Picrosirius Red and Masson's Trichrome Staining
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Orthopaedic Bioengineering Research Laboratory 2010-2011
2010-2011 Report including the Orthopaedic Bioengineering Research Laboratory Preface It is my pleasure to present our 2010-2011 report been possible without a number of our donors from the Orthopaedic Research Center and the stepping up to provide supplemental funding. Orthopaedic Bioengineering Research Laboratory I’m particularly grateful to Mr. Jim Kennedy for at Colorado State University. Our principal focus providing supplemental operating funds and continues to be solving the significant problems in continuing the legacy of his mother, Barbara Cox equine musculoskeletal disease as can be seen in this Anthony. I’m also grateful to Abigail Kawananakoa report but we also continue to investigate questions for continued support over and above the Endowed relevant to human joint disease and techniques Chair she donated three years ago; and Herbert Allen and devices for human osteoarthritis and articular for continuing to provide considerable support for cartilage repair when the technique can also benefit investigation of cutting edge therapies. the horse. There have been a number of notable projects in this regard. The studies, led by Dr. Dave We have added an Equine Sports Medicine Frisbie at the ORC in partnership with Dr. Alan Ambulatory clinical arm to the Orthopaedic Research Grodzinsky at MIT on an NIH Program Grant in Center and also initiated two residencies in Sports cartilage repair, have been completed and results Medicine and Rehabilitation. This follows on from are still be analyzed. We have three other ongoing the accreditation of a new specialty college, the NIH grants in partnership with collaborators. One is American College of Veterinary Sports Medicine and with Dr. -
Altmetric.Com and Plumx
This is a preprint of an article published in Scientometrics. The final authenticated version is available online at: https://doi.org/10.1007/s11192-021-03941-y A large-scale comparison of coverage and mentions captured by the two altmetric aggregators- Altmetric.com and PlumX Mousumi Karmakara, Sumit Kumar Banshalb, Vivek Kumar Singha,1 1Department of Computer Science, Banaras Hindu University, Varanasi-221005, India 2Department of Computer Science, South Asian University, New Delhi-110021, India. Abstract: The increased social media attention to scholarly articles has resulted in creation of platforms & services to track the social media transactions around them. Altmetric.com and PlumX are two such popular altmetric aggregators. Scholarly articles get mentions in different social platforms (such as Twitter, Blog, Facebook) and academic social networks (such as Mendeley, Academia and ResearchGate). The aggregators track activity and events in social media and academic social networks and provide the coverage and transaction data to researchers for various purposes. Some previous studies have compared different altmetric aggregators and found differences in the coverage and mentions captured by them. This paper attempts to revisit the question by doing a large-scale analysis of altmetric mentions captured by the two aggregators, for a set 1,785,149 publication records from Web of Science. Results obtained show that PlumX tracks more altmetric sources and captures altmetric events for a larger number of articles as compared to Altmetric.com. However, the coverage and average mentions of the two aggregators, for the same set of articles, vary across different platforms, with Altmetric.com recording higher mentions in Twitter and Blog, and PlumX recording higher mentions in Facebook and Mendeley. -
FUCHSIN ACID Powder Dye, C.I. 42685
FUCHSIN ACID powder dye, C.I. 42685 IVD In vitro diagnostic medical device Acid Fuchsine, Acid Violet 19, BSC certified dye For staining of connective tissues using Van Gieson and Mallory methods INSTRUCTIONS FOR USE REF Catalogue number: FA-P-25 (25 g) Introduction Histology, cytology and other related scientific disciplines study the microscopic anatomy of tissues and cells. In order to achieve a good tissue and cellular structure, the samples need to be stained in a correct manner. Fuchsin Acid is a triarylmethane dye that can be used for trichrome staining of connective tissues according to Mallory and Van Gieson. Mallory developed staining method for visualizing collagen connective tissues, modified and advanced during time. If the sample is fixated in Zenker's solution, it enables