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Assessing Neurotoxicity of Drugs of Abuse
National Institute on Drug Abuse RESEARCH MONOGRAPH SERIES Assessing Neurotoxicity of Drugs of Abuse 136 U.S. Department of Health and Human Services • Public Health Service • National Institutes of Health Assessing Neurotoxicity of Drugs of Abuse Editor: Lynda Erinoff, Ph.D. NIDA Research Monograph 136 1993 U.S. DEPARTMENT OF HEALTH AND HUMAN SERVICES Public Health Service National Institutes of Health National Institute on Drug Abuse 5600 Fishers Lane Rockville, MD 20857 ACKNOWLEDGMENT This monograph is based on the papers and discussions from a technical review on “Assessing Neurotoxicity of Drugs of Abuse” held on May 20-21, 1991, in Bethesda, MD. The technical review was sponsored by the National Institute on Drug Abuse (NIDA). COPYRIGHT STATUS NIDA has obtained permission from the copyright holders to reproduce certain previously published material as noted in the text. Further reproduction of this copyrighted material is permitted only as part of a reprinting of the entire publication or chapter. For any other use, the copyright holder’s permission is required. All other material in this volume except quoted passages from copyrighted sources is in the public domain and may be used or reproduced without permission from the Institute or the authors. Citation of the source is appreciated. Opinions expressed in this volume are those of the authors and do not necessarily reflect the opinions or official policy of the National Institute on Drug Abuse or any other part of the U.S. Department of Health and Human Services. The U.S. Government does not endorse or favor any specific commercial product or company. -
Impact of Scientific Developments on the Chemical Weapons Convention (IUPAC Technical Report)*
Pure Appl. Chem., Vol. 85, No. 4, pp. 851–881, 2013. http://dx.doi.org/10.1351/PAC-REP-12-11-18 © 2013 IUPAC, Publication date (Web): 16 February 2013 Impact of scientific developments on the Chemical Weapons Convention (IUPAC Technical Report)* Katie Smallwood1, Ralf Trapp2, Robert Mathews3, Beat Schmidt4, and Leiv K. Sydnes5,‡ 1Independent Consultant, Geneva, Switzerland; 2International Disarmament Consultant, 74270 Chessenaz, France; 3Defence Science and Technology Organisation, Australia; 4Spiez Laboratory, 3700 Spiez, Switzerland; 5Department of Chemistry, University of Bergen, 5007 Bergen, Norway Abstract: This document represents the final report of discussions and conclusions arising from the workshop on Developments in Science and Technology Relevant to the Chemical Weapons Convention, held in Spiez, Switzerland in February 2012. Keywords: Chemical Weapons Convention; CWC; implementation; science and technology; Third Review Conference. CONTENTS 1. INTRODUCTION 2. SUMMARY OF FINDINGS AND RECOMMENDATIONS 2.1 Overall findings and recommendations 2.2 S&T advances and their impact on the scope of the CWC 2.3 Developments that affect the practical implementation of the CWC 2.4 S&T advances specifically relevant to verification 2.5 S&T advances specifically relevant to protection against CW 2.6 The evolution of the international S&T environment 2.7 Extending support for the CWC via outreach and education 3. THE WORKSHOP 3.1 Overview and background 3.2 Convergence of chemistry and biology 3.3 New synthesis and toxicological analysis methods 3.4 Developing new materials and delivery mechanisms 3.5 Technical discussion 3.6 Advances in industrial production methods 3.7 Chemical safety and security: Possession, transfer, and acquisition 3.8 Defense against CW agents 3.9 Chemical safety and security: Engaging the chemical sciences community *Sponsoring body: IUPAC Executive Committee: see more details on p. -
Cyanobacterial Toxins: Saxitoxins
