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Recombinant E. Coli Lon Protease (C-6His)
9853 Pacific Heights Blvd. Suite D. San Diego, CA 92121, USA Tel: 858-263-4982 Email: [email protected] 32-7101: Recombinant E. coli Lon Protease (C-6His) Gene : lon Gene ID : 945085 Uniprot ID : P0A9M0 Description Source: E.coli. MW :88.6kD. Recombinant E.coli Lon Protease is produced by our E.coli expression system and the target gene encoding Asn2-Lys784 is expressed with a 6His tag at the C-terminus. Lon Protease, is a member of the Lon protease family. They are found in archaea, bacteria and eukaryotes. Lon protease is ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. It required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. It degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long and binds to DNA in a double-stranded, site- specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, and UmuD. Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB- YefM toxin-antitoxin system. Product Info Amount : 10 µg / 50 µg Content : Supplied as a 0.2 µm filtered solution of 50mM TrisHCl, 100mM KCl, 10% Glycerol, pH 7.5 . Storage condition : Store at -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles. Amino Acid : MGNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAMDHDKKIMLVAQKEASTDEPGVN -
(12) Patent Application Publication (10) Pub. No.: US 2006/0110747 A1 Ramseier Et Al
US 200601 10747A1 (19) United States (12) Patent Application Publication (10) Pub. No.: US 2006/0110747 A1 Ramseier et al. (43) Pub. Date: May 25, 2006 (54) PROCESS FOR IMPROVED PROTEIN (60) Provisional application No. 60/591489, filed on Jul. EXPRESSION BY STRAIN ENGINEERING 26, 2004. (75) Inventors: Thomas M. Ramseier, Poway, CA Publication Classification (US); Hongfan Jin, San Diego, CA (51) Int. Cl. (US); Charles H. Squires, Poway, CA CI2O I/68 (2006.01) (US) GOIN 33/53 (2006.01) CI2N 15/74 (2006.01) Correspondence Address: (52) U.S. Cl. ................................ 435/6: 435/7.1; 435/471 KING & SPALDING LLP 118O PEACHTREE STREET (57) ABSTRACT ATLANTA, GA 30309 (US) This invention is a process for improving the production levels of recombinant proteins or peptides or improving the (73) Assignee: Dow Global Technologies Inc., Midland, level of active recombinant proteins or peptides expressed in MI (US) host cells. The invention is a process of comparing two genetic profiles of a cell that expresses a recombinant (21) Appl. No.: 11/189,375 protein and modifying the cell to change the expression of a gene product that is upregulated in response to the recom (22) Filed: Jul. 26, 2005 binant protein expression. The process can improve protein production or can improve protein quality, for example, by Related U.S. Application Data increasing solubility of a recombinant protein. Patent Application Publication May 25, 2006 Sheet 1 of 15 US 2006/0110747 A1 Figure 1 09 010909070£020\,0 10°0 Patent Application Publication May 25, 2006 Sheet 2 of 15 US 2006/0110747 A1 Figure 2 Ester sers Custer || || || || || HH-I-H 1 H4 s a cisiers TT closers | | | | | | Ya S T RXFO 1961. -
Online Proofing
elsevier_BBABIO_47641 Emerging role of Lon protease as a master regulator of mitochondrial functions Marcello Pintia, 1 Lara Gibellinib, 1 Milena Nasib Sara De Biasib Carlo Augusto Bortolottia Anna Iannonec Andrea Cossarizzab, ⁎ [email protected] aDepartment of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy bDepartment of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy cDepartment of Diagnostics, Clinical and Public Health Medicine, University of Modena and Reggio Emilia, Modena, Italy ⁎Corresponding author at: Department of Surgery, Medicine, Dentistry, and Morphological Sciences, University of Modena and Reggio Emilia, Via Campi, 287, 41125 Modena, Italy. 