quality separation of individual tissue components. The Van Gieson dye solution is used as a contrasting dye in the mentioned method. Picric acid in the solution is the source of acid pH value and functions as cytoplasmic and muscle dye. Product description FUCHSIN ACID - Biological Stain Commision (BSC) certified powder dye for creating solution for connective tissues staining according to Van Gieson and Mallory methods. Other preparations and reagents used in preparing the dye solution: Phosphotungstic acid (H3PW12O40·xH2O) Picric acid (C6H3N3O7) Microscopy powder dyes, such as BioGnost's Orange G dye (product code OG-P-25, OG-P-100) Microscopy powder dyes, such as BioGnost's Aniline Blue dye (product code CAB-P-25G) Preparing the solutions for staining Mallory's dyes for connective tissues: 0.25% solution of Fuchsin Acid dye Dissolve 0.25 g of Fuchsin Acid dye in 100 ml of distilled/demineralized water. -
RELX Group Annual Reports and Financial Statements 2015
Annual Reports and Financial Statements 2015 Annual Reports and Financial Statements 2015 RELX Group is a world-leading provider of information and analytics for professional and business customers across industries. We help scientists make new discoveries, lawyers win cases, doctors save lives and insurance companies offer customers lower prices. We save taxpayers and consumers money by preventing fraud and help executives forge commercial relationships with their clients. In short, we enable our customers to make better decisions, get better results and be more productive. RELX PLC is a London listed holding company which owns 52.9 percent of RELX Group. RELX NV is an Amsterdam listed holding company which owns 47.1 percent of RELX Group. Forward-looking statements The Reports and Financial Statements 2015 contain forward-looking statements within the meaning of Section 27A of the US Securities Act of 1933, as amended, and Section 21E of the US Securities Exchange Act of 1934, as amended. These statements are subject to a number of risks and uncertainties that could cause actual results or outcomes to differ materially from those currently being anticipated. The terms “estimate”, “project”, “plan”, “intend”, “expect”, “should be”, “will be”, “believe”, “trends” and similar expressions identify forward-looking statements. Factors which may cause future outcomes to differ from those foreseen in forward-looking statements include, but are not limited to competitive factors in the industries in which the Group operates; demand for the Group’s products and services; exchange rate fluctuations; general economic and business conditions; legislative, fiscal, tax and regulatory developments and political risks; the availability of third-party content and data; breaches of our data security systems and interruptions in our information technology systems; changes in law and legal interpretations affecting the Group’s intellectual property rights and other risks referenced from time to time in the filings of the Group with the US Securities and Exchange Commission. -
Orthopaedics & Traumatology: Surgery & Research
Author guidelines Orthopaedics & Traumatology: Surgery & Research Revue de Chirurgie Orthopédique et Traumatologique Orthopaedics & Traumatology: Surgery & Research (OTSR) and its French version Revue de Chirurgie Orthopédique et Traumatologique (RCOT) publish original scientific works in English and French related to orthopaedics from all domains. All the original articles, systematic reviews, meta-analysis, review articles, technical notes, concise follow-up of a former OTSR study are published in English and French (OTSR-RCOT does not publish Case reports): in English (OTSR) in electronic form only and in French (RCOT) in paper and electronic editions. Only the English version (OTSR) is indexed in international databases. Original articles must not have been published elsewhere or be simultaneously submitted for publication in another journal. The journal agrees to use the “Uniform Requirements for manuscripts submitted to biomedical journals” (www.icjme.org). It also adheres to the rules developed by the Committee on Publication Ethics (COPE) and the recommendations of the French National Authority for Health (HAS). Authors must submit an electronic version only of the article using the journal’s online submission site: https://www.editorialmanager.com/otsr/. French-speaking authors should submit in French. Non-French-speaking authors can submit in either French or English. All articles accepted and submitted in French will be translated from French to English by the Editorial Board. Manuscripts submitted in English will not be translated into French. When the original article is submitted in English, the corresponding French version in RCOT contains only the title (in French and English), the abstract in French or English, and the reference needed to access the full-text article. -
