Experiment 1 Check-In, Safety, Identification of Lab Equipment, and an Introduction to Graphing
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Lab Equipment Erlenmeyer Flask
Lab Equipment Erlenmeyer Flask • Erlenmeyer flasks hold solids or liquids that may release gases during a reaction or that are likely to splatter if stirred or heated. Beaker • Beakers hold solids or liquids that will not release gases when reacted or are unlikely to splatter if stirred or heated. Graduated Cylinder • A graduated cylinder is used to measure volumes of liquids. Test Tubes • Test tubes are used to hold small amounts of liquid and to conduct small chemical reactions in. Test Tube Clamp • A test tube holder is useful for holding a test tube which is too hot to handle. Test tube and beaker Brushes • Test tube brushes are used to clean test tubes and graduated cylinders. • Forcing a large brush into a small test tube will often break the tube. Test Tube Racks • Test tube racks are for holding and organizing test tubes on the laboratory counter. Plastic racks may melt in contact with very hot test tubes. Spot Plates • Spot plates are used when we want to perform many small scale reactions at one time. We will use these many times during the year. Watch Glass • A watch glass is used to hold a small amount of solid, such as the product of a reaction. Glass Stir Rod • A glass rod is used to manually stir solutions. It can also be used to transfer a single drop of a solution. Funnel • A funnel is used to aid in the transfer of liquid from one vessel to another. • A funnel is used to separate a solid from a liquid Wash Bottle • A wash bottle has a spout that delivers a wash solution to a specific area. -
Laboratory Glassware N Edition No
Laboratory Glassware n Edition No. 2 n Index Introduction 3 Ground joint glassware 13 Volumetric glassware 53 General laboratory glassware 65 Alphabetical index 76 Índice alfabético 77 Index Reference index 78 [email protected] Scharlau has been in the scientific glassware business for over 15 years Until now Scharlab S.L. had limited its sales to the Spanish market. However, now, coinciding with the inauguration of the new workshop next to our warehouse in Sentmenat, we are ready to export our scientific glassware to other countries. Standard and made to order Products for which there is regular demand are produced in larger Scharlau glassware quantities and then stocked for almost immediate supply. Other products are either manufactured directly from glass tubing or are constructed from a number of semi-finished products. Quality Even today, scientific glassblowing remains a highly skilled hand craft and the quality of glassware depends on the skill of each blower. Careful selection of the raw glass ensures that our final products are free from imperfections such as air lines, scratches and stones. You will be able to judge for yourself the workmanship of our glassware products. Safety All our glassware is annealed and made stress free to avoid breakage. Fax: +34 93 715 67 25 Scharlab The Lab Sourcing Group 3 www.scharlab.com Glassware Scharlau glassware is made from borosilicate glass that meets the specifications of the following standards: BS ISO 3585, DIN 12217 Type 3.3 Borosilicate glass ASTM E-438 Type 1 Class A Borosilicate glass US Pharmacopoeia Type 1 Borosilicate glass European Pharmacopoeia Type 1 Glass The typical chemical composition of our borosilicate glass is as follows: O Si 2 81% B2O3 13% Na2O 4% Al2O3 2% Glass is an inorganic substance that on cooling becomes rigid without crystallising and therefore it has no melting point as such. -
Q Path Adhesive Slide
