Orthopaedic Bioengineering Research Laboratory 2010-2011
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2010-2011 Report including the Orthopaedic Bioengineering Research Laboratory Preface It is my pleasure to present our 2010-2011 report been possible without a number of our donors from the Orthopaedic Research Center and the stepping up to provide supplemental funding. Orthopaedic Bioengineering Research Laboratory I’m particularly grateful to Mr. Jim Kennedy for at Colorado State University. Our principal focus providing supplemental operating funds and continues to be solving the significant problems in continuing the legacy of his mother, Barbara Cox equine musculoskeletal disease as can be seen in this Anthony. I’m also grateful to Abigail Kawananakoa report but we also continue to investigate questions for continued support over and above the Endowed relevant to human joint disease and techniques Chair she donated three years ago; and Herbert Allen and devices for human osteoarthritis and articular for continuing to provide considerable support for cartilage repair when the technique can also benefit investigation of cutting edge therapies. the horse. There have been a number of notable projects in this regard. The studies, led by Dr. Dave We have added an Equine Sports Medicine Frisbie at the ORC in partnership with Dr. Alan Ambulatory clinical arm to the Orthopaedic Research Grodzinsky at MIT on an NIH Program Grant in Center and also initiated two residencies in Sports cartilage repair, have been completed and results Medicine and Rehabilitation. This follows on from are still be analyzed. We have three other ongoing the accreditation of a new specialty college, the NIH grants in partnership with collaborators. One is American College of Veterinary Sports Medicine and with Dr. Connie Chu at the University of Pittsburgh Rehabilitation. Drs. Haussler, Frisbie and Kawcak, as looking at stem cells in fibrin/PRP and articular well as myself, are Foundation Diplomates and felt cartilage repair (Dr. Laurie Goodrich is the PI on the we had the appropriate ability and responsibility to sub-contract at CSU and other collaborators include train residents in this new specialty. Dr. Dora Ferris Dr. Lisa Fortier of Cornell University and Dr. Bob Sah is in her second year and Dr. Erin Contino is in her of the University of California, San Diego). This was a first year. I would like to extend special thanks to 12-month study that has been completed and results Gail Holmes for providing funding for the three year are still being analyzed with the other collaborators. program for Erin Contino. The work with Dr. Jude Samulski at the University of North Carolina on Dr. Laurie Goodrich’s NIH KO8 Accomplishments at the ORC over the past two grant on gene therapy (co-mentored by Drs. Samulski years are detailed in this report. Validation and and McIlwraith) is progressing well and we have clarification of the benefits of mesenchymal stem some exciting results with regard to long term protein therapy, platelet-rich plasma (PRP), as well as production with the AAV vector. The third NIH grant autologous conditioned serum (IRAP II) are all is with Dr. Steve Trippel at the University of Indiana in this report. These accomplishments could not in which Drs. Frisbie and McIlwraith are co-PIs on a be achieved without our team of faculty and staff sub-contract of an NIH grant involving gene therapy as well as the excellent support of equine funding and articular cartilage repair and the equine study agencies (Grayson-Jockey Club Research Foundation, will commence in the summer of 2012. American Quarter Horse Association, and United States Equestrian Federation), corporate funding, There have been some significant publications and individual donors. With this help we continue from the horse-human collaborative projects in to achieve our goals and also make new ones as new leading journals in the last two years. At the same clinical questions arise. time, many projects addressing equine specific problems have been completed and published and Best wishes, all of this is detailed in this report. We handled the challenges created by the economic recession well in the 2010-2011 period. This would not have Wayne McIlwraith 1 Table of Contents Preface ...............................................................................1 Program Goals ..........................................................49 Goals Accomplished 2010-2011 ......................49 Mission ..............................................................................4 Current Goals ....................................................50 Research Focuses of the Orthopaedic Research Goals ..........................................................50 Research Center ..............................................................6 Research Goals Achieved 2010-2011 .............50 Research Focuses at the Orthopaedic Future and Current Research at the ORC .............54 Bioengineering Research Laboratory .........................7 Research Techniques Available at the Musculoskeletal Research Program............................9 Orthopaedic Research Center ....................................57 School of Biomedical Engineering............................10 Research Techniques Available at the Orthopaedic Bioengineering Faculty .............................................................................11 Research Laboratory ....................................................59 College of Veterinary Medicine and Biomedical Sciences ............................................11 Scientific Publications and Presentations ...............60 College of Engineering ............................................20 Funded Research Projects ...........................................87 College of Applied Human Sciences ......................22 Revenue and Expense, FY10 to FY11 .......................92 College of Agricultural Sciences .............................22 College of Natural Sciences .....................................23 Honors and Awards ......................................................95 Affiliate Faculty .........................................................24 Editorial and Scientific Advisory Boards Collaborators .............................................................26 2010-2011 .......................................................................96 2010-2011 Post Doctoral Fellows ...........................33 2010-2011 Equine Sports Professional Associations 2010-2011 .......................97 Medicine Residencies ........................................34 Advisory Board 2011 ...................................................99 2010-2011 Ph.D. Graduate Students ......................35 2010-2011 M.S. Graduate Students ........................37 Our Donors ..................................................................100 Research Scientist .....................................................38 Summary of Research Projects 2010-2011 ............105 Research Associates ..................................................38 Research Coordinator ..............................................41 Focus 1. Musculoskeletal Tissue Healing Administrative Staff ..................................................41 Consensus on Equine Tendon Disease: Director Gait Analysis Center/ Building on the 2007 Havemeyer ORC Staff Veterinarian .......................................41 Symposium ........................................................106 Equine Bone Marrow-Derived Stem Cells: Orthopaedic Research Program ................................42 Comparing the Ilium and the Sternum .........109 Areas of Expertise of Personnel ..............................42 Equine Mesenchymal Stem Cells in Vitro Student Hourly Assistants at the ORC 2010-2011 .43 Differentiation Capacity: Differences Between Sternum and Ilium ............................111 Volunteers at the ORC 2010-2011 .............................43 Equine Models of Articular Cartilage Repair .....113 Graduate Students – Placement Since Inception ...44 Evaluation of Intra-Articular Mesenchymal Stem Cells to Augment Healing of Surgery Residents Supervised (and Outcome) ......46 Microfractured Chondral Defects ..................116 Program Synopsis .........................................................47 Expansion of Adult Equine Bone Marrow History ........................................................................47 Mesenchymal Stem Cells on Fibrinogen- Research Activities ...................................................47 Rich Protein Surfaces Derived From Impact ........................................................................48 Blood Plasma .....................................................119 Program Trends ........................................................48 2 Table of Contents Continued Focus 2. Early Diagnosis of Bone and Joint Disease Focus 4. Continued Development of Novel Incidence of Radiographic Changes in Young Therapies for Traumatic Synovitis, Capsulitis, Cutting Horses ...................................................122 and Osteoarthritis in the Horse A Descriptive Study of the Equine Proximal Effects of Platelet-Rich Plasma Composition on Interphalangeal Joint Using Magnetic Anabolic and Catabolic Activities in Equine Resonance Imaging, Contrast Arthrography, Cartilage and Meniscal Explants.....................153 and Arthroscopy ...............................................125 Gene Therapy Approach to Treat Osteoarthritis The Relationship Between Radiographic Using AAVIRAP................................................157 Changes and Performance Outcomes in Influences