1Equally contributed to the paper. This article is part of a Special Issue entitled '‘EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2‐–6, 2016'’, edited by Prof. Paolo Bernardi. Abstract Lon protease is a nuclear-encoded, mitochondrial ATP-dependent protease highly conserved throughout the evolution, crucial for the maintenance of mitochondrial homeostasis. Lon acts as a chaperone of misfolded proteins, and is necessary for maintaining mitochondrial DNA. The impairment of these functions has a deep impact on mitochondrial functionality and morphology. An altered expression of Lon leads to a profound reprogramming of cell metabolism, with a switch from respiration to glycolysis, which is often observed in cancer cells. Mutations of Lon, which likely impair its chaperone properties, are at the basis of a genetic inherited disease named of the cerebral, ocular, dental, auricular, skeletal (CODAS) syndrome. This article is part of a Special Issue entitled '‘EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2‐–6, 2016'’, edited by Prof. -
Gpu-Accelerated Applications
GPU-ACCELERATED APPLICATIONS Test Drive the World’s Fastest Accelerator – Free! Take the GPU Test Drive, a free and easy way to experience accelerated computing on GPUs. You can run your own application or try one of the preloaded ones, all running on a remote cluster. Try it today. www.nvidia.com/gputestdrive GPU-ACCELERATED APPLICATIONS Accelerated computing has revolutionized a broad range of industries with over five hundred applications optimized for GPUs to help you accelerate your work. CONTENTS 1 Computational Finance 2 Climate, Weather and Ocean Modeling 2 Data Science and Analytics 4 Deep Learning and Machine Learning 7 Federal, Defense and Intelligence 8 Manufacturing/AEC: CAD and CAE COMPUTATIONAL FLUID DYNAMICS COMPUTATIONAL STRUCTURAL MECHANICS DESIGN AND VISUALIZATION ELECTRONIC DESIGN AUTOMATION 15 Media and Entertainment ANIMATION, MODELING AND RENDERING COLOR CORRECTION AND GRAIN MANAGEMENT COMPOSITING, FINISHING AND EFFECTS EDITING ENCODING AND DIGITAL DISTRIBUTION ON-AIR GRAPHICS ON-SET, REVIEW AND STEREO TOOLS WEATHER GRAPHICS 22 Medical Imaging 22 Oil and Gas 23 Research: Higher Education and Supercomputing COMPUTATIONAL CHEMISTRY AND BIOLOGY NUMERICAL ANALYTICS PHYSICS SCIENTIFIC VISUALIZATION 33 Safety & Security 35 Tools and Management Computational Finance APPLICATION NAME COMPANY/DEVELOPER PRODUCT DESCRIPTION SUPPORTED FEATURES GPU SCALING Accelerated Elsen Secure, accessible, and accelerated back- * Web-like API with Native bindings for Multi-GPU Computing Engine testing, scenario analysis, risk analytics Python, R, Scala, C Single Node and real-time trading designed for easy * Custom models and data streams are integration and rapid development. easy to add Adaptiv Analytics SunGard A flexible and extensible engine for fast * Existing models code in C# supported Multi-GPU calculations of a wide variety of pricing transparently, with minimal code Single Node and risk measures on a broad range of changes asset classes and derivatives. -
UCLA Electronic Theses and Dissertations
UCLA UCLA Electronic Theses and Dissertations Title The Second Line: A (Re)Conceptualization of the New Orleans Brass Band Tradition Permalink https://escholarship.org/uc/item/09z2z2d7 Author GASPARD BOLIN, MARC TIMOTHY Publication Date 2021 Peer reviewed|Thesis/dissertation eScholarship.org Powered by the California Digital Library University of California UNIVERSITY OF CALIFORNIA Los Angeles The Second Line: A (Re)Conceptualization of the New Orleans Brass Band Tradition A dissertation submitted in partial satisfaction of the requirements for the degree Doctor of Philosophy in Ethnomusicology by Marc Timothy Gaspard Bolin 2021 i © Copyright by Marc Timothy Gaspard Bolin 2021 i ABSTRACT OF THE DISSERTATION The Second Line: A (Re)Conceptualization of the New Orleans Brass Band Tradition by Marc Timothy Gaspard Bolin Doctor of Philosophy in Ethnomusicology University of California, Los Angeles, 2021 Professor Cheryl L. Keyes, Chair In New Orleans, Louisiana, nearly every occasion is marked with a celebratory parade, most famously the Mardi Gras processions that seemingly take over the city during Carnival Time. But throughout the year, there are jazz funerals and parades known as "second lines" that fill the Backatown neighborhoods of New Orleans, with the jubilant sounds of brass band music. These peripatetic parades and their accompanying brass bands have become symbolic of New Orleans and its association with social norm-breaking and hedonistic behavior. The second line constitutes cultural practice and group identification for practitioners serving as a site for spiritual practice and renewal. In Los Angeles, California, practitioners are transposing the second line, out of which comes new modes of expression, identities, meanings, and theology. -