WHO/SDE/WSH/xxxxx English only Cyanobacterial toxins: Saxitoxins Background document for development of WHO Guidelines for Drinking-water Quality and Guidelines for Safe Recreational Water Environments Version for Public Review Nov 2019 © World Health Organization 20XX Preface Information on cyanobacterial toxins, including saxitoxins, is comprehensively reviewed in a recent volume to be published by the World Health Organization, “Toxic Cyanobacteria in Water” (TCiW; Chorus & Welker, in press). This covers chemical properties of the toxins and information on the cyanobacteria producing them as well as guidance on assessing the risks of their occurrence, monitoring and management. In contrast, this background document focuses on reviewing the toxicological information available for guideline value derivation and the considerations for deriving the guideline values for saxitoxin in water. Sections 1-3 and 8 are largely summaries of respective chapters in TCiW and references to original studies can be found therein. To be written by WHO Secretariat Acknowledgements To be written by WHO Secretariat 5 Abbreviations used in text ARfD Acute Reference Dose bw body weight C Volume of drinking water assumed to be consumed daily by an adult GTX Gonyautoxin i.p. intraperitoneal i.v. intravenous LOAEL Lowest Observed Adverse Effect Level neoSTX Neosaxitoxin NOAEL No Observed Adverse Effect Level P Proportion of exposure assumed to be due to drinking water PSP Paralytic Shellfish Poisoning PST paralytic shellfish toxin STX saxitoxin STXOL saxitoxinol -
Biosafety Level 1 and Rdna Training
Biosafety Level 1and rDNA Training Office of Biological Safety Biosafety Level 1 and rDNA Training • Difference between Risk Group and Biosafety Level • NIH and UC policy on recombinant DNA • Work conducted at Biosafety Level 1 • UC Code of Conduct for researchers Biosafety Level 1 and rDNA Training What is the difference between risk group and biosafety level? Risk Groups vs Biosafety Level • Risk Groups: Assigned to infectious organisms by global agencies (NIH, CDC, WHO, etc.) • In US, only assigned to human pathogens (NIH) • Biosafety Level (BSL): How the organisms are managed/contained (increasing levels of protection) Risk Groups vs Biosafety Level • RG1: Not associated with disease in healthy adults (non‐pathogenic E. coli; S. cerevisiae) • RG2: Cause diseases not usually serious and are often treatable (S. aureus; Legionella; Toxoplasma gondii) • RG3: Serious diseases that may be treatable (Y. pestis; B. anthracis; Rickettsia rickettsii; HIV) • RG4: Serious diseases with no treatment/cure (Hemorrhagic fever viruses, e.g., Ebola; no bacteria) Risk Groups vs Biosafety Level • BSL‐1: Usually corresponds to RG1 – Good microbiological technique – No additional safety equipment required for biological work (may still need chemical/radiation protection) – Ability to destroy recombinant organisms (even if they are RG1) Risk Groups vs Biosafety Level • BSL‐2: Same as BSL‐1, PLUS… – Biohazard signs – Protective clothing (lab coat, gloves, eye protection, etc.) – Biosafety cabinet (BSC) for aerosols is recommended but not always required – Negative airflow into room is recommended, but not always required Risk Groups vs Biosafety Level • BSL‐3: Same as BSL‐2, PLUS… – Specialized clothing (respiratory protection, Tyvek, etc.) – Directional air flow is required. -
Biosafety Manual 2017
Biosafety Manual 2017 Revised 6/2017 Policy Statement It is the policy of Northern Arizona University (NAU) to provide a safe working environment. The primary responsibility for insuring safe conduct and conditions in the laboratory resides with the principal investigator. The Office of Biological Safety is committed to providing up-to-date information, training, and monitoring to the research and biomedical community concerning the safe conduct of biological, recombinant, and acute toxin research and the handling of biological materials in accordance with all pertinent local, state and federal regulations, guidelines, and laws. To that end, this manual is a resource, to be used in conjunction with the CDC and NIH guidelines, the NAU Select Agent Program, Biosafety in Microbiological and Biomedical Laboratories (BMBL), and other resource materials. Introduction This Biological Safety Manual is intended for use as a guidance document for researchers and clinicians who work with biological materials. It should be used in conjunction with the Laboratory-Specific Safety Manual, which provides more general safety information. These manuals describe policies and procedures that are required for the safe conduct of research at NAU. The NAU Personnel Policy on Safety 5.03 also provides guidance for safety in the workplace. Responsibilities In the academic research/teaching setting, the principal investigator (PI) is responsible for ensuring that all members of the laboratory are familiar with safe research practices. In the clinical laboratory setting, the faculty member who supervises the laboratory is responsible for safety practices. Lab managers, supervisors, technicians and others who provide supervisory roles in laboratories and clinical settings are responsible for overseeing the safety practices in laboratories and reporting any problems, accidents, and spills to the appropriate faculty member. -