Reagents for Hospitals Medical and Research Laboratories Reagents for Hospitals
Reagents for Hospitals Medical and Research Laboratories Reagents for Hospitals Summary About us 4 Medical Laboratories 6 Microscopy / p. 7 Indroduction / p. 7 Reagents for Histology / p. 30 Giemsa Stain Sample Processing / p. 8 PAS Staining Getting the sample Masson’s Trichrome Staining Types of processing Reticulin fiber staining Pathological anatomy laboratory process From sampling to processing Reagents for Cytology / p. 38 Papanicolaou Stain Techniques and Stages / p. 10 Fixing / p. 10 Reagents for Clinical Microbiology / p. 41 Types of Chemical Fixative Agents Gram Staining Formaldehyde Fixation Procedure Ziehl-Neelsen Stain Histofix pre-dosed and Substitutes of Formaldehyde Other staining solutions Reagents for Fixing Decalcifiers Reagents for Hematology / p. 48 Kit for Fast Staining in Haematology (Fast Panoptic) Drying and Clearing / p. 16 May Grünwald-Giemsa or Pappenheim Stain Reagents for Drying Wright’s Stain Reagents for Clearing Other Products Inclusion / p. 19 Auxiliary Products / p. 52 Embedding media General Reagents Reagents for Embedding (paraffins) pH Indicator strips Derquim detergents Cutting / p. 20 Rehydration / p. 21 Deparaffinization-Hydration Reagents for Deparaffinization-Hydration Staining / p. 22 Dyes for microscopy Hematoxylin-Eosin Stain Hematoxylins Eosins Reagents for Staining Powdered dyes Dyes in solution Mounting / p. 29 Mounting media and Immersion oils 2 Panreac Applichem Research Laboratories 58 Reagents for Genomics / p. 58 PCR / p. 58 DNA Decontamination / p. 59 Gel electrophoresis / p. 59 Nucleic Acid Isolation / p. 59 Cloning Assays / p. 60 Buffers and Solvents / p. 60 Reagents for Proteomics / p. 61 Products for electrophoresis and blotting / p. 61 Reagents for Cell Culture / p. 64 Banish cell culture contamination / p. 64 Trends on new techniques for Clinical Diagnosis 65 Liquid Biopsies / p. -
Pituitary Gland
Part 6: Pituitary Gland Normal Physiology and Structure The pituitary gland comprises the adenohypophysis, which is made up of the pars distalis, pars intermedia and pars tuberalis and the neurohypophysis which includes the pars nervosa, infundibular stem and median eminence. The pars distalis forms the largest proportion of the gland and functions as the overall regulator of peripheral endocrine function by synthesizing and secreting at least 6 major trophic hormones. These include growth hormone (GH), prolactin (PrL), adrenocorticotrophic hormone (ACTH), thyroid stimulating hormone (TSH), luteinizing hormone (LH) and follicle stimulating hormone (FSH). Since this is the important area of the pituitary with respect to detecting endocrine active compounds, the rest of this section will concentrate only on this part of the pituitary. For reviews see (Page, 1994; Tucker, 1999; Greaves, 2007). Each hormone of the pars distalis is generally secreted by a seperate cell type, but some cells are able to secrete two hormones. The different hormones impart different staining properties to the cells. Using histological stains based on Orange G and periodic acid-Schiff (PAS), the cells of the pars distalis have been divided into acidophils (orange G positive), basophils (PAS positive) and chromophobes (absence of staining). In the rat, these have been reported to constitute 40, 10 and 50% respectively of the cell population of the pars distalis. The staining characteristics are dependent on the level of secretory activity, and when the cells have just secreted their granules or when secretory activity is increased, all the cells take on chromophobic characteristics due to the relative abundance of secretory organelles (endoplasmic reticulum and Golgi) and relative lack of secretory granules. -
New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues C
