DOCSLIB.ORG

Abstracts 501-750

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Abstracts 501-750 150 salivary glands was greatly reduced by ron2 silencing, despite sporogony, and accompanying upregulation of PfRad51, PfRad54, PfRPA1L and sporozoite release into hemocoel and their motility were normal. These PfRPA1S at the level of transcript and protein. This study provides new results showed that RON2 is required for salivary gland invasion. This is the insights into the role of putative Rad51-interacting proteins involved in first genetical approach to show that RON2 has an important role in target homologous recombination and emphasizes physiological role of DNA cell invasion. damage repair during the growth of parasites. We are now characterizing the recombiantion macromolecular complex which is likely to be important 499 in DNA damage and repair and validating molecular interactions between PfRad51 and its putative interacting partners. Besides understanding IDENTIFICATION AND CHARACTERIZATION OF A molecular machinery involved in DNA repair and recombination, we wish PLASMODIUM FALCIPARUM ORTHOLOGUE OF THE YEAST to extend our studies to understand the biochemical and genetic basis of UBIQUINONE-BINDING PROTEIN, COQ10P gene rearrangements at the var gene locus associated with phenomenon like antigenic variation. Bethany J. Jenkins, Joanne M. Morrisey, Thomas M. Daly, Michael W. Mather, Akhil B. Vaidya, Lawrence W. Bergman 501 Drexel University College of Medicine, Philadelphia, PA, United States Coenzyme Q (CoQ, ubiquinone) is a central electron carrier in A SINGLE NUCLEOTIDE POLYMORPHISM IN THE PROMOTER mitochondrial respiration. CoQ is synthesized through multiple steps OF STROMAL CELL-DERIVED FACTOR (SDF)-1α (C-1002T) IS involving a number of different proteins. The prevailing view that the ASSOCIATED WITH PROTECTION AGAINST PLASMODIUM CoQ used in respiration exists as a free pool that diffuses throughout FALCIPARUM INFECTION IN KENYAN CHILDREN the mitochondrial inner membrane bilayer has recently been challenged. 1 1 1 2 In the yeast Saccharomyces cerevisiae, deletion of the gene encoding Grace Okello , Zachary Karim , Prakasha Kempaiah , Eric Otieno , 3 4 4 1 Coq10p results in respiration deficiency without altering total size of the James Hittner , John Vulule , John Ong’echa , Douglas Perkins , 4 available CoQ pool, suggesting that the Coq10p is critical for the delivery Tom Were of CoQ to the site(s) of respiration. The precise mechanism by which 1Center for Global Health - University of New Mexico, Albuquerque, this is achieved remains unknown at present. Because mitochondrial NM, United States, 2Laboratories of Parasitic and Viral Diseases, Centre respiration is a validated target for antimalarial drugs such as atovaquone, for Global Health Research, Kisumu, Kenya, 3Department of Psychology, we are interested in examining its regulation in malaria parasites. We have College of Charleston, Charleston, SC, United States, 4Centre for Global identified an orthologue of Coq10p, PfCoq10, in P. falciparum, the most Health Research, Kenya Medical Research Institute, Kisumu, Kenya virulent species of malaria parasite, and demonstrated that a GFP-tagged Stromal cell-derived factor (SDF)-1α (CXCL12) is a pleiotropic chemokine version of PfCoq10 localized to the parasite mitochondrion. Expression of with diverse functions including induction of anti-pathogen immunity PfCoq10 in the S. cerevisiae coq10 deletion strain restored the capability and inhibition of erythropoiesis. In murine malaria, increased expression of the yeast to grow on respiratory substrates, suggesting a remarkable of SDF-1α promotes control of parasitemia. Although several studies functional conservation of this protein over a vast evolutionary distance, indicate that SDF1A genetic variation regulates outcomes in the context and despite a relatively