Development and Validation of a Novel Approach for the Analysis of Marine Biotoxins
DEVELOPMENT AND VALIDATION OF A NOVEL APPROACH FOR THE ANALYSIS OF MARINE BIOTOXINS Bing Cheng Chai College of Engineering and Science Victoria University Submitted in fulfilment of the requirements of the degree of Doctor of Philosophy June 2017 ABSTRACT Harmful algal blooms (HABs) which can produce a variety of marine biotoxins are a prevalent and growing risk to public safety. The aim of this research was to investigate, evaluate, develop and validate an analytical method for the detection and quantitation of five important groups of marine biotoxins in shellfish tissue. These groups included paralytic shellfish toxins (PST), amnesic shellfish toxins (AST), diarrheic shellfish toxins (DST), azaspiracids (AZA) and neurotoxic shellfish toxins (NST). A novel tandem liquid chromatographic (LC) approach using hydrophilic interaction chromatography (HILIC), aqueous normal phase (ANP), reversed phase (RP) chromatography, tandem mass spectrometry (MSMS) and fluorescence spectroscopic detection (FLD) was designed and tested. During method development of the tandem LC setup, it was found that HILIC and ANP columns were unsuitable for the PSTs because of the lack of chromatographic separation power, precluding them from being used with MSMS detection. In addition, sensitivity for the PSTs at regulatory limits could not be achieved with MSMS detection, which led to a RP-FLD combination. The technique of RP-MSMS was found to be suitable for the remaining four groups of biotoxins. The final method was a combination of two RP columns coupled with FLD and MSMS detectors, with a valve switching program and injection program. A novel sample preparation method was also developed for the extraction and clean-up of biotoxins from mussels. -
SAXITOXIN ELISA a Competitive Enzyme Immunoassay For
SAXITOXIN ELISA (5191SAXI[5]04.20) A competitive enzyme immunoassay for screening and quantitative analysis of Saxitoxin in various matrices EUROPROXIMA SAXITOXIN ELISA A competitive enzyme immunoassay for screening and quantitative analysis of Saxitoxin in various matrices TABLE OF CONTENTS PAGE: Brief Information .............................................................. 2 1. Introduction ...................................................................... 2 2. Principle of Saxitoxin ELISA ............................................ 3 3. Specificity and Sensitivity ................................................ 4 4. Handling and Storage ...................................................... 5 5. Kit contents ...................................................................... 6 6. Equipment required but not provided .............................. 7 7. Precautions ...................................................................... 7 8. Sample preparation ......................................................... 8 9. Preparation of reagents ................................................... 8 10. Assay Procedure ............................................................. 10 11. Interpretation of results .................................................... 12 12. Literature .......................................................................... 13 13. Ordering information ........................................................ 13 14. Revision history ............................................................... -
Detecting and Decontaminating Chemical and Biological Agents