ORIGINAL PAPER New Tetrachromic VOF Stain (Type III-G.S) for Normal and Pathological Fish Tissues C. Sarasquete,* M. Gutiérrez Instituto de Ciencias Marinas de Andalucía, CSIC Polígono Río San Pedro, Apdo oficial, Puerto Real, Cádiz, Spain richrome methods invariably use dyes in acid ©2005, European Journal of Histochemistry pH solvents, usually diluted in aqueous acetic Tacid, and the concentration of this acid A new VOF Type III-G.S stain was applied to histological sec- matches the concentration of dye. Staining depends tions of different organs and tissues of healthy and pathologi- largely on the attachment of dyes to proteins. The cal larvae, juvenile and adult fish species (Solea senegalensis; acid pH itself is necessary to maximise the amount Sparus aurata; Diplodus sargo; Pagrus auriga; Argyrosomus regius and Halobatrachus didactylus). In comparison to the of dye that will attach to tissue amino groups. original Gutiérrez´VOF stain, more acid dyes of contrasting Proteins have both positively (amino groups) and colours and polychromatic/metachromatic properties were negatively (carboxyl and hydroxyl) charged groups. incorporated as essential constituents of the tetrachromic VOF Usually one predominates and this will have an stain. This facilitates the selective staining of different basic tissues and improves the morphological analysis of histo- overall negative or positive charge (being an acid or chemical approaches of the cell components. The VOF-Type III a basic protein). These charges can, however, bal- G.S stain is composed of a mixture of several dyes of varying ance each other out to some degree. Phosphate size and molecular weight (Orange G< acid Fuchsin< Light green<Methyl Blue<Fast Green), which are used simultane- groups of DNA and binding-proteins are important ously, and it enables the individual tissues to be selectively dif- in nuclear staining.The ionisation of basic groups of ferentiated and stained. -
Comparative Examination of Commonly Used Some Fixatives with Routine Histochemical Staining’S for the Optimal Histological Appearance in the Gill Tissue of Zebrafish
Kocatepe Veterinary Journal Kocatepe Vet J (2019) 12(2):158-167 RESEARCH ARTICLE DOI: 10.30607/kvj.526779 Comparative Examination of Commonly Used Some Fixatives with Routine Histochemical Staining’s for The Optimal Histological Appearance in The Gill Tissue of Zebrafish Aykut ULUCAN1*, Hayati YÜKSEL2, Muhammed Bahaeddin DÖRTBUDAK2, Seda YAKUT3 1Bingol University, Vocational School of Health Services, Department of Medical Services and Techniques, Turkey 2Bingol University, Faculty of Veterinary Medicine, Department of Pathology, Turkey 3Bingol University, Faculty of Veterinary Medicine, Department of Histology and Embryology, Turkey ABSTRACT Histopathological studies related to Zebrafish which are used as a model organism in researching the pathogenesis of many diseases increase day by day. Because of the increasing importance of zebrafish research, experiments on this animal model are more prominent and gill tissue is frequently examined in various disease models using zebrafish. As it is known, at the end of the experimental animal model studies, if the histopathological examination is to be done, the tissues should be fixed. The purpose of fixation is to keep cellular and extracellular components in vivo as much as possible. Therefore, the choice of fixation methods and fixatives has a significant effect on tissue processing. In this study, we aimed to optimize fixation techniques and staining protocols for producing ideal slides and histological images of gill tissue in zebrafish. In our study, to determine the optimal histology in the zebrafish-gill tissue, the tissues were fixed with Bouin’s, Carnoy’s, 10% neutral buffered formalin (NBF), Davidson’s, and Dietrich’s solutions. Following the routine tissue processing, the sections were stained with Hematoxylin and Eosin (H&E) and Masson’s Trichrome (MT) stains. -
The Mipim Awards 2016 Finalists
15 to 18 March 2016 - Palais des Festivals, Cannes Press release THE MIPIM AWARDS 2016 FINALISTS Paris, 28 January, 2016 - The eleven members of the international jury for the MIPIM Awards 2016 have released the list of the 44 finalists for this prestigious competition. Organised by Reed MIDEM, a subsidiary of Reed Exhibitions, MIPIM will take place in Cannes from March 15-18, 2016. This year, 230 projects from 42 countries have been submitted to one of the 11 categories of the MIPIM Awards. 