Phylogenetic Analysis of Nematodes of the Genus Pratylenchus Using Nuclear 26S Rdna
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4Th National IPM Symposium
contents Foreword . 2 Program Schedule . 4 National Roadmap for Integrated Pest Management (IPM) . 9 Whole Systems Thinking Applied to IPM . 12 Fourth National IPM Symposium . 14 Poster Abstracts . 30 Poster Author Index . 92 1 foreword Welcome to the Fourth National Integrated Pest Management The Second National IPM Symposium followed the theme “IPM Symposium, “Building Alliances for the Future of IPM.” As IPM Programs for the 21st Century: Food Safety and Environmental adoption continues to increase, challenges facing the IPM systems’ Stewardship.” The meeting explored the future of IPM and its role approach to pest management also expand. The IPM community in reducing environmental problems; ensuring a safe, healthy, has responded to new challenges by developing appropriate plentiful food supply; and promoting a sustainable agriculture. The technologies to meet the changing needs of IPM stakeholders. meeting was organized with poster sessions and workshops covering 22 topic areas that provided numerous opportunities for Organization of the Fourth National Integrated Pest Management participants to share ideas across disciplines, agencies, and Symposium was initiated at the annual meeting of the National affiliations. More than 600 people attended the Second National IPM Committee, ESCOP/ECOP Pest Management Strategies IPM Symposium. Based on written and oral comments, the Subcommittee held in Washington, DC, in September 2001. With symposium was a very useful, stimulating, and exciting experi- the 2000 goal for IPM adoption having passed, it was agreed that ence. it was again time for the IPM community, in its broadest sense, to come together to review IPM achievements and to discuss visions The Third National IPM Symposium shared two themes, “Putting for how IPM could meet research, extension, and stakeholder Customers First” and “Assessing IPM Program Impacts.” These needs. -
Description of Pratylenchus Dunensis Sp. N. (Nematoda: Pratylenchidae
Nematology, 2006, Vol. 8(1), 79-88 Description of Pratylenchus dunensis sp.n.(Nematoda: Pratylenchidae), a root-lesion nematode associated with the dune grass Ammophila arenaria (L.) Link ∗ Eduardo DE LA PEÑA 1, , Maurice MOENS 1,2, Adriaan VA N AELST 3 and Gerrit KARSSEN 4,5 1 Agricultural Research Centre, Crop Protection Department, Burg. van Gansberghelaan 96, 9820, Merelbeke, Belgium 2 Gent University, Laboratory for Agrozoology, Coupure 653, 9000 Gent, Belgium 3 Wageningen University & Research Centre, Laboratory of Plant Cell Biology, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands 4 Plant Protection Service, Nematology Section, P.O. Box 9102, 6700 HC Wageningen, The Netherlands 5 Wageningen University & Research Centre, Laboratory of Nematology, Binnenhaven 5, 6709 PD Wageningen, The Netherlands Received: 4 April 2005; revised: 7 November 2005 Accepted for publication: 7 November 2005 Summary – A root-lesion nematode, Pratylenchus dunensis sp. n., is described and illustrated from Ammophila arenaria (L.) Link, a grass occurring abundantly in coastal dunes of Atlantic Europe. The new species is characterised by medium sized (454-579 µm) slender, vermiform, females and males having two lip annuli (sometimes three to four; incomplete incisures only visible with scanning electron microscopy), medium to robust stylet (ca 16 µm) with robust stylet knobs slightly set off, long pharyngeal glands (ca 42 µm), lateral field with four parallel, non-equidistant, lines, the middle ridge being narrower than the outer ones, lateral field with partial areolation and lines converging posterior to the phasmid which is located between the two inner lines of the lateral field in the posterior half of the tail, round spermatheca filled with round sperm, vulva at 78% of total body length and with protruding vulval lips, posterior uterine sac relatively short (ca 19 µm), cylindrical tail (ca 33 µm) narrowing in the posterior third with smooth tail tip and with conspicuous hyaline part (ca 2 µm). -
Use of the Arbuscular Mycorrhizal Fungus Glomus Intraradices As Biological Control Agent of the Nematode Nacobbus Aberrans Parasitizing Tomato
