DOCSLIB.ORG

HOX Genes in Pathogenesis of Ewing Sarcoma

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
HOX Genes in Pathogenesis of Ewing Sarcoma Fakultät für Medizin HOX genes in pathogenesis of Ewing sarcoma Miriam Verena Manuela Ertl Vollständiger Abdruck der von der Fakultät für Medizin der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Medizin genehmigten Dissertation. Vorsitzender: Prof. Dr. Ernst J. Rummeny Prüfer der Dissertation: 1. Prof. Dr. Stephanie Elisabeth Combs 2. Prof. Dr. Stefan Burdach Die Dissertation wurde am 25.09.2017 bei der Technischen Universität München eingereicht und durch die Fakultät für Medizin am 02.05.2018 angenommen. meiner Mutter TABLE OF CONTENTS Table of contents List of abbreviations ......................................................................................................... III 1 Introduction ...................................................................................... 1 1.1 HOX genes......................................................................................................... 1 1.2 Skeletogenesis and bone formation ............................................................... 15 1.3 Bone morphogenetic proteins and the TGF β pathway ................................. 19 1.4 Ewing sarcoma ................................................................................................ 22 1.5 Aim of this study and experimental approach ............................................... 28 2 Materials and methods ....................................................................29 2.1 Materials ......................................................................................................... 29 2.2 Methods.......................................................................................................... 43 3 Results .............................................................................................63 3.1 Posterior HOXD genes are overexpressed in Ewing sarcoma ........................ 63 3.2 HOX gene expression is not regulated by the two “main suspects” .............. 67 3.3 HOX gene expression is downregulated by siRNA interference .................... 71 3.4 HOX gene expression is downregulated by retroviral gene transfer ............. 74 3.5 Microarray analysis reveals possible downstream HOX targets .................... 75 3.6 HOX genes seem to intervene in BMP signaling in ES cells ............................ 80 3.7 HOX genes don’t influence endothelial cell tube formation .......................... 83 3.8 HOX genes promote proliferation of Ewing sarcoma cells ............................ 84 3.9 HOX knockdown inhibits anchorage-independent growth of ES cells ........... 86 3.10 HOX genes promote chondrogenic differentiation in Ewing sarcoma cells ... 87 3.11 Ewing sarcoma cells are capable of osteogenic differentiation ..................... 90 4 Discussion ........................................................................................95 4.1 Overexpression of HOXD10, HOXD11 and HOXD13 in Ewing sarcoma ......... 95 4.2 HOX gene expression is not regulated by EZH2 in Ewing sarcoma .............. 100 4.3 HOX gene expression is not regulated by EWS-FLI1 .................................... 102 4.4 Microarray analysis reveals few but critical downstream targets ............... 105 I 4.5 HOX knockdown impairs proliferation and anchorage-independent growth of Ewing sarcoma cells ..................................................................... 112 4.6 HOX genes control genes related to BMP signaling and are critical for chondrogenic differentiation of Ewing sarcoma cells .................................. 115 4.7 Assumed role of HOX genes in pathogenesis of Ewing sarcoma ................. 126 4.8 Clinical implications and future perspectives............................................... 126 5 Summary ...................................................................................... 129 6 Zusammenfassung ........................................................................ 131 7 References .................................................................................... 133 8 Publication .................................................................................... 161 9 Appendices ................................................................................... 163 9.1 List of figures ................................................................................................ 