2016 features a new category – ‘the Best Healthcare development' - which focusses on the fast-growing healthcare property segment. "The projects were carefully examined by the jury which represents the whole scope of the real estate industry. I found it particularly encouraging to see the growing number of projects in the residential category, including social housing and forward-thinking concepts. Also, the new category Best Healthcare Development is in tune with the need of healthcare facilities in an aging European society," said the President of the Jury, Barbara Knoflach, Deputy Chief Executive & Global Head of Investment Management at BNP Paribas Real Estate. The winners will be selected by both attendees voting in Cannes for projects of their choice and the jury. The jury’s decision will count for 50% of the vote as will the attendees’ share. For the fifth year in a row, the "People’s Choice Award” will reward the project that won the most votes online in the run-up to MIPIM. The public will soon be able to vote for this category on the MIPIM website and the show’s official Facebook page. -
The Histochemical Distribution of Placental Calcium and Alkaline Phosphatase Activity Following Fetoplacental Dissociation in Th
Loyola University Chicago Loyola eCommons Master's Theses Theses and Dissertations 1976 The Histochemical Distribution of Placental Calcium and Alkaline Phosphatase Activity Following Fetoplacental Dissociation in the Albino Rat eric sigmond Loyola University Chicago Follow this and additional works at: https://ecommons.luc.edu/luc_theses Part of the Medical Anatomy Commons Recommended Citation sigmond, eric, "The Histochemical Distribution of Placental Calcium and Alkaline Phosphatase Activity Following Fetoplacental Dissociation in the Albino Rat" (1976). Master's Theses. 2872. https://ecommons.luc.edu/luc_theses/2872 This Thesis is brought to you for free and open access by the Theses and Dissertations at Loyola eCommons. It has been accepted for inclusion in Master's Theses by an authorized administrator of Loyola eCommons. For more information, please contact [email protected]. This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License. Copyright © 1976 eric sigmond THE HISTOCHEMICAL DISTRIBUTION OF PLACENTAL CALCIUM AND ALKALINE PHOSPHATASE ACTIVITY FOLLOWING FETOPLACENTAL DISSOCIATION IN THE ALBINO RAT by Eric Sigmond A Thesis Submitted to the Faculty of the Graduate School of Loyola University of Chicago in Partial Fulfillment of the Requirements for the Degree of Master of Science February 1976 ACKNOWLEDGEMENT I wish to express my gratitude to Dr. Leslie A. Emmert for his suggestion of the problem, his patience, encouragement and supervision throughout the course of this thesis. His guidance helped overcome many problems which arose during the course of this study. I also wish to express thanks to Dr. Charles C.C. O'Morchoe and Dr. Maurice V. L'Heureux for their many valuable suggestions during the writing of this thesis. -
Tissue in Cases of Acute Rheumatism
Ann Rheum Dis: first published as 10.1136/ard.16.3.307 on 1 September 1957. Downloaded from Ann. rheum. Dis. (1957), 16, 307. HISTOCHEMICAL INVESTIGATION OF CONNECTIVE TISSUE IN CASES OF ACUTE RHEUMATISM BY A. I. STRUKOV AND G. V. ORLOVSKAYA U.S.S.R. Academy of Medical Sciences, Moscow (RECEIVED FOR PUBLICATION JULY 5, 1957) The connective tissue is the chief place where the phases: soluble (procollagen) and insoluble (colla- pathological rheumatic process takes place. The stromine). Obtained by precipitation from a changes mainly affect its intercellular components. solution, procollagen has all the properties of On the basis of this localization of the pathological collagen fibrils (of collagen): it turns red when it is lesions, rheumatism has been included among the stained by Van Gieson stain, and its roentgenogram so-called collagen diseases (Klemperer, 1950). The shows the characteristic interplane intervals of mechanism of development of the pathological 2-9 A and 11-0 A. Under the electron microscope by copyright. processes in the intercellular substance of connective it shows the same transverse bands. tissue can be understood only when there is a clear In a roentgenogram, collastromine shows no idea of the nature, structure, and correlations of ring at 2 9 A which is characteristic for collagen; collagen and the amorphous cementing substance, it has no periodicity, does not turn red with Van both of which play a principal role in the develop- Gieson stain, and shows a distinct metachromasia; ment of the rheumatic process. staining with silver reveals argyrophilic fibres. The The investigations made in recent years with the results of fractioning show collagen as at least a aid of physical, chemical, and histochemical bi-phasic system; the known features of collagen methods have considerably advanced our concepts in toto are determined by its content of procollagen.