low level of amino acid sequence identity. We are of HIV-1 infection, hematopoiesis, and cancer, the role of genetic currently assessing effects of PfCoq10 overexpression on the atovaquone variability in SDF1A in Plasmodium falciparum infections has not been sensitivity of P. falciparum. We are also examining the possibility of altered explored. The effect of SDF1A (C-1002T, rs2839686) variation was response to atovaquone in yeast mitochondria expressing the parasite therefore, investigated in Kenyan children (2.0-38.0mos., n=873) residing Coq10. These studies may provide insights into respiration regulation in in a holoendemic P. falciparum transmission region of western Kenya. general, as well as in malaria parasites. Children were stratified into aparasitemic (n=212) and parasitemic (n=661) groups with parasitemic children being further categorized into SMA 500 (hemoglobin, Hb<5.0g/dL; n=236) vs. non-SMA (Hb≥5.0g/dL; n=425), high-density parasitemia (HDP; ≥10,000 parasites/µL; n=477) vs. low- ROLE OF PFRAD54 AND REPLICATION PROTEIN A IN RAD51- density parasitemia (LDP; <10,000 parasites/µL; n=184), and reticulocyte MEDIATED DNA STRAND EXCHANGE AND REPAIR OF DNA production index (RPI<2.0) vs. (RPI≥2.0). Multivariate logistic regression DAMAGE INDUCED BY MMS IN PLASMODIUM FALCIPARUM modeling controlling for age, gender, bacteremia, glucose-6-phosphate dehydrogenase, alpha-thalasemia, and sickle cell and HIV-1 status did not Anusha M. Gopalakrishnan, Nirbhay Kumar show any significant associations between carriage of C-1002T genotypes Tulane University School of Public Health, New Orleans, LA, United States and SMA, RPI<2.0, and HDP. However, carriage of the CC genotype was Exploiting the recombination machinery and its molecular characterization associated with protection against the acquisition of P. falciparum infection in the malaria parasite would provide mechanistic understanding of compared to the TT genotype (Odds ratio, OR, 0.311; 95% CI, 0.115- recombinational rearrangements leading to immune evasion via antigenic 0.842; P=0.022). These results demonstrate that although variation at switching, a major impediment in developing an effective vaccine against 1002 in the SDF1A promoter appears to protect against acquisition of P. these protozoan parasites. Bacterial RecA protein and its eukaryotic falciparum infection, this variant may not affect malaria disease outcomes homologue Rad51 play a central role in homologous DNA strand once an individual becomes infected. exchange reaction during recombination and DNA repair. Previously, our lab has shown that PfRad51, the Plasmodium falciparum homologue of 502 Rad51, exhibited ATPase activity and promoted DNA strand exchange in vitro, as reported previously. Here, we evaluated the catalytic functions GENOMIC DIVERSITY AND evolutionary history OF of PfRad51 in the presence of putative interacting partners, especially PLASMODIUM vivax P. falciparum homologues of Rad54 and Replication protein-A (RPA). Ernest R. Chan1, Didier Menard2, Odile Mercereau-Puijalon3, PfRad54 accelerated PfRad51 mediated pairing between ssDNA and its Peter Zimmerman4, David Serre1 homologous linear dsDNA in the presence of 0.5mM CaCl2. We also present evidence that recombinant PfRPA1L protein serves the function 1Cleveland Clinic Foundation, Cleveland, OH, United States, 2Institut of bacterial homologue SSB in initiating homologous pairing and strand Pasteur du Cambodge, Phnom Penh, Cambodia, 3Institut Pasteur, Paris, exchange activity but its function was negatively regulated in a dose- France, 4Case Western Reserve University, Cleveland, OH, United States dependent manner by PfRPA1S, another RPA homologue in P. falciparum. Most studies of genetic diversity in Plasmodium vivax have focused We also present in vivo evidence through comet assays for methyl on microsatellites or selected loci and do