Product Instructions Photonmaster™ Luminometer & Bluetooth® Module Product #: EQP-PMT and EQP-PBM

Product Instructions PhotonMaster™ Luminometer & Bluetooth® Module Product #: EQP-PMT and EQP-PBM

Product Overview

The ATP analysis harnesses a naturally-occurring, light- producing process to measure total microorganism concentration. The amount of light produced in the analysis is directly related to the quantity of microorganisms. To measure the light intensity and therefore microbiological activity, a luminometer is required. LuminUltra supplies the PhotonMaster Luminometer (EQP- PMT) for this purpose. As a USB-operated and powered device, the PhotonMaster requires an external device to operate. While users can directly connect to a Windows- based PC for this purpose, the PhotonMaster Bluetooth Module (EQP-PBM ) allows users the additional options of stand-alone offline operation through a keypad and screen, or pass-through operation on your mobile device via Bluetooth technology. Full integration with our LuminUltra Cloud™ software is available through any of these modes of operation so that you can get the most out of your data.

Product Contents

We recommend that every PhotonMaster Luminometer be stored and operated in its supplied carrying case, which also includes a space for the PhotonMaster Bluetooth Module and all the other equipment needed to carry out LuminUltra’s test protocols. Contents nor mally include: · PhotonMaster Luminometer c/w built-in USB cable; · PhotonMaster Bluetooth Module c/w 6’ USB charging cable; · Carrying case including tablet mounting system; · Micropipettors for reliable and accurate fluid transfer; · Test tube racks for both reagent tubes and luminometer cuvettes. This complete system can operate independently of LuminUltra Cloud software; however, for the best user experience visit www.luminultra.com/luminultra-cloud to download the mobile app on your Android or iOS device or use your computer & web browser to access the software through the web.

LuminUltra Technologies Ltd. Page 1 of 8 520 King Street Fredericton, New Brunswick, Canada E3B 6G3 Date Modified: 13-Jul-20 +1-506-459-8777 © LuminUltra 2019 www.luminultra.com

Product Instructions – EQP-PMT and EQP-PBM

Product Specifications

PhotonMaster Luminometer (EQP-PMT) Linear Dynamic Range (ATP): 4 x 10 -12 to 1 x 10 -6M ATP Linear Dynamic Range (RLU): 0 to 10,000,000 RLU

Assay Tube Chamber: f12 x 55mm Detector: Photomultiplier Tube (PMT) Interface: USB, 3ft cord Power: USB 5-volt <120mA Control Methods: PhotonMaster Bluetooth Module, LuminUltra Cloud software Dimensions (mm): 77W x 125D x 88H Weight: 1.01lbs (460g) Storage Temperature: -10 to +50°C Operating Temperature: +5 to +35°C

PhotonMaster Bluetooth Module (EQP-PBM) Battery: 2600 mAh Lithium-Ion (1500 readings on full charge) Luminometer USB Connection: Type A Female; 100mA output PC/Power USB Connection: Type-B Micro Female; 500mA input Bluetooth Connection: Bluetooth Low Energy (BLE), Bluetooth Classic (BTC) Control Methods: Onboard, USB-to-PC, Bluetooth-to-mobile Dimensions (mm): 130 x 70 x 26 (L x W x H) Weight: 152g Storage Temperature: -10 to +50°C Operating Temperature: +5 to +35°C Measurements: 1000+ measurements (from full charge) Low Battery Indicator: 5 measurements (red battery LED will start to blink) Memory: 1000 Sample Points (Notification to clear memory at 900 SP)

NOTE: The PhotonMaster is a sensitive laboratory device and while designed for field use, should be handled with care. Avoid operating the unit in excessive high or low temperatures, avoid operation in direct sunlight, and avoid exposure to physical shock. We recommend storing your PhotonMaster in its supplied field case at all times, especially during transportation.

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Product Instructions – EQP-PMT and EQP-PBM

PBM Charging & Connection Instructions

The PhotonMaster Bluetooth Module uses an integrated high capacity Li-ion battery delivering enough power to perform more than 1000 readings on a single charge with a PhotonMaster luminometer. When the PBM is in standby mode (turned off) it draws a small current to maintain operational functionality, which over time will deplete the battery. The PBM can be charged from most common USB wall chargers (not supplied) or from a USB port on a computer. A full charge will take approximately 5 hours to complete based on a PBM USB Micro Port maximum 500mA power supply (common found on phone chargers). Connect the Micro USB to USB cable (supplied) to the PBM port labelled ‘PC’ and the opposite end to a USB charger or USB port on a computer. With the PBM turned ON and the charging cable connected, the red battery indicator light PBM Battery Indicator will illuminate to signify the unit is being charged. The battery is fully charged when the battery indicator light does not illuminate with the PBM turned ON.

NOTE : When charging a device with a completely dead battery, an initial charge may be required before the PBM responds. In such cases it is advised to allow 10-15 minutes charge time to allow sufficient charge before the unit will power on.

Product Interface

The PhotonMaster luminometer is easy to use; simply plug in to the USB port of your PhotonMaster Bluetooth Module and start taking readings!

Top View & Button/LED Descriptions

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Product Instructions – EQP-PMT and EQP-PBM

User Interface Map

Cleaning Instructions

There are several routine maintenance steps that are recommended to ensure that the PhotonMaster Luminometer remains in good condition and that you continue to obtain the most accurate testing results.

If fluid has been spilled into the chamber , immediately follow these steps: 1. Remove assay tube – do not depress shutter or reclose the lid! 2. Keep lid open and invert the PhotonMaster. 3. Let the unit sit on a flat, clean surface for one hour. Do not expose PhotonMaster to bright light during this time, and do not perform these tasks in a dirty or dusty environment (e.g. outdoors). The number one cause of spillage in the PhotonMaster chamber is when assay tubes are left in the luminometer following testing. Please be sure to remove used assay tubes from the luminometer immediately upon completion of your testing.

LuminUltra recommends that the PhotonMaster be cleaned at least on an annual basis. Please use only the approved Luminometer Cleaning Kit (LCK) or Luminometer Maintenance Kit (LMK) that is furnished by LuminUltra, which includes 5 foam swabs and cleaning fluid. Never use sharp or abrasive materials for cleaning the PhotonMaster chamber, as this can compromise the sensitive optics of the device!

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Product Instructions – EQP-PMT and EQP-PBM

Disconnect your PhotonMaster from any USB connections before starting cleaning procedure.