3D Graphics for Virtual Desktops Smackdown
3D Graphics for Virtual Desktops Smackdown 3D Graphics for Virtual Desktops Smackdown Author(s): Shawn Bass, Benny Tritsch and Ruben Spruijt Version: 1.11 Date: May 2014 Page i CONTENTS 1. Introduction ........................................................................ 1 1.1 Objectives .......................................................................... 1 1.2 Intended Audience .............................................................. 1 1.3 Vendor Involvement ............................................................ 2 1.4 Feedback ............................................................................ 2 1.5 Contact .............................................................................. 2 2. About ................................................................................. 4 2.1 About PQR .......................................................................... 4 2.2 Acknowledgements ............................................................. 4 3. Team Remoting Graphics Experts - TeamRGE ....................... 6 4. Quotes ............................................................................... 7 5. Tomorrow’s Workspace ....................................................... 9 5.1 Vendor Matrix, who delivers what ...................................... 18 6. Desktop Virtualization 101 ................................................. 24 6.1 Server Hosted Desktop Virtualization directions ................... 24 6.2 VDcry?! ........................................................................... -
Analisys of Long-Term Gravity Records in Europe: Consequences for The
UNIVERSIDAD COMPLUTENSE DE MADRID FACULTAD DE CIENCIAS MATEMÁTICAS SECCIÓN DEPARTAMENTAL DE ASTRONOMÍA Y GEODESÍA TESIS DOCTORAL Analisys of long-term gravity records in Europe: consequences for the retrieval of the small amplitude and low frecuency signals including the earth's core resonance effects Análisis de serie de datos de gravedad de larga duración en Europa: consecuencias para el estudio de señales de pequeña amplitud y baja frecuencia incluyendo los efectos de resonancia del núcleo terrestre MEMORIA PARA OPTAR AL GRADO DE DOCTORA PRESENTADA POR Marta Calvo García-Maroto Directores José Arnoso Sampedro Jacques Hinderer Madrid, 2015 ©Marta Calvo García-Maroto, 2015 UNIVERSIDAD COMPLUTENSE DE MADRID FACULTAD DE CIENCIAS MATEMÁTICAS SECCIÓN DEPARTAMENTAL DE ASTRONOMÍA Y GEODESIA UNIVERSITÉ DE STRASBOURG ECOLE DOCTORALE DES SCIENCES DE LA TERRE, DE L’UNIVERS ET DE L’ENVIRONNEMENT INSTITUT DE PHYSIQUE DU GLOBE DE STRASBOURG ANALYSIS OF LONG ---TERM GRAVITY RECORDS IN EUROPE; CONSEQUENCES FOR THE RETRIEVAL OF SMALL AMPLITUDE AND LOW FREQUENCY SIGNALS INCLUDING THE EARTH’S CORE RESONANCE EFFECTS ANÁLISIS DE SERIES DE DATOS DE GRAVEDAD DE LARGA DURACIÓN EN EUROPA; CONSECUENCIAS PARA EL ESTUDIO DE SEÑALES DE PEQUEÑA AMPLITUD Y BAJA FRECUENCIA, INCLUYENDO LOS EFECTOS DE RESONANCIA DEL NÚCLEO TERRESTRE MEMORIA PARA OPTAR AL GRADO DE DOCTOR PRESENTADA POR Marta Calvo GarcíaGarcía----MarotoMarotoMarotoMaroto Bajo la dirección de los Doctores: José Arnoso SampedroSampedroSampedro Científico Titular - Instituto de Geociencias (CSIC-UCM) Jacques -
Sarah Owdah Alomrani.Pdf