668 Vol.57, n.5: pp. 668-674, September-October 2014 BRAZILIAN ARCHIVES OF http://dx.doi.org/10.1590/S1516-8913201402200 ISSN 1516-8913 Printed in Brazil BIOLOGY AND TECHNOLOGY AN INTERNATIONAL JOURNAL Use of the Arbuscular Mycorrhizal Fungus Glomus intraradices as Biological Control Agent of the Nematode Nacobbus aberrans Parasitizing Tomato Nicolás Marro 1*, Paola Lax 2, Marta Cabello 3, Marcelo Edmundo Doucet 2 and Alejandra Gabriela Becerra 1 1Laboratorio de Micología; Instituto Multidisciplinario de Biología Vegetal; CONICET- Universidad Nacional de Córdoba; Córdoba - Argentina. 2Instituto de Diversidad y Ecología Animal (CONICET-UNC) y Centro de Zoología Aplicada, Facultad de Ciencias Exactas, Físicas y Naturales, Universidad Nacional de Córdoba, Argentina. 3Instituto Spegazzini, Facultad de Ciencias Naturales y Museo, Buenos Aires, Argentina ABSTRACT The plant-parasitic nematode Nacobbus aberrans is an endoparasite that induces gall formation in the roots and causes severe losses to diverse crops. Some populations of this nematode show preference for certain hosts, revealing the existence of “races/groups” with different behaviour and making nematode management difficult. A possible biological control alternative to reduce the damage caused by this species may be the use of arbuscular mycorrhizal fungi (AMF). In the present work, the effect of Glomus intraradices on tomato plants inoculated with the nematode at transplanting and three weeks later was tested. At 60 days, the following parameters were estimated: percentage of AMF colonization, root and aerial dry weight, number of galls and egg masses, and reproduction factor (RF=final population/initial population) of N. aberrans . AMF colonization was higher in the presence of the nematode. -
The Complete Mitochondrial Genome of the Columbia Lance Nematode
Ma et al. Parasites Vectors (2020) 13:321 https://doi.org/10.1186/s13071-020-04187-y Parasites & Vectors RESEARCH Open Access The complete mitochondrial genome of the Columbia lance nematode, Hoplolaimus columbus, a major agricultural pathogen in North America Xinyuan Ma1, Paula Agudelo1, Vincent P. Richards2 and J. Antonio Baeza2,3,4* Abstract Background: The plant-parasitic nematode Hoplolaimus columbus is a pathogen that uses a wide range of hosts and causes substantial yield loss in agricultural felds in North America. This study describes, for the frst time, the complete mitochondrial genome of H. columbus from South Carolina, USA. Methods: The mitogenome of H. columbus was assembled from Illumina 300 bp pair-end reads. It was annotated and compared to other published mitogenomes of plant-parasitic nematodes in the superfamily Tylenchoidea. The phylogenetic relationships between H. columbus and other 6 genera of plant-parasitic nematodes were examined using protein-coding genes (PCGs). Results: The mitogenome of H. columbus is a circular AT-rich DNA molecule 25,228 bp in length. The annotation result comprises 12 PCGs, 2 ribosomal RNA genes, and 19 transfer RNA genes. No atp8 gene was found in the mitog- enome of H. columbus but long non-coding regions were observed in agreement to that reported for other plant- parasitic nematodes. The mitogenomic phylogeny of plant-parasitic nematodes in the superfamily Tylenchoidea agreed with previous molecular phylogenies. Mitochondrial gene synteny in H. columbus was unique but similar to that reported for other closely related species. Conclusions: The mitogenome of H. columbus is unique within the superfamily Tylenchoidea but exhibits similarities in both gene content and synteny to other closely related nematodes. -
Nematodes and Agriculture in Continental Argentina
Fundam. appl. NemalOl., 1997.20 (6), 521-539 Forum article NEMATODES AND AGRICULTURE IN CONTINENTAL ARGENTINA. AN OVERVIEW Marcelo E. DOUCET and Marîa M.A. DE DOUCET Laboratorio de Nematologia, Centra de Zoologia Aplicada, Fant/tad de Cien.cias Exactas, Fisicas y Naturales, Universidad Nacional de Cordoba, Casilla df Correo 122, 5000 C6rdoba, Argentina. Acceplecl for publication 5 November 1996. Summary - In Argentina, soil nematodes constitute a diverse group of invertebrates. This widely distributed group incJudes more than twO hundred currently valid species, among which the plant-parasitic and entomopathogenic nematodes are the most remarkable. The former includes species that cause damages to certain crops (mainly MeloicU:igyne spp, Nacobbus aberrans, Ditylenchus dipsaci, Tylenchulus semipenetrans, and Xiphinema index), the latter inc1udes various species of the Mermithidae family, and also the genera Steinernema and Helerorhabditis. There are few full-time nematologists in the country, and they work on taxonomy, distribution, host-parasite relationships, control, and different aspects of the biology of the major