1. Remove the rubber gasket from around the Counting Chamber.

2. Carefully lay the unit on its side with the lid open.

3. Using provided cleaning solution only; apply 6-8 drops to the swab. Using the dropper tip to spread the fluid around the swab to aid in absorption. Before inserting into the chamber.

4. Once cleaning fluid has thoroughly been absorbed by the swab, slowly insert into the chamber. Some fluid may be squeezed from the swab.

5. Insert the swab fully into the chamber until it stops. Turn the swab 6x clockwise while lifting the swab up and down within the chamber. Repeat 6x counter clockwise, lifting the swab up and down within the chamber to ensure complete coverage. If heavily soiled, apply more fluid and repeat steps.

DRYING : Using a new dry swab, repeat the 6x clockwise / counter clockwise motion. Pull the swab and feel for dampness. If damp, use a new swab and repeat twisting steps. Continue to use new clean swabs until the swab comes out dry. This usually requires three swabs. Verify that cleaning was successful by testing the travel of the piston in the chamber (it should be smooth) and through visual inspection. If there is still visible debris in the chamber, perform cleaning again.

NOTE: To prevent damage, do not reuse swabs after cleaning

Luminometer Linearity and Calibration Instructions The PhotonMaster does not require re-calibration, but LuminUltra does recommend that you verify linearity on an annual basis using our Luminometer Standardization Kit (LSK) or Luminometer Maintenance Kit (LMK) . For the best protection, we recommend that you clean your luminometer and verify linearity on an annual basis. Contact LuminUltra for details.

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Product Instructions – EQP-PMT and EQP-PBM

Handling Instructions

Please note some other handling instructions to consider when Operating your PhotonMaster Luminometer & Bluetooth Module:

PhotonMaster Luminometer

· Do not move or tilt the units during measurement and ensure you are working on a level surface. This may cause fluctuations in the measurement data. · Always remove the cuvette after taking a measurement to prevent liquid spilling inside the chamber and causing a malfunction with the PhotonMaster Luminometer. · Keep this unit at least 10 cm away from stirrers, mixers or any device that may generate electromagnetic noise. · Do not expose this unit to liquids, reagents or organic solvents. Doing so may cause malfunction, discoloration or deformation. If the unit was exposed to such liquids, reagents or solvents, then wipe them away immediately. · Avoid using this unit in locations: where the temperature fluctuates rapidly; directly exposed to steam; where excessive condensation occurs; where corrosive gases are generated; subject to vibration; with intense magnetic fields; or in locations exposed to excessive dust or debris. · Before using this unit, wash your hands thoroughly. This unit is extremely sensitive, so the ATP (Adenosine Triphosphate) may become contaminated through contamination from hands. · Do not leave this unit in unstable place or hazardous locations. Do not apply excessive shock to this unit and do not drop it. Doing so will cause permanent damage. · Be careful to prevent electrostatic charge buildup on the sample tube. Static electricity may cause an increase the measurement data.

PhotonMaster Bluetooth Module

· Do not leave this unit in unstable places or hazardous locations. Do not apply excessive shock to this unit and do not drop it. Doing so may cause permanent damage. · Do not expose this unit to excessive water or submerge. This unit is not water-proof, excessive water or moisture may cause an internal short or damage to the operation of the device. · Do not expose this unit to liquids, reagents or organic solvents. Doing so may cause malfunction, discoloration or deformation. If the unit was exposed to such liquids, reagents or solvents, then wipe them away immediately. · Avoid using this unit in locations: where the temperature fluctuates rapidly; directly exposed to steam; where excessive condensation occurs; where corrosive gases are generated; subject to vibration; with intense magnetic fields; or in locations exposed to excessive dust or debris. · Customers can return their used PBM for recycling by contacting us at www.luminultra.com and use our contact form. Please provide date of purchase and serial number of the unit you wish to dispose of and a representative will contact you within 1-2 business days with further instructions. Please do not return any items to us without prior written authorization.

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Product Instructions – EQP-PMT and EQP-PBM

Safety Precautions

The following warning symbols are used in this manual to designate the degree of hazard and damage that might occur if this product is used incorrectly.

Electrical shock hazard This symbol indicates a risk of electrical shock.

Fire hazard This symbol indicates a risk of smoke emission or fire.

Explosive hazard This symbol indicates a risk of explosion.

The following safety precautions should be taken and considered when operating the PhotonMaster Luminometer and PhotonMaster Bluetooth Module.

· If you notice abnormal operation such as a burning odor or smoke being emitted from this unit, then there is a risk of potential fire or internal explosion. After checking that the smoke has dissipated, please contact us or your local dealer. Never attempt to repair the problem yourself since this could be dangerous. Continued operation under abnormal conditions may lead to fire or electrical shock. · Do not use chemicals that may generate flammable gases and do not use in an atmosphere containing flammable gases. Doing so may cause gas explosion in this unit. · This unit contains high internal voltages, so improper handling may cause electrical shock, fire, or abnormal operation. · Never attempt to remove any cover of this unit or disassemble or repair any part of this product. Only our qualified service personnel are allowed to service this unit. This unit contains internal voltages, so improper handling may cause electrical shock, fire, or abnormal operation.

Warranty Information Your PhotonMaster luminometer and PhotonMaster Bluetooth module comes with a 1-year replacement warranty from date of purchase. To be eligible for this warranty, ensure that you fill out and return the warranty registration card provided with your PhotonMaster. Refer to this warranty form and statement for more information on the terms of this warranty.

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Product Instructions – EQP-PMT and EQP-PBM

Compliance Information The PhotonMaster Luminometer is CE and FCC compliant (per EMC Directive 2014/30/EU), RoHS compliant (per RoHS Directive 2011/65/EU and 2015/863), WEEE compliant, and conforms to C-Tick standards. The PhotonMaster Bluetooth Module is CE and FCC compliant, RoHS compliant (per RoHS Directive 2011/65/EU and 2015/863), WEEE Exempt (PBM not dependent on electricity to work properly in its basic (primary) function), and conforms to C-Tick standards. For more information, refer to the bottom sticker on the PhotonMaster Luminometer & Bluetooth Module or request a copy of the Declaration of Conformity from LuminUltra.