Expression of the rice cystatin, Oryzacystatin-I (OC-I) influences the plant growth and development Sarah Owdah Alomrani Submitted in accordance with the requirements for the degree of Doctor of Philosophy The University of Leeds Faculty of Biological Sciences Centre for Plant Sciences September 2020 i The candidate confirms that the work submitted is his/her own and that appropriate credit has been given where reference has been made to the work of others. This copy has been supplied on the understanding that it is copyright material and that no quotation from the thesis may be published without proper acknowledgement. © 2020, The University of Leeds, Sarah Alomrani ii Acknowledgment Today, I have completed the writing of my thesis during the lockdown period which has been enforced due to the COVID-19 pandemic. Before I thank those who have support me through my journey, I would like to mention that, when I got divorced, around five years ago, I felt broken and I will always remember that my father told me: “You can start on your next chapter as an independent Saudi women”. I thought that the only thing that people will know about me was that I had been divorced, but now that is not the case. I have worked hard to obtain a scholarship to study in UK in my favourite subject, plant sciences, and the most important thing about me now is that I’m a strong women who no longer hopes for someone who would bring flowers for her, but I am able to plant my own flowers. -
(12) United States Patent (10) Patent No.: US 9,353,359 B2 Sorek Et Al
US009353359B2 (12) United States Patent (10) Patent No.: US 9,353,359 B2 Sorek et al. (45) Date of Patent: May 31, 2016 (54) BACTERIAL ANTI-PHAGE DEFENSE OTHER PUBLICATIONS SYSTEMS Copeland et al. 2007; Complete sequence of Pseudomonus (71) Applicant: Yeda Research and Development Co. mendocinaymp. EMBL: ABP83335.* Ltd., Rehovot (IL) Lucas et al. 2008; Complete sequence of Acidithiobacillus fer rooxidans ATCC 53993. EMBL: ACH83539.1. (72) Inventors: Rotem Sorek, Rehovot (IL); Hila Kerfeld et al. 2009; Complete sequence of plasmid of Amonifex Sberro, Rehovot (IL): Azita Leavitt, degensii KC4, EMBL: ACX53253.1.* Rehovot (IL) Lee et al. 2006; Sequence analysis of two cryptic plasmids from Bifidobacterium longump.O10A and construction of a shuttle clon (73) Assignee: Yeda Research and Development Co. ing vector. Applied Environmental Microbiology 72:527-535. Ltd., Rehovot (IL) EMBL: ACD97784.1. Alignment Only Being Sent.* Chenoll et al. 2012; Safety assessment of strain Bifidobacterium (*) Notice: Subject to any disclaimer, the term of this longum CECT 7347, a probiotic able to reduce the toxicity and patent is extended or adjusted under 35 inflammatory potential of gliadin-derived peptides, based on pheno U.S.C. 154(b) by 0 days. typic traits, whole genome sequencing, and murine trials. EMBL: CCK35109.18 (21) Appl. No.: 14/140,793 PajonEMBL et al. Clife2010; The genome sequenceC of Bifidobacterium longumgu Schell et al. 2002; The genome sequence of Bifidobacterium longum (22) Filed: Dec. 26, 2013 reflects its Apato the R gastrointestinal tract. SS (65) Prior Publication Data 12-1427 EMBL: AAN25255.1. Alignment Only Being US 2014/O178353 A1 Jun. -
Emerging Role of Lon Protease As a Master Regulator of Mitochondrial Functions☆
Biochimica et Biophysica Acta 1857 (2016) 1300–1306 Contents lists available at ScienceDirect Biochimica et Biophysica Acta journal homepage: www.elsevier.com/locate/bbabio Emerging role of Lon protease as a master regulator of mitochondrial functions☆ Marcello Pinti a,1, Lara Gibellini b,1,MilenaNasib,SaraDeBiasib,CarloAugustoBortolottia, Anna Iannone c, Andrea Cossarizza b,⁎ a Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy b Department of Surgery, Medicine, Dentistry and Morphological Sciences, University of Modena and Reggio Emilia, Modena, Italy c Department of Diagnostics, Clinical and Public Health Medicine, University of Modena and Reggio Emilia, Modena, Italy article info abstract Article history: Lon protease is a nuclear-encoded, mitochondrial ATP-dependent protease highly conserved throughout the evo- Received 13 January 2016 lution, crucial for the maintenance of mitochondrial homeostasis. Lon acts as a chaperone of misfolded proteins, Received in revised form 23 March 2016 and is necessary for maintaining mitochondrial DNA. The impairment of these functions has a deep impact on mi- Accepted 24 March 2016 tochondrial functionality and morphology. An altered expression of Lon leads to a profound reprogramming of Available online 28 March 2016 cell metabolism, with a switch from respiration to glycolysis, which is often observed in cancer cells. Mutations of Lon, which likely impair its chaperone properties, are at the basis of a genetic inherited disease named of Keywords: Lon the cerebral, ocular, dental, auricular, skeletal (CODAS) syndrome. This article is part of a Special Issue entitled Protease ‘EBEC 2016: 19th European Bioenergetics Conference, Riva del Garda, Italy, July 2–6, 2016’,editedbyProf. -