species. Due tO the importance of these organisms and the scarcity of information existing in Argentina about them, nematology can be considered a promising field for basic and applied research. Résumé - Les nématodes et l'agriculture en Argentine. Un aperçu général - Les nématodes du sol représentent en Argentine un groupe très diversifiè. Ayant une vaste répartition géographique, il comprend actuellement plus de deux cents espèces, celles parasitant les plantes et les insectes étant considèrées comme les plus importantes. Les espèces du genre Me/oi dogyne, ainsi que Nacobbus aberrans, Dùylenchus dipsaci, Tylenchulus semipenetrans et Xiphinema index représentent un réel danger pour certaines cultures. -
Burrowing Nematode Radopholus Similis (Cobb, 1893) Thorne, 1949 (Nematoda: Secernentea: Tylenchida: Pratylenchidae: Pratylenchinae)1 Nicholas Sekora and William T
EENY-542 Burrowing Nematode Radopholus similis (Cobb, 1893) Thorne, 1949 (Nematoda: Secernentea: Tylenchida: Pratylenchidae: Pratylenchinae)1 Nicholas Sekora and William T. Crow2 Introduction by fine textured soils rich in organic matter. However, soil texture plays a less important role on nematode population Radopholus similis, the burrowing nematode, is the most levels on banana (O’Bannon 1977). economically important nematode parasite of banana in the world. Infection by burrowing nematode causes toppling disease of banana, yellows disease of pepper and spreading Life Cycle and Biology decline of citrus. These diseases are the result of burrowing Burrowing nematode is an endoparasitic migratory nema- nematode infection destroying root tissue, leaving plants tode, meaning it completes its life cycle within root tissue. with little to no support or ability to take up water and All motile juvenile stages and females can infect root tissue translocate nutrients. Because of the damage that it causes at any point along the length of a root. After root penetra- to citrus, ornamentals and other agricultural industries, tion, these life stages mainly feed and migrate into the worldwide, burrowing nematode is one of the most regu- cortical parenchyma and also into the stele. Mature males lated nematode plant pests (Hockland et al. 2006). of burrowing nematode are not infective. As the mature females migrate through root tissue, they lay eggs that are Distribution produced through either sexual reproduction with males or by hermaphroditistim (Thorne 1961, Kaplan and Burrowing nematode is native to Australasia, but is found worldwide in tropical and subtropical regions of Africa, Opperman 2000). Once an egg hatches, the emergent Asia, Australia, North and South America, and many second-stage juvenile can migrate within the root and island regions. -
Theory Manual Course No. Pl. Path
NAVSARI AGRICULTURAL UNIVERSITY Theory Manual INTRODUCTORY PLANT NEMATOLOGY Course No. Pl. Path 2.2 (V Dean’s) nd 2 Semester B.Sc. (Hons.) Agri. PROF.R.R.PATEL, ASSISTANT PROFESSOR Dr.D.M.PATHAK, ASSOCIATE PROFESSOR Dr.R.R.WAGHUNDE, ASSISTANT PROFESSOR DEPARTMENT OF PLANT PATHOLOGY COLLEGE OF AGRICULTURE NAVSARI AGRICULTURAL UNIVERSITY BHARUCH 392012 1 GENERAL INTRODUCTION What are the nematodes? Nematodes are belongs to animal kingdom, they are triploblastic, unsegmented, bilateral symmetrical, pseudocoelomateandhaving well developed reproductive, nervous, excretoryand digestive system where as the circulatory and respiratory systems are absent but govern by the pseudocoelomic fluid. Plant Nematology: Nematology is a science deals with the study of morphology, taxonomy, classification, biology, symptomatology and management of {plant pathogenic} nematode (PPN). The word nematode is made up of two Greek words, Nema means thread like and eidos means form. The words Nematodes is derived from Greek words ‘Nema+oides’ meaning „Thread + form‟(thread like organism ) therefore, they also called threadworms. They are also known as roundworms because nematode body tubular is shape. The movement (serpentine) of nematodes like eel (marine fish), so also called them eelworm in U.K. and Nema in U.S.A. Roundworms by Zoologist Nematodes are a diverse group of organisms, which are found in many different environments. Approximately 50% of known nematode species are marine, 25% are free-living species found in soil or freshwater, 15% are parasites of animals, and 10% of known nematode species are parasites of plants (see figure at left). The study of nematodes has traditionally been viewed as three separate disciplines: (1) Helminthology dealing with the study of nematodes and other worms parasitic in vertebrates (mainly those of importance to human and veterinary medicine). -