Ordering Information

New to 2 nd Generation ATP technology? Start by ordering the PhotonMaster & PBM Equipment Set and the test kit(s) of choice. Already own a PhotonMaster Luminometer and looking to upgrade your existing equipment to leverage the advantages of the PhotonMaster Bluetooth module? Equipment is available in the following formats:

For… Description Part # PhotonMaster & PBM Equipment Set · PhotonMaster Luminometer PhotonMaster Bluetooth Module New · · Equipment Case EQP-PBM-PAC Customer · Fixed volume Micropipettors · Test tube racks · Test Kit Tote Bag PhotonMaster & PBM New · PhotonMaster Luminometer EQP-PBM-PMT Customer · PhotonMaster Bluetooth Module · Equipment Case PhotonMaster - PBM Full Upgrade Kit · PhotonMaster Bluetooth Module · Equipment Case Upgrade EQP-PBM-UPGF · Fixed volume Micropipettors · Test tube racks · Test Kit Tote Bag PhotonMaster - PBM Basic Upgrade Kit Upgrade · PhotonMaster Bluetooth Module EQP-PBM-UPGB · Equipment Case NOTE: Please contact LuminUltra to inquire about purchasing individual components separately from the equipment sets above.

LuminUltra Technologies Ltd. 520 King Street Fredericton, New Brunswick Canada E3B 6G3 Tel: +1-506-459-8777 Fax: +1-506-453-9860 www.luminultra.com

To order: [email protected] For all other inquiries: [email protected]

Major credit cards (Visa, MasterCard, AMEX) are accepted. Contact LuminUltra by phone to place credit card orders. Orders generally ship within 3 business days. You will receive order confirmation via Email.

Lumitester is a trademark of Kikkoman Corporation. All other trademarks are the property of LuminUltra Technologies Ltd.

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Getting Started Guide 2nd Generation ATP Technology®

Introduction 2 LuminUltra Cloud Overview 3 Equipment Set Overview 4 Choosing the Right Test Kit 6 Receiving, Unpacking & Storing Materials 7 Product Use Guidelines 13 Technology Background 16 Ordering Terms & Conditions 20

LuminUltra Technologies Ltd. Page 1 of 20 520 King Street Fredericton, New Brunswick, Canada E3B 6G3 Date Modified: 30-Jan-17 +1-506-459-8777 www.luminultra.com © LuminUltra 2017 Introduction

At LuminUltra, we are committed to providing high quality test kits to anyone that needs fast and reliable results about the microbiological characteristics of any process! Visit www.luminultra.com to learn about the exciting opportunities that our solutions can provide.

In recognizing that our customers want complete solutions that fit their microbiological testing needs, we are pleased to offer a suite of products that together provide you with the most comprehensive package for proactive and accurate microbiological testing and control available on the market today. This solution comprises 4 parts:

SOFTWARE EQUIPMENT

In today’s fast-paced world, No matter what you are reacting quickly to process analyzing, if you do not have changes is the key to cost- and reliable and robust equipment, time-efficiency. LuminUltra your results will not be of good Cloud™ software will help you quality. LuminUltra is proud to to make real-time, reliable offer a suite of portable decisions and maximize the equipment, including its flagship value you gain from your data. PhotonMaster™ Luminometer & Bluetooth Module!

SERVICE TEST KITS

LuminUltra’s team of experts The cornerstone of LuminUltra’s can help you set up and solution is its line of 2 nd maintain an effective Generation ATP® test kits, microbiological control program which are the most complete tailored to your requirements. and reliable on the market today. We offer training, sample Our test kits are customized for analysis, test program setup, different applications, which and data analysis services. helps you to get the best results!

www.luminultra.com © LuminUltra 2017 Page 2 of 20 LuminUltra Cloud™ Overview

LuminUltra is proud to introduce LuminUltra Cloud ™, our ground breaking new software tool that delivers significant benefit for all customers using our test kits. When you’re in control of your data, you’re in control of your next decision.

View, edit and analyze data directly from your phone with LuminUltra Cloud - LuminUltra Cloud allows you to easily view and manipulate your data in convenient on-screen tables, as well as to manage alarms and decision support systems that identify when action is required.

Easily bring all of your data together - LuminUltra Cloud not only generates data wirelessly from your PhotonMaster (via our PhotonMaster Bluetooth Module, or PBM), but it also allows you to input other supplemental process data.

Your data can then be organized according to specific sites complete with GPS-tagged sample locations and set up scheduled workflows to share with your colleagues.

Discover insights at the speed of thought - LuminUltra Cloud includes several high-powered analytics, including line graphs and bar charts as well as advanced correlation tools using diagram overlays and Google Maps.

Analyze your data using powerful data treatments tools to reveal relationships between datasets, and display important analytics on your dashboard.

Save time generating reports - Our easy-to-use reporting module allows you to automatically email PDF reports to your colleagues.

All you need to do is set up the report content and recipient list, and we take care of the rest. Select from a wide array of report templates.

Secure, instantaneous sharing - LuminUltra Cloud is built upon best-in-class cloud database systems using secure and encrypted storage and data transfer.

This means that not only is your data sate, but collaboration with your colleagues is also real-time. Share sites along with your sampling programs, data, analytics, and notes with colleagues of your choosing by simply entering their email address.

Coming soon

LuminUltra Cloud will continue to grow and evolve over time to provide even more value to its customers. Some features to look out for in the future include quickly export data into a spreadsheet, import supplemental process data from a spreadsheet, email PDF reports on a schedule of your choosing, request your own custom report template, and much more!

Visit the LuminUltra Cloud product page at http://www.luminultra.com/luminultra-cloud/ for web links and app store download information.

LuminUltra also offers the LumiCalc and LumiCapture software platforms. Please contact us for additional details.

www.luminultra.com © LuminUltra 2017 Page 3 of 20 Equipment Set Overview

LuminUltra is pleased to offer a variety of equipment with which to utilize our revolutionary 2 nd Generation ATP test kits. Our different equipment offerings are built around different luminometers, which are highly-sensitive light detection devices required for the use of our test kits.

PhotonMaster Luminometer & Bluetooth Module

LuminUltra is proud to introduce the PhotonMaster, a purpose-built, low- cost luminometer specifically designed and optimized for LuminUltra’s patented line of 2 nd Generation ATP test kits. Our goal was to reduce equipment costs for our customers, but we didn’t stop there.

As the world’s first USB-powered and operated commercial-grade luminometer, the PhotonMaster has been designed to shield against the elements and potential interferences while working in typical field conditions. No need to worry about batteries or power cords; the PhotonMaster uses as little power as a typical computer mouse.

The PhotonMaster Blue Tooth Module allows users the additional options of stand-alone offline operation through a keypad and screen, or pass-through operation on your mobile device via Bluetooth technology! Full integration with our LuminUltra Cloud software is available through any of these modes of operation so that you can get the most out of your data.