163 9.2 List of tables .................................................................................................. 164 9.3 Amino acids and their codes ........................................................................ 165 10 Acknowledgements ....................................................................... 167 II LIST OF ABBREVIATIONS List of abbreviations Abd-A Abdominal A; component of Bithorax complex Abd-B Abdominal B; component of Bithorax complex ACTB β-actin AGC1 Aggrecan 1 ALL Acute lymphoid leukemia ALP Alkalic phosphatase ALPL Alkalic phosphatase AMH Anti-Muellerian hormone AML Acute myeloid leukemia Antp Antennapedia; component of Antennapedia complex Arg Arginine Asn Asparagine α thalassamia/mental retardation syndrome x-linked; also known as ATRX XNP B3GAT1 β-1,3-glucuronyltransferase 1; also known as CD57, LEU7 and HNK1 BAMBI BMP and activin membrane bound inhibitor BCP 1-bromo-3-chloropropane BGLAP Bone γ-carboxyglutamate protein; also known as osteocalcin BHA Butylated hydroxyanisole BMI1 proto-oncogene, polycomb ring finger protein; also known as BMI1 PCGF4; component of PRC1 BMP Bone morphogenetic protein BMP2 Bone morphogenetic protein 2 BMP4 Bone morphogenetic protein 4 BMP7 Bone morphogenetic protein 7 BMPs Bone morphogenetic proteins bp Base pair BSA Bovine serum albumine BSP Bone sialoprotein CBFα1 Core binding factor α1; also known as RUNX2 CBX Component of PRC1 CCD Cleidocranial dysplasia CD49 Certain cell surface glycoprotein; lymphoid marker CD57 β-1,3-glucuronyltransferase 1; also known as B3GAT1, LEU7 and HNK1 III CD99 Certain cell surface glycoprotein; also known as MIC2 cDNA Complementary DNA CHM1 Chondromodulin 1 CNS Central nervous system COL10A1 Collagen type X alpha 1 COL1A1 Collagen type I alpha 1 COL2A1 Collagen type II alpha 1 Certain DNA region where cytosine and guanine are separated by only CpG site one phosphate CT Computed tomography CTEV Congenital talipes equinovarus DEPC Diethylpyrocarbonate Dfd Deformed; component of Antennapedia complex DKK2 Dickkopf 2 DMSO Dimethyl sulfoxide DNA Deoxyribonucleic acid dNTPs Deoxynucleotides; nucleoside triphosphates DPBS Dulbecco’s phosphate-buffered saline ds Double strand ECM Extracellular matrix EDTA Ethylenediaminetetraacetic acid EED Embryonic ectoderm development EEF1D Eukaryotic translation elongation factor 1 delta EFT Ewing family of tumors EGF Epidermal growth factor Engelbreth-Holm-Swarm; name of a mouse tumor with abundant EHS extracellular matrix ENT Ear, nose and throat ETS-related gene; full name: v-ets avian erythroblastosis virus E26 ERG oncogene homolog ERK Extracellular signal-regulated kinase ES Ewing sarcoma ETS E26 family of transcription factors ETS E26 transformation-specific gene; encodes transcription factor ETV1 ETS variant 1 ETV4 ETS variant 4, also called E1AF IV LIST OF ABBREVIATIONS EWS Ewing sarcoma gene EWS-FLI1 Fusion protein which characterizes Ewing sarcomas EWSR1 Ewing sarcoma breakpoint region 1 EWSR2 Ewing sarcoma breakpoint region 2 EZH1 Enhancer of zeste homolog 1; polycomb repressive complex 2 subunit EZH2 Enhancer of zeste homolog 2; polycomb repressive complex 2 subunit FAM™ 6-carboxyfluorescein FDG-PET fluorine-18 fluorodeoxyglucose positron emission tomography FEV Gene in fifth Ewing sarcoma variant FGF Fibroblast growth factor FISH Fluorescence in situ hybridization FLI1 Friend leukemia virus integration site 1 GDF Growth differentiation factor gDNA Genomic DNA GPR64 G protein-coupled receptor 64 GTP Guanosine triphosphate HAUS6 HAUS augmin-like complex, subunit 6 HCl Hydrogen chloride HNK1 β-1,3-glucuronyltransferase 1; also known as B3GAT1, LEU7 and CD57 HOTAIR Acronym of a certain RNA called HOX Antisense Intergenic RNA HOX genes Homeotic genes HOXA13 Homeobox A13 HOXD10 Homeobox D10 HOXD11 Homeobox D11 HOXD13 Homeobox D13 HPH Component of PRC1 IBSP Integrin binding sialoprotein; also known as BSP IGF Insulin-like growth factor IGFBP3 Insulin-like growth factor binding protein 3 IHH Indian hedgehog I-SMAD Inhibitory SMAD JNK c-Jun NH2-terminal kinase kb Kilobase Lab Labial; component of Antennapedia complex LEU7 β-1,3-glucuronyltransferase 1; also known as B3GAT1, HNK1 and CD57 V MAP2K MAPK kinase MAP3K MAPK kinase kinase MAPK Mitogen-activated protein kinase MDS Myelodysplastic syndrome MEIS Family of proteins; group of homeobox genes MGB Minor groove binder; name of a certain quencher MIC2 Certain cell surface glycoprotein