RPB2 (POLR2B) (NM 001303269) Human Tagged ORF Clone Product Data

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for RC240015

RPB2 (POLR2B) (NM_001303269) Human Tagged ORF Clone Product data:

Product Type: Expression Plasmids Product Name: RPB2 (POLR2B) (NM_001303269) Human Tagged ORF Clone Tag: Myc-DDK Symbol: POLR2B Synonyms: hRPB140; POL2RB; RPB2 Vector: pCMV6-Entry (PS100001) E. coli Selection: Kanamycin (25 ug/mL) Cell Selection: Neomycin ORF Nucleotide >RC240015 representing NM_001303269 Sequence: Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC

ATGCAATATGATGAGGATGATGATGAAATCACCCCGGATTTGTGGCAAGAAGCATGCTGGATTGTAATCA GTTCCTATTTTGACGAGAAAGGCTTGGTTAGACAACAGCTGGATTCTTTTGATGAGTTTATTCAGATGTC TGTTCAAAGAATTGTGGAAGACGCTCCTCCTATAGACCTACAGGCTGAAGCTCAGCATGCTAGTGGAGAA GTTGAAGAACCGCCACGATATTTGCTGAAGTTTGAACAAATTTATCTTTCCAAGCCTACCCATTGGGAAA GAGATGGTGCTCCTTCACCAATGATGCCCAATGAAGCTAGATTAAGGAATCTCACGTATTCTGCTCCGCT TTATGTTGATATAACAAAAACAGTCATTAAAGAAGGTGAAGAACAACTTCAGACTCAGCATCAGAAAACT TTTATAGGAAAAATTCCAATTATGTTGCGGTCAACTTACTGCCTTTTGAATGGCTTGACAGATCGTGATC TTTGTGAGTTAAATGAATGCCCTTTGGATCCTGGTGGCTATTTCATTATTAATGGATCAGAAAAGGTTCT GATTGCCCAAGAGAAAATGGCAACAAACACAGTTTATGTGTTTGCCAAAAAGGATTCTAAATATGCCTAC ACAGGAGAGTGTAGATCATGTCTTGAGAATTCTTCCCGACCCACCAGTACTATATGGGTTAGCATGCTGG CAAGAGGAGGACAGGGTGCCAAGAAGAGTGCTATTGGTCAGCGCATTGTGGCAACTCTACCATATATCAA GCAAGAAGTTCCCATCATTATTGTGTTCAGAGCATTAGGTTTTGTGTCCGACAGAGATATTTTAGAACAT ATTATTTATGATTTTGAAGATCCAGAGATGATGGAAATGGTTAAACCTTCTCTCGATGAAGCTTTTGTCA TCCAAGAACAGAATGTTGCACTAAATTTCATTGGTTCAAGAGGAGCAAAGCCTGGTGTTACTAAAGAGAA AAGAATTAAATATGCAAAGGAAGTTTTACAAAAAGAAATGCTCCCTCATGTTGGTGTCAGTGATTTTTGT GAGACCAAAAAAGCCTATTTCTTGGGATACATGGTTCATAGGTTACTTCTGGCAGCTTTGGGTAGAAGAG AACTAGATGACAGAGATCACTATGGAAACAAGAGATTGGATCTTGCTGGGCCGCTGCTTGCATTCTTATT TAGAGGTATGTTTAAGAATTTGCTTAAAGAAGTGCGGATCTATGCACAGAAATTTATTGATCGAGGAAAG GATTTTAACTTGGAGTTGGCAATTAAAACACGGATCATATCTGATGGCCTAAAATACTCTTTAGCTACTG GAAACTGGGGTGATCAAAAGAAAGCTCATCAAGCCAGAGCTGGAGTATCTCAGGTGTTAAACCGCCTGAC TTTTGCGTCTACTCTTTCTCACCTGCGTCGTTTAAATTCTCCTATTGGTAGAGACGGCAAGCTAGCAAAA CCAAGACAGTTGCATAATACGTTGTGGGGAATGGTGTGTCCTGCCGAGACCCCAGAGGGCCATGCTGTAG