While the PhotonMaster excels in its simplicity, the true power comes from combining our luminometer together with its control software, LuminUltra Cloud. The PhotonMaster detects light emitted from the bioluminescent reaction of our test kits using a photomultiplier tube. It then reports back in Relative Light Units (RLU) directly to our LuminUltra Cloud data analysis software which does the remaining work of calculating and interpreting the results for you!

LuminUltra also supports other luminometers such as the Kikkoman C-110 Lumitester. Please contact us for more information.

What’s in The Box?

What you receive in your equipment set will depend on what you ordered. Each of the above luminometers are available as stand-alone units supplied in rugged field cases, or as an equipment set that includes all of the equipment necessary to leverage the full power of our solutions!

www.luminultra.com © LuminUltra 2017 Page 4 of 20 Product Name & Description Catalogue # Appearance

PhotonMaster & PBM 1 x PhotonMaster Luminometer EQP-PBM-PMT 1 x PhotonMaster Bluetooth Module (EQP-PBM) 1 x Equipment Case (EQP-CASE-PBM)

PhotonMaster & PBM Equipment Set 1 x PhotonMaster Luminometer 1 x PhotonMaster Bluetooth Module (EQP-PBM) 1 x Equipment Case (EQP-CASE-PBM) 1 x Pipettor, Fixed Volume 0.1mL (EQP-SP100) EQP-PBM-PAC EQP-PBM-PAC 1 x Pipettor, Fixed Volume 0.3mL (EQP-SP300) 1 x Pipettor, Fixed Volume 1mL (EQP-SP1000) 1 x Test Tube Rack, Plastic, 12mm x 25 tube (EQP-T12-25) 2 x Test Tube Rack, 4-Way Flipper, Blue (EQP-T4) 1 x Test Kit Tote Bag (EQP-BAG)

More Information x The PhotonMaster is sold with a 1-year warranty. All other equipment is provided without any expressed warranty. Please remember to fill out and return your warranty card for optimum service! x Refer to the following documents for more information on these products:

o For specifications, equipment maintenance, and other information, please visit www.luminultra.com to download the individual Product Instructions for the PhotonMaster.

o The PhotonMaster is compliant with various global standards. For more information, request a copy of the Declaration of Conformity for either product from LuminUltra. x All of our luminometers comply with WEEE standards. Customers can return their used luminometer for recycling by contacting us at www.luminultra.com and use our contact form. Please provide date of purchase and serial number of the unit you wish to dispose of and a representative will contact you within 1-2 business days with further instructions. Please do not return any items to us without prior written authorization.

www.luminultra.com © LuminUltra 2017 Page 5 of 20 Choosing the Right Test Kit

So that you can rest easily on the quality of your data, LuminUltra supplies six standard test kits tailored for different sample types and applications. Visit our website at www.luminultra.com to determine the best test kit for your microbiological monitoring needs! All test kits are interchangeable with the equipment and software described earlier in this document.

I would like to test… Choose…

Low-Solids Water Samples Quench-Gone Aqueous (QGA) 9 Drinking Water 9 Cooling Water ASTM D4012 Compliant! 9 Sea Water 9 Purified Water

Low-Solids Organic and/or Water Mixtures Quench-Gone Organic Modified 9 Oilfield Applications (QGO-M) 9 Fuel & Associated Water ASTM D7687 & E2694 Compliant! 9 Metalworking Fluids & Lubricants 9 Oily Brines

High-Solids Process Waters QuenchGone21 Industrial (QG21I) 9 Papermaking Process Waters 9 Pulping Process Waters 9 Food Processing Slurries 9 Process Wash Waters

Manufactured Chemical Products QuenchGone21 Specialty (QG21S) 9 Paints, Coatings & Mineral Slurries 9 Adhesives, Admixtures & Polymers 9 Personal Care Products 9 Household & Industrial Care Products

Bioreactors & Associated Processes QuenchGone21 Wastewater 9 Aerobic & Anaerobic Processes (QG21W) 9 Activated Sludge, Lagoons & MBR’s 9 Raw Influent & Treated Effluent 9 Biosolids, Soils & Attached-growth Media

Attached / Sessile Microorganisms Deposit & Surface Analysis (DSA) 9 Production & Hygienic Surfaces 9 Biofilm Collection Devices 9 Corrosion Products & Slimes 9 Biologically-active Filter Media

For more information about these test kits, visit www.luminultra.com to obtain copies of the Test Kit Instructions for each individual test kit.

www.luminultra.com © LuminUltra 2017 Page 6 of 20 Receiving, Unpacking & Storing Materials

So you have received your order from LuminUltra. Now what? Simply follow these guidelines to get everything set up:

General Considerations x LuminUltra’s products are constructed to allow you the option to work virtually anywhere – in the lab, in your office, or in the field. x Wherever you choose to work, keep the following considerations in mind:

o Ensure that the environment in which you choose to work is relatively dust-free and clean.

o Avoid working in direct or intense sunlight. If you choose to work outside, ensure your workspace is in a shaded area.

o Avoid working in high-static areas, such as environments with extremely low humidity (e.g. 30% or less).

o An area of 3'x2' is generally sufficient for the purpose of performing ATP tests.

Equipment Set-up & Handling x When using luminometers, keep the following considerations in mind:

o Never operate luminometers in direct sunlight. While these instruments are built to be light-tight, inserting a cuvette into the chamber can expose the photomultiplier tube to sunlight which can temporarily cause high background RLUs.

o While most luminometers use a robust design to shield against electromagnetic fields, avoid operation near heavy machinery (e.g. pumps, blowers, etc.) or electrical panels.

o Always store and operate luminometers in a safe area where it cannot be bumped or dropped which can damage circuitry as well as the photomultiplier tube.

o Do not kink or bend AC and/or USB cords at a sharp angle.

o If liquid is spilled into the cuvette chamber, contact LuminUltra for guidance on cleaning. A specialized cleaning kit can be provided to you in situations such as this.

o Although luminometers do not require re-calibration during their useful life, you can confirm linearity and magnitude of response for your luminometer through the use of a Luminometer Standardization Kit (LSK) or a LuminUltra Maintenance Kit (LMK). Contact LuminUltra for more details. We recommend annual cleaning and linearity verification. x The following system specifications are required for operation of these luminometers and associated software:

o PhotonMaster : The PhotonMaster is incredibly easy to use; no special training is required to operate the unit. Simply plug in to the USB port of your PhotonMaster Bluetooth Module and start taking readings – it is that simple!

o PhotonMaster Bluetooth Module (PBM): The PBM is a handheld device that allows you to power your PhotonMaster luminometer and collect data anywhere in the field. Pair it with our LuminUltra Cloud app to complete your calculations, trend and share results, and send out reports to colleagues from any Bluetooth enabled mobile device. www.luminultra.com © LuminUltra 2017 Page 7 of 20 x All of our luminometers are supplied with a convenient and rugged field case that not only affords protection for the luminometer but also allows you to store all of the equipment required for the use of LuminUltra’s test kit. Refer to the following photos and points for a description of the equipment that can be stored in these field cases.

