Physicians Office Labs
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Maintenance of Bacterial Strains
Maintenance of Bacterial Strains Short-term growth and maintenance The simplest method for obtaining strains is to order them from a microbiological supply company. Presque Isle, Ward, and Carolina Biological (see “Resources” page of the Microbes Web site: www.umsl.edu/~microbes) are economical sources of strains that are commonly used in the classroom. Another possible source is a local college or university that teaches a microbiology lab course. If they have strains already prepared for their class, they will probably be willing to provide you with a culture. This is probably not a reasonable ongoing source of strains, but can be useful in an emergency. For short-term maintenance and use, it is best to streak bacteria on some type of rich agar petri plate, such as nutrient agar. Label and date the plate on the bottom of the plate. Streak from the source culture using good sterile technique. The quadrant streak method (see the article on quadrant streaking) is good for obtaining well-isolated colonies. Incubate the plate only until good colonies have formed. Do not leave the culture in the incubator beyond that time, or the cells will die on the plate. Store the plate in the refrigerator. It will keep for about a month. For class use, it is best to streak a fresh plate 2-3 days before the strain will be needed for class. Use the fresh plate as the source of cultures for the class. For broth cultures for a class, it is best to grow a larger volume culture in a flask (if possible, with shaking) overnight and then dispense aliquots of the master culture into sterile test tubes using a sterile pipette. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Laboratory Supplies and Equipment
Laboratory Supplies and Equipment Beakers: 9 - 12 • Beakers with Handles • Printed Square Ratio Beakers • Griffin Style Molded Beakers • Tapered PP, PMP & PTFE Beakers • Heatable PTFE Beakers Bottles: 17 - 32 • Plastic Laboratory Bottles • Rectangular & Square Bottles Heatable PTFE Beakers Page 12 • Tamper Evident Plastic Bottles • Concertina Collapsible Bottle • Plastic Dispensing Bottles NEW Straight-Side Containers • Plastic Wash Bottles PETE with White PP Closures • PTFE Bottle Pourers Page 39 Containers: 38 - 42 • Screw Cap Plastic Jars & Containers • Snap Cap Plastic Jars & Containers • Hinged Lid Plastic Containers • Dispensing Plastic Containers • Graduated Plastic Containers • Disposable Plastic Containers Cylinders: 45 - 48 • Clear Plastic Cylinder, PMP • Translucent Plastic Cylinder, PP • Short Form Plastic Cylinder, PP • Four Liter Plastic Cylinder, PP NEW Polycarbonate Graduated Bottles with PP Closures Page 21 • Certified Plastic Cylinder, PMP • Hydrometer Jar, PP • Conical Shape Plastic Cylinder, PP Disposal Boxes: 54 - 55 • Bio-bin Waste Disposal Containers • Glass Disposal Boxes • Burn-upTM Bins • Plastic Recycling Boxes • Non-Hazardous Disposal Boxes Printed Cylinders Page 47 Drying Racks: 55 - 56 • Kartell Plastic Drying Rack, High Impact PS • Dynalon Mega-Peg Plastic Drying Rack • Azlon Epoxy Coated Drying Rack • Plastic Draining Baskets • Custom Size Drying Racks Available Burn-upTM Bins Page 54 Dynalon® Labware Table of Contents and Introduction ® Dynalon Labware, a leading wholesaler of plastic lab supplies throughout -
ED149972.Pdf