which is also known as CD99 min Minutes miR MicroRNA miR-10b MicroRNA 10b miR-224 MicroRNA 224 miR-23a MicroRNA 23a miR-7 MicroRNA 7 miRNA MicroRNA MMP Matrix metalloproteinase MMP13 Matrix metallopeptidase 13 MRI Magnetic resonance imaging mRNA Messenger RNA MSC Mesenchymal stem cell n.d. Not detectable n.s. Not significant NHL Non-Hodgkin lymphoma NMR Nuclear magnetic resonance NSE Neuron-specific enolase NTC Non-template control ON Osteonectin OP1 Also known as BMP7 OSX Also known as SP7 PAK1 p21-activated kinase 1 Pb Proboscipedia; component of Antennapedia complex PBS Phosphate-buffered saline PBX PBX homeobox protein PcG Polycomb-group PCGF Certain polycomb group protein; component of PRC1 PCGF4 Also known as BMI1; polycomb ring finger protein; component
Load more

Recommended publications
  • New York State Thoroughbred Breeding and Development Fund Corporation
    NEW YORK STATE THOROUGHBRED BREEDING AND DEVELOPMENT FUND CORPORATION Report for the Year 2008 NEW YORK STATE THOROUGHBRED BREEDING AND DEVELOPMENT FUND CORPORATION SARATOGA SPA STATE PARK 19 ROOSEVELT DRIVE-SUITE 250 SARATOGA SPRINGS, NY 12866 Since 1973 PHONE (518) 580-0100 FAX (518) 580-0500 WEB SITE http://www.nybreds.com DIRECTORS EXECUTIVE DIRECTOR John D. Sabini, Chairman Martin G. Kinsella and Chairman of the NYS Racing & Wagering Board Patrick Hooker, Commissioner NYS Dept. Of Agriculture and Markets COMPTROLLER John A. Tesiero, Jr., Chairman William D. McCabe, Jr. NYS Racing Commission Harry D. Snyder, Commissioner REGISTRAR NYS Racing Commission Joseph G. McMahon, Member Barbara C. Devine Phillip Trowbridge, Member William B. Wilmot, DVM, Member Howard C. Nolan, Jr., Member WEBSITE & ADVERTISING Edward F. Kelly, Member COORDINATOR James Zito June 2009 To: The Honorable David A. Paterson and Members of the New York State Legislature As I present this annual report for 2008 on behalf of the New York State Thoroughbred Breeding and Development Fund Board of Directors, having just been installed as Chairman in the past month, I wish to reflect on the profound loss the New York racing community experienced in October 2008 with the passing of Lorraine Power Tharp, who so ably served the Fund as its Chairwoman. Her dedication to the Fund was consistent with her lifetime of tireless commitment to a variety of civic and professional organizations here in New York. She will long be remembered not only as a role model for women involved in the practice of law but also as a forceful advocate for the humane treatment of all animals.
    [Show full text]
  • Exemplar Texts for Grades
    COMMON CORE STATE STANDARDS FOR English Language Arts & Literacy in History/Social Studies, Science, and Technical Subjects _____ Appendix B: Text Exemplars and Sample Performance Tasks OREGON COMMON CORE STATE STANDARDS FOR English Language Arts & Literacy in History/Social Studies, Science, and Technical Subjects Exemplars of Reading Text Complexity, Quality, and Range & Sample Performance Tasks Related to Core Standards Selecting Text Exemplars The following text samples primarily serve to exemplify the level of complexity and quality that the Standards require all students in a given grade band to engage with. Additionally, they are suggestive of the breadth of texts that students should encounter in the text types required by the Standards. The choices should serve as useful guideposts in helping educators select texts of similar complexity, quality, and range for their own classrooms. They expressly do not represent a partial or complete reading list. The process of text selection was guided by the following criteria: Complexity. Appendix A describes in detail a three-part model of measuring text complexity based on qualitative and quantitative indices of inherent text difficulty balanced with educators’ professional judgment in matching readers and texts in light of particular tasks. In selecting texts to serve as exemplars, the work group began by soliciting contributions from teachers, educational leaders, and researchers who have experience working with students in the grades for which the texts have been selected. These contributors were asked to recommend texts that they or their colleagues have used successfully with students in a given grade band. The work group made final selections based in part on whether qualitative and quantitative measures indicated that the recommended texts were of sufficient complexity for the grade band.