In Vitro Diagnostic Medical Device For professional use only Q PATH ADHESIVE SLIDE According to standard ISO 18113-2 : 2009, Point 7 Requirements for instructions or use and consolidated Directive 98/79/EC PRODUCT NAME Cat. No Description Pack Size 10048001 Q path Adhesive Slide white Box of 72 slides 10048002 Q path Adhesive Slide blue Box of 72 slides 10048003 Q path Adhesive Slide green Box of 72 slides 10048004 Q path Adhesive Slide yellow Box of 72 slides 10048005 Q path Adhesive Slide pink Box of 72 slides INTENDED PURPOSE These microscope slides may be used for the routine diagnosis of suspensions of cells and samples of tissue. Given that the possibilities for their use are highly varied, these glass slides should only be handled by users who hold a professional qualification that complies with national legislation. These microscope slides are intended for single use only. Any re-use or inappropriate treatment of their surface could lead to inaccurate results, the deterioration of preparations, and erroneous diagnoses. WARNING AND PRECAUTIONS Before use, please read all this information carefully. Microscope slides and cover slips are intended for single use by qualified personnel. They must under no circumstances be used more than once, and must be disposed of after use or at the end of the archive period in an appropriate manner as potentially infectious waste. If you observe any damage to the packaging, or any signs of glass fragments, the slides and cover slips must not be used, due to the risk of injury from glass splinters. Recommendation: In order to reduce the risk of injury due to sharp edges as much as possible, we strongly recommend the use of our microscope slides with polished edges. -
Maintenance of Bacterial Strains
Maintenance of Bacterial Strains Short-term growth and maintenance The simplest method for obtaining strains is to order them from a microbiological supply company. Presque Isle, Ward, and Carolina Biological (see “Resources” page of the Microbes Web site: www.umsl.edu/~microbes) are economical sources of strains that are commonly used in the classroom. Another possible source is a local college or university that teaches a microbiology lab course. If they have strains already prepared for their class, they will probably be willing to provide you with a culture. This is probably not a reasonable ongoing source of strains, but can be useful in an emergency. For short-term maintenance and use, it is best to streak bacteria on some type of rich agar petri plate, such as nutrient agar. Label and date the plate on the bottom of the plate. Streak from the source culture using good sterile technique. The quadrant streak method (see the article on quadrant streaking) is good for obtaining well-isolated colonies. Incubate the plate only until good colonies have formed. Do not leave the culture in the incubator beyond that time, or the cells will die on the plate. Store the plate in the refrigerator. It will keep for about a month. For class use, it is best to streak a fresh plate 2-3 days before the strain will be needed for class. Use the fresh plate as the source of cultures for the class. For broth cultures for a class, it is best to grow a larger volume culture in a flask (if possible, with shaking) overnight and then dispense aliquots of the master culture into sterile test tubes using a sterile pipette. -
A Fiber Optic Ammonia Sensor Using a Universal Ph Indicator
Sensors 2014, 14, 4060-4073; doi:10.3390/s140304060 OPEN ACCESS sensors ISSN 1424-8220 www.mdpi.com/journal/sensors Article A Fiber Optic Ammonia Sensor Using a Universal pH Indicator Adolfo J. Rodríguez 1,*, Carlos R. Zamarreño 2, Ignacio R. Matías 2, Francisco. J. Arregui 2, Rene F. Domínguez Cruz 1 and Daniel. A. May-Arrioja 1 1 Fiber and Integrated Optics Laboratory, Electronics Engineering Department, UAM Reynosa Rodhe, Universidad Autónoma de Tamaulipas, Carr. Reynosa-San Fernando S/N, Reynosa, Tamaulipas 88779, Mexico; E-Mails: [email protected] (R.F.D.C.); [email protected] (D.A.M.-A.) 2 Departamento de Ingeniería Eléctrica y Electrónica, Universidad Pública de Navarra, Edif. Los Tejos, Campus Arrosadia, Pamplona España 31006, Spain; E-Mails: [email protected] (C.R.Z.); [email protected] (I.R.M.); [email protected] (F.J.A.) * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +52-899-9213300 (ext. 8315); Fax: +52-899-921-3300 (ext. 8050). Received: 27 December 2013; in revised form: 12 February 2014 / Accepted: 18 February 2014 / Published: 27 February 2014 Abstract: A universal pH indicator is used to fabricate a fiber optic ammonia sensor. The advantage of this pH indicator is that it exhibits sensitivity to ammonia over a broad wavelength range. This provides a differential response, with a valley around 500 nm and a peak around 650 nm, which allows us to perform ratiometric measurements. The ratiometric measurements provide not only an enhanced signal, but can also eliminate any external disturbance due to humidity or temperature fluctuations. -