not provide a genome-wide methanesulfonate (MMS)- induced DNA damage in malaria parasites perspective. We have sequenced the genomes of ten P. vivax field isolates www.astmh.org 151 collected across the world, and three monkey-adapted strains. We 504 sequenced each sample on one lane of an Illumina HiSeq 2000 to obtain 70-400X coverage and have >93% of the reference genome covered by TRACKING CHANGING POPULATION DYNAMICS OF >20 reads. We conservatively identified >85,000 SNPs across the genome PLASMODIUM FALCIPARUM INFECTIONS REVEALS EVIDENCE as well as several sequence rearrangements. The genetic diversity is OF CLONAL INFECTIONS FOLLOWING INTRODUCTION significantly higher in intergenic regions than in coding regions (6.84 vs. OF TRANSMISSION-REDUCING INTERVENTIONS IN THIÉS, 3.24 SNPs/kb) with intronic sequences harboring an intermediate level SENEGAL (4.63 SNPs/kb). We also observed 1.5-fold more non-synonymous than synonymous SNPs while, based on the genome composition, we would Rachel Daniels1, Hsiao-Han Chang2, Papa Diogoye Séne3, Daniel expect 4-fold more nsSNPs. These observations suggest that evolution of Park2, Jimmy Vareta4, Danny A. Milner5, Daria van Tyne1, Terrie E. most P. vivax protein-coding sequences is driven by purifying selection. To Taylor4, Souleymane Mboup3, Daouda Ndiaye3, Dyann F. Wirth1, identify genes evolving under positive selection, we compared the rates of Daniel L. Hartl6, Sarah K. Volkman1 non-synonymous (PN) and synonymous substitutions (PS) for all protein- 1Harvard School of Public Health, Boston, MA, United States, 2Harvard coding sequences. Two red blood cell binding protein genes, the Duffy University, Cambridge, MA, United States, 3Cheikh Anta Diop University, binding protein and the reticulocyte binding protein 2 like, were among Dakar, Senegal, 4University of Malawi College of Medicine, Blantyre, the ten genes with the highest PN/PS ratio
Load more

Recommended publications
  • 4Th National IPM Symposium
    contents Foreword . 2 Program Schedule . 4 National Roadmap for Integrated Pest Management (IPM) . 9 Whole Systems Thinking Applied to IPM . 12 Fourth National IPM Symposium . 14 Poster Abstracts . 30 Poster Author Index . 92 1 foreword Welcome to the Fourth National Integrated Pest Management The Second National IPM Symposium followed the theme “IPM Symposium, “Building Alliances for the Future of IPM.” As IPM Programs for the 21st Century: Food Safety and Environmental adoption continues to increase, challenges facing the IPM systems’ Stewardship.” The meeting explored the future of IPM and its role approach to pest management also expand. The IPM community in reducing environmental problems; ensuring a safe, healthy, has responded to new challenges by developing appropriate plentiful food supply; and promoting a sustainable agriculture. The technologies to meet the changing needs of IPM stakeholders. meeting was organized with poster sessions and workshops covering 22 topic areas that provided numerous opportunities for Organization of the Fourth National Integrated Pest Management participants to share ideas across disciplines, agencies, and Symposium was initiated at the annual meeting of the National affiliations. More than 600 people attended the Second National IPM Committee, ESCOP/ECOP Pest Management Strategies IPM Symposium. Based on written and oral comments, the Subcommittee held in Washington, DC, in September 2001. With symposium was a very useful, stimulating, and exciting experi- the 2000 goal for IPM adoption having passed, it was agreed that ence. it was again time for the IPM community, in its broadest sense, to come together to review IPM achievements and to discuss visions The Third National IPM Symposium shared two themes, “Putting for how IPM could meet research, extension, and stakeholder Customers First” and “Assessing IPM Program Impacts.” These needs.