-74 ..t.rcrVtr.,,,.. r . ", 0 ' 4^Az7 .. ' . DOCUMNIT ant= 2D 149 972 SE 023- 413 AUTHOR . We'sterhold, Arnold F.., Ed.; 'Bennett; Ernest C., . Ed. , _ TITLE : Ahanual of Simplified Laboratory Methods for . Operators of 'Wastewater Treatment Facilities.. INSTITUTION' Illinois State Epvircidental.Protection Agencyc , .. - 'Springfield.. PUB DATE Apr 74 NOTE 94p.; Pages 1-1 through 1-12 (General Introduction) removed due to copyright restrictions; Section 8 'missing; Contains occasional light type; Best Copy / Available EDRS PRICE .M7-$0.83 HC-$4.67 Plus Postage. DESCRIPTORS, Chemistry; Environmental Education; *Environmental Technicians; Independent Study; *Instructional Materials; Job Skills; *Laboratory Tchniques; *Pollution; *Post Seccndary Education; Public Health; *Water Pollution Control IDENTIFIERS cf*Waste Water Treatment ABSTRACT . ./ This manual is designed to provide the seall wastewater treatment plant operator, as well as the new or inexperienced operator, with simplified methods for laboratory analysis of'water"and wastewater. It is emphasized that this manual is not a replacement for standard methods but a guide for plantswith insufficient equipment to pefform analyses ifi..accordance with ,standard methods. Eacj, of the sections is designed to becomplete I within itself. The tests and measurements 'presented include: acids, biochethical oxygen demand (BOD);40674plved oxygen, residues, sludge, a'nd suspended solids. (CS) el. -V or 4. ***********44*************************************i*******i*******4S*** I4 * Reproductions -
Qualitube™ Kit for Microcystin
™ QualiTube Kit For Microcystin Highlights: Catalog Number ET 022 Semi-quantitative field screening Intended Use of Microcystin toxin in surface water The EnviroLogix QualiTube Kit for Microcystin is designed for semi- Detects from 0.5 to 3 ppb quantitative field screening of Microcystin toxin in surface water samples. The kit is supplied with calibrators at 0.5 and 3 ppb. The assay range can easily be extended. How the Test Works Contents of Kit: The QualiTube Kit for Microcystin is a competitive Enzyme-Linked ImmunoSorbent Assay (ELISA). In the test, Microcystin toxin in the sample 36 antibody-coated test tubes competes with enzyme (horseradish peroxidase)-labeled Microcystin for a 1 vial of 0.5 ppb Microcystin LR limited number of antibody binding sites on the inside surface of the test Calibrator tubes. 1 vial of 3 ppb Microcystin LR Calibrator After a simple wash step, the outcome of the competition is visualized with 1 dropper bottle of Assay Diluent a color development step. As with all competitive immunoassays, sample 1 dropper bottle of Microcystin- concentration is inversely proportional to color development. enzyme Conjugate 1 dropper bottle of Substrate Darker color = Lower concentration 1 bottle of Stop Solution Lighter color = Higher concentration 36 sample pipettes Limit of Detection The Limit of Detection (LOD) of the EnviroLogix Microcystin Tube Kit is Optional Accessory Item: 0.3 ppb. The LOD was determined by interpolation at 81.5% B0* from a ACC 062 – 1 vial of 1.5 ppb standard curve. 81.5% B0 was determined to be 2 standard deviations from Microcystin LR Calibrator the mean of a population of negative water samples. -
Laboratory Equipment Used in Filtration