    [Show full text]
  • Tramping Through Mexico, Guatemala and Honduras - Being the Random Notes of an Incurable Vagabond
    Tramping Through Mexico, Guatemala and Honduras - Being the Random Notes of an Incurable Vagabond Harry A. Franck The Project Gutenberg EBook of Tramping Through Mexico, Guatemala and Honduras, by Harry A. Franck #2 in our series by Harry A. Franck Copyright laws are changing all over the world. Be sure to check the copyright laws for your country before downloading or redistributing this or any other Project Gutenberg eBook. This header should be the first thing seen when viewing this Project Gutenberg file. Please do not remove it. Do not change or edit the header without written permission. Please read the "legal small print," and other information about the eBook and Project Gutenberg at the bottom of this file. Included is important information about your specific rights and restrictions in how the file may be used. You can also find out about how to make a donation to Project Gutenberg, and how to get involved. **Welcome To The World of Free Plain Vanilla Electronic Texts** **eBooks Readable By Both Humans and By Computers, Since 1971** *****These eBooks Were Prepared By Thousands of Volunteers!***** Title: Tramping Through Mexico, Guatemala and Honduras Being the Random Notes of an Incurable Vagabond Author: Harry A. Franck Release Date: December, 2004 [EBook #7072] [Yes, we are more than one year ahead of schedule] [This file was first posted on March 6, 2003] Edition: 10 Language: English Character set encoding: ASCII *** START OF THE PROJECT GUTENBERG EBOOK TRAMPING IN MEXICO *** This eBook was produced by Jim O'Connor, Charles Aldarondo, Charles Franks and the Online Distributed Proofreading Team Livros Grátis http://www.livrosgratis.com.br Milhares de livros grátis para download.
    [Show full text]
  • Filozofické Aspekty Technologií V Komediálním Sci-Fi Seriálu Červený Trpaslík
    Masarykova univerzita Filozofická fakulta Ústav hudební vědy Teorie interaktivních médií Dominik Zaplatílek Bakalářská diplomová práce Filozofické aspekty technologií v komediálním sci-fi seriálu Červený trpaslík Vedoucí práce: PhDr. Martin Flašar, Ph.D. 2020 Prohlašuji, že jsem tuto práci vypracoval samostatně a použil jsem literárních a dalších pramenů a informací, které cituji a uvádím v seznamu použité literatury a zdrojů informací. V Brně dne ....................................... Dominik Zaplatílek Poděkování Tímto bych chtěl poděkovat panu PhDr. Martinu Flašarovi, Ph.D za odborné vedení této bakalářské práce a podnětné a cenné připomínky, které pomohly usměrnit tuto práci. Obsah Úvod ................................................................................................................................................. 5 1. Seriál Červený trpaslík ................................................................................................................... 6 2. Vyobrazené technologie ............................................................................................................... 7 2.1. Android Kryton ....................................................................................................................... 14 2.1.1. Teologická námitka ........................................................................................................ 15 2.1.2. Argument z vědomí ....................................................................................................... 18 2.1.3. Argument z
    [Show full text]
  • Leaves of Grass
    Leaves of Grass by Walt Whitman AN ELECTRONIC CLASSICS SERIES PUBLICATION Leaves of Grass by Walt Whitman is a publication of The Electronic Classics Series. This Portable Document file is furnished free and without any charge of any kind. Any person using this document file, for any pur- pose, and in any way does so at his or her own risk. Neither the Pennsylvania State University nor Jim Manis, Editor, nor anyone associated with the Pennsylvania State University assumes any responsibility for the material contained within the document or for the file as an electronic transmission, in any way. Leaves of Grass by Walt Whitman, The Electronic Clas- sics Series, Jim Manis, Editor, PSU-Hazleton, Hazleton, PA 18202 is a Portable Document File produced as part of an ongoing publication project to bring classical works of literature, in English, to free and easy access of those wishing to make use of them. Jim Manis is a faculty member of the English Depart- ment of The Pennsylvania State University. This page and any preceding page(s) are restricted by copyright. The text of the following pages are not copyrighted within the United States; however, the fonts used may be. Cover Design: Jim Manis; image: Walt Whitman, age 37, frontispiece to Leaves of Grass, Fulton St., Brooklyn, N.Y., steel engraving by Samuel Hollyer from a lost da- guerreotype by Gabriel Harrison. Copyright © 2007 - 2013 The Pennsylvania State University is an equal opportunity university. Walt Whitman Contents LEAVES OF GRASS ............................................................... 13 BOOK I. INSCRIPTIONS..................................................... 14 One’s-Self I Sing .......................................................................................... 14 As I Ponder’d in Silence...............................................................................
