tenera Corten1527_c0_seq2 88 Neospora caninum Liverpool XP_003884541 55 Toxoplasma gondii ME49 XP_002368859 100 Blastocystis hominis CBK21642 83 100 Neobodo designis CCAP 1951/1 CAMPEP_0174850566 100 Bodo saltans BS37060 66 Leishmania braziliensis MHOM/BR/75/M2904 XP_001563435 Leishmania infantum JPCM5 XP_001464319 97 100 Eutreptiella gymnastica NIES-381 CAMPEP_0113767486 Neobodo designis CCAP 1951/1 CAMPEP_0174829316 97 Bodo saltans BS04645 100 Leptomonas seymouri KPI85102 43 81 98 100 Crithidia acanthocephali AGT02678 Leishmania braziliensis MHOM/BR/75/M2904 XP_001566284 100 Leishmania infantum JPCM5 XP_001470279 100 100 Phytomonas sp. isolate EM1 CCW62261 Eutreptiella gymnastica NIES-381 CAMPEP_0113748852 97 Mayorella sp BSH-02190019 CAMPEP_0174232492 85 Monosiga brevicollis MX1 XP_001749016 Salpingoeca rosetta XP_004993173 10089 Aureococcus anophagefferens Auran_53011 89 Gymnodinium catenatum GC744 CAMPEP_0117533970 Phaeodactylum tricornutum CCAP 1055/1 XP_002186147 98 Phytophthora infestans T30-4 XP_002898577 98 Ectocarpus siliculosus CBN75596 70 95 Goniomonas sp m CAMPEP_0114569526 60 Cryptococcus gattii WM276 XP_003193995 99 Vitis vinifera XP_002266312 Micromonas pusilla CCMP1545 XP_003062423 25 Chlamydomonas reinhardtii XP_001701593 100 Chlorella variabilis XP_005852257 21 100 Cyanophora paradoxa Cpara16757 Guillardia theta CCMP2712 XP_005829852 17 Galdieria sulphuraria XP_005706998 59 Cyanidioschyzon merolae strain 10D XP_005537435 100 Emiliania huxleyi CCMP1516 XP_005773799 Isochrysis aff. galbana Isogal33977_c0_seq1 77 Thalassiosira pseudonana CCMP1335 XP_002289669 93 Dinophysis acuminata DAEP01 CAMPEP_0179264138 96 Dinophysis acuminata DAEP01 CAMPEP_0179331896 96 100 100 QUERY_g13233.t1_GCS_SHMT Kipferlia bialata c9069_g1_i1 Tric_XP_001322593 Larimichthys crocea KKF24334 100 Saccoglossus kowalevskii NP_001171839 100 99 Trichinella sp. T8 KRZ95850 Schistosoma japonicum CAX73624 68 91 Stegodyphus mimosarum KFM56921 100 Zea mays ACG44669 79 93 100 Dendroctonus ponderosae ENN76463 99 Polistes dominula XP_015176683 100 Bactrocera cucurbitae XP_011179918 Anopheles sinensis KFB35983 99 89 Zootermopsis nevadensis KDR24282 100 Daphnia pulex Dappu_301155 Nematostella vectensis XP_001625575 99 Saccoglossus kowalevskii XP_002740742 77 Daphnia magna KZS18847 Capitella teleta ELU01860 Ciona intestinalis XP_002126094 99 100 Callorhinchus milii XP_007907505 Latimeria chalumnae XP_005986324 84 Xenopus tropicalis XP_012809510 Cyprinodon variegatus XP_015258360 89 84 Cuculus canorus XP_009564565 69 Sarcophilus harrisii XP_012406985 Propithecus coquereli XP_012507429 37 93 93Ceratotherium simum simum XP_004429349 98 100 Ochotona princeps XP_004582984 Camelus bactrianus XP_010944588 10093Tupaia chinensis XP_006171967 96Chlorocebus sabaeus XP_008001934 93Gorilla gorilla gorilla XP_004053470 Pan paniscus XP_003824922 100Homo sapiens NP_001159828 92 95125Homo sapiens 4PVF_A 56Homo sapiens BAG58915 Homo sapiens NP_001159830 8034Homo sapiens CAI46021 41Homo sapiens BAD97275 synthetic construct other AAP36780 7Homo sapiens EAW96996 Homo sapiens 3OU5_A Homo sapiens NP_005403 Homo sapiens NP_001159831 Homo sapiens NP_001159829 Branchiostoma floridae XP_002609556 Paratrimastix pyriformis AGH33868 66 Trichosphaerium sp Am-I-7 wt CAMPEP_0168530946 Acanthamoeba castellanii str. Neff XP_004352083 93 Stereomyxa ramosa Chinc5 CAMPEP_0174262006 99 Filamoeba nolandi NC-AS-23-1 CAMPEP_0168551794 85 Mastigamoeba balamuthi AIW54426 94 Mayorella sp BSH-02190019 CAMPEP_0174229920 9296100 Helicosporidium sp. ATCC 50920 KDD73295 100 Volvox carteri f. nagariensis XP_002951075 Glycine max NP_001238335 Ostreococcus tauri XP_003084275 94 Guillardia theta CCMP2712 XP_005826504 Goniomonas sp m CAMPEP_0114550914 85 Chondrus crispus XP_005717158 100 Coccomyxa subellipsoidea C-169 XP_005643716 Chlamydomonas reinhardtii XP_001701451 94 Glycine max XP_006601749 68 Ostreococcus tauri XP_003083071 93 99 99 100 Isochrysis aff. galbana Isogal39372_c0_seq1 Emiliania huxleyi CCMP1516 XP_005785878 87 Pavlovales sp. CCMP2436 Pavl333_c1_seq1 Reticulomyxa filosa ETO24232 77 Naegleria gruberi strain NEG-M XP_002683182 97 Bigelowiella natans CCMP2755 Bigna_49865 90 93 Cladophialophora immunda XP_016248100 100 Ichthyophthirius multifiliis XP_004037375 Tetrahymena thermophila XP_001018842 75 67 Paramecium tetraurelia strain d4-2 XP_001428490 97 100 Togula jolla CCCM 725 CAMPEP_0170598852 Scrippsiella trochoidea CCMP3099 CAMPEP_0115222228 Scrippsiella trochoidea CCMP3099 CAMPEP_0115642174 100 Perkinsus marinus ATCC 50983 XP_002781680 100 Perkinsus marinus ATCC 50983 XP_002768194 71 100 Phytophthora parasitica P10297 ETP40070 91 Aureococcus anophagefferens Auran_20758 Nannochloropsis gaditana CCMP526 XP_005852729 90 Ectocarpus siliculosus CBN75444 90 99 Phaeodactylum tricornutum CCAP 1055/1 XP_002177340