Orthopaedic Bioengineering Research Laboratory 2008-2009
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2008-2009 Report including the Orthopaedic Bioengineering Research Laboratory Dedication This issue of achievement at the Orthopaedic Research Center and Ortho- paedic Bioengineering Research Laboratory at Colorado State University is dedicated to Alec Wildenstein who lost a long battle with prostate cancer and passed away in 2008. Alec was a well known racehorse owner and breeder and a member of the Orthopaedic Research Center’s Advisory Board. Ecurie Wildenstein won the Prix de l’Arc de Triomphe four times with the most re- cent being won by Peintre Celebre who also gave the family their first French Derby victory. Their racing successes stretched into the United States, and Arcangues is best known for coming over and winning the Breeders’ Cup Classic. Dr. McIlwraith has consulted and done surgery for the Wildenstein family for over 20 years. Alec supported research projects at the ORC par- ticularly the global project on conformation in Thoroughbreds documenting the changes that occur in growth as well as the effect of different conforma- tions on equine soundness. Preface It is my pleasure to present our 2008-2009 Report infusion to increase the number of horses that we from the Orthopaedic Research Center and the could evaluate; the K08 training grant of Dr. Laurie Orthopaedic Bioengineering Research Laboratory Goodrich received an additional grant of slightly over at Colorado State University. Our principal focus $100,000 to do a dose titration for gene therapy and continues to be solving the significant problems in a new grant involving stem cell therapy for cartilage equine musculoskeletal disease as can be seen in this healing headed up by Dr. Connie Chu at the Univer- report, but we will also continue to investigate ques- sity of Pittsburgh with Drs. Goodrich, McIlwraith and tions relative to human joint disease and techniques Kisiday involved in an equine subcontract was also and devices for human osteoarthritis and articular recently funded. cartilage repair when the technique can also benefit the horse. We continue to partner with the labora- We added a fourth building to our Orthopaedic tories of Dr. Alan Grodzinsky at MIT on an NIH Research Center complex, namely a Gait Center Program Grant in cartilage repair (Dr. Frisbie PI of where gait analysis (kinetics and kinematics) for sub-contract) and also with Dr. Robert Sah of UC San both horses and dogs has been set up. Much of the Diego on another NIH Grant in joint lubrication and equipment for gait analysis came from courtesy of osteoarthritis (Dr. McIlwraith collaborator) and more Dr. Robert Taylor and the Thaw Family Foundation recently with Dr. Jude Samulski at the University of and a 3 year scholarship for a Ph.D. student in canine North Carolina with Dr. Laurie Goodrich’s NIH K08 rehabilitation was also provided by Jaynn and Walter grant on gene therapy (co-mentored by Drs. Samulski Emery. and McIlwraith). Accomplishments at the ORC over the past 2 years It is probably safe to say that all research institutions are detailed in this report. These accomplishments at universities have had the same challenges we’ve could not be achieved without our team of faculty had in 2008-2009 because of the economic recession. and staff as well as the excellent support of our corpo- Our endowed corpuses which provide the salaries rate and individual donors and funding from research for our four endowed chairs within the Orthopaedic agencies. With this help, we continue to achieve our Research Center have certainly suffered greatly in goals and also make new ones as new clinical ques- the last 18 months. Fortunately we have had enough tions arise. donated money uncommitted to endowments to make up for salary needs and we have continued to Best wishes, receive good external research funding which is based on the ability of our faculty and staff to compete for these dollars. We have received three “top-ups” from NIH with stimulus package grants. The program grant from MIT that Dave Frisbie is PI on the sub- contract for cartilage healing received an additional Wayne McIlwraith 2 Table of Contents Dedication ........................................................................1 Research Goals ..........................................................54 Research Goals Achieved 2008-2009 .............54 Preface ...............................................................................2 Research Goals for the Future and Mission ..............................................................................5 Current Research .........................................56 Research Focuses of the Orthopaedic Research Techniques Available at the Research Center ..............................................................7 Orthopaedic Research Center ....................................59 Research Focuses at the Orthopaedic Research Techniques Available at the Bioengineering Research Laboratory .........................8 Orthopaedic Bioengineering Research Musculoskeletal Research Program............................9 Laboratory ......................................................................61 School of Biomedical Engineering............................10 Scientific Publications and Presentations ...............62 Faculty .............................................................................11 Funded Research Projects ...........................................89 College of Veterinary Medicine and Biomedical Revenue and Expense, FY08 to FY09 .......................94 Sciences .................................................................11 Honors and Awards ......................................................97 College of Engineering ............................................20 College of Applied Human Sciences ......................22 Editorial and Scientific Advisory Boards College of Agricultural Sciences .............................22 2008-2009 .......................................................................98 College of Natural Sciences .....................................23 Professional Associations 2008-2009 .......................99 Affiliate Faculty .........................................................24 Collaborators .............................................................26 Advisory Board ...........................................................101 2008-2009 Post Doctoral Fellows ...........................33 Our Donors ..................................................................102 2008-2009 Ph.D. Graduate Students ......................34 2008-2009 D.V.M./Ph.D. Graduate Students ........37 Summary of Research Projects 2008-2009 ............107 2008-2009 M.S. Graduate Students ........................38 Focus 1. Musculoskeletal Tissue Healing Research Associates ..................................................41 Induction of bone marrow mesenchymal stem Staff Veterinarian ......................................................44 cell chondrogenesis following short-term Administrative Staff ..................................................44 suspension culture ............................................108 Areas of Expertise of Personnel .................................45 Clinical follow-up of horses treated with bone marrow derived mesenchymal stem cells for Student Work Study/Student Hourly musculoskeletal lesions ....................................111 Assistants at ORC 2008-2009 .....................................46 Osteochondral allografts for use in equine Volunteers at ORC ........................................................46 cartilage resurfacing ..........................................113 Autologous and commercially derived fibrin Graduate Students – Placement Since Inception ...47 glues as a delivery vehicle for mesenchymal Surgery Residents Supervised (and Outcome) ......50 stem cells ............................................................114 Self-complementary adeno-associated viral Program Synopsis .........................................................51 vectors exhibit high efficiency in joint History ........................................................................51 tissues depending on serotype selection ........116 Research Activities ...................................................51 Impact ........................................................................52 Program Trends ........................................................52 Program Goals ..........................................................53 Goals Accomplished 2008-2009 ......................53 Current Goals ....................................................53 3 Table of Contents Continued Osteoblastic differentiation of human Focus 4. Continued Development of Novel and equine bone marrow-derived Therapies for Traumatic Synovitis, Capsulitis mesenchymal stem cells with combined and Osteoarthritisin the Horse bone morphogenetic protein 2 and 7 genetic Evaluation of intraarticular polysulfated modification in the presence and absence of glycosaminoglycan or sodium hyaluronan dexamethasone ..................................................119 for treatment of osteoarthritis using an Lag screw fixation of dorsal cortical stress equine experimental model .............................144 fractures in 116 racehorses ..............................122 Evaluation of topical 1% diclofenac for treatment of equine osteoarthritis using an Focus 2. Early Diagnosis of Bone and Joint Disease equine experimental model .............................148 A new technique for examination of the Evaluation of extracorporeal