    [Show full text]
  • Smart Materials for Smart Living
    MATERIALS SCIENCE AND TECHNOLOGIES SMART MATERIALS FOR SMART LIVING No part of this digital document may be reproduced, stored in a retrieval system or transmitted in any form or by any means. The publisher has taken reasonable care in the preparation of this digital document, but makes no expressed or implied warranty of any kind and assumes no responsibility for any errors or omissions. No liability is assumed for incidental or consequential damages in connection with or arising out of information contained herein. This digital document is sold with the clear understanding that the publisher is not engaged in rendering legal, medical or any other professional services. MATERIALS SCIENCE AND TECHNOLOGIES Additional books in this series can be found on Nova’s website under the Series tab. Additional e-books in this series can be found on Nova’s website under the eBooks tab. MATERIALS SCIENCE AND TECHNOLOGIES SMART MATERIALS FOR SMART LIVING RADHESHYAM RAI EDITOR Copyright © 2017 by Nova Science Publishers, Inc. All rights reserved. No part of this book may be reproduced, stored in a retrieval system or transmitted in any form or by any means: electronic, electrostatic, magnetic, tape, mechanical photocopying, recording or otherwise without the written permission of the Publisher. We have partnered with Copyright Clearance Center to make it easy for you to obtain permissions to reuse content from this publication. Simply navigate to this publication’s page on Nova’s website and locate the “Get Permission” button below the title description. This button is linked directly to the title’s permission page on copyright.com.
    [Show full text]
  • Red Dwarf” - by Lee Russell
    Falling in love with “Red Dwarf” - by Lee Russell It was around 2003 or 2004 when I fell in love with the British sci-fi comedy show “Red Dwarf”. I had been somewhat aware of the series when it launched in 1988 but the external model shots looked so unrealistic that I didn’t bother to try it… what a mistake! My epiphany came late one night after a long session of distance-studying for a degree with the Open University. Feeling very tired and just looking for something to relax with before going to bed, I was suddenly confronted with one of the funniest comedy scenes I had ever seen. That scene was in the Season VIII episode ‘Back in the Red, part 2’. I tuned in just at the moment that Rimmer was using a hammer to test the anaesthetic that he’d applied to his nether regions. I didn’t know the character or the back story that had brought him to that moment, but Chris Barrie’s wonderful acting sucked me in – I was laughing out loud and had suddenly become ‘a Dwarfer’. With one exception, I have loved every series of Red Dwarf, and in this blog I’ll be reflecting on what has made me come to love it so much over the 12 series that have been broadcast to date. For anyone was hasn’t seen Red Dwarf (and if you haven’t, get out there and find a copy now – seriously), the story begins with three of the series’ main characters who have either survived, or are descended from, a radiation accident that occurred three million years ago and killed all of the rest of the crew of the Jupiter Mining Corp ship ‘Red Dwarf’.
    [Show full text]
  • The Wisdom of Father Brown, by G. K. Chesterton
    The Wisdom Of Father Brown G. K. Chesterton To Lucian Oldershaw The Absence of Mr Glass The consulting-rooms of Dr Orion Hood, the eminent criminologist and specialist in certain moral disorders, lay along the sea-front at Scarborough, in a series of very large and well-lighted french windows, which showed the North Sea like one endless outer wall of blue-green marble. In such a place the sea had something of the monotony of a blue-green dado: for the chambers themselves were ruled throughout by a terrible tidiness not unlike the terrible tidiness of the sea. It must not be supposed that Dr Hood’s apartments excluded luxury, or even poetry. These things were there, in their place; but one felt that they were never allowed out of their place. Luxury was there: there stood upon a special table eight or ten boxes of the best cigars; but they were built upon a plan so that the strongest were always nearest the wall and the mildest nearest the window. A tantalus containing three kinds of spirit, all of a liqueur excellence, stood always on this table of luxury; but the fanciful have asserted that the whisky, brandy, and rum seemed always to stand at the same level. Poetry was there: the left-hand corner of the room was lined with as complete a set of English classics as the right hand could show of English and foreign physiologists. But if one took a volume of Chaucer or Shelley from that rank, its absence irritated the mind like a gap in a man’s front teeth.