Portable Field Case Field Case & Equipment Micropipettors Use the convenient and rugged field case Store all of your equipment in the field All equipment sets come standard with to transport your equipment to where you case along with freeze-dried Luminase™ fixed-volume 0.1mL, 0.3mL and 1.0mL need it! reagents. pipettors.

4-Way Rack w/ 15mL & 50mL Tubes 12mm Test Tube Rack 4-Way Rack w/ 15mL Tubes Simply ‘flip’ the racks to hold different The 25-place test tube rack is provided to Two 4-way test tube racks are provided size tubes, such as 50mL centrifuge hold your assay tubes. for maximum convenience and flexibility. tubes!

Micropipetting Fundamentals

Micropipetting is an economical means of accurate fluid transfer in user-driven laboratory procedures. While LuminUltra equipment bundles include pipettors of appropriate sizes and designs for use with all LuminUltra test kits, nearly any micropipettor can be used so long as it is reliable and properly calibrated.

How do Micropipettors Work?

Most micropipettors operate using the same principal: a plunger draws liquid in and pushes it out. In some cases, the plunger is equipped with two stops: the 1 st being the calibrated stroke used to draw and discharge the correct volume and the 2 nd to blow out residual liquid.

Pipetting Tips & Tricks x When handling pipette tips, always hold tips by the large end. NEVER use a pipette tip that has contacted dirt, skin, or any other substance that could contaminate reagents or interfere with test results. x When using a tip with a particular liquid for the first time, flush the tip by drawing the desired volume into the tip, discharge, and then refill for use. x Always draw liquid into the tip slowly. If this is done too quickly, liquid could be sucked into the micropipettors or bubbles may form and occupy liquid space. x Dedicate pipette tips for one liquid only. Never pipette several different liquids with the same tip. x When drawing your sample using a micropipettor, ensure that the tip is securely fastened and that the tip is immersed into the sample for a sufficient period so that dripping does not occur. www.luminultra.com © LuminUltra 2017 Page 8 of 20 Operating Fixed-Volume Micropipettors

For your convenience, LuminUltra supplies fixed-volume micropipettors for the most rapid and economical yet accurate fluid transfer possible. Fixed-volume micropipettors are extremely easy to operate. Simply attach a fresh, unused pipet tip of the appropriate size and type to the pipettor. Then, depress the plunger, immerse the tip in your liquid, and allow the plunger to travel upwards. Move the pipettor to the location where you intend to dispense, and repeat this process.

Our fixed-volume micropipettors are designed to handle up to 1 year of normal use, after which time they should be disposed. If you suspect that these pipettors are underperforming or if you have been using them for longer than a year, contact LuminUltra to order new pipettors.

NOTE: These fixed volume micropipettors cannot be re-calibrated. Please do not loosen the collar holding in the piston on the pipet as it will result in an inaccurate volume being dispensed. If the collar becomes loose, please re- tighten it prior to use.

Operating Adjustable-Volume Micropipettors

Adjustable-volume micropipettors are an economical alternative to fixed-volume models when multiple volumes are required. Changing the pipettor volume is usually done by simply turning a dial, depending on the make or brand that is used. Figure 1 shows a typical volume adjustment.

The volume indicated depends on the size of the pipettor. For example, Cases A, B, and C above use a 100-ȝ/SLSHWWRUZKLOH&DVH'XVHVD -5mL pipettor. Comparing Cases B and D, the dial reading of “0450” is equivalent to two different values - ȝ/IRUWKH -ȝ/SLSHWWRUDQGP/ RUȝ/ RQWKH -5mL pipettor. Check the manufacturer’s literature if you are unsure how to make adjustments.

Detailed instructions for micropipettor operation are shown in Figure 1. For additional or equipment-specific details, consult your manufacturer’s instructions.

Figure 1 – Micropipettor Function Specific Instructions

www.luminultra.com © LuminUltra 2017 Page 9 of 20 Step 1 : Depress the plunger to the 1 st stop, then immerse the tip into the liquid.

Step 2 : For the rinse draw, slowly release the plunger to draw liquid into the tip.

Step 3 : Push the plunger to the 2 nd stop, pushing the fluid back into the vessel to rinse.

Step 4 : Allow the plunger to return to the top position.

Step 5 : For the calibrated draw, once again depress the plunger to the 1 st stop and immerse the tip into the liquid.

Step 6 : Slowly release the plunger to draw liquid into the tip.

Step 7 : Place the tip into the mouth of the receiving vessel.

Step 8 : Discharge the liquid by slowly pressing the plunger down to the 1 st stop.

Step 9 : Blow out the remaining liquid by pushing the plunger to the 2 nd stop

Step 10 : Release the plunger and discard the pipette tip.

NOTE: Adjustable volume micropipettors can be re-calibrated. Consult the manufacturer’s instructions for appropriate methods of re-calibration.

Setting up LuminUltra Cloud™ x To begin using LuminUltra Cloud, follow these basic steps:

o Go to https://www.luminultra.com/luminultra-cloud/ to find the weblinks to the Android and IOS app store.

o Download and install the Android or IOS application based on the type of phone you have in your possession.

o Open the app in your Android or IOS device and sign up for an account.

o Log in to the application and create a site.

o Pair your phone to the PhotonMaster Bluetooth Module.

o Start collecting data! x For further information on how to use the extensive functionality found in LuminUltra Cloud, refer to the built-in training materials.

www.luminultra.com © LuminUltra 2017 Page 10 of 20 Test Kit Handling x The composition of your test kit will vary depending on the type and size of the kit that you have ordered. Refer to the packing list and/or the test kit instructions for your test kit for a complete breakdown of its composition. x Instructions for the handling of plastic consumables found in your test kit:

o Locate a clean area to store pipette tips and test tubes. Keep the bags sealed with not in use to avoid contamination.

o All consumables in this test kit including pipet tips and test tubes are single-use only.

o Because ATP and bacteria are present on skin, do not to touch the surface of pipet tips. Ensure that all pipet tips and test tubes are clean inside and outside prior to use.

o Do not mark on assay tubes as this may impact light detection by the luminometer. x Instructions for the handling of reagents found in your test kit:

o As soon as possible after receipt of a test kit, open the box and remove the white vial box and handle Luminase reagents as follows:

There are multiple forms of Luminase reagents (in order from least active to most active: Luminase W, Luminase Lite , Luminase , and Luminase XL ). Despite their different activity levels, all version of Luminase are to be handled similarly and are the only parts of your test kit requiring special handling considerations. In general, the colder you store Luminase , the longer its useful life will be.