Making the UK safer: detecting and decontaminating chemical and biological agents Policy document 06/04 April 2004 ISBN 0 85403 598 2 This report can be found at www.royalsoc.ac.uk ISBN 0 85403 598 2 © The Royal Society 2004. Requests to reproduce all or part of this document should be submitted to: Science Advice Section The Royal Society 6–9 Carlton House Terrace London SW1Y 5AG email [email protected] ii | April 2004 | The Royal Society Making the UK safer Making the UK safer: detecting and decontaminating chemical and biological agents Contents Page Summary 1 1 Introduction 3 1.1 Background to project 3 1.2 Conduct of project 4 1.3 Types and properties of possible agents 4 1.4 Types of possible incident 5 2 Priorities, concepts of use and implementation of detection systems 7 2.1 Detection, identification and monitoring 7 2.2 Activities and decision-making at different stages of an incident 7 2.3 User requirements and concepts of use 10 2.4 Current detection and monitoring technologies 10 2.5 Issues needing to be addressed 11 2.6 Key detection system requirements 11 2.7 Validation and implementation 11 2.8 Impacts on detection 11 2.9 Conclusions and recommendations 12 3 Issues relating to sampling 13 3.1 Introduction 13 3.2 Sampling strategies 13 3.3 Sampling issues needing to be addressed 14 3.4 Distribution of agent 14 3.5 Air sampling 15 3.6 Biological agent release 15 3.7 Chemical agent release 16 3.8 Conclusions and recommendations 16 4 Current capabilities and future needs of detection 17 4.1 Technologies for detecting -
Patent Application Publication ( 10 ) Pub . No . : US 2019 / 0192440 A1
US 20190192440A1 (19 ) United States (12 ) Patent Application Publication ( 10) Pub . No. : US 2019 /0192440 A1 LI (43 ) Pub . Date : Jun . 27 , 2019 ( 54 ) ORAL DRUG DOSAGE FORM COMPRISING Publication Classification DRUG IN THE FORM OF NANOPARTICLES (51 ) Int . CI. A61K 9 / 20 (2006 .01 ) ( 71 ) Applicant: Triastek , Inc. , Nanjing ( CN ) A61K 9 /00 ( 2006 . 01) A61K 31/ 192 ( 2006 .01 ) (72 ) Inventor : Xiaoling LI , Dublin , CA (US ) A61K 9 / 24 ( 2006 .01 ) ( 52 ) U . S . CI. ( 21 ) Appl. No. : 16 /289 ,499 CPC . .. .. A61K 9 /2031 (2013 . 01 ) ; A61K 9 /0065 ( 22 ) Filed : Feb . 28 , 2019 (2013 .01 ) ; A61K 9 / 209 ( 2013 .01 ) ; A61K 9 /2027 ( 2013 .01 ) ; A61K 31/ 192 ( 2013. 01 ) ; Related U . S . Application Data A61K 9 /2072 ( 2013 .01 ) (63 ) Continuation of application No. 16 /028 ,305 , filed on Jul. 5 , 2018 , now Pat . No . 10 , 258 ,575 , which is a (57 ) ABSTRACT continuation of application No . 15 / 173 ,596 , filed on The present disclosure provides a stable solid pharmaceuti Jun . 3 , 2016 . cal dosage form for oral administration . The dosage form (60 ) Provisional application No . 62 /313 ,092 , filed on Mar. includes a substrate that forms at least one compartment and 24 , 2016 , provisional application No . 62 / 296 , 087 , a drug content loaded into the compartment. The dosage filed on Feb . 17 , 2016 , provisional application No . form is so designed that the active pharmaceutical ingredient 62 / 170, 645 , filed on Jun . 3 , 2015 . of the drug content is released in a controlled manner. Patent Application Publication Jun . 27 , 2019 Sheet 1 of 20 US 2019 /0192440 A1 FIG . -
Cyanobacterial Toxins: Saxitoxins
WHO/HEP/ECH/WSH/2020.8 Cyanobacterial toxins: saxitoxins Background document for development of WHO Guidelines for Drinking-water Quality and Guidelines for Safe Recreational Water Environments WHO/HEP/ECH/WSH/2020.8 © World Health Organization 2020 Some rights reserved. This work is available under the Creative Commons Attribution- NonCommercial-ShareAlike 3.0 IGO licence (CC BY-NC-SA 3.0 IGO; https://creativecommons.org/ licenses/by-nc-sa/3.0/igo). Under the terms of this licence, you may copy, redistribute and adapt the work for non-commercial purposes, provided the work is appropriately cited, as indicated below. In any use of this work, there should be no suggestion that WHO endorses any specific organization, products or services. The use of the WHO logo is not permitted. If you adapt the work, then you must license your work under the same or equivalent Creative Commons licence. If you create a translation of this work, you should add the following disclaimer along with the suggested citation: “This translation was not created by the World Health Organization (WHO). WHO is not responsible for the content or accuracy of this translation. The original English edition shall be the binding and authentic edition”. Any mediation relating to disputes arising under the licence shall be conducted in accordance with the mediation rules of the World Intellectual Property Organization (http://www.wipo.int/amc/en/ mediation/rules/). Suggested citation. Cyanobacterial toxins: saxitoxins. Background document for development of WHO Guidelines for drinking-water quality and Guidelines for safe recreational water environments. Geneva: World Health Organization; 2020 (WHO/HEP/ECH/WSH/2020.8). -