KNOW YOUR LAB EQUIPMENTS Test tube A test tube, also known as a sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom. Beakers Beakers are used as containers. They are available in a variety of sizes. Although they often possess volume markings, these are only rough estimates of the liquid volume. The markings are not necessarily accurate. Erlenmeyer flask Erlenmeyer flasks are often used as reaction vessels, particularly in titrations. As with beakers, the volume markings should not be considered accurate. Volumetric flask Volumetric flasks are used to measure and store solutions with a high degree of accuracy. These flasks generally possess a marking near the top that indicates the level at which the volume of the liquid is equal to the volume written on the outside of the flask. These devices are often used when solutions containing dissolved solids of known concentration are needed. Graduated cylinder Graduated cylinders are used to transfer liquids with a moderate degree of accuracy. Pipette Pipettes are used for transferring liquids with a fixed volume and quantity of liquid must be known to a high degree of accuracy. Graduated pipette These Pipettes are calibrated in the factory to release the desired quantity of liquid. Disposable pipette Disposable transfer. These Pipettes are made of plastic and are useful for transferring liquids dropwise. Burette Burettes are devices used typically in analytical, quantitative chemistry applications for measuring liquid solution. Differing from a pipette since the sample quantity delivered is changeable, graduated Burettes are used heavily in titration experiments. -
10. Resource Requirement for Medical Laboratory Technology
10. RESOURCE REQUIREMENT FOR MEDICAL LABORATORY TECHNOLOGY 10.1 PHYSICAL RESOURCES 10.1.1. SPACE REQUIREMENT The total space for lecture halls, laboratories may be worked out as per latest AICTE norms 10.1.2 LIST OF EQUIPMENT Note: All the glass wares to be of Borosil make The chemicals may be purchased of reputed make. Sr. Item Quantity No. LIST OF CHEMICALS FOR CHEMISTRY LABORATORY 1. Ferrous ammonium sulphate (Analytical reagent) 3 x 500 grams 2. Oxalic Acid (A.R) 2 x 500 grams 3. Potassium Permanganate (BDH) 3 x 500 grams 4. Sodium Hydroxide (BDH) 2 x 500 grams 5. Sulphuric Acid (AR) 5 litre 6. Hydrochloric Acid (AR) 5 litre 7. Sodium carbonate (A.R) 2 x 500 grams 8. Phenolphthalen (BDH/Merck) 10 grams 9. Methyl orange (SDH) 10 grams 10. Ethyl Alcohol 4 x 500 ml 11. Methyl Alcohol 500 ml 12. Nitric acid 5 litre 13. Glacial Acetic Acid (CP) 1 litre 14. Ammonium sulphate (BDH) 250 grams 15. Copper sulphate (BDH) 500 grams 16. Glucose (BDH) 500 grams 17. Sucrose (BDH) 500 grams 18. Acetone (BDH) 500 grams 19 Potassium Hydroxide (BDH) 2 x 500 grams 20. Iodine 1 x 500 grams 21. Calcium Chloride (BDH) 500 grams 93 Sr. Item Quantity No. 22 NaCl 500 gms 23. Ferric chloride (BDH) 500 grams 24. Starch pH 7.0 500 grams 25. Fructose 250 grams. 26. Gelatin 500 grams. 27. Benzaldehyde 500 ml 28. Ether 500 ml 29. Carbon Tetrachloride 500 ml 30. Ferrous Bicarbonate 500 grams 31. Sodium Bicarbonate 500 grams 32. Albumin 500 grams 33. -
Practical Laboratory Skills for Molecular Biologists.Pdf 3.0 MB
Practical laboratory skills for molecular biologists skills best practice training training best practice skills Practical laboratory skills for molecular biologists First Edition 2016 Co-authors Elena Sanchez Timothy Wilkes Contributors Nicholas Redshaw Acknowledgements With special thanks to Alison Woolford and Vicki Barwick for their help in the production of this guide. Production of this Guide was funded by the UK National Measurement System. First edition 2016 ISBN: 978-0-948926-28-0 This publication should be cited as: E Sanchez, T Wilkes, Practical laboratory skills for Copyright © 2016 LGC molecular biologists, LGC 2016. ISBN: 978-0-948926-28-0 Practical laboratory skills for molecular biologists OBJECTIVES OF THIS GUIDE 3 1 WORKING IN THE LABORATORY 4 1.1 Health and Safety issues 4 1.2 Laboratory environment 10 1.3 Equipment 14 1.4 Method selection 15 2 MEASURING VOLUME 16 2.1 Types of equipment available 16 2.2 Markings on equipment used for volumetric measurements 22 2.3 Selecting a suitable piece of equipment 24 2.4 Cleaning and maintenance of volumetric equipment 26 2.5 Checking the accuracy of volumetric equipment 27 2.6 Checklists for making measurements of volume 28 3 MEASURING MASS 33 3.1 Mass versus weight 33 3.2 Types of balance available 33 3.3 Selecting a suitable balance 34 4 QUANTIFICATION OF NUCLEIC ACID MATERIAL 41 4.1 Measurement of nucleic acid concentration using UV spectroscopy 41 4.2 Fluorescent based systems. 