ARC Laboratory Manual for Architectural Conservators
A LABORATORY MANUAL FOR ARCHITECTURAL CONSERVATORS Jeanne Marie Teutonico ICCROM Rome 1988 CONTENTS Foreword v Preface vii General Principles: Laboratory Science 1. Sampling 3 2. Measurement and Error: Precision, Accuracy, Statistics 7 3. Measurement: Mass (Use of the Balance) 11 4. Measurement: Length (Use of the Vernier Caliper and Micrometer) 16 5. Measurement: Volume 21 6. Measurement: Solutions 26 7. Measurement: pH 30 Porous Building Materials 8. Water Absorption by Total Immersion 35 9. Water Drop Absorption 41 10. Penetration of Water: Capillary Action 43 11. Porosity of Granular Beds 45 12. Porosity in Solids: Indirect Measurement by Water Absorption 50 13. Porosity in Solids: Hydrostatic Weighing 52 14. Movement of Salts 56 15. Salt Crystallization 57 16. Qualitative Analysis of Water-soluble Salts and This publication was printed with a generous Carbonates 58 contribution from the government of 17. Semiquantititive Analysis of Water-soluble Salts 68 Finland. Earthen Building Materials 18A. Particle Size Analysis: Part I Sieving Procedure 73 ISBN 92-9077-083-X Via di San Michele 13 00153 Rome RM, Italy Printed in Italy III Earthen Building Materials (continued) FOREWORD 18B. Particle Size Analysis: Part II Sedimentation Procedure: Hydrometer Method 83 19. Plastic Limit of Soils 96 After his appointment as director of ICCROM in 1977, Sir Bernard FEILDEN proposed to emphasize the interdisciplinary 20. Liquid Limit of Soils 102 character of conservation by introducing scientists to field work and architects to laboratories. Consequently it was decided to introduce a series of laboratory exercises Stone. Brick and Mortars designed for the needs of architectural conservators and conservation architects in the program of the International 21. -
Rebecca Has Samples of Different Types of Metal, and She Wants to Find the Density of Each
Rebecca has samples of different types of metal, and she wants to find the density of each. First, she measures the volume of each sample. Now she needs to measure the samples' masses. Which of the following tools should Rebecca use to measure the mass of each sample? A. measuring tape B. calipers C. graduated cylinder D. balance Question 2 Add Ken needs to measure out 50 mL of water. Which of the following is the best tool for Ken to use? A. balance B. ruler C. spring scale D. graduated cylinder Question 3 Add Emily sees the following tool in her science lab. What is this tool used to measure? A. an object's volume B. an object's density C. an object's mass D. an object's length Question 4 Add Tamora is heating a liquid to find the temperature at which the liquid boils. Which piece of laboratory equipment should Tamora use to measure the temperature of the liquid once the boiling has begun? A. timer B. balance C. microscope D. thermometer Question 5 Add Which of the following laboratory tools would be most appropriate for measuring the approximate volume of a liquid? A. thermometer B. beaker C. balance D. tweezers Question 6 Add Directions: Select the correct answer from each drop-down menu. Mass can be measured using a volume can be measured using a and length can be measured using a Question 7 Add Betty is studying vein patterns in leaves. Which of the following tools could help Betty examine the leaves' veins in greater detail? A. -
Laboratory Equipment Reference Sheet