Phycotoxins of the Paralytic Shellfish Poison: Clinical Applications
Clinical Pharmacology and Translational Medicine © All rights are reserved by Néstor Lagos. Review Article ISSN-2572-7656 Phycotoxins of the Paralytic Shellfish Poison: Clinical Applications Néstor Lagos Biochemical Membrane Laboratory, Department of Physiology and Biophysics, Faculty of Medicine, University of Chile. exhibit potent biological activities. They are secondary metabolites, Introduction which are not vital to the plain metabolism and growth of the A drug is defined as an agent intended for use in the diagnosis, organism, but they are present in constrained taxonomic groups. mitigation, treatment, cure, or prevention of disease in humans or in Among the many algal secondary metabolites that have been identi- other animals. Certainly, the vast array of effective medical agents fied, an important number are potent biotoxins responsible for a available today represents one of our greatest scientific accompli- wide array of human illnesses. shments. It is difficulty to conceive our civilization devoid of these Four major illnesses associated with microalgae biotoxins and remarkable and beneficial agents. Drug, in the form of vegetation and with shellfish poisoning have been described: Paralytic shellfish minerals, have existed longer than humans. Human disease and the poisoning, Diarrheic shellfish poisoning, Amnesic shellfish poisoning instinct to survive have, through the ages, led to their discovery. and Neurotoxic shellfish poisoning [6, 7]. Paralytic shellfish poiso- Throughout history, the knowledge of drug and their application to ning, which shown as primary clinical symptom acute paralytic disease has always meant power. illness, poses the most serious threat to public health due to its high Searching for potential clinical applications, poisonous substances mortality rate in mammals [4]. -
Bulletin 80 13 Dec.P65
THE CBW CONVENTIONS BULLETIN News, Background and Comment on Chemical and Biological Weapons Issues ISSUE NO. 80 SEPTEMBER 2008 Quarterly Journal of the Harvard Sussex Program on CBW Armament and Arms Limitation BRINGING THE CBW CONVENTIONS CLOSER TOGETHER Julian Perry Robinson, Harvard Sussex Program In 1968, for reasons that are still not entirely clear, the United – people, other animals and plants – on a giant scale. The Kingdom proposed that biological weapons (BW) and Shady Grove field trials conducted by the United States off chemical weapons (CW) should in future be treated separately Johnston Atoll in the Pacific Ocean during February and at the Geneva disarmament conference, and that the talks March 1964 are said to have demonstrated that, against caged should first concentrate on BW. That is what happened, rhesus monkeys, one single-seat aircraft could establish gradually. The international agreement that already existed disease-causing dosages of bacterial aerosol at sea level over in the field, the 1925 Geneva Protocol, had taken chemical nearly five thousand square kilometres. That was a biological and biological weapons (CBW) together. Their new separation weapon, but, as is described below, similar areas of led to the 1972 Biological & Toxin Weapons Convention (the effectiveness were anticipated for future chemical weapons BWC) and then the 1993 Chemical Weapons Convention as well. Urban areas of like size – the habitat of maybe (CWC), so in fact both types of weapon became outlawed, millions of people — might be no less vulnerable. So, as and it is possible that neither would have been without that casualty agents, there may in principle be some comparability parting of the ways.