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 5 RPB2 (POLR2B) (NM_001303269) Human Tagged ORF Clone – RC240015

GACTTGTGAAGAATTTAGCCTTGATGGCGTATATTTCAGTTGGATCTCAACCATCTCCAATTCTGGAATT TTTAGAAGAATGGAGTATGGAAAATTTAGAAGAAATTTCTCCTGCAGCTATTGCTGATGCAACCAAGATT TTTGTTAATGGCTGCTGGGTTGGAATACATAAAGATCCCGAACAACTTATGAACACCCTAAGGAAATTGA GACGTCAGATGGACATCATTGTGTCTGAAGTTTCTATGATCAGAGATATTCGAGAGAGGGAGATTCGGAT CTATACGGATGCAGGCCGTATTTGTAGACCACTTCTGATTGTGGAAAAACAAAAGCTACTTTTGAAGAAG AGGCATATTGACCAATTGAAAGAGAGAGAATATAACAACTATAGTTGGCAGGATCTTGTGGCCAGTGGGG TAGTGGAGTATATTGATACCCTGGAAGAAGAAACAGTGATGCTTGCAATGACTCCAGATGATTTACAGGA GAAAGAAGTAGCTTATTGTTCCACATATACACACTGTGAGATTCATCCCTCAATGATCCTTGGTGTCTGT GCATCTATTATTCCCTTTCCTGATCATAACCAGTCCCCTAGAAACACATACCAGTCTGCTATGGGTAAGC AGGCTATGGGAGTTTACATCACCAACTTCCATGTTCGCATGGACACATTGGCCCATGTTCTCTATTATCC TCAAAAGCCACTTGTGACTACACGGTCTATGGAATATCTACGATTTAGAGAGCTGCCAGCAGGCATCAAC TCAATTGTGGCCATTGCATCATACACTGGATATAATCAGGAAGACTCTGTTATCATGAATCGTTCAGCTG TAGACCGCGGCTTCTTCAGGTCTGTTTTCTATCGCTCATACAAAGAACAGGAGTCTAAAAAAGGATTTGA TCAAGAAGAAGTTTTTGAGAAGCCTACACGTGAAACATGCCAGGGCATGAGGCATGCCATTTACGACAAG CTGGATGATGATGGTTTGATAGCTCCAGGGGTTCGTGTATCAGGAGATGATGTTATTATAGGCAAAACAG TCACCTTGCCTGAAAATGAAGATGAATTGGAGAGCACCAATAGACGCTATACCAAGAGAGACTGTAGCAC TTTTCTCAGAACTAGCGAGACGGGCATTGTGGATCAGGTTATGGTAACTCTCAATCAGGAAGGATATAAA TTTTGTAAAATAAGGGTACGCTCTGTTAGGATTCCACAGATTGGAGACAAATTTGCTAGTCGACATGGTC AAAAGGGTACTTGTGGTATTCAGTATAGACAAGAGGATATGCCTTTCACCTGTGAAGGTATCACCCCTGA TATCATCATCAATCCCCATGCCATCCCCTCTCGTATGACTATTGGTCACTTAATTGAATGCCTTCAAGGG AAGGTATCGGCTAACAAGGGTGAAATTGGTGATGCCACTCCATTTAATGATGCTGTTAACGTGCAGAAGA TTTCTAATCTTTTATCTGATTATGGCTATCATCTCAGAGGAAATGAGGTCCTGTACAATGGGTTCACTGG TCGAAAAATCACATCACAAATATTTATTGGCCCCACTTATTACCAGCGTTTGAAGCATATGGTGGATGAT AAGATTCACTCTCGTGCTAGGGGACCTATTCAGATCCTCAATAGACAGCCCATGGAGGGTAGATCTCGTG ATGGTGGCCTGCGTTTTGGAGAAATGGAACGAGATTGTCAGATTGCCCATGGAGCAGCCCAGTTTTTAAG GGAAAGATTGTTTGAGGCATCAGATCCATATCAGGTTCATGTTTGCAATCTTTGTGGAATAATGGCGATT GCCAACACCAGGACCCATACATATGAATGCAGGGGCTGCCGCAATAAAACCCAGATTTCTTTGGTGCGAA TGCCTTACGCATGCAAACTATTGTTTCAGGAACTTATGTCTATGAGTATTGCACCGCGAATGATGAGTGT T