Searches for Life and Intelligence Beyond Earth
Technologies of Perception: Searches for Life and Intelligence Beyond Earth by Claire Isabel Webb Bachelor of Arts, cum laude Vassar College, 2010 Submitted to the Program in Science, Technology and Society in Partial Fulfillment of the Requirements for the Degree of Doctor of Philosophy in History, Anthropology, and Science, Technology and Society at the Massachusetts Institute of Technology September 2020 © 2020 Claire Isabel Webb. All Rights Reserved. The author hereby grants to MIT permission to reproduce and distribute publicly paper and electronic copies of this thesis document in whole or in part in any medium now known or hereafter created. Signature of Author: _____________________________________________________________ History, Anthropology, and Science, Technology and Society August 24, 2020 Certified by: ___________________________________________________________________ David Kaiser Germeshausen Professor of the History of Science (STS) Professor of Physics Thesis Supervisor Certified by: ___________________________________________________________________ Stefan Helmreich Elting E. Morison Professor of Anthropology Thesis Committee Member Certified by: ___________________________________________________________________ Sally Haslanger Ford Professor of Philosophy and Women’s and Gender Studies Thesis Committee Member Accepted by: ___________________________________________________________________ Graham Jones Associate Professor of Anthropology Director of Graduate Studies, History, Anthropology, and STS Accepted by: ___________________________________________________________________ -
3D Computer Graphics
3D computer graphics Viktor Novičenko Smithy of ideas 2011 Main idea – I define for computer objects forms, colors, materials define lights source and it position. And computer will calculate how the objects is look like, looking from camera. This picture is “drawing” by computer! 2D computer graphics Primitive element - pixel features: color – defined by integer number from 0 to 255 red green There are another color palettes: HSV, CMYK blue 3D computer graphics Primitive element – polygon (restricted plane somehow oriented in virtual 3d space) Each object form can be constructed from polygons. Example screw: Computer per 7 min. 38 sec. will calculate how screw is look like using some kind of environment. workflow: • modeling – object form construction from polygons • texturing, shading – define object material and accept colors, using 2d picture (texture) • animation (if we want make movie) – define movement of polygons in scene • rendering – final picture calculation And after render we have .... Plenty of tools in 3d packages: Photoshop – 2d graphic redactor Maya – 3d graphic redactor Texturing – we must make UV layout to define each polygons pixel’s color. Material definition (shading) – a lot of parameters, which influence of reflection and refraction algorithms. Most heavy materials is “subsurface scaterring” Materials. Render – program which on input has scene file(polygonal objects, materials, textures, lights sources and camera position) and on output is final 2d picture. bias unbias Indigo render Vray (bulgarija) Maxwell render Mental ray (vokietija) Octane render Renderman (JAV) 5 years ago coputers is to slow for using Final-Render (D.Britanija, Kanada) unbias rendering algorithms. Bias Unbias Light source Light source Camera Camera Object surface Object surface Bias renders has direct and indirect Unbias renders is closed to real world.