    [Show full text]
  • SIP Newsletter 2015 June V4.Pages
    Society for Invertebrate Pathology Newsletter Volume 48 Issue 2 June, 2015 Downtown Vancouver at Sunset. Photo Credit: Magnus3D Meeting Events: Saturday Tuesday Registration (2 pm - 8 pm) Concurrent Sessions Sunday Excursions and 5K Race BBQ at the Cheakamus Center SIP Council Meeting OECD Satellite Symposium Wednesday Bacteria Workshop Concurrent Sessions Opening Mixer Posters Monday Division Business Meetings Founders’ Lecture Thursday Plenary Symposium Concurrent Sessions Concurrent Sessions SIP Annual & Student Business Division Business Meetings Meetings Award Ceremonies and Banquet !1 From the President Dear SIP Colleagues, This communiqué is threefold. First, I would like to encourage those of you President who have yet to do so to register for the Peter Krell, Canada 2015 SIP in Vancouver Canada, second, convince those with a flair for Vice President writing to step up to replace Eric Haas Johannes Jehle, Germany Stapleton as SIP Newsletter Editor and Past President third, inform you about our Golden Jørgen Eilenberg, Denmark Jubilee Committee. The 48th SIP meeting is just around Secretary the corner, August 9 to 13, all in the Mary Barbercheck, USA newly opened “The Nest” at the beautiful University of British Treasurer Columbia campus, overlooking the Strait of Georgia between Stefan Jaronski, USA Vancouver and Vancouver Island, and only a short bicycle ride of about 90 miles (150 km) north of Seattle. There are many reasons Trustees to attend, just check out the meeting’s website on the SIP home Surendra Dara, USA Albrecht Koppenhofer, USA page. Famous for its natural beauty with great opportunities for Ed Lewis, USA hiking, canoeing and nature photography, along with both classical Monique van Oers, The Netherlands and aboriginal culture with a mixed east/west cuisine.
    [Show full text]
  • WO 2014/134709 Al 12 September 2014 (12.09.2014) P O P C T
    (12) INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau (10) International Publication Number (43) International Publication Date WO 2014/134709 Al 12 September 2014 (12.09.2014) P O P C T (51) International Patent Classification: (81) Designated States (unless otherwise indicated, for every A61K 31/05 (2006.01) A61P 31/02 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (21) International Application Number: BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DK, DM, PCT/CA20 14/000 174 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, (22) International Filing Date: HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, 4 March 2014 (04.03.2014) KZ, LA, LC, LK, LR, LS, LT, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, (25) Filing Language: English OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, (26) Publication Language: English SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, (30) Priority Data: ZW. 13/790,91 1 8 March 2013 (08.03.2013) US (84) Designated States (unless otherwise indicated, for every (71) Applicant: LABORATOIRE M2 [CA/CA]; 4005-A, rue kind of regional protection available): ARIPO (BW, GH, de la Garlock, Sherbrooke, Quebec J1L 1W9 (CA). GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, (72) Inventors: LEMIRE, Gaetan; 6505, rue de la fougere, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, Sherbrooke, Quebec JIN 3W3 (CA).
    [Show full text]
  • Skin and Soft Tissue Infections Ohsuerin Bonura, MD, MCR Oregon Health & Science University Objectives
    Difficult Skin and Soft tissue Infections OHSUErin Bonura, MD, MCR Oregon Health & Science University Objectives • Compare and contrast the epidemiology and clinical presentation of common skin and soft tissue diseases • State the management for skin and soft tissue infections OHSU• Differentiate true infection from infectious disease mimics of the skin Casey Casey is a 2 year old boy who presents with this rash. What is the best treatment? A. Soap and Water B. Ibuprofen, it will self OHSUresolve C. Dicloxacillin D. Mupirocin OHSUImpetigo Impetigo Epidemiology and Treatment OHSU Ellen Ellen is a 54 year old morbidly obese woman with DM, HTN and venous stasis who presented with a painful left leg and fever. She has had 3 episodes in the last 6 months. What do you recommend? A. Cefazolin followed by oral amoxicillin prophylaxis B. Vancomycin – this is likely OHSUMRSA C. Amoxicillin – this is likely erysipelas D. Clindamycin to cover staph and strep cellulitis Impetigo OHSUErysipelas Erysipelas Risk: lymphedema, stasis, obesity, paresis, DM, ETOH OHSURecurrence rate: 30% in 3 yrs Treatment: Penicillin Impetigo Erysipelas OHSUCellulitis Cellulitis • DEEPER than erysipelas • Microbiology: – 6-48hrs post op: think GAS… too early for staph (days in the making)! – Periorbital – Staph, Strep pneumoniae, GAS OHSU– Post Varicella - GAS – Skin popping – Staph + almost anything! Framework for Skin and Soft Tissue Infections (SSTIs) NONPurulent Purulent Necrotizing/Cellulitis/Erysipelas Furuncle/Carbuncle/Abscess Severe Moderate Mild Severe Moderate Mild I&D I&D I&D I&D IV Rx Oral Rx C&S C&S C&S C&S Vanc + Pip-tazo OHSUEmpiric IV Empiric MRSA Oral MRSA TMP/SMX Doxy What Are Your “Go-To” Oral Options For Non-Purulent SSTI? Amoxicillin Doxycycline OHSUCephalexin Doxycycline Trimethoprim-Sulfamethoxazole OHSU Miller LG, et al.