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 2 / 5 RPB2 (POLR2B) (NM_001303269) Human Tagged ORF Clone – RC240015

Protein Sequence: >RC240015 representing NM_001303269 Red=Cloning site Green=Tags(s)

MQYDEDDDEITPDLWQEACWIVISSYFDEKGLVRQQLDSFDEFIQMSVQRIVEDAPPIDLQAEAQHASGE VEEPPRYLLKFEQIYLSKPTHWERDGAPSPMMPNEARLRNLTYSAPLYVDITKTVIKEGEEQLQTQHQKT FIGKIPIMLRSTYCLLNGLTDRDLCELNECPLDPGGYFIINGSEKVLIAQEKMATNTVYVFAKKDSKYAY TGECRSCLENSSRPTSTIWVSMLARGGQGAKKSAIGQRIVATLPYIKQEVPIIIVFRALGFVSDRDILEH IIYDFEDPEMMEMVKPSLDEAFVIQEQNVALNFIGSRGAKPGVTKEKRIKYAKEVLQKEMLPHVGVSDFC ETKKAYFLGYMVHRLLLAALGRRELDDRDHYGNKRLDLAGPLLAFLFRGMFKNLLKEVRIYAQKFIDRGK DFNLELAIKTRIISDGLKYSLATGNWGDQKKAHQARAGVSQVLNRLTFASTLSHLRRLNSPIGRDGKLAK PRQLHNTLWGMVCPAETPEGHAVGLVKNLALMAYISVGSQPSPILEFLEEWSMENLEEISPAAIADATKI FVNGCWVGIHKDPEQLMNTLRKLRRQMDIIVSEVSMIRDIREREIRIYTDAGRICRPLLIVEKQKLLLKK RHIDQLKEREYNNYSWQDLVASGVVEYIDTLEEETVMLAMTPDDLQEKEVAYCSTYTHCEIHPSMILGVC ASIIPFPDHNQSPRNTYQSAMGKQAMGVYITNFHVRMDTLAHVLYYPQKPLVTTRSMEYLRFRELPAGIN SIVAIASYTGYNQEDSVIMNRSAVDRGFFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDK LDDDGLIAPGVRVSGDDVIIGKTVTLPENEDELESTNRRYTKRDCSTFLRTSETGIVDQVMVTLNQEGYK FCKIRVRSVRIPQIGDKFASRHGQKGTCGIQYRQEDMPFTCEGITPDIIINPHAIPSRMTIGHLIECLQG KVSANKGEIGDATPFNDAVNVQKISNLLSDYGYHLRGNEVLYNGFTGRKITSQIFIGPTYYQRLKHMVDD KIHSRARGPIQILNRQPMEGRSRDGGLRFGEMERDCQIAHGAAQFLRERLFEASDPYQVHVCNLCGIMAI ANTRTHTYECRGCRNKTQISLVRMPYACKLLFQELMSMSIAPRMMSV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV Restriction Sites: SgfI-MluI Cloning Scheme:

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 3 / 5 RPB2 (POLR2B) (NM_001303269) Human Tagged ORF Clone – RC240015

Plasmid Map:

ACCN: NM_001303269 ORF Size: 3501 bp OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. RefSeq: NM_001303269.1, NP_001290198.1

RefSeq Size: 4240 bp RefSeq ORF: 3504 bp Locus ID: 5431 UniProt ID: P30876, C9J2Y9, B4DHJ3, A0A1W2PPT5, B4DH29 Protein Families: Transcription Factors Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase MW: 133.5 kDa

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 4 / 5 RPB2 (POLR2B) (NM_001303269) Human Tagged ORF Clone – RC240015

Gene Summary: This gene encodes the second largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase that catalyzes the transcription of DNA into precursors of mRNA, snRNA and microRNA. This subunit and the largest subunit form opposite sides of the center cleft of Pol II. Deletion of the flap loop region of this subunit results in a decrease in the rate of transcriptional elongation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]