Anti-JAM3 Monoclonal Antibody, Clone DSBN-29 G37 [FITC] (DCABY-713) This Product Is for Research Use Only and Is Not Intended for Diagnostic Use

Anti-JAM3 monoclonal antibody, clone DSBN-29 G37 [FITC] (DCABY-713) This product is for research use only and is not intended for diagnostic use.

PRODUCT INFORMATION

Antigen Description JAM-C is expressed at junctions between endothelial and epithelial cells, as well as on leukocytes, platelets, vascular smooth muscle cells and fibroblasts, amongst other cell types. It plays a role in tight junctions and inflammatory processes and interacts with JAM-A and JAM- B.Clone DSBN-29 G37 has been reported to inhibit transendothelial migration. We recommends using this product for this purpose.

Specificity Clone DSBN-29 G37 recognises mouse and human Junctional adhesion molecule C (JAM-C), also known as JAM-3 and, historically, as JAM-2.

Immunogen Recombinant soluble JAM-C

Isotype IgG2a

Source/Host Rat

Species Reactivity Mouse, Human

Clone DSBN-29 G37

Conjugate FITC

Applications Flow Cytometry, Immunofluorescence

Preparation Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Procedure Conjugated Antibodies

Format Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Size 100 μg

Buffer Phosphate buffered saline

Preservative None

Storage Store at +4°C or at -20°C if preferred.Storage in frost-free freezers is not recommended.This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]

Gene Name Jam3 junction adhesion molecule 3 [ Mus musculus (house mouse) ]

Official Symbol Jam3

Synonyms Jam3; junction adhesion molecule 3; JAM-3; JAM-C; Jcam3; 1110002N23Rik; junctional adhesion molecule C; junctional adhesion molecule 3; junction cell adhesion molecule 3; junction cell adhesion molecule C

Entrez Gene ID 83964

Protein Refseq NP_075766

UniProt ID Q9D8B7

Chromosome Location 9 A4; 9

Pathway Cell adhesion molecules (CAMs); Cell surface interactions at the vascular wall; Extracellular matrix organization; Hemostasis; Integrin cell surface interactions; Leukocyte transendothelial migration; Tight junction; XPodNet - protein-protein interactions in the podocyte expanded by STRING

Function integrin binding;integrin binding;protein binding;protein heterodimerization activity;protein homodimerization activity

References 1. Aurrand-Lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members. Blood. 98: 3699 - 3707. 2. Johnson-Léger, C.A. et al. (2002) Junctional adhesion molecule - 2 (JAM-2) promotes lymphocyte transendothelial migration. Blood. 100: 2479 - 2486. 3. Aurrand-Lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells. J. Biol. Chem. 276: 2733 - 2741. 4. Forsberg, E.C. et al. (2005) Differential expression of novel potential regulators in hematopoietic stem cells.PLoS Genet. 1(3):e28.

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]