Silencing Parasitism Effectors of the Root Lesion Nematode, Pratylenchus Thornei
Silencing parasitism effectors of the root lesion nematode, Pratylenchus thornei. This thesis is presented for the degree of Doctor of Philosophy of Murdoch University by Sameer Dilip Khot B.Sc. (Botany) & M.Sc. (Plant Pathology & Mycology), University of Mumbai, India M.S. (Plant Pathology), North Dakota State University, USA Western Australian State Agricultural Biotechnology Centre School of Veterinary and Life Sciences Murdoch University Perth, Western Australia 2018 1 DECLARATION I declare that this thesis is my own account of my research and contains as its main content work which has not previously been submitted for a degree at any tertiary education institution. Signature: Sameer D. Khot Date: 22-01-2018 2 ABSTRACT The root lesion nematode (RLN), Pratylenchus thornei, is a biotrophic migratory pest of plant roots and its infestation causes losses in many economically important crops. RNA interference (RNAi) is a naturally occurring eukaryotic phenomenon and can be used to silence parasitism effector genes of P. thornei using host-mediated RNAi. This may be developed as an environmentally friendly and a cost-effective control strategy. The overall aims of this research were to investigate the effects of in vitro and in planta RNAi silencing of putative P. thornei parasitism effector genes, and their nematicidal effects in two host plants. Five putative target parasitism genes vital for nematode entry into roots (Pt-Eng-1, Pt- PL), feeding (Pt-CLP) and suppressing host defence responses (Pt-UEP, Pt-GST) were identified, validated in silico using comparative bioinformatics, cloned into suitable in vitro transcription and binary vectors, and advanced to RNAi studies. -
ARTHROPODA Subphylum Hexapoda Protura, Springtails, Diplura, and Insects
NINE Phylum ARTHROPODA SUBPHYLUM HEXAPODA Protura, springtails, Diplura, and insects ROD P. MACFARLANE, PETER A. MADDISON, IAN G. ANDREW, JOCELYN A. BERRY, PETER M. JOHNS, ROBERT J. B. HOARE, MARIE-CLAUDE LARIVIÈRE, PENELOPE GREENSLADE, ROSA C. HENDERSON, COURTenaY N. SMITHERS, RicarDO L. PALMA, JOHN B. WARD, ROBERT L. C. PILGRIM, DaVID R. TOWNS, IAN McLELLAN, DAVID A. J. TEULON, TERRY R. HITCHINGS, VICTOR F. EASTOP, NICHOLAS A. MARTIN, MURRAY J. FLETCHER, MARLON A. W. STUFKENS, PAMELA J. DALE, Daniel BURCKHARDT, THOMAS R. BUCKLEY, STEVEN A. TREWICK defining feature of the Hexapoda, as the name suggests, is six legs. Also, the body comprises a head, thorax, and abdomen. The number A of abdominal segments varies, however; there are only six in the Collembola (springtails), 9–12 in the Protura, and 10 in the Diplura, whereas in all other hexapods there are strictly 11. Insects are now regarded as comprising only those hexapods with 11 abdominal segments. Whereas crustaceans are the dominant group of arthropods in the sea, hexapods prevail on land, in numbers and biomass. Altogether, the Hexapoda constitutes the most diverse group of animals – the estimated number of described species worldwide is just over 900,000, with the beetles (order Coleoptera) comprising more than a third of these. Today, the Hexapoda is considered to contain four classes – the Insecta, and the Protura, Collembola, and Diplura. The latter three classes were formerly allied with the insect orders Archaeognatha (jumping bristletails) and Thysanura (silverfish) as the insect subclass Apterygota (‘wingless’). The Apterygota is now regarded as an artificial assemblage (Bitsch & Bitsch 2000). -
Diversity, Phylogeny, Characterization and Diagnostics of Root-Knot and Lesion Nematodes
Diversity, phylogeny, characterization and diagnostics of root-knot and lesion nematodes Toon Janssen Promotors: Prof. Dr. Wim Bert Prof. Dr. Gerrit Karssen Thesis submitted to obtain the degree of doctor in Sciences, Biology Proefschrift voorgelegd tot het bekomen van de graad van doctor in de Wetenschappen, Biologie 1 Table of contents Acknowledgements Chapter 1: general introduction 1 Organisms under study: plant-parasitic nematodes .................................................... 11 1.1 Pratylenchus: root-lesion nematodes ..................................................................................... 13 1.2 Meloidogyne: root-knot nematodes ....................................................................................... 15 2 Economic importance ..................................................................................................... 