    [Show full text]
  • Antibiotic Use Guidelines for Companion Animal Practice (2Nd Edition) Iii
    ii Antibiotic Use Guidelines for Companion Animal Practice (2nd edition) iii Antibiotic Use Guidelines for Companion Animal Practice, 2nd edition Publisher: Companion Animal Group, Danish Veterinary Association, Peter Bangs Vej 30, 2000 Frederiksberg Authors of the guidelines: Lisbeth Rem Jessen (University of Copenhagen) Peter Damborg (University of Copenhagen) Anette Spohr (Evidensia Faxe Animal Hospital) Sandra Goericke-Pesch (University of Veterinary Medicine, Hannover) Rebecca Langhorn (University of Copenhagen) Geoffrey Houser (University of Copenhagen) Jakob Willesen (University of Copenhagen) Mette Schjærff (University of Copenhagen) Thomas Eriksen (University of Copenhagen) Tina Møller Sørensen (University of Copenhagen) Vibeke Frøkjær Jensen (DTU-VET) Flemming Obling (Greve) Luca Guardabassi (University of Copenhagen) Reproduction of extracts from these guidelines is only permitted in accordance with the agreement between the Ministry of Education and Copy-Dan. Danish copyright law restricts all other use without written permission of the publisher. Exception is granted for short excerpts for review purposes. iv Foreword The first edition of the Antibiotic Use Guidelines for Companion Animal Practice was published in autumn of 2012. The aim of the guidelines was to prevent increased antibiotic resistance. A questionnaire circulated to Danish veterinarians in 2015 (Jessen et al., DVT 10, 2016) indicated that the guidelines were well received, and particularly that active users had followed the recommendations. Despite a positive reception and the results of this survey, the actual quantity of antibiotics used is probably a better indicator of the effect of the first guidelines. Chapter two of these updated guidelines therefore details the pattern of developments in antibiotic use, as reported in DANMAP 2016 (www.danmap.org).
    [Show full text]
  • Gene Gain and Loss Events in Rickettsia and Orientia Species Kalliopi Georgiades1,2, Vicky Merhej1, Khalid El Karkouri1, Didier Raoult1, Pierre Pontarotti2*
    Georgiades et al. Biology Direct 2011, 6:6 http://www.biology-direct.com/content/6/1/6 RESEARCH Open Access Gene gain and loss events in Rickettsia and Orientia species Kalliopi Georgiades1,2, Vicky Merhej1, Khalid El Karkouri1, Didier Raoult1, Pierre Pontarotti2* Abstract Background: Genome degradation is an ongoing process in all members of the Rickettsiales order, which makes these bacterial species an excellent model for studying reductive evolution through interspecies variation in genome size and gene content. In this study, we evaluated the degree to which gene loss shaped the content of some Rickettsiales genomes. We shed light on the role played by horizontal gene transfers in the genome evolution of Rickettsiales. Results: Our phylogenomic tree, based on whole-genome content, presented a topology distinct from that of the whole core gene concatenated phylogenetic tree, suggesting that the gene repertoires involved have different evolutionary histories. Indeed, we present evidence for 3 possible horizontal gene transfer events from various organisms to Orientia and 6 to Rickettsia spp., while we also identified 3 possible horizontal gene transfer events from Rickettsia and Orientia to other bacteria. We found 17 putative genes in Rickettsia spp. that are probably the result of de novo gene creation; 2 of these genes appear to be functional. On the basis of these results, we were able to reconstruct the gene repertoires of “proto-Rickettsiales” and “proto-Rickettsiaceae”, which correspond to the ancestors of Rickettsiales and Rickettsiaceae, respectively. Finally, we found that 2,135 genes were lost during the evolution of the Rickettsiaceae to an intracellular lifestyle. Conclusions: Our phylogenetic analysis allowed us to track the gene gain and loss events occurring in bacterial genomes during their evolution from a free-living to an intracellular lifestyle.