43 4.3 Gel electrophoresis 45 5 MEASURING PH 47 5.1 What is pH? 47 5.2 Equipment for measuring -
BLAUBRAND® Volumetric Instruments and Density Bottles
BLAUBRAND® Volumetric Instruments and Density Bottles Testing Instructions (SOP) March 2015 1. Introduction The standard DIN EN ISO 4787 describes both the design and the testing of the volumetric instruments of glass. The following Testing Instructions describe how to apply the ISO standard in practice. We recommend a testing every 1-3 years. The interval depends on the using of aggressive media and the cleaning procedure. These Instructions may also be used as a basis for the supervision of testing devices to DIN EN ISO 9001, DIN EN ISO 10012 and DIN EN ISO/IEC 17025. The test of the Density Bottles was effected on the basis of DIN EN ISO 4787. Meniscus adjustment with BLAUBRAND® Volumetric Instruments Meniscus adjustment Meniscus adjustment with ring mark with Schellbach stripe Read at the lowest point of the meniscus. Read at the point where the two arrows touch. Meniscus adjustment reflexion of liquid surface meniscus ring mark dark paper (p.e. black, blue) 2 2. Preparation for testing 2. Clear identification of the volumetric instrument to be tested Batch number, individual serial number, trademark, nominal volume and tolerances are directly printed ⇒ The test starts with a clear identification of the on every BLAUBRAND volumetric instruments. volumetric instrument in the test record. 2.1 Copy Test Record (see page 13) 2.2 Serial number/Identification number ⇒ Enter into Test Record All BLAUBRAND® volumetric instruments always carry a batch number, e.g., 13.04, or an individual serial number in the case of individual certificates, e.g., 13.040371 (year of production 2013, Batch No. -
List of Equipment
Mfg & Exporters: All types of Turnkey Projects, , Beverages, Mineral Water plant & OEM Spares List Of Equipment SR.NO Equipment Qty 1. Autoclave 1 Vertical Complete Heavy gauze with pressure gauze, Safety Valve, Stream Release Valve Size 30 X 30 cm 2. Digital Hot Air Ageing oven 1 Size 12” x 12” x 12” made of MS powder coated. Make- HB 3. Digital pH System : 1 Make- HB 4. Digital Incubator Bacteriological 2 12” X 12” X 12” made of MS powder coated. Make- HB 5. Water bath double walled 1 inner chamber of SS outer M.S powder coated with 6 hole with thermostatic control Make- HB 6. INNOCULATION CHAMBER, M.S POWDER COATED WITH 1 UV & 1 FLOROCENT LIGHT INBUILT 1 Make- HB 7. Automatic Hot Plate with 8 inch dia with Thermostat 1 Make-HB 8. Digital Spectrophotometer 1 Make- HB 9. Digital Turbidity meter 1 Make- HB 10. Digital Colony Counter 1 Make- HB 11. S.S. Filter holder 47 mm 1 (complete S.S) 12. Microscope student 1 Make-Labron 13. Magnetic Stirrer with Hot Plate with 1 lit capacity Top made of SS with Teflon Bed 1 Make- HB 14. Centrifuge ( 4 holders) 1 Make- HB 15. Single pan Balance ( pocket type) max: 0-500 gm, L.C: 0.1 gm 1 16. High pressure vacuum Pump 1 17. BOD Incubators inner complete S.S outer MS powder coated.( heavy) 2 Make – HB 18. Heating Mental 1 lit capacity 1 Make- HB 19. S.S distillation Unit 1 Address: 140, Tribhuvan Estate opp Road No 11 Kathwada GIDC Contact: +91 9726943972 Email: [email protected] Mfg & Exporters: All types of Turnkey Projects, , Beverages, Mineral Water plant & OEM Spares List Of Chemicals Sr. -