Laboratory Equipment Stirring Rod: Reference Sheet: Iron Ring: Description: Glass rod. Uses: To stir combinations; To use in pouring liquids. Evaporating Dish: Description: Iron ring with a screw fastener; Several Sizes Uses: To fasten to the ring stand as a support for an apparatus Description: Porcelain dish. Buret Clamp/Test Tube Clamp: Uses: As a container for small amounts of liquids being evaporated. Glass Plate: Description: Metal clamp with a screw fastener, swivel and lock nut, adjusting screw, and a curved clamp. Uses: To hold an apparatus; May be fastened to a ring stand. Mortar and Pestle: Description: Thick glass. Uses: Many uses; Should not be heated Description: Heavy porcelain dish with a grinder. Watch Glass: Uses: To grind chemicals to a powder. Spatula: Description: Curved glass. Uses: May be used as a beaker cover; May be used in evaporating very small amounts of Description: Made of metal or porcelain. liquid. Uses: To transfer solid chemicals in weighing. Funnel: Triangular File: Description: Metal file with three cutting edges. Uses: To scratch glass or file. Rubber Connector: Description: Glass or plastic. Uses: To hold filter paper; May be used in pouring Description: Short length of tubing. Medicine Dropper: Uses: To connect parts of an apparatus. Pinch Clamp: Description: Glass tip with a rubber bulb. Uses: To transfer small amounts of liquid. Forceps: Description: Metal clamp with finger grips. Uses: To clamp a rubber connector. Test Tube Rack: Description: Metal Uses: To pick up or hold small objects. Beaker: Description: Rack; May be wood, metal, or plastic. Uses: To hold test tubes in an upright position. -
Corning Life Sciences Selection Guide Issue 6
www.corning.com/lifesciences Corning Life Sciences Selection Guide Issue 6 Trust Corning for Your Research For superior quality and performance in life science research tools, you can count on Corning. Our comprehensive line of advanced products and technologies is designed to meet your evolving needs in cell-based and genomics research. To learn more, please visit www.corning.com/lifesciences, or call 1.800.492.1110. Customers outside the U.S., please call 1.978.442.2200 or contact your local support office (listed below). Corning Incorporated Worldwide India Taiwan United Kingdom Life Sciences Support Offices t 91-124-235 7850 t 886 2-2716-0338 t 0800 376 8660 f 91-124-401 0207 f 886 2-2716-0339 f 0800 279 1117 Corning BV Life Sciences ASIA/PACIFIC Japan All Other European EUROPE Koolhovenlaan 12 Australia t 81 (0) 3-3586 1996/1997 Countries 1119 NE Schiphol Rijk t 61 2-9416-0492 f 81 (0) 3-3586 1291/1292 France t 31 (0) 20 659 60 51 The Netherlands f 61 2-9416-0493 Korea t 0800 916 882 f 31 (0) 20 659 76 73 f 0800 918 636 Corning Incorporated China t 82 2-796-9500 f 82 2-796-9300 Germany LATINAMERICA Life Sciences t 86 21-3222-4666 t 0800 101 1153 Brasil Tower 2, 4th Floor f 86 21-6288-1575 Singapore f 0800 101 2427 t (55-11) 3089-7419 900 Chelmsford St. Hong Kong t 65 6733-6511 The Netherlands f (55-11) 3167-0700 Lowell, MA 01851 t 852-2807-2723 f 65 6861-2913 Mexico t 800.492.1110 f 852-2807-2152 t 31 20 655 79 28 New! t (52-81) 8158-8400 t 978.442.2200 f 31 20 659 76 73 f (52-81) 8313-8589 f 978.442.2476 www.corning.com/lifesciences Online ordering now available 6/08 6M APC CLS–PSG–001-A4-REV6 Corning, Costar, CellBIND, CellCube, CellSTACK, DNA-BIND, Lambda, Octapette, Spin-X, Stripette, Stripwell, Thermowell, Transtar-96, Transwell,, 8-Pette, and 12-Pette are registered trademarks of Corning Incorporated, Corning, NY. -
Laboratory Supplies and Equipment
Laboratory Supplies and Equipment Beakers: 9 - 12 • Beakers with Handles • Printed Square Ratio Beakers • Griffin Style Molded Beakers • Tapered PP, PMP & PTFE Beakers • Heatable PTFE Beakers Bottles: 17 - 32 • Plastic Laboratory Bottles • Rectangular & Square Bottles Heatable PTFE Beakers Page 12 • Tamper Evident Plastic Bottles • Concertina Collapsible Bottle • Plastic Dispensing Bottles NEW Straight-Side Containers • Plastic Wash Bottles PETE with White PP Closures • PTFE Bottle Pourers Page 39 Containers: 38 - 42 • Screw Cap Plastic Jars & Containers • Snap Cap Plastic Jars & Containers • Hinged Lid Plastic Containers • Dispensing Plastic Containers • Graduated