Luminase is manufactured using a process called freeze-drying. This maximizes product stability prior to use. Before using this product, it must first be rehydrated by mixing freeze-dried powder with liquid buffer and then allowed to incubate for at least 5 minutes. Take care to avoid contamination when removing the glass vial stopper.

Luminase Rehydration Process

Rehydrated Luminase can be stored in the refrigerator for up to 3 months (or freezer for up to 6 months with unlimited freeze-thaw cycles) following rehydration. Always bring cold rehydrated Luminase to ambient temperature prior to use. 1 hour is generally sufficient for this purpose.

Never expose rehydrated Luminase WR oC for longer than 1-2 hours.

In general, it is recommended that Luminase only be rehydrated as required. In other words, rehydrate on the day of testing rather than in advance.

www.luminultra.com © LuminUltra 2017 Page 11 of 20

Never attempt to partition portions of freeze-dried Luminase enzyme and/or the supplied buffer into smaller quantities.

If you begin utilizing a new bottle of Luminase during your testing, make sure to collect a new calibration result for that bottle. Alternatively, mix bottles of Luminase for all testing at one time.

Luminase is relatively temperature stable, so you can take it into the field for a few days to perform tests.

o Storage conditions and shelf life for all other reagents can be found on the product label and on the corresponding test kit instructions, and are generally room temperature storage.

o Caps on all reagent bottles and vials should be firmly sealed when not in use to avoid airborne contamination.

o Waste reagent can be discarded as general waste in most cases. Consult MSDS for more information. Contact LuminUltra for copies of MSDS. x You will find on certain reagent labels spaces for writing as follows:

o Pre-Dispensed Test Tubes – you are able to write the sample name on the label.

o Luminase Reagents – space is provided to note the date that the product was received so as to be able to estimate remaining reagent shelf life.

x With each LuminUltra equipment set, you will also receive a convenient Tote Bag for storage and transportation of your test kit components. Refer to the following photos for recommendations on how to use your tote bag.

Tote Bag & Test Kit Packing the Tote Bag Ready To Go! The supplied tote bag is designed to hold Pack everything you need including Together with the supplied equipment approximately 50 tests of any LuminUltra reagents and plastic consumables into field case, you are now ready to go to the test kit. the tote bag. field!

Product Use Guidelines

General Tips & Tricks x NEVER reuse pipette tips, tubes, etc. Use a new pipette tip for distributing each type of solution and a new tube for each new test. Ensure that all assay tubes are clean inside and outside. Do not mark on assay tubes as this may impact light detection by the luminometer. x Most unused plastic consumables are typically not contaminated and do not require sterilization. In spite of this, NEVER touch plastic consumables surfaces (since ATP and microorganisms are present on surfaces and skin) that will contact ATP reagents and protect surfaces from contaminants. x NEVER use reagents past their expiry date. x NEVER artificially settle samples (e.g. centrifuge) prior to testing. x Be wary of intense lighting and static charge in your work area. These actors can cause artificially-inflated RLU values that can potentially distort your assay results. x Avoid taking multiple luminometer readings on the same assay. The light output from ATP assays is relatively constant and at a maximum for the first 15-30 seconds after mixing, after which the output will decline. x When testing samples that yield low RLU values (i.e. RLU ATP LWLVUHFRPPHQGHGWRDFFRXQWIRUEDFNJURXQG noi se. Simply follow the procedure without adding any of the ATP-containing sample into the analysis and recor d this value as RLU bg . Typical RLU bg when using a PhotonMaster or Lumitester C-DUH If high RLU bg are consistently observed, repeat assays in an area out of direct sunlight or intense lighting. A single RLU bg may be used for multiple analyses much like a single UltraCheck 1 RLU (RLU ATP1 ).

Sampling Best Practices

Microorganisms are by their nature inherently more variable and heterogeneous as compared to physical or chemical constituents in a water sample. Therefore, one should not expect that an ATP test (let alone any other microbiological test) should be as repeatable or reproducible as compared to physical or chemical test methods. With that said, 2 nd Generation ATP tests are among the least variable microbiological tests, because they have been designed to be completely quantitative and objective.

When collecting samples, sterile bottles are not necessary because the analyses are completed before any microbial growth can occur. Also, contamination levels in bio-fouling application samples are usually much higher than would be present in a clean sample bottle. Overall, the greatest source of variability when performing ANY type of microbiological test is the sample itself.

Guidelines for Collecting Samples

x Prior to sampling water, remove any screens, aerators, swivel sprayers, etc. It is recommended to periodically remove and clean such devices in order to minimize the harboring of bacteria within the tap.

x Make sure to test samples within 2 hours of collection for the greatest accuracy. If samples cannot be tested within 2 hours of collection, store refrigerated (2 to 8 C) and test within 24 hours of collection.

x Keep the sample cool until it can be analyzed. The urgency is less for samples taken from low-nutrient environments. The urgency is higher in samples treated with biocide in which the timing of biocide action

www.luminultra.com © LuminUltra 2017 Page 13 of 20 needs to be defined. Once samples are collected, they are taken out of their natural environment so they should be analyzed as soon as possible.

x When shipping samples to be analyzed at a different location, it is often useful to collect multiple samples to compare on a relative basis. For example, send additional samples that have been treated with biocide to compare to an untreated sample.

x When dealing with systems dosed with biocides, sample location and sample collection timing are extremely important to assess biocide effectiveness. Factors to keep in mind are:

o Time required for the biocide to mix throughout the system and the fluid retention time

o Timing of associated plant operations

o Timing of peak challenges to the treatment program

o Biocide strength/kill rate

o The extent of system fouling prior to treatment (contamination recovery time is much shorter in a fouled system).

x Similarly, location issues include:

o The distance of sample source from the point of biocide application

o The proximity to most vulnerable and important system components

o The proximity to suspected contamination sources

o The proximity to heat and aeration sources

x The amount of time to let the water run depends on what part of the distribution system that is of interest.

o Within the faucet: Collect sample immediately

o Building Water Lines: Allow to run for 15 seconds

o Water Main: Allow to run for 5 minutes

x When handling the sample bottle, open the container without touching the inside of the cap or the mouth of the bottle. Fill the bottle to 2/3 full to allow sufficient headspace, provided that enough sample has been collected to complete the analysis.

x Mi x samples thoroughly before subsamples for ATP analyses are removed. However, vigorous mixing is not required because dispersion of microbial clumps and flocs is not necessary. In fact, mixing that is too vigorous may be stressful to some species, especially anaerobes located within the particulate components of the sample.

x Grab samples are always preferred for microbiological testing rather than composite samples. Composite samples are rarely representative of their environment because they represent isolated, new systems that are separate from their actual environment.