J-Sil Price List
An ISO 9001 : 2008 Certified Company TM J-SIL LABORATORY GLASSWARE Made from Borosilicate 3.3 Glass PRICE LIST 1st April, 2015 Manufactured by : J-SIL SCIENTIFIC INDUSTRIES MFRS. OF : SCIENTIFIC & LABORATORY GLASS APPARATUS 48-B, INDUSTR IAL ESTATE, NUNHAI, AGRA-282006 (INDIA) Phone : 2281967, 2281887 l Fax : 0562-2280186 E-mail : jsil @sancharnet.in, [email protected] Web : www.j-sil.com LABORATORY GLASS APPARATUS J-SIL 51. Chromatography Graduated, Screw Vials 9 mm wide Opening Type Cap Pkt Price/ Pkt A Clear Glass 2ml 100 PC 650.00 B Amber Glass 2ml 100 PC 700.00 56. Screw Cap with Septa for 9 mm Screw Vials (Closure) Cap Septa Pkt Price/ Pkt A Blue Non Slit 100 PC 750.00 B Blue Pre-Slit 100 PC 775.00 61. Clear Glass Screw Vials 9mm with Screw Blue Cap & PTFE-Silicon Septa (Combo) Cap Septa Pkt Price/ Pkt A 2 ml Non Slit 100 PC 1350.00 B 2 ml Pre-Slit 100 PC 1400.00 66. Amber Clear Glass Screw Vials 9mm with Screw Blue Cap & PTFE-Silicon Septa (Combo) Cap Septa Pkt Price/ Pkt A 2 ml Non Slit 100 PC 1400.00 B 2 ml Pre-Slit 100 PC 1450.00 LABORATORY GLASS APPARATUS J-SIL 71. Nylon Syringe Filter (Non Sterile) Packed in Transparent Plastic Jar DIA MICRON Pkt Price/ Pkt A 13 mm 0.22µm 100 1800.00 B 13 mm 0.45µm 100 1800.00 C 25 mm 0.22µm 100 1850.00 D 25 mm 0.45µm 100 1850.00 76. PVDF Syringe Filter (Non Sterile) Packed in Transparent Plastic Jar DIA MICRON Pkt Price/ Pkt A 13 mm 0.22µm 100 2800.00 B 13 mm 0.45µm 100 2800.00 C 25 mm 0.22µm 100 2850.00 D 25 mm 0.45µm 100 2850.00 81. -
A Passive Membrane Photobioreactor for the Isolated Cultivation of Algal
University of South Florida Scholar Commons Graduate Theses and Dissertations Graduate School 10-31-2014 A Passive Membrane Photobioreactor for the Isolated Cultivation of Algal Resource Utilizing Selectivity (ICARUS), with Wastewater as a Feedstock Ivy Lea Cormier Drexler University of South Florida, [email protected] Follow this and additional works at: https://scholarcommons.usf.edu/etd Part of the Civil and Environmental Engineering Commons Scholar Commons Citation Drexler, Ivy Lea Cormier, "A Passive Membrane Photobioreactor for the Isolated Cultivation of Algal Resource Utilizing Selectivity (ICARUS), with Wastewater as a Feedstock" (2014). Graduate Theses and Dissertations. https://scholarcommons.usf.edu/etd/5414 This Dissertation is brought to you for free and open access by the Graduate School at Scholar Commons. It has been accepted for inclusion in Graduate Theses and Dissertations by an authorized administrator of Scholar Commons. For more information, please contact [email protected]. A Passive Membrane Photobioreactor for the Isolated Cultivation of Algal Resource Utilizing Selectivity (ICARUS), with Wastewater as a Feedstock by Ivy L.C. Drexler A dissertation submitted in partial fulfillment of the requirements for the degree of Doctor of Philosophy in Engineering Science Department of Civil and Environmental Engineering College of Engineering University of South Florida Co-Major Professor: Daniel H. Yeh, Ph.D. Co-Major Professor: Norma Alcantar, Ph.D. Sarina Ergas, Ph.D. Valerie Harwood, Ph.D. Piet Lens, Ph.D. Date of Approval: October 31, 2014 Keywords: biofuel, dialysis, nutrients, phycoprospecting, phycoremediation Copyright © 2014, Ivy L.C. Drexler Dedication To Mike, my husband, The smartest person I know. And our little babe.