    [Show full text]
  • Scrub Typhus and Molecular Characterization of Orientia Tsutsugamushi from Central Nepal
    pathogens Article Scrub Typhus and Molecular Characterization of Orientia tsutsugamushi from Central Nepal Rajendra Gautam 1, Keshab Parajuli 1, Mythili Tadepalli 2, Stephen Graves 2, John Stenos 2,* and Jeevan Bahadur Sherchand 1 1 Department of Microbiology, Maharajgunj Medical Campus, Institute of Medicine, Kathmandu 44600, Nepal; [email protected] (R.G.); [email protected] (K.P.); [email protected] (J.B.S.) 2 Australian Rickettsial Reference Laboratory, Geelong, VIC 3220, Australia; [email protected] (M.T.); [email protected] (S.G.) * Correspondence: [email protected]; Tel.: +61-342151357 Abstract: Scrub typhus is a vector-borne, acute febrile illness caused by Orientia tsutsugamushi. Scrub typhus continues to be an important but neglected tropical disease in Nepal. Information on this pathogen in Nepal is limited to serological surveys with little information available on molecular methods to detect O. tsutsugamushi. Limited information exists on the genetic diversity of this pathogen. A total of 282 blood samples were obtained from patients with suspected scrub typhus from central Nepal and 84 (30%) were positive for O. tsutsugamushi by 16S rRNA qPCR. Positive samples were further subjected to 56 kDa and 47 kDa molecular typing and molecularly compared to other O. tsutsugamushi strains. Phylogenetic analysis revealed that Nepalese O. tsutsugamushi strains largely cluster together and cluster away from other O. tsutsugamushi strains from Asia and elsewhere. One exception was the sample of Nepal_1, with its partial 56 kDa sequence clustering Citation: Gautam, R.; Parajuli, K.; more closely with non-Nepalese O. tsutsugamushi 56 kDa sequences, potentially indicating that Tadepalli, M.; Graves, S.; Stenos, J.; homologous recombination may influence the genetic diversity of strains in this region.
    [Show full text]
  • Pyoderma Gangrenosum in Myelodysplasia and Acute Leukaemia
    Postgrad Med J: first published as 10.1136/pgmj.61.718.689 on 1 August 1985. Downloaded from Postgraduate Medical Journal (1985) 61, 689-694 Pyoderma gangrenosum in myelodysplasia and acute leukaemia Peter Jacobs', S. Palmer2 and E.C. Gordon-Smith2 'The University ofCape Town Leukaemia Centre and the Department ofHaematology, Groote Schuur Hospital, Observatory, 7925, Cape Town, South Africa and 'The Department ofHaematology, Royal Postgraduate Medical School, London, UK. Summary: Pyoderma gangrenosum is a rare occurrence in patients with haematological malignancy. This characteristic but nonspecific inflammatory process with skin destruction occurred in 4 patients with myelodysplasia, in one with acute leukaemic transformation of myelofibrosis, and in de novo acute myeloblastic leukaemia in another. Clinically, the cutaneous lesion in these patients differed from that associated with inflammatory bowel disease, arthritis, or the idiopathic type ofpyoderma gangrenosum by having the vesiculo-bulious borders. Histopathological differences were also evident since more superficial layers ofthe skin were involved in the ulceration than typically encountered in patients with non-malignant systemic disease. Despite the less penetrating nature of this variant, treatment of the pyoderma gangrenosum is unsatisfactory and in the absence of effective therapy for the underlying disease, healing occurred only in the patient with acute leukaemia who achieved complete remission in response to chemotherapy. copyright. Introduction Pyoderma gangrenosum
    [Show full text]
  • Module Test № 1 on Dermatology