Plastic Containers • Disposable Plastic Containers Cylinders: 45 - 48 • Clear Plastic Cylinder, PMP • Translucent Plastic Cylinder, PP • Short Form Plastic Cylinder, PP • Four Liter Plastic Cylinder, PP NEW Polycarbonate Graduated Bottles with PP Closures Page 21 • Certified Plastic Cylinder, PMP • Hydrometer Jar, PP • Conical Shape Plastic Cylinder, PP Disposal Boxes: 54 - 55 • Bio-bin Waste Disposal Containers • Glass Disposal Boxes • Burn-upTM Bins • Plastic Recycling Boxes • Non-Hazardous Disposal Boxes Printed Cylinders Page 47 Drying Racks: 55 - 56 • Kartell Plastic Drying Rack, High Impact PS • Dynalon Mega-Peg Plastic Drying Rack • Azlon Epoxy Coated Drying Rack • Plastic Draining Baskets • Custom Size Drying Racks Available Burn-upTM Bins Page 54 Dynalon® Labware Table of Contents and Introduction ® Dynalon Labware, a leading wholesaler of plastic lab supplies throughout -
High School Chemistry
RECOMMENDED MINIMUM CORE INVENTORY TO SUPPORT STANDARDS-BASED INSTRUCTION HIGH SCHOOL GRADES SCIENCES High School Chemistry Quantity per Quantity per lab classroom/ Description group adjacent work area SAFETY EQUIPMENT 2 Acid storage cabinet (one reserved exclusively for nitric acid) 1 Chemical spill kit 1 Chemical storage reference book 5 Chemical waste containers (Categories: corrosives, flammables, oxidizers, air/water reactive, toxic) 1 Emergency shower 1 Eye wash station 1 Fire blanket 1 Fire extinguisher 1 First aid kit 1 Flammables cabinet 1 Fume hood 1/student Goggles 1 Goggles sanitizer (holds 36 pairs of goggles) 1/student Lab aprons COMPUTER ASSISTED LEARNING 1 Television or digital projector 1 VGA Adapters for various digital devices EQUIPMENT/SUPPLIES 1 box Aluminum foil 100 Assorted rubber stoppers 1 Balance, analytical (0.001g precision) 5 Balance, electronic or manual (0.01g precision) 1 pkg of 50 Balloons, latex 4 Beakers, 50 mL 4 Beakers, 100 mL 2 Beakers, 250 mL Developed by California Science Teachers Association to support the implementation of the California Next Generation Science Standards. Approved by the CSTA Board of Directors November 17, 2015. Quantity per Quantity per lab classroom/ Description group adjacent work area 2 Beakers, 400 or 600 mL 1 Beakers, 1000 mL 1 Beaker tongs 1 Bell jar 4 Bottle, carboy round, LDPE 10 L 4 Bottle, carboy round, LDPE 4 L 10 Bottle, narrow mouth, 1000 mL 20 Bottle, narrow mouth, 125 mL 20 Bottle, narrow mouth, 250 mL 20 Bottle, narrow mouth, 500 mL 10 Bottle, wide mouth, 125 -
Detergent Residue Testing Using a Ph Meter, Ph Indicator, Or Test Kit
Alconox, Inc. The Leader in Critical Cleaning 30 Glenn St. White Plains NY 10603 USA Tel 914.948.4040 Fax 914-948-4088 www.alconox.com [email protected] Detergent Residue Testing Using a pH Meter, pH indicator, or Test Kit The following test procedures are suitable for detecting detergent residues resulting from improper rinsing and can be used to meet laboratory accreditation guidelines and questionnaires such as the College of American Pathologist program of State water lab accreditation programs. A. pH Meter Method 1. Rinse a small clean beaker by filling and emptying 3 times with source water. 2. Fill a 4th time and measure pH using a pH meter. Record the pH as source water pH. 3. Using a piece of cleaned glassware you wish to test, fill about 10% full with source water (10ml into 100ml beaker). Use more water if necessary to get enough water to be able to sufficiently immerse the pH meter electrode in your measuring beaker. 4. Swish water in glassware to extract residues from all possible surfaces. 5. Take pH reading with pH meter and record as glassware pH. 6. Any significant increase in pH indicates possible alkaline detergent residue. A significant change is 0.2 or more pH units on a pH meter measuring to 0.1 pH units of sensitivity. A result of less than 0.2-pH units change indicates properly rinsed glassware. Note: If deionized water is used as the sample water, a slight amount (10-20 mg/L) of reagent grade, non-buffering salt (NaCl, CaCl2) should be added to the sample water to allow pH meter to function properly.