    [Show full text]
  • The Wolf Biermann Story
    University of Montana ScholarWorks at University of Montana Graduate Student Theses, Dissertations, & Professional Papers Graduate School 1980 There is a life before death: The Wolf Biermann story John Shreve The University of Montana Follow this and additional works at: https://scholarworks.umt.edu/etd Let us know how access to this document benefits ou.y Recommended Citation Shreve, John, "There is a life before death: The Wolf Biermann story" (1980). Graduate Student Theses, Dissertations, & Professional Papers. 5262. https://scholarworks.umt.edu/etd/5262 This Thesis is brought to you for free and open access by the Graduate School at ScholarWorks at University of Montana. It has been accepted for inclusion in Graduate Student Theses, Dissertations, & Professional Papers by an authorized administrator of ScholarWorks at University of Montana. For more information, please contact [email protected]. COPYRIGHT ACT OF 1976 Th is is an unpublished m a n u s c r ip t in w h ic h c o p y r ig h t sub ­ s i s t s . Any further r e p r in t in g of it s contents must be approved BY THE AUTHOR. Ma n s f ie l d L ib r a r y Un iv e r s it y o f-M ontana D ate: 198 0 THERE IS A LIFE BEFORE DEATH THE WOLF BIERMANN STORY by John Shreve B.A., University of Montana, 1976 Presented 1n p a rtial fu lfillm e n t of the requirements fo r the degree of Master of Arts UNIVERSITY OF MONTANA 1980 Approved by: & Chairman, Board of Examiners Deari% Graduate Scho6T S'-U- S'o Date UMI Number: EP40726 All rights reserved INFORMATION TO ALL USERS The quality of this reproduction is dependent upon the quality of the copy submitted.
    [Show full text]
  • Hiram Poetry Review Issue #82 Spring 2021 2 the Hiram Poetry Review
    Hiram Poetry Review Issue #82 Spring 2021 2 The Hiram Poetry Review 3 THE HIRAM POETRY REVIEW ISSN 0018-2036 Indexed in American Humanities Index Submission Guidelines: 3-5 poems, SASE. HPR, P.O. Box 162, Hiram, Ohio 44234 [email protected] hirampoetryreview.wordpress.com $9.00 for one year or $23.00 for three years 4 THE HIRAM POETRY REVIEW Issue No. 82 Spring 2021 Editor: Willard Greenwood Associate Editor: Mary Quade Editorial Assistants: Kerry Hamilton, Lauren Hildum, and Quinn Tucker Cover Photo by: Lauren Hildum CONTENTS Editor’s Note 8 David Adams 23-South 9 Anthony Aguero Effigy of my Drug Dealer 11 Fred Arroyo Old Manuel 12 Zulfa Arshad Mango Peels 13 Just a Moment 14 Enne Baker Cystoscopy 15 Grace Bauer Ms. Schadenfreude on Pestilence 16 Demetrius Buckley Coming Back Tonight 17 Jim Daniels TESTAMENT 19 Edmund Dempsey Middle of the Road 21 Norah Esty Fishing, Thirty Years Later 22 Five Bottles of Champagne 24 5 Jess Falkenhagen Never Again 25 Antony Fangary dear u.s. 26 Dan Grote The Creative Process 27 Anvesh Jain Bagah Border, at Age 11 28 Charles Kell Saxifraga 30 Robert McCracken Untitled 33 R. McSwain Normal 34 Mycah Miller Honeycomb Queer 35 Cecil Morris Saying Goodbye 36 Daniel Morris Another Virus 37 James Nnaji Black Crude Poured Again 40 David Sapp Aristocracy 42 John Saad Honeymoon, Dodger Stadium 43 Denzel Scott Garland of Delphinium 44 Bonnie Stanard Conditioned to Impress 45 6 Pamela Sumners The Caretakers 47 Review Charles Parsons Standing Downstream: a review of Everything Comes Next (Greenwillow Books) by Naomi Shihab Nye 49 Contributors’ Notes 52 7 EDITOR’S NOTE Welcome to our second pandemic issue.