    THE MINISTRY OF HEALTHCARE OF THE RUSSIAN FEDERATION FEDERAL STATE BUDGETARY EDUCATIONAL INSTITUTION OF HIGHER PROFESSIONAL EDUCATION PIROGOV RUSSIAN NATIONAL RESEARCH MEDICAL UNIVERSITY DEPARTMENT OF DERMATOVENEROLOGY Gaydina T.A., Dvornikov A.S., Skripkina P.A., Nazhmutdinova D.K., Heydar S.A., Arutunyan G.B., Pashinyan A.G. MODULE TEST №1 ON DERMATOLOGY FOR STUDENTS OF INSTITUTES OF HIGHER MEDICAL EDUCATION ON SPECIALTY THERAPEUTIC FACULTY DEPARTMENT OF DERMATOVENEROLOGY Moscow 2016 ISBN УДК ББК A21 Module test №1 on Dermatology for students of institutes of high medical education on specialty «Therapeutic faculty» department of dermatovenerology: manual for students for self-training//FSBEI HPE “Pirogov RNRMU” of the ministry of healthcare of the russian federation, M.: (publisher) 2016, 144 p. The manual is a part of teaching-methods on Dermatovenerology. It contains tests on Dermatology on the topics of practical sessions requiring single or multiple choice anser. The manual can be used to develop skills of students during practical sessions. It also can be used in the electronic version at testing for knowledge. The manual is compiled according to FSES on specialty “therapeutic faculty”, working programs on dermatovenerology. The manual is intended for foreign students of 3-4 courses on specialty “therapeutic faculty” and physicians for professional retraining. Authors: Gaydina T.A. – candidate of medical science, assistant of dermatovenerology department of therapeutic faculty Pirogov RNRMU Dvornikov A.S. – M.D., professor of dermatovenerology department of therapeutic faculty Pirogov RNRMU Skripkina P.A. – candidate of medical science, assistant professor of dermatovenerology department of therapeutic faculty Pirogov RNRMU Nazhmutdinova D.K. – candidate of medical science, assistant professor of dermatovenerology department of therapeutic faculty Pirogov RNRMU Heydar S.A.
    [Show full text]
  • Phylogenetic Analysis of Nematodes of the Genus Pratylenchus Using Nuclear 26S Rdna
    University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Faculty Publications from the Harold W. Manter Laboratory of Parasitology Parasitology, Harold W. Manter Laboratory of February 1997 Phylogenetic Analysis of Nematodes of the Genus Pratylenchus Using Nuclear 26S rDNA Luma Al-Banna University of Jordan, [email protected] Valerie M. Williamson University of California, Davis, [email protected] Scott Lyell Gardner University of Nebraska - Lincoln, [email protected] Follow this and additional works at: https://digitalcommons.unl.edu/parasitologyfacpubs Part of the Parasitology Commons Al-Banna, Luma; Williamson, Valerie M.; and Gardner, Scott Lyell, "Phylogenetic Analysis of Nematodes of the Genus Pratylenchus Using Nuclear 26S rDNA" (1997). Faculty Publications from the Harold W. Manter Laboratory of Parasitology. 52. https://digitalcommons.unl.edu/parasitologyfacpubs/52 This Article is brought to you for free and open access by the Parasitology, Harold W. Manter Laboratory of at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Faculty Publications from the Harold W. Manter Laboratory of Parasitology by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. Published in Molecular Phylogenetics and Evolution (ISSN: 1055-7903), vol. 7, no. 1 (February 1997): 94-102. Article no. FY960381. Copyright 1997, Academic Press. Used by permission. Phylogenetic Analysis of Nematodes of the Genus Pratylenchus Using Nuclear 26S rDNA Luma Al-Banna*, Valerie Williamson*, and Scott Lyell Gardner1 *Department of Nematology, University of California at Davis, Davis, California 95676-8668 1H. W. Manter Laboratory, Division of Parasitology, University of Nebraska State Museum, W-529 Nebraska Hall, University of Nebraska-Lincoln, Lincoln, NE 68588-0514; [email protected] Fax: (402) 472-8949.