Visit the LuminUltra Academy section at www.luminultra.com/luminultra-academy/ for instructions on how to best set up your routine test program for your target application.

Validation

Although our products are industry-tested and proven in all foreseeable situations, some customers still desire to perform their own performance validations prior to initiating a routine monitoring program with LuminUltra’s products. Visit the Resources section of the website at https://www.luminultra.com/resource/2nd-generation-atp-product- validation-guidelines/ on how to best go about validating our products for your application.

FAQ & Troubleshooting x You have questions, we have answers! Visit the Support section of www.luminultra.com and consult the ‘Frequently Asked Questions (FAQ)’ page for the most up-to-date official set of questions and answers regarding our products. You may also feel free to contact us at any time via our website, email or phone. x LuminUltra’s test kits are the most robust and quantitative ATP test kits available. If you suspect you are enc ountering interferences in your application of these kits, first check to ensure you are using the correct kit typ e for your sample. If you are unsure or feel you have unique testing requirements, contact LuminUltra’s support staff for assistance. x Fluid samples with high solids content or complex nature (e.g. paints, coatings, slurries, adhesives) may require pre-dilution prior to analysis to ensure reliable quantification of ATP. For best results, use our QG21S test kit, or contact LuminUltra for recommendations on pre-dilution or use one of our pre-dilution kits for maximum compatibility with the analysis procedure. x If dropper bottles become plugged or you encounter difficulty dispensing drops, remove and discard the dropper tip and use a pipettor to measure and dispense the reagent.

How Does This Technology Work?

Visit the Technology section of www.luminultra.com for the most up-to-date and detailed information on our products, how they work, how they compare to the competition, and how they can benefit you!

Why Use the UltraCheck 1 ATP Standard?

Many suppliers of ATP products, especially those that supply ATP “pens”, claim that if they are used within the expiry date, it is not necessary to routinely calibrate their products. Furthermore, they advocate recording only instrument display (i.e. relative light units, or RLU) rather than converting results into ATP concentration.

When quantitative analyses are required, this policy is valid only if the following assumptions are true:

x The activity of the luciferase product is constant for each manufactured batch.

x The activity of the luciferase enzyme does not change with product storage.

x The storage properties of different batches of reagent will not change.

x Storage conditions will always be constant, including transit via courier.

x The refrigerator used to store the luciferase products will provide constant and uniform cooling.

x That if reagents are carried to another destination (for example, by a service representative), that stora ge conditions will always be ideal.

The performance of the luminometer is constant with age and in changing environmental conditions.

In truth, it is impossible for these conditions to be completely met.

Another problem with simply using the instrument display values is that after acquiring an extensive body of data, the user becomes more dependent on a single supplier that provides instruments and reagents that perform similarly to the products that were used to generate the past data. In other words, it will be undesirable for the user to switch to alternative, possibly superior and/or more cost-effective products from other suppliers.

Part of the reason that many companies avoid selling ATP standards is that most ATP solutions are unstable. Typically, ATP solutions are prepared from crystalline ATP to form a concentrated stock which is then stored frozen. Thus, use of a conventional standard is very cumbersome. However, LuminUltra Technologies has developed ATP standards (the UltraCheck TM product line) that are stable at use concentrations (nanograms of ATP per mL) for months, even at room temperature. This ensures that results can be compared with confidence from one time period to another and for one application to another.

Qualification Data

The main challenges to creating a reliable ATP standard formulation are to ensure that it remains stable for an extended period of time and also at different concentrations. If these requirements are not met, the use of an ATP standard becomes a moot point since the results of standard RLUs could not be fully trusted. The following data provides validation that UltraCheck ATP standards meet these requirements:

www.luminultra.com © LuminUltra 2017 Page 16 of 20 Long-Term Stability: In order to validate 20000 the long-term stability of UltraCheck 1, 18000 several batches were assayed in duplicate 16000 14000 using the same Luminase batch and 12000 compared to the output of a standard assay 10000 using a new batch. Results are shown in 8000

Average RLU Average 6000 Figure 3. 4000 2000 UltraCheck 1 maintains stability extremely 0 well over time at room temperature. Time UC1-10 UC1-11a4 UC1-13c UC1-16a UC1-17a Age = 4 Age = 3 Age = 2 Age = 1 year Age = does not appear to be a significant factor at years years years Freshly all since the oldest batch agreed most Made closely with the fresh one. Figure 3: Comparison of new and old UC1 Standard results.

Linearity: Theoretically, plotting a 6 calibration curve to convert RLU results y = 0.95x + 4.21 into actual ATP concentrations should not 5 R² = 0.9992 be necessary since assay light production is linear over many orders of magnitude. 4 However, reagent activity and instrument 3 quality can both create issues with this relationship so linearity must be confirmed. 2 Figure 4 shows the results of standard Log (RLU (RLU Log Produced) assays using a set LuminUltra’s UltraCheck 1 standards. RLU can be used to make this conversion. Clearly, the results showed 0 -4 -3 -2 -1 0 1 2 excellent linearity. This confirms good operation of the luminometer as well as Log (UltraCheck Concentration in ng/mL) stable ATP concentrations in UltraCheck formulations. Figure 4: Plot of UltraCheck standard RLUs

Quantitative ATP Results: Perhaps the biggest benefit of an ATP standard is its capability to convert RLU measurements into ATP concentrations. In general, the calculation used to convert RLU values into ATP concentrations is as follows:

[ ] = 1 ݎݐ݋ܿܽܨ ݑݐ݅݋݈݊݅ܦ ݌݈݁݉ܽݏ ܷܮܴ ܣܶܲ ൬ ൰ ൬ ൰ ݌݈݁ ܸ݋݈ݑ݉݁݉ܽܵ ݄݇ܿ݁ ܥܽݎݐ݈ܷ ܷܮܴ This allows the user to account for all other variables (e.g. enzyme degradation, temperature, instrument make and model, etc.). As such, testing a sample using different luciferase enzyme reagents (but using the same test protocol) will produce the same ATP concentrations once the standard is taken into account. Figures 5 and 6 illustrate this.