    [Show full text]
  • Alamance Co. Birth Certificates Pre 1915
    1 Alamance Co. Birth Certificates pre 1915 Name Birth Yr Father Mother Abernathy, Andrew Jackson 1905 Abernathy, Franklin Jason Massey, Cora Lee Adams, Civella 1903 Adams, Junius Jackson Galloway, Maggie Etta Adams, Joseph Vernie 1899 Adams, James Lawson Whitt, Lavenia Adams, Philip Chappell 1898 Adams, Samuel Lee Mitchell, Sarah Alice Albright, Agnes Mae 1888 Albright, Thomas Alexander Hunter, Margaret Albright, Annie Grace 1897 Albright, Wm. A.* Wilson, Emma Ann Albright, Boy 1914 Albright, Minter A. Albright, Conley Hunter 1899 Albright, Thomas Alexander Hunter, Margaret Albright, Delma Roosevelt 3/24/1911 Albright, Haywood Albright, Minnie Bell Albright, Dolly L. 1904 Albright, Sidney B.* Coble, Lecky Albright, Edward Everett 1909 Albright, Cicero Harris Staton, Lula Estelle Albright, Ethel Mae 1902 Albright, Stephen Daniel Anderson, Ida Jane Albright, Eula Louvenia 1907 Albright, James Samuel Pickard, Louvenia Albright, Fannie Mozella 1906 Albright, Sidney Coble, Lectkey Albright, Flora M. 1881 Albright, Andrew Jackson Vestal, Cornelia Albright, Florence Catherine 1912 unknown Albright, Josie Albright, George Edwin 1904 Albright, Cad Alexander Coble, Emma Albright, George Irving 1903 Albright, Geo. Alonzo Pritchett, Vera Irene Albright, George Thomas 1887 Albright, George Ethridge, Sarah Albright, Grace Elizabeth 1910 Albright, Eugene Lynch, Rachel Albright, Inez Gladys 1891 Albright, Thomas Alexander Hunter, Margaret Albright, Iola 1886 Albright, Andrew Jackson Vestal, Susan Albright, James Cicero 1884 Albright, Andrew Jackson Vestal, Susan Albright, James Howard 1909 Albright, James Samuel Pickard, Louvenia Albright, James R, 1890 Albright, Jerry White, Sarah Albright, Jewell Adare 1903 Albright, Thomas Franklin* Albright, Sarah Ann Albright, Katie Clara 1905 Albright, James Samuel Pickard, Louvenia Albright, Katie Lee 1905 Albright, Sandy Capps, Lillie Albright, Lesta 1902 Albright, Caldwell Alexander Coble, Emma D.
    [Show full text]
  • Rhode Island and the Slave Trade
    ""',*H"f",::lffii,ii#I**J'"#"{,"}NJrihs'orB Deoember2t'2002 ofl-"ll'**. ^on t1g l€nd*-; oz9a6 T}'il:i; DearMember: ;,}';fi,.rJ*s*-*,s*$ilt ?:i;":k'u" --*g"m':+g'# uffry.- 'twrt* Preeident SincerelY' n Roqer N'BeSc F,x"cutivealfrector -nb-'.*LYfo,*/ ' - Bernatu 'D Fishrnarr ?Tlfr["";o""' FALL 2OO2 Rhode Island History ffi Volume 60, Number 4 Published by CONTENTS The Rhode Island Historical Society I l0 Benevolent Street Providence, Rhode Island 02906-3 1 52 and printed by a grant from the State of Rhode Island and the Slave Tiade 9s Rhode Island and Providence Plantations, STANLEY LEMONS Lilcoln Almond, Governor; ]. Edward S. Inman III, Secretary of State Roger N. Begin, president Diana L. lohnson, vice president Writings on Rhode Island History, 105 l Bradford B. Kopp, vice president for development ROGER PARKS Barbara F. Weaver, vice president for libraries I Edward F. Sanderson, vice president for museums d David S. Lu, secretary { C. June Tow, assistant secretary Lyle S. Fain, treasurer Index to Volume5$..'.. t07 Tobias M. Lederberg, assistant treasurer Bernard P. Fishman, director fames K. Mahoney, administrative director FELLOWS OF THE SOCIETY Carl Bridenbaugh T Glenn W. LaFantasie PUBIICATIONS COMMITTEE Harrison M. Wright, chalr lo,vce M. Botelho James Findlay Robert Allen Greene I Robert W. Hayman Jane Lancaster James P Marusak \tilliam McKenzie Woodward ST-\FF \Iarta Y. Martinez, edito r Hilliard Beller, copy editor .{ilison C1vin, graphics editor The Rhode Island Historical Society assumes no responsibility for the :i opidons of contributors. O 2002 by The Rhode Island Historical Society RHODE ISLAND HISTORY (USPS no.
    [Show full text]