    [Show full text]
  • Persistence of Orientia Tsutsugamushiin Humans
    ORIGINAL ARTICLE Infectious Diseases, Microbiology & Parasitology http://dx.doi.org/10.3346/jkms.2012.27.3.231 • J Korean Med Sci 2012; 27: 231-235 Persistence of Orientia tsutsugamushi in Humans Moon-Hyun Chung1, Jin-Soo Lee1, We investigated the persistence of viable Orientia tsutsugamushi in patients who had Ji-hyeon Baek1, Mijeong Kim1, recovered from scrub typhus. Blood specimens were available from six patients with scrub and Jae-Seung Kang2 typhus who were at 1 to 18 months after the onset of the illness. The EDTA-treated blood specimens were inoculated into ECV304 cells, and cultures were maintained for 7 months. 1Departments of Internal Medicine, and 2Microbiology, Inha University School of Sequencing of the 56-kDa type-specific antigen gene ofO. tsutsugamushi was performed Medicine, Incheon, Korea to ascertain the homology of isolates. O. tsutsugamushi was isolated from all six patients, and nucleotide sequences of isolates serially collected from each patient were identical in Received: 9 October 2011 all five patients in whom nucleotide sequences were compared. One patient relapsed 2 Accepted: 3 January 2012 days after completion of antibiotic therapy; two patients complained of weakness for 1 to Address for Correspondence: 2.5 months after the illness; one patient underwent coronary angioplasty 6 months later; Jae-Seung Kang, MD and one patient suffered from a transient ischemic attack 8 months later. This finding Department of Microbiology, Inha University School of Medicine, 27 Inhang-ro, Jung-gu, Incheon 400-712, Korea suggests that O. tsutsugamushi causes chronic latent infection, which may be associated Tel: +82.32-890-0952, Fax: +82.32-881-8559 with certain clinical illnesses, preceded by scrub typhus.
    [Show full text]
  • Superficial Pyoderma
    W2825-Ch003.qxd 8/4/2005 13:33 Page 34 34 CHAPTER 3 Bacterial Skin Diseases Superficial Pyoderma (superficial bacterial folliculitis) Features emerging as a common canine isolate in patients with Superficial pyoderma is a superficial bacterial infection chronic infections and previous antibiotic exposure. Ad- involving hair follicles and the adjacent epidermis. The ditionally, methicillin-resistant Staphylococcus aureus infection usually occurs secondary to an underlying (human MRSA) may be becoming more common cause; allergies and endocrine disease are the most among veterinary species. common causes (Box 3-3). Superficial pyoderma is common in dogs and rare in cats. Superficial pyoderma is characterized by focal, mul- Top Differentials tifocal, or generalized areas of papules, pustules, Differentials include demodicosis, dermatophytosis, crusts, and scales, epidermal collarettes, or circum- scabies, and autoimmume skin diseases. scribed areas of erythema and alopecia that may have hyperpigmented centers. Short-coated dogs often present with a “moth-eaten” patchy alopecia, small Diagnosis tufts of hair that stand up, or reddish brown discol- 1. Rule out other differentials oration of white hairs. In long-coated dogs, symptoms 2. Cytology (pustule): neutrophils and bacterial cocci can be insidious and may include a dull, lusterless hair 3. Dermatohistopathology: epidermal microabscesses, coat, scales, and excessive shedding. In both short- and nonspecific superficial dermatitis, perifolliculitis, long-coated breeds, primary skin lesions are often and folliculitis. Intralesional bacteria may be diffi- obscured by remaining hairs but can be readily appre- cult to find ciated if an affected area is clipped. Pruritus is variable, 4. Bacterial culture: Staphylococcus species ranging from none to intense levels.
    [Show full text]