17 3 Identification of plant-parasitic nematodes .................................................................. 19 4 Variability in reproduction strategies and genome evolution ..................................... 22 5 Aims .................................................................................................................................. 24 6 Outline of this study ........................................................................................................ 25 Chapter 2: Mitochondrial coding genome analysis of tropical root-knot nematodes (Meloidogyne) supports haplotype based diagnostics and reveals evidence of recent reticulate evolution. 1 Abstract -
Description of Pratylenchus Gutierrezi N. Sp. (Nematoda: Pratylenchidae
Journal of Nematology 24(2):298-304. 1992. © The Society of Nematologists 1992. Description of Pratylenchus 9utierrezi n. sp. (Nematoda: Pratylenchidae) from Coffee in Costa Rica A. MORGAN GOLDEN, 1 ROGER L6PEZ CH., 2 AND HERNAN VILCHEZ R. 2 Abstract: A lesion nematode, Pratylenchu6 gutierrezi n. sp., collected from the roots of coffee in the Central Plateau of Costa Rica, is described and illustrated. Its relationships to Pratylenchusflakkensis, P. similis, and P. gibbicaudatus, the only other species of the genus having two head annules, males, or spermatheca with sperm, and an annulated tail terminus, is discussed. Other distinctive characters are its posterior vulva (mean of 80%); its prominently rounded stylet knobs, low head, and subcy- lindrical tail. SEM observations provide additional details of females and males, especially face views, which show for the first time sexual dimorphism. Key words: Coffea arabica, Costa Rica, lesion nematode, morphology, nematode, new species, Pra- tylenchus flakkensis, P. gibbicaudatus, P. gutierrezi n. sp., P. similis, scanning electron microscopy, SEM, taxonomy. Certain species of lesion nematodes to be a new Pratylenchus species, which is (Pratylenchus spp.) are important parasites described and illustrated herein. of coffee (Coffea spp.). In a recent excellent review of nematodes reported to occur on MATERIALS AND METHODS coffee (1), the following five Pratylenchus species were listed: P. brachyurus (Godfrey, Nematodes were extracted from in- 1929) Filipjev & Schuurmans Stekhoven, fected coffee roots by placing chopped or 1941; P. coffeae (Zimmermann, 1889) Fil- blenderized roots on filter paper over wa- ipjev & Schuurmans Stekhoven, 1941; P. ter in a Baermann funnel. Specimens were goodeyi Sher & Alien, 1953; P. -
Abstracts 501-750
150 salivary glands was greatly reduced by ron2 silencing, despite sporogony, and accompanying upregulation of PfRad51, PfRad54, PfRPA1L and sporozoite release into hemocoel and their motility were normal. These PfRPA1S at the level of transcript and protein. This study provides new results showed that RON2 is required for salivary gland invasion. This is the insights into the role of putative Rad51-interacting proteins involved in first genetical approach to show that RON2 has an important role in target homologous recombination and emphasizes physiological role of DNA cell invasion. damage repair during the growth of parasites. We are now characterizing the recombiantion macromolecular complex which is likely to be important 499 in DNA damage and repair and validating molecular interactions between PfRad51 and its putative interacting partners. Besides understanding IDENTIFICATION AND CHARACTERIZATION OF A molecular machinery involved in DNA repair and recombination, we wish PLASMODIUM FALCIPARUM ORTHOLOGUE OF THE YEAST to extend our studies to understand the biochemical and genetic basis of UBIQUINONE-BINDING PROTEIN, COQ10P gene rearrangements at the var gene locus associated with phenomenon like antigenic variation. Bethany J. Jenkins, Joanne M. Morrisey, Thomas M. Daly, Michael W. Mather, Akhil B. Vaidya, Lawrence W. Bergman 501 Drexel University College of Medicine, Philadelphia, PA, United States Coenzyme Q (CoQ, ubiquinone) is a central electron carrier in A SINGLE NUCLEOTIDE POLYMORPHISM IN THE PROMOTER mitochondrial respiration. CoQ is synthesized through multiple steps OF STROMAL CELL-DERIVED FACTOR (SDF)-1α (C-1002T) IS involving a number of different proteins. The prevailing view that the ASSOCIATED WITH PROTECTION AGAINST PLASMODIUM CoQ used in respiration exists as a free pool that diffuses throughout FALCIPARUM INFECTION IN KENYAN CHILDREN the mitochondrial inner membrane bilayer has recently been challenged.