160,000 UltraCheck 1 RLU 151,548 2,000 1,762 1,797 1,685 1,743 1,704 140,000 129,122 1,586 120,000 1,500 103,997

100,000 93,438 RLU 80,000 1,000 pg cATP/mL pg 60,000 40,000

21,753 500 17,253

20,000 8,468 7,380 3,299 2,906 1,116 940 0 0

Figure 5: Sample RLU results from QGO-M tests on Figure 6: Comparison of calculated ATP metalworking fluids in addition to UltraCheck 1 RLU concentrations from QGO-M analyses using values using three Luminase formulations. UltraCheck 1 ATP standard (CV = 4%).

Overall, conversion of RLU values to ATP concentrations is a necessity to ensure that ATP test results can be trusted. Otherwise, the analysis is essentially an un-calibrated test that is subject to a plethora of error sources. LuminUltra’s UltraCheck ATP standards provide the ability to correct for all external factors to leave only the ATP content of the sample and the dilution factor of the test protocol to determine the RLU value produced.

Glossary of Terms

The following terms and acronyms are found throughout our product documentation. For the most up-to-date glossary of terms, please visit the Support section of www.luminultra.com . x ATP: Adenosine Triphosphate x Active Biomass Ratio (ABR): The ratio of AVSS to TSS; in other words, the proportion of total suspended matter that is living biomass for a given sample. x Active Volatile Suspended Solids (AVSS): The concentration of living, suspended matter in the sample. Calculated by converting the cATP from ng ATP/mL to mg Biomass/L using third-party correlations. x Assay (or ATP Assay): Measurement of the light produced when an ATP-containing mixture is mixed with Luminase and placed in a luminometer. x Biomass Stress Index (BSI): The ratio of Extra-cellular to Total (Intra-cellular + Extra-cellular) ATP. Put simply, it is the proportion of ATP in the sample that is outside of living cells.· A higher BSI is indicative of greater stress. x Cellular ATP (cATP): LuminUltra's terminology for Intra-cellular ATP measurement or quantification. x Colony Forming Units (CFU): A conventional unit of measure for culture-based microbiological test methods. Each colony that is counted after a specific time period is 1 CFU. x Diluted Extract: The UltraLyse-treated portion of sample that is diluted prior to the assay step. x Diluted Sample: A portion of sample diluted in a stabilizing buffer for dATP measurement. x Dilution Factor (DF): The factor by which the user must multiply the standardized assay RLU to compensate for the dilution of the original sample (to remove inhibition). x Dissolved ATP (dATP): LuminUltra's terminology for Extra-cellular ATP measurement or quantification.

www.luminultra.com © LuminUltra 2017 Page 18 of 20 x DSA: Deposit and Surface Analysis x Extra-cellular ATP : The portion of sample ATP not associated with (i.e. contained outside) microorganisms. x Extract/ATP Extract/Sample Extract: The mixture produced when a sample is treated with an ATP extraction agent (i.e. UltraLyse). Diluted Sample: A portion of sample diluted in a stabilizing buffer for dATP measurement. x fg/mL: Femtogram per milliliter. This is the standard unit for ATP concentrations in single cells, and is equivalent to "parts per trillion" (ppt). x Floc-Bulking ATP (fbATP): A measurement of ATP associated with bulking floc, achieved by pre-screening the sample and measuring the Total ATP retained on that screen. x Intra-cellular ATP: The portion of sample ATP associated with (i.e. contained within) microorganisms. x Luminometer: Instrument responsible for measuring the RLU emitted during an assay. x Microbial Equivalents (ME): A conversion of 1 fg ATP into 1 E. Coli sized cell. Used for putting ATP results on the same scale of measurement as CFU. x ng/mL: Nanogram per milliliter. This is the standard unit for ATP concentrations in wastewater treatment applications, and is equivalent to "parts per million" (ppm). x pg/mL: Picogram per milliliter. This is the standard unit for ATP concentrations, and is equivalent to "parts per billion" (ppb). x QG21I: Quench Gone 21 Industrial x QG21S: Quench Gone 21 Specialty x QG21W: Quench Gone 21 Wastewater x QGA: Quench Gone Aqueous x QGO-M: Quench Gone Organic Modified x RLU: Relative Light Unit. This is the instrument display (i.e. the amount of light output) on a luminometer after an assay has been completed. x Sample: The sample from the process to be tested for ATP concentration. x Specific Floc-Bulking ATP (s-fbATP): The ratio of fbATP to tATP; in other words, the specific proportion of ATP associated with bulking floc relative to the total amount. x Total ATP (tATP): The total amount of ATP in the sample. Intra-cellular + Extra-cellular. x Total Suspended Solids (TSS): The total concentration of suspended matter in a given sample. Includes all particles, both inorganic and organic, a subset of which is living biomass. x Volatile Suspended Solids (VSS): The concentration of organic suspended matter in the sample. Includes only organic particles, a subset of which is living biomass.

Shipping x All prices are FOB Fredericton, NB, Canada. x Transportation charges and fees are not included in the quotes and are the responsibility of the purchaser. x Duties, taxes and import fees are billed separately from transportation charges by the import customs broker and are the responsibility of the importer/purchaser. x For orders of 5 or fewer kits and loose items, the order will normally be shipped within a maximum of 3 business days. x For orders of 6 to 10 kits, loose items that exceed our normal stock or non–stock items, the order will generally be shipped within 5 business days depending on availability of the products. x For Wholesale orders of 10 kits or more the order will normally be shipped within a minimum of 10 Business days. x LuminUltra is not responsible for any late deliveries caused by customs delays and freight carrier errors.

Ordering x Quotations are valid for 30 days from the date of issue. x Orders can be placed by phone, e–mail or fax. An order confirmation will be sent upon receipt. When submitting an order please include your preferred carrier and mode of transport. International Shipments will require the EIN, Tax ID or VAT number of the purchaser.

Payment Terms x Unless otherwise specified, all invoices (invoicing subject to credit approval) are Net 30 days. Overdue invoices are subject to a 2% per month (26.82% per annum) charge. x Visa, MasterCard and Amex payments are accepted.

x All Prices are subject to change without notice.

Return Policy x Requests for product returns must be issued to LuminUltra within 30 days of receipt to be considered, otherwise all sales are final. x Returns are subject to a 25% restocking fee plus shipping and handling charges.

Contact

To inquire about services, request a quotation, or to place an order, contact us today! x To place an order: [email protected] x For all other inquiries: [email protected] x Tel: +1 (506) 459–8777 x Fax:+1 (506) 453–9860